RchiOBHm_Chr3g0482011
RLK Family

cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0001471
Physical Location & Seq
Reverse (-)
28106176 .. 28107678
1503 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ44691

Sequence Viewer

Length: 1185 bp
ATGTCTCAGCCGCAAGGTTTTGTTGATCCTCAATATCCCAATCATGTCTGCTTACTACGAAAATCTCTTTATGGTTTGAAACAAGCTCCAAGAGCTTGGAATGAGAAATTTACCAGTTTTCTACCAAGCTTAGGTTTTCAGTTTTCTCACTCTGATCCAAGCTTGTTTGTTAGACATTCATCTGCAGGACTGGTTGCATTATTACTATATGTGGATGATATAGTCATTACTGGTTCAGATACTGCTGGTATTCATGAAGTCATCTCTGAGTTAAGCATGGTGTTTGACATGAAGGATCTAGGTTCATTGTCATATTTCCTTGGTTTAGAAGTCACCAAAGTTCAACAAGGCATCTTACTTAGTCAATCTAAGTATGCTAGAGATTTGCTTGCTAAAACAGGAATGGAGTCTGTTAGGTCTTGCACCTCACCTTGTCTTCCCCACTCTCAGATGACATCAAATCAAGGTACTCTTCTTAAGGACCCCACTATGTACAGGAGTATAGTAGGAGCTTTACAATACTTAACTTTCACTCGTCCAGACATAGCATATGCAGTTAATACTGTATGTCAGTTCATGACAAGCCCTACAGATATACATTATGCTGCTGTTAAACGAATTTTAAGATACTTGAAGGGAACACTTGATAAGGGTATCTTTTATCGTTCTTGTGGTGCTTTGCATGATGCTGTGAATATCAAGGCATTTTGTGATGCTGATTGGGCTGGAGAAGTGATACAGAAACGATCTACTACTGGCTTTGTTGTCTATCTTGGACTATGTCCTGTGTCTTGGCAGTCTAAGAAACAAGGTAGTGTCTCCAGAAGTTCTACTGAGTCAGAGTATAGGAGTCTGGCCAATACTGCAGCTGAAATTAGTTGGCTTAGACATCTTCTGTGTGATTTGAAGATTCGAGTTCCTTGTGCTCCTTTGCTTAAGTGTGACAACTTGTCTGCTCTAGCATTGGCATCCAATCCAGTGTTCCATTCTAAGATCAAGCATTTGGACAATGATTTTCATTTTGTCCGTGAACGAGTTCAACACAATGATCTTCGTCTTGAGTATGTTTCCACTACAGAACAAACTGCAGATGTACTGACAAAGGGGTTAGCCTCACCTATATTTCGAACTCACTGTGTCAATCTCAGACTAGTCACCCTAGCTGAGCTTGAGGGAGGGTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2 Retrotran_gag_3 Retrotrans_gag gag_pre-integrs rve

Protein Analysis

394

Amino Acids

43.83

Weight (kDa)

8.52

Isoelectric Point (pI)

45.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_2 PF07727 1 - 146 1e-33 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 11
AclWI GGATC 3 cut(s) 20, 149, 303
AcoI YGGCCR 1 cut(s) 855
AcsI RAATTY 2 cut(s) 107, 618
AfaI GTAC 3 cut(s) 469, 494, 1095
AfiI CCNNNNNNNGG 1 cut(s) 131
AflII CTTAAG 2 cut(s) 476, 935
AgsI TTSAA 5 cut(s) 79, 344, 634, 907, 1040
AhdI GACNNNNNGTC 1 cut(s) 949
AhlI ACTAGT 1 cut(s) 1150
AjuI GAANNNNNNNTTGG 2 cut(s) 965, 997
AluBI AGCT 8 cut(s) 86, 95, 129, 162, 512, 869, 1163, 1168
AluI AGCT 8 cut(s) 86, 95, 129, 162, 512, 869, 1163, 1168
Alw21I GWGCWC 1 cut(s) 928
Alw26I GTCTC 2 cut(s) 9, 823
AlwI GGATC 3 cut(s) 20, 149, 303
AlwNI CAGNNNCTG 1 cut(s) 242
AoxI GGCC 1 cut(s) 855
ApeKI GCWGC 2 cut(s) 605, 866
ApoI RAATTY 2 cut(s) 107, 618
Asp700I GAANNNNTTC 1 cut(s) 1035
AspS9I GGNCC 1 cut(s) 481
AsuHPI GGTGA 4 cut(s) 325, 420, 1107, 1147
AsuII TTCGAA 1 cut(s) 1126
AvaII GGWCC 1 cut(s) 481
BalI TGGCCA 1 cut(s) 857
BbsI GAAGAC 1 cut(s) 428
Bbv12I GWGCWC 1 cut(s) 928
BbvI GCAGC 2 cut(s) 592, 878
BcoDI GTCTC 2 cut(s) 9, 823
BcuI ACTAGT 1 cut(s) 1150
BfaI CTAG 5 cut(s) 299, 378, 959, 1151, 1160
BfmI CTRYAG 5 cut(s) 183, 588, 864, 1074, 1086
BfrI CTTAAG 2 cut(s) 476, 935
BisI GCNGC 3 cut(s) 11, 606, 867
BlpI GCTNAGC 1 cut(s) 1164
BlsI GCNGC 3 cut(s) 12, 607, 868
Bme18I GGWCC 1 cut(s) 481
BmeRI GACNNNNNGTC 1 cut(s) 949
BmgT120I GGNCC 1 cut(s) 481
BmiI GGNNCC 1 cut(s) 483
BmsI GCATC 4 cut(s) 360, 676, 703, 977
BpiI GAAGAC 1 cut(s) 428
BpmI CTGGAG 2 cut(s) 747, 805
Bpu10I CCTNAGC 1 cut(s) 130
Bpu1102I GCTNAGC 1 cut(s) 1164
Bpu14I TTCGAA 1 cut(s) 1126
BpuEI CTTGAG 1 cut(s) 1079
BsaJI CCNNGG 1 cut(s) 319
Bsc4I CCNNNNNNNGG 1 cut(s) 131
Bse1I ACTGG 5 cut(s) 114, 195, 235, 760, 977
BseDI CCNNGG 1 cut(s) 319
BseGI GGATG 2 cut(s) 220, 968
BseLI CCNNNNNNNGG 1 cut(s) 131
BseMII CTCAG 6 cut(s) 20, 258, 461, 825, 1155, 1159
BseNI ACTGG 5 cut(s) 114, 195, 235, 760, 977
BseXI GCAGC 2 cut(s) 592, 878
BshFI GGCC 1 cut(s) 857
BsiHKAI GWGCWC 1 cut(s) 928
BslI CCNNNNNNNGG 1 cut(s) 131
BsmAI GTCTC 2 cut(s) 9, 823
BsnI GGCC 1 cut(s) 857
Bsp119I TTCGAA 1 cut(s) 1126
Bsp1286I GDGCHC 1 cut(s) 928
Bsp1407I TGTACA 1 cut(s) 492
Bsp143I GATC 6 cut(s) 25, 154, 295, 746, 993, 1048
Bsp1720I GCTNAGC 1 cut(s) 1164
BspACI CCGC 1 cut(s) 11
BspANI GGCC 1 cut(s) 857
BspCNI CTCAG 6 cut(s) 19, 259, 460, 826, 1156, 1158
BspHI TCATGA 2 cut(s) 253, 576
BspLI GGNNCC 1 cut(s) 483
BspMAI CTGCAG 3 cut(s) 187, 868, 1090
BspPI GGATC 3 cut(s) 20, 149, 303
BspT104I TTCGAA 1 cut(s) 1126
BspTI CTTAAG 2 cut(s) 476, 935
BsrGI TGTACA 1 cut(s) 492
BsrI ACTGG 5 cut(s) 114, 195, 235, 760, 977
BssECI CCNNGG 1 cut(s) 319
BssMI GATC 6 cut(s) 25, 154, 295, 746, 993, 1048
BssT1I CCWWGG 1 cut(s) 319
Bst4CI ACNGT 2 cut(s) 565, 1136
Bst6I CTCTTC 1 cut(s) 477
BstAFI CTTAAG 2 cut(s) 476, 935
BstAUI TGTACA 1 cut(s) 492
BstBI TTCGAA 1 cut(s) 1126
BstC8I GCNNGC 1 cut(s) 390
BstF5I GGATG 2 cut(s) 220, 968
BstKTI GATC 6 cut(s) 28, 157, 298, 749, 996, 1051
BstMAI GTCTC 2 cut(s) 9, 823
BstMBI GATC 6 cut(s) 25, 154, 295, 746, 993, 1048
BstMWI GCNNNNNNNGC 3 cut(s) 92, 722, 863
BstSFI CTRYAG 5 cut(s) 183, 588, 864, 1074, 1086
BstV1I GCAGC 2 cut(s) 592, 878
BstV2I GAAGAC 1 cut(s) 428
BstX2I RGATCY 1 cut(s) 295
BstXI CCANNNNNNTGG 1 cut(s) 96
BstYI RGATCY 1 cut(s) 295
BsuRI GGCC 1 cut(s) 857
BtsCI GGATG 2 cut(s) 220, 968
BtsIMutI CAGTG 2 cut(s) 984, 1132
Cac8I GCNNGC 1 cut(s) 390
CaiI CAGNNNCTG 1 cut(s) 242
CciI TCATGA 2 cut(s) 253, 576
Cfr13I GGNCC 1 cut(s) 481
Csp6I GTAC 3 cut(s) 468, 493, 1094
CviAII CATG 6 cut(s) 44, 254, 277, 289, 577, 683
CviQI GTAC 3 cut(s) 468, 493, 1094
DpnI GATC 6 cut(s) 27, 156, 297, 748, 995, 1050
DpnII GATC 6 cut(s) 25, 154, 295, 746, 993, 1048
DriI GACNNNNNGTC 1 cut(s) 949
EaeI YGGCCR 1 cut(s) 855
Eam1104I CTCTTC 1 cut(s) 477
Eam1105I GACNNNNNGTC 1 cut(s) 949
EarI CTCTTC 1 cut(s) 477
Eco130I CCWWGG 1 cut(s) 319
Eco47I GGWCC 1 cut(s) 481
EcoO109I RGGNCCY 1 cut(s) 481
EcoT14I CCWWGG 1 cut(s) 319
ErhI CCWWGG 1 cut(s) 319
FaeI CATG 6 cut(s) 47, 257, 280, 292, 580, 686
FalI AAGNNNNNCTT 4 cut(s) 456, 488, 641, 673
FatI CATG 6 cut(s) 43, 253, 276, 288, 576, 682
FauNDI CATATG 1 cut(s) 550
Fnu4HI GCNGC 3 cut(s) 11, 606, 867
FokI GGATG 2 cut(s) 227, 955
Fsp4HI GCNGC 3 cut(s) 11, 606, 867
FspBI CTAG 5 cut(s) 299, 378, 959, 1151, 1160
GluI GCNGC 3 cut(s) 11, 606, 867
GsuI CTGGAG 2 cut(s) 747, 805
HaeIII GGCC 1 cut(s) 857
Hin1II CATG 6 cut(s) 47, 257, 280, 292, 580, 686
HindIII AAGCTT 2 cut(s) 127, 160
HinfI GANTC 4 cut(s) 407, 836, 850, 910
HphI GGTGA 4 cut(s) 325, 420, 1107, 1147
Hpy166II GTNNAC 1 cut(s) 1031
Hpy188I TCNGA 6 cut(s) 154, 238, 268, 450, 841, 1148
Hpy188III TCNNGA 5 cut(s) 254, 539, 577, 822, 1058
Hpy8I GTNNAC 1 cut(s) 1031
HpyAV CCTTC 2 cut(s) 286, 628
HpyCH4III ACNGT 2 cut(s) 565, 1136
HpyCH4V TGCA 7 cut(s) 185, 197, 423, 554, 682, 866, 1088
HpyF10VI GCNNNNNNNGC 3 cut(s) 92, 722, 863
Hsp92II CATG 6 cut(s) 47, 257, 280, 292, 580, 686
Kzo9I GATC 6 cut(s) 25, 154, 295, 746, 993, 1048
LmnI GCTCC 3 cut(s) 91, 509, 931
Lsp1109I GCAGC 2 cut(s) 592, 878
LweI GCATC 4 cut(s) 360, 676, 703, 977
MaeI CTAG 5 cut(s) 299, 378, 959, 1151, 1160
MaeIII GTNAC 3 cut(s) 331, 941, 1153
MalI GATC 6 cut(s) 27, 156, 297, 748, 995, 1050
MboI GATC 6 cut(s) 25, 154, 295, 746, 993, 1048
MboII GAAGA 5 cut(s) 428, 464, 884, 919, 1043
MflI RGATCY 1 cut(s) 295
MhlI GDGCHC 1 cut(s) 928
MlsI TGGCCA 1 cut(s) 857
MluCI AATT 3 cut(s) 107, 618, 873
MluNI TGGCCA 1 cut(s) 857
MlyI GAGTC 3 cut(s) 416, 845, 859
MnlI CCTC 5 cut(s) 39, 436, 1123, 1165, 1169
Mox20I TGGCCA 1 cut(s) 857
MroXI GAANNNNTTC 1 cut(s) 1035
MscI TGGCCA 1 cut(s) 857
MseI TTAA 7 cut(s) 272, 477, 524, 558, 612, 623, 936
Msp20I TGGCCA 1 cut(s) 857
MspA1I CMGCKG 1 cut(s) 869
MspCI CTTAAG 2 cut(s) 476, 935
MwoI GCNNNNNNNGC 3 cut(s) 92, 722, 863
NdeI CATATG 1 cut(s) 550
NdeII GATC 6 cut(s) 25, 154, 295, 746, 993, 1048
NlaIII CATG 6 cut(s) 47, 257, 280, 292, 580, 686
NlaIV GGNNCC 1 cut(s) 483
NmuCI GTSAC 3 cut(s) 331, 941, 1153
NspV TTCGAA 1 cut(s) 1126
PagI TCATGA 2 cut(s) 253, 576
PdmI GAANNNNTTC 1 cut(s) 1035
PfeI GAWTC 1 cut(s) 910
PflFI GACNNNGTC 1 cut(s) 780
PkrI GCNGC 3 cut(s) 12, 607, 868
PleI GAGTC 3 cut(s) 415, 844, 858
PpsI GAGTC 3 cut(s) 415, 844, 858
PpuMI RGGWCCY 1 cut(s) 481
Psp5II RGGWCCY 1 cut(s) 481
PspN4I GGNNCC 1 cut(s) 483
PspPI GGNCC 1 cut(s) 481
PspPPI RGGWCCY 1 cut(s) 481
PstI CTGCAG 3 cut(s) 187, 868, 1090
PstNI CAGNNNCTG 1 cut(s) 242
PsuI RGATCY 1 cut(s) 295
PsyI GACNNNGTC 1 cut(s) 780
PvuII CAGCTG 1 cut(s) 869
RsaI GTAC 3 cut(s) 469, 494, 1095
RsaNI GTAC 3 cut(s) 468, 493, 1094
SaqAI TTAA 7 cut(s) 272, 477, 524, 558, 612, 623, 936
SatI GCNGC 3 cut(s) 11, 606, 867
Sau3AI GATC 6 cut(s) 25, 154, 295, 746, 993, 1048
Sau96I GGNCC 1 cut(s) 481
SchI GAGTC 3 cut(s) 416, 845, 859
SduI GDGCHC 1 cut(s) 928
SfaNI GCATC 4 cut(s) 360, 676, 703, 977
SfcI CTRYAG 5 cut(s) 183, 588, 864, 1074, 1086
SfuI TTCGAA 1 cut(s) 1126
SinI GGWCC 1 cut(s) 481
SmlI CTYRAG 4 cut(s) 476, 935, 1058, 1169
SmoI CTYRAG 4 cut(s) 476, 935, 1058, 1169
SpeI ACTAGT 1 cut(s) 1150
Sse9I AATT 3 cut(s) 107, 618, 873
SsiI CCGC 1 cut(s) 11
SspMI CTAG 5 cut(s) 299, 378, 959, 1151, 1160
StyI CCWWGG 1 cut(s) 319
TaaI ACNGT 2 cut(s) 565, 1136
TaqI TCGA 2 cut(s) 913, 1126
TasI AATT 3 cut(s) 107, 618, 873
TatI WGTACW 2 cut(s) 492, 1093
TauI GCSGC 1 cut(s) 13
TfiI GAWTC 1 cut(s) 910
Tru1I TTAA 7 cut(s) 272, 477, 524, 558, 612, 623, 936
Tru9I TTAA 7 cut(s) 272, 477, 524, 558, 612, 623, 936
TscAI CASTG 2 cut(s) 984, 1139
TseFI GTSAC 3 cut(s) 331, 941, 1153
TseI GCWGC 2 cut(s) 605, 866
Tsp45I GTSAC 3 cut(s) 331, 941, 1153
TspDTI ATGAA 7 cut(s) 168, 242, 270, 294, 305, 565, 1007
TspGWI ACGGA 1 cut(s) 1016
TspRI CASTG 2 cut(s) 984, 1139
Tth111I GACNNNGTC 1 cut(s) 780
Vha464I CTTAAG 2 cut(s) 476, 935
VpaK11BI GGWCC 1 cut(s) 481
XapI RAATTY 2 cut(s) 107, 618
XmnI GAANNNNTTC 1 cut(s) 1035
XspI CTAG 5 cut(s) 299, 378, 959, 1151, 1160
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.