Rorug04G0043200

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000521
Physical Location & Seq
Reverse (-)
6744674 .. 6767804
23131 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0043200.1

Sequence Viewer

Length: 3207 bp
ATGGGGACCGAAGCTTCAGCGAGACTTGTCAACTTTACTATTATAAGTATATGCCTCATCTTTACTCTTTCTCAGCTTGGTTCTGAATTCAAATATCAGAGTATAGCTCAAAAGTTGCCCGATGAAGAAGCGAGAGCTCTTAAGCGAGTAGTAGAGAAGTTGGAGTTAAACTATCGAATGTCCTCTAAAGGCTGCAATAAGAGCAGTAAGTATGACATCATATGTGACTGCAGTTTTCAAAACCACACCATCTGCCACGTCACAGAAATCTTTCACATTTTGAGTGACAGTGCACTGATGGCTAGCGGTTTGACTGGAATTATACCTGAAGAACTAGGCAGGCTCACACATCTGAGGGAACTGGATCTCAATATCAATCAATTGACTGGTTCTATACCCGCTAGCTTGGGAAATTTACCTTCGCTTTACAAGCTGAGTCTTTACAAAAATCAATTGACCGGGCCCATTCCAGCGAGTTTGGGAAATATGAAATTTCAAGTCCGCATGGATCATACTTCGCCCCGCAGCATGGCTGTTAGCTTCGAACTGGATCTTTCATACAATCAATTAAGTGGACCCATACCAGCCACTTTAAGGAATGTGCAGCCTGTGGCATTTGATCCAGATGCCTACCGACTCTTCAAGCTTAAGCAATACAACAGGGCTAATGTTAACCCAGATGATTTCTACATGGATCTTTCAAGCAATCAACTGACTGGGCCTATACCAGAGAGCTTAGGGAATTTGACTTTCCTCAGTTCTATTGATCTGGCGAACAACTTTCTCGACGGCTATATACCAGCATCTTTGGGTGCACTGACTTATCTGCGTGAATTGTATTTGGATAACAATAGAATCTCTGGTACTCTCCCACAAACACTAGGAAATTTGACACTCCTTGAAATCTTCGTGGTCGGATCTAACAGTATCACTGGGACATTACCAAAAAGCCTTGCCAGCCTTAAGAGTCTGTCTGCATTTTTAATAGCTGGTAACTATTTTTCTGTCCCTTTACCTGACTTCATAGCCAACTGGACTTCAATCGCGTATCTTTTTCTCAACGGAAACAATTTCAACGGGAAGATACCTGCCGGAATTTTTAATTTACGACACCTAGAAATGTTGATACTAAGAAATTGCTCAGTCACGGGTCAAATTCCTTCTTACATTGGAGATATGTCATCATTAAAGAACATAGACTTGAGCTTCAACAGGTTAACGGGTAGAATCCCAGATTCTTTGAAAAACTTAAATTTGAGTTACATGTCTTTTTCAAACAATACGCTTTCTGGGGAAATTCCTGATTGGATTCAAAATGCAAAATGGAGTAAGATGGATTTTTCATACAATAATTTTTCAAAGCTAACCTTTAAACCGTCACACAATCTACAACTGAATTTGTTTTCCTGCTGCTGCAACTCCTCAACCTGTCTGCCAAATACGACGGACCCAACCAAGGAGAAGTATTGTCCCCAGGGAAAACCTAATTACCATTCATTGTTCATAAATTGTGGTGGTGGCAAAACAACTTTTGGTGGAAATGATTATGATCAAGATAATGATACGTCACTATTTCACCTGAGTCTAAAAGGAAACTGGGCTCGGAGTAGCGTTGGAAACACCATCGAGGATGTTACCAATGGCAATTCTAGTAAATTGTTAAAAAGCGTTAGCTGTGGGCTTTCATCTGAAGAAAATTTATACGATAAAGCTCGCATTTCCCTTGTCTCTCTAAAATATTACGGGTTCTGTTTACGTAAAGGCACATACAAAGTAAAACTTCATTTTGCTGAAATTGTTGATGAGTATGCAGATGTTCATGAGGATGTTGATTTTCGAGATACAGATAAACGCGCATTTGATGTATATATTCAGGGTGAGATAAAACTAAAGGATTTCAACATTATAGACAAGGCAGGACGTCGGAATGCAGAACATACAGAAAATTTCATTGTTGATGTTACTGACAGTACATTAGAGATCCACTTATACTCTCGATCGGCTGGAGAAGGGACACTTGATGGACCTCTGATATCTGCTATATCCGTAACCCAAGAGTACAAAAAGCAACAACTATCTCCTTTGCAAACAGCACTCATTACTGTGGCTTCAATCATAGTTTTATTGTTGCTTTTATTACTTTTGGGGAATACAGATCACCTACAAGAAATAGATATAGGTATAGAAAAGCCTGTCACCCTCAAACAATTAAAAGATGCTACTCGGAATTTTAGCAAGAGGAATGAGATTGCTCAAGGGGGTTTTGGGACCGTTTACAAGGCCGAAGTGCAAGGGAAAATTGTAGCCGTGAAGAAAATTTGCTCTCATTCAGAGGAAAGGATCAATGAGTTCATAAATGAATTTTATACCTTAAAATCAATGAGTCAAGAGAACCTTGTTCAGTTGCTGGACATTTACAACGCAAAAGGCCTGCATTTGCTCATCTATGAATATATGGAGAACAACTCCCTTGCACATGCCTTATTTGACTCCAAGTCCAAATTGAAACTTGATTGGGAAGTTAGATTTAACATTTGCTTGGGAATAGCTAGGGGATTGGAGTATCTACATGAGCATCCCAGGATGAAAATTGTTCATAGCGACATTAAATCCGCTAATATTCTTCTTGATGGAAACCTTAAGGCTAAAATATCAGACTTTGGATTGGCAAGACTTTACACCGAAGGTGATCAATTCAAGTTCATCAAAGTAGAAGTGCCACAAGGATATATGGCACCTGAGTATGTTCGAGGAATTGTGACATCTAAAGCTGATGTCTACAGTTTTGGAGTGGTTATACTTGAAACTGTTAGTGGAAGGACAAATGCAGGACACAGGCAAGATAGCCAGGAAAGTGAATTTCTTCTAGACACGGCTTATGATTTACATCGAAAAGGAAGGCTGGTGGACTTGGTTGACAAAACCTTGTCTAACAAGTATGATGCAAAACAAGCCATAATCATTTTGAATTTAGCAGTAAAGTGCACCAGTATATCTCCAACTCTGAGGCCTACTATGTCTGAAGTAGTGAGTGTTCTCGTTGGCGACAAAAAAATTGAGGAGATTTGTCTCCCTGCTCTTAATGATAGTCACCTTGCTCAAGTTGATTCCTCTGTTTCTATGGAAGCAACCTCGAGAGCATCCACATCATCAAATTTGATCAAAGGGGAAGATGAAACAGAACACATTTCTGAGAGCAACCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

1068

Amino Acids

118.95

Weight (kDa)

6.1

Isoelectric Point (pI)

34.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 230 - 325 2.5e-06 Leucine-rich repeat region
LRR_8 PF13855 275 - 333 8.6e-07 Leucine rich repeat
Malectin PF11721 499 - 681 4e-27 Malectin domain
Pkinase PF00069 745 - 1010 1.1e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 747 - 1013 2.2e-42 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000521)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g09990 FvH4_4g09990 FvH4_4g09990 FvH4_4g09990 FvH4_7g32860 FvH4_7g32860 FvH4_7g32860 FvH4_7g32860
malus_domestica MD11G1148000.v1.1 MD13G1266200.v1.1 MD14G1231800.v1.1
prunus_persica Prupe.1G090100_v2.0.a1 Prupe.1G090100_v2.0.a1 Prupe.1G090100_v2.0.a1 Prupe.1G090100_v2.0.a1 Prupe.1G090100_v2.0.a1 Prupe.1G090300_v2.0.a1 Prupe.1G090300_v2.0.a1 Prupe.1G090300_v2.0.a1 Prupe.1G090300_v2.0.a1 Prupe.1G090300_v2.0.a1
pyrus_communis pycom13g23330
rosa_chinensis RchiOBHm_Chr4g0403401 RchiOBHm_Chr4g0403411 RchiOBHm_Chr4g0403421 RchiOBHm_Chr4g0403461 RchiOBHm_Chr4g0403631 RchiOBHm_Chr7g0223601 RchiOBHm_Chr7g0238161
rosa_laevigata RLG00000000937 RLG00000001957 RLG00000008965 RLG00000008975 RLG00000008976 RLG00000008978
rosa_multiflora Rmu_co8335743.1_g000001 Rmu_sc0005734.1_g000001 Rmu_sc0007727.1_g000011 Rmu_sc0011775.1_g000002 Rmu_sc0011775.1_g000005 Rmu_sc0028062.1_g000002
rosa_roxburghii Rroxscaffold_3G00223300 Rroxscaffold_5G00348050 Rroxscaffold_5G00348060 Rroxscaffold_5G00348120 Rroxscaffold_5G00348240
rosa_rugosa Rorug04G0042300 Rorug04G0042400 Rorug04G0042500 Rorug04G0042600.1 Rorug04G0042700 Rorug04G0042800 Rorug04G0042900 Rorug04G0043000 Rorug04G0043100 Rorug04G0043200 Rorug04G0043300 Rorug04G0043400 Rorug04G0044700 Rorug07G0310600 Rorug07G0310700 Rorug07G0310700
rosa_samantha Rh4AG118700 Rh4AG118800 Rh4AG118900 Rh4AG119000 Rh4BG111600 Rh4BG111800 Rh4BG111900 Rh4BG112900 Rh4CG126600 Rh4CG126900 Rh4CG127800 Rh4DG111300 Rh4DG111400 Rh4DG111500 Rh7AG466500 Rh7BG352200 Rh7BG436900 Rh7CG484200 Rh7DG452600
rosa_wichuraiana Rw4G009630 Rw4G009640 Rw4G009650 Rw4G009710 Rw7G038620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 44
AatII GACGTC 1 cut(s) 1924
Acc36I ACCTGC 1 cut(s) 1096
AccB1I GGYRCC 1 cut(s) 2734
AccI GTMKAC 1 cut(s) 2778
AccII CGCG 2 cut(s) 1046, 1854
AciI CCGC 5 cut(s) 306, 399, 502, 523, 2613
AclWI GGATC 8 cut(s) 372, 516, 558, 614, 702, 925, 1975, 2348
AcuI CTGAAG 3 cut(s) 348, 1710, 3042
AcyI GRCGYC 1 cut(s) 1921
AfaI GTAC 3 cut(s) 865, 1972, 2060
AfiI CCNNNNNNNGG 3 cut(s) 529, 594, 1456
AflII CTTAAG 4 cut(s) 140, 647, 962, 2639
AflIII ACRYGT 1 cut(s) 1263
AhdI GACNNNNNGTC 1 cut(s) 2494
AjiI CACGTC 1 cut(s) 259
AjnI CCWGG 3 cut(s) 1473, 2579, 2847
Alw21I GWGCWC 4 cut(s) 139, 295, 817, 2987
Alw26I GTCTC 3 cut(s) 16, 1732, 3074
Alw44I GTGCAC 3 cut(s) 291, 813, 2983
AlwI GGATC 8 cut(s) 372, 516, 558, 614, 702, 925, 1975, 2348
AlwNI CAGNNNCTG 1 cut(s) 2407
Ama87I CYCGRG 1 cut(s) 3133
AoxI GGCC 5 cut(s) 461, 719, 2280, 2428, 3008
ApaI GGGCCC 1 cut(s) 465
ApaLI GTGCAC 3 cut(s) 291, 813, 2983
ApeKI GCWGC 5 cut(s) 192, 525, 604, 1410, 1413
Asp700I GAANNNNTTC 2 cut(s) 270, 2862
AspLEI GCGC 1 cut(s) 1856
AspS9I GGNCC 8 cut(s) 6, 461, 462, 575, 719, 1447, 2024, 2268
AsuC2I CCSGG 1 cut(s) 460
AsuHPI GGTGA 6 cut(s) 1568, 1889, 2150, 2188, 2699, 3083
AsuII TTCGAA 1 cut(s) 543
AsuNHI GCTAGC 2 cut(s) 302, 401
AvaI CYCGRG 1 cut(s) 3133
AvaII GGWCC 5 cut(s) 6, 575, 1447, 2024, 2268
BaeGI GKGCMC 4 cut(s) 295, 465, 817, 2987
BanI GGYRCC 1 cut(s) 2734
BanII GRGCYC 3 cut(s) 139, 465, 1603
Bbv12I GWGCWC 4 cut(s) 139, 295, 817, 2987
BbvI GCAGC 5 cut(s) 179, 537, 616, 1397, 1400
BccI CCATC 6 cut(s) 257, 292, 1327, 1631, 2015, 2624
BceAI ACGGC 3 cut(s) 805, 2291, 2889
BciT130I CCWGG 3 cut(s) 1475, 2581, 2849
BclI TGATCA 3 cut(s) 1549, 2689, 3159
BcnI CCSGG 1 cut(s) 460
BcoDI GTCTC 3 cut(s) 16, 1732, 3074
BfaI CTAG 9 cut(s) 303, 335, 402, 881, 1115, 1650, 2550, 2867, 3205
BfmI CTRYAG 2 cut(s) 229, 2779
BfrI CTTAAG 4 cut(s) 140, 647, 962, 2639
BfuAI ACCTGC 1 cut(s) 1096
BisI GCNGC 5 cut(s) 193, 526, 605, 1411, 1414
BlsI GCNGC 5 cut(s) 194, 527, 606, 1412, 1415
Bme1390I CCNGG 4 cut(s) 460, 1475, 2581, 2849
Bme18I GGWCC 5 cut(s) 6, 575, 1447, 2024, 2268
BmeRI GACNNNNNGTC 1 cut(s) 2494
BmeT110I CYCGRG 1 cut(s) 3133
BmgBI CACGTC 1 cut(s) 259
BmgT120I GGNCC 8 cut(s) 6, 461, 462, 575, 719, 1447, 2024, 2268
BmiI GGNNCC 6 cut(s) 7, 463, 577, 1449, 2269, 2736
BmrFI CCNGG 4 cut(s) 460, 1475, 2581, 2849
BmrI ACTGGG 3 cut(s) 726, 942, 1606
BmsI GCATC 6 cut(s) 616, 812, 2206, 2584, 2932, 3149
BmtI GCTAGC 2 cut(s) 306, 405
BmuI ACTGGG 3 cut(s) 726, 942, 1606
BplI GAGNNNNNCTC 4 cut(s) 91, 123, 2450, 2482
BpmI CTGGAG 1 cut(s) 2025
Bpu10I CCTNAGC 1 cut(s) 736
Bpu14I TTCGAA 1 cut(s) 543
BpuEI CTTGAG 3 cut(s) 1222, 2238, 3084
BpuMI CCSGG 1 cut(s) 460
BsaAI YACGTR 1 cut(s) 1757
BsaBI GATNNNNATC 2 cut(s) 1548, 2886
BsaHI GRCGYC 1 cut(s) 1921
BsaJI CCNNGG 4 cut(s) 1455, 1473, 1474, 2579
Bsc4I CCNNNNNNNGG 3 cut(s) 529, 594, 1456
Bse1I ACTGG 9 cut(s) 319, 366, 391, 552, 721, 937, 1037, 1601, 2988
Bse8I GATNNNNATC 2 cut(s) 1548, 2886
BseBI CCWGG 3 cut(s) 1475, 2581, 2849
BseDI CCNNGG 4 cut(s) 1455, 1473, 1474, 2579
BseGI GGATG 5 cut(s) 1636, 1831, 2575, 2589, 3140
BseJI GATNNNNATC 2 cut(s) 1548, 2886
BseLI CCNNNNNNNGG 3 cut(s) 529, 594, 1456
BseMII CTCAG 9 cut(s) 86, 344, 425, 769, 1155, 1571, 2730, 2996, 3183
BseNI ACTGG 9 cut(s) 319, 366, 391, 552, 721, 937, 1037, 1601, 2988
BseRI GAGGAG 2 cut(s) 1411, 3074
BseSI GKGCMC 4 cut(s) 295, 465, 817, 2987
BseXI GCAGC 5 cut(s) 179, 537, 616, 1397, 1400
BsgI GTGCAG 1 cut(s) 623
Bsh1236I CGCG 2 cut(s) 1046, 1854
Bsh1285I CGRYCG 1 cut(s) 2000
BshFI GGCC 5 cut(s) 463, 721, 2282, 2430, 3010
BshNI GGYRCC 1 cut(s) 2734
BsiEI CGRYCG 1 cut(s) 2000
BsiHKAI GWGCWC 4 cut(s) 139, 295, 817, 2987
BsiHKCI CYCGRG 1 cut(s) 3133
BsiSI CCGG 2 cut(s) 459, 1092
BslFI GGGAC 6 cut(s) 19, 949, 992, 1455, 2026, 2281
BslI CCNNNNNNNGG 3 cut(s) 529, 594, 1456
BsmAI GTCTC 3 cut(s) 16, 1732, 3074
BsmFI GGGAC 6 cut(s) 19, 949, 992, 1455, 2026, 2281
BsmI GAATGC 1 cut(s) 1933
BsnI GGCC 5 cut(s) 463, 721, 2282, 2430, 3010
BsoBI CYCGRG 1 cut(s) 3133
Bsp119I TTCGAA 1 cut(s) 543
Bsp120I GGGCCC 1 cut(s) 461
Bsp1286I GDGCHC 6 cut(s) 139, 295, 465, 817, 1603, 2987
BspACI CCGC 5 cut(s) 306, 399, 502, 523, 2613
BspANI GGCC 5 cut(s) 463, 721, 2282, 2430, 3010
BspCNI CTCAG 9 cut(s) 85, 345, 426, 768, 1154, 1572, 2731, 2997, 3184
BspFNI CGCG 2 cut(s) 1046, 1854
BspHI TCATGA 1 cut(s) 1819
BspLI GGNNCC 6 cut(s) 7, 463, 577, 1449, 2269, 2736
BspMAI CTGCAG 1 cut(s) 233
BspMI ACCTGC 1 cut(s) 1096
BspOI GCTAGC 2 cut(s) 306, 405
BspPI GGATC 8 cut(s) 372, 516, 558, 614, 702, 925, 1975, 2348
BspT104I TTCGAA 1 cut(s) 543
BspT107I GGYRCC 1 cut(s) 2734
BspTI CTTAAG 4 cut(s) 140, 647, 962, 2639
BsrI ACTGG 9 cut(s) 319, 366, 391, 552, 721, 937, 1037, 1601, 2988
BssECI CCNNGG 4 cut(s) 1455, 1473, 1474, 2579
BssNI GRCGYC 1 cut(s) 1921
BssT1I CCWWGG 1 cut(s) 1455
Bst2UI CCWGG 3 cut(s) 1475, 2581, 2849
Bst4CI ACNGT 8 cut(s) 290, 926, 1377, 1970, 2104, 2272, 2783, 2809
Bst6I CTCTTC 1 cut(s) 644
BstACI GRCGYC 1 cut(s) 1921
BstAFI CTTAAG 4 cut(s) 140, 647, 962, 2639
BstBAI YACGTR 1 cut(s) 1757
BstBI TTCGAA 1 cut(s) 543
BstC8I GCNNGC 6 cut(s) 304, 341, 403, 958, 1714, 2432
BstF5I GGATG 5 cut(s) 1636, 1831, 2575, 2589, 3140
BstFNI CGCG 2 cut(s) 1046, 1854
BstHHI GCGC 1 cut(s) 1856
BstMAI GTCTC 3 cut(s) 16, 1732, 3074
BstMCI CGRYCG 1 cut(s) 2000
BstMWI GCNNNNNNNGC 5 cut(s) 201, 299, 430, 957, 2951
BstNI CCWGG 3 cut(s) 1475, 2581, 2849
BstNSI RCATGY 2 cut(s) 1267, 2480
BstSCI CCNGG 4 cut(s) 458, 1473, 2579, 2847
BstSFI CTRYAG 2 cut(s) 229, 2779
BstSLI GKGCMC 4 cut(s) 295, 465, 817, 2987
BstSNI TACGTA 1 cut(s) 1757
BstUI CGCG 2 cut(s) 1046, 1854
BstV1I GCAGC 5 cut(s) 179, 537, 616, 1397, 1400
BstX2I RGATCY 5 cut(s) 364, 550, 694, 917, 1980
BstYI RGATCY 5 cut(s) 364, 550, 694, 917, 1980
BsuRI GGCC 5 cut(s) 463, 721, 2282, 2430, 3010
BtrI CACGTC 1 cut(s) 259
BtsCI GGATG 5 cut(s) 1636, 1831, 2575, 2589, 3140
BtsIMutI CAGTG 4 cut(s) 293, 295, 815, 930
BveI ACCTGC 1 cut(s) 1096
Cac8I GCNNGC 6 cut(s) 304, 341, 403, 958, 1714, 2432
CaiI CAGNNNCTG 1 cut(s) 2407
CciI TCATGA 1 cut(s) 1819
CfoI GCGC 1 cut(s) 1856
Cfr13I GGNCC 8 cut(s) 6, 461, 462, 575, 719, 1447, 2024, 2268
Csp6I GTAC 3 cut(s) 864, 1971, 2059
CviAII CATG 7 cut(s) 505, 529, 691, 1264, 1820, 2477, 2570
CviQI GTAC 3 cut(s) 864, 1971, 2059
DraI TTTAAA 1 cut(s) 1372
DriI GACNNNNNGTC 1 cut(s) 2494
Eam1104I CTCTTC 1 cut(s) 644
Eam1105I GACNNNNNGTC 1 cut(s) 2494
EarI CTCTTC 1 cut(s) 644
Ecl136II GAGCTC 1 cut(s) 137
Eco105I TACGTA 1 cut(s) 1757
Eco130I CCWWGG 1 cut(s) 1455
Eco147I AGGCCT 2 cut(s) 2430, 3010
Eco24I GRGCYC 3 cut(s) 139, 465, 1603
Eco32I GATATC 1 cut(s) 2034
Eco47I GGWCC 5 cut(s) 6, 575, 1447, 2024, 2268
Eco53kI GAGCTC 1 cut(s) 137
Eco57I CTGAAG 3 cut(s) 348, 1710, 3042
Eco88I CYCGRG 1 cut(s) 3133
EcoICRI GAGCTC 1 cut(s) 137
EcoRI GAATTC 1 cut(s) 86
EcoRII CCWGG 3 cut(s) 1473, 2579, 2847
EcoRV GATATC 1 cut(s) 2034
EcoT14I CCWWGG 1 cut(s) 1455
EcoT38I GRGCYC 3 cut(s) 139, 465, 1603
ErhI CCWWGG 1 cut(s) 1455
FaeI CATG 7 cut(s) 508, 532, 694, 1267, 1823, 2480, 2573
FalI AAGNNNNNCTT 8 cut(s) 1352, 1384, 1764, 1796, 2001, 2033, 2379, 2411
FaqI GGGAC 6 cut(s) 19, 949, 992, 1455, 2026, 2281
FatI CATG 7 cut(s) 504, 528, 690, 1263, 1819, 2476, 2569
FauI CCCGC 2 cut(s) 406, 530
FauNDI CATATG 1 cut(s) 221
FbaI TGATCA 3 cut(s) 1549, 2689, 3159
FblI GTMKAC 1 cut(s) 2778
Fnu4HI GCNGC 5 cut(s) 193, 526, 605, 1411, 1414
FokI GGATG 5 cut(s) 1643, 1838, 2562, 2596, 3127
FriOI GRGCYC 3 cut(s) 139, 465, 1603
Fsp4HI GCNGC 5 cut(s) 193, 526, 605, 1411, 1414
FspBI CTAG 9 cut(s) 303, 335, 402, 881, 1115, 1650, 2550, 2867, 3205
GlaI GCGC 1 cut(s) 1855
GluI GCNGC 5 cut(s) 193, 526, 605, 1411, 1414
GsuI CTGGAG 1 cut(s) 2025
HaeIII GGCC 5 cut(s) 463, 721, 2282, 2430, 3010
HapII CCGG 2 cut(s) 459, 1092
HhaI GCGC 1 cut(s) 1856
Hin1I GRCGYC 1 cut(s) 1921
Hin1II CATG 7 cut(s) 508, 532, 694, 1267, 1823, 2480, 2573
Hin6I GCGC 1 cut(s) 1854
HinP1I GCGC 1 cut(s) 1854
HincII GTYRAC 4 cut(s) 31, 673, 1218, 2917
HindII GTYRAC 4 cut(s) 31, 673, 1218, 2917
HindIII AAGCTT 2 cut(s) 12, 644
HpaI GTTAAC 2 cut(s) 673, 1218
HpaII CCGG 2 cut(s) 459, 1092
HphI GGTGA 6 cut(s) 1568, 1889, 2150, 2188, 2699, 3083
Hpy99I CGWCG 3 cut(s) 791, 1447, 1926
HpyAV CCTTC 6 cut(s) 429, 1170, 2003, 2678, 2811, 2892
HpyCH4III ACNGT 8 cut(s) 290, 926, 1377, 1970, 2104, 2272, 2783, 2809
HpyCH4IV ACGT 4 cut(s) 258, 1565, 1756, 1921
HpyF10VI GCNNNNNNNGC 5 cut(s) 201, 299, 430, 957, 2951
HpySE526I ACGT 4 cut(s) 258, 1565, 1756, 1921
Hsp92I GRCGYC 1 cut(s) 1921
Hsp92II CATG 7 cut(s) 508, 532, 694, 1267, 1823, 2480, 2573
HspAI GCGC 1 cut(s) 1854
Ksp22I TGATCA 3 cut(s) 1549, 2689, 3159
KspAI GTTAAC 2 cut(s) 673, 1218
Lsp1109I GCAGC 5 cut(s) 179, 537, 616, 1397, 1400
LweI GCATC 6 cut(s) 616, 812, 2206, 2584, 2932, 3149
MaeI CTAG 9 cut(s) 303, 335, 402, 881, 1115, 1650, 2550, 2867, 3205
MaeII ACGT 4 cut(s) 258, 1565, 1756, 1921
MfeI CAATTG 2 cut(s) 380, 452
MflI RGATCY 5 cut(s) 364, 550, 694, 917, 1980
MhlI GDGCHC 6 cut(s) 139, 295, 465, 817, 1603, 2987
MlyI GAGTC 6 cut(s) 445, 630, 976, 1591, 2392, 2483
MmeI TCCRAC 5 cut(s) 141, 895, 1594, 1904, 3023
MroXI GAANNNNTTC 2 cut(s) 270, 2862
MslI CAYNNNNRTG 2 cut(s) 1824, 2102
MspCI CTTAAG 4 cut(s) 140, 647, 962, 2639
MspI CCGG 2 cut(s) 459, 1092
MspR9I CCNGG 4 cut(s) 460, 1475, 2581, 2849
MunI CAATTG 2 cut(s) 380, 452
Mva1269I GAATGC 1 cut(s) 1933
MvaI CCWGG 3 cut(s) 1475, 2581, 2849
MvnI CGCG 2 cut(s) 1046, 1854
MwoI GCNNNNNNNGC 5 cut(s) 201, 299, 430, 957, 2951
NciI CCSGG 1 cut(s) 460
NdeI CATATG 1 cut(s) 221
NheI GCTAGC 2 cut(s) 302, 401
NlaIII CATG 7 cut(s) 508, 532, 694, 1267, 1823, 2480, 2573
NlaIV GGNNCC 6 cut(s) 7, 463, 577, 1449, 2269, 2736
NmuCI GTSAC 9 cut(s) 224, 259, 284, 1144, 1377, 1566, 2194, 2758, 3089
NspI RCATGY 2 cut(s) 1267, 2480
NspV TTCGAA 1 cut(s) 543
PaeR7I CTCGAG 1 cut(s) 3133
PagI TCATGA 1 cut(s) 1819
PasI CCCWGGG 1 cut(s) 1474
PceI AGGCCT 2 cut(s) 2430, 3010
PciI ACATGT 1 cut(s) 1263
PctI GAATGC 1 cut(s) 1933
PdmI GAANNNNTTC 2 cut(s) 270, 2862
PfeI GAWTC 5 cut(s) 855, 1227, 1235, 1309, 3107
PkrI GCNGC 5 cut(s) 194, 527, 606, 1412, 1415
Ple19I CGATCG 1 cut(s) 2000
PleI GAGTC 6 cut(s) 444, 630, 975, 1590, 2391, 2483
PpsI GAGTC 6 cut(s) 444, 630, 975, 1590, 2391, 2483
Ppu21I YACGTR 1 cut(s) 1757
PscI ACATGT 1 cut(s) 1263
PsiI TTATAA 1 cut(s) 44
Psp124BI GAGCTC 1 cut(s) 139
Psp6I CCWGG 3 cut(s) 1473, 2579, 2847
PspGI CCWGG 3 cut(s) 1473, 2579, 2847
PspN4I GGNNCC 6 cut(s) 7, 463, 577, 1449, 2269, 2736
PspOMI GGGCCC 1 cut(s) 461
PspPI GGNCC 8 cut(s) 6, 461, 462, 575, 719, 1447, 2024, 2268
PsrI GAACNNNNNNTAC 2 cut(s) 3018, 3050
PstI CTGCAG 1 cut(s) 233
PstNI CAGNNNCTG 1 cut(s) 2407
PsuI RGATCY 5 cut(s) 364, 550, 694, 917, 1980
PvuI CGATCG 1 cut(s) 2000
RsaI GTAC 3 cut(s) 865, 1972, 2060
RsaNI GTAC 3 cut(s) 864, 1971, 2059
RseI CAYNNNNRTG 2 cut(s) 1824, 2102
SacI GAGCTC 1 cut(s) 139
SatI GCNGC 5 cut(s) 193, 526, 605, 1411, 1414
Sau96I GGNCC 8 cut(s) 6, 461, 462, 575, 719, 1447, 2024, 2268
SchI GAGTC 6 cut(s) 445, 630, 976, 1591, 2392, 2483
ScrFI CCNGG 4 cut(s) 460, 1475, 2581, 2849
SduI GDGCHC 6 cut(s) 139, 295, 465, 817, 1603, 2987
SfaNI GCATC 6 cut(s) 616, 812, 2206, 2584, 2932, 3149
SfcI CTRYAG 2 cut(s) 229, 2779
Sfr274I CTCGAG 1 cut(s) 3133
SfuI TTCGAA 1 cut(s) 543
SinI GGWCC 5 cut(s) 6, 575, 1447, 2024, 2268
SlaI CTCGAG 1 cut(s) 3133
SmiMI CAYNNNNRTG 2 cut(s) 1824, 2102
SmlI CTYRAG 8 cut(s) 140, 647, 962, 1201, 2253, 2639, 3099, 3133
SmoI CTYRAG 8 cut(s) 140, 647, 962, 1201, 2253, 2639, 3099, 3133
SnaBI TACGTA 1 cut(s) 1757
SseBI AGGCCT 2 cut(s) 2430, 3010
SsiI CCGC 5 cut(s) 306, 399, 502, 523, 2613
SspI AATATT 2 cut(s) 1739, 2620
SspMI CTAG 9 cut(s) 303, 335, 402, 881, 1115, 1650, 2550, 2867, 3205
SstI GAGCTC 1 cut(s) 139
StuI AGGCCT 2 cut(s) 2430, 3010
StyD4I CCNGG 4 cut(s) 458, 1473, 2579, 2847
StyI CCWWGG 1 cut(s) 1455
TaaI ACNGT 8 cut(s) 290, 926, 1377, 1970, 2104, 2272, 2783, 2809
TaiI ACGT 4 cut(s) 261, 1568, 1759, 1924
TaqI TCGA 9 cut(s) 175, 543, 786, 1626, 1837, 1996, 2749, 2890, 3134
TaqII GACCGA 1 cut(s) 23
TatI WGTACW 2 cut(s) 1970, 2058
TfiI GAWTC 5 cut(s) 855, 1227, 1235, 1309, 3107
TscAI CASTG 4 cut(s) 295, 300, 822, 937
TseFI GTSAC 9 cut(s) 224, 259, 284, 1144, 1377, 1566, 2194, 2758, 3089
TseI GCWGC 5 cut(s) 192, 525, 604, 1410, 1413
Tsp45I GTSAC 9 cut(s) 224, 259, 284, 1144, 1377, 1566, 2194, 2758, 3089
TspGWI ACGGA 3 cut(s) 1077, 1460, 2035
TspRI CASTG 4 cut(s) 295, 300, 822, 937
Vha464I CTTAAG 4 cut(s) 140, 647, 962, 2639
VneI GTGCAC 3 cut(s) 291, 813, 2983
VpaK11BI GGWCC 5 cut(s) 6, 575, 1447, 2024, 2268
XbaI TCTAGA 1 cut(s) 2866
XceI RCATGY 2 cut(s) 1267, 2480
XhoI CTCGAG 1 cut(s) 3133
XmiI GTMKAC 1 cut(s) 2778
XmnI GAANNNNTTC 2 cut(s) 270, 2862
XspI CTAG 9 cut(s) 303, 335, 402, 881, 1115, 1650, 2550, 2867, 3205
ZraI GACGTC 1 cut(s) 1922
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.