RchiOBHm_Chr2g0141981

transposition

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0001471
Physical Location & Seq
Reverse (-)
59518282 .. 59524063
5782 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ51221

Sequence Viewer

Length: 3993 bp
ATGGCGCCTACTGCTATGGCTATGTCACAATCTGAGATCAACTCTTTGCTAAGCACAATAACTGTTCGGTTGAGTGAAAACAATTTTGTGAAGTGGAGCTTTCAGTTTCAAGCAGTTCTTGAAGGCAATGATTTGTTTGGATATTTTGATGGCTCATATCCCTGTCCTCCTCGGTTTGTTCTCACTGAGGAAGGTCAAGTCACTAGTGAGATTACTATGGCCTATAAACTGTGGAAGAAAACAGATAAAGCCTTGCTTGGTCTCCTCATGGCCACTCTTGATGGTGATATCATGGATATTATAGTAGGAAGTAAATCTTCTCATGAGGCCTGGCTTGCATTACTCGAAAGGTTCTCAACAGTCTCTCGAGCTAATATCATTCAGCTTAAAACTGATCTACAAACTATCAAGAAGGGAGCAGATACCATTGACAAGTATCTTCAACGCATCAAGCATGCTAGAGATCAATTAAGCTCTGTGGGAGTAACTCTTGTTGATGAGGATATTGTGGTGGTTACATTGAATGGCTTACCAGATGAATATGCAATGATAAAAACTGTCATCAGGGCAAGAGATACTCCTATCTCACTGAAAGACTTTAGAGCTCAATTATTAGTTGCTGAGAGAGATATTGAGTCACAGCTTGTACCTCATACATCTATGTCTGCTATGGCTGCAAAGAGCTCATCCTACAGAGGTAATAACAGCTCACAGTTCTCTGGATCATCAAGTACCTATAATGTGGACAAGGGAAAAGCAGTGTGGAATGACAATTCTAAGTCTGAAACCTGGAATGGCAATGGAAAGTATTCTCAAAGCAAAAACTCTAGTGGTGGGGGATTCAGGAACTACAATGGAGTGGAATGTCAAGTATGTGGTAAGAAAGGACATATGGCAAGCAATTGCTATCAGAACATGGAATGTCACATTTGCCACAAGAAAGGTCACATTGCTAGCAAGTGCTTTCAGAATCCTGCTAATAATAATCAAGGGGTTGAATATAGAAACAATATTGGTGCTTCTCCTGAGTGCCAAATCTGCAGTAAGAAGGGACACACAGCTATTAATTGTTTCTATAGAGGTGACATACCCCTTGATCATCCTTCTAGGTCAGTGGTGGTTTGTCAAATCTGCGGATTGAAAGGCCGTGTTGCACTCAACTGCCATCATAGAAGCAACTATGCCTATCAGGGATCTGATCCTCCAAACTCTCTCACCGCCATGACAGCCCGCACTAATTGTTCTGGTGCAAGTTCTTCAAGTTCTTCTAACAGCAACAACACTGAAGTGTGGATTGGTGATACTGGAGCTACTCACCATATGACATCTGATCTGAGAAATCTTTCCATTGCTCATCCTTTTGACTCCAATAACACCATTACAATTGGTAATGGAGAAGGTTTGCATGACAAGGCATCAAAAGCTCTTCTTTACAGAGGAAAGAGTAATGGGGAAGGGCTATTTCTCTTCAAAACACCAAAATCCATACCTCCATTTCATCATGATCATACAGCTTTTGTTGGAGCTGCTGTCAAATGCTCTCTCTGGCACCAAAGGCTTGGTCATCCCTCCTCAGATATAGTGTCTAGGATGTTGTCTTTGTCTCAGGTCAAGTTTAATAATGATCAAACTTCTCATACTTGTTCTCATTGCCTTAGTGGCAAGATGCATAGATTGCCATTTTCTGAGTCTATCTCAAAATCTGTACTTCCATTTCAAAAGGTACATTCTGACCTTTGGGGTCCTGCTCCTTGTTTGTCCATTGAGGGTTTCAAATACTATGTAACATTCATCGATGATTATACCAAATTTGTGTGGGTTTATCCACTCATCAATAAATCTGATACTTTTTCCACATTTGTCAAGTTTCACAACTTTGTTTCCACTCAGTTCCGGCATAATATTCTCTCTCTCCAAACTGATGGTGGTGGTGAATATAATAGCAAAGCTTTTAGAAATTATCTTGATCACAAAGGCATTGTGCATTTTATCACTTGTCCTCACACTCCCCAACAAAATGGAGTGGCAGAAAGAAAGAATCGTCATATTGTTGAGACTTGTATCACTCTCATGTCTGCAGCTAAGATGCCTAAACCTTTTTGGTTTCATGCTGTTGCTCATTCTGCATATCTCATAAATAGGATGCCTTGCAAATCTTTGAATATGTCATCTCCTTATATCCAGTTCTTTGGTTCTGCACCTGATCTTAGTCATCTTAAAATCTTTGGTTCAGCATGCTATCCCTTTCTTCGCCCTTACTGTCATGATAAACTTGATCCAAGGACTACCAAGTGTGTATTTTTGGGGTATGCTCTAGGGTATAAGGGTGTGTTTTGCTACAGCATTGAGAAAAACAAGCTTTGGATGTGCAGACATGTGATTCATGATGAGTCAATTTTTCCTTTTCAATCTTCTGGCAGTGACAACTCATCTAAGCCTACACATGTATCCTCTTCTGCTTCTGCATCTTCTCCTCATTTTTTTCCACCTATGATGCCTGCTCTTTCTTCTGATCAGATGCAAGCGGTGCTTCCTTTTGTTAACACTCTTCCGCCTGCTGCTAGTCTCCCTGCATTGGCTGATGGGGGGGCTGATGGTTCTGTTGCACATGCAGATTTTGTACCTCATGATCATGATGATCATGAAAATTCTGAAAATTCTGAAAATGTTGAAAATAATGTTGGTGAGGCAAGTGGAAATGATAGTGATACCAGTTTTGGTGGTGTTAGTCCTGAAGGTCAAGTCTTGTCACAGGAGTTACAATTTCTTGATGATCCTGCCCTTGCATATAATAATCACTCCATGTTAACTAGAGCTAAGAATGGAATATTCAAGAAGAAGTCATTTGATGAGTTTTGTGCTTATTCCTGTGTTGTTCAGAACAATCTACTTGATGAACTCTCTTGTTTTAGTGGTCTTACAGCTGTAATTGATATTCATGATCCTGTTGAGCCAAAGAGTTTTAAAGCTGCAGTTGGTGTACCAGAGTGGGATATTGCTATGAATGAAGAAATTGATGCATTGAAAAAGCAAGCTACATGGGAATTGGTTCCCTTTCCAGAAGGTAAGAATATTGTTGGAAGCAAATGGGTGTATAGAATTAAGAAAAATCCTGATGGAAGTGTTTCTAGGTTTAAAGCAAGGTTAGTTGCTCAAGGATATAGTCAAGAGAAAGGTTTGGACTATGATGAAACTTTCAGTCCTGTGGTGAGACATAGCACAGTAAGAATTATTCTTTCTCTAGCTGCAATGAACAATTGGGAATTAAGGCAACTTGATGTTAAGAATGCTTTTTTACATGGTGATCTTAAAGAAGAAGTATATATGCATCAGCCTCTCGGATTTGTTGATTCTGTGCATCCCAATTATGTCTGCTTACTCAGGAAATATCTTTATGGATTAAAGCAAGCACCTAGAGCTTGGAATGAAAAATTCACTAATTTTCTTCCTGCCTTGGGATTTAAATTCTCTCATTCTGACCCAAGCTTGTTTGTTAAGGCTACAAGTGATGGGATAATTGCATTGTTGCTTTATGTTGATGACATAGTGATTACTGGATCAGATAAGAATGGTGTGTCATCTGTAATTTCTGAACTCAGTGATGTCTTTGATATGAAAGACCTTGGGCCTCTATCTTACTTTCTGGGAATAAATGTTCAATACAAAGACAAAGGACTTTTCTTGTCACAGGAAACATATGCCAAGGACTTGATTGCCAAGGCTGGGTTAGATACTTGCAGAGAGTGTAATACTCCATGCTTGCCACATGCTCAACTGCTCAAAAATGAAGGTACACCTTTGTCAAATCCCTCTCTTTACAGAAGCATTGTTGGTGCTTTACAATACTTGACATTTACTAGGCCAGACATTGCCTTTGCTGTAAACACAGTGTGTCAATTTATGTCTCAACCAACTGATGTGCATTATGCTTCTGTTAAGAGAATTTTGAGATATATATCTTCAAGGTACCATATCAAAAGGACTGTTTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HTH_21 rve

Protein Analysis

1330

Amino Acids

147.67

Weight (kDa)

7.92

Isoelectric Point (pI)

39.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotran_gag_2 PF14223 37 - 209 4.7e-23 gag-polypeptide of LTR copia-type
gag_pre-integrs PF13976 498 - 556 4e-10 GAG-pre-integrase domain
rve PF00665 571 - 670 1.2e-11 Integrase core domain
SH3_retrovirus PF25597 744 - 805 1.8e-15 Retroviral polymerase SH3-like domain
RVT_2 PF07727 1012 - 1254 5.2e-73 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3991
Acc65I GGTACC 1 cut(s) 3966
AccB1I GGYRCC 3 cut(s) 4, 1548, 3966
AciI CCGC 5 cut(s) 1134, 1218, 1231, 2525, 2552
AclWI GGATC 7 cut(s) 730, 1193, 1201, 2270, 2768, 2936, 3565
AcoI YGGCCR 1 cut(s) 270
AcsI RAATTY 6 cut(s) 1808, 2647, 2656, 3431, 3464, 3942
AcuI CTGAAG 2 cut(s) 1305, 2754
AcyI GRCGYC 1 cut(s) 5
AdeI CACNNNGTG 1 cut(s) 3891
AfaI GTAC 8 cut(s) 648, 733, 1707, 1725, 2622, 2982, 3793, 3968
AfiI CCNNNNNNNGG 3 cut(s) 2575, 3455, 3723
AflIII ACRYGT 2 cut(s) 2374, 2443
AhlI ACTAGT 1 cut(s) 203
AjnI CCWGG 2 cut(s) 329, 788
AjuI GAANNNNNNNTTGG 4 cut(s) 1278, 1310, 2664, 2696
AleI CACNNNNGTG 1 cut(s) 1286
AloI GAACNNNNNNTCC 2 cut(s) 3639, 3671
Alw21I GWGCWC 2 cut(s) 607, 686
Alw26I GTCTC 7 cut(s) 266, 367, 1608, 2048, 2570, 3205, 3909
AlwI GGATC 7 cut(s) 730, 1193, 1201, 2270, 2768, 2936, 3565
Ama87I CYCGRG 1 cut(s) 366
AoxI GGCC 6 cut(s) 219, 270, 327, 1144, 3626, 3860
ApeKI GCWGC 6 cut(s) 674, 1526, 2078, 2558, 2969, 3245
ApoI RAATTY 6 cut(s) 1808, 2647, 2656, 3431, 3464, 3942
ArsI GACNNNNNNTTYG 4 cut(s) 1546, 1578, 3857, 3889
AseI ATTAAT 1 cut(s) 1065
Asp700I GAANNNNTTC 4 cut(s) 808, 1342, 3048, 3124
Asp718I GGTACC 1 cut(s) 3966
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 2 cut(s) 1742, 3626
AsuHPI GGTGA 9 cut(s) 296, 1094, 1207, 1307, 1310, 1943, 2696, 3220, 3314
AsuNHI GCTAGC 1 cut(s) 953
AvaI CYCGRG 1 cut(s) 366
AvaII GGWCC 1 cut(s) 1742
BaeI ACNNNNGTAYC 2 cut(s) 2430, 2463
BalI TGGCCA 1 cut(s) 272
BanI GGYRCC 3 cut(s) 4, 1548, 3966
BanII GRGCYC 2 cut(s) 607, 686
Bbv12I GWGCWC 2 cut(s) 607, 686
BbvI GCAGC 6 cut(s) 661, 1513, 2090, 2545, 2956, 3232
BccI CCATC 8 cut(s) 143, 275, 1173, 1916, 2576, 2588, 3110, 3503
BceAI ACGGC 1 cut(s) 1131
BciT130I CCWGG 2 cut(s) 331, 790
BciVI GTATCC 1 cut(s) 2458
BclI TGATCA 7 cut(s) 1096, 1504, 1624, 1966, 2512, 2629, 2638
BcoDI GTCTC 7 cut(s) 266, 367, 1608, 2048, 2570, 3205, 3909
BcuI ACTAGT 1 cut(s) 203
BfmI CTRYAG 6 cut(s) 691, 1039, 1075, 2076, 2338, 2970
BfoI RGCGCY 1 cut(s) 8
BfuI GTATCC 1 cut(s) 2458
BglI GCCNNNNNGGC 1 cut(s) 1659
BisI GCNGC 6 cut(s) 675, 1527, 2079, 2559, 2970, 3246
BlpI GCTNAGC 1 cut(s) 50
BlsI GCNGC 6 cut(s) 676, 1528, 2080, 2560, 2971, 3247
Bme1390I CCNGG 2 cut(s) 331, 790
Bme18I GGWCC 1 cut(s) 1742
BmeT110I CYCGRG 1 cut(s) 366
BmgT120I GGNCC 2 cut(s) 1742, 3626
BmiI GGNNCC 5 cut(s) 6, 1550, 1743, 3051, 3968
BmrFI CCNGG 2 cut(s) 331, 790
BmtI GCTAGC 1 cut(s) 957
BplI GAGNNNNNCTC 4 cut(s) 26, 58, 1679, 1711
BpmI CTGGAG 1 cut(s) 1326
Bpu1102I GCTNAGC 1 cut(s) 50
BpuEI CTTGAG 1 cut(s) 3138
Bsa29I ATCGAT 1 cut(s) 1794
BsaBI GATNNNNATC 1 cut(s) 3955
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 266
BsaJI CCNNGG 6 cut(s) 170, 2279, 3453, 3622, 3702, 3717
BsaXI ACNNNNNCTCC 2 cut(s) 1515, 1545
Bsc4I CCNNNNNNNGG 3 cut(s) 2575, 3455, 3723
Bse1I ACTGG 4 cut(s) 1309, 2182, 2712, 3559
Bse3DI GCAATG 8 cut(s) 133, 552, 805, 948, 1347, 1648, 3255, 3867
Bse8I GATNNNNATC 1 cut(s) 3955
BseBI CCWGG 2 cut(s) 331, 790
BseCI ATCGAT 1 cut(s) 1794
BseDI CCNNGG 6 cut(s) 170, 2279, 3453, 3622, 3702, 3717
BseGI GGATG 8 cut(s) 686, 1099, 1354, 1564, 1596, 2148, 2370, 3358
BseJI GATNNNNATC 1 cut(s) 3955
BseLI CCNNNNNNNGG 3 cut(s) 2575, 3455, 3723
BseMI GCAATG 8 cut(s) 133, 552, 805, 948, 1347, 1648, 3255, 3867
BseNI ACTGG 4 cut(s) 1309, 2182, 2712, 3559
BseRI GAGGAG 4 cut(s) 159, 254, 1561, 2463
BseXI GCAGC 6 cut(s) 661, 1513, 2090, 2545, 2956, 3232
BseYI CCCAGC 1 cut(s) 3722
BsgI GTGCAG 2 cut(s) 2181, 2389
BshFI GGCC 6 cut(s) 221, 272, 329, 1146, 3628, 3862
BshNI GGYRCC 3 cut(s) 4, 1548, 3966
BshVI ATCGAT 1 cut(s) 1794
BsiHKAI GWGCWC 2 cut(s) 607, 686
BsiHKCI CYCGRG 1 cut(s) 366
BsiSI CCGG 1 cut(s) 1894
BslFI GGGAC 1 cut(s) 1065
BslI CCNNNNNNNGG 3 cut(s) 2575, 3455, 3723
BsmAI GTCTC 7 cut(s) 266, 367, 1608, 2048, 2570, 3205, 3909
BsmFI GGGAC 1 cut(s) 1065
BsmI GAATGC 1 cut(s) 3292
BsnI GGCC 6 cut(s) 221, 272, 329, 1146, 3628, 3862
Bso31I GGTCTC 1 cut(s) 266
BsoBI CYCGRG 1 cut(s) 366
Bsp1286I GDGCHC 2 cut(s) 607, 686
Bsp1720I GCTNAGC 1 cut(s) 50
BspACI CCGC 5 cut(s) 1134, 1218, 1231, 2525, 2552
BspANI GGCC 6 cut(s) 221, 272, 329, 1146, 3628, 3862
BspDI ATCGAT 1 cut(s) 1794
BspHI TCATGA 8 cut(s) 322, 1501, 2263, 2383, 2626, 2632, 2641, 2938
BspLI GGNNCC 5 cut(s) 6, 1550, 1743, 3051, 3968
BspMAI CTGCAG 3 cut(s) 1043, 2080, 2974
BspOI GCTAGC 1 cut(s) 957
BspPI GGATC 7 cut(s) 730, 1193, 1201, 2270, 2768, 2936, 3565
BspQI GCTCTTC 1 cut(s) 1431
BspT107I GGYRCC 3 cut(s) 4, 1548, 3966
BspTNI GGTCTC 1 cut(s) 266
BsrDI GCAATG 8 cut(s) 133, 552, 805, 948, 1347, 1648, 3255, 3867
BsrI ACTGG 4 cut(s) 1309, 2182, 2712, 3559
BssECI CCNNGG 6 cut(s) 170, 2279, 3453, 3622, 3702, 3717
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 5 cut(s) 2279, 3453, 3622, 3702, 3717
Bst2UI CCWGG 2 cut(s) 331, 790
Bst4CI ACNGT 9 cut(s) 64, 231, 361, 559, 714, 2261, 3223, 3889, 3985
Bst6I CTCTTC 4 cut(s) 1431, 1472, 2458, 2553
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 2 cut(s) 1675, 2527
BstF5I GGATG 8 cut(s) 686, 1099, 1354, 1564, 1596, 2148, 2370, 3358
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstMAI GTCTC 7 cut(s) 266, 367, 1608, 2048, 2570, 3205, 3909
BstNI CCWGG 2 cut(s) 331, 790
BstNSI RCATGY 6 cut(s) 458, 2238, 2378, 2447, 2612, 3770
BstSCI CCNGG 2 cut(s) 329, 788
BstSFI CTRYAG 6 cut(s) 691, 1039, 1075, 2076, 2338, 2970
BstV1I GCAGC 6 cut(s) 661, 1513, 2090, 2545, 2956, 3232
BstX2I RGATCY 1 cut(s) 1193
BstXI CCANNNNNNTGG 4 cut(s) 1559, 1922, 2018, 2189
BstYI RGATCY 1 cut(s) 1193
Bsu15I ATCGAT 1 cut(s) 1794
BsuI GTATCC 1 cut(s) 2458
BsuRI GGCC 6 cut(s) 221, 272, 329, 1146, 3628, 3862
BsuTUI ATCGAT 1 cut(s) 1794
BtsCI GGATG 8 cut(s) 686, 1099, 1354, 1564, 1596, 2148, 2370, 3358
BtsI GCAGTG 2 cut(s) 765, 2425
BtsIMutI CAGTG 8 cut(s) 183, 587, 765, 1119, 1281, 2425, 3604, 3894
CciI TCATGA 8 cut(s) 322, 1501, 2263, 2383, 2626, 2632, 2641, 2938
CfoI GCGC 1 cut(s) 7
Cfr13I GGNCC 2 cut(s) 1742, 3626
ClaI ATCGAT 1 cut(s) 1794
Csp6I GTAC 8 cut(s) 647, 732, 1706, 1724, 2621, 2981, 3792, 3967
CviQI GTAC 8 cut(s) 647, 732, 1706, 1724, 2621, 2981, 3792, 3967
DinI GGCGCC 1 cut(s) 6
DraI TTTAAA 3 cut(s) 2965, 3136, 3463
DraIII CACNNNGTG 1 cut(s) 3891
EaeI YGGCCR 1 cut(s) 270
Eam1104I CTCTTC 4 cut(s) 1431, 1472, 2458, 2553
EarI CTCTTC 4 cut(s) 1431, 1472, 2458, 2553
EciI GGCGGA 1 cut(s) 2541
Ecl136II GAGCTC 2 cut(s) 605, 684
Eco130I CCWWGG 5 cut(s) 2279, 3453, 3622, 3702, 3717
Eco147I AGGCCT 1 cut(s) 329
Eco24I GRGCYC 2 cut(s) 607, 686
Eco31I GGTCTC 1 cut(s) 266
Eco32I GATATC 1 cut(s) 289
Eco47I GGWCC 1 cut(s) 1742
Eco53kI GAGCTC 2 cut(s) 605, 684
Eco57I CTGAAG 2 cut(s) 1305, 2754
Eco88I CYCGRG 1 cut(s) 366
EcoICRI GAGCTC 2 cut(s) 605, 684
EcoO109I RGGNCCY 1 cut(s) 1742
EcoRII CCWGG 2 cut(s) 329, 788
EcoRV GATATC 1 cut(s) 289
EcoT14I CCWWGG 5 cut(s) 2279, 3453, 3622, 3702, 3717
EcoT22I ATGCAT 3 cut(s) 1671, 3022, 3330
EcoT38I GRGCYC 2 cut(s) 607, 686
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 5 cut(s) 2279, 3453, 3622, 3702, 3717
FaqI GGGAC 1 cut(s) 1065
FauI CCCGC 1 cut(s) 1238
FauNDI CATATG 3 cut(s) 891, 1320, 3697
FbaI TGATCA 7 cut(s) 1096, 1504, 1624, 1966, 2512, 2629, 2638
Fnu4HI GCNGC 6 cut(s) 675, 1527, 2079, 2559, 2970, 3246
FokI GGATG 8 cut(s) 673, 1086, 1341, 1551, 1603, 2155, 2377, 3345
FriOI GRGCYC 2 cut(s) 607, 686
Fsp4HI GCNGC 6 cut(s) 675, 1527, 2079, 2559, 2970, 3246
GlaI GCGC 1 cut(s) 6
GluI GCNGC 6 cut(s) 675, 1527, 2079, 2559, 2970, 3246
GsaI CCCAGC 1 cut(s) 3726
GsuI CTGGAG 1 cut(s) 1326
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 6 cut(s) 221, 272, 329, 1146, 3628, 3862
HapII CCGG 1 cut(s) 1894
HhaI GCGC 1 cut(s) 7
Hin1I GRCGYC 1 cut(s) 5
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HincII GTYRAC 2 cut(s) 2542, 2808
HindII GTYRAC 2 cut(s) 2542, 2808
HindIII AAGCTT 3 cut(s) 1947, 2357, 3484
HinfI GANTC 9 cut(s) 635, 840, 970, 1364, 1688, 2038, 2380, 2390, 3350
HpaI GTTAAC 2 cut(s) 2542, 2808
HpaII CCGG 1 cut(s) 1894
HphI GGTGA 9 cut(s) 296, 1094, 1207, 1307, 1310, 1943, 2696, 3220, 3314
Hpy166II GTNNAC 6 cut(s) 745, 2542, 2808, 2981, 3794, 3883
Hpy8I GTNNAC 6 cut(s) 745, 2542, 2808, 2981, 3794, 3883
HpyCH4III ACNGT 9 cut(s) 64, 231, 361, 559, 714, 2261, 3223, 3889, 3985
Hsp92I GRCGYC 1 cut(s) 5
HspAI GCGC 1 cut(s) 5
KasI GGCGCC 1 cut(s) 4
KpnI GGTACC 1 cut(s) 3970
Ksp22I TGATCA 7 cut(s) 1096, 1504, 1624, 1966, 2512, 2629, 2638
KspAI GTTAAC 2 cut(s) 2542, 2808
LguI GCTCTTC 1 cut(s) 1431
LmnI GCTCC 5 cut(s) 96, 416, 1307, 1523, 1753
Lsp1109I GCAGC 6 cut(s) 661, 1513, 2090, 2545, 2956, 3232
MfeI CAATTG 3 cut(s) 901, 1383, 3256
MflI RGATCY 1 cut(s) 1193
MhlI GDGCHC 2 cut(s) 607, 686
MlsI TGGCCA 1 cut(s) 272
MluNI TGGCCA 1 cut(s) 272
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 4 cut(s) 644, 1358, 1697, 2399
MmeI TCCRAC 2 cut(s) 1501, 3058
Mox20I TGGCCA 1 cut(s) 272
Mph1103I ATGCAT 3 cut(s) 1671, 3022, 3330
MroXI GAANNNNTTC 4 cut(s) 808, 1342, 3048, 3124
MscI TGGCCA 1 cut(s) 272
MslI CAYNNNNRTG 4 cut(s) 1220, 1286, 2069, 2631
Msp20I TGGCCA 1 cut(s) 272
MspA1I CMGCKG 1 cut(s) 2924
MspI CCGG 1 cut(s) 1894
MspR9I CCNGG 2 cut(s) 331, 790
MunI CAATTG 3 cut(s) 901, 1383, 3256
Mva1269I GAATGC 1 cut(s) 3292
MvaI CCWGG 2 cut(s) 331, 790
NarI GGCGCC 1 cut(s) 5
NdeI CATATG 3 cut(s) 891, 1320, 3697
NheI GCTAGC 1 cut(s) 953
NlaIV GGNNCC 5 cut(s) 6, 1550, 1743, 3051, 3968
NmuCI GTSAC 9 cut(s) 24, 199, 636, 923, 944, 1082, 2420, 2748, 3684
NsiI ATGCAT 3 cut(s) 1671, 3022, 3330
NspI RCATGY 6 cut(s) 458, 2238, 2378, 2447, 2612, 3770
OliI CACNNNNGTG 1 cut(s) 1286
PaeI GCATGC 2 cut(s) 458, 2238
PaeR7I CTCGAG 1 cut(s) 366
PagI TCATGA 8 cut(s) 322, 1501, 2263, 2383, 2626, 2632, 2641, 2938
PceI AGGCCT 1 cut(s) 329
PciI ACATGT 2 cut(s) 2374, 2443
PciSI GCTCTTC 1 cut(s) 1431
PctI GAATGC 1 cut(s) 3292
PdmI GAANNNNTTC 4 cut(s) 808, 1342, 3048, 3124
PfeI GAWTC 5 cut(s) 840, 970, 2038, 2380, 3350
PkrI GCNGC 6 cut(s) 676, 1528, 2080, 2560, 2971, 3247
PleI GAGTC 4 cut(s) 643, 1358, 1696, 2398
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 4 cut(s) 643, 1358, 1696, 2398
PpuMI RGGWCCY 1 cut(s) 1742
PscI ACATGT 2 cut(s) 2374, 2443
PshBI ATTAAT 1 cut(s) 1065
PsiI TTATAA 1 cut(s) 3991
Psp124BI GAGCTC 2 cut(s) 607, 686
Psp5II RGGWCCY 1 cut(s) 1742
Psp6I CCWGG 2 cut(s) 329, 788
PspFI CCCAGC 1 cut(s) 3722
PspGI CCWGG 2 cut(s) 329, 788
PspN4I GGNNCC 5 cut(s) 6, 1550, 1743, 3051, 3968
PspPI GGNCC 2 cut(s) 1742, 3626
PspPPI RGGWCCY 1 cut(s) 1742
PstI CTGCAG 3 cut(s) 1043, 2080, 2974
PsuI RGATCY 1 cut(s) 1193
PvuII CAGCTG 1 cut(s) 2924
RsaI GTAC 8 cut(s) 648, 733, 1707, 1725, 2622, 2982, 3793, 3968
RsaNI GTAC 8 cut(s) 647, 732, 1706, 1724, 2621, 2981, 3792, 3967
RseI CAYNNNNRTG 4 cut(s) 1220, 1286, 2069, 2631
SacI GAGCTC 2 cut(s) 607, 686
SapI GCTCTTC 1 cut(s) 1431
SatI GCNGC 6 cut(s) 675, 1527, 2079, 2559, 2970, 3246
Sau96I GGNCC 2 cut(s) 1742, 3626
SchI GAGTC 4 cut(s) 644, 1358, 1697, 2399
ScrFI CCNGG 2 cut(s) 331, 790
SduI GDGCHC 2 cut(s) 607, 686
SfcI CTRYAG 6 cut(s) 691, 1039, 1075, 2076, 2338, 2970
SfoI GGCGCC 1 cut(s) 6
Sfr274I CTCGAG 1 cut(s) 366
SinI GGWCC 1 cut(s) 1742
SlaI CTCGAG 1 cut(s) 366
SmiI ATTTAAAT 1 cut(s) 3463
SmiMI CAYNNNNRTG 4 cut(s) 1220, 1286, 2069, 2631
SmlI CTYRAG 2 cut(s) 366, 3153
SmoI CTYRAG 2 cut(s) 366, 3153
SpeI ACTAGT 1 cut(s) 203
SphI GCATGC 2 cut(s) 458, 2238
SseBI AGGCCT 1 cut(s) 329
SsiI CCGC 5 cut(s) 1134, 1218, 1231, 2525, 2552
SspDI GGCGCC 1 cut(s) 4
SspI AATATT 4 cut(s) 1012, 1903, 2829, 3073
SstI GAGCTC 2 cut(s) 607, 686
StuI AGGCCT 1 cut(s) 329
StyD4I CCNGG 2 cut(s) 329, 788
StyI CCWWGG 5 cut(s) 2279, 3453, 3622, 3702, 3717
SwaI ATTTAAAT 1 cut(s) 3463
TaaI ACNGT 9 cut(s) 64, 231, 361, 559, 714, 2261, 3223, 3889, 3985
TaqI TCGA 3 cut(s) 345, 367, 1794
TatI WGTACW 1 cut(s) 1705
TfiI GAWTC 5 cut(s) 840, 970, 2038, 2380, 3350
TscAI CASTG 8 cut(s) 190, 594, 765, 1119, 1288, 2425, 3604, 3894
TseFI GTSAC 9 cut(s) 24, 199, 636, 923, 944, 1082, 2420, 2748, 3684
TseI GCWGC 6 cut(s) 674, 1526, 2078, 2558, 2969, 3245
Tsp45I GTSAC 9 cut(s) 24, 199, 636, 923, 944, 1082, 2420, 2748, 3684
TspRI CASTG 8 cut(s) 190, 594, 765, 1119, 1288, 2425, 3604, 3894
VpaK11BI GGWCC 1 cut(s) 1742
VspI ATTAAT 1 cut(s) 1065
XapI RAATTY 6 cut(s) 1808, 2647, 2656, 3431, 3464, 3942
XceI RCATGY 6 cut(s) 458, 2238, 2378, 2447, 2612, 3770
XcmI CCANNNNNNNNNTGG 1 cut(s) 1922
XhoI CTCGAG 1 cut(s) 366
XmnI GAANNNNTTC 4 cut(s) 808, 1342, 3048, 3124
Zsp2I ATGCAT 3 cut(s) 1671, 3022, 3330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.