Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 1017 |
| Acc36I | ACCTGC | 1 cut(s) | 172 |
| AccI | GTMKAC | 1 cut(s) | 1440 |
| AciI | CCGC | 5 cut(s) | 146, 230, 315, 468, 1169 |
| AclWI | GGATC | 2 cut(s) | 449, 698 |
| AcoI | YGGCCR | 1 cut(s) | 243 |
| AcsI | RAATTY | 3 cut(s) | 160, 780, 1281 |
| AcuI | CTGAAG | 2 cut(s) | 795, 882 |
| AfaI | GTAC | 2 cut(s) | 739, 858 |
| AgsI | TTSAA | 9 cut(s) | 32, 47, 121, 421, 440, 762, 893, 984, 1196 |
| AhlI | ACTAGT | 1 cut(s) | 601 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1318, 1350 |
| AluBI | AGCT | 8 cut(s) | 220, 276, 473, 662, 815, 889, 1265, 1427 |
| AluI | AGCT | 8 cut(s) | 220, 276, 473, 662, 815, 889, 1265, 1427 |
| Alw21I | GWGCWC | 1 cut(s) | 609 |
| Alw26I | GTCTC | 1 cut(s) | 1380 |
| Alw44I | GTGCAC | 1 cut(s) | 605 |
| AlwI | GGATC | 2 cut(s) | 449, 698 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 476 |
| AoxI | GGCC | 2 cut(s) | 143, 243 |
| ApaLI | GTGCAC | 1 cut(s) | 605 |
| ApeKI | GCWGC | 7 cut(s) | 294, 470, 473, 662, 965, 1070, 1073 |
| ApoI | RAATTY | 3 cut(s) | 160, 780, 1281 |
| AspLEI | GCGC | 3 cut(s) | 299, 700, 1357 |
| AspS9I | GGNCC | 1 cut(s) | 143 |
| AsuC2I | CCSGG | 1 cut(s) | 1000 |
| AsuHPI | GGTGA | 1 cut(s) | 27 |
| AsuII | TTCGAA | 1 cut(s) | 846 |
| AsuNHI | GCTAGC | 1 cut(s) | 388 |
| BaeGI | GKGCMC | 1 cut(s) | 609 |
| BalI | TGGCCA | 1 cut(s) | 245 |
| Bbv12I | GWGCWC | 1 cut(s) | 609 |
| BbvI | GCAGC | 7 cut(s) | 306, 460, 482, 649, 977, 1057, 1060 |
| BccI | CCATC | 3 cut(s) | 686, 847, 1181 |
| BciVI | GTATCC | 3 cut(s) | 348, 1100, 1183 |
| BclI | TGATCA | 1 cut(s) | 822 |
| BcnI | CCSGG | 1 cut(s) | 1000 |
| BcoDI | GTCTC | 1 cut(s) | 1380 |
| BcuI | ACTAGT | 1 cut(s) | 601 |
| BfaI | CTAG | 5 cut(s) | 389, 540, 602, 830, 1022 |
| BfuAI | ACCTGC | 1 cut(s) | 172 |
| BfuI | GTATCC | 3 cut(s) | 348, 1100, 1183 |
| BisI | GCNGC | 9 cut(s) | 146, 295, 468, 471, 474, 663, 966, 1071, 1074 |
| BlsI | GCNGC | 9 cut(s) | 147, 296, 469, 472, 475, 664, 967, 1072, 1075 |
| Bme1390I | CCNGG | 1 cut(s) | 1000 |
| BmgT120I | GGNCC | 1 cut(s) | 143 |
| BmiI | GGNNCC | 2 cut(s) | 570, 997 |
| BmrFI | CCNGG | 1 cut(s) | 1000 |
| BmrI | ACTGGG | 3 cut(s) | 1001, 1097, 1414 |
| BmsI | GCATC | 2 cut(s) | 266, 1453 |
| BmtI | GCTAGC | 1 cut(s) | 392 |
| BmuI | ACTGGG | 3 cut(s) | 1001, 1097, 1414 |
| Bpu14I | TTCGAA | 1 cut(s) | 846 |
| BpuMI | CCSGG | 1 cut(s) | 1000 |
| BsaJI | CCNNGG | 3 cut(s) | 444, 556, 646 |
| BsaWI | WCCGGW | 1 cut(s) | 866 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 651, 681, 911, 941 |
| Bse118I | RCCGGY | 1 cut(s) | 959 |
| Bse1I | ACTGG | 6 cut(s) | 271, 592, 757, 996, 1092, 1409 |
| Bse3DI | GCAATG | 3 cut(s) | 378, 568, 678 |
| BseDI | CCNNGG | 3 cut(s) | 444, 556, 646 |
| BseGI | GGATG | 1 cut(s) | 1135 |
| BseMI | GCAATG | 3 cut(s) | 378, 568, 678 |
| BseNI | ACTGG | 6 cut(s) | 271, 592, 757, 996, 1092, 1409 |
| BseRI | GAGGAG | 1 cut(s) | 672 |
| BseSI | GKGCMC | 1 cut(s) | 609 |
| BseXI | GCAGC | 7 cut(s) | 306, 460, 482, 649, 977, 1057, 1060 |
| BsgI | GTGCAG | 1 cut(s) | 984 |
| BshFI | GGCC | 2 cut(s) | 145, 245 |
| BsiHKAI | GWGCWC | 1 cut(s) | 609 |
| BsiSI | CCGG | 3 cut(s) | 867, 960, 999 |
| BslFI | GGGAC | 2 cut(s) | 99, 791 |
| BsmAI | GTCTC | 1 cut(s) | 1380 |
| BsmFI | GGGAC | 2 cut(s) | 99, 791 |
| BsmI | GAATGC | 2 cut(s) | 396, 953 |
| BsnI | GGCC | 2 cut(s) | 145, 245 |
| Bsp119I | TTCGAA | 1 cut(s) | 846 |
| Bsp1286I | GDGCHC | 1 cut(s) | 609 |
| Bsp1407I | TGTACA | 1 cut(s) | 737 |
| Bsp143I | GATC | 3 cut(s) | 454, 690, 822 |
| BspACI | CCGC | 5 cut(s) | 146, 230, 315, 468, 1169 |
| BspANI | GGCC | 2 cut(s) | 145, 245 |
| BspLI | GGNNCC | 2 cut(s) | 570, 997 |
| BspMI | ACCTGC | 1 cut(s) | 172 |
| BspOI | GCTAGC | 1 cut(s) | 392 |
| BspPI | GGATC | 2 cut(s) | 449, 698 |
| BspT104I | TTCGAA | 1 cut(s) | 846 |
| BsrDI | GCAATG | 3 cut(s) | 378, 568, 678 |
| BsrFI | RCCGGY | 1 cut(s) | 959 |
| BsrGI | TGTACA | 1 cut(s) | 737 |
| BsrI | ACTGG | 6 cut(s) | 271, 592, 757, 996, 1092, 1409 |
| BssAI | RCCGGY | 1 cut(s) | 959 |
| BssECI | CCNNGG | 3 cut(s) | 444, 556, 646 |
| BssMI | GATC | 3 cut(s) | 454, 690, 822 |
| BssNAI | GTATAC | 1 cut(s) | 1441 |
| BssT1I | CCWWGG | 3 cut(s) | 444, 556, 646 |
| Bst1107I | GTATAC | 1 cut(s) | 1441 |
| Bst4CI | ACNGT | 3 cut(s) | 339, 1112, 1374 |
| BstAUI | TGTACA | 1 cut(s) | 737 |
| BstBI | TTCGAA | 1 cut(s) | 846 |
| BstC8I | GCNNGC | 7 cut(s) | 179, 317, 390, 394, 427, 970, 1368 |
| BstF5I | GGATG | 1 cut(s) | 1135 |
| BstHHI | GCGC | 3 cut(s) | 299, 700, 1357 |
| BstKTI | GATC | 3 cut(s) | 457, 693, 825 |
| BstMAI | GTCTC | 1 cut(s) | 1380 |
| BstMBI | GATC | 3 cut(s) | 454, 690, 822 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 242, 473, 668, 965, 1022, 1363 |
| BstNSI | RCATGY | 2 cut(s) | 181, 927 |
| BstSCI | CCNGG | 1 cut(s) | 998 |
| BstSLI | GKGCMC | 1 cut(s) | 609 |
| BstV1I | GCAGC | 7 cut(s) | 306, 460, 482, 649, 977, 1057, 1060 |
| BstX2I | RGATCY | 1 cut(s) | 454 |
| BstYI | RGATCY | 1 cut(s) | 454 |
| BstZ17I | GTATAC | 1 cut(s) | 1441 |
| BsuI | GTATCC | 3 cut(s) | 348, 1100, 1183 |
| BsuRI | GGCC | 2 cut(s) | 145, 245 |
| BtsCI | GGATG | 1 cut(s) | 1135 |
| BtsI | GCAGTG | 1 cut(s) | 1126 |
| BtsIMutI | CAGTG | 4 cut(s) | 585, 906, 1126, 1370 |
| BveI | ACCTGC | 1 cut(s) | 172 |
| Cac8I | GCNNGC | 7 cut(s) | 179, 317, 390, 394, 427, 970, 1368 |
| CaiI | CAGNNNCTG | 1 cut(s) | 476 |
| CfoI | GCGC | 3 cut(s) | 299, 700, 1357 |
| Cfr10I | RCCGGY | 1 cut(s) | 959 |
| Cfr13I | GGNCC | 1 cut(s) | 143 |
| Csp6I | GTAC | 2 cut(s) | 738, 857 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 235, 270 |
| CviAII | CATG | 7 cut(s) | 178, 196, 747, 776, 924, 931, 940 |
| CviQI | GTAC | 2 cut(s) | 738, 857 |
| DpnI | GATC | 3 cut(s) | 456, 692, 824 |
| DpnII | GATC | 3 cut(s) | 454, 690, 822 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 1017 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 1017 |
| EaeI | YGGCCR | 1 cut(s) | 243 |
| Eco130I | CCWWGG | 3 cut(s) | 444, 556, 646 |
| Eco57I | CTGAAG | 2 cut(s) | 795, 882 |
| EcoRI | GAATTC | 2 cut(s) | 780, 1281 |
| EcoT14I | CCWWGG | 3 cut(s) | 444, 556, 646 |
| EcoT22I | ATGCAT | 2 cut(s) | 1046, 1468 |
| ErhI | CCWWGG | 3 cut(s) | 444, 556, 646 |
| FaeI | CATG | 7 cut(s) | 181, 199, 750, 779, 927, 934, 943 |
| FaqI | GGGAC | 2 cut(s) | 99, 791 |
| FatI | CATG | 7 cut(s) | 177, 195, 746, 775, 923, 930, 939 |
| FauI | CCCGC | 2 cut(s) | 223, 308 |
| FauNDI | CATATG | 3 cut(s) | 325, 345, 1249 |
| FbaI | TGATCA | 1 cut(s) | 822 |
| FblI | GTMKAC | 1 cut(s) | 1440 |
| Fnu4HI | GCNGC | 9 cut(s) | 146, 295, 468, 471, 474, 663, 966, 1071, 1074 |
| FokI | GGATG | 1 cut(s) | 1142 |
| Fsp4HI | GCNGC | 9 cut(s) | 146, 295, 468, 471, 474, 663, 966, 1071, 1074 |
| FspBI | CTAG | 5 cut(s) | 389, 540, 602, 830, 1022 |
| GlaI | GCGC | 3 cut(s) | 298, 699, 1356 |
| GluI | GCNGC | 9 cut(s) | 146, 295, 468, 471, 474, 663, 966, 1071, 1074 |
| HaeIII | GGCC | 2 cut(s) | 145, 245 |
| HapII | CCGG | 3 cut(s) | 867, 960, 999 |
| HhaI | GCGC | 3 cut(s) | 299, 700, 1357 |
| Hin1II | CATG | 7 cut(s) | 181, 199, 750, 779, 927, 934, 943 |
| Hin6I | GCGC | 3 cut(s) | 297, 698, 1355 |
| HinP1I | GCGC | 3 cut(s) | 297, 698, 1355 |
| HindIII | AAGCTT | 2 cut(s) | 274, 1425 |
| HinfI | GANTC | 5 cut(s) | 32, 43, 543, 943, 1344 |
| HpaII | CCGG | 3 cut(s) | 867, 960, 999 |
| HphI | GGTGA | 1 cut(s) | 27 |
| Hpy166II | GTNNAC | 4 cut(s) | 607, 740, 1229, 1441 |
| Hpy188I | TCNGA | 2 cut(s) | 1432, 1462 |
| Hpy188III | TCNNGA | 6 cut(s) | 29, 94, 452, 458, 496, 540 |
| Hpy8I | GTNNAC | 4 cut(s) | 607, 740, 1229, 1441 |
| HpyAV | CCTTC | 3 cut(s) | 819, 1052, 1405 |
| HpyCH4III | ACNGT | 3 cut(s) | 339, 1112, 1374 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 242, 473, 668, 965, 1022, 1363 |
| Hsp92II | CATG | 7 cut(s) | 181, 199, 750, 779, 927, 934, 943 |
| HspAI | GCGC | 3 cut(s) | 297, 698, 1355 |
| Ksp22I | TGATCA | 1 cut(s) | 822 |
| Kzo9I | GATC | 3 cut(s) | 454, 690, 822 |
| LmnI | GCTCC | 6 cut(s) | 217, 329, 659, 932, 995, 1262 |
| Lsp1109I | GCAGC | 7 cut(s) | 306, 460, 482, 649, 977, 1057, 1060 |
| LweI | GCATC | 2 cut(s) | 266, 1453 |
| MaeI | CTAG | 5 cut(s) | 389, 540, 602, 830, 1022 |
| MaeIII | GTNAC | 3 cut(s) | 262, 1051, 1112 |
| MalI | GATC | 3 cut(s) | 456, 692, 824 |
| MboI | GATC | 3 cut(s) | 454, 690, 822 |
| MboII | GAAGA | 7 cut(s) | 23, 133, 374, 428, 866, 1249, 1432 |
| MflI | RGATCY | 1 cut(s) | 454 |
| MhlI | GDGCHC | 1 cut(s) | 609 |
| MlsI | TGGCCA | 1 cut(s) | 245 |
| MluNI | TGGCCA | 1 cut(s) | 245 |
| MmeI | TCCRAC | 2 cut(s) | 550, 1126 |
| MnlI | CCTC | 9 cut(s) | 106, 360, 411, 650, 742, 760, 957, 1214, 1316 |
| Mox20I | TGGCCA | 1 cut(s) | 245 |
| Mph1103I | ATGCAT | 2 cut(s) | 1046, 1468 |
| MscI | TGGCCA | 1 cut(s) | 245 |
| MseI | TTAA | 3 cut(s) | 272, 918, 1254 |
| MslI | CAYNNNNRTG | 1 cut(s) | 745 |
| Msp20I | TGGCCA | 1 cut(s) | 245 |
| MspA1I | CMGCKG | 2 cut(s) | 148, 473 |
| MspI | CCGG | 3 cut(s) | 867, 960, 999 |
| MspR9I | CCNGG | 1 cut(s) | 1000 |
| Mva1269I | GAATGC | 2 cut(s) | 396, 953 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 242, 473, 668, 965, 1022, 1363 |
| NciI | CCSGG | 1 cut(s) | 1000 |
| NdeI | CATATG | 3 cut(s) | 325, 345, 1249 |
| NdeII | GATC | 3 cut(s) | 454, 690, 822 |
| NheI | GCTAGC | 1 cut(s) | 388 |
| NlaIII | CATG | 7 cut(s) | 181, 199, 750, 779, 927, 934, 943 |
| NlaIV | GGNNCC | 2 cut(s) | 570, 997 |
| NmuCI | GTSAC | 1 cut(s) | 262 |
| NsiI | ATGCAT | 2 cut(s) | 1046, 1468 |
| NspI | RCATGY | 2 cut(s) | 181, 927 |
| NspV | TTCGAA | 1 cut(s) | 846 |
| PaeI | GCATGC | 1 cut(s) | 181 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1015 |
| PctI | GAATGC | 2 cut(s) | 396, 953 |
| PfeI | GAWTC | 5 cut(s) | 32, 43, 543, 943, 1344 |
| PkrI | GCNGC | 9 cut(s) | 147, 296, 469, 472, 475, 664, 967, 1072, 1075 |
| PspN4I | GGNNCC | 2 cut(s) | 570, 997 |
| PspPI | GGNCC | 1 cut(s) | 143 |
| PstNI | CAGNNNCTG | 1 cut(s) | 476 |
| PsuI | RGATCY | 1 cut(s) | 454 |
| PvuII | CAGCTG | 1 cut(s) | 473 |
| RsaI | GTAC | 2 cut(s) | 739, 858 |
| RsaNI | GTAC | 2 cut(s) | 738, 857 |
| RseI | CAYNNNNRTG | 1 cut(s) | 745 |
| SaqAI | TTAA | 3 cut(s) | 272, 918, 1254 |
| SatI | GCNGC | 9 cut(s) | 146, 295, 468, 471, 474, 663, 966, 1071, 1074 |
| Sau3AI | GATC | 3 cut(s) | 454, 690, 822 |
| Sau96I | GGNCC | 1 cut(s) | 143 |
| ScrFI | CCNGG | 1 cut(s) | 1000 |
| SduI | GDGCHC | 1 cut(s) | 609 |
| SfaNI | GCATC | 2 cut(s) | 266, 1453 |
| SfuI | TTCGAA | 1 cut(s) | 846 |
| SgrAI | CRCCGGYG | 1 cut(s) | 959 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 745 |
| SpeI | ACTAGT | 1 cut(s) | 601 |
| SphI | GCATGC | 1 cut(s) | 181 |
| SsiI | CCGC | 5 cut(s) | 146, 230, 315, 468, 1169 |
| SspI | AATATT | 1 cut(s) | 1296 |
| SspMI | CTAG | 5 cut(s) | 389, 540, 602, 830, 1022 |
| StyD4I | CCNGG | 1 cut(s) | 998 |
| StyI | CCWWGG | 3 cut(s) | 444, 556, 646 |
| TaaI | ACNGT | 3 cut(s) | 339, 1112, 1374 |
| TaqI | TCGA | 3 cut(s) | 111, 846, 1093 |
| TatI | WGTACW | 1 cut(s) | 737 |
| TauI | GCSGC | 2 cut(s) | 148, 470 |
| TfiI | GAWTC | 5 cut(s) | 32, 43, 543, 943, 1344 |
| Tru1I | TTAA | 3 cut(s) | 272, 918, 1254 |
| Tru9I | TTAA | 3 cut(s) | 272, 918, 1254 |
| TscAI | CASTG | 4 cut(s) | 592, 906, 1126, 1377 |
| TseFI | GTSAC | 1 cut(s) | 262 |
| TseI | GCWGC | 7 cut(s) | 294, 470, 473, 662, 965, 1070, 1073 |
| Tsp45I | GTSAC | 1 cut(s) | 262 |
| TspDTI | ATGAA | 3 cut(s) | 240, 764, 781 |
| TspRI | CASTG | 4 cut(s) | 592, 906, 1126, 1377 |
| VneI | GTGCAC | 1 cut(s) | 605 |
| XapI | RAATTY | 3 cut(s) | 160, 780, 1281 |
| XbaI | TCTAGA | 1 cut(s) | 539 |
| XceI | RCATGY | 2 cut(s) | 181, 927 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1184 |
| XmiI | GTMKAC | 1 cut(s) | 1440 |
| XspI | CTAG | 5 cut(s) | 389, 540, 602, 830, 1022 |
| Zsp2I | ATGCAT | 2 cut(s) | 1046, 1468 |