RLG00000008965

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0000521
Physical Location & Seq
Reverse (-)
43910257 .. 43913001
2745 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008965

Sequence Viewer

Length: 1482 bp
ATGGCTGACGAAGAACAATCTGCTGGTCTTGATTCACCCTTGATTCCAGTTCTGCCCCAAGAACATGCATTACAATCAACAAAGGGGCGATTCACGAAGGGCGAATTGTTGGGGGAACTGAAGAAGCAGTTATTGTTGGCAGGGCCGCTGGTATCATCAAATTTCTCGTTGTTCTGTATGGAGGTTATTTCAGTCATGTATGTTGGCCACCTTGGGGAGCTATCACTTGCAGGTGCTTCCATGGCCACTTCTTTTGCATCTGTGACTGGTTTGAGCTTGATAATAGGAATGGGTAGCGCATTAGACACATTTTGCGGGCAGTCGTATGGAGCAAAACAGTATCATATGCTTGGTGTACACATGCAGAGGGCAATGCTTGTTCTTCTGCTGGCCGGCATTCCTCTTGCAACTATATGGTTCAATGCAGGCCACATTCTCAAATTCTTGGGTCAAGATCCAGAAATTGCCGCTGCCGCTGGAAACTATGCTCGTTTCCTGATACCTTGCATTTTTGCTTACGCAGTCGAACAATGTCATTCCAGATTCTTGCAAACCCAGAACAATGTGGTTCCTATGATAGCTACCACAGGTATTGCAACACTAGTGCACTTGCTTCTCTGTTGGCTTCTGATATACAAGACCAGCTTGGGATATAGAGGCGCTGCTGTCGCAATCTCTATCTCATATTGGATCAATGCGTTACTCTTGGTTGTTTATATCAGAGTTTCTCCTTCTTGTATACACACATGGACTGGATTCTCAAAGGAGGCATTTCATGGAATTCCCAATTTCATAAGACTATCTATTCCTTCAGCTATAATGATCAGCCTAGAAATCTGGTCATTAGAAATGATGGTTCTCTTATCCGGTTTTCTTCCAAATCCACAGCTTGAAACCTCAGTGCTATCAATCAGCCTCAACACATGCTCCATGTTATACATGATTCCTCTTGCATTCGCCGGTGCAGCAAGCACAAGAGTTTCAAATCAATTGGGTGCCGGGCAACCACGACTAGCTCGTCTAGCAGTAAGTGTTGCACTATGCATTGTTGTTACTGAAGGTATTGTGGCTGCTGCAATACTGATACTGGGTCGAAAAGTTTGGGGCTACTGTTACAGCAGTGAAAAGGAAGTTGTCAGTTATGTTGGGCAAATGCTTATTTTGGTTGCGGTATCCCACTTTTTCGATGGACTTCAATCTGTGCTTTCAGGTATCATAAGAGGAAGTGGACAGCAGAAGATTGGAGCATATGTAAATCTGGGAGCTTATTATCTTATGGGAATCCCTACTGCATTATTATTAGCATTTGTATTCCACATTGGAGGAAAGGGTCTCTGGATGGGAATCATTGTTGCTCTATTTGTGCAAGCACTGTCTCTTGCAATCATAATCTTATTCACAGACTGGGAGAAAGAAGTGAAGAAAGCTTCTGATAGGGTATACAACACACCAACTGCGTCTAATGCATCATCGGTAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

494

Amino Acids

53.06

Weight (kDa)

8.29

Isoelectric Point (pI)

27.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 49 - 209 5.9e-39 MatE
MatE PF01554 270 - 431 1.7e-24 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000521)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g09990 FvH4_4g09990 FvH4_4g09990 FvH4_4g09990 FvH4_7g32860 FvH4_7g32860 FvH4_7g32860 FvH4_7g32860
malus_domestica MD11G1148000.v1.1 MD13G1266200.v1.1 MD14G1231800.v1.1
prunus_persica Prupe.1G090100_v2.0.a1 Prupe.1G090100_v2.0.a1 Prupe.1G090100_v2.0.a1 Prupe.1G090100_v2.0.a1 Prupe.1G090100_v2.0.a1 Prupe.1G090300_v2.0.a1 Prupe.1G090300_v2.0.a1 Prupe.1G090300_v2.0.a1 Prupe.1G090300_v2.0.a1 Prupe.1G090300_v2.0.a1
pyrus_communis pycom13g23330
rosa_chinensis RchiOBHm_Chr4g0403401 RchiOBHm_Chr4g0403411 RchiOBHm_Chr4g0403421 RchiOBHm_Chr4g0403461 RchiOBHm_Chr4g0403631 RchiOBHm_Chr7g0223601 RchiOBHm_Chr7g0238161
rosa_laevigata RLG00000000937 RLG00000001957 RLG00000008965 RLG00000008975 RLG00000008976 RLG00000008978
rosa_multiflora Rmu_co8335743.1_g000001 Rmu_sc0005734.1_g000001 Rmu_sc0007727.1_g000011 Rmu_sc0011775.1_g000002 Rmu_sc0011775.1_g000005 Rmu_sc0028062.1_g000002
rosa_roxburghii Rroxscaffold_3G00223300 Rroxscaffold_5G00348050 Rroxscaffold_5G00348060 Rroxscaffold_5G00348120 Rroxscaffold_5G00348240
rosa_rugosa Rorug04G0042300 Rorug04G0042400 Rorug04G0042500 Rorug04G0042600.1 Rorug04G0042700 Rorug04G0042800 Rorug04G0042900 Rorug04G0043000 Rorug04G0043100 Rorug04G0043200 Rorug04G0043300 Rorug04G0043400 Rorug04G0044700 Rorug07G0310600 Rorug07G0310700 Rorug07G0310700
rosa_samantha Rh4AG118700 Rh4AG118800 Rh4AG118900 Rh4AG119000 Rh4BG111600 Rh4BG111800 Rh4BG111900 Rh4BG112900 Rh4CG126600 Rh4CG126900 Rh4CG127800 Rh4DG111300 Rh4DG111400 Rh4DG111500 Rh7AG466500 Rh7BG352200 Rh7BG436900 Rh7CG484200 Rh7DG452600
rosa_wichuraiana Rw4G009630 Rw4G009640 Rw4G009650 Rw4G009710 Rw7G038620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 221
AasI GACNNNNNNGTC 1 cut(s) 1017
Acc36I ACCTGC 1 cut(s) 221
AccB1I GGYRCC 1 cut(s) 995
AccI GTMKAC 2 cut(s) 739, 1440
AciI CCGC 5 cut(s) 146, 315, 468, 474, 1169
AclWI GGATC 2 cut(s) 449, 698
AcoI YGGCCR 3 cut(s) 205, 243, 390
AcsI RAATTY 3 cut(s) 160, 440, 780
AcuI CTGAAG 3 cut(s) 140, 795, 1077
AfaI GTAC 1 cut(s) 357
AfiI CCNNNNNNNGG 1 cut(s) 214
AgsI TTSAA 4 cut(s) 421, 893, 984, 1196
AhlI ACTAGT 1 cut(s) 601
Alw21I GWGCWC 1 cut(s) 609
Alw26I GTCTC 2 cut(s) 1337, 1380
Alw44I GTGCAC 1 cut(s) 605
AlwI GGATC 2 cut(s) 449, 698
AoxI GGCC 5 cut(s) 143, 205, 243, 390, 427
ApaLI GTGCAC 1 cut(s) 605
ApeKI GCWGC 5 cut(s) 470, 662, 965, 1070, 1073
ApoI RAATTY 3 cut(s) 160, 440, 780
AspLEI GCGC 2 cut(s) 299, 662
AspS9I GGNCC 1 cut(s) 143
AsuC2I CCSGG 1 cut(s) 1000
AsuHPI GGTGA 1 cut(s) 27
BaeGI GKGCMC 1 cut(s) 609
BalI TGGCCA 2 cut(s) 207, 245
BanI GGYRCC 1 cut(s) 995
Bbv12I GWGCWC 1 cut(s) 609
BbvI GCAGC 5 cut(s) 457, 649, 977, 1057, 1060
BccI CCATC 3 cut(s) 847, 1181, 1333
BciVI GTATCC 1 cut(s) 1183
BclI TGATCA 1 cut(s) 822
BcnI CCSGG 1 cut(s) 1000
BcoDI GTCTC 2 cut(s) 1337, 1380
BcuI ACTAGT 1 cut(s) 601
BfaI CTAG 4 cut(s) 602, 830, 1013, 1022
BfoI RGCGCY 1 cut(s) 663
BfuAI ACCTGC 1 cut(s) 221
BfuI GTATCC 1 cut(s) 1183
BisI GCNGC 8 cut(s) 146, 468, 471, 474, 663, 966, 1071, 1074
BlsI GCNGC 8 cut(s) 147, 469, 472, 475, 664, 967, 1072, 1075
Bme1390I CCNGG 1 cut(s) 1000
BmgT120I GGNCC 1 cut(s) 143
BmiI GGNNCC 2 cut(s) 570, 997
BmrFI CCNGG 1 cut(s) 1000
BmrI ACTGGG 2 cut(s) 1097, 1414
BmsI GCATC 2 cut(s) 266, 1475
BmuI ACTGGG 2 cut(s) 1097, 1414
BpuMI CCSGG 1 cut(s) 1000
BsaBI GATNNNNATC 1 cut(s) 1343
BsaI GGTCTC 1 cut(s) 1337
BsaJI CCNNGG 2 cut(s) 211, 240
BsaWI WCCGGW 1 cut(s) 866
BsaXI ACNNNNNCTCC 4 cut(s) 911, 941, 1236, 1266
Bsc4I CCNNNNNNNGG 1 cut(s) 214
Bse118I RCCGGY 2 cut(s) 392, 959
Bse1I ACTGG 5 cut(s) 47, 271, 757, 1092, 1409
Bse3DI GCAATG 1 cut(s) 378
Bse8I GATNNNNATC 1 cut(s) 1343
BseDI CCNNGG 2 cut(s) 211, 240
BseGI GGATG 1 cut(s) 1344
BseJI GATNNNNATC 1 cut(s) 1343
BseLI CCNNNNNNNGG 1 cut(s) 214
BseMI GCAATG 1 cut(s) 378
BseMII CTCAG 1 cut(s) 912
BseNI ACTGG 5 cut(s) 47, 271, 757, 1092, 1409
BseSI GKGCMC 1 cut(s) 609
BseXI GCAGC 5 cut(s) 457, 649, 977, 1057, 1060
BsgI GTGCAG 1 cut(s) 984
BshFI GGCC 5 cut(s) 145, 207, 245, 392, 429
BshNI GGYRCC 1 cut(s) 995
BsiHKAI GWGCWC 1 cut(s) 609
BsiSI CCGG 4 cut(s) 393, 867, 960, 999
BslI CCNNNNNNNGG 1 cut(s) 214
BsmAI GTCTC 2 cut(s) 1337, 1380
BsmI GAATGC 2 cut(s) 396, 953
BsnI GGCC 5 cut(s) 145, 207, 245, 392, 429
Bso31I GGTCTC 1 cut(s) 1337
Bsp1286I GDGCHC 1 cut(s) 609
Bsp1407I TGTACA 1 cut(s) 355
Bsp143I GATC 3 cut(s) 454, 690, 822
Bsp19I CCATGG 1 cut(s) 240
BspACI CCGC 5 cut(s) 146, 315, 468, 474, 1169
BspANI GGCC 5 cut(s) 145, 207, 245, 392, 429
BspCNI CTCAG 1 cut(s) 911
BspLI GGNNCC 2 cut(s) 570, 997
BspMI ACCTGC 1 cut(s) 221
BspPI GGATC 2 cut(s) 449, 698
BspT107I GGYRCC 1 cut(s) 995
BspTNI GGTCTC 1 cut(s) 1337
BsrDI GCAATG 1 cut(s) 378
BsrFI RCCGGY 2 cut(s) 392, 959
BsrGI TGTACA 1 cut(s) 355
BsrI ACTGG 5 cut(s) 47, 271, 757, 1092, 1409
BssAI RCCGGY 2 cut(s) 392, 959
BssECI CCNNGG 2 cut(s) 211, 240
BssMI GATC 3 cut(s) 454, 690, 822
BssNAI GTATAC 2 cut(s) 740, 1441
BssT1I CCWWGG 2 cut(s) 211, 240
Bst1107I GTATAC 2 cut(s) 740, 1441
Bst4CI ACNGT 3 cut(s) 339, 1112, 1374
BstAUI TGTACA 1 cut(s) 355
BstC8I GCNNGC 6 cut(s) 317, 390, 394, 427, 970, 1368
BstDEI CTNAG 1 cut(s) 898
BstDSI CCRYGG 1 cut(s) 240
BstF5I GGATG 1 cut(s) 1344
BstH2I RGCGCY 1 cut(s) 663
BstHHI GCGC 2 cut(s) 299, 662
BstKTI GATC 3 cut(s) 457, 693, 825
BstMAI GTCTC 2 cut(s) 1337, 1380
BstMBI GATC 3 cut(s) 454, 690, 822
BstMWI GCNNNNNNNGC 6 cut(s) 242, 473, 668, 965, 1022, 1463
BstNSI RCATGY 3 cut(s) 68, 364, 927
BstSCI CCNGG 1 cut(s) 998
BstSLI GKGCMC 1 cut(s) 609
BstV1I GCAGC 5 cut(s) 457, 649, 977, 1057, 1060
BstX2I RGATCY 1 cut(s) 454
BstYI RGATCY 1 cut(s) 454
BstZ17I GTATAC 2 cut(s) 740, 1441
BsuI GTATCC 1 cut(s) 1183
BsuRI GGCC 5 cut(s) 145, 207, 245, 392, 429
BtgI CCRYGG 1 cut(s) 240
BtsCI GGATG 1 cut(s) 1344
BtsI GCAGTG 1 cut(s) 1126
BtsIMutI CAGTG 3 cut(s) 906, 1126, 1370
BveI ACCTGC 1 cut(s) 221
Cac8I GCNNGC 6 cut(s) 317, 390, 394, 427, 970, 1368
CfoI GCGC 2 cut(s) 299, 662
Cfr10I RCCGGY 2 cut(s) 392, 959
Cfr13I GGNCC 1 cut(s) 143
CseI GACGC 1 cut(s) 1446
Csp6I GTAC 1 cut(s) 356
CspCI CAANNNNNGTGG 4 cut(s) 235, 270, 574, 609
CviAII CATG 9 cut(s) 65, 196, 241, 361, 747, 776, 924, 931, 940
CviQI GTAC 1 cut(s) 356
DdeI CTNAG 1 cut(s) 898
DpnI GATC 3 cut(s) 456, 692, 824
DpnII GATC 3 cut(s) 454, 690, 822
DrdI GACNNNNNNGTC 1 cut(s) 1017
DseDI GACNNNNNNGTC 1 cut(s) 1017
EaeI YGGCCR 3 cut(s) 205, 243, 390
Eco130I CCWWGG 2 cut(s) 211, 240
Eco31I GGTCTC 1 cut(s) 1337
Eco57I CTGAAG 3 cut(s) 140, 795, 1077
EcoRI GAATTC 1 cut(s) 780
EcoT14I CCWWGG 2 cut(s) 211, 240
EcoT22I ATGCAT 3 cut(s) 70, 1046, 1468
ErhI CCWWGG 2 cut(s) 211, 240
FaeI CATG 9 cut(s) 68, 199, 244, 364, 750, 779, 927, 934, 943
FalI AAGNNNNNCTT 2 cut(s) 629, 661
FatI CATG 9 cut(s) 64, 195, 240, 360, 746, 775, 923, 930, 939
FauI CCCGC 1 cut(s) 308
FauNDI CATATG 2 cut(s) 345, 1249
FbaI TGATCA 1 cut(s) 822
FblI GTMKAC 2 cut(s) 739, 1440
Fnu4HI GCNGC 8 cut(s) 146, 468, 471, 474, 663, 966, 1071, 1074
FokI GGATG 1 cut(s) 1351
Fsp4HI GCNGC 8 cut(s) 146, 468, 471, 474, 663, 966, 1071, 1074
FspBI CTAG 4 cut(s) 602, 830, 1013, 1022
GlaI GCGC 2 cut(s) 298, 661
GluI GCNGC 8 cut(s) 146, 468, 471, 474, 663, 966, 1071, 1074
HaeII RGCGCY 1 cut(s) 663
HaeIII GGCC 5 cut(s) 145, 207, 245, 392, 429
HapII CCGG 4 cut(s) 393, 867, 960, 999
HgaI GACGC 1 cut(s) 1446
HhaI GCGC 2 cut(s) 299, 662
Hin1II CATG 9 cut(s) 68, 199, 244, 364, 750, 779, 927, 934, 943
Hin6I GCGC 2 cut(s) 297, 660
HinP1I GCGC 2 cut(s) 297, 660
HindIII AAGCTT 1 cut(s) 1425
HinfI GANTC 8 cut(s) 32, 43, 90, 543, 756, 943, 1281, 1344
HpaII CCGG 4 cut(s) 393, 867, 960, 999
HphI GGTGA 1 cut(s) 27
Hpy166II GTNNAC 6 cut(s) 356, 358, 607, 740, 1229, 1441
Hpy188I TCNGA 3 cut(s) 630, 722, 1432
Hpy188III TCNNGA 7 cut(s) 29, 94, 452, 458, 496, 540, 1336
Hpy8I GTNNAC 6 cut(s) 356, 358, 607, 740, 1229, 1441
HpyAV CCTTC 4 cut(s) 91, 741, 819, 1052
HpyCH4III ACNGT 3 cut(s) 339, 1112, 1374
HpyF10VI GCNNNNNNNGC 6 cut(s) 242, 473, 668, 965, 1022, 1463
HpyF3I CTNAG 1 cut(s) 898
Hsp92II CATG 9 cut(s) 68, 199, 244, 364, 750, 779, 927, 934, 943
HspAI GCGC 2 cut(s) 297, 660
KroI GCCGGC 1 cut(s) 392
KroNI GCCGGC 1 cut(s) 394
Ksp22I TGATCA 1 cut(s) 822
Kzo9I GATC 3 cut(s) 454, 690, 822
LmnI GCTCC 5 cut(s) 217, 329, 932, 1244, 1262
Lsp1109I GCAGC 5 cut(s) 457, 649, 977, 1057, 1060
LweI GCATC 2 cut(s) 266, 1475
MaeI CTAG 4 cut(s) 602, 830, 1013, 1022
MaeIII GTNAC 4 cut(s) 262, 699, 1051, 1112
MalI GATC 3 cut(s) 456, 692, 824
MboI GATC 3 cut(s) 454, 690, 822
MboII GAAGA 6 cut(s) 23, 133, 374, 866, 1249, 1432
MfeI CAATTG 1 cut(s) 989
MflI RGATCY 1 cut(s) 454
MhlI GDGCHC 1 cut(s) 609
MlsI TGGCCA 2 cut(s) 207, 245
MluCI AATT 7 cut(s) 104, 160, 440, 462, 780, 787, 989
MluNI TGGCCA 2 cut(s) 207, 245
Mox20I TGGCCA 2 cut(s) 207, 245
Mph1103I ATGCAT 3 cut(s) 70, 1046, 1468
MroNI GCCGGC 1 cut(s) 392
MscI TGGCCA 2 cut(s) 207, 245
Msp20I TGGCCA 2 cut(s) 207, 245
MspA1I CMGCKG 3 cut(s) 148, 470, 476
MspI CCGG 4 cut(s) 393, 867, 960, 999
MspR9I CCNGG 1 cut(s) 1000
MunI CAATTG 1 cut(s) 989
Mva1269I GAATGC 2 cut(s) 396, 953
MwoI GCNNNNNNNGC 6 cut(s) 242, 473, 668, 965, 1022, 1463
NaeI GCCGGC 1 cut(s) 394
NciI CCSGG 1 cut(s) 1000
NcoI CCATGG 1 cut(s) 240
NdeI CATATG 2 cut(s) 345, 1249
NdeII GATC 3 cut(s) 454, 690, 822
NgoMIV GCCGGC 1 cut(s) 392
NlaIII CATG 9 cut(s) 68, 199, 244, 364, 750, 779, 927, 934, 943
NlaIV GGNNCC 2 cut(s) 570, 997
NmuCI GTSAC 1 cut(s) 262
NsiI ATGCAT 3 cut(s) 70, 1046, 1468
NspI RCATGY 3 cut(s) 68, 364, 927
PaqCI CACCTGC 1 cut(s) 221
PcsI WCGNNNNNNNCGW 1 cut(s) 1015
PctI GAATGC 2 cut(s) 396, 953
PdiI GCCGGC 1 cut(s) 394
PfeI GAWTC 8 cut(s) 32, 43, 90, 543, 756, 943, 1281, 1344
PkrI GCNGC 8 cut(s) 147, 469, 472, 475, 664, 967, 1072, 1075
PspN4I GGNNCC 2 cut(s) 570, 997
PspPI GGNCC 1 cut(s) 143
PsrI GAACNNNNNNTAC 2 cut(s) 54, 86
PsuI RGATCY 1 cut(s) 454
RsaI GTAC 1 cut(s) 357
RsaNI GTAC 1 cut(s) 356
SatI GCNGC 8 cut(s) 146, 468, 471, 474, 663, 966, 1071, 1074
Sau3AI GATC 3 cut(s) 454, 690, 822
Sau96I GGNCC 1 cut(s) 143
ScrFI CCNGG 1 cut(s) 1000
SduI GDGCHC 1 cut(s) 609
SfaNI GCATC 2 cut(s) 266, 1475
SgrAI CRCCGGYG 1 cut(s) 959
SpeI ACTAGT 1 cut(s) 601
Sse9I AATT 7 cut(s) 104, 160, 440, 462, 780, 787, 989
SsiI CCGC 5 cut(s) 146, 315, 468, 474, 1169
SspMI CTAG 4 cut(s) 602, 830, 1013, 1022
StyD4I CCNGG 1 cut(s) 998
StyI CCWWGG 2 cut(s) 211, 240
TaaI ACNGT 3 cut(s) 339, 1112, 1374
TaqI TCGA 3 cut(s) 525, 1093, 1185
TasI AATT 7 cut(s) 104, 160, 440, 462, 780, 787, 989
TatI WGTACW 1 cut(s) 355
TauI GCSGC 3 cut(s) 148, 470, 476
TfiI GAWTC 8 cut(s) 32, 43, 90, 543, 756, 943, 1281, 1344
TscAI CASTG 3 cut(s) 906, 1126, 1377
TseFI GTSAC 1 cut(s) 262
TseI GCWGC 5 cut(s) 470, 662, 965, 1070, 1073
Tsp45I GTSAC 1 cut(s) 262
TspDTI ATGAA 2 cut(s) 764, 781
TspRI CASTG 3 cut(s) 906, 1126, 1377
VneI GTGCAC 1 cut(s) 605
XapI RAATTY 3 cut(s) 160, 440, 780
XceI RCATGY 3 cut(s) 68, 364, 927
XcmI CCANNNNNNNNNTGG 1 cut(s) 1184
XmiI GTMKAC 2 cut(s) 739, 1440
XspI CTAG 4 cut(s) 602, 830, 1013, 1022
Zsp2I ATGCAT 3 cut(s) 70, 1046, 1468
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.