Rroxscaffold_3G00223300

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000521
Physical Location & Seq
Reverse (-)
6374246 .. 6375167
922 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00223300.1

Sequence Viewer

Length: 816 bp
ATGGATGTAGAAGAAGAAAGATGTGATCTTGAATCACTGTTGATTCCTAATTCTCAAGAACATGCTATTGAATCGAGAAAGGGATTCCTGAACAAGGATGAGATTTCTGAGGAAGTGAAGAAGCAGCTGTTGTTAATATGGCCGCTTGTGTCATCAAATTTCTTGCTATTTGGTATGCAAGTAATTTCAGTCATGTATGTCGGTTATCTTGGAGAGCTATCGCTCGCTGGTGCTTCAATAGCCCCACTTCATTCGCTTCAGTCACTGCTTGGAATGTGTAGCGCCTTGGACACTTTCAGCGGTCAGTCCTTTGGAGCAAAACAGTATCGGATGCTTGGTATACATATGCAGAGGGCAATGCTTATTCTTTTGCTCGTCAGCGTTCCTCTTGCAATTATATGGGCTAATGCAGGCAATATTCTTCAGTGCTTAGGTCAAGATCCAGAGATATCCTCTGCTGCAGGAGGTTATGCTCGTTTGATGATACCTTGCATTTTTGCTTATGCAATCCTACAATGTCATGCTAGATTCTTGCAAACTCAAAGCAATGTGATTCCAATGATTGCTAGCACAGGAACTGCAACACTACTAAACTTGCTTACCTGTTGGGTTATGGTATACAAGACCAGCCTTGGATATAGAGGTGCTGCTGTGGCAAACGCCATCACCTATTGGATCAATGCATTGTTTTTGTTTATTTATGTCAGAGTTTCTCCCTCTTGCAAGAACACATGGACTGGATTCTCAAAGGAGGCCTTCCACGGAATTCCCACTTTTCTAAAACTATCTATCCCTTCGGCTTTAATGTCCAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

271

Amino Acids

29.84

Weight (kDa)

7.59

Isoelectric Point (pI)

40.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 48 - 204 2.8e-30 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000521)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g09990 FvH4_4g09990 FvH4_4g09990 FvH4_4g09990 FvH4_7g32860 FvH4_7g32860 FvH4_7g32860 FvH4_7g32860
malus_domestica MD11G1148000.v1.1 MD13G1266200.v1.1 MD14G1231800.v1.1
prunus_persica Prupe.1G090100_v2.0.a1 Prupe.1G090100_v2.0.a1 Prupe.1G090100_v2.0.a1 Prupe.1G090100_v2.0.a1 Prupe.1G090100_v2.0.a1 Prupe.1G090300_v2.0.a1 Prupe.1G090300_v2.0.a1 Prupe.1G090300_v2.0.a1 Prupe.1G090300_v2.0.a1 Prupe.1G090300_v2.0.a1
pyrus_communis pycom13g23330
rosa_chinensis RchiOBHm_Chr4g0403401 RchiOBHm_Chr4g0403411 RchiOBHm_Chr4g0403421 RchiOBHm_Chr4g0403461 RchiOBHm_Chr4g0403631 RchiOBHm_Chr7g0223601 RchiOBHm_Chr7g0238161
rosa_laevigata RLG00000000937 RLG00000001957 RLG00000008965 RLG00000008975 RLG00000008976 RLG00000008978
rosa_multiflora Rmu_co8335743.1_g000001 Rmu_sc0005734.1_g000001 Rmu_sc0007727.1_g000011 Rmu_sc0011775.1_g000002 Rmu_sc0011775.1_g000005 Rmu_sc0028062.1_g000002
rosa_roxburghii Rroxscaffold_3G00223300 Rroxscaffold_5G00348050 Rroxscaffold_5G00348060 Rroxscaffold_5G00348120 Rroxscaffold_5G00348240
rosa_rugosa Rorug04G0042300 Rorug04G0042400 Rorug04G0042500 Rorug04G0042600.1 Rorug04G0042700 Rorug04G0042800 Rorug04G0042900 Rorug04G0043000 Rorug04G0043100 Rorug04G0043200 Rorug04G0043300 Rorug04G0043400 Rorug04G0044700 Rorug07G0310600 Rorug07G0310700 Rorug07G0310700
rosa_samantha Rh4AG118700 Rh4AG118800 Rh4AG118900 Rh4AG119000 Rh4BG111600 Rh4BG111800 Rh4BG111900 Rh4BG112900 Rh4CG126600 Rh4CG126900 Rh4CG127800 Rh4DG111300 Rh4DG111400 Rh4DG111500 Rh7AG466500 Rh7BG352200 Rh7BG436900 Rh7CG484200 Rh7DG452600
rosa_wichuraiana Rw4G009630 Rw4G009640 Rw4G009650 Rw4G009710 Rw7G038620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 340, 618
AciI CCGC 2 cut(s) 143, 300
AclWI GGATC 2 cut(s) 434, 683
AcoI YGGCCR 1 cut(s) 140
AcsI RAATTY 2 cut(s) 157, 765
AcuI CTGAAG 2 cut(s) 242, 407
AfiI CCNNNNNNNGG 1 cut(s) 94
AgsI TTSAA 3 cut(s) 32, 71, 237
AjnI CCWGG 1 cut(s) 809
AluBI AGCT 2 cut(s) 127, 217
AluI AGCT 2 cut(s) 127, 217
AlwI GGATC 2 cut(s) 434, 683
AlwNI CAGNNNCTG 2 cut(s) 265, 578
AoxI GGCC 2 cut(s) 140, 753
ApeKI GCWGC 3 cut(s) 124, 458, 647
ApoI RAATTY 2 cut(s) 157, 765
AspLEI GCGC 1 cut(s) 284
AsuHPI GGTGA 1 cut(s) 658
AsuNHI GCTAGC 1 cut(s) 566
BbvI GCAGC 3 cut(s) 136, 445, 634
BccI CCATC 1 cut(s) 671
BciT130I CCWGG 1 cut(s) 811
BfaI CTAG 2 cut(s) 525, 567
BfmI CTRYAG 1 cut(s) 459
BfoI RGCGCY 1 cut(s) 285
BisI GCNGC 4 cut(s) 125, 143, 459, 648
BlsI GCNGC 4 cut(s) 126, 144, 460, 649
Bme1390I CCNGG 1 cut(s) 811
BmrFI CCNGG 1 cut(s) 811
BmsI GCATC 1 cut(s) 321
BmtI GCTAGC 1 cut(s) 570
BplI GAGNNNNNCTC 2 cut(s) 437, 469
Bpu10I CCTNAGC 1 cut(s) 430
BpuEI CTTGAG 1 cut(s) 39
BsaJI CCNNGG 3 cut(s) 285, 631, 760
Bsc4I CCNNNNNNNGG 1 cut(s) 94
Bse1I ACTGG 1 cut(s) 742
Bse3DI GCAATG 2 cut(s) 363, 553
BseBI CCWGG 1 cut(s) 811
BseDI CCNNGG 3 cut(s) 285, 631, 760
BseGI GGATG 3 cut(s) 10, 103, 336
BseLI CCNNNNNNNGG 1 cut(s) 94
BseMI GCAATG 2 cut(s) 363, 553
BseMII CTCAG 1 cut(s) 99
BseNI ACTGG 1 cut(s) 742
BseXI GCAGC 3 cut(s) 136, 445, 634
BshFI GGCC 2 cut(s) 142, 755
BslI CCNNNNNNNGG 1 cut(s) 94
BsnI GGCC 2 cut(s) 142, 755
Bsp143I GATC 3 cut(s) 25, 439, 675
BspACI CCGC 2 cut(s) 143, 300
BspANI GGCC 2 cut(s) 142, 755
BspCNI CTCAG 1 cut(s) 100
BspMAI CTGCAG 1 cut(s) 463
BspOI GCTAGC 1 cut(s) 570
BspPI GGATC 2 cut(s) 434, 683
BsrDI GCAATG 2 cut(s) 363, 553
BsrI ACTGG 1 cut(s) 742
BssECI CCNNGG 3 cut(s) 285, 631, 760
BssMI GATC 3 cut(s) 25, 439, 675
BssNAI GTATAC 2 cut(s) 341, 619
BssT1I CCWWGG 2 cut(s) 285, 631
Bst1107I GTATAC 2 cut(s) 341, 619
Bst2UI CCWGG 1 cut(s) 811
Bst4CI ACNGT 2 cut(s) 39, 324
BstC8I GCNNGC 3 cut(s) 225, 412, 568
BstDEI CTNAG 2 cut(s) 108, 430
BstDSI CCRYGG 1 cut(s) 760
BstENI CCTNNNNNAGG 1 cut(s) 92
BstF5I GGATG 3 cut(s) 10, 103, 336
BstH2I RGCGCY 1 cut(s) 285
BstHHI GCGC 1 cut(s) 284
BstKTI GATC 3 cut(s) 28, 442, 678
BstMBI GATC 3 cut(s) 25, 439, 675
BstMWI GCNNNNNNNGC 2 cut(s) 239, 653
BstNI CCWGG 1 cut(s) 811
BstNSI RCATGY 1 cut(s) 65
BstSCI CCNGG 1 cut(s) 809
BstSFI CTRYAG 1 cut(s) 459
BstV1I GCAGC 3 cut(s) 136, 445, 634
BstX2I RGATCY 1 cut(s) 439
BstYI RGATCY 1 cut(s) 439
BstZ17I GTATAC 2 cut(s) 341, 619
BsuRI GGCC 2 cut(s) 142, 755
BtgI CCRYGG 1 cut(s) 760
BtsCI GGATG 3 cut(s) 10, 103, 336
BtsI GCAGTG 1 cut(s) 263
BtsIMutI CAGTG 3 cut(s) 35, 263, 431
Cac8I GCNNGC 3 cut(s) 225, 412, 568
CaiI CAGNNNCTG 2 cut(s) 265, 578
CfoI GCGC 1 cut(s) 284
CviAII CATG 4 cut(s) 62, 193, 521, 732
CviJI RGCY 8 cut(s) 127, 142, 217, 242, 404, 630, 755, 800
CviKI_1 RGCY 8 cut(s) 127, 142, 217, 242, 404, 630, 755, 800
DdeI CTNAG 2 cut(s) 108, 430
DpnI GATC 3 cut(s) 27, 441, 677
DpnII GATC 3 cut(s) 25, 439, 675
EaeI YGGCCR 1 cut(s) 140
Eco130I CCWWGG 2 cut(s) 285, 631
Eco147I AGGCCT 1 cut(s) 755
Eco32I GATATC 1 cut(s) 450
Eco57I CTGAAG 2 cut(s) 242, 407
EcoNI CCTNNNNNAGG 1 cut(s) 92
EcoRI GAATTC 1 cut(s) 765
EcoRII CCWGG 1 cut(s) 809
EcoRV GATATC 1 cut(s) 450
EcoT14I CCWWGG 2 cut(s) 285, 631
EcoT22I ATGCAT 1 cut(s) 685
ErhI CCWWGG 2 cut(s) 285, 631
FaeI CATG 4 cut(s) 65, 196, 524, 735
FalI AAGNNNNNCTT 2 cut(s) 740, 772
FatI CATG 4 cut(s) 61, 192, 520, 731
FauNDI CATATG 1 cut(s) 345
FblI GTMKAC 2 cut(s) 340, 618
Fnu4HI GCNGC 4 cut(s) 125, 143, 459, 648
FokI GGATG 3 cut(s) 17, 110, 343
Fsp4HI GCNGC 4 cut(s) 125, 143, 459, 648
FspBI CTAG 2 cut(s) 525, 567
GlaI GCGC 1 cut(s) 283
GluI GCNGC 4 cut(s) 125, 143, 459, 648
HaeII RGCGCY 1 cut(s) 285
HaeIII GGCC 2 cut(s) 142, 755
HhaI GCGC 1 cut(s) 284
Hin1II CATG 4 cut(s) 65, 196, 524, 735
Hin6I GCGC 1 cut(s) 282
HinP1I GCGC 1 cut(s) 282
HinfI GANTC 7 cut(s) 32, 43, 71, 84, 528, 553, 741
HphI GGTGA 1 cut(s) 658
Hpy166II GTNNAC 2 cut(s) 341, 619
Hpy188I TCNGA 3 cut(s) 109, 330, 707
Hpy188III TCNNGA 6 cut(s) 29, 56, 75, 88, 437, 443
Hpy8I GTNNAC 2 cut(s) 341, 619
HpyAV CCTTC 2 cut(s) 766, 804
HpyCH4III ACNGT 2 cut(s) 39, 324
HpyF10VI GCNNNNNNNGC 2 cut(s) 239, 653
HpyF3I CTNAG 2 cut(s) 108, 430
Hsp92II CATG 4 cut(s) 65, 196, 524, 735
HspAI GCGC 1 cut(s) 282
Kzo9I GATC 3 cut(s) 25, 439, 675
LmnI GCTCC 1 cut(s) 314
Lsp1109I GCAGC 3 cut(s) 136, 445, 634
LweI GCATC 1 cut(s) 321
MaeI CTAG 2 cut(s) 525, 567
MaeIII GTNAC 1 cut(s) 261
MalI GATC 3 cut(s) 27, 441, 677
MboI GATC 3 cut(s) 25, 439, 675
MboII GAAGA 4 cut(s) 23, 26, 130, 413
MflI RGATCY 1 cut(s) 439
MluCI AATT 5 cut(s) 49, 157, 183, 393, 765
MnlI CCTC 8 cut(s) 103, 345, 396, 458, 463, 635, 727, 745
Mph1103I ATGCAT 1 cut(s) 685
MseI TTAA 2 cut(s) 134, 803
MspA1I CMGCKG 2 cut(s) 127, 300
MspR9I CCNGG 1 cut(s) 811
MvaI CCWGG 1 cut(s) 811
MwoI GCNNNNNNNGC 2 cut(s) 239, 653
NdeI CATATG 1 cut(s) 345
NdeII GATC 3 cut(s) 25, 439, 675
NheI GCTAGC 1 cut(s) 566
NlaIII CATG 4 cut(s) 65, 196, 524, 735
NmuCI GTSAC 1 cut(s) 261
NsiI ATGCAT 1 cut(s) 685
NspI RCATGY 1 cut(s) 65
PceI AGGCCT 1 cut(s) 755
PfeI GAWTC 7 cut(s) 32, 43, 71, 84, 528, 553, 741
PkrI GCNGC 4 cut(s) 126, 144, 460, 649
Psp6I CCWGG 1 cut(s) 809
PspGI CCWGG 1 cut(s) 809
PstI CTGCAG 1 cut(s) 463
PstNI CAGNNNCTG 2 cut(s) 265, 578
PsuI RGATCY 1 cut(s) 439
PvuII CAGCTG 1 cut(s) 127
SaqAI TTAA 2 cut(s) 134, 803
SatI GCNGC 4 cut(s) 125, 143, 459, 648
Sau3AI GATC 3 cut(s) 25, 439, 675
ScrFI CCNGG 1 cut(s) 811
SetI ASST 9 cut(s) 129, 219, 436, 469, 490, 605, 646, 671, 815
SfaNI GCATC 1 cut(s) 321
SfcI CTRYAG 1 cut(s) 459
SmlI CTYRAG 1 cut(s) 54
SmoI CTYRAG 1 cut(s) 54
Sse9I AATT 5 cut(s) 49, 157, 183, 393, 765
SseBI AGGCCT 1 cut(s) 755
SsiI CCGC 2 cut(s) 143, 300
SspI AATATT 1 cut(s) 418
SspMI CTAG 2 cut(s) 525, 567
StuI AGGCCT 1 cut(s) 755
StyD4I CCNGG 1 cut(s) 809
StyI CCWWGG 2 cut(s) 285, 631
TaaI ACNGT 2 cut(s) 39, 324
TaqI TCGA 1 cut(s) 74
TasI AATT 5 cut(s) 49, 157, 183, 393, 765
TauI GCSGC 1 cut(s) 145
TfiI GAWTC 7 cut(s) 32, 43, 71, 84, 528, 553, 741
Tru1I TTAA 2 cut(s) 134, 803
Tru9I TTAA 2 cut(s) 134, 803
TscAI CASTG 3 cut(s) 42, 270, 431
TseFI GTSAC 1 cut(s) 261
TseI GCWGC 3 cut(s) 124, 458, 647
Tsp45I GTSAC 1 cut(s) 261
TspDTI ATGAA 1 cut(s) 239
TspGWI ACGGA 1 cut(s) 777
TspRI CASTG 3 cut(s) 42, 270, 431
XagI CCTNNNNNAGG 1 cut(s) 92
XapI RAATTY 2 cut(s) 157, 765
XceI RCATGY 1 cut(s) 65
XmiI GTMKAC 2 cut(s) 340, 618
XspI CTAG 2 cut(s) 525, 567
Zsp2I ATGCAT 1 cut(s) 685
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.