Rh4AG118900

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000521
Physical Location & Seq
Reverse (-)
25656712 .. 25657842
1131 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG118900.1

Sequence Viewer

Length: 483 bp
ATGGTATACATGATTCCTCTTGCATTCTCCAGTGCAGCAAGCACACGAGTTTCAAATCAATTGGGTGCCGGGCAACCACGACTAGCTCGTCTAGCAGTACTTTGGGGCTACAGTTATAGTAGTGAAGAGGAAGTAGTGAACTATGTTGGACAAATGCTGATTTTGCTCGCGGTATCACACTTTTTTGATGGACTTCAATCTGTGCTTTCAGGTATCATAAGAGGAAGTGGACAGCAGAAGATCGGAGCATATGTTAACCTGGGAGCTTATTATCTTATGGGCATTCCTACTTCAATATTATTAGCTTTTGTATTTCACATTGGAGGAAAGGGTCTTTGGATGGGAATCATCGTTGCACTGTTTGTGCAAGCACTATTTCTGGGAATCATAATCTTATTCACAGATTGGGAGAAAGAAGTCAAGAAAGCTTTTGATAGAGTGTACAATACAATGAGCGTGGCTGATGCAACATCTATAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

160

Amino Acids

17.48

Weight (kDa)

7.97

Isoelectric Point (pI)

31.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 35 - 99 7.9e-09 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000521)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g09990 FvH4_4g09990 FvH4_4g09990 FvH4_4g09990 FvH4_7g32860 FvH4_7g32860 FvH4_7g32860 FvH4_7g32860
malus_domestica MD11G1148000.v1.1 MD13G1266200.v1.1 MD14G1231800.v1.1
prunus_persica Prupe.1G090100_v2.0.a1 Prupe.1G090100_v2.0.a1 Prupe.1G090100_v2.0.a1 Prupe.1G090100_v2.0.a1 Prupe.1G090100_v2.0.a1 Prupe.1G090300_v2.0.a1 Prupe.1G090300_v2.0.a1 Prupe.1G090300_v2.0.a1 Prupe.1G090300_v2.0.a1 Prupe.1G090300_v2.0.a1
pyrus_communis pycom13g23330
rosa_chinensis RchiOBHm_Chr4g0403401 RchiOBHm_Chr4g0403411 RchiOBHm_Chr4g0403421 RchiOBHm_Chr4g0403461 RchiOBHm_Chr4g0403631 RchiOBHm_Chr7g0223601 RchiOBHm_Chr7g0238161
rosa_laevigata RLG00000000937 RLG00000001957 RLG00000008965 RLG00000008975 RLG00000008976 RLG00000008978
rosa_multiflora Rmu_co8335743.1_g000001 Rmu_sc0005734.1_g000001 Rmu_sc0007727.1_g000011 Rmu_sc0011775.1_g000002 Rmu_sc0011775.1_g000005 Rmu_sc0028062.1_g000002
rosa_roxburghii Rroxscaffold_3G00223300 Rroxscaffold_5G00348050 Rroxscaffold_5G00348060 Rroxscaffold_5G00348120 Rroxscaffold_5G00348240
rosa_rugosa Rorug04G0042300 Rorug04G0042400 Rorug04G0042500 Rorug04G0042600.1 Rorug04G0042700 Rorug04G0042800 Rorug04G0042900 Rorug04G0043000 Rorug04G0043100 Rorug04G0043200 Rorug04G0043300 Rorug04G0043400 Rorug04G0044700 Rorug07G0310600 Rorug07G0310700 Rorug07G0310700
rosa_samantha Rh4AG118700 Rh4AG118800 Rh4AG118900 Rh4AG119000 Rh4BG111600 Rh4BG111800 Rh4BG111900 Rh4BG112900 Rh4CG126600 Rh4CG126900 Rh4CG127800 Rh4DG111300 Rh4DG111400 Rh4DG111500 Rh7AG466500 Rh7BG352200 Rh7BG436900 Rh7CG484200 Rh7DG452600
rosa_wichuraiana Rw4G009630 Rw4G009640 Rw4G009650 Rw4G009710 Rw7G038620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 87
AccB1I GGYRCC 1 cut(s) 65
AccI GTMKAC 1 cut(s) 6
AccII CGCG 1 cut(s) 170
AciI CCGC 1 cut(s) 170
AfaI GTAC 2 cut(s) 99, 443
AgsI TTSAA 3 cut(s) 54, 197, 294
AjnI CCWGG 1 cut(s) 258
AjuI GAANNNNNNNTTGG 2 cut(s) 319, 351
AluBI AGCT 4 cut(s) 86, 266, 305, 428
AluI AGCT 4 cut(s) 86, 266, 305, 428
ApeKI GCWGC 1 cut(s) 35
AsuC2I CCSGG 1 cut(s) 70
BanI GGYRCC 1 cut(s) 65
BauI CACGAG 1 cut(s) 45
BbvI GCAGC 1 cut(s) 47
BccI CCATC 2 cut(s) 182, 334
BciT130I CCWGG 1 cut(s) 260
BcnI CCSGG 1 cut(s) 70
BfaI CTAG 2 cut(s) 83, 92
BfmI CTRYAG 1 cut(s) 109
BisI GCNGC 1 cut(s) 36
BlsI GCNGC 1 cut(s) 37
BmcAI AGTACT 1 cut(s) 99
Bme1390I CCNGG 2 cut(s) 70, 260
BmiI GGNNCC 1 cut(s) 67
BmrFI CCNGG 2 cut(s) 70, 260
BmsI GCATC 1 cut(s) 454
BpmI CTGGAG 1 cut(s) 13
BpuMI CCSGG 1 cut(s) 70
BsaBI GATNNNNATC 1 cut(s) 344
BsaJI CCNNGG 1 cut(s) 259
BsaXI ACNNNNNCTCC 2 cut(s) 237, 267
Bse1I ACTGG 1 cut(s) 30
Bse8I GATNNNNATC 1 cut(s) 344
BseBI CCWGG 1 cut(s) 260
BseDI CCNNGG 1 cut(s) 259
BseGI GGATG 1 cut(s) 345
BseJI GATNNNNATC 1 cut(s) 344
BseNI ACTGG 1 cut(s) 30
BseXI GCAGC 1 cut(s) 47
BsgI GTGCAG 1 cut(s) 54
Bsh1236I CGCG 1 cut(s) 170
BshNI GGYRCC 1 cut(s) 65
BsiSI CCGG 1 cut(s) 69
BsmI GAATGC 2 cut(s) 23, 282
Bsp1407I TGTACA 1 cut(s) 441
Bsp143I GATC 1 cut(s) 240
BspACI CCGC 1 cut(s) 170
BspFNI CGCG 1 cut(s) 170
BspLI GGNNCC 1 cut(s) 67
BspT107I GGYRCC 1 cut(s) 65
BsrGI TGTACA 1 cut(s) 441
BsrI ACTGG 1 cut(s) 30
BssECI CCNNGG 1 cut(s) 259
BssMI GATC 1 cut(s) 240
BssNAI GTATAC 1 cut(s) 7
BssSI CACGAG 1 cut(s) 45
Bst1107I GTATAC 1 cut(s) 7
Bst2BI CACGAG 1 cut(s) 45
Bst2UI CCWGG 1 cut(s) 260
Bst4CI ACNGT 2 cut(s) 113, 360
Bst6I CTCTTC 1 cut(s) 120
BstAUI TGTACA 1 cut(s) 441
BstC8I GCNNGC 3 cut(s) 40, 168, 369
BstF5I GGATG 1 cut(s) 345
BstFNI CGCG 1 cut(s) 170
BstKTI GATC 1 cut(s) 243
BstMBI GATC 1 cut(s) 240
BstMWI GCNNNNNNNGC 2 cut(s) 92, 163
BstNI CCWGG 1 cut(s) 260
BstSCI CCNGG 2 cut(s) 68, 258
BstSFI CTRYAG 1 cut(s) 109
BstUI CGCG 1 cut(s) 170
BstV1I GCAGC 1 cut(s) 47
BstZ17I GTATAC 1 cut(s) 7
BtsCI GGATG 1 cut(s) 345
BtsIMutI CAGTG 2 cut(s) 37, 356
Cac8I GCNNGC 3 cut(s) 40, 168, 369
Csp6I GTAC 2 cut(s) 98, 442
CspCI CAANNNNNGTGG 2 cut(s) 438, 473
CviAII CATG 1 cut(s) 10
CviJI RGCY 6 cut(s) 86, 108, 266, 305, 428, 461
CviKI_1 RGCY 6 cut(s) 86, 108, 266, 305, 428, 461
CviQI GTAC 2 cut(s) 98, 442
DpnI GATC 1 cut(s) 242
DpnII GATC 1 cut(s) 240
DrdI GACNNNNNNGTC 1 cut(s) 87
DseDI GACNNNNNNGTC 1 cut(s) 87
Eam1104I CTCTTC 1 cut(s) 120
EarI CTCTTC 1 cut(s) 120
EcoRII CCWGG 1 cut(s) 258
FaeI CATG 1 cut(s) 13
FatI CATG 1 cut(s) 9
FauNDI CATATG 1 cut(s) 250
FblI GTMKAC 1 cut(s) 6
Fnu4HI GCNGC 1 cut(s) 36
FokI GGATG 1 cut(s) 352
Fsp4HI GCNGC 1 cut(s) 36
FspBI CTAG 2 cut(s) 83, 92
GluI GCNGC 1 cut(s) 36
GsuI CTGGAG 1 cut(s) 13
HapII CCGG 1 cut(s) 69
Hin1II CATG 1 cut(s) 13
HincII GTYRAC 1 cut(s) 256
HindII GTYRAC 1 cut(s) 256
HindIII AAGCTT 1 cut(s) 426
HinfI GANTC 3 cut(s) 13, 345, 384
HpaI GTTAAC 1 cut(s) 256
HpaII CCGG 1 cut(s) 69
Hpy166II GTNNAC 5 cut(s) 7, 139, 230, 256, 442
Hpy188I TCNGA 1 cut(s) 245
Hpy188III TCNNGA 1 cut(s) 421
Hpy8I GTNNAC 5 cut(s) 7, 139, 230, 256, 442
HpyCH4III ACNGT 2 cut(s) 113, 360
HpyCH4V TGCA 5 cut(s) 23, 35, 356, 367, 467
HpyF10VI GCNNNNNNNGC 2 cut(s) 92, 163
Hsp92II CATG 1 cut(s) 13
KspAI GTTAAC 1 cut(s) 256
Kzo9I GATC 1 cut(s) 240
LmnI GCTCC 2 cut(s) 245, 263
LpnPI CCDG 6 cut(s) 43, 82, 195, 245, 272, 365
Lsp1109I GCAGC 1 cut(s) 47
LweI GCATC 1 cut(s) 454
MaeI CTAG 2 cut(s) 83, 92
MalI GATC 1 cut(s) 242
MboI GATC 1 cut(s) 240
MboII GAAGA 2 cut(s) 137, 250
MfeI CAATTG 1 cut(s) 59
MluCI AATT 1 cut(s) 59
MmeI TCCRAC 1 cut(s) 127
MnlI CCTC 4 cut(s) 27, 121, 215, 317
MseI TTAA 1 cut(s) 255
MspI CCGG 1 cut(s) 69
MspR9I CCNGG 2 cut(s) 70, 260
MunI CAATTG 1 cut(s) 59
Mva1269I GAATGC 2 cut(s) 23, 282
MvaI CCWGG 1 cut(s) 260
MvnI CGCG 1 cut(s) 170
MwoI GCNNNNNNNGC 2 cut(s) 92, 163
NciI CCSGG 1 cut(s) 70
NdeI CATATG 1 cut(s) 250
NdeII GATC 1 cut(s) 240
NlaIII CATG 1 cut(s) 13
NlaIV GGNNCC 1 cut(s) 67
PcsI WCGNNNNNNNCGW 1 cut(s) 85
PctI GAATGC 2 cut(s) 23, 282
PfeI GAWTC 3 cut(s) 13, 345, 384
PkrI GCNGC 1 cut(s) 37
Psp6I CCWGG 1 cut(s) 258
PspGI CCWGG 1 cut(s) 258
PspN4I GGNNCC 1 cut(s) 67
RsaI GTAC 2 cut(s) 99, 443
RsaNI GTAC 2 cut(s) 98, 442
SaqAI TTAA 1 cut(s) 255
SatI GCNGC 1 cut(s) 36
Sau3AI GATC 1 cut(s) 240
ScaI AGTACT 1 cut(s) 99
ScrFI CCNGG 2 cut(s) 70, 260
SetI ASST 6 cut(s) 88, 214, 261, 268, 307, 430
SfaNI GCATC 1 cut(s) 454
SfcI CTRYAG 1 cut(s) 109
Sse9I AATT 1 cut(s) 59
SsiI CCGC 1 cut(s) 170
SspI AATATT 1 cut(s) 297
SspMI CTAG 2 cut(s) 83, 92
StyD4I CCNGG 2 cut(s) 68, 258
TaaI ACNGT 2 cut(s) 113, 360
TasI AATT 1 cut(s) 59
TatI WGTACW 2 cut(s) 97, 441
TfiI GAWTC 3 cut(s) 13, 345, 384
Tru1I TTAA 1 cut(s) 255
Tru9I TTAA 1 cut(s) 255
TscAI CASTG 2 cut(s) 37, 363
TseI GCWGC 1 cut(s) 35
TspRI CASTG 2 cut(s) 37, 363
XmiI GTMKAC 1 cut(s) 6
XspI CTAG 2 cut(s) 83, 92
ZrmI AGTACT 1 cut(s) 99
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.