Rmu_sc0007727.1_g000011

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0007727.1
OG0000521
Physical Location & Seq
Reverse (-)
48570 .. 52007
3438 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0007727.1_g000011.1.cds

Sequence Viewer

Length: 1329 bp
atggctgacgaagaacaatctgctggtcttgaatcacccttgattccagttcagccccaacaacatgcaatacaatcaacagggggacaattcaccaaaggcgaattgttcggggaactgaagaagcagttattgttggcagggccgctggtatcatcaaatttcttgttgtttggtatgcaggttatttcagtcatgtatgttggtcatcttggggagctatcacttgcaggtgctgctatggccacttcatttgcatctgtgactggtttgagcttgataataggaatgggtagcgcattagacacattttgcgggcagtcatatggagcaaaacagtatcatatggttgggatacacttgcagagggcaatgcttgttcttctgctggcctgcattcctattgcaactatatggttcaatgcgggctacattcttcaatccttgggtcaagatccagaaattgctgcagctgctggaaactatgctcgtttcttgataccttgcatttttgcttacgcagtccaacaatgtcattctagattcttgcaaacccaaagcaatgtggttcctatgatagttagcactggcacagcaacactagtgcacttgcttgtctgttggcttctggtatataagacaagccttggatatagaggagctgctgtcgcaatctccatctcatattggatcaatgcgttgttcttagttgtttatatcagagtttctccctcttgtaaacacacatggacgggattttcaaaggaggcatttcatggaattcccaacttcataaaattatctatcccttcagctataatgatcagcctagaaatctggtcattcgaattgatggttctcttatccggttttcttccaaatccacagcttgaaacctcagtgctatcaatcagccttaacacatgctccatgatatacatgattcctcttgcattcgccgctgcagcaagcacaagagtttcaaatcaactgggtgccgggcaaccacgacttgctcgtctagcagtaggtgttgcactatgcattgttgttaccgaaggtattatggctgctgctattctgatactgggtcgaaaagtttggggatactgttacagcagtgaaaaggaagtgatcaattatgttggacaaatgcttattttggttgcggtatcccacttttttgatggacttcaatctgtgctttcaggcgaactcaccgtgcacaccttcaattctgatcaactgagttgtcgctttgatggtgacgctgtccgggatgtccaaagtgttcgtccggcaaccgcagctcctgaaaccactgcctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

442

Amino Acids

47.53

Weight (kDa)

6.54

Isoelectric Point (pI)

33.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000521)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g09990 FvH4_4g09990 FvH4_4g09990 FvH4_4g09990 FvH4_7g32860 FvH4_7g32860 FvH4_7g32860 FvH4_7g32860
malus_domestica MD11G1148000.v1.1 MD13G1266200.v1.1 MD14G1231800.v1.1
prunus_persica Prupe.1G090100_v2.0.a1 Prupe.1G090100_v2.0.a1 Prupe.1G090100_v2.0.a1 Prupe.1G090100_v2.0.a1 Prupe.1G090100_v2.0.a1 Prupe.1G090300_v2.0.a1 Prupe.1G090300_v2.0.a1 Prupe.1G090300_v2.0.a1 Prupe.1G090300_v2.0.a1 Prupe.1G090300_v2.0.a1
pyrus_communis pycom13g23330
rosa_chinensis RchiOBHm_Chr4g0403401 RchiOBHm_Chr4g0403411 RchiOBHm_Chr4g0403421 RchiOBHm_Chr4g0403461 RchiOBHm_Chr4g0403631 RchiOBHm_Chr7g0223601 RchiOBHm_Chr7g0238161
rosa_laevigata RLG00000000937 RLG00000001957 RLG00000008965 RLG00000008975 RLG00000008976 RLG00000008978
rosa_multiflora Rmu_co8335743.1_g000001 Rmu_sc0005734.1_g000001 Rmu_sc0007727.1_g000011 Rmu_sc0011775.1_g000002 Rmu_sc0011775.1_g000005 Rmu_sc0028062.1_g000002
rosa_roxburghii Rroxscaffold_3G00223300 Rroxscaffold_5G00348050 Rroxscaffold_5G00348060 Rroxscaffold_5G00348120 Rroxscaffold_5G00348240
rosa_rugosa Rorug04G0042300 Rorug04G0042400 Rorug04G0042500 Rorug04G0042600.1 Rorug04G0042700 Rorug04G0042800 Rorug04G0042900 Rorug04G0043000 Rorug04G0043100 Rorug04G0043200 Rorug04G0043300 Rorug04G0043400 Rorug04G0044700 Rorug07G0310600 Rorug07G0310700 Rorug07G0310700
rosa_samantha Rh4AG118700 Rh4AG118800 Rh4AG118900 Rh4AG119000 Rh4BG111600 Rh4BG111800 Rh4BG111900 Rh4BG112900 Rh4CG126600 Rh4CG126900 Rh4CG127800 Rh4DG111300 Rh4DG111400 Rh4DG111500 Rh7AG466500 Rh7BG352200 Rh7BG436900 Rh7CG484200 Rh7DG452600
rosa_wichuraiana Rw4G009630 Rw4G009640 Rw4G009650 Rw4G009710 Rw7G038620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 221
AasI GACNNNNNNGTC 1 cut(s) 1017
Acc36I ACCTGC 2 cut(s) 172, 221
AccB1I GGYRCC 1 cut(s) 995
AciI CCGC 6 cut(s) 146, 315, 425, 960, 1169, 1305
AclWI GGATC 2 cut(s) 449, 698
AcoI YGGCCR 1 cut(s) 243
AcsI RAATTY 2 cut(s) 160, 780
AcuI CTGAAG 2 cut(s) 140, 795
AgsI TTSAA 8 cut(s) 32, 421, 440, 762, 893, 984, 1196, 1234
AhlI ACTAGT 1 cut(s) 601
AluBI AGCT 7 cut(s) 220, 276, 473, 662, 815, 889, 1310
AluI AGCT 7 cut(s) 220, 276, 473, 662, 815, 889, 1310
Alw21I GWGCWC 2 cut(s) 609, 1227
Alw44I GTGCAC 2 cut(s) 605, 1223
AlwI GGATC 2 cut(s) 449, 698
AlwNI CAGNNNCTG 3 cut(s) 236, 476, 1313
AoxI GGCC 3 cut(s) 143, 243, 390
ApaLI GTGCAC 2 cut(s) 605, 1223
ApoI RAATTY 2 cut(s) 160, 780
ArsI GACNNNNNNTTYG 2 cut(s) 1279, 1311
AspLEI GCGC 1 cut(s) 299
AspS9I GGNCC 1 cut(s) 143
AsuC2I CCSGG 2 cut(s) 1000, 1277
AsuHPI GGTGA 4 cut(s) 27, 85, 1210, 1277
AsuII TTCGAA 1 cut(s) 846
BaeGI GKGCMC 2 cut(s) 609, 1227
BalI TGGCCA 1 cut(s) 245
BanI GGYRCC 1 cut(s) 995
Bbv12I GWGCWC 2 cut(s) 609, 1227
BccI CCATC 4 cut(s) 686, 847, 1181, 1256
BciVI GTATCC 3 cut(s) 348, 1100, 1183
BclI TGATCA 3 cut(s) 822, 1134, 1240
BcnI CCSGG 2 cut(s) 1000, 1277
BcuI ACTAGT 1 cut(s) 601
BfaI CTAG 4 cut(s) 540, 602, 830, 1022
BfmI CTRYAG 2 cut(s) 468, 963
BfuAI ACCTGC 2 cut(s) 172, 221
BfuI GTATCC 3 cut(s) 348, 1100, 1183
Bme1390I CCNGG 2 cut(s) 1000, 1277
BmgT120I GGNCC 1 cut(s) 143
BmiI GGNNCC 2 cut(s) 570, 997
BmrFI CCNGG 2 cut(s) 1000, 1277
BmrI ACTGGG 2 cut(s) 1001, 1097
BmsI GCATC 1 cut(s) 266
BmuI ACTGGG 2 cut(s) 1001, 1097
Bpu14I TTCGAA 1 cut(s) 846
BpuMI CCSGG 2 cut(s) 1000, 1277
BsaJI CCNNGG 2 cut(s) 444, 646
BsaWI WCCGGW 1 cut(s) 866
BsaXI ACNNNNNCTCC 6 cut(s) 651, 681, 911, 941, 1294, 1324
Bse1I ACTGG 5 cut(s) 47, 271, 592, 996, 1092
Bse3DI GCAATG 2 cut(s) 378, 568
BseDI CCNNGG 2 cut(s) 444, 646
BseGI GGATG 1 cut(s) 1285
BseMI GCAATG 2 cut(s) 378, 568
BseMII CTCAG 2 cut(s) 912, 1238
BseNI ACTGG 5 cut(s) 47, 271, 592, 996, 1092
BseRI GAGGAG 1 cut(s) 672
BseSI GKGCMC 2 cut(s) 609, 1227
BshFI GGCC 3 cut(s) 145, 245, 392
BshNI GGYRCC 1 cut(s) 995
BsiHKAI GWGCWC 2 cut(s) 609, 1227
BsiSI CCGG 4 cut(s) 867, 999, 1276, 1298
BslFI GGGAC 1 cut(s) 99
BsmFI GGGAC 1 cut(s) 99
BsmI GAATGC 2 cut(s) 396, 953
BsnI GGCC 3 cut(s) 145, 245, 392
Bsp119I TTCGAA 1 cut(s) 846
Bsp1286I GDGCHC 2 cut(s) 609, 1227
Bsp143I GATC 5 cut(s) 454, 690, 822, 1134, 1240
BspACI CCGC 6 cut(s) 146, 315, 425, 960, 1169, 1305
BspANI GGCC 3 cut(s) 145, 245, 392
BspCNI CTCAG 2 cut(s) 911, 1239
BspLI GGNNCC 2 cut(s) 570, 997
BspMAI CTGCAG 2 cut(s) 472, 967
BspMI ACCTGC 2 cut(s) 172, 221
BspPI GGATC 2 cut(s) 449, 698
BspT104I TTCGAA 1 cut(s) 846
BspT107I GGYRCC 1 cut(s) 995
BsrDI GCAATG 2 cut(s) 378, 568
BsrI ACTGG 5 cut(s) 47, 271, 592, 996, 1092
BssECI CCNNGG 2 cut(s) 444, 646
BssMI GATC 5 cut(s) 454, 690, 822, 1134, 1240
BssT1I CCWWGG 2 cut(s) 444, 646
Bst4CI ACNGT 3 cut(s) 339, 1112, 1222
BstAPI GCANNNNNTGC 1 cut(s) 236
BstBI TTCGAA 1 cut(s) 846
BstC8I GCNNGC 5 cut(s) 317, 390, 394, 427, 970
BstDEI CTNAG 3 cut(s) 706, 898, 1247
BstF5I GGATG 1 cut(s) 1285
BstHHI GCGC 1 cut(s) 299
BstKTI GATC 5 cut(s) 457, 693, 825, 1137, 1243
BstMBI GATC 5 cut(s) 454, 690, 822, 1134, 1240
BstMWI GCNNNNNNNGC 8 cut(s) 236, 242, 473, 668, 959, 965, 1022, 1307
BstNSI RCATGY 2 cut(s) 68, 927
BstSCI CCNGG 2 cut(s) 998, 1275
BstSFI CTRYAG 2 cut(s) 468, 963
BstSLI GKGCMC 2 cut(s) 609, 1227
BstX2I RGATCY 1 cut(s) 454
BstYI RGATCY 1 cut(s) 454
BsuI GTATCC 3 cut(s) 348, 1100, 1183
BsuRI GGCC 3 cut(s) 145, 245, 392
BtsCI GGATG 1 cut(s) 1285
BtsI GCAGTG 2 cut(s) 1126, 1320
BtsIMutI CAGTG 4 cut(s) 585, 906, 1126, 1320
BveI ACCTGC 2 cut(s) 172, 221
Cac8I GCNNGC 5 cut(s) 317, 390, 394, 427, 970
CaiI CAGNNNCTG 3 cut(s) 236, 476, 1313
CfoI GCGC 1 cut(s) 299
Cfr13I GGNCC 1 cut(s) 143
CseI GACGC 1 cut(s) 1277
CspCI CAANNNNNGTGG 2 cut(s) 235, 270
CviAII CATG 7 cut(s) 65, 196, 747, 776, 924, 931, 940
DdeI CTNAG 3 cut(s) 706, 898, 1247
DpnI GATC 5 cut(s) 456, 692, 824, 1136, 1242
DpnII GATC 5 cut(s) 454, 690, 822, 1134, 1240
DrdI GACNNNNNNGTC 1 cut(s) 1017
DseDI GACNNNNNNGTC 1 cut(s) 1017
EaeI YGGCCR 1 cut(s) 243
Eco130I CCWWGG 2 cut(s) 444, 646
Eco57I CTGAAG 2 cut(s) 140, 795
EcoRI GAATTC 1 cut(s) 780
EcoT14I CCWWGG 2 cut(s) 444, 646
EcoT22I ATGCAT 1 cut(s) 1046
ErhI CCWWGG 2 cut(s) 444, 646
FaeI CATG 7 cut(s) 68, 199, 750, 779, 927, 934, 943
FaqI GGGAC 1 cut(s) 99
FatI CATG 7 cut(s) 64, 195, 746, 775, 923, 930, 939
FauI CCCGC 2 cut(s) 308, 418
FauNDI CATATG 2 cut(s) 325, 345
FbaI TGATCA 3 cut(s) 822, 1134, 1240
FokI GGATG 1 cut(s) 1292
FspBI CTAG 4 cut(s) 540, 602, 830, 1022
GlaI GCGC 1 cut(s) 298
HaeIII GGCC 3 cut(s) 145, 245, 392
HapII CCGG 4 cut(s) 867, 999, 1276, 1298
HgaI GACGC 1 cut(s) 1277
HhaI GCGC 1 cut(s) 299
Hin1II CATG 7 cut(s) 68, 199, 750, 779, 927, 934, 943
Hin6I GCGC 1 cut(s) 297
HinP1I GCGC 1 cut(s) 297
HinfI GANTC 4 cut(s) 32, 43, 543, 943
HpaII CCGG 4 cut(s) 867, 999, 1276, 1298
HphI GGTGA 4 cut(s) 27, 85, 1210, 1277
Hpy166II GTNNAC 3 cut(s) 607, 740, 1225
Hpy188I TCNGA 3 cut(s) 722, 1083, 1240
Hpy188III TCNNGA 6 cut(s) 29, 452, 458, 496, 540, 1313
Hpy8I GTNNAC 3 cut(s) 607, 740, 1225
HpyAV CCTTC 3 cut(s) 819, 1052, 1240
HpyCH4III ACNGT 3 cut(s) 339, 1112, 1222
HpyF10VI GCNNNNNNNGC 8 cut(s) 236, 242, 473, 668, 959, 965, 1022, 1307
HpyF3I CTNAG 3 cut(s) 706, 898, 1247
Hsp92II CATG 7 cut(s) 68, 199, 750, 779, 927, 934, 943
HspAI GCGC 1 cut(s) 297
Ksp22I TGATCA 3 cut(s) 822, 1134, 1240
Kzo9I GATC 5 cut(s) 454, 690, 822, 1134, 1240
LmnI GCTCC 5 cut(s) 217, 329, 659, 932, 1315
LweI GCATC 1 cut(s) 266
MaeI CTAG 4 cut(s) 540, 602, 830, 1022
MaeIII GTNAC 4 cut(s) 262, 1051, 1112, 1265
MalI GATC 5 cut(s) 456, 692, 824, 1136, 1242
MboI GATC 5 cut(s) 454, 690, 822, 1134, 1240
MboII GAAGA 5 cut(s) 23, 133, 374, 428, 866
MflI RGATCY 1 cut(s) 454
MhlI GDGCHC 2 cut(s) 609, 1227
MlsI TGGCCA 1 cut(s) 245
MluCI AATT 9 cut(s) 89, 104, 160, 462, 780, 797, 848, 1138, 1234
MluNI TGGCCA 1 cut(s) 245
MmeI TCCRAC 2 cut(s) 550, 1126
MnlI CCTC 6 cut(s) 360, 650, 742, 760, 907, 957
Mox20I TGGCCA 1 cut(s) 245
Mph1103I ATGCAT 1 cut(s) 1046
MscI TGGCCA 1 cut(s) 245
MseI TTAA 1 cut(s) 918
Msp20I TGGCCA 1 cut(s) 245
MspA1I CMGCKG 3 cut(s) 148, 473, 962
MspI CCGG 4 cut(s) 867, 999, 1276, 1298
MspR9I CCNGG 2 cut(s) 1000, 1277
Mva1269I GAATGC 2 cut(s) 396, 953
MwoI GCNNNNNNNGC 8 cut(s) 236, 242, 473, 668, 959, 965, 1022, 1307
NciI CCSGG 2 cut(s) 1000, 1277
NdeI CATATG 2 cut(s) 325, 345
NdeII GATC 5 cut(s) 454, 690, 822, 1134, 1240
NlaIII CATG 7 cut(s) 68, 199, 750, 779, 927, 934, 943
NlaIV GGNNCC 2 cut(s) 570, 997
NmuCI GTSAC 2 cut(s) 262, 1265
NsiI ATGCAT 1 cut(s) 1046
NspI RCATGY 2 cut(s) 68, 927
NspV TTCGAA 1 cut(s) 846
PaqCI CACCTGC 1 cut(s) 221
PcsI WCGNNNNNNNCGW 1 cut(s) 1015
PctI GAATGC 2 cut(s) 396, 953
PfeI GAWTC 4 cut(s) 32, 43, 543, 943
PflFI GACNNNGTC 1 cut(s) 1271
PfoI TCCNGGA 1 cut(s) 1275
PspN4I GGNNCC 2 cut(s) 570, 997
PspPI GGNCC 1 cut(s) 143
PstI CTGCAG 2 cut(s) 472, 967
PstNI CAGNNNCTG 3 cut(s) 236, 476, 1313
PsuI RGATCY 1 cut(s) 454
PsyI GACNNNGTC 1 cut(s) 1271
PvuII CAGCTG 1 cut(s) 473
SaqAI TTAA 1 cut(s) 918
Sau3AI GATC 5 cut(s) 454, 690, 822, 1134, 1240
Sau96I GGNCC 1 cut(s) 143
ScrFI CCNGG 2 cut(s) 1000, 1277
SduI GDGCHC 2 cut(s) 609, 1227
SfaNI GCATC 1 cut(s) 266
SfcI CTRYAG 2 cut(s) 468, 963
SfuI TTCGAA 1 cut(s) 846
SpeI ACTAGT 1 cut(s) 601
Sse9I AATT 9 cut(s) 89, 104, 160, 462, 780, 797, 848, 1138, 1234
SsiI CCGC 6 cut(s) 146, 315, 425, 960, 1169, 1305
SspMI CTAG 4 cut(s) 540, 602, 830, 1022
StyD4I CCNGG 2 cut(s) 998, 1275
StyI CCWWGG 2 cut(s) 444, 646
TaaI ACNGT 3 cut(s) 339, 1112, 1222
TaqI TCGA 2 cut(s) 846, 1093
TasI AATT 9 cut(s) 89, 104, 160, 462, 780, 797, 848, 1138, 1234
TauI GCSGC 2 cut(s) 148, 962
TfiI GAWTC 4 cut(s) 32, 43, 543, 943
Tru1I TTAA 1 cut(s) 918
Tru9I TTAA 1 cut(s) 918
TscAI CASTG 4 cut(s) 592, 906, 1126, 1327
TseFI GTSAC 2 cut(s) 262, 1265
Tsp45I GTSAC 2 cut(s) 262, 1265
TspDTI ATGAA 3 cut(s) 240, 764, 781
TspRI CASTG 4 cut(s) 592, 906, 1126, 1327
Tth111I GACNNNGTC 1 cut(s) 1271
VneI GTGCAC 2 cut(s) 605, 1223
XapI RAATTY 2 cut(s) 160, 780
XbaI TCTAGA 1 cut(s) 539
XceI RCATGY 2 cut(s) 68, 927
XcmI CCANNNNNNNNNTGG 1 cut(s) 1184
XspI CTAG 4 cut(s) 540, 602, 830, 1022
Zsp2I ATGCAT 1 cut(s) 1046
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.