Rorug04G0043400

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000521
Physical Location & Seq
Forward (+)
6779410 .. 6781245
1836 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0043400.1

Sequence Viewer

Length: 1836 bp
ATGAGGAGCATCCTAGAAGGTCTAGGAGAAAATGTCTCCGGAGCTGGTGTTACTCAGATTTTGAGCAGAATTCAGCAAGTACTTAAAGATAAGAGCTGTCTGATTGTCATGGATGATGTGTGGAATCAAACAGATGTTGATTGGTGGACCAACCTCTGCTCTGTTATACCCAAACAAAGCAGCTGCATCATCGTTACAACTAGAAATGAAGAAGCTGTAATTGGTATGGGAGTTGAAAGCTCACGAATCCACCGTCCCAAGACTCTTAATGATGAGGAAAGCTGGCGCTTGTTCTCCAAGTTTGCATTTCCTACAAGCAAGGGATTGTGCCCGGAGGACAGGCTTGAGAAATTAGGAAAAGAGCTCTTGAAGAAGTGTGGTGGACTCCCACTCGCAATCAAGACTATAGGATCCTTGTTGGCATCAAAGGTCGACTCCCCTTCTCAGTGGAGACGAGTTCTCGAGAGCTTTCATGCTTTGACAACAGAGGGAAAAACTAGCTCAGTTATGGCCTCTTTGCGATTGAGCTATGATGAACTCCCGCCTGGTCTGAAGCAATGCCTGCTGTGTTTCTCCATCTATCCTGAAGACTATGAGATACGTGCTGATCAGTTAATCCATTGGTGGATTGGAGAAGGCCTCGTGCAAAGCAAGGGCTCAAGAACAGCAGTAGAAGTGGGATATGAGTATCTTGCGGAACTGATAAGCAGATGCTTGGTTGAAATTGTGCAGCAGAGAGGATTTGATGGGAGAGTCTACAAGTGCAAGATTCATGATATGGTCCGGGAACTGATAATAATGATTGCAGAGGAGGAGGAATTATGCGGCTTTGACGAACAAGGCAGACAGAAATTGACAGCAAATACTAGGTGGTTATGCTTTATTGATGAAATGGAGGAGAAGTCCTTGAAACGTAGTTCAAAGCTCAGGGCATTGTTGATGATGTCAAGCCGCAGATTTGACTTTGAAAGGAATTCTCTGCGATCCCTCAGAATGTTGGATTTATCAAACTGCACTGTGGATGAGCATTGTGTGAAGGATCTCTTCAGCTGGATCAGCTCCCTTAAGCGCTTAGCATCTTTGAACTTAAGTGGTATTCAGGCTCTGAAAGAAGTGCCATCTTCAATTCATAAACTCCTCAACCTCCAACTTTTACTTCTAACAGGTTGCAGCAATCTAGAAAAAATACACCCTTCCATCACTAATTTGAAGAAGCTGATTATTTTGGACCTAGGCGGGTGTCCAATCCAATACTTACCTCAGGGATTGGGAAGGCTTTCTTATCTTCAAGAGTTCTCCGGATTCAAGATTGTGAGTCAGCCTAGAATGCCATGTTTCCAGCTTCTTGAGATTAGGGAACTGATCAACCTGAGAGTACTGCGAATACATGTAAGCAGTACCACAGTTATTGCAGACAATGAACTTGATGTCCTCTCTCAGCTTAGAAGGCTCAATGTTCTGGCTATCGACGCAGAAGATTGTAGAAATAACAATGTTTTGGAAATGCTAGAAAGGCTGACCCCTCCTCCAAGCCTCCAAGAGCTGTATCTTAGGAATTACAAGAAGGAAACTCTGCCTAGCTGGGTTAATCCCGGACAGCTTTCAAGATTGCAGTATCTTTGCATCGAGAATGGAGACCTTGTCAAGCTAAGCAGTGGTCAAACCACCTGGAACCTTGAAGGCCTGTGTCTCAAGTACTTGATGAGGCTAGAGGTAGACTGGAAGGACTTGGAGAAGGATATGCCTGTACTCCACTACATGGAGATCAGTCATTGTTACAACCTGAAGGATTTCCCATGTTCAGTTATGGAACCTGGGGTCTGGAGAAAGAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

611

Amino Acids

69.89

Weight (kDa)

6.81

Isoelectric Point (pI)

48.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 19 - 106 4.6e-17 NB-ARC domain
WHD_DRP PF23559 193 - 265 1.4e-20 Disease resistance protein Winged helix domain
LRR_14 PF23598 322 - 543 3.9e-27 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 459 - 552 9.9e-09 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000521)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g09990 FvH4_4g09990 FvH4_4g09990 FvH4_4g09990 FvH4_7g32860 FvH4_7g32860 FvH4_7g32860 FvH4_7g32860
malus_domestica MD11G1148000.v1.1 MD13G1266200.v1.1 MD14G1231800.v1.1
prunus_persica Prupe.1G090100_v2.0.a1 Prupe.1G090100_v2.0.a1 Prupe.1G090100_v2.0.a1 Prupe.1G090100_v2.0.a1 Prupe.1G090100_v2.0.a1 Prupe.1G090300_v2.0.a1 Prupe.1G090300_v2.0.a1 Prupe.1G090300_v2.0.a1 Prupe.1G090300_v2.0.a1 Prupe.1G090300_v2.0.a1
pyrus_communis pycom13g23330
rosa_chinensis RchiOBHm_Chr4g0403401 RchiOBHm_Chr4g0403411 RchiOBHm_Chr4g0403421 RchiOBHm_Chr4g0403461 RchiOBHm_Chr4g0403631 RchiOBHm_Chr7g0223601 RchiOBHm_Chr7g0238161
rosa_laevigata RLG00000000937 RLG00000001957 RLG00000008965 RLG00000008975 RLG00000008976 RLG00000008978
rosa_multiflora Rmu_co8335743.1_g000001 Rmu_sc0005734.1_g000001 Rmu_sc0007727.1_g000011 Rmu_sc0011775.1_g000002 Rmu_sc0011775.1_g000005 Rmu_sc0028062.1_g000002
rosa_roxburghii Rroxscaffold_3G00223300 Rroxscaffold_5G00348050 Rroxscaffold_5G00348060 Rroxscaffold_5G00348120 Rroxscaffold_5G00348240
rosa_rugosa Rorug04G0042300 Rorug04G0042400 Rorug04G0042500 Rorug04G0042600.1 Rorug04G0042700 Rorug04G0042800 Rorug04G0042900 Rorug04G0043000 Rorug04G0043100 Rorug04G0043200 Rorug04G0043300 Rorug04G0043400 Rorug04G0044700 Rorug07G0310600 Rorug07G0310700 Rorug07G0310700
rosa_samantha Rh4AG118700 Rh4AG118800 Rh4AG118900 Rh4AG119000 Rh4BG111600 Rh4BG111800 Rh4BG111900 Rh4BG112900 Rh4CG126600 Rh4CG126900 Rh4CG127800 Rh4DG111300 Rh4DG111400 Rh4DG111500 Rh7AG466500 Rh7BG352200 Rh7BG436900 Rh7CG484200 Rh7DG452600
rosa_wichuraiana Rw4G009630 Rw4G009640 Rw4G009650 Rw4G009710 Rw7G038620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1759
AccI GTMKAC 3 cut(s) 432, 756, 1716
AccIII TCCGGA 2 cut(s) 38, 1298
AciI CCGC 5 cut(s) 542, 695, 825, 952, 1236
AclWI GGATC 5 cut(s) 405, 418, 978, 1047, 1061
AcsI RAATTY 2 cut(s) 69, 973
AcuI CTGAAG 4 cut(s) 572, 606, 1030, 1805
AfaI GTAC 5 cut(s) 81, 1377, 1399, 1697, 1749
AfeI AGCGCT 1 cut(s) 1070
AfiI CCNNNNNNNGG 1 cut(s) 1759
AflII CTTAAG 2 cut(s) 1064, 1087
AflIII ACRYGT 1 cut(s) 1387
AjnI CCWGG 3 cut(s) 544, 1667, 1813
AjuI GAANNNNNNNTTGG 4 cut(s) 204, 236, 1140, 1172
AloI GAACNNNNNNTCC 2 cut(s) 1413, 1445
Alw21I GWGCWC 1 cut(s) 366
Alw26I GTCTC 4 cut(s) 40, 445, 1629, 1694
AlwI GGATC 5 cut(s) 405, 418, 978, 1047, 1061
AlwNI CAGNNNCTG 1 cut(s) 1105
Ama87I CYCGRG 1 cut(s) 461
Aor13HI TCCGGA 2 cut(s) 38, 1298
Aor51HI AGCGCT 1 cut(s) 1070
AoxI GGCC 3 cut(s) 510, 637, 1681
ApeKI GCWGC 4 cut(s) 180, 183, 730, 1170
ApoI RAATTY 2 cut(s) 69, 973
ArsI GACNNNNNNTTYG 2 cut(s) 238, 270
Asp700I GAANNNNTTC 2 cut(s) 1276, 1790
AspA2I CCTAGG 1 cut(s) 1231
AspLEI GCGC 2 cut(s) 288, 1071
AspS9I GGNCC 3 cut(s) 147, 781, 1228
AsuC2I CCSGG 3 cut(s) 332, 785, 1593
AvaI CYCGRG 1 cut(s) 461
AvaII GGWCC 3 cut(s) 147, 781, 1228
AvrII CCTAGG 1 cut(s) 1231
AxyI CCTNAGG 1 cut(s) 1260
BaeGI GKGCMC 1 cut(s) 332
BamHI GGATCC 1 cut(s) 410
BanII GRGCYC 2 cut(s) 366, 659
BauI CACGAG 1 cut(s) 641
BbsI GAAGAC 1 cut(s) 594
Bbv12I GWGCWC 1 cut(s) 366
BbvI GCAGC 4 cut(s) 170, 192, 742, 1182
BccI CCATC 4 cut(s) 584, 740, 1126, 1205
BciT130I CCWGG 3 cut(s) 546, 1669, 1815
BclI TGATCA 2 cut(s) 607, 1362
BcnI CCSGG 3 cut(s) 332, 785, 1593
BcoDI GTCTC 4 cut(s) 40, 445, 1629, 1694
BfmI CTRYAG 1 cut(s) 405
BfoI RGCGCY 2 cut(s) 289, 1072
BfrI CTTAAG 2 cut(s) 1064, 1087
BisI GCNGC 6 cut(s) 181, 184, 731, 826, 952, 1171
BlnI CCTAGG 1 cut(s) 1231
BlpI GCTNAGC 2 cut(s) 1072, 1649
BlsI GCNGC 6 cut(s) 182, 185, 732, 827, 953, 1172
BmcAI AGTACT 3 cut(s) 81, 1377, 1697
Bme1390I CCNGG 6 cut(s) 332, 546, 785, 1593, 1669, 1815
Bme18I GGWCC 3 cut(s) 147, 781, 1228
BmeT110I CYCGRG 1 cut(s) 461
BmgT120I GGNCC 3 cut(s) 147, 781, 1228
BmiI GGNNCC 3 cut(s) 412, 1673, 1812
BmrFI CCNGG 6 cut(s) 332, 546, 785, 1593, 1669, 1815
BmsI GCATC 6 cut(s) 18, 195, 431, 701, 1085, 1632
BpiI GAAGAC 1 cut(s) 594
BplI GAGNNNNNCTC 2 cut(s) 624, 656
Bpu10I CCTNAGC 1 cut(s) 926
Bpu1102I GCTNAGC 2 cut(s) 1072, 1649
BpuEI CTTGAG 4 cut(s) 365, 643, 1367, 1676
BpuMI CCSGG 3 cut(s) 332, 785, 1593
BsaAI YACGTR 1 cut(s) 602
BsaI GGTCTC 1 cut(s) 1629
BsaJI CCNNGG 2 cut(s) 1231, 1814
BsaWI WCCGGW 2 cut(s) 38, 1298
Bsc4I CCNNNNNNNGG 1 cut(s) 1759
Bse1I ACTGG 1 cut(s) 1724
Bse21I CCTNAGG 1 cut(s) 1260
Bse3DI GCAATG 1 cut(s) 563
BseAI TCCGGA 2 cut(s) 38, 1298
BseBI CCWGG 3 cut(s) 546, 1669, 1815
BseDI CCNNGG 2 cut(s) 1231, 1814
BseGI GGATG 3 cut(s) 9, 118, 1027
BseLI CCNNNNNNNGG 1 cut(s) 1759
BseMI GCAATG 1 cut(s) 563
BseMII CTCAG 8 cut(s) 68, 458, 516, 940, 1003, 1274, 1361, 1451
BseNI ACTGG 1 cut(s) 1724
BseRI GAGGAG 6 cut(s) 19, 824, 827, 911, 1127, 1515
BseSI GKGCMC 1 cut(s) 332
BseXI GCAGC 4 cut(s) 170, 192, 742, 1182
BseYI CCCAGC 1 cut(s) 1581
BsgI GTGCAG 2 cut(s) 749, 997
BshFI GGCC 3 cut(s) 512, 639, 1683
BsiHKAI GWGCWC 1 cut(s) 366
BsiHKCI CYCGRG 1 cut(s) 461
BsiSI CCGG 5 cut(s) 39, 332, 784, 1299, 1593
BslFI GGGAC 1 cut(s) 240
BslI CCNNNNNNNGG 1 cut(s) 1759
BsmAI GTCTC 4 cut(s) 40, 445, 1629, 1694
BsmBI CGTCTC 1 cut(s) 445
BsmFI GGGAC 1 cut(s) 240
BsmI GAATGC 1 cut(s) 1332
BsnI GGCC 3 cut(s) 512, 639, 1683
Bso31I GGTCTC 1 cut(s) 1629
BsoBI CYCGRG 1 cut(s) 461
Bsp1286I GDGCHC 3 cut(s) 332, 366, 659
Bsp13I TCCGGA 2 cut(s) 38, 1298
Bsp143I GATC 7 cut(s) 410, 607, 983, 1039, 1053, 1362, 1764
Bsp1720I GCTNAGC 2 cut(s) 1072, 1649
BspACI CCGC 5 cut(s) 542, 695, 825, 952, 1236
BspANI GGCC 3 cut(s) 512, 639, 1683
BspCNI CTCAG 8 cut(s) 67, 457, 515, 939, 1002, 1273, 1362, 1450
BspEI TCCGGA 2 cut(s) 38, 1298
BspHI TCATGA 1 cut(s) 772
BspLI GGNNCC 3 cut(s) 412, 1673, 1812
BspPI GGATC 5 cut(s) 405, 418, 978, 1047, 1061
BspTI CTTAAG 2 cut(s) 1064, 1087
BspTNI GGTCTC 1 cut(s) 1629
BsrDI GCAATG 1 cut(s) 563
BsrI ACTGG 1 cut(s) 1724
BssECI CCNNGG 2 cut(s) 1231, 1814
BssMI GATC 7 cut(s) 410, 607, 983, 1039, 1053, 1362, 1764
BssSI CACGAG 1 cut(s) 641
BssT1I CCWWGG 1 cut(s) 1231
Bst2BI CACGAG 1 cut(s) 641
Bst2UI CCWGG 3 cut(s) 546, 1669, 1815
Bst4CI ACNGT 3 cut(s) 254, 1018, 1405
Bst6I CTCTTC 1 cut(s) 1049
BstAFI CTTAAG 2 cut(s) 1064, 1087
BstAPI GCANNNNNTGC 1 cut(s) 562
BstBAI YACGTR 1 cut(s) 602
BstC8I GCNNGC 2 cut(s) 284, 563
BstF5I GGATG 3 cut(s) 9, 118, 1027
BstH2I RGCGCY 2 cut(s) 289, 1072
BstHHI GCGC 2 cut(s) 288, 1071
BstKTI GATC 7 cut(s) 413, 610, 986, 1042, 1056, 1365, 1767
BstMAI GTCTC 4 cut(s) 40, 445, 1629, 1694
BstMBI GATC 7 cut(s) 410, 607, 983, 1039, 1053, 1362, 1764
BstMWI GCNNNNNNNGC 6 cut(s) 562, 1056, 1327, 1447, 1469, 1513
BstNI CCWGG 3 cut(s) 546, 1669, 1815
BstNSI RCATGY 1 cut(s) 1391
BstSCI CCNGG 6 cut(s) 330, 544, 783, 1591, 1667, 1813
BstSFI CTRYAG 1 cut(s) 405
BstSLI GKGCMC 1 cut(s) 332
BstV1I GCAGC 4 cut(s) 170, 192, 742, 1182
BstV2I GAAGAC 1 cut(s) 594
BstX2I RGATCY 2 cut(s) 410, 1039
BstYI RGATCY 2 cut(s) 410, 1039
Bsu36I CCTNAGG 1 cut(s) 1260
BsuRI GGCC 3 cut(s) 512, 639, 1683
BtsCI GGATG 3 cut(s) 9, 118, 1027
BtsI GCAGTG 1 cut(s) 1660
BtsIMutI CAGTG 3 cut(s) 452, 1014, 1660
Cac8I GCNNGC 2 cut(s) 284, 563
CaiI CAGNNNCTG 1 cut(s) 1105
CciI TCATGA 1 cut(s) 772
CfoI GCGC 2 cut(s) 288, 1071
Cfr13I GGNCC 3 cut(s) 147, 781, 1228
CseI GACGC 1 cut(s) 1478
Csp6I GTAC 5 cut(s) 80, 1376, 1398, 1696, 1748
CspCI CAANNNNNGTGG 2 cut(s) 1390, 1425
CviAII CATG 7 cut(s) 109, 473, 773, 1332, 1388, 1759, 1797
CviQI GTAC 5 cut(s) 80, 1376, 1398, 1696, 1748
DpnI GATC 7 cut(s) 412, 609, 985, 1041, 1055, 1364, 1766
DpnII GATC 7 cut(s) 410, 607, 983, 1039, 1053, 1362, 1764
Eam1104I CTCTTC 1 cut(s) 1049
EarI CTCTTC 1 cut(s) 1049
Ecl136II GAGCTC 1 cut(s) 364
Eco130I CCWWGG 1 cut(s) 1231
Eco147I AGGCCT 2 cut(s) 639, 1683
Eco24I GRGCYC 2 cut(s) 366, 659
Eco31I GGTCTC 1 cut(s) 1629
Eco47I GGWCC 3 cut(s) 147, 781, 1228
Eco47III AGCGCT 1 cut(s) 1070
Eco53kI GAGCTC 1 cut(s) 364
Eco57I CTGAAG 4 cut(s) 572, 606, 1030, 1805
Eco81I CCTNAGG 1 cut(s) 1260
Eco88I CYCGRG 1 cut(s) 461
EcoICRI GAGCTC 1 cut(s) 364
EcoRI GAATTC 2 cut(s) 69, 973
EcoRII CCWGG 3 cut(s) 544, 1667, 1813
EcoT14I CCWWGG 1 cut(s) 1231
EcoT38I GRGCYC 2 cut(s) 366, 659
ErhI CCWWGG 1 cut(s) 1231
Esp3I CGTCTC 1 cut(s) 445
FaeI CATG 7 cut(s) 112, 476, 776, 1335, 1391, 1762, 1800
FalI AAGNNNNNCTT 4 cut(s) 1028, 1060, 1264, 1296
FaqI GGGAC 1 cut(s) 240
FatI CATG 7 cut(s) 108, 472, 772, 1331, 1387, 1758, 1796
FauI CCCGC 2 cut(s) 549, 1229
FbaI TGATCA 2 cut(s) 607, 1362
FblI GTMKAC 3 cut(s) 432, 756, 1716
Fnu4HI GCNGC 6 cut(s) 181, 184, 731, 826, 952, 1171
FokI GGATG 2 cut(s) 125, 1034
FriOI GRGCYC 2 cut(s) 366, 659
Fsp4HI GCNGC 6 cut(s) 181, 184, 731, 826, 952, 1171
GlaI GCGC 2 cut(s) 287, 1070
GluI GCNGC 6 cut(s) 181, 184, 731, 826, 952, 1171
GsaI CCCAGC 1 cut(s) 1585
HaeII RGCGCY 2 cut(s) 289, 1072
HaeIII GGCC 3 cut(s) 512, 639, 1683
HapII CCGG 5 cut(s) 39, 332, 784, 1299, 1593
HgaI GACGC 1 cut(s) 1478
HhaI GCGC 2 cut(s) 288, 1071
Hin1II CATG 7 cut(s) 112, 476, 776, 1335, 1391, 1762, 1800
Hin6I GCGC 2 cut(s) 286, 1069
HinP1I GCGC 2 cut(s) 286, 1069
HincII GTYRAC 1 cut(s) 433
HindII GTYRAC 1 cut(s) 433
HinfI GANTC 9 cut(s) 124, 246, 262, 384, 434, 753, 769, 1302, 1315
HpaII CCGG 5 cut(s) 39, 332, 784, 1299, 1593
Hpy166II GTNNAC 5 cut(s) 147, 383, 433, 757, 1717
Hpy188I TCNGA 5 cut(s) 57, 102, 552, 992, 1107
Hpy8I GTNNAC 5 cut(s) 147, 383, 433, 757, 1717
Hpy99I CGWCG 1 cut(s) 1472
HpyCH4III ACNGT 3 cut(s) 254, 1018, 1405
HpyCH4IV ACGT 2 cut(s) 601, 913
HpyF10VI GCNNNNNNNGC 6 cut(s) 562, 1056, 1327, 1447, 1469, 1513
HpySE526I ACGT 2 cut(s) 601, 913
Hsp92II CATG 7 cut(s) 112, 476, 776, 1335, 1391, 1762, 1800
HspAI GCGC 2 cut(s) 286, 1069
Kpn2I TCCGGA 2 cut(s) 38, 1298
Ksp22I TGATCA 2 cut(s) 607, 1362
Kzo9I GATC 7 cut(s) 410, 607, 983, 1039, 1053, 1362, 1764
LmnI GCTCC 3 cut(s) 6, 41, 1064
Lsp1109I GCAGC 4 cut(s) 170, 192, 742, 1182
LweI GCATC 6 cut(s) 18, 195, 431, 701, 1085, 1632
MaeII ACGT 2 cut(s) 601, 913
MaeIII GTNAC 3 cut(s) 49, 193, 1775
MalI GATC 7 cut(s) 412, 609, 985, 1041, 1055, 1364, 1766
MboI GATC 7 cut(s) 410, 607, 983, 1039, 1053, 1362, 1764
MboII GAAGA 8 cut(s) 221, 382, 599, 1036, 1113, 1222, 1277, 1487
MflI RGATCY 2 cut(s) 410, 1039
MhlI GDGCHC 3 cut(s) 332, 366, 659
MlyI GAGTC 5 cut(s) 256, 378, 428, 762, 1324
MmeI TCCRAC 2 cut(s) 978, 1171
MroI TCCGGA 2 cut(s) 38, 1298
MroXI GAANNNNTTC 2 cut(s) 1276, 1790
MseI TTAA 7 cut(s) 84, 267, 614, 1065, 1088, 1587, 1834
MspA1I CMGCKG 2 cut(s) 183, 1050
MspCI CTTAAG 2 cut(s) 1064, 1087
MspI CCGG 5 cut(s) 39, 332, 784, 1299, 1593
MspR9I CCNGG 6 cut(s) 332, 546, 785, 1593, 1669, 1815
Mva1269I GAATGC 1 cut(s) 1332
MvaI CCWGG 3 cut(s) 546, 1669, 1815
MwoI GCNNNNNNNGC 6 cut(s) 562, 1056, 1327, 1447, 1469, 1513
NciI CCSGG 3 cut(s) 332, 785, 1593
NdeII GATC 7 cut(s) 410, 607, 983, 1039, 1053, 1362, 1764
NlaIII CATG 7 cut(s) 112, 476, 776, 1335, 1391, 1762, 1800
NlaIV GGNNCC 3 cut(s) 412, 1673, 1812
NspI RCATGY 1 cut(s) 1391
PaeR7I CTCGAG 1 cut(s) 461
PagI TCATGA 1 cut(s) 772
PceI AGGCCT 2 cut(s) 639, 1683
PciI ACATGT 1 cut(s) 1387
PcsI WCGNNNNNNNCGW 1 cut(s) 250
PctI GAATGC 1 cut(s) 1332
PdmI GAANNNNTTC 2 cut(s) 1276, 1790
PfeI GAWTC 4 cut(s) 124, 246, 769, 1302
PflFI GACNNNGTC 1 cut(s) 1640
PflMI CCANNNNNTGG 1 cut(s) 1759
PfoI TCCNGGA 2 cut(s) 783, 1591
PkrI GCNGC 6 cut(s) 182, 185, 732, 827, 953, 1172
PleI GAGTC 5 cut(s) 256, 378, 428, 761, 1323
PpsI GAGTC 5 cut(s) 256, 378, 428, 761, 1323
Ppu21I YACGTR 1 cut(s) 602
PscI ACATGT 1 cut(s) 1387
Psp124BI GAGCTC 1 cut(s) 366
Psp6I CCWGG 3 cut(s) 544, 1667, 1813
PspFI CCCAGC 1 cut(s) 1581
PspGI CCWGG 3 cut(s) 544, 1667, 1813
PspN4I GGNNCC 3 cut(s) 412, 1673, 1812
PspPI GGNCC 3 cut(s) 147, 781, 1228
PstNI CAGNNNCTG 1 cut(s) 1105
PsuI RGATCY 2 cut(s) 410, 1039
PsyI GACNNNGTC 1 cut(s) 1640
PvuII CAGCTG 2 cut(s) 183, 1050
RsaI GTAC 5 cut(s) 81, 1377, 1399, 1697, 1749
RsaNI GTAC 5 cut(s) 80, 1376, 1398, 1696, 1748
SacI GAGCTC 1 cut(s) 366
SalI GTCGAC 1 cut(s) 431
SaqAI TTAA 7 cut(s) 84, 267, 614, 1065, 1088, 1587, 1834
SatI GCNGC 6 cut(s) 181, 184, 731, 826, 952, 1171
Sau3AI GATC 7 cut(s) 410, 607, 983, 1039, 1053, 1362, 1764
Sau96I GGNCC 3 cut(s) 147, 781, 1228
ScaI AGTACT 3 cut(s) 81, 1377, 1697
SchI GAGTC 5 cut(s) 256, 378, 428, 762, 1324
ScrFI CCNGG 6 cut(s) 332, 546, 785, 1593, 1669, 1815
SduI GDGCHC 3 cut(s) 332, 366, 659
SfaNI GCATC 6 cut(s) 18, 195, 431, 701, 1085, 1632
SfcI CTRYAG 1 cut(s) 405
Sfr274I CTCGAG 1 cut(s) 461
SinI GGWCC 3 cut(s) 147, 781, 1228
SlaI CTCGAG 1 cut(s) 461
SmlI CTYRAG 7 cut(s) 344, 461, 658, 1064, 1087, 1346, 1691
SmoI CTYRAG 7 cut(s) 344, 461, 658, 1064, 1087, 1346, 1691
SseBI AGGCCT 2 cut(s) 639, 1683
SsiI CCGC 5 cut(s) 542, 695, 825, 952, 1236
SstI GAGCTC 1 cut(s) 366
StuI AGGCCT 2 cut(s) 639, 1683
StyD4I CCNGG 6 cut(s) 330, 544, 783, 1591, 1667, 1813
StyI CCWWGG 1 cut(s) 1231
TaaI ACNGT 3 cut(s) 254, 1018, 1405
TaiI ACGT 2 cut(s) 604, 916
TaqI TCGA 4 cut(s) 432, 462, 1467, 1626
TatI WGTACW 4 cut(s) 79, 1375, 1695, 1747
TauI GCSGC 2 cut(s) 828, 954
TfiI GAWTC 4 cut(s) 124, 246, 769, 1302
Tru1I TTAA 7 cut(s) 84, 267, 614, 1065, 1088, 1587, 1834
Tru9I TTAA 7 cut(s) 84, 267, 614, 1065, 1088, 1587, 1834
TscAI CASTG 3 cut(s) 452, 1021, 1660
TseI GCWGC 4 cut(s) 180, 183, 730, 1170
TspDTI ATGAA 7 cut(s) 222, 461, 549, 761, 903, 1118, 1434
TspRI CASTG 3 cut(s) 452, 1021, 1660
Tth111I GACNNNGTC 1 cut(s) 1640
Van91I CCANNNNNTGG 1 cut(s) 1759
Vha464I CTTAAG 2 cut(s) 1064, 1087
VpaK11BI GGWCC 3 cut(s) 147, 781, 1228
XapI RAATTY 2 cut(s) 69, 973
XbaI TCTAGA 1 cut(s) 1177
XceI RCATGY 1 cut(s) 1391
XcmI CCANNNNNNNNNTGG 1 cut(s) 626
XhoI CTCGAG 1 cut(s) 461
XmaJI CCTAGG 1 cut(s) 1231
XmiI GTMKAC 3 cut(s) 432, 756, 1716
XmnI GAANNNNTTC 2 cut(s) 1276, 1790
ZrmI AGTACT 3 cut(s) 81, 1377, 1697
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.