FvH4_5g24711
RLK Family

cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001471
Physical Location & Seq
Forward (+)
16067669 .. 16070422
2754 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g24711.t1

Sequence Viewer

Length: 1638 bp
ATGCCTAGCAAGACTCTTTCCATGCATTCTCCCTATGAACTTCTTCTTCATAAAATTCCAGATTTATCTCATCTTAGGATTTTTGGCTCTTGCTGCTATCCTTACCTTCGACCTTATGCTCATGACAAACTAGATCCTAGGACTACACCTTGTGTCTTCTTAGGGTATGCTTTAGGGTATAAAGGTGTGTTTTGCTACATCATATCTCAAAATAAACTTTGGATGTCAAGGCATGTTGTTCATGATGAGTCCATCTTTTCTTTTCATTCCTCTCCTCCTCCTGCCGCATCTCTTGTTGATCATACTTTCTTTCCCTCCTATTCTTTATTCACTGCTCTTCCTCCAAGGATGCCTGTCCCTTTGACTTCTACTGCTGGTCCTCCAACTAGTACTTCTGTTATCTTTCCATATGATTTTTCTGTTGGCAGAAGTGTTTTGAGCAACTTTTCTAACAGTACTATACATTCTTCTGATTCTGGTATTGATGTCCGAGATGTTGGTTCCGCAACAGTTCTTGAGGAGGATAGGAATGTAAGTCATGTTAATTTTGGGGTTAGCATGCAGGGAGGAAGTGTTGATGCTGTAGAGTCACATAGGCAACAGGACAGTCATGCAGTGGATTTGGAGAACCTGGTTGATCATGCAGTGGAAAATAATGTGGAAAATAATGAAGTAAATTTGGAGGATGGTGTTGAGTTGAACAGACAACAGGAAAATAATAATGGAGTCAGTTTGGAGGTTATTTTGCAGGATGATGATGAATTAAATAGTCATGCAATGGGAAGTCATGCAGTAGATTTGGAGAACCTGGTTGATCATGCAGTGGAAAATAATGCAGTAAATTTGGAGGATGGTGTTGAGTCAAATTCAGCTACTGATCAACAAGGTGTTGGCTCTCATAATGTTGTTAATGATCATGTTCAACATACAGATACTTCTGCTCTAACTATTAGTGACTTGAATGCTCTTGAATTTGGAGGTGTGGTAATATTGTTGTTGTATGTGGATGACATTGTCATCACTGGTTCGGATAAGGTGGGAATATCTGAGGTTATTTCTGAATTAAGCTTGAGGATATTGAGGTATTCACAAGGAACTGTAGCTAAAGGTCTTTTTTATAGCTCTTCTGGTGCCTTGACAAATGCAGTGCATATTAAAGCCTTCTGTGATGCTGACTGGGCTGGAGAGGTAATACAGAAACGATCCACTACTGGTTTTGTTGTGTATCTCGGTATGTGTCATGTTTCATGGCAATCAAAGAAGCAGGGAACTGTGTCTCGAAGTTCTACAGAGTCTGAATATCGCAGTCTTGCAAACACTGCTGCTGAAGTTAGCTGGATAAGGCATTTGCTGTGTGATTTGAAGATTCGGGTTCCTAGTCCTCCTATTCTTAAGTGTGATAATCTTTTAGCTCTTGCCTTAGCTTCTAATCCAGTTTTTCATTCAAAGATCAAGCATTTGGATAATGATTTCCATTTTGTTCGTGAACGTGCTCAACGCAATGATCTCCATCTTCAGTATGTTTCTACTACTGAACAAACAGCAGACATATTGACAAAGGGCTTGCCTTCTCTTTTTCGAACTCACTGTGTCAATCTCAGGCTAGTAACCCTAGCTGAGCTTGAGGGAGGCTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2 Retrotran_gag_3 Retrotrans_gag gag_pre-integrs rve

Protein Analysis

546

Amino Acids

59.94

Weight (kDa)

5.34

Isoelectric Point (pI)

42.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SH3_retrovirus PF25597 31 - 92 1.7e-16 Retroviral polymerase SH3-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1130
AciI CCGC 2 cut(s) 285, 504
AclWI GGATC 2 cut(s) 128, 1197
AcsI RAATTY 5 cut(s) 54, 676, 841, 865, 971
AcuI CTGAAG 2 cut(s) 1347, 1499
AdeI CACNNNGTG 1 cut(s) 152
AfaI GTAC 2 cut(s) 391, 457
AflII CTTAAG 1 cut(s) 1391
AgsI TTSAA 6 cut(s) 700, 923, 961, 971, 1363, 1446
AhlI ACTAGT 1 cut(s) 386
AjnI CCWGG 2 cut(s) 630, 807
AjuI GAANNNNNNNTTGG 2 cut(s) 376, 408
AluBI AGCT 9 cut(s) 872, 1068, 1103, 1122, 1335, 1412, 1424, 1616, 1621
AluI AGCT 9 cut(s) 872, 1068, 1103, 1122, 1335, 1412, 1424, 1616, 1621
Alw21I GWGCWC 1 cut(s) 1495
Alw26I GTCTC 1 cut(s) 1281
AlwI GGATC 2 cut(s) 128, 1197
AlwNI CAGNNNCTG 2 cut(s) 875, 1295
ApeKI GCWGC 2 cut(s) 93, 1322
ApoI RAATTY 5 cut(s) 54, 676, 841, 865, 971
Asp700I GAANNNNTTC 1 cut(s) 42
AspA2I CCTAGG 1 cut(s) 137
AspS9I GGNCC 1 cut(s) 377
AsuII TTCGAA 1 cut(s) 1579
AvaII GGWCC 1 cut(s) 377
AvrII CCTAGG 1 cut(s) 137
BanI GGYRCC 1 cut(s) 1130
BbsI GAAGAC 1 cut(s) 148
Bbv12I GWGCWC 1 cut(s) 1495
BbvI GCAGC 2 cut(s) 80, 1309
BccI CCATC 4 cut(s) 260, 680, 845, 1518
BciT130I CCWGG 2 cut(s) 632, 809
BclI TGATCA 5 cut(s) 298, 637, 814, 877, 913
BcoDI GTCTC 1 cut(s) 1281
BcuI ACTAGT 1 cut(s) 386
BfaI CTAG 7 cut(s) 6, 131, 138, 387, 1377, 1604, 1613
BfmI CTRYAG 3 cut(s) 582, 1098, 1287
BfrI CTTAAG 1 cut(s) 1391
BisI GCNGC 3 cut(s) 94, 285, 1323
BlnI CCTAGG 1 cut(s) 137
BlpI GCTNAGC 1 cut(s) 1617
BlsI GCNGC 3 cut(s) 95, 286, 1324
BmcAI AGTACT 2 cut(s) 391, 457
Bme1390I CCNGG 2 cut(s) 632, 809
Bme18I GGWCC 1 cut(s) 377
BmgT120I GGNCC 1 cut(s) 377
BmiI GGNNCC 3 cut(s) 502, 1132, 1374
BmrFI CCNGG 2 cut(s) 632, 809
BmrI ACTGGG 1 cut(s) 1186
BmsI GCATC 4 cut(s) 296, 339, 568, 1159
BmuI ACTGGG 1 cut(s) 1186
BpiI GAAGAC 1 cut(s) 148
BpmI CTGGAG 1 cut(s) 1203
Bpu10I CCTNAGC 1 cut(s) 1420
Bpu1102I GCTNAGC 1 cut(s) 1617
Bpu14I TTCGAA 1 cut(s) 1579
BpuEI CTTGAG 2 cut(s) 536, 1090
BsaBI GATNNNNATC 1 cut(s) 1509
BsaJI CCNNGG 2 cut(s) 137, 344
BsaXI ACNNNNNCTCC 4 cut(s) 558, 588, 969, 999
Bse1I ACTGG 4 cut(s) 1027, 1181, 1216, 1433
Bse3DI GCAATG 2 cut(s) 783, 1507
Bse8I GATNNNNATC 1 cut(s) 1509
BseBI CCWGG 2 cut(s) 632, 809
BseDI CCNNGG 2 cut(s) 137, 344
BseGI GGATG 6 cut(s) 228, 354, 691, 757, 856, 1012
BseJI GATNNNNATC 1 cut(s) 1509
BseMI GCAATG 2 cut(s) 783, 1507
BseMII CTCAG 3 cut(s) 1038, 1608, 1612
BseNI ACTGG 4 cut(s) 1027, 1181, 1216, 1433
BseRI GAGGAG 3 cut(s) 264, 267, 533
BseXI GCAGC 2 cut(s) 80, 1309
BshNI GGYRCC 1 cut(s) 1130
BsiHKAI GWGCWC 1 cut(s) 1495
BslFI GGGAC 1 cut(s) 341
BsmAI GTCTC 1 cut(s) 1281
BsmFI GGGAC 1 cut(s) 341
BsmI GAATGC 2 cut(s) 25, 967
Bsp119I TTCGAA 1 cut(s) 1579
Bsp1286I GDGCHC 1 cut(s) 1495
Bsp143I GATC 9 cut(s) 133, 298, 637, 814, 877, 913, 1202, 1449, 1504
Bsp1720I GCTNAGC 1 cut(s) 1617
BspACI CCGC 2 cut(s) 285, 504
BspCNI CTCAG 3 cut(s) 1039, 1609, 1611
BspHI TCATGA 2 cut(s) 121, 241
BspLI GGNNCC 3 cut(s) 502, 1132, 1374
BspPI GGATC 2 cut(s) 128, 1197
BspQI GCTCTTC 2 cut(s) 342, 1129
BspT104I TTCGAA 1 cut(s) 1579
BspT107I GGYRCC 1 cut(s) 1130
BspTI CTTAAG 1 cut(s) 1391
BsrDI GCAATG 2 cut(s) 783, 1507
BsrI ACTGG 4 cut(s) 1027, 1181, 1216, 1433
BssECI CCNNGG 2 cut(s) 137, 344
BssMI GATC 9 cut(s) 133, 298, 637, 814, 877, 913, 1202, 1449, 1504
BssT1I CCWWGG 2 cut(s) 137, 344
Bst2UI CCWGG 2 cut(s) 632, 809
Bst4CI ACNGT 6 cut(s) 455, 511, 608, 1099, 1273, 1589
Bst6I CTCTTC 2 cut(s) 342, 1129
BstAFI CTTAAG 1 cut(s) 1391
BstAPI GCANNNNNTGC 1 cut(s) 1319
BstBI TTCGAA 1 cut(s) 1579
BstC8I GCNNGC 2 cut(s) 560, 1565
BstDEI CTNAG 6 cut(s) 74, 160, 1047, 1420, 1598, 1617
BstF5I GGATG 6 cut(s) 228, 354, 691, 757, 856, 1012
BstKTI GATC 9 cut(s) 136, 301, 640, 817, 880, 916, 1205, 1452, 1507
BstMAI GTCTC 1 cut(s) 1281
BstMBI GATC 9 cut(s) 133, 298, 637, 814, 877, 913, 1202, 1449, 1504
BstMWI GCNNNNNNNGC 3 cut(s) 93, 1178, 1319
BstNI CCWGG 2 cut(s) 632, 809
BstNSI RCATGY 2 cut(s) 236, 562
BstSCI CCNGG 2 cut(s) 630, 807
BstSFI CTRYAG 3 cut(s) 582, 1098, 1287
BstV1I GCAGC 2 cut(s) 80, 1309
BstV2I GAAGAC 1 cut(s) 148
BstX2I RGATCY 1 cut(s) 133
BstYI RGATCY 1 cut(s) 133
BtsCI GGATG 6 cut(s) 228, 354, 691, 757, 856, 1012
BtsI GCAGTG 6 cut(s) 330, 621, 651, 828, 1152, 1317
BtsIMutI CAGTG 8 cut(s) 330, 621, 651, 828, 1020, 1152, 1317, 1585
Cac8I GCNNGC 2 cut(s) 560, 1565
CaiI CAGNNNCTG 2 cut(s) 875, 1295
CciI TCATGA 2 cut(s) 121, 241
Cfr13I GGNCC 1 cut(s) 377
CsiI ACCWGGT 2 cut(s) 630, 807
Csp6I GTAC 2 cut(s) 390, 456
CviQI GTAC 2 cut(s) 390, 456
DdeI CTNAG 6 cut(s) 74, 160, 1047, 1420, 1598, 1617
DpnI GATC 9 cut(s) 135, 300, 639, 816, 879, 915, 1204, 1451, 1506
DpnII GATC 9 cut(s) 133, 298, 637, 814, 877, 913, 1202, 1449, 1504
DraIII CACNNNGTG 1 cut(s) 152
Eam1104I CTCTTC 2 cut(s) 342, 1129
EarI CTCTTC 2 cut(s) 342, 1129
Eco130I CCWWGG 2 cut(s) 137, 344
Eco47I GGWCC 1 cut(s) 377
Eco57I CTGAAG 2 cut(s) 1347, 1499
EcoRII CCWGG 2 cut(s) 630, 807
EcoT14I CCWWGG 2 cut(s) 137, 344
EcoT22I ATGCAT 1 cut(s) 27
ErhI CCWWGG 2 cut(s) 137, 344
FaqI GGGAC 1 cut(s) 341
FauNDI CATATG 1 cut(s) 409
FbaI TGATCA 5 cut(s) 298, 637, 814, 877, 913
Fnu4HI GCNGC 3 cut(s) 94, 285, 1323
FokI GGATG 6 cut(s) 235, 361, 698, 764, 863, 1019
Fsp4HI GCNGC 3 cut(s) 94, 285, 1323
FspBI CTAG 7 cut(s) 6, 131, 138, 387, 1377, 1604, 1613
GluI GCNGC 3 cut(s) 94, 285, 1323
GsuI CTGGAG 1 cut(s) 1203
HindIII AAGCTT 1 cut(s) 1066
HinfI GANTC 8 cut(s) 13, 248, 473, 587, 726, 860, 1292, 1366
Hpy166II GTNNAC 1 cut(s) 1487
Hpy188I TCNGA 6 cut(s) 472, 491, 1030, 1048, 1060, 1297
Hpy188III TCNNGA 7 cut(s) 59, 122, 242, 515, 968, 1278, 1484
Hpy8I GTNNAC 1 cut(s) 1487
HpyAV CCTTC 3 cut(s) 116, 1171, 1578
HpyCH4III ACNGT 6 cut(s) 455, 511, 608, 1099, 1273, 1589
HpyCH4IV ACGT 1 cut(s) 1489
HpyF10VI GCNNNNNNNGC 3 cut(s) 93, 1178, 1319
HpyF3I CTNAG 6 cut(s) 74, 160, 1047, 1420, 1598, 1617
HpySE526I ACGT 1 cut(s) 1489
Ksp22I TGATCA 5 cut(s) 298, 637, 814, 877, 913
Kzo9I GATC 9 cut(s) 133, 298, 637, 814, 877, 913, 1202, 1449, 1504
LguI GCTCTTC 2 cut(s) 342, 1129
Lsp1109I GCAGC 2 cut(s) 80, 1309
LweI GCATC 4 cut(s) 296, 339, 568, 1159
MabI ACCWGGT 2 cut(s) 630, 807
MaeI CTAG 7 cut(s) 6, 131, 138, 387, 1377, 1604, 1613
MaeII ACGT 1 cut(s) 1489
MaeIII GTNAC 3 cut(s) 588, 953, 1606
MalI GATC 9 cut(s) 135, 300, 639, 816, 879, 915, 1204, 1451, 1506
MboI GATC 9 cut(s) 133, 298, 637, 814, 877, 913, 1202, 1449, 1504
MboII GAAGA 8 cut(s) 35, 38, 148, 329, 459, 1116, 1375, 1505
MflI RGATCY 1 cut(s) 133
MhlI GDGCHC 1 cut(s) 1495
MluCI AATT 8 cut(s) 54, 544, 676, 761, 841, 865, 971, 1061
MlyI GAGTC 6 cut(s) 7, 257, 596, 735, 869, 1301
MmeI TCCRAC 1 cut(s) 407
Mph1103I ATGCAT 1 cut(s) 27
MroXI GAANNNNTTC 1 cut(s) 42
MseI TTAA 6 cut(s) 543, 764, 909, 1064, 1155, 1392
MspCI CTTAAG 1 cut(s) 1391
MspR9I CCNGG 2 cut(s) 632, 809
Mva1269I GAATGC 2 cut(s) 25, 967
MvaI CCWGG 2 cut(s) 632, 809
MwoI GCNNNNNNNGC 3 cut(s) 93, 1178, 1319
NdeI CATATG 1 cut(s) 409
NdeII GATC 9 cut(s) 133, 298, 637, 814, 877, 913, 1202, 1449, 1504
NlaIV GGNNCC 3 cut(s) 502, 1132, 1374
NmuCI GTSAC 2 cut(s) 588, 953
NsiI ATGCAT 1 cut(s) 27
NspI RCATGY 2 cut(s) 236, 562
NspV TTCGAA 1 cut(s) 1579
PaeI GCATGC 1 cut(s) 562
PagI TCATGA 2 cut(s) 121, 241
PciSI GCTCTTC 2 cut(s) 342, 1129
PctI GAATGC 2 cut(s) 25, 967
PdmI GAANNNNTTC 1 cut(s) 42
PfeI GAWTC 2 cut(s) 473, 1366
PflFI GACNNNGTC 1 cut(s) 1013
PkrI GCNGC 3 cut(s) 95, 286, 1324
PleI GAGTC 6 cut(s) 7, 256, 595, 734, 868, 1300
PpsI GAGTC 6 cut(s) 7, 256, 595, 734, 868, 1300
Psp6I CCWGG 2 cut(s) 630, 807
PspGI CCWGG 2 cut(s) 630, 807
PspN4I GGNNCC 3 cut(s) 502, 1132, 1374
PspPI GGNCC 1 cut(s) 377
PstNI CAGNNNCTG 2 cut(s) 875, 1295
PsuI RGATCY 1 cut(s) 133
PsyI GACNNNGTC 1 cut(s) 1013
RsaI GTAC 2 cut(s) 391, 457
RsaNI GTAC 2 cut(s) 390, 456
SapI GCTCTTC 2 cut(s) 342, 1129
SaqAI TTAA 6 cut(s) 543, 764, 909, 1064, 1155, 1392
SatI GCNGC 3 cut(s) 94, 285, 1323
Sau3AI GATC 9 cut(s) 133, 298, 637, 814, 877, 913, 1202, 1449, 1504
Sau96I GGNCC 1 cut(s) 377
ScaI AGTACT 2 cut(s) 391, 457
SchI GAGTC 6 cut(s) 7, 257, 596, 735, 869, 1301
ScrFI CCNGG 2 cut(s) 632, 809
SduI GDGCHC 1 cut(s) 1495
SexAI ACCWGGT 2 cut(s) 630, 807
SfaNI GCATC 4 cut(s) 296, 339, 568, 1159
SfcI CTRYAG 3 cut(s) 582, 1098, 1287
SfuI TTCGAA 1 cut(s) 1579
SinI GGWCC 1 cut(s) 377
SmlI CTYRAG 4 cut(s) 515, 1069, 1391, 1622
SmoI CTYRAG 4 cut(s) 515, 1069, 1391, 1622
SpeI ACTAGT 1 cut(s) 386
SphI GCATGC 1 cut(s) 562
Sse9I AATT 8 cut(s) 54, 544, 676, 761, 841, 865, 971, 1061
SsiI CCGC 2 cut(s) 285, 504
SspI AATATT 1 cut(s) 990
SspMI CTAG 7 cut(s) 6, 131, 138, 387, 1377, 1604, 1613
StyD4I CCNGG 2 cut(s) 630, 807
StyI CCWWGG 2 cut(s) 137, 344
TaaI ACNGT 6 cut(s) 455, 511, 608, 1099, 1273, 1589
TaiI ACGT 1 cut(s) 1492
TaqI TCGA 3 cut(s) 109, 1279, 1579
TasI AATT 8 cut(s) 54, 544, 676, 761, 841, 865, 971, 1061
TatI WGTACW 2 cut(s) 389, 455
TauI GCSGC 1 cut(s) 287
TfiI GAWTC 2 cut(s) 473, 1366
Tru1I TTAA 6 cut(s) 543, 764, 909, 1064, 1155, 1392
Tru9I TTAA 6 cut(s) 543, 764, 909, 1064, 1155, 1392
TscAI CASTG 8 cut(s) 337, 621, 651, 828, 1027, 1152, 1324, 1592
TseFI GTSAC 2 cut(s) 588, 953
TseI GCWGC 2 cut(s) 93, 1322
Tsp45I GTSAC 2 cut(s) 588, 953
TspDTI ATGAA 8 cut(s) 38, 51, 230, 254, 684, 774, 1236, 1430
TspRI CASTG 8 cut(s) 337, 621, 651, 828, 1027, 1152, 1324, 1592
Tth111I GACNNNGTC 1 cut(s) 1013
Vha464I CTTAAG 1 cut(s) 1391
VpaK11BI GGWCC 1 cut(s) 377
XapI RAATTY 5 cut(s) 54, 676, 841, 865, 971
XceI RCATGY 2 cut(s) 236, 562
XmaJI CCTAGG 1 cut(s) 137
XmnI GAANNNNTTC 1 cut(s) 42
XspI CTAG 7 cut(s) 6, 131, 138, 387, 1377, 1604, 1613
ZrmI AGTACT 2 cut(s) 391, 457
Zsp2I ATGCAT 1 cut(s) 27
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.