Rh4CG127800

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0000521
Physical Location & Seq
Forward (+)
25114054 .. 25115837
1784 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG127800.1

Sequence Viewer

Length: 981 bp
ATGGCTGACGAAGAACAATCTGCTGGTCTTGAATCACCCTTGATTCCTGTTCTGCCCCAAGAACATGCATTACAATCAACAAAGGGACGATTCACTAAAGGCGAATTGTTCGGGGAACTGAAGAAGCAGTTATTGTTGGCAGGGCCGCTGGTATCATCAAATTTCTTGTTGTTCGGTATGCAGGTTATTTCAGTCATGTATGTTGGTCATCTTGGGGAGCTATCACTTGCAGGTGCTTCCATGGCCACTTCTTTTGCATCTGTGACTGGTTTGAGCTTGATAATAGGAATGGGTAGCGCATTAGACACATTTTGCGGGCAGTCATATGGAGCAAAACAGTATCATATGCTTGGTGTACACATGCAGAGGGCAATGCTTGTTCTTCTGCTGGCCTGCATTCCTCTTGCAACTATATGGTTCAATGCAGGCCACATTCTCAAATTCTTGGGTCAAGATCCAGAAATTGCCGCAGCTGCTGGAAACTATGCTCGTTTCCTGATACCTTGCATTTTTGCTTACGCAGTCCAACAATGTCATTCCAGATTCTTGCAAACCCAAAACAATGTGGTTCCTATGATAGCTACCACAGGTATTGCAACACTAGTGCACTTGCTAATCTGTTGGCTTCTCATATACAAGACCAGTTTGGGATATAGAGGTGCTGCTGTCGCGATCTCTATCTCATATTGGATCAATGCATTACTCTTGGTTGTTTATATCAGAGTTTCTCCTTCTTGTATACACACATGGACTGGATTCTCAAAGGAGGCATTTCATGGAATTCCCAATTTCATAAGACTATCTATTCCTTCAGCTATAATGATCAGCCTAGAAATCTGGTCATTCGAAATGATGGTTCTCTTATCCGGTTTTCTTCCAAATCCACAGCTTGAAACCTCAGTGCTATCAATCAGCCTTAACACATGCTCCATGTTATACATGATTCCTCTTGCATTCGCCGGTGCAGCAAGGTTTGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

326

Amino Acids

35.44

Weight (kDa)

7.65

Isoelectric Point (pI)

30.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 49 - 209 9.4e-40 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000521)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g09990 FvH4_4g09990 FvH4_4g09990 FvH4_4g09990 FvH4_7g32860 FvH4_7g32860 FvH4_7g32860 FvH4_7g32860
malus_domestica MD11G1148000.v1.1 MD13G1266200.v1.1 MD14G1231800.v1.1
prunus_persica Prupe.1G090100_v2.0.a1 Prupe.1G090100_v2.0.a1 Prupe.1G090100_v2.0.a1 Prupe.1G090100_v2.0.a1 Prupe.1G090100_v2.0.a1 Prupe.1G090300_v2.0.a1 Prupe.1G090300_v2.0.a1 Prupe.1G090300_v2.0.a1 Prupe.1G090300_v2.0.a1 Prupe.1G090300_v2.0.a1
pyrus_communis pycom13g23330
rosa_chinensis RchiOBHm_Chr4g0403401 RchiOBHm_Chr4g0403411 RchiOBHm_Chr4g0403421 RchiOBHm_Chr4g0403461 RchiOBHm_Chr4g0403631 RchiOBHm_Chr7g0223601 RchiOBHm_Chr7g0238161
rosa_laevigata RLG00000000937 RLG00000001957 RLG00000008965 RLG00000008975 RLG00000008976 RLG00000008978
rosa_multiflora Rmu_co8335743.1_g000001 Rmu_sc0005734.1_g000001 Rmu_sc0007727.1_g000011 Rmu_sc0011775.1_g000002 Rmu_sc0011775.1_g000005 Rmu_sc0028062.1_g000002
rosa_roxburghii Rroxscaffold_3G00223300 Rroxscaffold_5G00348050 Rroxscaffold_5G00348060 Rroxscaffold_5G00348120 Rroxscaffold_5G00348240
rosa_rugosa Rorug04G0042300 Rorug04G0042400 Rorug04G0042500 Rorug04G0042600.1 Rorug04G0042700 Rorug04G0042800 Rorug04G0042900 Rorug04G0043000 Rorug04G0043100 Rorug04G0043200 Rorug04G0043300 Rorug04G0043400 Rorug04G0044700 Rorug07G0310600 Rorug07G0310700 Rorug07G0310700
rosa_samantha Rh4AG118700 Rh4AG118800 Rh4AG118900 Rh4AG119000 Rh4BG111600 Rh4BG111800 Rh4BG111900 Rh4BG112900 Rh4CG126600 Rh4CG126900 Rh4CG127800 Rh4DG111300 Rh4DG111400 Rh4DG111500 Rh7AG466500 Rh7BG352200 Rh7BG436900 Rh7CG484200 Rh7DG452600
rosa_wichuraiana Rw4G009630 Rw4G009640 Rw4G009650 Rw4G009710 Rw7G038620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 221
Acc36I ACCTGC 2 cut(s) 172, 221
AccI GTMKAC 1 cut(s) 739
AccII CGCG 1 cut(s) 671
AciI CCGC 3 cut(s) 146, 315, 468
AclWI GGATC 2 cut(s) 449, 698
AcoI YGGCCR 1 cut(s) 243
AcsI RAATTY 3 cut(s) 160, 440, 780
AcuI CTGAAG 2 cut(s) 140, 795
AfaI GTAC 1 cut(s) 357
AgsI TTSAA 3 cut(s) 32, 421, 893
AhlI ACTAGT 1 cut(s) 601
AluBI AGCT 6 cut(s) 220, 276, 473, 581, 815, 889
AluI AGCT 6 cut(s) 220, 276, 473, 581, 815, 889
Alw21I GWGCWC 1 cut(s) 609
Alw44I GTGCAC 1 cut(s) 605
AlwI GGATC 2 cut(s) 449, 698
AlwNI CAGNNNCTG 1 cut(s) 476
AoxI GGCC 4 cut(s) 143, 243, 390, 427
ApaLI GTGCAC 1 cut(s) 605
ApeKI GCWGC 4 cut(s) 470, 473, 662, 965
ApoI RAATTY 3 cut(s) 160, 440, 780
AspLEI GCGC 1 cut(s) 299
AspS9I GGNCC 1 cut(s) 143
AsuHPI GGTGA 1 cut(s) 27
AsuII TTCGAA 1 cut(s) 846
BaeGI GKGCMC 1 cut(s) 609
BalI TGGCCA 1 cut(s) 245
Bbv12I GWGCWC 1 cut(s) 609
BbvI GCAGC 4 cut(s) 460, 482, 649, 977
BccI CCATC 1 cut(s) 847
BclI TGATCA 1 cut(s) 822
BcuI ACTAGT 1 cut(s) 601
BfaI CTAG 2 cut(s) 602, 830
BfuAI ACCTGC 2 cut(s) 172, 221
BisI GCNGC 6 cut(s) 146, 468, 471, 474, 663, 966
BlsI GCNGC 6 cut(s) 147, 469, 472, 475, 664, 967
BmgT120I GGNCC 1 cut(s) 143
BmiI GGNNCC 1 cut(s) 570
BmsI GCATC 1 cut(s) 266
Bpu14I TTCGAA 1 cut(s) 846
BsaBI GATNNNNATC 1 cut(s) 677
BsaJI CCNNGG 1 cut(s) 240
BsaWI WCCGGW 1 cut(s) 866
BsaXI ACNNNNNCTCC 2 cut(s) 911, 941
Bse118I RCCGGY 1 cut(s) 959
Bse1I ACTGG 3 cut(s) 271, 642, 757
Bse3DI GCAATG 1 cut(s) 378
Bse8I GATNNNNATC 1 cut(s) 677
BseDI CCNNGG 1 cut(s) 240
BseJI GATNNNNATC 1 cut(s) 677
BseMI GCAATG 1 cut(s) 378
BseMII CTCAG 1 cut(s) 912
BseNI ACTGG 3 cut(s) 271, 642, 757
BseSI GKGCMC 1 cut(s) 609
BseXI GCAGC 4 cut(s) 460, 482, 649, 977
Bsh1236I CGCG 1 cut(s) 671
BshFI GGCC 4 cut(s) 145, 245, 392, 429
BsiHKAI GWGCWC 1 cut(s) 609
BsiSI CCGG 2 cut(s) 867, 960
BslFI GGGAC 1 cut(s) 99
BsmFI GGGAC 1 cut(s) 99
BsmI GAATGC 2 cut(s) 396, 953
BsnI GGCC 4 cut(s) 145, 245, 392, 429
Bsp119I TTCGAA 1 cut(s) 846
Bsp1286I GDGCHC 1 cut(s) 609
Bsp1407I TGTACA 1 cut(s) 355
Bsp143I GATC 4 cut(s) 454, 672, 690, 822
Bsp19I CCATGG 1 cut(s) 240
Bsp68I TCGCGA 1 cut(s) 671
BspACI CCGC 3 cut(s) 146, 315, 468
BspANI GGCC 4 cut(s) 145, 245, 392, 429
BspCNI CTCAG 1 cut(s) 911
BspFNI CGCG 1 cut(s) 671
BspLI GGNNCC 1 cut(s) 570
BspMI ACCTGC 2 cut(s) 172, 221
BspPI GGATC 2 cut(s) 449, 698
BspT104I TTCGAA 1 cut(s) 846
BsrDI GCAATG 1 cut(s) 378
BsrFI RCCGGY 1 cut(s) 959
BsrGI TGTACA 1 cut(s) 355
BsrI ACTGG 3 cut(s) 271, 642, 757
BssAI RCCGGY 1 cut(s) 959
BssECI CCNNGG 1 cut(s) 240
BssMI GATC 4 cut(s) 454, 672, 690, 822
BssNAI GTATAC 1 cut(s) 740
BssT1I CCWWGG 1 cut(s) 240
Bst1107I GTATAC 1 cut(s) 740
Bst4CI ACNGT 1 cut(s) 339
BstAUI TGTACA 1 cut(s) 355
BstBI TTCGAA 1 cut(s) 846
BstC8I GCNNGC 4 cut(s) 317, 390, 394, 427
BstDEI CTNAG 1 cut(s) 898
BstDSI CCRYGG 1 cut(s) 240
BstFNI CGCG 1 cut(s) 671
BstHHI GCGC 1 cut(s) 299
BstKTI GATC 4 cut(s) 457, 675, 693, 825
BstMBI GATC 4 cut(s) 454, 672, 690, 822
BstMWI GCNNNNNNNGC 4 cut(s) 242, 473, 668, 965
BstNSI RCATGY 3 cut(s) 68, 364, 927
BstSLI GKGCMC 1 cut(s) 609
BstUI CGCG 1 cut(s) 671
BstV1I GCAGC 4 cut(s) 460, 482, 649, 977
BstX2I RGATCY 1 cut(s) 454
BstYI RGATCY 1 cut(s) 454
BstZ17I GTATAC 1 cut(s) 740
BsuRI GGCC 4 cut(s) 145, 245, 392, 429
BtgI CCRYGG 1 cut(s) 240
BtsIMutI CAGTG 1 cut(s) 906
BtuMI TCGCGA 1 cut(s) 671
BveI ACCTGC 2 cut(s) 172, 221
Cac8I GCNNGC 4 cut(s) 317, 390, 394, 427
CaiI CAGNNNCTG 1 cut(s) 476
CfoI GCGC 1 cut(s) 299
Cfr10I RCCGGY 1 cut(s) 959
Cfr13I GGNCC 1 cut(s) 143
Csp6I GTAC 1 cut(s) 356
CspCI CAANNNNNGTGG 4 cut(s) 235, 270, 574, 609
CviAII CATG 9 cut(s) 65, 196, 241, 361, 747, 776, 924, 931, 940
CviQI GTAC 1 cut(s) 356
DdeI CTNAG 1 cut(s) 898
DpnI GATC 4 cut(s) 456, 674, 692, 824
DpnII GATC 4 cut(s) 454, 672, 690, 822
EaeI YGGCCR 1 cut(s) 243
Eco130I CCWWGG 1 cut(s) 240
Eco57I CTGAAG 2 cut(s) 140, 795
EcoRI GAATTC 1 cut(s) 780
EcoT14I CCWWGG 1 cut(s) 240
EcoT22I ATGCAT 2 cut(s) 70, 700
ErhI CCWWGG 1 cut(s) 240
FaeI CATG 9 cut(s) 68, 199, 244, 364, 750, 779, 927, 934, 943
FaqI GGGAC 1 cut(s) 99
FatI CATG 9 cut(s) 64, 195, 240, 360, 746, 775, 923, 930, 939
FauI CCCGC 1 cut(s) 308
FauNDI CATATG 2 cut(s) 325, 345
FbaI TGATCA 1 cut(s) 822
FblI GTMKAC 1 cut(s) 739
Fnu4HI GCNGC 6 cut(s) 146, 468, 471, 474, 663, 966
Fsp4HI GCNGC 6 cut(s) 146, 468, 471, 474, 663, 966
FspBI CTAG 2 cut(s) 602, 830
GlaI GCGC 1 cut(s) 298
GluI GCNGC 6 cut(s) 146, 468, 471, 474, 663, 966
HaeIII GGCC 4 cut(s) 145, 245, 392, 429
HapII CCGG 2 cut(s) 867, 960
HhaI GCGC 1 cut(s) 299
Hin1II CATG 9 cut(s) 68, 199, 244, 364, 750, 779, 927, 934, 943
Hin6I GCGC 1 cut(s) 297
HinP1I GCGC 1 cut(s) 297
HinfI GANTC 6 cut(s) 32, 43, 90, 543, 756, 943
HpaII CCGG 2 cut(s) 867, 960
HphI GGTGA 1 cut(s) 27
Hpy166II GTNNAC 4 cut(s) 356, 358, 607, 740
Hpy188I TCNGA 1 cut(s) 722
Hpy188III TCNNGA 6 cut(s) 29, 452, 458, 496, 540, 670
Hpy8I GTNNAC 4 cut(s) 356, 358, 607, 740
HpyAV CCTTC 2 cut(s) 741, 819
HpyCH4III ACNGT 1 cut(s) 339
HpyF10VI GCNNNNNNNGC 4 cut(s) 242, 473, 668, 965
HpyF3I CTNAG 1 cut(s) 898
Hsp92II CATG 9 cut(s) 68, 199, 244, 364, 750, 779, 927, 934, 943
HspAI GCGC 1 cut(s) 297
Ksp22I TGATCA 1 cut(s) 822
Kzo9I GATC 4 cut(s) 454, 672, 690, 822
LmnI GCTCC 3 cut(s) 217, 329, 932
Lsp1109I GCAGC 4 cut(s) 460, 482, 649, 977
LweI GCATC 1 cut(s) 266
MaeI CTAG 2 cut(s) 602, 830
MaeIII GTNAC 1 cut(s) 262
MalI GATC 4 cut(s) 456, 674, 692, 824
MboI GATC 4 cut(s) 454, 672, 690, 822
MboII GAAGA 4 cut(s) 23, 133, 374, 866
MflI RGATCY 1 cut(s) 454
MhlI GDGCHC 1 cut(s) 609
MlsI TGGCCA 1 cut(s) 245
MluCI AATT 6 cut(s) 104, 160, 440, 462, 780, 787
MluNI TGGCCA 1 cut(s) 245
MmeI TCCRAC 1 cut(s) 550
MnlI CCTC 6 cut(s) 360, 411, 650, 760, 907, 957
Mox20I TGGCCA 1 cut(s) 245
Mph1103I ATGCAT 2 cut(s) 70, 700
MscI TGGCCA 1 cut(s) 245
MseI TTAA 1 cut(s) 918
Msp20I TGGCCA 1 cut(s) 245
MspA1I CMGCKG 2 cut(s) 148, 473
MspI CCGG 2 cut(s) 867, 960
Mva1269I GAATGC 2 cut(s) 396, 953
MvnI CGCG 1 cut(s) 671
MwoI GCNNNNNNNGC 4 cut(s) 242, 473, 668, 965
NcoI CCATGG 1 cut(s) 240
NdeI CATATG 2 cut(s) 325, 345
NdeII GATC 4 cut(s) 454, 672, 690, 822
NlaIII CATG 9 cut(s) 68, 199, 244, 364, 750, 779, 927, 934, 943
NlaIV GGNNCC 1 cut(s) 570
NmuCI GTSAC 1 cut(s) 262
NruI TCGCGA 1 cut(s) 671
NsiI ATGCAT 2 cut(s) 70, 700
NspI RCATGY 3 cut(s) 68, 364, 927
NspV TTCGAA 1 cut(s) 846
PaqCI CACCTGC 1 cut(s) 221
PctI GAATGC 2 cut(s) 396, 953
PfeI GAWTC 6 cut(s) 32, 43, 90, 543, 756, 943
PkrI GCNGC 6 cut(s) 147, 469, 472, 475, 664, 967
PspN4I GGNNCC 1 cut(s) 570
PspPI GGNCC 1 cut(s) 143
PsrI GAACNNNNNNTAC 2 cut(s) 54, 86
PstNI CAGNNNCTG 1 cut(s) 476
PsuI RGATCY 1 cut(s) 454
PvuII CAGCTG 1 cut(s) 473
RruI TCGCGA 1 cut(s) 671
RsaI GTAC 1 cut(s) 357
RsaNI GTAC 1 cut(s) 356
SaqAI TTAA 1 cut(s) 918
SatI GCNGC 6 cut(s) 146, 468, 471, 474, 663, 966
Sau3AI GATC 4 cut(s) 454, 672, 690, 822
Sau96I GGNCC 1 cut(s) 143
SduI GDGCHC 1 cut(s) 609
SfaNI GCATC 1 cut(s) 266
SfuI TTCGAA 1 cut(s) 846
SgrAI CRCCGGYG 1 cut(s) 959
SpeI ACTAGT 1 cut(s) 601
Sse9I AATT 6 cut(s) 104, 160, 440, 462, 780, 787
SsiI CCGC 3 cut(s) 146, 315, 468
SspMI CTAG 2 cut(s) 602, 830
StyI CCWWGG 1 cut(s) 240
TaaI ACNGT 1 cut(s) 339
TaqI TCGA 1 cut(s) 846
TasI AATT 6 cut(s) 104, 160, 440, 462, 780, 787
TatI WGTACW 1 cut(s) 355
TauI GCSGC 2 cut(s) 148, 470
TfiI GAWTC 6 cut(s) 32, 43, 90, 543, 756, 943
Tru1I TTAA 1 cut(s) 918
Tru9I TTAA 1 cut(s) 918
TscAI CASTG 1 cut(s) 906
TseFI GTSAC 1 cut(s) 262
TseI GCWGC 4 cut(s) 470, 473, 662, 965
Tsp45I GTSAC 1 cut(s) 262
TspDTI ATGAA 2 cut(s) 764, 781
TspRI CASTG 1 cut(s) 906
VneI GTGCAC 1 cut(s) 605
XapI RAATTY 3 cut(s) 160, 440, 780
XceI RCATGY 3 cut(s) 68, 364, 927
XmiI GTMKAC 1 cut(s) 739
XspI CTAG 2 cut(s) 602, 830
Zsp2I ATGCAT 2 cut(s) 70, 700
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.