RchiOBHm_Chr3g0475621
RLK Family

cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0001471
Physical Location & Seq
Forward (+)
21457576 .. 21462974
5399 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ44112

Sequence Viewer

Length: 3444 bp
ATGAGTGCTCATTCATCTGGATCATGTTCATCTGGATCTTCTGGAGCATGTTCATCTGGATCTCAGGTTAATGAAGTATGGATTGGTGATACTGGTGCCACTCACCATATGACTTCTGATCTCAGGAACCTCACCATTGCTCATCCTTTTGAGTCTTCCAAGTCGATTACCATTGGCAATGGTGAAGGTTTGCATATCAAACATATTGGCTCTGCAATTATTACACCTGCTGATCATACATTCAAGCTTAACAATGTGTTACATGTTCCTCATTTGGCAGTCAATTTACTATCATTTAACAAACTCTGCAAGGACAATCATTGCTTTATTGTCATGGATGATGTTGATATATGTGTGCAGGACAAGGCATCAAAGGCACTACTGTACAAAGGAAAGAGTAATGGGGAAGGACTCTTCTTGTTCAAAACACCAAATCTCACACAACCTTATAATCATTCTCACACTGCCTTTATTGGATCAGCTGTCAAGTCTACAATCTGGCATCAAAGACTTGGTCATCGATCTCCTGATATTGTTTCAAGGATGCTATCTCAGTCTAGGATAACATACAATAATGATACTTCTACTCATATGTGTGTTGATTGCCTTAGTGGTAAGATGCATAGGTTACCATTCTCTGAGTCTGTATCTCAATCTGTATCACCTTTTCAAAAAATTCATAGTGATCTCTGGGGTCCAGCTCCATGCTTGTCTGTTGAAGGCTTCAAGTTTTATGTAACCTTCATTGATGACTATACCAAATTTGTATGGATATATCCACTCATCAATAAATCTGATACTTTCTCCACTTTTGTTAAGTTCTATAACTTTGTGAGTACTCAGTTTCATATCCTTATTCAATCTCTTCAAACTGATGGAGGAGGTGAGTTCAATAGCAAGGCATTTCAATCTTTTCTTGATAGCAAAGGGATTCTGCACCTCATTACATGTCCATATACTCCTCAGCAGAATGGGGTTGCAGAACGCAAAAATAGACACCTTGTTGAAACATGCATTTCTTTGATGTCTGCTGCTAAGTTCCCTAAACCATTCTGGTTCCATGCTGTGGCACATTCTTCCAATCTCATTAACAGGATGCCATGCAAATCTCTTCTGTACCACTCTCCATATTTTAAGCTCTTTGGTGTGCTACCTGATTTGAAGTCTCTCAAGGTATTTGGTACTGCTTGTTATCCTTATCTTAGACCTTATTCTCATGATAAACTTGATCCAAGGACTACTATGTGTGTTTTCTTGGGATATGCTCTAGGCTATAAGGGTGTTTTCTGCTATAACATTGAACATCACAAGTTATGGATGTGTCGACATGTGATTCATGATGAATCTACTTTTCCTTTTAAGTGTCCTCCTTCTCCACCTTGTGCTTCTGCTCCTTCTTCACCATCATCTGTTGCTTTTTCTACCTCACCTTCACCTTCACCTGCTTCTCTTCCTACTGCTTCTATCTCCTCTGGTCATTCCTCCATGTTGCCTGTTCTCACTTCTGATCAGTTACAAGTTGTTCTTCCTTGTGAGCCTGTTTCTCAGCCTTCTGCTGCTATCCCAGCCTTGGCTGATGGGGGCGGTGTTAGTAGAGACGCAAGTTCTAGCTCATCAGAATCATCTGATTCTATGACTAATGGTGATATATCTAATAATGATCTGCCTAGTGATAGTGATAATGGTGGTCTTCCACAAGAGCTAGTGTTTGTTGAAGATCAAGCTCAGGCTTACAATAATCACTCTATGTTAACTAGAGCCAAGAATGGAATTGTGAAACATAAGACATTTCAAGACTATTGTGCTTATTCCTGTGTGGTTCAACAAGGTCTTATCGATGAACTTGCCTATTTTAGTGGCTTTTCTGCTATCATGGATATACAAGATCCTATTGAACCAAAATCGTTTAGAGCTGCTGCAAGTATACCTGAATGGGATGAGGCTATGTATGAGGAGATTGATGCTTTGTTGAAACAAGGTACTTGGGAACTTGTCCCTTTACCTAAAGCAAAGAACATTGTTGGGAGCAAATGGATTTACAAGATCAAGAAAAATCCAGATGGATCAATTTCAAGGTATAAAGCCAGACTTGTTGCTCAAGGATTTAGTCAAGAAAAAGGATTGGATTATGATGAAACTTTCAGTCCTGTGGTGAGACATAGTACTGTTAGGCTTGTAGTTGCTCTTGCTGCAATGAACAAATGGGAGTTGCGACAATTGGATGTTAAAAATGCTTTTCTGCATGGTGATCTTAGGGAAGAAGTATACATGGTACAGCCACAGGGCTTTGTTGACTCTAAACATCCTGATCATGTGTGCTTGTTGAAGAAATCACTCTATGGTCTAAAACAAGCTCCTAGAGCTTGGAATGAGAAGTTTACTAATTTCCTGCCAGCTCTAGGATTCCAGTTTTCACATTATGATCCTAGCCTGTTTGTCAAGCATTCTGAAAAAGGTGTGATAATCTTACTTCTGTATGTAGACGATATTGTCATAACTGGGTCAGACAAGGAGAGTATTGAAGCTGTGATTACTGAGTTAAGTGAAGCTTTTGATATGAAGGACTTAGGTTCATTGTCTTATTTCTTGGGAATCAATGTTCAATACAAGACTACTGGAATTCTTCTATCTCAAGAAAAATATGCTAAGGAGTTGATTGCAAAAGCTGGCTTAGAAACATGTAGAGAGTGTGTATCTCCTTGTTTACCACATGTTCAGTTATTGAAAAATGATGGTAATCCACTGGCTAATCCTACACTTTACAGGAGCATTGTTGGAGCACTCCAATACTTGACATTTACAAGACCTGACATTGCATTCGCTGTCAATACTGTGTGTCAGTTTATGTCAACACCAACTGATGTACATTTTGCTTCTGTAAAAAGGATTCTCAGGTATATTCAAGGCACTCTCTCAAAAAGAATGTTTTACAAGTATGGCAATGGTGTTCCATATATTAATGCCTATTGTGATGCTGACTGGGCTGGTGAAGTTAACCATAGAAGATCAACCACAGGGTTTATTGTTTATCTCGCTCACTGTCCTATATCTTGGCAATCAAAGAAACAAGGATCTGTGTCAAGAAGTTCCACAGAAGCTGAGTATAGAGCATTGGCTAATACGACTGCAGAAATCTCCTGGCTGAGGCATGTCTTATGTGATCTGCATATTCGAATTCCTGCACCACCTCTACTAAAATGTGATAATCTTTCTGCACTTGCTCTTTGTTCAAATCCTGTGTTTCACTCAAGAATTAAGCATTTGGACAATGACTTTCATTTTGTGCGTGAAAGAGTTCAGAGGCAGGATTTATTACTTCAGTATGTTCCAACTGAGCAACAGACTGCTGATATCTTCACTAAGGGATTGCATAGTCCACTGTTCAAATCTCATTGCTCCAATCTCAGTCTGGTATCCCCTACTGAGATTGAGGGGAGGTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2 Retrotran_gag_3 Retrotrans_gag gag_pre-integrs rve

Protein Analysis

1147

Amino Acids

127.64

Weight (kDa)

7.7

Isoelectric Point (pI)

47.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pol_BBD PF22936 27 - 105 2.6e-14 Pol polyprotein, beta-barrel domain
gag_pre-integrs PF13976 138 - 207 2.4e-08 GAG-pre-integrase domain
rve PF00665 221 - 321 5.3e-11 Integrase core domain
SH3_retrovirus PF25597 396 - 456 3.5e-15 Retroviral polymerase SH3-like domain
RVT_2 PF07727 661 - 902 1.2e-78 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 450
AarI CACCTGC 2 cut(s) 235, 1450
AasI GACNNNNNNGTC 1 cut(s) 2489
Acc36I ACCTGC 2 cut(s) 235, 1450
AccB1I GGYRCC 1 cut(s) 95
AccB7I CCANNNNNTGG 1 cut(s) 1066
AccI GTMKAC 5 cut(s) 491, 1324, 1924, 2265, 2481
AciI CCGC 1 cut(s) 1584
AclWI GGATC 9 cut(s) 28, 43, 67, 484, 1223, 1880, 2071, 2417, 3079
AcsI RAATTY 4 cut(s) 675, 761, 2619, 3174
AcuI CTGAAG 1 cut(s) 3302
AdeI CACNNNGTG 1 cut(s) 1382
AfaI GTAC 8 cut(s) 386, 838, 1118, 1183, 1981, 2164, 2274, 2865
AfiI CCNNNNNNNGG 4 cut(s) 1066, 1570, 2399, 3144
AflIII ACRYGT 5 cut(s) 262, 947, 1327, 2678, 2710
AjnI CCWGG 1 cut(s) 3137
AjuI GAANNNNNNNTTGG 2 cut(s) 66, 98
AloI GAACNNNNNNTCC 2 cut(s) 2583, 2615
Alw21I GWGCWC 2 cut(s) 10, 2782
Alw26I GTCTC 3 cut(s) 1170, 1590, 2149
AlwI GGATC 9 cut(s) 28, 43, 67, 484, 1223, 1880, 2071, 2417, 3079
AlwNI CAGNNNCTG 1 cut(s) 3098
ApeKI GCWGC 5 cut(s) 1031, 1556, 1913, 1916, 2189
ApoI RAATTY 4 cut(s) 675, 761, 2619, 3174
ArsI GACNNNNNNTTYG 6 cut(s) 499, 531, 2801, 2833, 3263, 3295
AseI ATTAAT 1 cut(s) 2958
Asp700I GAANNNNTTC 1 cut(s) 3294
AspS9I GGNCC 1 cut(s) 695
AsuII TTCGAA 1 cut(s) 3172
AvaII GGWCC 1 cut(s) 695
BanI GGYRCC 1 cut(s) 95
BbsI GAAGAC 2 cut(s) 147, 1682
Bbv12I GWGCWC 2 cut(s) 10, 2782
BbvCI CCTCAGC 2 cut(s) 963, 3143
BbvI GCAGC 5 cut(s) 1018, 1543, 1900, 1903, 2176
BccI CCATC 5 cut(s) 869, 1411, 1571, 2054, 2726
BcgI CGANNNNNNTGC 2 cut(s) 1826, 1860
BciT130I CCWGG 1 cut(s) 3139
BciVI GTATCC 1 cut(s) 3424
BclI TGATCA 3 cut(s) 232, 1507, 2308
BcoDI GTCTC 3 cut(s) 1170, 1590, 2149
BfaI CTAG 9 cut(s) 558, 1268, 1608, 1668, 1703, 1755, 2358, 2399, 2427
BfmI CTRYAG 1 cut(s) 3126
BfuAI ACCTGC 2 cut(s) 235, 1450
BfuI GTATCC 1 cut(s) 3424
BisI GCNGC 5 cut(s) 1032, 1557, 1914, 1917, 2190
BlsI GCNGC 5 cut(s) 1033, 1558, 1915, 1918, 2191
BmcAI AGTACT 2 cut(s) 838, 2164
Bme1390I CCNGG 1 cut(s) 3139
Bme18I GGWCC 1 cut(s) 695
BmgT120I GGNCC 1 cut(s) 695
BmiI GGNNCC 4 cut(s) 97, 128, 696, 1058
BmrFI CCNGG 1 cut(s) 3139
BmrI ACTGGG 2 cut(s) 2508, 2989
BmsI GCATC 7 cut(s) 377, 511, 534, 609, 1086, 1951, 2962
BmuI ACTGGG 2 cut(s) 2508, 2989
BpiI GAAGAC 2 cut(s) 147, 1682
BpmI CTGGAG 1 cut(s) 63
Bpu10I CCTNAGC 4 cut(s) 963, 1725, 2646, 3143
Bpu14I TTCGAA 1 cut(s) 3172
BpuEI CTTGAG 4 cut(s) 1154, 2082, 2616, 3232
Bsa29I ATCGAT 2 cut(s) 520, 1836
BsaBI GATNNNNATC 1 cut(s) 2736
BsaJI CCNNGG 2 cut(s) 1232, 1569
Bsc4I CCNNNNNNNGG 4 cut(s) 1066, 1570, 2399, 3144
Bse1I ACTGG 6 cut(s) 97, 2407, 2503, 2620, 2748, 2984
Bse3DI GCAATG 7 cut(s) 135, 184, 319, 2199, 2811, 2947, 3391
Bse8I GATNNNNATC 1 cut(s) 2736
BseBI CCWGG 1 cut(s) 3139
BseCI ATCGAT 2 cut(s) 520, 1836
BseDI CCNNGG 2 cut(s) 1232, 1569
BseGI GGATG 8 cut(s) 142, 343, 549, 1101, 1323, 1942, 2227, 2302
BseJI GATNNNNATC 1 cut(s) 2736
BseLI CCNNNNNNNGG 4 cut(s) 1066, 1570, 2399, 3144
BseMI GCAATG 7 cut(s) 135, 184, 319, 2199, 2811, 2947, 3391
BseNI ACTGG 6 cut(s) 97, 2407, 2503, 2620, 2748, 2984
BseRI GAGGAG 4 cut(s) 894, 951, 1459, 1967
BseXI GCAGC 5 cut(s) 1018, 1543, 1900, 1903, 2176
BseYI CCCAGC 1 cut(s) 1564
BsgI GTGCAG 4 cut(s) 377, 920, 3165, 3198
BshNI GGYRCC 1 cut(s) 95
BshVI ATCGAT 2 cut(s) 520, 1836
BsiHKAI GWGCWC 2 cut(s) 10, 2782
BslFI GGGAC 1 cut(s) 1979
BslI CCNNNNNNNGG 4 cut(s) 1066, 1570, 2399, 3144
BsmAI GTCTC 3 cut(s) 1170, 1590, 2149
BsmBI CGTCTC 1 cut(s) 1590
BsmFI GGGAC 1 cut(s) 1979
BsmI GAATGC 2 cut(s) 2443, 2816
Bsp119I TTCGAA 1 cut(s) 3172
Bsp1286I GDGCHC 2 cut(s) 10, 2782
Bsp1407I TGTACA 2 cut(s) 384, 2863
BspACI CCGC 1 cut(s) 1584
BspDI ATCGAT 2 cut(s) 520, 1836
BspHI TCATGA 2 cut(s) 1216, 1336
BspLI GGNNCC 4 cut(s) 97, 128, 696, 1058
BspMAI CTGCAG 1 cut(s) 3130
BspMI ACCTGC 2 cut(s) 235, 1450
BspPI GGATC 9 cut(s) 28, 43, 67, 484, 1223, 1880, 2071, 2417, 3079
BspT104I TTCGAA 1 cut(s) 3172
BspT107I GGYRCC 1 cut(s) 95
BsrDI GCAATG 7 cut(s) 135, 184, 319, 2199, 2811, 2947, 3391
BsrGI TGTACA 2 cut(s) 384, 2863
BsrI ACTGG 6 cut(s) 97, 2407, 2503, 2620, 2748, 2984
BssECI CCNNGG 2 cut(s) 1232, 1569
BssNAI GTATAC 2 cut(s) 1925, 2266
BssT1I CCWWGG 2 cut(s) 1232, 1569
Bst1107I GTATAC 2 cut(s) 1925, 2266
Bst2UI CCWGG 1 cut(s) 3139
Bst4CI ACNGT 5 cut(s) 384, 2167, 2833, 3041, 3381
Bst6I CTCTTC 4 cut(s) 419, 870, 1116, 1455
BstAUI TGTACA 2 cut(s) 384, 2863
BstBI TTCGAA 1 cut(s) 3172
BstC8I GCNNGC 2 cut(s) 2394, 2668
BstEII GGTNACC 1 cut(s) 627
BstENI CCTNNNNNAGG 1 cut(s) 3142
BstF5I GGATG 8 cut(s) 142, 343, 549, 1101, 1323, 1942, 2227, 2302
BstMAI GTCTC 3 cut(s) 1170, 1590, 2149
BstMWI GCNNNNNNNGC 5 cut(s) 374, 1565, 2189, 2360, 2981
BstNI CCWGG 1 cut(s) 3139
BstNSI RCATGY 8 cut(s) 51, 266, 951, 1014, 1331, 2682, 2714, 3152
BstPI GGTNACC 1 cut(s) 627
BstSCI CCNGG 1 cut(s) 3137
BstSFI CTRYAG 1 cut(s) 3126
BstV1I GCAGC 5 cut(s) 1018, 1543, 1900, 1903, 2176
BstV2I GAAGAC 2 cut(s) 147, 1682
BstX2I RGATCY 4 cut(s) 35, 59, 1885, 3071
BstYI RGATCY 4 cut(s) 35, 59, 1885, 3071
BstZ17I GTATAC 2 cut(s) 1925, 2266
Bsu15I ATCGAT 2 cut(s) 520, 1836
BsuI GTATCC 1 cut(s) 3424
BsuTUI ATCGAT 2 cut(s) 520, 1836
BtsCI GGATG 8 cut(s) 142, 343, 549, 1101, 1323, 1942, 2227, 2302
BtsI GCAGTG 1 cut(s) 462
BtsIMutI CAGTG 4 cut(s) 462, 2741, 3037, 3377
BveI ACCTGC 2 cut(s) 235, 1450
Cac8I GCNNGC 2 cut(s) 2394, 2668
CaiI CAGNNNCTG 1 cut(s) 3098
CciI TCATGA 2 cut(s) 1216, 1336
Cfr13I GGNCC 1 cut(s) 695
ClaI ATCGAT 2 cut(s) 520, 1836
CseI GACGC 1 cut(s) 1607
Csp6I GTAC 8 cut(s) 385, 837, 1117, 1182, 1980, 2163, 2273, 2864
CviQI GTAC 8 cut(s) 385, 837, 1117, 1182, 1980, 2163, 2273, 2864
DraIII CACNNNGTG 1 cut(s) 1382
DrdI GACNNNNNNGTC 1 cut(s) 2489
DseDI GACNNNNNNGTC 1 cut(s) 2489
Eam1104I CTCTTC 4 cut(s) 419, 870, 1116, 1455
EarI CTCTTC 4 cut(s) 419, 870, 1116, 1455
Eco130I CCWWGG 2 cut(s) 1232, 1569
Eco32I GATATC 1 cut(s) 3352
Eco47I GGWCC 1 cut(s) 695
Eco57I CTGAAG 1 cut(s) 3302
Eco91I GGTNACC 1 cut(s) 627
EcoNI CCTNNNNNAGG 1 cut(s) 3142
EcoO65I GGTNACC 1 cut(s) 627
EcoRI GAATTC 2 cut(s) 2619, 3174
EcoRII CCWGG 1 cut(s) 3137
EcoRV GATATC 1 cut(s) 3352
EcoT14I CCWWGG 2 cut(s) 1232, 1569
EcoT22I ATGCAT 2 cut(s) 624, 1016
ErhI CCWWGG 2 cut(s) 1232, 1569
Esp3I CGTCTC 1 cut(s) 1590
FalI AAGNNNNNCTT 6 cut(s) 1509, 1541, 2073, 2105, 2533, 2565
FaqI GGGAC 1 cut(s) 1979
FauNDI CATATG 2 cut(s) 108, 591
FbaI TGATCA 3 cut(s) 232, 1507, 2308
FblI GTMKAC 5 cut(s) 491, 1324, 1924, 2265, 2481
Fnu4HI GCNGC 5 cut(s) 1032, 1557, 1914, 1917, 2190
FokI GGATG 8 cut(s) 129, 350, 556, 1108, 1330, 1949, 2234, 2289
Fsp4HI GCNGC 5 cut(s) 1032, 1557, 1914, 1917, 2190
FspBI CTAG 9 cut(s) 558, 1268, 1608, 1668, 1703, 1755, 2358, 2399, 2427
GluI GCNGC 5 cut(s) 1032, 1557, 1914, 1917, 2190
GsaI CCCAGC 1 cut(s) 1568
GsuI CTGGAG 1 cut(s) 63
HgaI GACGC 1 cut(s) 1607
HincII GTYRAC 5 cut(s) 1325, 1752, 2293, 2850, 2995
HindII GTYRAC 5 cut(s) 1325, 1752, 2293, 2850, 2995
HindIII AAGCTT 2 cut(s) 245, 2547
HpaI GTTAAC 2 cut(s) 1752, 2995
Hpy188I TCNGA 9 cut(s) 118, 640, 796, 1507, 1618, 1627, 2449, 2506, 3300
HpyCH4III ACNGT 5 cut(s) 384, 2167, 2833, 3041, 3381
HpyF10VI GCNNNNNNNGC 5 cut(s) 374, 1565, 2189, 2360, 2981
Ksp22I TGATCA 3 cut(s) 232, 1507, 2308
KspAI GTTAAC 2 cut(s) 1752, 2995
LmnI GCTCC 8 cut(s) 44, 706, 1396, 2025, 2359, 2766, 2777, 3401
Lsp1109I GCAGC 5 cut(s) 1018, 1543, 1900, 1903, 2176
LweI GCATC 7 cut(s) 377, 511, 534, 609, 1086, 1951, 2962
MaeI CTAG 9 cut(s) 558, 1268, 1608, 1668, 1703, 1755, 2358, 2399, 2427
MaeIII GTNAC 4 cut(s) 258, 627, 736, 1512
MfeI CAATTG 1 cut(s) 2216
MflI RGATCY 4 cut(s) 35, 59, 1885, 3071
MhlI GDGCHC 2 cut(s) 10, 2782
MlyI GAGTC 4 cut(s) 161, 405, 650, 2288
MmeI TCCRAC 2 cut(s) 2755, 3353
Mph1103I ATGCAT 2 cut(s) 624, 1016
MroXI GAANNNNTTC 1 cut(s) 3294
MslI CAYNNNNRTG 1 cut(s) 594
MspA1I CMGCKG 1 cut(s) 482
MspR9I CCNGG 1 cut(s) 3139
MunI CAATTG 1 cut(s) 2216
Mva1269I GAATGC 2 cut(s) 2443, 2816
MvaI CCWGG 1 cut(s) 3139
MwoI GCNNNNNNNGC 5 cut(s) 374, 1565, 2189, 2360, 2981
NdeI CATATG 2 cut(s) 108, 591
NlaIV GGNNCC 4 cut(s) 97, 128, 696, 1058
NsiI ATGCAT 2 cut(s) 624, 1016
NspI RCATGY 8 cut(s) 51, 266, 951, 1014, 1331, 2682, 2714, 3152
NspV TTCGAA 1 cut(s) 3172
PagI TCATGA 2 cut(s) 1216, 1336
PaqCI CACCTGC 2 cut(s) 235, 1450
PciI ACATGT 5 cut(s) 262, 947, 1327, 2678, 2710
PctI GAATGC 2 cut(s) 2443, 2816
PdmI GAANNNNTTC 1 cut(s) 3294
PfeI GAWTC 8 cut(s) 931, 1333, 1343, 1619, 1628, 2403, 2592, 2887
PflFI GACNNNGTC 1 cut(s) 513
PflMI CCANNNNNTGG 1 cut(s) 1066
PkrI GCNGC 5 cut(s) 1033, 1558, 1915, 1918, 2191
PleI GAGTC 4 cut(s) 160, 405, 649, 2288
PpsI GAGTC 4 cut(s) 160, 405, 649, 2288
PscI ACATGT 5 cut(s) 262, 947, 1327, 2678, 2710
PshBI ATTAAT 1 cut(s) 2958
PsiI TTATAA 1 cut(s) 450
Psp6I CCWGG 1 cut(s) 3137
PspEI GGTNACC 1 cut(s) 627
PspFI CCCAGC 1 cut(s) 1564
PspGI CCWGG 1 cut(s) 3137
PspN4I GGNNCC 4 cut(s) 97, 128, 696, 1058
PspPI GGNCC 1 cut(s) 695
PstI CTGCAG 1 cut(s) 3130
PstNI CAGNNNCTG 1 cut(s) 3098
PsuI RGATCY 4 cut(s) 35, 59, 1885, 3071
PsyI GACNNNGTC 1 cut(s) 513
PvuII CAGCTG 1 cut(s) 482
RsaI GTAC 8 cut(s) 386, 838, 1118, 1183, 1981, 2164, 2274, 2865
RsaNI GTAC 8 cut(s) 385, 837, 1117, 1182, 1980, 2163, 2273, 2864
RseI CAYNNNNRTG 1 cut(s) 594
SalI GTCGAC 1 cut(s) 1323
SatI GCNGC 5 cut(s) 1032, 1557, 1914, 1917, 2190
Sau96I GGNCC 1 cut(s) 695
ScaI AGTACT 2 cut(s) 838, 2164
SchI GAGTC 4 cut(s) 161, 405, 650, 2288
ScrFI CCNGG 1 cut(s) 3139
SduI GDGCHC 2 cut(s) 10, 2782
SfaNI GCATC 7 cut(s) 377, 511, 534, 609, 1086, 1951, 2962
SfcI CTRYAG 1 cut(s) 3126
SfuI TTCGAA 1 cut(s) 3172
SinI GGWCC 1 cut(s) 695
SmiMI CAYNNNNRTG 1 cut(s) 594
SmlI CTYRAG 4 cut(s) 1169, 2097, 2631, 3247
SmoI CTYRAG 4 cut(s) 1169, 2097, 2631, 3247
SsiI CCGC 1 cut(s) 1584
SspMI CTAG 9 cut(s) 558, 1268, 1608, 1668, 1703, 1755, 2358, 2399, 2427
StyD4I CCNGG 1 cut(s) 3137
StyI CCWWGG 2 cut(s) 1232, 1569
TaaI ACNGT 5 cut(s) 384, 2167, 2833, 3041, 3381
TaqI TCGA 5 cut(s) 164, 520, 1324, 1836, 3172
TatI WGTACW 4 cut(s) 384, 836, 2162, 2863
TfiI GAWTC 8 cut(s) 931, 1333, 1343, 1619, 1628, 2403, 2592, 2887
TscAI CASTG 4 cut(s) 469, 2748, 3044, 3384
TseI GCWGC 5 cut(s) 1031, 1556, 1913, 1916, 2189
TspRI CASTG 4 cut(s) 469, 2748, 3044, 3384
Tth111I GACNNNGTC 1 cut(s) 513
Van91I CCANNNNNTGG 1 cut(s) 1066
VpaK11BI GGWCC 1 cut(s) 695
VspI ATTAAT 1 cut(s) 2958
XagI CCTNNNNNAGG 1 cut(s) 3142
XapI RAATTY 4 cut(s) 675, 761, 2619, 3174
XceI RCATGY 8 cut(s) 51, 266, 951, 1014, 1331, 2682, 2714, 3152
XcmI CCANNNNNNNNNTGG 1 cut(s) 3406
XmiI GTMKAC 5 cut(s) 491, 1324, 1924, 2265, 2481
XmnI GAANNNNTTC 1 cut(s) 3294
XspI CTAG 9 cut(s) 558, 1268, 1608, 1668, 1703, 1755, 2358, 2399, 2427
ZrmI AGTACT 2 cut(s) 838, 2164
Zsp2I ATGCAT 2 cut(s) 624, 1016
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.