Prupe.1G090300_v2.0.a1

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0000521
Physical Location & Seq
Reverse (-)
6806288 .. 6810045
3758 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G090300.7

Sequence Viewer

Length: 1470 bp
ATGGATGCCGAAGAACAAGCAGCTGGTCTTGAATCTCCCTTGCTTCCAGCTCCTCAAGAACATGAATCACAAACAACAAAGGGAAAACAAACCAAAGATGAGATTATCAAGGAGGTGAAGAAGCAGCTGTCTTTAGCAGGGCCACTTGTGTCATCAAATTTCTTGCTCTTCGGTATGCAGGTGATTTCAGTTATGTATGTTGGCCATCTTGGAGAGCTGGCACTTGCAGGGGCTTCCATGGCCACTTCATTTGCTACAGTCACTGGTTTGAGCTTGATTATAGGAATGAGCTTTGCATTAGACACCTTCTGTGGCCAGTCCTATGGAGCAAAACAGTATCATATGCTTGGCATACACTTACAGAGAGCAATGCTTGTTCTTCTACTGGTCTGCATTCCTCTTTCAATTATATGGTTCAATGCTGGTCATATTCTTGAGTTCTTGGGTCAAGATCCAGAAATAGCAGCTGCTGCTGGAAGTTACGCTCGTTTCCTGATACCATGCCTCTTCGCTTATGCAGTCAACCAATGCCACTCCAAATTTTTGCAAAGTCAAAACAACGTGGTTCCCATGATTGTTAGCACAGGCATTGCAACACTGTTGCACTTGATTGTCTGTGGGGTTTTGGTATACAAGACTAGCCTTGGATATAGAGGTGCTGCGGTGGCAAACTCCATCTCGTATTGGATCAACGCGTTGACATTGGTTATTTATGTCAGAGTTTCTCCCTCTTGCAAGCACACATGGACCGGGTTCTCAAAGGATGCCTTCCATGGAATTGTCAATTTTCTCAAACTCTCTGTTCCTTCAGCTGTAATGATCAGCTTAGAAATCTGGTCATTCGAAATGATGGTCCTCTTATCTGGTTTTCTTCCCAATCCAAAGCTTGAAACCTCAGTCCTGTCTATCAGCCTTAACACATGCTCAATGGTTTTCATGATTCCCATGGCATTCAGTGGTGCAGCAAGCACAAGGGTCTCCAATCAATTGGGTGCTGGGCAACCGCGACTCGCAGTTCTATCTGTACGTGTTGCACTCTCCATTGTGATTCTTGAGGGCATTTTGATTGGCACTGTCCTCATTTTGGGTCGAAAAGTTTGGGGCTATTGTTACAGCAAAGAAATAGAAGTTGTGAATTATGTTGGAGAAATGTTGATTTTGGTTGCAATATCCCACTTTTTTGATGGACTTCAGTCTGTGCTTTCAGGTGTCATAAGAGGAAGTGGACAACAAAAGATTGGGGCATATGTTAATCTGGGAGCTTACTATCTTATGGGCATCCCAACTGCAGTTGTATTAGCTTTTGTCCTGCACCTTGGAGGGAAGGGTCTTTGGACCGGAATTACTGTCGCTCTATTTTCCCAGACACTATTTCTTGCAATCATAATCACACGCACAGATTGGGAGAAAGAAGTGAAGAAAGCATCTGATAGAGTGTACGACACAAAGACCGCGGCCAATGTGTCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

490

Amino Acids

52.8

Weight (kDa)

8.5

Isoelectric Point (pI)

25.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000521)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g09990 FvH4_4g09990 FvH4_4g09990 FvH4_4g09990 FvH4_7g32860 FvH4_7g32860 FvH4_7g32860 FvH4_7g32860
malus_domestica MD11G1148000.v1.1 MD13G1266200.v1.1 MD14G1231800.v1.1
prunus_persica Prupe.1G090100_v2.0.a1 Prupe.1G090100_v2.0.a1 Prupe.1G090100_v2.0.a1 Prupe.1G090100_v2.0.a1 Prupe.1G090100_v2.0.a1 Prupe.1G090300_v2.0.a1 Prupe.1G090300_v2.0.a1 Prupe.1G090300_v2.0.a1 Prupe.1G090300_v2.0.a1 Prupe.1G090300_v2.0.a1
pyrus_communis pycom13g23330
rosa_chinensis RchiOBHm_Chr4g0403401 RchiOBHm_Chr4g0403411 RchiOBHm_Chr4g0403421 RchiOBHm_Chr4g0403461 RchiOBHm_Chr4g0403631 RchiOBHm_Chr7g0223601 RchiOBHm_Chr7g0238161
rosa_laevigata RLG00000000937 RLG00000001957 RLG00000008965 RLG00000008975 RLG00000008976 RLG00000008978
rosa_multiflora Rmu_co8335743.1_g000001 Rmu_sc0005734.1_g000001 Rmu_sc0007727.1_g000011 Rmu_sc0011775.1_g000002 Rmu_sc0011775.1_g000005 Rmu_sc0028062.1_g000002
rosa_roxburghii Rroxscaffold_3G00223300 Rroxscaffold_5G00348050 Rroxscaffold_5G00348060 Rroxscaffold_5G00348120 Rroxscaffold_5G00348240
rosa_rugosa Rorug04G0042300 Rorug04G0042400 Rorug04G0042500 Rorug04G0042600.1 Rorug04G0042700 Rorug04G0042800 Rorug04G0042900 Rorug04G0043000 Rorug04G0043100 Rorug04G0043200 Rorug04G0043300 Rorug04G0043400 Rorug04G0044700 Rorug07G0310600 Rorug07G0310700 Rorug07G0310700
rosa_samantha Rh4AG118700 Rh4AG118800 Rh4AG118900 Rh4AG119000 Rh4BG111600 Rh4BG111800 Rh4BG111900 Rh4BG112900 Rh4CG126600 Rh4CG126900 Rh4CG127800 Rh4DG111300 Rh4DG111400 Rh4DG111500 Rh7AG466500 Rh7BG352200 Rh7BG436900 Rh7CG484200 Rh7DG452600
rosa_wichuraiana Rw4G009630 Rw4G009640 Rw4G009650 Rw4G009710 Rw7G038620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 169
Acc36I ACCTGC 1 cut(s) 169
AccI GTMKAC 1 cut(s) 630
AccII CGCG 3 cut(s) 695, 1006, 1454
AciI CCGC 4 cut(s) 662, 1004, 1452, 1454
AclWI GGATC 2 cut(s) 446, 695
AcoI YGGCCR 4 cut(s) 202, 240, 313, 1455
AcsI RAATTY 2 cut(s) 157, 539
AcuI CTGAAG 2 cut(s) 792, 1175
AfaI GTAC 2 cut(s) 1026, 1439
AfiI CCNNNNNNNGG 1 cut(s) 1084
AflIII ACRYGT 2 cut(s) 693, 1027
AgsI TTSAA 4 cut(s) 32, 405, 418, 890
Alw26I GTCTC 1 cut(s) 982
AlwI GGATC 2 cut(s) 446, 695
AlwNI CAGNNNCTG 2 cut(s) 263, 470
AoxI GGCC 5 cut(s) 140, 202, 240, 313, 1455
ApeKI GCWGC 7 cut(s) 20, 124, 464, 467, 470, 659, 962
ApoI RAATTY 2 cut(s) 157, 539
ArsI GACNNNNNNTTYG 2 cut(s) 837, 869
AspS9I GGNCC 4 cut(s) 140, 747, 853, 1335
AsuC2I CCSGG 1 cut(s) 751
AsuHPI GGTGA 2 cut(s) 127, 193
AsuII TTCGAA 1 cut(s) 843
AvaII GGWCC 3 cut(s) 747, 853, 1335
BalI TGGCCA 3 cut(s) 204, 242, 315
BbvI GCAGC 7 cut(s) 32, 136, 454, 457, 476, 646, 974
BccI CCATC 4 cut(s) 213, 683, 844, 1178
BclI TGATCA 1 cut(s) 819
BcnI CCSGG 1 cut(s) 751
BcoDI GTCTC 1 cut(s) 982
BfaI CTAG 1 cut(s) 639
BfmI CTRYAG 2 cut(s) 255, 1287
BfuAI ACCTGC 1 cut(s) 169
BisI GCNGC 8 cut(s) 21, 125, 465, 468, 471, 660, 963, 1455
BlsI GCNGC 8 cut(s) 22, 126, 466, 469, 472, 661, 964, 1456
Bme1390I CCNGG 1 cut(s) 751
Bme18I GGWCC 3 cut(s) 747, 853, 1335
BmgT120I GGNCC 4 cut(s) 140, 747, 853, 1335
BmiI GGNNCC 1 cut(s) 567
BmrFI CCNGG 1 cut(s) 751
BmsI GCATC 3 cut(s) 754, 1287, 1433
BoxI GACNNNNGTC 1 cut(s) 1192
Bpu14I TTCGAA 1 cut(s) 843
BpuEI CTTGAG 3 cut(s) 39, 455, 1073
BpuMI CCSGG 1 cut(s) 751
BsaAI YACGTR 1 cut(s) 1028
BsaI GGTCTC 1 cut(s) 982
BsaJI CCNNGG 6 cut(s) 237, 643, 772, 945, 1315, 1452
BsaWI WCCGGW 1 cut(s) 1337
Bsc4I CCNNNNNNNGG 1 cut(s) 1084
Bse1I ACTGG 3 cut(s) 268, 316, 390
Bse3DI GCAATG 2 cut(s) 375, 588
BseDI CCNNGG 6 cut(s) 237, 643, 772, 945, 1315, 1452
BseGI GGATG 3 cut(s) 10, 769, 1278
BseLI CCNNNNNNNGG 1 cut(s) 1084
BseMI GCAATG 2 cut(s) 375, 588
BseMII CTCAG 1 cut(s) 909
BseNI ACTGG 3 cut(s) 268, 316, 390
BseRI GAGGAG 1 cut(s) 42
BseXI GCAGC 7 cut(s) 32, 136, 454, 457, 476, 646, 974
BseYI CCCAGC 1 cut(s) 995
BsgI GTGCAG 2 cut(s) 981, 1295
Bsh1236I CGCG 3 cut(s) 695, 1006, 1454
BshFI GGCC 5 cut(s) 142, 204, 242, 315, 1457
BsiSI CCGG 2 cut(s) 750, 1338
BslI CCNNNNNNNGG 1 cut(s) 1084
BsmAI GTCTC 1 cut(s) 982
BsmI GAATGC 2 cut(s) 393, 950
BsnI GGCC 5 cut(s) 142, 204, 242, 315, 1457
Bso31I GGTCTC 1 cut(s) 982
Bsp119I TTCGAA 1 cut(s) 843
Bsp143I GATC 3 cut(s) 451, 687, 819
Bsp19I CCATGG 3 cut(s) 237, 772, 945
BspACI CCGC 4 cut(s) 662, 1004, 1452, 1454
BspANI GGCC 5 cut(s) 142, 204, 242, 315, 1457
BspCNI CTCAG 1 cut(s) 908
BspFNI CGCG 3 cut(s) 695, 1006, 1454
BspHI TCATGA 1 cut(s) 936
BspLI GGNNCC 1 cut(s) 567
BspMAI CTGCAG 1 cut(s) 1291
BspMI ACCTGC 1 cut(s) 169
BspPI GGATC 2 cut(s) 446, 695
BspQI GCTCTTC 1 cut(s) 173
BspT104I TTCGAA 1 cut(s) 843
BspTNI GGTCTC 1 cut(s) 982
BsrDI GCAATG 2 cut(s) 375, 588
BsrI ACTGG 3 cut(s) 268, 316, 390
BssECI CCNNGG 6 cut(s) 237, 643, 772, 945, 1315, 1452
BssMI GATC 3 cut(s) 451, 687, 819
BssNAI GTATAC 1 cut(s) 631
BssT1I CCWWGG 5 cut(s) 237, 643, 772, 945, 1315
Bst1107I GTATAC 1 cut(s) 631
Bst4CI ACNGT 5 cut(s) 259, 336, 600, 1075, 1348
Bst6I CTCTTC 2 cut(s) 173, 512
BstAPI GCANNNNNTGC 1 cut(s) 470
BstBAI YACGTR 1 cut(s) 1028
BstBI TTCGAA 1 cut(s) 843
BstC8I GCNNGC 3 cut(s) 219, 737, 967
BstDEI CTNAG 2 cut(s) 826, 895
BstDSI CCRYGG 4 cut(s) 237, 772, 945, 1452
BstF5I GGATG 3 cut(s) 10, 769, 1278
BstFNI CGCG 3 cut(s) 695, 1006, 1454
BstKTI GATC 3 cut(s) 454, 690, 822
BstMAI GTCTC 1 cut(s) 982
BstMBI GATC 3 cut(s) 451, 687, 819
BstMWI GCNNNNNNNGC 3 cut(s) 239, 470, 665
BstNSI RCATGY 1 cut(s) 924
BstPAI GACNNNNGTC 1 cut(s) 1192
BstSCI CCNGG 1 cut(s) 749
BstSFI CTRYAG 2 cut(s) 255, 1287
BstUI CGCG 3 cut(s) 695, 1006, 1454
BstV1I GCAGC 7 cut(s) 32, 136, 454, 457, 476, 646, 974
BstX2I RGATCY 1 cut(s) 451
BstXI CCANNNNNNTGG 2 cut(s) 323, 988
BstYI RGATCY 1 cut(s) 451
BstZ17I GTATAC 1 cut(s) 631
BsuRI GGCC 5 cut(s) 142, 204, 242, 315, 1457
BtgI CCRYGG 4 cut(s) 237, 772, 945, 1452
BtsCI GGATG 3 cut(s) 10, 769, 1278
BtsIMutI CAGTG 4 cut(s) 261, 596, 961, 1071
BveI ACCTGC 1 cut(s) 169
Cac8I GCNNGC 3 cut(s) 219, 737, 967
CaiI CAGNNNCTG 2 cut(s) 263, 470
CciI TCATGA 1 cut(s) 936
Cfr13I GGNCC 4 cut(s) 140, 747, 853, 1335
Cfr42I CCGCGG 1 cut(s) 1455
Csp6I GTAC 2 cut(s) 1025, 1438
CspCI CAANNNNNGTGG 2 cut(s) 232, 267
CviAII CATG 9 cut(s) 62, 238, 501, 571, 744, 773, 921, 937, 946
CviQI GTAC 2 cut(s) 1025, 1438
DdeI CTNAG 2 cut(s) 826, 895
DpnI GATC 3 cut(s) 453, 689, 821
DpnII GATC 3 cut(s) 451, 687, 819
EaeI YGGCCR 4 cut(s) 202, 240, 313, 1455
Eam1104I CTCTTC 2 cut(s) 173, 512
EarI CTCTTC 2 cut(s) 173, 512
Eco130I CCWWGG 5 cut(s) 237, 643, 772, 945, 1315
Eco31I GGTCTC 1 cut(s) 982
Eco47I GGWCC 3 cut(s) 747, 853, 1335
Eco57I CTGAAG 2 cut(s) 792, 1175
EcoT14I CCWWGG 5 cut(s) 237, 643, 772, 945, 1315
ErhI CCWWGG 5 cut(s) 237, 643, 772, 945, 1315
FaeI CATG 9 cut(s) 65, 241, 504, 574, 747, 776, 924, 940, 949
FalI AAGNNNNNCTT 2 cut(s) 752, 784
FatI CATG 9 cut(s) 61, 237, 500, 570, 743, 772, 920, 936, 945
FauNDI CATATG 2 cut(s) 342, 1246
FbaI TGATCA 1 cut(s) 819
FblI GTMKAC 1 cut(s) 630
Fnu4HI GCNGC 8 cut(s) 21, 125, 465, 468, 471, 660, 963, 1455
FokI GGATG 3 cut(s) 17, 776, 1265
Fsp4HI GCNGC 8 cut(s) 21, 125, 465, 468, 471, 660, 963, 1455
FspBI CTAG 1 cut(s) 639
GluI GCNGC 8 cut(s) 21, 125, 465, 468, 471, 660, 963, 1455
GsaI CCCAGC 1 cut(s) 999
HaeIII GGCC 5 cut(s) 142, 204, 242, 315, 1457
HapII CCGG 2 cut(s) 750, 1338
Hin1II CATG 9 cut(s) 65, 241, 504, 574, 747, 776, 924, 940, 949
HincII GTYRAC 2 cut(s) 523, 699
HindII GTYRAC 2 cut(s) 523, 699
HindIII AAGCTT 1 cut(s) 884
HinfI GANTC 5 cut(s) 32, 65, 940, 1008, 1048
HpaII CCGG 2 cut(s) 750, 1338
HphI GGTGA 2 cut(s) 127, 193
Hpy166II GTNNAC 5 cut(s) 523, 631, 699, 1226, 1438
Hpy188I TCNGA 2 cut(s) 719, 1429
Hpy188III TCNNGA 8 cut(s) 29, 56, 434, 449, 455, 493, 937, 1052
Hpy8I GTNNAC 5 cut(s) 523, 631, 699, 1226, 1438
HpyAV CCTTC 4 cut(s) 316, 778, 816, 1318
HpyCH4III ACNGT 5 cut(s) 259, 336, 600, 1075, 1348
HpyCH4IV ACGT 2 cut(s) 561, 1027
HpyF10VI GCNNNNNNNGC 3 cut(s) 239, 470, 665
HpyF3I CTNAG 2 cut(s) 826, 895
HpySE526I ACGT 2 cut(s) 561, 1027
Hsp92II CATG 9 cut(s) 65, 241, 504, 574, 747, 776, 924, 940, 949
Ksp22I TGATCA 1 cut(s) 819
KspI CCGCGG 1 cut(s) 1455
Kzo9I GATC 3 cut(s) 451, 687, 819
LguI GCTCTTC 1 cut(s) 173
LmnI GCTCC 3 cut(s) 55, 326, 1259
Lsp1109I GCAGC 7 cut(s) 32, 136, 454, 457, 476, 646, 974
LweI GCATC 3 cut(s) 754, 1287, 1433
MaeI CTAG 1 cut(s) 639
MaeII ACGT 2 cut(s) 561, 1027
MaeIII GTNAC 3 cut(s) 259, 479, 1109
MalI GATC 3 cut(s) 453, 689, 821
MboI GATC 3 cut(s) 451, 687, 819
MboII GAAGA 7 cut(s) 23, 130, 160, 371, 499, 863, 1429
MfeI CAATTG 1 cut(s) 986
MflI RGATCY 1 cut(s) 451
MlsI TGGCCA 3 cut(s) 204, 242, 315
MluCI AATT 8 cut(s) 157, 405, 539, 777, 784, 986, 1135, 1341
MluI ACGCGT 1 cut(s) 693
MluNI TGGCCA 3 cut(s) 204, 242, 315
MlyI GAGTC 1 cut(s) 1002
MmeI TCCRAC 1 cut(s) 1123
Mox20I TGGCCA 3 cut(s) 204, 242, 315
MscI TGGCCA 3 cut(s) 204, 242, 315
MseI TTAA 2 cut(s) 915, 1251
Msp20I TGGCCA 3 cut(s) 204, 242, 315
MspA1I CMGCKG 5 cut(s) 23, 127, 467, 812, 1454
MspI CCGG 2 cut(s) 750, 1338
MspR9I CCNGG 1 cut(s) 751
MunI CAATTG 1 cut(s) 986
Mva1269I GAATGC 2 cut(s) 393, 950
MvnI CGCG 3 cut(s) 695, 1006, 1454
MwoI GCNNNNNNNGC 3 cut(s) 239, 470, 665
NciI CCSGG 1 cut(s) 751
NcoI CCATGG 3 cut(s) 237, 772, 945
NdeI CATATG 2 cut(s) 342, 1246
NdeII GATC 3 cut(s) 451, 687, 819
NlaIII CATG 9 cut(s) 65, 241, 504, 574, 747, 776, 924, 940, 949
NlaIV GGNNCC 1 cut(s) 567
NmuCI GTSAC 1 cut(s) 259
NspI RCATGY 1 cut(s) 924
NspV TTCGAA 1 cut(s) 843
PagI TCATGA 1 cut(s) 936
PaqCI CACCTGC 1 cut(s) 169
PciSI GCTCTTC 1 cut(s) 173
PctI GAATGC 2 cut(s) 393, 950
PfeI GAWTC 4 cut(s) 32, 65, 940, 1048
PkrI GCNGC 8 cut(s) 22, 126, 466, 469, 472, 661, 964, 1456
PleI GAGTC 1 cut(s) 1002
PpsI GAGTC 1 cut(s) 1002
Ppu21I YACGTR 1 cut(s) 1028
PshAI GACNNNNGTC 1 cut(s) 1192
PspFI CCCAGC 1 cut(s) 995
PspN4I GGNNCC 1 cut(s) 567
PspPI GGNCC 4 cut(s) 140, 747, 853, 1335
PstI CTGCAG 1 cut(s) 1291
PstNI CAGNNNCTG 2 cut(s) 263, 470
PsuI RGATCY 1 cut(s) 451
PvuII CAGCTG 4 cut(s) 23, 127, 467, 812
RsaI GTAC 2 cut(s) 1026, 1439
RsaNI GTAC 2 cut(s) 1025, 1438
SacII CCGCGG 1 cut(s) 1455
SapI GCTCTTC 1 cut(s) 173
SaqAI TTAA 2 cut(s) 915, 1251
SatI GCNGC 8 cut(s) 21, 125, 465, 468, 471, 660, 963, 1455
Sau3AI GATC 3 cut(s) 451, 687, 819
Sau96I GGNCC 4 cut(s) 140, 747, 853, 1335
SchI GAGTC 1 cut(s) 1002
ScrFI CCNGG 1 cut(s) 751
SfaNI GCATC 3 cut(s) 754, 1287, 1433
SfcI CTRYAG 2 cut(s) 255, 1287
Sfr303I CCGCGG 1 cut(s) 1455
SfuI TTCGAA 1 cut(s) 843
SgrBI CCGCGG 1 cut(s) 1455
SinI GGWCC 3 cut(s) 747, 853, 1335
SmlI CTYRAG 3 cut(s) 54, 434, 1052
SmoI CTYRAG 3 cut(s) 54, 434, 1052
Sse9I AATT 8 cut(s) 157, 405, 539, 777, 784, 986, 1135, 1341
SsiI CCGC 4 cut(s) 662, 1004, 1452, 1454
SspMI CTAG 1 cut(s) 639
StyD4I CCNGG 1 cut(s) 749
StyI CCWWGG 5 cut(s) 237, 643, 772, 945, 1315
TaaI ACNGT 5 cut(s) 259, 336, 600, 1075, 1348
TaiI ACGT 2 cut(s) 564, 1030
TaqI TCGA 2 cut(s) 843, 1090
TasI AATT 8 cut(s) 157, 405, 539, 777, 784, 986, 1135, 1341
TauI GCSGC 1 cut(s) 1457
TfiI GAWTC 4 cut(s) 32, 65, 940, 1048
Tru1I TTAA 2 cut(s) 915, 1251
Tru9I TTAA 2 cut(s) 915, 1251
TscAI CASTG 4 cut(s) 268, 603, 961, 1078
TseFI GTSAC 1 cut(s) 259
TseI GCWGC 7 cut(s) 20, 124, 464, 467, 470, 659, 962
Tsp45I GTSAC 1 cut(s) 259
TspDTI ATGAA 3 cut(s) 78, 237, 925
TspRI CASTG 4 cut(s) 268, 603, 961, 1078
VpaK11BI GGWCC 3 cut(s) 747, 853, 1335
XapI RAATTY 2 cut(s) 157, 539
XceI RCATGY 1 cut(s) 924
XcmI CCANNNNNNNNNTGG 1 cut(s) 1181
XmiI GTMKAC 1 cut(s) 630
XspI CTAG 1 cut(s) 639
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.