Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0001471

Physical Location & Seq

Genomic Coordinates Reverse (-)

19276472 .. 19281145

Gene Structure 4674 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G010970.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2151 bp

ATGGCTTTAATGAGTCTGCTTATGGCAACACTGGAGGAAGATATTGACAAGGCATCCAAAACAGTTCTCTATCAAGGAAAGAGTAGTGAAGAAGGTCTTTTTCTCTTCAAGACACCACCAGTTTCTTCAATACCGGAACTATCTCACACTGCCTTTGTTAGCTCTTCCACATCATATCCAAAGGCAAGATGCATAGATTACCTTTTACTGAATCTGTATCCAAAAGCCCTCTGCCTTTTCATAAAAATGCCTTGCAAATCATTGAATATGATTTCACCCTATGAGCTTGTGTTCCATAAAGTTCCAGATATCTCTCAGCTTAAAATCTTTGGTTCCTCGTGTTATCCATATCTTAGACCCTATAATCATGACAAGTTAGATCCCAGAACTACTAAGTGTGTGTTCTTGGGATATGCTCTAGGGTACAAAGGTGTATTCTGCTACAATATTCCTCACAATAAACTCTGGCTGTCTAGACATGTAATCCATGATGAAACCAGTTTTCCCTACTTGTCTTCTACTTACCCTGTGTCAAACCCACCTGATCCAGCCACCTTCTTTCCCTCTTCTGCAACCTTCACTACTCTTCCCCATCAGATGCCTGTCCTTGCTTCTCATGGTCCACTCAGTGAGGTCCCTACTGAGTTGGAGGGGGACAATGGTGGTAGTTTAGGTAATTCGAGTAGTGGTGTGATTGGTAATGCAGGTGATGCAGGTGGTGATATGAGTGGAGATATGAACATAGAAGTTGCTCTTCAGGATGAGGTTAGTGATGCTCTAAATGATCAGAATGATCAAAATGAAGTTAATTCGCATAATGAAGCTAGTAGTGAGGCAAGCATTAGTGGTGATACAAATGATGATCAACATGAAGTTCATATGCAGAATGAGGTTAGTAGTGATGAAAATGTTGGTGTTAATGAGGTAGGAATTGCTGAGCAGAATGCTTCTTCAGATATTGATATTTCAATTGGTGGTCTTAATGCAAATGAAAATAATGCTTTACAGTTTGCGAGGTTGGTAGCTCAAGGTTTTAGTCAAGCCAAAGGTCTAGATTATGATGAGACCTTTAGTCCTGTTGTGAGACATAGCACTGTGAGAATTATATTGGCTCTTGCTGCCATGAACAATTGGGAGTTGAGACAATTGGATGTTAAAAATGCCTTTTTACATGGAGACTTAAAGGAAGAGGTGTATATGGTTCAACCACAAGGTTTTGTCGACTCTCTGCACCCTGATTATGTATGCTTGTTAAGAAAATCTCTCTATGGTCTAAAGCAAGCTCCAAGAGCATGGAATGAGAAGTTTACTAGTTTCTTACCAAGCTCTGACACTACAGGTATTCGTGAGGTTGTCTCTGAGCTGAGCATGGTGTTTGACATGAAGGATCTAGGTGCCTTAACTTACTTTCTTGGACTGGAGGTGACCAAAGTTCAACGAGGCATATTCCTGAGTCAAACTAAATATGCTAAAGATTTGCTTGCCAAGACAGGAATGGATACTATTAAAGCCTGCAGCTCACCTTGCCTTCCTCACTATCAAATGACAAAAGATCAAGATGTTCATTATGCTGCAGTCAAAAGGATTTTGAGGTATTTGCAAGGATCTTTGCATAAAGGGTTGTTCTATAGCTCTAATGGTGCCTTGAATAATGCTGTGTGTGTTAAGGCCTTTTGTGATGCTGATTGGGCTGGTGAAGTCATACAAAGGCGTTCTACTACAGGATTTGTTGTCTATCTTGGCTTGTGTCCTGTATCTTGGCAGTCCAAGAAGCAAGGTACTGTGTCACGTAGCTCTACTGAATCTGAATACAGGAGTTTAGCTAACACTGCTGCTGAGATTAGTTGGATCAGACACTTGTTATGTGATTTGAAAATTCAGATTCCTCGTGCTCCTTTGCTTAAATGTGACAATTTGTCAGCATTGGCCTTGGCATCTAATCCTGTGTTTCATTCCAAGATCAAGCATTTGGACAATGACTTTCACTTTGTTCGTGAACGTGTGCAGCATAATGATCTTTGTCTTGAATATGTGCCTACTACAGAACAAACTGCAGATATTTTGACAAAAGGGTTACCTTCACCAATCTTTCGAACACACACTATCAATCTCAGACTAGTGACCCTAGCTGAGCTTGAGGAGGGGTGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RVT_2 Retrotran_gag_2 Retrotran_gag_3 Retrotrans_gag gag_pre-integrs rve

Protein Analysis

716

Amino Acids

79.26

Weight (kDa)

5.55

Isoelectric Point (pI)

46.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SH3_retrovirus	PF25597	112 - 173	2.7e-17	Retroviral polymerase SH3-like domain
RVT_2	PF07727	337 - 440	9.8e-38	Reverse transcriptase (RNA-dependent DNA polymerase)
RVT_2	PF07727	439 - 510	2.9e-08	Reverse transcriptase (RNA-dependent DNA polymerase)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001471)

Species	Orthologous Gene IDs
arabidopsis_thaliana	ATMG00240
fragaria_vesca	FvH4_1g25543 FvH4_3g07841 FvH4_3g14871 FvH4_4g13042 FvH4_4g19532 FvH4_4g27904 FvH4_5g11311 FvH4_5g24711 FvH4_6g16681
malus_domestica	MD01G1044800.v1.1 MD05G1032500.v1.1 MD14G1067200.v1.1
pyrus_communis	pycom02g08540 pycom02g19080 pycom03g19430 pycom05g02910 pycom15g04590 pycom16g02160 pycom16g25210
rosa_chinensis	RchiOBHm_Chr1g0313691 RchiOBHm_Chr1g0339121 RchiOBHm_Chr2g0119341 RchiOBHm_Chr2g0141981 RchiOBHm_Chr2g0155191 RchiOBHm_Chr2g0175291 RchiOBHm_Chr3g0475621 RchiOBHm_Chr3g0482011 RchiOBHm_Chr4g0401001 RchiOBHm_Chr5g0038401 RchiOBHm_Chr6g0253961 RchiOBHm_Chr6g0266711 RchiOBHm_Chr7g0198221 RchiOBHm_Chr7g0200081
rosa_laevigata	RLG00000035859
rosa_roxburghii	Rroxscaffold_4G00281750
rosa_wichuraiana	Rw1G008180 Rw1G040520 Rw2G014210 Rw2G020850 Rw2G043190 Rw2G049320 Rw4G005820 Rw4G021520 Rw5G011150 Rw5G016420 Rw6G010970 Rw7G015120 Rw7G015940 Rw7G019550 Rw7G023080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	695, 704
Acc36I	ACCTGC	2 cut(s)	695, 704
AccB1I	GGYRCC	2 cut(s)	1394, 1640
AccI	GTMKAC	1 cut(s)	1221
AclWI	GGATC	5 cut(s)	374, 539, 1395, 1612, 1856
AcsI	RAATTY	1 cut(s)	1875
AcuI	CTGAAG	2 cut(s)	740, 936
AdeI	CACNNNGTG	1 cut(s)	629
AfaI	GTAC	2 cut(s)	425, 1780
AflIII	ACRYGT	2 cut(s)	478, 1999
AgsI	TTSAA	9 cut(s)	109, 129, 265, 969, 1205, 1436, 1648, 1873, 2027
AhdI	GACNNNNNGTC	2 cut(s)	1071, 1915
AhlI	ACTAGT	2 cut(s)	1310, 2116
Alw21I	GWGCWC	1 cut(s)	1894
Alw26I	GTCTC	5 cut(s)	1058, 1078, 1135, 1170, 1360
AlwI	GGATC	5 cut(s)	374, 539, 1395, 1612, 1856
AoxI	GGCC	2 cut(s)	1668, 1926
ApeKI	GCWGC	5 cut(s)	1118, 1515, 1571, 1832, 2005
ApoI	RAATTY	1 cut(s)	1875
ArsI	GACNNNNNNTTYG	2 cut(s)	1970, 2002
AspS9I	GGNCC	2 cut(s)	620, 634
AsuHPI	GGTGA	8 cut(s)	267, 719, 731, 860, 1435, 1512, 1706, 2073
AsuII	TTCGAA	1 cut(s)	2092
AvaII	GGWCC	2 cut(s)	620, 634
BaeI	ACNNNNGTAYC	2 cut(s)	415, 448
BanI	GGYRCC	2 cut(s)	1394, 1640
BauI	CACGAG	2 cut(s)	337, 1887
BbsI	GAAGAC	1 cut(s)	507
Bbv12I	GWGCWC	1 cut(s)	1894
BbvI	GCAGC	5 cut(s)	1105, 1527, 1558, 1819, 2017
BccI	CCATC	1 cut(s)	600
BciVI	GTATCC	2 cut(s)	228, 1492
BclI	TGATCA	3 cut(s)	784, 793, 862
BcoDI	GTCTC	5 cut(s)	1058, 1078, 1135, 1170, 1360
BcuI	ACTAGT	2 cut(s)	1310, 2116
BfaI	CTAG	8 cut(s)	419, 474, 825, 1052, 1311, 1391, 2117, 2126
BfmI	CTRYAG	7 cut(s)	1335, 1513, 1572, 1627, 1719, 2040, 2052
BfuAI	ACCTGC	2 cut(s)	695, 704
BfuI	GTATCC	2 cut(s)	228, 1492
BisI	GCNGC	5 cut(s)	1119, 1516, 1572, 1833, 2006
BlpI	GCTNAGC	3 cut(s)	936, 1364, 2130
BlsI	GCNGC	5 cut(s)	1120, 1517, 1573, 1834, 2007
Bme18I	GGWCC	2 cut(s)	620, 634
BmeRI	GACNNNNNGTC	2 cut(s)	1071, 1915
BmgT120I	GGNCC	2 cut(s)	620, 634
BmiI	GGNNCC	4 cut(s)	334, 636, 1396, 1642
BmsI	GCATC	7 cut(s)	62, 179, 588, 700, 763, 1669, 1943
BpiI	GAAGAC	1 cut(s)	507
BplI	GAGNNNNNCTC	2 cut(s)	1340, 1372
BpmI	CTGGAG	2 cut(s)	53, 1439
Bpu1102I	GCTNAGC	3 cut(s)	936, 1364, 2130
Bpu14I	TTCGAA	1 cut(s)	2092
BpuEI	CTTGAG	1 cut(s)	1011
BsaAI	YACGTR	1 cut(s)	1790
BsaI	GGTCTC	1 cut(s)	1058
BsaJI	CCNNGG	1 cut(s)	1929
BsaWI	WCCGGW	1 cut(s)	133
Bse1I	ACTGG	4 cut(s)	36, 119, 498, 1422
BseDI	CCNNGG	1 cut(s)	1929
BseGI	GGATG	3 cut(s)	53, 766, 1156
BseNI	ACTGG	4 cut(s)	36, 119, 498, 1422
BseXI	GCAGC	5 cut(s)	1105, 1527, 1558, 1819, 2017
BsgI	GTGCAG	2 cut(s)	1214, 2024
BshFI	GGCC	2 cut(s)	1670, 1928
BshNI	GGYRCC	2 cut(s)	1394, 1640
BsiHKAI	GWGCWC	1 cut(s)	1894
BsiSI	CCGG	1 cut(s)	134
BslFI	GGGAC	2 cut(s)	620, 668
BsmAI	GTCTC	5 cut(s)	1058, 1078, 1135, 1170, 1360
BsmFI	GGGAC	2 cut(s)	620, 668
BsmI	GAATGC	1 cut(s)	949
BsnI	GGCC	2 cut(s)	1670, 1928
Bso31I	GGTCTC	1 cut(s)	1058
Bsp119I	TTCGAA	1 cut(s)	2092
Bsp1286I	GDGCHC	1 cut(s)	1894
Bsp1720I	GCTNAGC	3 cut(s)	936, 1364, 2130
BspANI	GGCC	2 cut(s)	1670, 1928
BspHI	TCATGA	1 cut(s)	367
BspLI	GGNNCC	4 cut(s)	334, 636, 1396, 1642
BspMAI	CTGCAG	3 cut(s)	1517, 1576, 2056
BspMI	ACCTGC	2 cut(s)	695, 704
BspPI	GGATC	5 cut(s)	374, 539, 1395, 1612, 1856
BspQI	GCTCTTC	2 cut(s)	169, 759
BspT104I	TTCGAA	1 cut(s)	2092
BspT107I	GGYRCC	2 cut(s)	1394, 1640
BspTNI	GGTCTC	1 cut(s)	1058
BsrI	ACTGG	4 cut(s)	36, 119, 498, 1422
BssECI	CCNNGG	1 cut(s)	1929
BssSI	CACGAG	2 cut(s)	337, 1887
BssT1I	CCWWGG	1 cut(s)	1929
Bst2BI	CACGAG	2 cut(s)	337, 1887
Bst4CI	ACNGT	4 cut(s)	64, 1008, 1096, 1783
Bst6I	CTCTTC	6 cut(s)	110, 169, 571, 591, 759, 1182
BstAPI	GCANNNNNTGC	1 cut(s)	710
BstBAI	YACGTR	1 cut(s)	1790
BstBI	TTCGAA	1 cut(s)	2092
BstC8I	GCNNGC	4 cut(s)	838, 1281, 1482, 1513
BstEII	GGTNACC	2 cut(s)	1423, 2073
BstF5I	GGATG	3 cut(s)	53, 766, 1156
BstMAI	GTCTC	5 cut(s)	1058, 1078, 1135, 1170, 1360
BstMWI	GCNNNNNNNGC	6 cut(s)	710, 1118, 1289, 1524, 1688, 1829
BstNSI	RCATGY	1 cut(s)	482
BstPI	GGTNACC	2 cut(s)	1423, 2073
BstSFI	CTRYAG	7 cut(s)	1335, 1513, 1572, 1627, 1719, 2040, 2052
BstV1I	GCAGC	5 cut(s)	1105, 1527, 1558, 1819, 2017
BstV2I	GAAGAC	1 cut(s)	507
BstX2I	RGATCY	3 cut(s)	379, 1387, 1604
BstXI	CCANNNNNNTGG	1 cut(s)	1293
BstYI	RGATCY	3 cut(s)	379, 1387, 1604
BsuI	GTATCC	2 cut(s)	228, 1492
BsuRI	GGCC	2 cut(s)	1670, 1928
BtsCI	GGATG	3 cut(s)	53, 766, 1156
BtsI	GCAGTG	2 cut(s)	147, 1827
BtsIMutI	CAGTG	5 cut(s)	29, 147, 634, 1092, 1827
BveI	ACCTGC	2 cut(s)	695, 704
Cac8I	GCNNGC	4 cut(s)	838, 1281, 1482, 1513
CciI	TCATGA	1 cut(s)	367
Cfr13I	GGNCC	2 cut(s)	620, 634
Csp6I	GTAC	2 cut(s)	424, 1779
CviQI	GTAC	2 cut(s)	424, 1779
DraIII	CACNNNGTG	1 cut(s)	629
DriI	GACNNNNNGTC	2 cut(s)	1071, 1915
Eam1104I	CTCTTC	6 cut(s)	110, 169, 571, 591, 759, 1182
Eam1105I	GACNNNNNGTC	2 cut(s)	1071, 1915
EarI	CTCTTC	6 cut(s)	110, 169, 571, 591, 759, 1182
Eco130I	CCWWGG	1 cut(s)	1929
Eco147I	AGGCCT	1 cut(s)	1670
Eco31I	GGTCTC	1 cut(s)	1058
Eco32I	GATATC	1 cut(s)	310
Eco47I	GGWCC	2 cut(s)	620, 634
Eco57I	CTGAAG	2 cut(s)	740, 936
Eco91I	GGTNACC	2 cut(s)	1423, 2073
EcoO109I	RGGNCCY	1 cut(s)	634
EcoO65I	GGTNACC	2 cut(s)	1423, 2073
EcoRV	GATATC	1 cut(s)	310
EcoT14I	CCWWGG	1 cut(s)	1929
EcoT22I	ATGCAT	1 cut(s)	194
ErhI	CCWWGG	1 cut(s)	1929
FalI	AAGNNNNNCTT	6 cut(s)	81, 113, 738, 770, 1464, 1496
FaqI	GGGAC	2 cut(s)	620, 668
FauNDI	CATATG	1 cut(s)	879
FbaI	TGATCA	3 cut(s)	784, 793, 862
FblI	GTMKAC	1 cut(s)	1221
Fnu4HI	GCNGC	5 cut(s)	1119, 1516, 1572, 1833, 2006
FokI	GGATG	3 cut(s)	40, 773, 1163
Fsp4HI	GCNGC	5 cut(s)	1119, 1516, 1572, 1833, 2006
FspBI	CTAG	8 cut(s)	419, 474, 825, 1052, 1311, 1391, 2117, 2126
GluI	GCNGC	5 cut(s)	1119, 1516, 1572, 1833, 2006
GsuI	CTGGAG	2 cut(s)	53, 1439
HaeIII	GGCC	2 cut(s)	1670, 1928
HapII	CCGG	1 cut(s)	134
HincII	GTYRAC	1 cut(s)	1222
HindII	GTYRAC	1 cut(s)	1222
HinfI	GANTC	6 cut(s)	13, 211, 1223, 1453, 1802, 1882
HpaII	CCGG	1 cut(s)	134
HphI	GGTGA	8 cut(s)	267, 719, 731, 860, 1435, 1512, 1706, 2073
Hpy166II	GTNNAC	4 cut(s)	623, 1222, 1308, 1997
Hpy188I	TCNGA	9 cut(s)	597, 789, 955, 1330, 1360, 1807, 1853, 1881, 2114
Hpy8I	GTNNAC	4 cut(s)	623, 1222, 1308, 1997
HpyAV	CCTTC	6 cut(s)	86, 565, 586, 1378, 1538, 2088
HpyCH4III	ACNGT	4 cut(s)	64, 1008, 1096, 1783
HpyCH4IV	ACGT	2 cut(s)	1789, 1999
HpyF10VI	GCNNNNNNNGC	6 cut(s)	710, 1118, 1289, 1524, 1688, 1829
HpySE526I	ACGT	2 cut(s)	1789, 1999
Ksp22I	TGATCA	3 cut(s)	784, 793, 862
LguI	GCTCTTC	2 cut(s)	169, 759
LmnI	GCTCC	2 cut(s)	1288, 1897
Lsp1109I	GCAGC	5 cut(s)	1105, 1527, 1558, 1819, 2017
LweI	GCATC	7 cut(s)	62, 179, 588, 700, 763, 1669, 1943
MaeI	CTAG	8 cut(s)	419, 474, 825, 1052, 1311, 1391, 2117, 2126
MaeII	ACGT	2 cut(s)	1789, 1999
MaeIII	GTNAC	5 cut(s)	1423, 1785, 1907, 2073, 2119
MfeI	CAATTG	3 cut(s)	969, 1129, 1145
MflI	RGATCY	3 cut(s)	379, 1387, 1604
MhlI	GDGCHC	1 cut(s)	1894
MluCI	AATT	9 cut(s)	676, 808, 930, 969, 1101, 1129, 1145, 1875, 1912
MlyI	GAGTC	3 cut(s)	22, 1217, 1462
MmeI	TCCRAC	2 cut(s)	627, 1826
Mph1103I	ATGCAT	1 cut(s)	194
MslI	CAYNNNNRTG	1 cut(s)	245
MspI	CCGG	1 cut(s)	134
MunI	CAATTG	3 cut(s)	969, 1129, 1145
Mva1269I	GAATGC	1 cut(s)	949
MwoI	GCNNNNNNNGC	6 cut(s)	710, 1118, 1289, 1524, 1688, 1829
NdeI	CATATG	1 cut(s)	879
NlaIV	GGNNCC	4 cut(s)	334, 636, 1396, 1642
NmuCI	GTSAC	4 cut(s)	1423, 1785, 1907, 2119
NsiI	ATGCAT	1 cut(s)	194
NspI	RCATGY	1 cut(s)	482
NspV	TTCGAA	1 cut(s)	2092
PagI	TCATGA	1 cut(s)	367
PaqCI	CACCTGC	2 cut(s)	695, 704
PceI	AGGCCT	1 cut(s)	1670
PciI	ACATGT	1 cut(s)	478
PciSI	GCTCTTC	2 cut(s)	169, 759
PctI	GAATGC	1 cut(s)	949
PfeI	GAWTC	3 cut(s)	211, 1802, 1882
PkrI	GCNGC	5 cut(s)	1120, 1517, 1573, 1834, 2007
PleI	GAGTC	3 cut(s)	21, 1217, 1461
PpsI	GAGTC	3 cut(s)	21, 1217, 1461
Ppu21I	YACGTR	1 cut(s)	1790
PpuMI	RGGWCCY	1 cut(s)	634
PscI	ACATGT	1 cut(s)	478
Psp5II	RGGWCCY	1 cut(s)	634
PspEI	GGTNACC	2 cut(s)	1423, 2073
PspN4I	GGNNCC	4 cut(s)	334, 636, 1396, 1642
PspPI	GGNCC	2 cut(s)	620, 634
PspPPI	RGGWCCY	1 cut(s)	634
PstI	CTGCAG	3 cut(s)	1517, 1576, 2056
PsuI	RGATCY	3 cut(s)	379, 1387, 1604
RsaI	GTAC	2 cut(s)	425, 1780
RsaNI	GTAC	2 cut(s)	424, 1779
RseI	CAYNNNNRTG	1 cut(s)	245
SalI	GTCGAC	1 cut(s)	1220
SapI	GCTCTTC	2 cut(s)	169, 759
SatI	GCNGC	5 cut(s)	1119, 1516, 1572, 1833, 2006
Sau96I	GGNCC	2 cut(s)	620, 634
SchI	GAGTC	3 cut(s)	22, 1217, 1462
SduI	GDGCHC	1 cut(s)	1894
SfaNI	GCATC	7 cut(s)	62, 179, 588, 700, 763, 1669, 1943
SfcI	CTRYAG	7 cut(s)	1335, 1513, 1572, 1627, 1719, 2040, 2052
SfuI	TTCGAA	1 cut(s)	2092
SinI	GGWCC	2 cut(s)	620, 634
SmiMI	CAYNNNNRTG	1 cut(s)	245
SmlI	CTYRAG	2 cut(s)	1026, 2135
SmoI	CTYRAG	2 cut(s)	1026, 2135
SpeI	ACTAGT	2 cut(s)	1310, 2116
Sse9I	AATT	9 cut(s)	676, 808, 930, 969, 1101, 1129, 1145, 1875, 1912
SseBI	AGGCCT	1 cut(s)	1670
SspI	AATATT	1 cut(s)	448
SspMI	CTAG	8 cut(s)	419, 474, 825, 1052, 1311, 1391, 2117, 2126
StuI	AGGCCT	1 cut(s)	1670
StyI	CCWWGG	1 cut(s)	1929
TaaI	ACNGT	4 cut(s)	64, 1008, 1096, 1783
TaiI	ACGT	2 cut(s)	1792, 2002
TaqI	TCGA	3 cut(s)	680, 1221, 2092
TasI	AATT	9 cut(s)	676, 808, 930, 969, 1101, 1129, 1145, 1875, 1912
TfiI	GAWTC	3 cut(s)	211, 1802, 1882
TscAI	CASTG	5 cut(s)	36, 154, 634, 1099, 1834
TseFI	GTSAC	4 cut(s)	1423, 1785, 1907, 2119
TseI	GCWGC	5 cut(s)	1118, 1515, 1571, 1832, 2005
Tsp45I	GTSAC	4 cut(s)	1423, 1785, 1907, 2119
TspRI	CASTG	5 cut(s)	36, 154, 634, 1099, 1834
VpaK11BI	GGWCC	2 cut(s)	620, 634
XapI	RAATTY	1 cut(s)	1875
XbaI	TCTAGA	2 cut(s)	473, 1051
XceI	RCATGY	1 cut(s)	482
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1492
XmiI	GTMKAC	1 cut(s)	1221
XspI	CTAG	8 cut(s)	419, 474, 825, 1052, 1311, 1391, 2117, 2126
Zsp2I	ATGCAT	1 cut(s)	194

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.