FvH4_4g27904

Mitochondrial protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0001471
Physical Location & Seq
Forward (+)
28699517 .. 28701415
1899 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g27904.t1

Sequence Viewer

Length: 1899 bp
ATGTCTTCACAAAATAATGATTCTTCTCCTAGCCAAGCCTTTGATCAGTCTGCAAACGCTGAAATTATTTTTGAAGAGTCAAATGGGGAGTTGTCTGTCCAATCTGAGCCTGGTGCTGGATCCATCTCTCTTGTTCTCAATTCTGAACCCTCCAGACCAACTCATCAAATCTCTGATGCCAACATCCTACATCCAGTTGCTGGTCTCACTCCAACTACTGAAAATGAGCATCCAATGCTCACCAAAAGCAAAAGAGGAATAGTACAGAAAAAGTGTTTTCTCTCAATCATGAATCAAGACACTTCTAATCCTTCAGAAATTGAACCCTACTCAGTCAAGACAGCTCTACAAGTTTCAGTATGGAAGAATGCAATGCAGGAGGAGTATGATGCTCTCATGAAACAGCAGACTTGGTCCTTGGTTCCTTTGCCTCCAGATAAAAATCTTGTGCCCTGTAAATGGATTTTCAAAATCAAGAGAAATGCAGATGGTTCAATTGCCAGAGATAAAGCAAGATTAGTGGCGAGGGGTTTCAGTCAAGAGTATGGCATAGATTATGAAGAAACCTTCTCTCCAGTTGTAAGGCACACAACAGTTCAACTAATTTTAGCATATGCTACATATTCTGGTTGGATCCTGCATCAACTTGATGTGAAAAATGCCTTCCTTCATGGAGTGTTAAGTGAAGAAGTATATATGGCTCAATCAAGTGGTTTTGTAGTTGCCCCTGCAACACATGTGTGTAAACTACACAAATCTCTTTATGGATTGAAACAAGTTCCTAGAGCTTGGAATGAAAGGTTCACAACTTTCCTTCCTAAACTTGGTTTCTCCCTTTCATATGCAGATCCTAGTCTGTTTGTGTTAATCATAGGAACTTCAAGAGTGTATCTTCTATTATATGTTGATGATATCATTGTGACTGGCAACAGTGAGACTCTTATTGATGAAATCAAAGTTTCTTTACAGCAAGAATTTGACATGAAGGATCTAGGTCCACTGCACTTCTTCCTTGGTCTTGAAATCAAATATTTGAAGAATGGTGGAATTTTCATATCTCAGCAGAAGTATGCAACGGATCTTATCCATAAAGCAGGTCTTGGACACTGTCATACTCATTTGACACCATGTCAGTCTGGAGTGAAACTTTTCTTAGAGAATGGGAAAACAATAGACTCATCCGAAGGCCCTCATTTCAGAAGCTTGGTTGGCTGTCTTCAATGCTTAACATTCACAAGGCCAGATATAACATATGCTGTGAACTCAGTTTGTCAATTTATGCATAATCCATCTTCTGAACATCTTATTGCAGCAAAAAGAATACTTAGATATGTTAAGGGCACCATTGATTATGGTATTTGGTTCAGGAGGGGTCCTTCACTTACTGATTCTCCAGCACTCCCAATTCAGCTCGATGCCTTTTGTGATGCAGATTGGGCAGGTGACCCAAATGACAGGAAATCAACCACTGGCTTTGTAATTCTTTTGAACGATGCTCCCATCTCTTGGTGCAGCAAAAAACAAAATGCAGTGTCAAGGTCCTCCACTAAAGCTGAGTATAGGAGCATGGCTGACACAACTTCAGAGTTCATGTGGCTCTCTCATCTGCTAAAAGACTTGCACATTGTTCACAATCAAATTCCTACTCTCCACTGTGACAACATATCAGCTCTTGCTCTAGCAACAAATCCTGTATACCACTCCAAGCTGAAACACGTTGAAGTTGATGTTCACTTCACCAGAAACCAAGTCAAGGCAAGAACAATCAGAGTGCAATTCATCTCCTCTAAAGAACAACTTGCTAACCTCTTTACAAAAGGACTGTGCTCACCACAACACAATTATCTTTGTGACAGCTTGAAGTTGCTTCCACACCATCAAGCTGAGGAGGGGTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2

Protein Analysis

633

Amino Acids

70.8

Weight (kDa)

6.81

Isoelectric Point (pI)

48.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_2 PF07727 136 - 377 1.4e-70 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1430
Acc36I ACCTGC 2 cut(s) 1085, 1430
AccB1I GGYRCC 1 cut(s) 1340
AccB7I CCANNNNNTGG 2 cut(s) 200, 1506
AccI GTMKAC 1 cut(s) 1695
AclWI GGATC 7 cut(s) 114, 127, 628, 641, 842, 996, 1086
AcsI RAATTY 3 cut(s) 974, 1047, 1638
AcuI CTGAAG 2 cut(s) 297, 1566
AfaI GTAC 1 cut(s) 264
AfiI CCNNNNNNNGG 6 cut(s) 116, 200, 459, 824, 1506, 1753
AflIII ACRYGT 2 cut(s) 736, 1714
AhdI GACNNNNNGTC 1 cut(s) 1128
AjnI CCWGG 1 cut(s) 109
AleI CACNNNNGTG 1 cut(s) 739
AluBI AGCT 9 cut(s) 344, 788, 1203, 1411, 1553, 1670, 1708, 1857, 1883
AluI AGCT 9 cut(s) 344, 788, 1203, 1411, 1553, 1670, 1708, 1857, 1883
Alw21I GWGCWC 1 cut(s) 1829
Alw26I GTCTC 2 cut(s) 209, 929
AlwI GGATC 7 cut(s) 114, 127, 628, 641, 842, 996, 1086
AlwNI CAGNNNCTG 1 cut(s) 200
AoxI GGCC 2 cut(s) 1186, 1238
ApeKI GCWGC 2 cut(s) 1310, 1512
ApoI RAATTY 3 cut(s) 974, 1047, 1638
Asp700I GAANNNNTTC 1 cut(s) 1148
AspS9I GGNCC 5 cut(s) 414, 995, 1187, 1373, 1539
AsuHPI GGTGA 4 cut(s) 232, 1454, 1729, 1821
AvaII GGWCC 4 cut(s) 414, 995, 1373, 1539
BaeGI GKGCMC 2 cut(s) 453, 1343
BamHI GGATCC 2 cut(s) 119, 633
BanI GGYRCC 1 cut(s) 1340
BbsI GAAGAC 1 cut(s) 1208
Bbv12I GWGCWC 1 cut(s) 1829
BbvCI CCTCAGC 1 cut(s) 1884
BbvI GCAGC 2 cut(s) 1322, 1524
BccI CCATC 5 cut(s) 131, 482, 1297, 1508, 1884
BciT130I CCWGG 1 cut(s) 111
BclI TGATCA 1 cut(s) 43
BcoDI GTCTC 2 cut(s) 209, 929
BfaI CTAG 5 cut(s) 30, 783, 852, 992, 1679
BfuAI ACCTGC 2 cut(s) 1085, 1430
BisI GCNGC 2 cut(s) 1311, 1513
BlsI GCNGC 2 cut(s) 1312, 1514
Bme1390I CCNGG 1 cut(s) 111
Bme18I GGWCC 4 cut(s) 414, 995, 1373, 1539
BmeRI GACNNNNNGTC 1 cut(s) 1128
BmgT120I GGNCC 5 cut(s) 414, 995, 1187, 1373, 1539
BmiI GGNNCC 5 cut(s) 121, 423, 635, 1342, 1374
BmrFI CCNGG 1 cut(s) 111
BmsI GCATC 7 cut(s) 166, 238, 379, 649, 1405, 1417, 1483
BpiI GAAGAC 1 cut(s) 1208
BpmI CTGGAG 5 cut(s) 136, 417, 558, 1158, 1377
Bpu10I CCTNAGC 1 cut(s) 1884
BsaBI GATNNNNATC 1 cut(s) 441
BsaI GGTCTC 1 cut(s) 209
BsaJI CCNNGG 2 cut(s) 417, 1012
BsaXI ACNNNNNCTCC 4 cut(s) 556, 586, 1375, 1405
Bsc4I CCNNNNNNNGG 6 cut(s) 116, 200, 459, 824, 1506, 1753
Bse1I ACTGG 4 cut(s) 194, 575, 928, 1474
Bse3DI GCAATG 1 cut(s) 378
Bse8I GATNNNNATC 1 cut(s) 441
BseBI CCWGG 1 cut(s) 111
BseDI CCNNGG 2 cut(s) 417, 1012
BseGI GGATG 4 cut(s) 183, 190, 229, 1178
BseJI GATNNNNATC 1 cut(s) 441
BseLI CCNNNNNNNGG 6 cut(s) 116, 200, 459, 824, 1506, 1753
BseMI GCAATG 1 cut(s) 378
BseMII CTCAG 6 cut(s) 96, 345, 1073, 1278, 1545, 1875
BseNI ACTGG 4 cut(s) 194, 575, 928, 1474
BseRI GAGGAG 2 cut(s) 395, 1774
BseSI GKGCMC 2 cut(s) 453, 1343
BseXI GCAGC 2 cut(s) 1322, 1524
BsgI GTGCAG 2 cut(s) 986, 1531
BshFI GGCC 2 cut(s) 1188, 1240
BshNI GGYRCC 1 cut(s) 1340
BsiHKAI GWGCWC 1 cut(s) 1829
BslI CCNNNNNNNGG 6 cut(s) 116, 200, 459, 824, 1506, 1753
BsmAI GTCTC 2 cut(s) 209, 929
BsmI GAATGC 1 cut(s) 373
BsnI GGCC 2 cut(s) 1188, 1240
Bso31I GGTCTC 1 cut(s) 209
Bsp1286I GDGCHC 3 cut(s) 453, 1343, 1829
Bsp143I GATC 6 cut(s) 43, 119, 633, 847, 988, 1078
BspANI GGCC 2 cut(s) 1188, 1240
BspCNI CTCAG 6 cut(s) 97, 344, 1072, 1277, 1546, 1876
BspHI TCATGA 2 cut(s) 288, 396
BspLI GGNNCC 5 cut(s) 121, 423, 635, 1342, 1374
BspMI ACCTGC 2 cut(s) 1085, 1430
BspPI GGATC 7 cut(s) 114, 127, 628, 641, 842, 996, 1086
BspT107I GGYRCC 1 cut(s) 1340
BspTNI GGTCTC 1 cut(s) 209
BsrDI GCAATG 1 cut(s) 378
BsrI ACTGG 4 cut(s) 194, 575, 928, 1474
BssECI CCNNGG 2 cut(s) 417, 1012
BssMI GATC 6 cut(s) 43, 119, 633, 847, 988, 1078
BssNAI GTATAC 1 cut(s) 1696
BssT1I CCWWGG 2 cut(s) 417, 1012
Bst1107I GTATAC 1 cut(s) 1696
Bst2UI CCWGG 1 cut(s) 111
Bst4CI ACNGT 5 cut(s) 595, 932, 1109, 1655, 1824
Bst6I CTCTTC 1 cut(s) 69
BstAPI GCANNNNNTGC 1 cut(s) 235
BstDEI CTNAG 8 cut(s) 105, 331, 1059, 1153, 1264, 1325, 1554, 1884
BstEII GGTNACC 1 cut(s) 1442
BstF5I GGATG 4 cut(s) 183, 190, 229, 1178
BstKTI GATC 6 cut(s) 46, 122, 636, 850, 991, 1081
BstMAI GTCTC 2 cut(s) 209, 929
BstMBI GATC 6 cut(s) 43, 119, 633, 847, 988, 1078
BstMWI GCNNNNNNNGC 3 cut(s) 235, 1209, 1436
BstNI CCWGG 1 cut(s) 111
BstNSI RCATGY 1 cut(s) 740
BstPI GGTNACC 1 cut(s) 1442
BstSCI CCNGG 1 cut(s) 109
BstSLI GKGCMC 2 cut(s) 453, 1343
BstV1I GCAGC 2 cut(s) 1322, 1524
BstV2I GAAGAC 1 cut(s) 1208
BstX2I RGATCY 5 cut(s) 119, 633, 847, 988, 1078
BstYI RGATCY 5 cut(s) 119, 633, 847, 988, 1078
BstZ17I GTATAC 1 cut(s) 1696
BsuRI GGCC 2 cut(s) 1188, 1240
BtsCI GGATG 4 cut(s) 183, 190, 229, 1178
BtsI GCAGTG 2 cut(s) 998, 1536
BtsIMutI CAGTG 6 cut(s) 937, 998, 1105, 1467, 1536, 1651
BveI ACCTGC 2 cut(s) 1085, 1430
CaiI CAGNNNCTG 1 cut(s) 200
CciI TCATGA 2 cut(s) 288, 396
Cfr13I GGNCC 5 cut(s) 414, 995, 1187, 1373, 1539
Csp6I GTAC 1 cut(s) 263
CspCI CAANNNNNGTGG 4 cut(s) 501, 536, 1456, 1491
CviAII CATG 8 cut(s) 289, 397, 671, 737, 982, 1128, 1567, 1591
CviQI GTAC 1 cut(s) 263
DdeI CTNAG 8 cut(s) 105, 331, 1059, 1153, 1264, 1325, 1554, 1884
DpnI GATC 6 cut(s) 45, 121, 635, 849, 990, 1080
DpnII GATC 6 cut(s) 43, 119, 633, 847, 988, 1078
DriI GACNNNNNGTC 1 cut(s) 1128
Eam1104I CTCTTC 1 cut(s) 69
Eam1105I GACNNNNNGTC 1 cut(s) 1128
EarI CTCTTC 1 cut(s) 69
Eco130I CCWWGG 2 cut(s) 417, 1012
Eco31I GGTCTC 1 cut(s) 209
Eco32I GATATC 1 cut(s) 913
Eco47I GGWCC 4 cut(s) 414, 995, 1373, 1539
Eco57I CTGAAG 2 cut(s) 297, 1566
Eco91I GGTNACC 1 cut(s) 1442
EcoO109I RGGNCCY 3 cut(s) 1187, 1373, 1539
EcoO65I GGTNACC 1 cut(s) 1442
EcoRII CCWGG 1 cut(s) 109
EcoRV GATATC 1 cut(s) 913
EcoT14I CCWWGG 2 cut(s) 417, 1012
EcoT22I ATGCAT 1 cut(s) 1284
ErhI CCWWGG 2 cut(s) 417, 1012
FaeI CATG 8 cut(s) 292, 400, 674, 740, 985, 1131, 1570, 1594
FalI AAGNNNNNCTT 4 cut(s) 1083, 1115, 1782, 1814
FatI CATG 8 cut(s) 288, 396, 670, 736, 981, 1127, 1566, 1590
FauNDI CATATG 3 cut(s) 613, 841, 1252
FbaI TGATCA 1 cut(s) 43
FblI GTMKAC 1 cut(s) 1695
Fnu4HI GCNGC 2 cut(s) 1311, 1513
FokI GGATG 4 cut(s) 170, 177, 216, 1165
Fsp4HI GCNGC 2 cut(s) 1311, 1513
FspBI CTAG 5 cut(s) 30, 783, 852, 992, 1679
GluI GCNGC 2 cut(s) 1311, 1513
GsuI CTGGAG 5 cut(s) 136, 417, 558, 1158, 1377
HaeIII GGCC 2 cut(s) 1188, 1240
Hin1II CATG 8 cut(s) 292, 400, 674, 740, 985, 1131, 1570, 1594
HindIII AAGCTT 1 cut(s) 1201
HinfI GANTC 6 cut(s) 20, 77, 292, 937, 1175, 1388
HphI GGTGA 4 cut(s) 232, 1454, 1729, 1821
Hpy166II GTNNAC 7 cut(s) 746, 804, 998, 1261, 1630, 1696, 1732
Hpy188I TCNGA 9 cut(s) 106, 145, 175, 316, 1183, 1199, 1297, 1585, 1769
Hpy8I GTNNAC 7 cut(s) 746, 804, 998, 1261, 1630, 1696, 1732
HpyAV CCTTC 8 cut(s) 321, 577, 673, 677, 824, 979, 1178, 1386
HpyCH4III ACNGT 5 cut(s) 595, 932, 1109, 1655, 1824
HpyCH4IV ACGT 1 cut(s) 1716
HpyF10VI GCNNNNNNNGC 3 cut(s) 235, 1209, 1436
HpyF3I CTNAG 8 cut(s) 105, 331, 1059, 1153, 1264, 1325, 1554, 1884
HpySE526I ACGT 1 cut(s) 1716
Hsp92II CATG 8 cut(s) 292, 400, 674, 740, 985, 1131, 1570, 1594
Ksp22I TGATCA 1 cut(s) 43
Kzo9I GATC 6 cut(s) 43, 119, 633, 847, 988, 1078
LmnI GCTCC 2 cut(s) 1501, 1563
Lsp1109I GCAGC 2 cut(s) 1322, 1524
LweI GCATC 7 cut(s) 166, 238, 379, 649, 1405, 1417, 1483
MaeI CTAG 5 cut(s) 30, 783, 852, 992, 1679
MaeII ACGT 1 cut(s) 1716
MaeIII GTNAC 4 cut(s) 919, 1442, 1655, 1850
MalI GATC 6 cut(s) 45, 121, 635, 849, 990, 1080
MboI GATC 6 cut(s) 43, 119, 633, 847, 988, 1078
MfeI CAATTG 1 cut(s) 495
MflI RGATCY 5 cut(s) 119, 633, 847, 988, 1078
MhlI GDGCHC 3 cut(s) 453, 1343, 1829
MlyI GAGTC 3 cut(s) 86, 931, 1169
MmeI TCCRAC 2 cut(s) 236, 611
Mph1103I ATGCAT 1 cut(s) 1284
MroXI GAANNNNTTC 1 cut(s) 1148
MseI TTAA 4 cut(s) 680, 866, 1226, 1335
MslI CAYNNNNRTG 1 cut(s) 739
MspR9I CCNGG 1 cut(s) 111
MunI CAATTG 1 cut(s) 495
Mva1269I GAATGC 1 cut(s) 373
MvaI CCWGG 1 cut(s) 111
MwoI GCNNNNNNNGC 3 cut(s) 235, 1209, 1436
NdeI CATATG 3 cut(s) 613, 841, 1252
NdeII GATC 6 cut(s) 43, 119, 633, 847, 988, 1078
NlaIII CATG 8 cut(s) 292, 400, 674, 740, 985, 1131, 1570, 1594
NlaIV GGNNCC 5 cut(s) 121, 423, 635, 1342, 1374
NmuCI GTSAC 4 cut(s) 919, 1442, 1655, 1850
NsiI ATGCAT 1 cut(s) 1284
NspI RCATGY 1 cut(s) 740
OliI CACNNNNGTG 1 cut(s) 739
PagI TCATGA 2 cut(s) 288, 396
PaqCI CACCTGC 1 cut(s) 1430
PciI ACATGT 1 cut(s) 736
PctI GAATGC 1 cut(s) 373
PdmI GAANNNNTTC 1 cut(s) 1148
PfeI GAWTC 3 cut(s) 20, 292, 1388
PflFI GACNNNGTC 2 cut(s) 412, 1107
PflMI CCANNNNNTGG 2 cut(s) 200, 1506
PkrI GCNGC 2 cut(s) 1312, 1514
PleI GAGTC 3 cut(s) 85, 931, 1169
PpsI GAGTC 3 cut(s) 85, 931, 1169
PpuMI RGGWCCY 2 cut(s) 1373, 1539
PscI ACATGT 1 cut(s) 736
Psp5II RGGWCCY 2 cut(s) 1373, 1539
Psp6I CCWGG 1 cut(s) 109
PspEI GGTNACC 1 cut(s) 1442
PspGI CCWGG 1 cut(s) 109
PspN4I GGNNCC 5 cut(s) 121, 423, 635, 1342, 1374
PspPI GGNCC 5 cut(s) 414, 995, 1187, 1373, 1539
PspPPI RGGWCCY 2 cut(s) 1373, 1539
PstNI CAGNNNCTG 1 cut(s) 200
PsuI RGATCY 5 cut(s) 119, 633, 847, 988, 1078
PsyI GACNNNGTC 2 cut(s) 412, 1107
RsaI GTAC 1 cut(s) 264
RsaNI GTAC 1 cut(s) 263
RseI CAYNNNNRTG 1 cut(s) 739
SaqAI TTAA 4 cut(s) 680, 866, 1226, 1335
SatI GCNGC 2 cut(s) 1311, 1513
Sau3AI GATC 6 cut(s) 43, 119, 633, 847, 988, 1078
Sau96I GGNCC 5 cut(s) 414, 995, 1187, 1373, 1539
SchI GAGTC 3 cut(s) 86, 931, 1169
ScrFI CCNGG 1 cut(s) 111
SduI GDGCHC 3 cut(s) 453, 1343, 1829
SfaNI GCATC 7 cut(s) 166, 238, 379, 649, 1405, 1417, 1483
SinI GGWCC 4 cut(s) 414, 995, 1373, 1539
SmiMI CAYNNNNRTG 1 cut(s) 739
SspI AATATT 1 cut(s) 1031
SspMI CTAG 5 cut(s) 30, 783, 852, 992, 1679
StyD4I CCNGG 1 cut(s) 109
StyI CCWWGG 2 cut(s) 417, 1012
TaaI ACNGT 5 cut(s) 595, 932, 1109, 1655, 1824
TaiI ACGT 1 cut(s) 1719
TaqI TCGA 1 cut(s) 1413
TatI WGTACW 1 cut(s) 262
TfiI GAWTC 3 cut(s) 20, 292, 1388
Tru1I TTAA 4 cut(s) 680, 866, 1226, 1335
Tru9I TTAA 4 cut(s) 680, 866, 1226, 1335
TscAI CASTG 6 cut(s) 937, 1005, 1112, 1474, 1536, 1658
TseFI GTSAC 4 cut(s) 919, 1442, 1655, 1850
TseI GCWGC 2 cut(s) 1310, 1512
Tsp45I GTSAC 4 cut(s) 919, 1442, 1655, 1850
TspGWI ACGGA 1 cut(s) 1091
TspRI CASTG 6 cut(s) 937, 1005, 1112, 1474, 1536, 1658
Tth111I GACNNNGTC 2 cut(s) 412, 1107
Van91I CCANNNNNTGG 2 cut(s) 200, 1506
VpaK11BI GGWCC 4 cut(s) 414, 995, 1373, 1539
XapI RAATTY 3 cut(s) 974, 1047, 1638
XceI RCATGY 1 cut(s) 740
XcmI CCANNNNNNNNNTGG 1 cut(s) 107
XmiI GTMKAC 1 cut(s) 1695
XmnI GAANNNNTTC 1 cut(s) 1148
XspI CTAG 5 cut(s) 30, 783, 852, 992, 1679
Zsp2I ATGCAT 1 cut(s) 1284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.