RLG00000008976

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0000521
Physical Location & Seq
Forward (+)
44189384 .. 44192085
2702 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008976

Sequence Viewer

Length: 1482 bp
ATGGCTGACGAAGAACAATCTGCAGATCTCGAATCACCCCTGATACCGGTGGTACTGCCCCAAGAACATGCATTACATTCAACCAAGCAACTTACAAAAGACGGATTTCTCGGGGAACTGAAGCAGCAGCTATTGCTGGCAGGGCCGCTTGTATCTTCAAATTTCTTGCTATGTGGTATGCAGGCTATTTCAGTCATGTATGTTGGTCATCTTGGGGAACTATCACTCGCAGCTGCTTCTACGGCAACTTCTTTTGCATCAGTCACTGGTTTGAGCTTGATTATAGGAATGGGTAGCGCATTGGACACATTTTGTGGGCAGTCCTATGGGGCAAAACAGTATCATATGCTTGGTGTACACATGCAGAGGGCAATGCTTGTTATTCTCTTGGCATGCCTTCCTCTTGCAACTATATGGTTCAATGCAGGCCACATTCTCAAATTCTTGGGTCAAGATCCAGAAATTGCCGCAGCCGCTGGAACTTATGCTCGTTTCCTGACACCATGCATTTTCGCTTATGCAATCCAACAATGTCATTCCAGATTCTTGCAAACCCAAAACAATGTGGTTCCCATGATAGTTACCACAGGTAGTGCAACACTAGTGCACTTGCTTCTCTGCTGGCTTCTTGTATATAAGACCAGCTTGGGATATAGAGGCGCTGCTGTCGCGATCTCTGTCTCATATTGGATCAATGCATTACTCTTGGTTGTTTATATCAGAGTTTCTCCTTCTTGTATACACACATGGACTGGATTCTCAAAGGAGGCATTTCATGGAATTCCCAATTTCATAAGACTCTCTATTCCTTCAGCTATAATGATCAGCTTAGAAATCTGGTCATTCGAAATGATGGTTCTCTTAGCTGGTTTCCTTCCAAATCCACGGCTTGAAACCTCAGTCCTATCAATCAGCCTTAATACATGTTCAATGGTATACATGATTCCTCTTGCATTCTCCAGTGCAGCAAGCACACGAGTTTCAAATCAATTGGGTGCCGGGCAACCACGACTAGCTCATCTAGCAGTAGGTGTTTCACTATGCATTGTTGTTGCTGAAGGTGTTGTGGTTGCTGCTGTACTGATATTGGGTCGAACAGTTTGGGGCTACTGTTATAGTAGTGAAGAGGAAGTAGTGAACTATGTTGGACAAATGCTGATTTTGCTCGCGGTATCACACTTTTTTGATGGACTTCAATCTGTGCTTTCAGGTATCATAAGAGGAAGTGGACAGCAGAAGCTCGGAGCATATGTTAACCTGGGAGCTTATTATCTTATGGGCATTCCTACTTCAGTATTATTAGCTTTTGTATTTCACACTGGAGGAAAGGGTCTTTGGCTGGGAATCATCGTGGCACTATTTGTGCAAGCACTATTTCTGGGAATCATAATCTTATTCACAGATTGGGAGAAAGAAGCGAAGAAAGCTTCTGATAGAGTGTACAATACAATGAGCGTGGCTCATGCAACATCTATAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

494

Amino Acids

53.16

Weight (kDa)

6.91

Isoelectric Point (pI)

27.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 49 - 209 1.5e-38 MatE
MatE PF01554 270 - 432 3.5e-26 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000521)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g09990 FvH4_4g09990 FvH4_4g09990 FvH4_4g09990 FvH4_7g32860 FvH4_7g32860 FvH4_7g32860 FvH4_7g32860
malus_domestica MD11G1148000.v1.1 MD13G1266200.v1.1 MD14G1231800.v1.1
prunus_persica Prupe.1G090100_v2.0.a1 Prupe.1G090100_v2.0.a1 Prupe.1G090100_v2.0.a1 Prupe.1G090100_v2.0.a1 Prupe.1G090100_v2.0.a1 Prupe.1G090300_v2.0.a1 Prupe.1G090300_v2.0.a1 Prupe.1G090300_v2.0.a1 Prupe.1G090300_v2.0.a1 Prupe.1G090300_v2.0.a1
pyrus_communis pycom13g23330
rosa_chinensis RchiOBHm_Chr4g0403401 RchiOBHm_Chr4g0403411 RchiOBHm_Chr4g0403421 RchiOBHm_Chr4g0403461 RchiOBHm_Chr4g0403631 RchiOBHm_Chr7g0223601 RchiOBHm_Chr7g0238161
rosa_laevigata RLG00000000937 RLG00000001957 RLG00000008965 RLG00000008975 RLG00000008976 RLG00000008978
rosa_multiflora Rmu_co8335743.1_g000001 Rmu_sc0005734.1_g000001 Rmu_sc0007727.1_g000011 Rmu_sc0011775.1_g000002 Rmu_sc0011775.1_g000005 Rmu_sc0028062.1_g000002
rosa_roxburghii Rroxscaffold_3G00223300 Rroxscaffold_5G00348050 Rroxscaffold_5G00348060 Rroxscaffold_5G00348120 Rroxscaffold_5G00348240
rosa_rugosa Rorug04G0042300 Rorug04G0042400 Rorug04G0042500 Rorug04G0042600.1 Rorug04G0042700 Rorug04G0042800 Rorug04G0042900 Rorug04G0043000 Rorug04G0043100 Rorug04G0043200 Rorug04G0043300 Rorug04G0043400 Rorug04G0044700 Rorug07G0310600 Rorug07G0310700 Rorug07G0310700
rosa_samantha Rh4AG118700 Rh4AG118800 Rh4AG118900 Rh4AG119000 Rh4BG111600 Rh4BG111800 Rh4BG111900 Rh4BG112900 Rh4CG126600 Rh4CG126900 Rh4CG127800 Rh4DG111300 Rh4DG111400 Rh4DG111500 Rh7AG466500 Rh7BG352200 Rh7BG436900 Rh7CG484200 Rh7DG452600
rosa_wichuraiana Rw4G009630 Rw4G009640 Rw4G009650 Rw4G009710 Rw7G038620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 995
AccI GTMKAC 2 cut(s) 739, 936
AccII CGCG 2 cut(s) 671, 1169
AciI CCGC 4 cut(s) 146, 468, 474, 1169
AclWI GGATC 2 cut(s) 449, 698
AcsI RAATTY 3 cut(s) 160, 440, 780
AcuI CTGAAG 4 cut(s) 140, 795, 1077, 1275
AfaI GTAC 4 cut(s) 54, 357, 1080, 1442
AfiI CCNNNNNNNGG 1 cut(s) 46
AflIII ACRYGT 1 cut(s) 923
AgeI ACCGGT 1 cut(s) 46
AgsI TTSAA 7 cut(s) 81, 159, 421, 893, 930, 984, 1196
AhlI ACTAGT 1 cut(s) 601
AjnI CCWGG 1 cut(s) 1257
AjuI GAANNNNNNNTTGG 2 cut(s) 1318, 1350
Alw21I GWGCWC 1 cut(s) 609
Alw26I GTCTC 1 cut(s) 685
Alw44I GTGCAC 1 cut(s) 605
AlwI GGATC 2 cut(s) 449, 698
AlwNI CAGNNNCTG 2 cut(s) 266, 476
Ama87I CYCGRG 1 cut(s) 110
AoxI GGCC 2 cut(s) 143, 427
ApaLI GTGCAC 1 cut(s) 605
ApeKI GCWGC 8 cut(s) 124, 127, 230, 233, 470, 662, 965, 1073
ApoI RAATTY 3 cut(s) 160, 440, 780
AsiGI ACCGGT 1 cut(s) 46
AspLEI GCGC 2 cut(s) 299, 662
AspS9I GGNCC 1 cut(s) 143
AsuC2I CCSGG 1 cut(s) 1000
AsuHPI GGTGA 1 cut(s) 27
AsuII TTCGAA 1 cut(s) 846
AvaI CYCGRG 1 cut(s) 110
BaeGI GKGCMC 1 cut(s) 609
BanI GGYRCC 1 cut(s) 995
BauI CACGAG 1 cut(s) 975
Bbv12I GWGCWC 1 cut(s) 609
BbvI GCAGC 8 cut(s) 136, 139, 220, 242, 482, 649, 977, 1060
BccI CCATC 2 cut(s) 847, 1181
BceAI ACGGC 2 cut(s) 258, 902
BciT130I CCWGG 1 cut(s) 1259
BclI TGATCA 1 cut(s) 822
BcnI CCSGG 1 cut(s) 1000
BcoDI GTCTC 1 cut(s) 685
BcuI ACTAGT 1 cut(s) 601
BfaI CTAG 3 cut(s) 602, 1013, 1022
BfmI CTRYAG 1 cut(s) 21
BfoI RGCGCY 1 cut(s) 663
BglII AGATCT 1 cut(s) 25
Bme1390I CCNGG 2 cut(s) 1000, 1259
BmeT110I CYCGRG 1 cut(s) 110
BmgT120I GGNCC 1 cut(s) 143
BmiI GGNNCC 2 cut(s) 570, 997
BmrFI CCNGG 2 cut(s) 1000, 1259
BmsI GCATC 1 cut(s) 266
BplI GAGNNNNNCTC 2 cut(s) 1444, 1476
BpmI CTGGAG 2 cut(s) 943, 1341
Bpu14I TTCGAA 1 cut(s) 846
BpuMI CCSGG 1 cut(s) 1000
BsaJI CCNNGG 2 cut(s) 884, 1258
BsaWI WCCGGW 1 cut(s) 46
BsaXI ACNNNNNCTCC 2 cut(s) 1236, 1266
Bsc4I CCNNNNNNNGG 1 cut(s) 46
Bse118I RCCGGY 1 cut(s) 46
Bse1I ACTGG 4 cut(s) 271, 757, 960, 1324
Bse3DI GCAATG 1 cut(s) 378
BseBI CCWGG 1 cut(s) 1259
BseDI CCNNGG 2 cut(s) 884, 1258
BseLI CCNNNNNNNGG 1 cut(s) 46
BseMI GCAATG 1 cut(s) 378
BseMII CTCAG 1 cut(s) 912
BseNI ACTGG 4 cut(s) 271, 757, 960, 1324
BseSI GKGCMC 1 cut(s) 609
BseXI GCAGC 8 cut(s) 136, 139, 220, 242, 482, 649, 977, 1060
BseYI CCCAGC 1 cut(s) 1339
BsgI GTGCAG 1 cut(s) 984
Bsh1236I CGCG 2 cut(s) 671, 1169
BshFI GGCC 2 cut(s) 145, 429
BshNI GGYRCC 1 cut(s) 995
BshTI ACCGGT 1 cut(s) 46
BsiHKAI GWGCWC 1 cut(s) 609
BsiHKCI CYCGRG 1 cut(s) 110
BsiSI CCGG 2 cut(s) 47, 999
BslI CCNNNNNNNGG 1 cut(s) 46
BsmAI GTCTC 1 cut(s) 685
BsmI GAATGC 2 cut(s) 953, 1281
BsnI GGCC 2 cut(s) 145, 429
BsoBI CYCGRG 1 cut(s) 110
Bsp119I TTCGAA 1 cut(s) 846
Bsp1286I GDGCHC 1 cut(s) 609
Bsp1407I TGTACA 2 cut(s) 355, 1440
Bsp143I GATC 5 cut(s) 25, 454, 672, 690, 822
Bsp68I TCGCGA 1 cut(s) 671
BspACI CCGC 4 cut(s) 146, 468, 474, 1169
BspANI GGCC 2 cut(s) 145, 429
BspCNI CTCAG 1 cut(s) 911
BspFNI CGCG 2 cut(s) 671, 1169
BspLI GGNNCC 2 cut(s) 570, 997
BspMAI CTGCAG 1 cut(s) 25
BspPI GGATC 2 cut(s) 449, 698
BspT104I TTCGAA 1 cut(s) 846
BspT107I GGYRCC 1 cut(s) 995
BsrDI GCAATG 1 cut(s) 378
BsrFI RCCGGY 1 cut(s) 46
BsrGI TGTACA 2 cut(s) 355, 1440
BsrI ACTGG 4 cut(s) 271, 757, 960, 1324
BssAI RCCGGY 1 cut(s) 46
BssECI CCNNGG 2 cut(s) 884, 1258
BssMI GATC 5 cut(s) 25, 454, 672, 690, 822
BssNAI GTATAC 2 cut(s) 740, 937
BssSI CACGAG 1 cut(s) 975
Bst1107I GTATAC 2 cut(s) 740, 937
Bst2BI CACGAG 1 cut(s) 975
Bst2UI CCWGG 1 cut(s) 1259
Bst4CI ACNGT 3 cut(s) 339, 1099, 1112
Bst6I CTCTTC 1 cut(s) 1119
BstAPI GCANNNNNTGC 1 cut(s) 133
BstAUI TGTACA 2 cut(s) 355, 1440
BstBI TTCGAA 1 cut(s) 846
BstC8I GCNNGC 8 cut(s) 138, 183, 394, 427, 623, 970, 1167, 1368
BstDEI CTNAG 3 cut(s) 829, 862, 898
BstDSI CCRYGG 1 cut(s) 884
BstFNI CGCG 2 cut(s) 671, 1169
BstH2I RGCGCY 1 cut(s) 663
BstHHI GCGC 2 cut(s) 299, 662
BstKTI GATC 5 cut(s) 28, 457, 675, 693, 825
BstMAI GTCTC 1 cut(s) 685
BstMBI GATC 5 cut(s) 25, 454, 672, 690, 822
BstMWI GCNNNNNNNGC 8 cut(s) 133, 142, 242, 473, 668, 1022, 1162, 1424
BstNI CCWGG 1 cut(s) 1259
BstNSI RCATGY 4 cut(s) 71, 364, 396, 927
BstSCI CCNGG 2 cut(s) 998, 1257
BstSFI CTRYAG 1 cut(s) 21
BstSLI GKGCMC 1 cut(s) 609
BstUI CGCG 2 cut(s) 671, 1169
BstV1I GCAGC 8 cut(s) 136, 139, 220, 242, 482, 649, 977, 1060
BstX2I RGATCY 2 cut(s) 25, 454
BstYI RGATCY 2 cut(s) 25, 454
BstZ17I GTATAC 2 cut(s) 740, 937
BsuRI GGCC 2 cut(s) 145, 429
BtgI CCRYGG 1 cut(s) 884
BtsIMutI CAGTG 3 cut(s) 264, 967, 1317
BtuMI TCGCGA 1 cut(s) 671
Cac8I GCNNGC 8 cut(s) 138, 183, 394, 427, 623, 970, 1167, 1368
CaiI CAGNNNCTG 2 cut(s) 266, 476
CfoI GCGC 2 cut(s) 299, 662
Cfr10I RCCGGY 1 cut(s) 46
Cfr13I GGNCC 1 cut(s) 143
Csp6I GTAC 4 cut(s) 53, 356, 1079, 1441
CspAI ACCGGT 1 cut(s) 46
CspCI CAANNNNNGTGG 2 cut(s) 1437, 1472
CviQI GTAC 4 cut(s) 53, 356, 1079, 1441
DdeI CTNAG 3 cut(s) 829, 862, 898
DpnI GATC 5 cut(s) 27, 456, 674, 692, 824
DpnII GATC 5 cut(s) 25, 454, 672, 690, 822
Eam1104I CTCTTC 1 cut(s) 1119
EarI CTCTTC 1 cut(s) 1119
Eco57I CTGAAG 4 cut(s) 140, 795, 1077, 1275
Eco88I CYCGRG 1 cut(s) 110
EcoRI GAATTC 1 cut(s) 780
EcoRII CCWGG 1 cut(s) 1257
EcoT22I ATGCAT 4 cut(s) 73, 509, 700, 1046
FalI AAGNNNNNCTT 2 cut(s) 629, 661
FauNDI CATATG 2 cut(s) 345, 1249
FbaI TGATCA 1 cut(s) 822
FblI GTMKAC 2 cut(s) 739, 936
FspBI CTAG 3 cut(s) 602, 1013, 1022
GlaI GCGC 2 cut(s) 298, 661
GsaI CCCAGC 1 cut(s) 1343
GsuI CTGGAG 2 cut(s) 943, 1341
HaeII RGCGCY 1 cut(s) 663
HaeIII GGCC 2 cut(s) 145, 429
HapII CCGG 2 cut(s) 47, 999
HhaI GCGC 2 cut(s) 299, 662
Hin6I GCGC 2 cut(s) 297, 660
HinP1I GCGC 2 cut(s) 297, 660
HincII GTYRAC 1 cut(s) 1255
HindII GTYRAC 1 cut(s) 1255
HindIII AAGCTT 1 cut(s) 1425
HinfI GANTC 7 cut(s) 32, 543, 756, 798, 943, 1344, 1383
HpaI GTTAAC 1 cut(s) 1255
HpaII CCGG 2 cut(s) 47, 999
HphI GGTGA 1 cut(s) 27
Hpy166II GTNNAC 9 cut(s) 356, 358, 607, 740, 937, 1138, 1229, 1255, 1441
Hpy188I TCNGA 3 cut(s) 722, 1244, 1432
Hpy188III TCNNGA 6 cut(s) 29, 452, 458, 496, 540, 670
Hpy8I GTNNAC 9 cut(s) 356, 358, 607, 740, 937, 1138, 1229, 1255, 1441
HpyAV CCTTC 5 cut(s) 407, 741, 819, 884, 1052
HpyCH4III ACNGT 3 cut(s) 339, 1099, 1112
HpyF10VI GCNNNNNNNGC 8 cut(s) 133, 142, 242, 473, 668, 1022, 1162, 1424
HpyF3I CTNAG 3 cut(s) 829, 862, 898
HspAI GCGC 2 cut(s) 297, 660
Ksp22I TGATCA 1 cut(s) 822
KspAI GTTAAC 1 cut(s) 1255
Kzo9I GATC 5 cut(s) 25, 454, 672, 690, 822
LmnI GCTCC 2 cut(s) 1244, 1262
Lsp1109I GCAGC 8 cut(s) 136, 139, 220, 242, 482, 649, 977, 1060
LweI GCATC 1 cut(s) 266
MaeI CTAG 3 cut(s) 602, 1013, 1022
MaeIII GTNAC 2 cut(s) 262, 580
MalI GATC 5 cut(s) 27, 456, 674, 692, 824
MboI GATC 5 cut(s) 25, 454, 672, 690, 822
MboII GAAGA 4 cut(s) 23, 147, 1136, 1432
MfeI CAATTG 1 cut(s) 989
MflI RGATCY 2 cut(s) 25, 454
MhlI GDGCHC 1 cut(s) 609
MluCI AATT 6 cut(s) 160, 440, 462, 780, 787, 989
MlyI GAGTC 1 cut(s) 792
MmeI TCCRAC 2 cut(s) 550, 1126
MnlI CCTC 9 cut(s) 360, 411, 650, 760, 907, 957, 1120, 1214, 1316
Mph1103I ATGCAT 4 cut(s) 73, 509, 700, 1046
MseI TTAA 2 cut(s) 918, 1254
MspA1I CMGCKG 2 cut(s) 233, 476
MspI CCGG 2 cut(s) 47, 999
MspR9I CCNGG 2 cut(s) 1000, 1259
MunI CAATTG 1 cut(s) 989
Mva1269I GAATGC 2 cut(s) 953, 1281
MvaI CCWGG 1 cut(s) 1259
MvnI CGCG 2 cut(s) 671, 1169
MwoI GCNNNNNNNGC 8 cut(s) 133, 142, 242, 473, 668, 1022, 1162, 1424
NciI CCSGG 1 cut(s) 1000
NdeI CATATG 2 cut(s) 345, 1249
NdeII GATC 5 cut(s) 25, 454, 672, 690, 822
NlaIV GGNNCC 2 cut(s) 570, 997
NmuCI GTSAC 1 cut(s) 262
NruI TCGCGA 1 cut(s) 671
NsiI ATGCAT 4 cut(s) 73, 509, 700, 1046
NspI RCATGY 4 cut(s) 71, 364, 396, 927
NspV TTCGAA 1 cut(s) 846
PaeI GCATGC 1 cut(s) 396
PciI ACATGT 1 cut(s) 923
PctI GAATGC 2 cut(s) 953, 1281
PfeI GAWTC 6 cut(s) 32, 543, 756, 943, 1344, 1383
PinAI ACCGGT 1 cut(s) 46
PleI GAGTC 1 cut(s) 792
PpsI GAGTC 1 cut(s) 792
PscI ACATGT 1 cut(s) 923
Psp6I CCWGG 1 cut(s) 1257
PspFI CCCAGC 1 cut(s) 1339
PspGI CCWGG 1 cut(s) 1257
PspN4I GGNNCC 2 cut(s) 570, 997
PspPI GGNCC 1 cut(s) 143
PsrI GAACNNNNNNTAC 2 cut(s) 57, 89
PstI CTGCAG 1 cut(s) 25
PstNI CAGNNNCTG 2 cut(s) 266, 476
PsuI RGATCY 2 cut(s) 25, 454
PvuII CAGCTG 1 cut(s) 233
RruI TCGCGA 1 cut(s) 671
RsaI GTAC 4 cut(s) 54, 357, 1080, 1442
RsaNI GTAC 4 cut(s) 53, 356, 1079, 1441
SaqAI TTAA 2 cut(s) 918, 1254
Sau3AI GATC 5 cut(s) 25, 454, 672, 690, 822
Sau96I GGNCC 1 cut(s) 143
SchI GAGTC 1 cut(s) 792
ScrFI CCNGG 2 cut(s) 1000, 1259
SduI GDGCHC 1 cut(s) 609
SfaNI GCATC 1 cut(s) 266
SfcI CTRYAG 1 cut(s) 21
SfuI TTCGAA 1 cut(s) 846
SpeI ACTAGT 1 cut(s) 601
SphI GCATGC 1 cut(s) 396
Sse9I AATT 6 cut(s) 160, 440, 462, 780, 787, 989
SsiI CCGC 4 cut(s) 146, 468, 474, 1169
SspMI CTAG 3 cut(s) 602, 1013, 1022
StyD4I CCNGG 2 cut(s) 998, 1257
TaaI ACNGT 3 cut(s) 339, 1099, 1112
TaqI TCGA 3 cut(s) 30, 846, 1093
TasI AATT 6 cut(s) 160, 440, 462, 780, 787, 989
TatI WGTACW 3 cut(s) 355, 1078, 1440
TauI GCSGC 3 cut(s) 148, 470, 476
TfiI GAWTC 6 cut(s) 32, 543, 756, 943, 1344, 1383
Tru1I TTAA 2 cut(s) 918, 1254
Tru9I TTAA 2 cut(s) 918, 1254
TscAI CASTG 3 cut(s) 271, 967, 1324
TseFI GTSAC 1 cut(s) 262
TseI GCWGC 8 cut(s) 124, 127, 230, 233, 470, 662, 965, 1073
Tsp45I GTSAC 1 cut(s) 262
TspDTI ATGAA 2 cut(s) 764, 781
TspGWI ACGGA 1 cut(s) 117
TspRI CASTG 3 cut(s) 271, 967, 1324
VneI GTGCAC 1 cut(s) 605
XapI RAATTY 3 cut(s) 160, 440, 780
XceI RCATGY 4 cut(s) 71, 364, 396, 927
XmiI GTMKAC 2 cut(s) 739, 936
XspI CTAG 3 cut(s) 602, 1013, 1022
Zsp2I ATGCAT 4 cut(s) 73, 509, 700, 1046
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.