Rh4BG111600

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0000521
Physical Location & Seq
Reverse (-)
18846744 .. 18849643
2900 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG111600.1

Sequence Viewer

Length: 1470 bp
ATGGATGCAGGGGAACAGAGAGGCGGTCTTGAATCGCCCTTGATTCCTGATCCACAAGAACATGCTCTACAATCAAGAAAGGGTTTGCTGAATAAAGATGAAATCGTCGAGGAAGTGAAGAAGCAGCTGTTGTTAGCAGGGCCACTTGTGTCATCAAATTTCCTGCTATTTGGTATGCAAGTTATTTCAGTCATGTATGTTGGTCATCTCGGGGAGCTATCACTTGCAGGTGCTTCAATGGCCACTTCATTCGCTTCAGTCACCGGTTTGAGCTTGATAATGGGAATGTGTAGCGCACTGGACACTTTCTGTGGTCAATCCTATGGAGCGAAACAGTATCGGATGCTTGGTATACATATGCAGAGGGCAATGCTTGTTCTTTTGCTGGTGAGCATTCCTCTTGCGATTATATGGGCCAATGCAGGAAGGATTCTTCAGTTCTTGGGTCAAGATCCAGATATATCTGCTGCTGCCGGAGATTATGCTCGTTTGATGATACCATGCATTTTCGCTTATGCAATCCTACAATGTCATGCTAGATTCCTGCAGACACAAAACAATGTGGTTCCGATGATTGTTAGCACCGGAACTGCAACACTGCTACACTTGCTTATCTGTTGGCTTCTGGTATACAAGACCAGCCTCGGATATAAAGGCGCTGCTGTGGCAAACGCCATCACCTATTGGATCAATGCATTGTTATTGTTTCTTTATGTCAGAATCTCTCCATCTTGCAAGTGCACATGGACTGGATTCTCAAAGGAGGCCTTCCATGGACTTCCCACTTTTTTAAGACTATCCATTCCTTCAGCTGTCATGCTTAGCTTAGAGATTTGGTCATTTGAAATGTTGGTTCTTGTATCTGGTTTTCTTCCAAATCCAAAGCTTGAAACCTCAGTCCTGTCAATCAGCCTTAACACATGTGCAATGGCATACATGATTCCCCTTGCATTCAGTGGTGCAGGGAGCACAAGAGTATCAAATCAGTTGGGTGCCGGGCAACCTAGACTAGCACGTCTAGCTGCATGTGTTTCACTATGCCTTGTTGTTATTGAAGGCATTGTTGTTGCTGCTGTCATGATATTGGGTCGAAAAGTATGGGGATACTGTTACAGCAATGAAAAACAAGTTGTGAATTATGTTAGTGAAATGTTGATTTTTGTTGCAATATCCCACTTTGTTGATGGACCTCAATCTGTTCTCTCAGGTGTCATAAGAGGAAGTGGGCAACAGAAGATTGGAGCATATGTTAATCTGGGAGCTTATTATCTTCTAGGCATACCTACTGGACTAGTATTTGCTTTTGGCCTCCACATTGGAGGAAAGGGACTTTGGATAGGAATTGTCGTCGCGCTATTCGTGCAAGCACTATGTCTTGCAATCATAGTCATATGCACAGATTGGGATAAAGAAGTGAAGAAAGCTTCTGATAGGGTGCACAATGCAGTCGGCGTGGGTGATGTGTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

489

Amino Acids

52.62

Weight (kDa)

8.49

Isoelectric Point (pI)

27.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 48 - 208 2.5e-38 MatE
MatE PF01554 269 - 430 1.8e-26 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000521)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g09990 FvH4_4g09990 FvH4_4g09990 FvH4_4g09990 FvH4_7g32860 FvH4_7g32860 FvH4_7g32860 FvH4_7g32860
malus_domestica MD11G1148000.v1.1 MD13G1266200.v1.1 MD14G1231800.v1.1
prunus_persica Prupe.1G090100_v2.0.a1 Prupe.1G090100_v2.0.a1 Prupe.1G090100_v2.0.a1 Prupe.1G090100_v2.0.a1 Prupe.1G090100_v2.0.a1 Prupe.1G090300_v2.0.a1 Prupe.1G090300_v2.0.a1 Prupe.1G090300_v2.0.a1 Prupe.1G090300_v2.0.a1 Prupe.1G090300_v2.0.a1
pyrus_communis pycom13g23330
rosa_chinensis RchiOBHm_Chr4g0403401 RchiOBHm_Chr4g0403411 RchiOBHm_Chr4g0403421 RchiOBHm_Chr4g0403461 RchiOBHm_Chr4g0403631 RchiOBHm_Chr7g0223601 RchiOBHm_Chr7g0238161
rosa_laevigata RLG00000000937 RLG00000001957 RLG00000008965 RLG00000008975 RLG00000008976 RLG00000008978
rosa_multiflora Rmu_co8335743.1_g000001 Rmu_sc0005734.1_g000001 Rmu_sc0007727.1_g000011 Rmu_sc0011775.1_g000002 Rmu_sc0011775.1_g000005 Rmu_sc0028062.1_g000002
rosa_roxburghii Rroxscaffold_3G00223300 Rroxscaffold_5G00348050 Rroxscaffold_5G00348060 Rroxscaffold_5G00348120 Rroxscaffold_5G00348240
rosa_rugosa Rorug04G0042300 Rorug04G0042400 Rorug04G0042500 Rorug04G0042600.1 Rorug04G0042700 Rorug04G0042800 Rorug04G0042900 Rorug04G0043000 Rorug04G0043100 Rorug04G0043200 Rorug04G0043300 Rorug04G0043400 Rorug04G0044700 Rorug07G0310600 Rorug07G0310700 Rorug07G0310700
rosa_samantha Rh4AG118700 Rh4AG118800 Rh4AG118900 Rh4AG119000 Rh4BG111600 Rh4BG111800 Rh4BG111900 Rh4BG112900 Rh4CG126600 Rh4CG126900 Rh4CG127800 Rh4DG111300 Rh4DG111400 Rh4DG111500 Rh7AG466500 Rh7BG352200 Rh7BG436900 Rh7CG484200 Rh7DG452600
rosa_wichuraiana Rw4G009630 Rw4G009640 Rw4G009650 Rw4G009710 Rw7G038620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 218
AasI GACNNNNNNGTC 1 cut(s) 1014
Acc36I ACCTGC 1 cut(s) 218
AccB1I GGYRCC 1 cut(s) 992
AccI GTMKAC 2 cut(s) 352, 630
AccII CGCG 1 cut(s) 1352
AciI CCGC 1 cut(s) 24
AclWI GGATC 3 cut(s) 44, 446, 695
AcoI YGGCCR 1 cut(s) 240
AcsI RAATTY 1 cut(s) 157
AcuI CTGAAG 3 cut(s) 240, 419, 792
AflIII ACRYGT 1 cut(s) 920
AgeI ACCGGT 1 cut(s) 263
AgsI TTSAA 5 cut(s) 32, 237, 845, 890, 1055
AhlI ACTAGT 1 cut(s) 1291
AjiI CACGTC 1 cut(s) 1016
AjuI GAANNNNNNNTTGG 2 cut(s) 1315, 1347
AluBI AGCT 9 cut(s) 127, 217, 273, 812, 825, 886, 1022, 1262, 1424
AluI AGCT 9 cut(s) 127, 217, 273, 812, 825, 886, 1022, 1262, 1424
Alw21I GWGCWC 3 cut(s) 743, 971, 1440
Alw44I GTGCAC 2 cut(s) 739, 1436
AlwI GGATC 3 cut(s) 44, 446, 695
Ama87I CYCGRG 1 cut(s) 209
AoxI GGCC 5 cut(s) 140, 240, 414, 765, 1306
ApaLI GTGCAC 2 cut(s) 739, 1436
ApeKI GCWGC 6 cut(s) 124, 467, 470, 659, 1022, 1070
ApoI RAATTY 1 cut(s) 157
AsiGI ACCGGT 1 cut(s) 263
AspLEI GCGC 3 cut(s) 296, 659, 1354
AspS9I GGNCC 3 cut(s) 140, 414, 1187
AsuC2I CCSGG 1 cut(s) 997
AsuHPI GGTGA 4 cut(s) 253, 400, 670, 1469
AvaI CYCGRG 1 cut(s) 209
AvaII GGWCC 1 cut(s) 1187
BaeGI GKGCMC 2 cut(s) 743, 1440
BaeI ACNNNNGTAYC 2 cut(s) 960, 993
BalI TGGCCA 1 cut(s) 242
BanI GGYRCC 1 cut(s) 992
Bbv12I GWGCWC 3 cut(s) 743, 971, 1440
BbvI GCAGC 6 cut(s) 136, 454, 457, 646, 1009, 1057
BccI CCATC 3 cut(s) 683, 736, 1178
BciVI GTATCC 1 cut(s) 1097
BcnI CCSGG 1 cut(s) 997
BcuI ACTAGT 1 cut(s) 1291
BfaI CTAG 6 cut(s) 537, 1005, 1010, 1019, 1274, 1292
BfmI CTRYAG 1 cut(s) 545
BfoI RGCGCY 1 cut(s) 660
BfuAI ACCTGC 1 cut(s) 218
BfuI GTATCC 1 cut(s) 1097
BisI GCNGC 6 cut(s) 125, 468, 471, 660, 1023, 1071
BlpI GCTNAGC 1 cut(s) 821
BlsI GCNGC 6 cut(s) 126, 469, 472, 661, 1024, 1072
Bme1390I CCNGG 1 cut(s) 997
Bme18I GGWCC 1 cut(s) 1187
BmeT110I CYCGRG 1 cut(s) 209
BmgBI CACGTC 1 cut(s) 1016
BmgT120I GGNCC 3 cut(s) 140, 414, 1187
BmiI GGNNCC 2 cut(s) 567, 994
BmrFI CCNGG 1 cut(s) 997
BmsI GCATC 1 cut(s) 333
BplI GAGNNNNNCTC 2 cut(s) 382, 414
Bpu1102I GCTNAGC 1 cut(s) 821
BpuMI CCSGG 1 cut(s) 997
BsaJI CCNNGG 2 cut(s) 643, 772
BsaWI WCCGGW 2 cut(s) 263, 584
BsaXI ACNNNNNCTCC 2 cut(s) 1233, 1263
Bse118I RCCGGY 1 cut(s) 263
Bse1I ACTGG 3 cut(s) 303, 754, 1291
Bse3DI GCAATG 3 cut(s) 375, 933, 1123
BseDI CCNNGG 2 cut(s) 643, 772
BseGI GGATG 2 cut(s) 10, 348
BseMI GCAATG 3 cut(s) 375, 933, 1123
BseMII CTCAG 2 cut(s) 909, 1218
BseNI ACTGG 3 cut(s) 303, 754, 1291
BseSI GKGCMC 2 cut(s) 743, 1440
BseXI GCAGC 6 cut(s) 136, 454, 457, 646, 1009, 1057
BsgI GTGCAG 1 cut(s) 981
Bsh1236I CGCG 1 cut(s) 1352
BshFI GGCC 5 cut(s) 142, 242, 416, 767, 1308
BshNI GGYRCC 1 cut(s) 992
BshTI ACCGGT 1 cut(s) 263
BsiHKAI GWGCWC 3 cut(s) 743, 971, 1440
BsiHKCI CYCGRG 1 cut(s) 209
BsiSI CCGG 4 cut(s) 264, 474, 585, 996
BslFI GGGAC 1 cut(s) 1341
BsmFI GGGAC 1 cut(s) 1341
BsmI GAATGC 2 cut(s) 393, 950
BsnI GGCC 5 cut(s) 142, 242, 416, 767, 1308
BsoBI CYCGRG 1 cut(s) 209
Bsp1286I GDGCHC 3 cut(s) 743, 971, 1440
Bsp143I GATC 3 cut(s) 49, 451, 687
Bsp1720I GCTNAGC 1 cut(s) 821
Bsp19I CCATGG 1 cut(s) 772
BspACI CCGC 1 cut(s) 24
BspANI GGCC 5 cut(s) 142, 242, 416, 767, 1308
BspCNI CTCAG 2 cut(s) 908, 1217
BspFNI CGCG 1 cut(s) 1352
BspHI TCATGA 1 cut(s) 1077
BspLI GGNNCC 2 cut(s) 567, 994
BspMAI CTGCAG 1 cut(s) 549
BspMI ACCTGC 1 cut(s) 218
BspPI GGATC 3 cut(s) 44, 446, 695
BspT107I GGYRCC 1 cut(s) 992
BsrDI GCAATG 3 cut(s) 375, 933, 1123
BsrFI RCCGGY 1 cut(s) 263
BsrI ACTGG 3 cut(s) 303, 754, 1291
BssAI RCCGGY 1 cut(s) 263
BssECI CCNNGG 2 cut(s) 643, 772
BssMI GATC 3 cut(s) 49, 451, 687
BssNAI GTATAC 2 cut(s) 353, 631
BssT1I CCWWGG 1 cut(s) 772
Bst1107I GTATAC 2 cut(s) 353, 631
Bst4CI ACNGT 2 cut(s) 336, 1109
BstC8I GCNNGC 1 cut(s) 1365
BstDEI CTNAG 4 cut(s) 821, 826, 895, 1204
BstDSI CCRYGG 1 cut(s) 772
BstF5I GGATG 2 cut(s) 10, 348
BstFNI CGCG 1 cut(s) 1352
BstH2I RGCGCY 1 cut(s) 660
BstHHI GCGC 3 cut(s) 296, 659, 1354
BstKTI GATC 3 cut(s) 52, 454, 690
BstMBI GATC 3 cut(s) 49, 451, 687
BstMWI GCNNNNNNNGC 5 cut(s) 239, 607, 665, 1019, 1360
BstNSI RCATGY 3 cut(s) 65, 924, 1029
BstSCI CCNGG 1 cut(s) 995
BstSFI CTRYAG 1 cut(s) 545
BstSLI GKGCMC 2 cut(s) 743, 1440
BstUI CGCG 1 cut(s) 1352
BstV1I GCAGC 6 cut(s) 136, 454, 457, 646, 1009, 1057
BstX2I RGATCY 1 cut(s) 451
BstYI RGATCY 1 cut(s) 451
BstZ17I GTATAC 2 cut(s) 353, 631
BsuI GTATCC 1 cut(s) 1097
BsuRI GGCC 5 cut(s) 142, 242, 416, 767, 1308
BtgI CCRYGG 1 cut(s) 772
BtrI CACGTC 1 cut(s) 1016
BtsCI GGATG 2 cut(s) 10, 348
BtsI GCAGTG 1 cut(s) 596
BtsIMutI CAGTG 3 cut(s) 296, 596, 961
BveI ACCTGC 1 cut(s) 218
Cac8I GCNNGC 1 cut(s) 1365
CciI TCATGA 1 cut(s) 1077
CfoI GCGC 3 cut(s) 296, 659, 1354
Cfr10I RCCGGY 1 cut(s) 263
Cfr13I GGNCC 3 cut(s) 140, 414, 1187
CspAI ACCGGT 1 cut(s) 263
DdeI CTNAG 4 cut(s) 821, 826, 895, 1204
DpnI GATC 3 cut(s) 51, 453, 689
DpnII GATC 3 cut(s) 49, 451, 687
DrdI GACNNNNNNGTC 1 cut(s) 1014
DseDI GACNNNNNNGTC 1 cut(s) 1014
EaeI YGGCCR 1 cut(s) 240
Eco130I CCWWGG 1 cut(s) 772
Eco147I AGGCCT 1 cut(s) 767
Eco47I GGWCC 1 cut(s) 1187
Eco57I CTGAAG 3 cut(s) 240, 419, 792
Eco88I CYCGRG 1 cut(s) 209
EcoT14I CCWWGG 1 cut(s) 772
EcoT22I ATGCAT 2 cut(s) 506, 697
ErhI CCWWGG 1 cut(s) 772
FalI AAGNNNNNCTT 2 cut(s) 752, 784
FaqI GGGAC 1 cut(s) 1341
FauNDI CATATG 3 cut(s) 357, 1246, 1391
FblI GTMKAC 2 cut(s) 352, 630
Fnu4HI GCNGC 6 cut(s) 125, 468, 471, 660, 1023, 1071
FokI GGATG 2 cut(s) 17, 355
Fsp4HI GCNGC 6 cut(s) 125, 468, 471, 660, 1023, 1071
FspBI CTAG 6 cut(s) 537, 1005, 1010, 1019, 1274, 1292
GlaI GCGC 3 cut(s) 295, 658, 1353
GluI GCNGC 6 cut(s) 125, 468, 471, 660, 1023, 1071
HaeII RGCGCY 1 cut(s) 660
HaeIII GGCC 5 cut(s) 142, 242, 416, 767, 1308
HapII CCGG 4 cut(s) 264, 474, 585, 996
HhaI GCGC 3 cut(s) 296, 659, 1354
Hin6I GCGC 3 cut(s) 294, 657, 1352
HinP1I GCGC 3 cut(s) 294, 657, 1352
HindIII AAGCTT 2 cut(s) 884, 1422
HinfI GANTC 7 cut(s) 32, 43, 430, 540, 720, 753, 940
HpaII CCGG 4 cut(s) 264, 474, 585, 996
HphI GGTGA 4 cut(s) 253, 400, 670, 1469
Hpy166II GTNNAC 4 cut(s) 353, 631, 741, 1438
Hpy188I TCNGA 5 cut(s) 342, 570, 647, 719, 1429
Hpy188III TCNNGA 6 cut(s) 29, 47, 75, 449, 455, 1078
Hpy8I GTNNAC 4 cut(s) 353, 631, 741, 1438
Hpy99I CGWCG 2 cut(s) 110, 1352
HpyAV CCTTC 4 cut(s) 420, 778, 816, 1049
HpyCH4III ACNGT 2 cut(s) 336, 1109
HpyCH4IV ACGT 1 cut(s) 1015
HpyF10VI GCNNNNNNNGC 5 cut(s) 239, 607, 665, 1019, 1360
HpyF3I CTNAG 4 cut(s) 821, 826, 895, 1204
HpySE526I ACGT 1 cut(s) 1015
HspAI GCGC 3 cut(s) 294, 657, 1352
Kzo9I GATC 3 cut(s) 49, 451, 687
LmnI GCTCC 5 cut(s) 214, 326, 966, 1241, 1259
Lsp1109I GCAGC 6 cut(s) 136, 454, 457, 646, 1009, 1057
LweI GCATC 1 cut(s) 333
MaeI CTAG 6 cut(s) 537, 1005, 1010, 1019, 1274, 1292
MaeII ACGT 1 cut(s) 1015
MaeIII GTNAC 2 cut(s) 259, 1109
MalI GATC 3 cut(s) 51, 453, 689
MboI GATC 3 cut(s) 49, 451, 687
MboII GAAGA 6 cut(s) 130, 425, 863, 1246, 1262, 1429
MflI RGATCY 1 cut(s) 451
MhlI GDGCHC 3 cut(s) 743, 971, 1440
MlsI TGGCCA 1 cut(s) 242
MluCI AATT 3 cut(s) 157, 1135, 1341
MluNI TGGCCA 1 cut(s) 242
Mox20I TGGCCA 1 cut(s) 242
Mph1103I ATGCAT 2 cut(s) 506, 697
MscI TGGCCA 1 cut(s) 242
MseI TTAA 3 cut(s) 791, 915, 1251
Msp20I TGGCCA 1 cut(s) 242
MspA1I CMGCKG 2 cut(s) 127, 812
MspI CCGG 4 cut(s) 264, 474, 585, 996
MspR9I CCNGG 1 cut(s) 997
Mva1269I GAATGC 2 cut(s) 393, 950
MvnI CGCG 1 cut(s) 1352
MwoI GCNNNNNNNGC 5 cut(s) 239, 607, 665, 1019, 1360
NciI CCSGG 1 cut(s) 997
NcoI CCATGG 1 cut(s) 772
NdeI CATATG 3 cut(s) 357, 1246, 1391
NdeII GATC 3 cut(s) 49, 451, 687
NlaIV GGNNCC 2 cut(s) 567, 994
NmuCI GTSAC 1 cut(s) 259
NsiI ATGCAT 2 cut(s) 506, 697
NspI RCATGY 3 cut(s) 65, 924, 1029
PagI TCATGA 1 cut(s) 1077
PaqCI CACCTGC 1 cut(s) 218
PceI AGGCCT 1 cut(s) 767
PciI ACATGT 1 cut(s) 920
PcsI WCGNNNNNNNCGW 1 cut(s) 1356
PctI GAATGC 2 cut(s) 393, 950
PfeI GAWTC 7 cut(s) 32, 43, 430, 540, 720, 753, 940
PinAI ACCGGT 1 cut(s) 263
PkrI GCNGC 6 cut(s) 126, 469, 472, 661, 1024, 1072
PscI ACATGT 1 cut(s) 920
PspN4I GGNNCC 2 cut(s) 567, 994
PspPI GGNCC 3 cut(s) 140, 414, 1187
PsrI GAACNNNNNNTAC 2 cut(s) 51, 83
PstI CTGCAG 1 cut(s) 549
PsuI RGATCY 1 cut(s) 451
PvuII CAGCTG 2 cut(s) 127, 812
SaqAI TTAA 3 cut(s) 791, 915, 1251
SatI GCNGC 6 cut(s) 125, 468, 471, 660, 1023, 1071
Sau3AI GATC 3 cut(s) 49, 451, 687
Sau96I GGNCC 3 cut(s) 140, 414, 1187
ScrFI CCNGG 1 cut(s) 997
SduI GDGCHC 3 cut(s) 743, 971, 1440
SfaNI GCATC 1 cut(s) 333
SfcI CTRYAG 1 cut(s) 545
SinI GGWCC 1 cut(s) 1187
SpeI ACTAGT 1 cut(s) 1291
Sse9I AATT 3 cut(s) 157, 1135, 1341
SseBI AGGCCT 1 cut(s) 767
SsiI CCGC 1 cut(s) 24
SspMI CTAG 6 cut(s) 537, 1005, 1010, 1019, 1274, 1292
StuI AGGCCT 1 cut(s) 767
StyD4I CCNGG 1 cut(s) 995
StyI CCWWGG 1 cut(s) 772
TaaI ACNGT 2 cut(s) 336, 1109
TaiI ACGT 1 cut(s) 1018
TaqI TCGA 2 cut(s) 108, 1090
TasI AATT 3 cut(s) 157, 1135, 1341
TfiI GAWTC 7 cut(s) 32, 43, 430, 540, 720, 753, 940
Tru1I TTAA 3 cut(s) 791, 915, 1251
Tru9I TTAA 3 cut(s) 791, 915, 1251
TscAI CASTG 3 cut(s) 303, 603, 961
TseFI GTSAC 1 cut(s) 259
TseI GCWGC 6 cut(s) 124, 467, 470, 659, 1022, 1070
Tsp45I GTSAC 1 cut(s) 259
TspDTI ATGAA 3 cut(s) 114, 237, 1134
TspRI CASTG 3 cut(s) 303, 603, 961
VneI GTGCAC 2 cut(s) 739, 1436
VpaK11BI GGWCC 1 cut(s) 1187
XapI RAATTY 1 cut(s) 157
XceI RCATGY 3 cut(s) 65, 924, 1029
XcmI CCANNNNNNNNNTGG 1 cut(s) 1181
XmiI GTMKAC 2 cut(s) 352, 630
XspI CTAG 6 cut(s) 537, 1005, 1010, 1019, 1274, 1292
Zsp2I ATGCAT 2 cut(s) 506, 697
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.