Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 2 cut(s) | 352, 630 |
| AccII | CGCG | 2 cut(s) | 1015, 1454 |
| AciI | CCGC | 5 cut(s) | 143, 312, 835, 1004, 1454 |
| AclWI | GGATC | 3 cut(s) | 446, 655, 695 |
| AcoI | YGGCCR | 1 cut(s) | 140 |
| AcsI | RAATTY | 2 cut(s) | 157, 777 |
| AcuI | CTGAAG | 4 cut(s) | 240, 419, 792, 1074 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 94, 1315 |
| AflIII | ACRYGT | 1 cut(s) | 920 |
| AgsI | TTSAA | 6 cut(s) | 32, 71, 237, 845, 890, 981 |
| AjnI | CCWGG | 2 cut(s) | 945, 1347 |
| AluBI | AGCT | 6 cut(s) | 127, 217, 273, 812, 825, 1473 |
| AluI | AGCT | 6 cut(s) | 127, 217, 273, 812, 825, 1473 |
| Alw21I | GWGCWC | 1 cut(s) | 1440 |
| Alw44I | GTGCAC | 1 cut(s) | 1436 |
| AlwI | GGATC | 3 cut(s) | 446, 655, 695 |
| AlwNI | CAGNNNCTG | 3 cut(s) | 263, 590, 1369 |
| AoxI | GGCC | 3 cut(s) | 140, 765, 1187 |
| ApaLI | GTGCAC | 1 cut(s) | 1436 |
| ApeKI | GCWGC | 7 cut(s) | 124, 470, 659, 1022, 1070, 1142, 1228 |
| ApoI | RAATTY | 2 cut(s) | 157, 777 |
| AspLEI | GCGC | 4 cut(s) | 296, 1015, 1354, 1454 |
| AspS9I | GGNCC | 2 cut(s) | 853, 1187 |
| AsuHPI | GGTGA | 2 cut(s) | 670, 1496 |
| AvaII | GGWCC | 1 cut(s) | 853 |
| BaeGI | GKGCMC | 1 cut(s) | 1440 |
| Bbv12I | GWGCWC | 1 cut(s) | 1440 |
| BbvI | GCAGC | 7 cut(s) | 136, 457, 646, 1034, 1057, 1129, 1240 |
| BccI | CCATC | 2 cut(s) | 683, 1178 |
| BciT130I | CCWGG | 2 cut(s) | 947, 1349 |
| BclI | TGATCA | 1 cut(s) | 1489 |
| BfaI | CTAG | 4 cut(s) | 537, 651, 1010, 1019 |
| BfmI | CTRYAG | 2 cut(s) | 471, 1143 |
| BfoI | RGCGCY | 2 cut(s) | 297, 1355 |
| BisI | GCNGC | 8 cut(s) | 125, 143, 471, 660, 1023, 1071, 1143, 1229 |
| BlsI | GCNGC | 8 cut(s) | 126, 144, 472, 661, 1024, 1072, 1144, 1230 |
| Bme1390I | CCNGG | 2 cut(s) | 947, 1349 |
| Bme18I | GGWCC | 1 cut(s) | 853 |
| BmgT120I | GGNCC | 2 cut(s) | 853, 1187 |
| BmrFI | CCNGG | 2 cut(s) | 947, 1349 |
| BmrI | ACTGGG | 1 cut(s) | 290 |
| BmsI | GCATC | 4 cut(s) | 333, 1433, 1450, 1472 |
| BmuI | ACTGGG | 1 cut(s) | 290 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 449, 481 |
| BpmI | CTGGAG | 1 cut(s) | 1308 |
| Bpu10I | CCTNAGC | 1 cut(s) | 442 |
| BpuEI | CTTGAG | 1 cut(s) | 39 |
| BsaJI | CCNNGG | 4 cut(s) | 297, 643, 772, 945 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 94, 1315 |
| Bse1I | ACTGG | 4 cut(s) | 268, 285, 754, 1291 |
| Bse3DI | GCAATG | 3 cut(s) | 375, 565, 933 |
| BseBI | CCWGG | 2 cut(s) | 947, 1349 |
| BseDI | CCNNGG | 4 cut(s) | 297, 643, 772, 945 |
| BseGI | GGATG | 2 cut(s) | 103, 348 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 94, 1315 |
| BseMI | GCAATG | 3 cut(s) | 375, 565, 933 |
| BseMII | CTCAG | 1 cut(s) | 1205 |
| BseNI | ACTGG | 4 cut(s) | 268, 285, 754, 1291 |
| BseSI | GKGCMC | 1 cut(s) | 1440 |
| BseXI | GCAGC | 7 cut(s) | 136, 457, 646, 1034, 1057, 1129, 1240 |
| BseYI | CCCAGC | 1 cut(s) | 995 |
| BsgI | GTGCAG | 2 cut(s) | 587, 1382 |
| Bsh1236I | CGCG | 2 cut(s) | 1015, 1454 |
| BshFI | GGCC | 3 cut(s) | 142, 767, 1189 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1440 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 94, 1315 |
| BsmI | GAATGC | 1 cut(s) | 1278 |
| BsnI | GGCC | 3 cut(s) | 142, 767, 1189 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1440 |
| Bsp143I | GATC | 5 cut(s) | 25, 451, 647, 687, 1489 |
| Bsp19I | CCATGG | 1 cut(s) | 772 |
| BspACI | CCGC | 5 cut(s) | 143, 312, 835, 1004, 1454 |
| BspANI | GGCC | 3 cut(s) | 142, 767, 1189 |
| BspCNI | CTCAG | 1 cut(s) | 1204 |
| BspFNI | CGCG | 2 cut(s) | 1015, 1454 |
| BspHI | TCATGA | 1 cut(s) | 1077 |
| BspMAI | CTGCAG | 2 cut(s) | 475, 1147 |
| BspPI | GGATC | 3 cut(s) | 446, 655, 695 |
| BsrDI | GCAATG | 3 cut(s) | 375, 565, 933 |
| BsrI | ACTGG | 4 cut(s) | 268, 285, 754, 1291 |
| BssECI | CCNNGG | 4 cut(s) | 297, 643, 772, 945 |
| BssMI | GATC | 5 cut(s) | 25, 451, 647, 687, 1489 |
| BssNAI | GTATAC | 2 cut(s) | 353, 631 |
| BssT1I | CCWWGG | 3 cut(s) | 297, 643, 772 |
| Bst1107I | GTATAC | 2 cut(s) | 353, 631 |
| Bst2UI | CCWGG | 2 cut(s) | 947, 1349 |
| Bst4CI | ACNGT | 5 cut(s) | 39, 336, 798, 1109, 1371 |
| Bst6I | CTCTTC | 1 cut(s) | 1087 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1031 |
| BstC8I | GCNNGC | 4 cut(s) | 225, 424, 580, 1456 |
| BstDEI | CTNAG | 3 cut(s) | 442, 826, 1191 |
| BstDSI | CCRYGG | 1 cut(s) | 772 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 92 |
| BstF5I | GGATG | 2 cut(s) | 103, 348 |
| BstFNI | CGCG | 2 cut(s) | 1015, 1454 |
| BstH2I | RGCGCY | 2 cut(s) | 297, 1355 |
| BstHHI | GCGC | 4 cut(s) | 296, 1015, 1354, 1454 |
| BstKTI | GATC | 5 cut(s) | 28, 454, 650, 690, 1492 |
| BstMBI | GATC | 5 cut(s) | 25, 451, 647, 687, 1489 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 239, 665, 1012, 1019, 1031, 1360, 1460 |
| BstNI | CCWGG | 2 cut(s) | 947, 1349 |
| BstNSI | RCATGY | 3 cut(s) | 65, 924, 1029 |
| BstSCI | CCNGG | 2 cut(s) | 945, 1347 |
| BstSFI | CTRYAG | 2 cut(s) | 471, 1143 |
| BstSLI | GKGCMC | 1 cut(s) | 1440 |
| BstUI | CGCG | 2 cut(s) | 1015, 1454 |
| BstV1I | GCAGC | 7 cut(s) | 136, 457, 646, 1034, 1057, 1129, 1240 |
| BstX2I | RGATCY | 2 cut(s) | 451, 647 |
| BstYI | RGATCY | 2 cut(s) | 451, 647 |
| BstZ17I | GTATAC | 2 cut(s) | 353, 631 |
| BsuRI | GGCC | 3 cut(s) | 142, 767, 1189 |
| BtgI | CCRYGG | 1 cut(s) | 772 |
| BtgZI | GCGATG | 2 cut(s) | 1449, 1473 |
| BtsCI | GGATG | 2 cut(s) | 103, 348 |
| BtsI | GCAGTG | 1 cut(s) | 1123 |
| BtsIMutI | CAGTG | 5 cut(s) | 35, 261, 443, 1123, 1367 |
| Cac8I | GCNNGC | 4 cut(s) | 225, 424, 580, 1456 |
| CaiI | CAGNNNCTG | 3 cut(s) | 263, 590, 1369 |
| CciI | TCATGA | 1 cut(s) | 1077 |
| CfoI | GCGC | 4 cut(s) | 296, 1015, 1354, 1454 |
| Cfr13I | GGNCC | 2 cut(s) | 853, 1187 |
| CseI | GACGC | 1 cut(s) | 1004 |
| DdeI | CTNAG | 3 cut(s) | 442, 826, 1191 |
| DpnI | GATC | 5 cut(s) | 27, 453, 649, 689, 1491 |
| DpnII | GATC | 5 cut(s) | 25, 451, 647, 687, 1489 |
| EaeI | YGGCCR | 1 cut(s) | 140 |
| Eam1104I | CTCTTC | 1 cut(s) | 1087 |
| EarI | CTCTTC | 1 cut(s) | 1087 |
| Eco130I | CCWWGG | 3 cut(s) | 297, 643, 772 |
| Eco147I | AGGCCT | 1 cut(s) | 767 |
| Eco32I | GATATC | 1 cut(s) | 462 |
| Eco47I | GGWCC | 1 cut(s) | 853 |
| Eco57I | CTGAAG | 4 cut(s) | 240, 419, 792, 1074 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 92 |
| EcoRI | GAATTC | 1 cut(s) | 777 |
| EcoRII | CCWGG | 2 cut(s) | 945, 1347 |
| EcoRV | GATATC | 1 cut(s) | 462 |
| EcoT14I | CCWWGG | 3 cut(s) | 297, 643, 772 |
| EcoT22I | ATGCAT | 2 cut(s) | 697, 1465 |
| ErhI | CCWWGG | 3 cut(s) | 297, 643, 772 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 752, 784 |
| FauI | CCCGC | 1 cut(s) | 1447 |
| FauNDI | CATATG | 3 cut(s) | 357, 1246, 1494 |
| FbaI | TGATCA | 1 cut(s) | 1489 |
| FblI | GTMKAC | 2 cut(s) | 352, 630 |
| Fnu4HI | GCNGC | 8 cut(s) | 125, 143, 471, 660, 1023, 1071, 1143, 1229 |
| FokI | GGATG | 2 cut(s) | 110, 355 |
| Fsp4HI | GCNGC | 8 cut(s) | 125, 143, 471, 660, 1023, 1071, 1143, 1229 |
| FspBI | CTAG | 4 cut(s) | 537, 651, 1010, 1019 |
| GlaI | GCGC | 4 cut(s) | 295, 1014, 1353, 1453 |
| GluI | GCNGC | 8 cut(s) | 125, 143, 471, 660, 1023, 1071, 1143, 1229 |
| GsaI | CCCAGC | 1 cut(s) | 999 |
| GsuI | CTGGAG | 1 cut(s) | 1308 |
| HaeII | RGCGCY | 2 cut(s) | 297, 1355 |
| HaeIII | GGCC | 3 cut(s) | 142, 767, 1189 |
| HgaI | GACGC | 1 cut(s) | 1004 |
| HhaI | GCGC | 4 cut(s) | 296, 1015, 1354, 1454 |
| Hin6I | GCGC | 4 cut(s) | 294, 1013, 1352, 1452 |
| HinP1I | GCGC | 4 cut(s) | 294, 1013, 1352, 1452 |
| HinfI | GANTC | 9 cut(s) | 32, 43, 71, 84, 540, 565, 753, 877, 940 |
| HphI | GGTGA | 2 cut(s) | 670, 1496 |
| Hpy166II | GTNNAC | 4 cut(s) | 353, 631, 1135, 1438 |
| Hpy188I | TCNGA | 5 cut(s) | 342, 570, 719, 876, 1429 |
| Hpy188III | TCNNGA | 8 cut(s) | 29, 56, 75, 88, 449, 455, 651, 1078 |
| Hpy8I | GTNNAC | 4 cut(s) | 353, 631, 1135, 1438 |
| HpyAV | CCTTC | 4 cut(s) | 778, 816, 1049, 1318 |
| HpyCH4III | ACNGT | 5 cut(s) | 39, 336, 798, 1109, 1371 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 239, 665, 1012, 1019, 1031, 1360, 1460 |
| HpyF3I | CTNAG | 3 cut(s) | 442, 826, 1191 |
| HspAI | GCGC | 4 cut(s) | 294, 1013, 1352, 1452 |
| Ksp22I | TGATCA | 1 cut(s) | 1489 |
| Kzo9I | GATC | 5 cut(s) | 25, 451, 647, 687, 1489 |
| LmnI | GCTCC | 1 cut(s) | 326 |
| Lsp1109I | GCAGC | 7 cut(s) | 136, 457, 646, 1034, 1057, 1129, 1240 |
| LweI | GCATC | 4 cut(s) | 333, 1433, 1450, 1472 |
| MaeI | CTAG | 4 cut(s) | 537, 651, 1010, 1019 |
| MaeIII | GTNAC | 3 cut(s) | 259, 1109, 1365 |
| MalI | GATC | 5 cut(s) | 27, 453, 649, 689, 1491 |
| MboI | GATC | 5 cut(s) | 25, 451, 647, 687, 1489 |
| MboII | GAAGA | 7 cut(s) | 23, 26, 130, 425, 1104, 1246, 1429 |
| MfeI | CAATTG | 1 cut(s) | 986 |
| MflI | RGATCY | 2 cut(s) | 451, 647 |
| MhlI | GDGCHC | 1 cut(s) | 1440 |
| MluCI | AATT | 7 cut(s) | 49, 157, 405, 777, 986, 1341, 1380 |
| Mph1103I | ATGCAT | 2 cut(s) | 697, 1465 |
| MseI | TTAA | 4 cut(s) | 134, 815, 915, 1251 |
| MspA1I | CMGCKG | 1 cut(s) | 127 |
| MspR9I | CCNGG | 2 cut(s) | 947, 1349 |
| MunI | CAATTG | 1 cut(s) | 986 |
| Mva1269I | GAATGC | 1 cut(s) | 1278 |
| MvaI | CCWGG | 2 cut(s) | 947, 1349 |
| MvnI | CGCG | 2 cut(s) | 1015, 1454 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 239, 665, 1012, 1019, 1031, 1360, 1460 |
| NcoI | CCATGG | 1 cut(s) | 772 |
| NdeI | CATATG | 3 cut(s) | 357, 1246, 1494 |
| NdeII | GATC | 5 cut(s) | 25, 451, 647, 687, 1489 |
| NmuCI | GTSAC | 2 cut(s) | 259, 1365 |
| NsiI | ATGCAT | 2 cut(s) | 697, 1465 |
| NspI | RCATGY | 3 cut(s) | 65, 924, 1029 |
| PagI | TCATGA | 1 cut(s) | 1077 |
| PceI | AGGCCT | 1 cut(s) | 767 |
| PciI | ACATGT | 1 cut(s) | 920 |
| PctI | GAATGC | 1 cut(s) | 1278 |
| PfeI | GAWTC | 9 cut(s) | 32, 43, 71, 84, 540, 565, 753, 877, 940 |
| PkrI | GCNGC | 8 cut(s) | 126, 144, 472, 661, 1024, 1072, 1144, 1230 |
| PscI | ACATGT | 1 cut(s) | 920 |
| Psp6I | CCWGG | 2 cut(s) | 945, 1347 |
| PspFI | CCCAGC | 1 cut(s) | 995 |
| PspGI | CCWGG | 2 cut(s) | 945, 1347 |
| PspPI | GGNCC | 2 cut(s) | 853, 1187 |
| PstI | CTGCAG | 2 cut(s) | 475, 1147 |
| PstNI | CAGNNNCTG | 3 cut(s) | 263, 590, 1369 |
| PsuI | RGATCY | 2 cut(s) | 451, 647 |
| PvuII | CAGCTG | 1 cut(s) | 127 |
| SaqAI | TTAA | 4 cut(s) | 134, 815, 915, 1251 |
| SatI | GCNGC | 8 cut(s) | 125, 143, 471, 660, 1023, 1071, 1143, 1229 |
| Sau3AI | GATC | 5 cut(s) | 25, 451, 647, 687, 1489 |
| Sau96I | GGNCC | 2 cut(s) | 853, 1187 |
| ScrFI | CCNGG | 2 cut(s) | 947, 1349 |
| SduI | GDGCHC | 1 cut(s) | 1440 |
| SfaNI | GCATC | 4 cut(s) | 333, 1433, 1450, 1472 |
| SfcI | CTRYAG | 2 cut(s) | 471, 1143 |
| SinI | GGWCC | 1 cut(s) | 853 |
| SmlI | CTYRAG | 1 cut(s) | 54 |
| SmoI | CTYRAG | 1 cut(s) | 54 |
| Sse9I | AATT | 7 cut(s) | 49, 157, 405, 777, 986, 1341, 1380 |
| SseBI | AGGCCT | 1 cut(s) | 767 |
| SsiI | CCGC | 5 cut(s) | 143, 312, 835, 1004, 1454 |
| SspI | AATATT | 2 cut(s) | 430, 1152 |
| SspMI | CTAG | 4 cut(s) | 537, 651, 1010, 1019 |
| StuI | AGGCCT | 1 cut(s) | 767 |
| StyD4I | CCNGG | 2 cut(s) | 945, 1347 |
| StyI | CCWWGG | 3 cut(s) | 297, 643, 772 |
| TaaI | ACNGT | 5 cut(s) | 39, 336, 798, 1109, 1371 |
| TaqI | TCGA | 2 cut(s) | 74, 1090 |
| TaqII | GACCGA | 1 cut(s) | 191 |
| TasI | AATT | 7 cut(s) | 49, 157, 405, 777, 986, 1341, 1380 |
| TauI | GCSGC | 1 cut(s) | 145 |
| TfiI | GAWTC | 9 cut(s) | 32, 43, 71, 84, 540, 565, 753, 877, 940 |
| Tru1I | TTAA | 4 cut(s) | 134, 815, 915, 1251 |
| Tru9I | TTAA | 4 cut(s) | 134, 815, 915, 1251 |
| TscAI | CASTG | 5 cut(s) | 42, 268, 443, 1123, 1374 |
| TseFI | GTSAC | 2 cut(s) | 259, 1365 |
| TseI | GCWGC | 7 cut(s) | 124, 470, 659, 1022, 1070, 1142, 1228 |
| Tsp45I | GTSAC | 2 cut(s) | 259, 1365 |
| TspDTI | ATGAA | 1 cut(s) | 237 |
| TspGWI | ACGGA | 1 cut(s) | 1408 |
| TspRI | CASTG | 5 cut(s) | 42, 268, 443, 1123, 1374 |
| VneI | GTGCAC | 1 cut(s) | 1436 |
| VpaK11BI | GGWCC | 1 cut(s) | 853 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 92 |
| XapI | RAATTY | 2 cut(s) | 157, 777 |
| XbaI | TCTAGA | 1 cut(s) | 650 |
| XceI | RCATGY | 3 cut(s) | 65, 924, 1029 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 992, 1181 |
| XmiI | GTMKAC | 2 cut(s) | 352, 630 |
| XspI | CTAG | 4 cut(s) | 537, 651, 1010, 1019 |
| Zsp2I | ATGCAT | 2 cut(s) | 697, 1465 |