RchiOBHm_Chr4g0401001
RLK Family

cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0001471
Physical Location & Seq
Forward (+)
18969829 .. 18972501
2673 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37299

Sequence Viewer

Length: 1272 bp
ATGGAGAAGGACTATTTTTGTTCAAATCTCCAAGATTCATTCCACCTGCTCATCACACACATACTGCCTTTATTGGTTCTGCTGTCAAGTCTTCTCTTTGGCATCAAAGACTTGGTCATCCTACCTCAAGAGTTGCAATTTGTTGAAGATCAGTCTCAGGCATATAATAATCACTCTATGCTTACAAGGGCTAAGAATGGCATTGTGAAGAAGAAGTCATTTCAAGACTTTTGTGCCTTCTCCTGTATTGTTCAACAAAATCTACTTGATGAACTTGCATTTTTTAGTGGTTTCACTTCAATTCTTGAAGTTCATGATCCTATTGAGCCTAAGTCCTTTAAGGCTGCTGTTGGCAAACCAGAATGGGATATTGCTATGAATGAAGAAATTGATGCACTACTTAAACAAGGTACTTGGAAACTTGTTCCTCTTCCAAAAGATAAAAACATTGTTGGCAGCAAGTGGATTTACAAGGTTAAAAAGAATCCAGATGGGACTGTCTCAAGGTATAAGGCCAGGCTTGTGGCTCAAGGTTTTAGTCAAGAGAAAGGGTTGGATTATGACGAAACATTTAGTCCTGTTGTGAGACACAGTACCGTGAGGTTGGTGATTGCTCTTGCAGCTATGAATAGGTGGGAGTTGAGACAATTGGATGTCAAAAATGCCTTTTTGCATGGTGATCTCAGGGAAGAAGTATACATGCAACAACCAATTGGTTTTACTGATCCTGTACATCCTGAGTATGTATGCCTACTCAAAAAATCTCTTTATGGTCTAAAACAGGCACCTAGGGCCTGGAATGAGAAGTTCTCTAATTTTCTTCCAGCCTTAGGCTTTCATTTTTCTCACTCGGATCCCAGCTTGTTTATCAAGAACAGTGAGAAAGGTGTTGTAGCATTATTGTTATACGTGGATGATATTATTGTTACAGGATCAGATACTGAAGGAATTATTGCTGTAATATCTGAGCTAGGTGAAGTATTTGACATGAAAGATTTAGGATCTTTGTCTTACTTCCTTGGTATTAATGTGCAGTATAAGAAGAAAGGCATTTTTCTGTCTCAAGAAAAATATGCTAAAGAGTTAATTCTTAAGGCAGGTCTGGAGTCTTGTAAAGCATGTAGTTCACCTTGTCTTCCTCATATTCAGCTGCTTAAGAGTGAAGGAACTTTATTGGATAATCCCACACTTTACAGAAGCTTGGTCGGTGCATTGCAGTACCTCACATTTACCAGACCAGATATTGCATACGCAGTTAATACAGTTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2 Retrotran_gag_3 Retrotrans_gag gag_pre-integrs rve

Protein Analysis

423

Amino Acids

47.73

Weight (kDa)

6.57

Isoelectric Point (pI)

39.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_2 PF07727 138 - 379 1.3e-75 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 54
Acc36I ACCTGC 2 cut(s) 54, 1088
AccB1I GGYRCC 1 cut(s) 784
AccI GTMKAC 1 cut(s) 696
AclWI GGATC 6 cut(s) 311, 719, 848, 861, 940, 1009
AcuI CTGAAG 1 cut(s) 963
AfaI GTAC 4 cut(s) 412, 595, 732, 1220
AfiI CCNNNNNNNGG 1 cut(s) 830
AflII CTTAAG 2 cut(s) 1091, 1154
AgsI TTSAA 6 cut(s) 24, 146, 224, 254, 300, 308
AjnI CCWGG 2 cut(s) 515, 794
AluBI AGCT 5 cut(s) 623, 861, 970, 1150, 1200
AluI AGCT 5 cut(s) 623, 861, 970, 1150, 1200
Alw26I GTCTC 5 cut(s) 159, 505, 580, 637, 1065
AlwI GGATC 6 cut(s) 311, 719, 848, 861, 940, 1009
AlwNI CAGNNNCTG 1 cut(s) 941
AoxI GGCC 2 cut(s) 513, 792
ApeKI GCWGC 4 cut(s) 344, 456, 620, 1150
ArsI GACNNNNNNTTYG 2 cut(s) 99, 131
AseI ATTAAT 1 cut(s) 1026
AspA2I CCTAGG 1 cut(s) 788
AspS9I GGNCC 1 cut(s) 792
AsuHPI GGTGA 4 cut(s) 619, 689, 986, 1119
AvrII CCTAGG 1 cut(s) 788
AxyI CCTNAGG 1 cut(s) 829
BamHI GGATCC 1 cut(s) 853
BanI GGYRCC 1 cut(s) 784
BbsI GAAGAC 2 cut(s) 83, 1127
BbvI GCAGC 4 cut(s) 331, 468, 632, 1137
BccI CCATC 1 cut(s) 485
BciT130I CCWGG 2 cut(s) 517, 796
BcoDI GTCTC 5 cut(s) 159, 505, 580, 637, 1065
BfaI CTAG 2 cut(s) 789, 971
BfrI CTTAAG 2 cut(s) 1091, 1154
BfuAI ACCTGC 2 cut(s) 54, 1088
BisI GCNGC 4 cut(s) 345, 457, 621, 1151
BlnI CCTAGG 1 cut(s) 788
BlsI GCNGC 4 cut(s) 346, 458, 622, 1152
Bme1390I CCNGG 2 cut(s) 517, 796
BmgT120I GGNCC 1 cut(s) 792
BmiI GGNNCC 2 cut(s) 786, 855
BmrFI CCNGG 2 cut(s) 517, 796
BmsI GCATC 2 cut(s) 111, 382
BpiI GAAGAC 2 cut(s) 83, 1127
BplI GAGNNNNNCTC 2 cut(s) 794, 826
BpmI CTGGAG 1 cut(s) 1124
BpuEI CTTGAG 4 cut(s) 111, 487, 513, 1047
BsaAI YACGTR 1 cut(s) 910
BsaJI CCNNGG 2 cut(s) 788, 1018
Bsc4I CCNNNNNNNGG 1 cut(s) 830
Bse21I CCTNAGG 1 cut(s) 829
Bse3DI GCAATG 1 cut(s) 1211
BseBI CCWGG 2 cut(s) 517, 796
BseDI CCNNGG 2 cut(s) 788, 1018
BseGI GGATG 4 cut(s) 117, 658, 733, 919
BseLI CCNNNNNNNGG 1 cut(s) 830
BseMI GCAATG 1 cut(s) 1211
BseMII CTCAG 4 cut(s) 170, 697, 729, 957
BseXI GCAGC 4 cut(s) 331, 468, 632, 1137
BseYI CCCAGC 1 cut(s) 857
BsgI GTGCAG 1 cut(s) 1052
BshFI GGCC 2 cut(s) 515, 794
BshNI GGYRCC 1 cut(s) 784
BslFI GGGAC 1 cut(s) 508
BslI CCNNNNNNNGG 1 cut(s) 830
BsmAI GTCTC 5 cut(s) 159, 505, 580, 637, 1065
BsmFI GGGAC 1 cut(s) 508
BsnI GGCC 2 cut(s) 515, 794
Bsp1407I TGTACA 1 cut(s) 730
Bsp143I GATC 7 cut(s) 148, 316, 679, 724, 853, 932, 1001
BspANI GGCC 2 cut(s) 515, 794
BspCNI CTCAG 4 cut(s) 169, 696, 730, 958
BspHI TCATGA 1 cut(s) 313
BspLI GGNNCC 2 cut(s) 786, 855
BspMI ACCTGC 2 cut(s) 54, 1088
BspPI GGATC 6 cut(s) 311, 719, 848, 861, 940, 1009
BspT107I GGYRCC 1 cut(s) 784
BspTI CTTAAG 2 cut(s) 1091, 1154
BsrDI GCAATG 1 cut(s) 1211
BsrGI TGTACA 1 cut(s) 730
BssECI CCNNGG 2 cut(s) 788, 1018
BssMI GATC 7 cut(s) 148, 316, 679, 724, 853, 932, 1001
BssNAI GTATAC 1 cut(s) 697
BssT1I CCWWGG 2 cut(s) 788, 1018
Bst1107I GTATAC 1 cut(s) 697
Bst2UI CCWGG 2 cut(s) 517, 796
Bst4CI ACNGT 5 cut(s) 499, 593, 598, 878, 1264
Bst6I CTCTTC 1 cut(s) 435
BstAFI CTTAAG 2 cut(s) 1091, 1154
BstAUI TGTACA 1 cut(s) 730
BstBAI YACGTR 1 cut(s) 910
BstDEI CTNAG 7 cut(s) 156, 192, 330, 683, 738, 829, 966
BstF5I GGATG 4 cut(s) 117, 658, 733, 919
BstKTI GATC 7 cut(s) 151, 319, 682, 727, 856, 935, 1004
BstMAI GTCTC 5 cut(s) 159, 505, 580, 637, 1065
BstMBI GATC 7 cut(s) 148, 316, 679, 724, 853, 932, 1001
BstMWI GCNNNNNNNGC 2 cut(s) 620, 791
BstNI CCWGG 2 cut(s) 517, 796
BstNSI RCATGY 2 cut(s) 703, 1122
BstSCI CCNGG 2 cut(s) 515, 794
BstV1I GCAGC 4 cut(s) 331, 468, 632, 1137
BstV2I GAAGAC 2 cut(s) 83, 1127
BstX2I RGATCY 2 cut(s) 853, 1001
BstXI CCANNNNNNTGG 1 cut(s) 523
BstYI RGATCY 2 cut(s) 853, 1001
BstZ17I GTATAC 1 cut(s) 697
Bsu36I CCTNAGG 1 cut(s) 829
BsuRI GGCC 2 cut(s) 515, 794
BtsCI GGATG 4 cut(s) 117, 658, 733, 919
BtsIMutI CAGTG 1 cut(s) 883
BveI ACCTGC 2 cut(s) 54, 1088
CaiI CAGNNNCTG 1 cut(s) 941
CciI TCATGA 1 cut(s) 313
Cfr13I GGNCC 1 cut(s) 792
Csp6I GTAC 4 cut(s) 411, 594, 731, 1219
CviAII CATG 5 cut(s) 314, 674, 700, 988, 1119
CviQI GTAC 4 cut(s) 411, 594, 731, 1219
DdeI CTNAG 7 cut(s) 156, 192, 330, 683, 738, 829, 966
DpnI GATC 7 cut(s) 150, 318, 681, 726, 855, 934, 1003
DpnII GATC 7 cut(s) 148, 316, 679, 724, 853, 932, 1001
Eam1104I CTCTTC 1 cut(s) 435
EarI CTCTTC 1 cut(s) 435
Eco130I CCWWGG 2 cut(s) 788, 1018
Eco57I CTGAAG 1 cut(s) 963
Eco81I CCTNAGG 1 cut(s) 829
EcoO109I RGGNCCY 1 cut(s) 792
EcoRII CCWGG 2 cut(s) 515, 794
EcoT14I CCWWGG 2 cut(s) 788, 1018
ErhI CCWWGG 2 cut(s) 788, 1018
FaeI CATG 5 cut(s) 317, 677, 703, 991, 1122
FaqI GGGAC 1 cut(s) 508
FatI CATG 5 cut(s) 313, 673, 699, 987, 1118
FblI GTMKAC 1 cut(s) 696
Fnu4HI GCNGC 4 cut(s) 345, 457, 621, 1151
FokI GGATG 4 cut(s) 104, 665, 720, 926
Fsp4HI GCNGC 4 cut(s) 345, 457, 621, 1151
FspBI CTAG 2 cut(s) 789, 971
GluI GCNGC 4 cut(s) 345, 457, 621, 1151
GsaI CCCAGC 1 cut(s) 861
GsuI CTGGAG 1 cut(s) 1124
HaeIII GGCC 2 cut(s) 515, 794
Hin1II CATG 5 cut(s) 317, 677, 703, 991, 1122
HindIII AAGCTT 1 cut(s) 1198
HinfI GANTC 3 cut(s) 35, 484, 1106
HphI GGTGA 4 cut(s) 619, 689, 986, 1119
Hpy166II GTNNAC 2 cut(s) 697, 1127
Hpy188I TCNGA 3 cut(s) 853, 937, 967
Hpy8I GTNNAC 2 cut(s) 697, 1127
HpyAV CCTTC 3 cut(s) 247, 938, 1157
HpyCH4III ACNGT 5 cut(s) 499, 593, 598, 878, 1264
HpyCH4IV ACGT 1 cut(s) 909
HpyF10VI GCNNNNNNNGC 2 cut(s) 620, 791
HpyF3I CTNAG 7 cut(s) 156, 192, 330, 683, 738, 829, 966
HpySE526I ACGT 1 cut(s) 909
Hsp92II CATG 5 cut(s) 317, 677, 703, 991, 1122
Kzo9I GATC 7 cut(s) 148, 316, 679, 724, 853, 932, 1001
Lsp1109I GCAGC 4 cut(s) 331, 468, 632, 1137
LweI GCATC 2 cut(s) 111, 382
MaeI CTAG 2 cut(s) 789, 971
MaeII ACGT 1 cut(s) 909
MaeIII GTNAC 1 cut(s) 925
MalI GATC 7 cut(s) 150, 318, 681, 726, 855, 934, 1003
MboI GATC 7 cut(s) 148, 316, 679, 724, 853, 932, 1001
MfeI CAATTG 2 cut(s) 647, 711
MflI RGATCY 2 cut(s) 853, 1001
MluCI AATT 8 cut(s) 137, 300, 387, 647, 711, 814, 948, 1086
MlyI GAGTC 1 cut(s) 1115
MmeI TCCRAC 1 cut(s) 534
MnlI CCTC 5 cut(s) 135, 438, 594, 1149, 1232
MseI TTAA 9 cut(s) 339, 402, 477, 1026, 1085, 1092, 1155, 1257, 1270
MspA1I CMGCKG 1 cut(s) 1150
MspCI CTTAAG 2 cut(s) 1091, 1154
MspR9I CCNGG 2 cut(s) 517, 796
MunI CAATTG 2 cut(s) 647, 711
MvaI CCWGG 2 cut(s) 517, 796
MwoI GCNNNNNNNGC 2 cut(s) 620, 791
NdeII GATC 7 cut(s) 148, 316, 679, 724, 853, 932, 1001
NlaIII CATG 5 cut(s) 317, 677, 703, 991, 1122
NlaIV GGNNCC 2 cut(s) 786, 855
NspI RCATGY 2 cut(s) 703, 1122
PagI TCATGA 1 cut(s) 313
PaqCI CACCTGC 1 cut(s) 54
PfeI GAWTC 2 cut(s) 35, 484
PflFI GACNNNGTC 1 cut(s) 113
PkrI GCNGC 4 cut(s) 346, 458, 622, 1152
PleI GAGTC 1 cut(s) 1114
PpsI GAGTC 1 cut(s) 1114
Ppu21I YACGTR 1 cut(s) 910
PshBI ATTAAT 1 cut(s) 1026
Psp6I CCWGG 2 cut(s) 515, 794
PspFI CCCAGC 1 cut(s) 857
PspGI CCWGG 2 cut(s) 515, 794
PspN4I GGNNCC 2 cut(s) 786, 855
PspPI GGNCC 1 cut(s) 792
PstNI CAGNNNCTG 1 cut(s) 941
PsuI RGATCY 2 cut(s) 853, 1001
PsyI GACNNNGTC 1 cut(s) 113
PvuII CAGCTG 1 cut(s) 1150
RsaI GTAC 4 cut(s) 412, 595, 732, 1220
RsaNI GTAC 4 cut(s) 411, 594, 731, 1219
SaqAI TTAA 9 cut(s) 339, 402, 477, 1026, 1085, 1092, 1155, 1257, 1270
SatI GCNGC 4 cut(s) 345, 457, 621, 1151
Sau3AI GATC 7 cut(s) 148, 316, 679, 724, 853, 932, 1001
Sau96I GGNCC 1 cut(s) 792
SchI GAGTC 1 cut(s) 1115
ScrFI CCNGG 2 cut(s) 517, 796
SfaNI GCATC 2 cut(s) 111, 382
SmlI CTYRAG 6 cut(s) 126, 502, 528, 1062, 1091, 1154
SmoI CTYRAG 6 cut(s) 126, 502, 528, 1062, 1091, 1154
Sse9I AATT 8 cut(s) 137, 300, 387, 647, 711, 814, 948, 1086
SspMI CTAG 2 cut(s) 789, 971
StyD4I CCNGG 2 cut(s) 515, 794
StyI CCWWGG 2 cut(s) 788, 1018
TaaI ACNGT 5 cut(s) 499, 593, 598, 878, 1264
TaiI ACGT 1 cut(s) 912
TasI AATT 8 cut(s) 137, 300, 387, 647, 711, 814, 948, 1086
TatI WGTACW 1 cut(s) 730
TfiI GAWTC 2 cut(s) 35, 484
Tru1I TTAA 9 cut(s) 339, 402, 477, 1026, 1085, 1092, 1155, 1257, 1270
Tru9I TTAA 9 cut(s) 339, 402, 477, 1026, 1085, 1092, 1155, 1257, 1270
TscAI CASTG 1 cut(s) 883
TseI GCWGC 4 cut(s) 344, 456, 620, 1150
TspDTI ATGAA 8 cut(s) 27, 285, 302, 392, 396, 641, 827, 1004
TspRI CASTG 1 cut(s) 883
Tth111I GACNNNGTC 1 cut(s) 113
Vha464I CTTAAG 2 cut(s) 1091, 1154
VspI ATTAAT 1 cut(s) 1026
XceI RCATGY 2 cut(s) 703, 1122
XmaJI CCTAGG 1 cut(s) 788
XmiI GTMKAC 1 cut(s) 696
XspI CTAG 2 cut(s) 789, 971
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.