RchiOBHm_Chr7g0200081
RLK Family

cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0001471
Physical Location & Seq
Reverse (-)
17987974 .. 17989844
1871 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ17907

Sequence Viewer

Length: 1758 bp
ATGACAACTAGAAGCAAGAATGGAATATTCAAAAAGAAGGCATTTGTTGCTTCTGCAGCTTGTGATCTCAATGCTATAGAACCTTCTTCATATAAAAAGGCCATGGAGATACCAGTTTGGAAGCAAGCAATGCAAGAAGAATTTGATGCATTAACCAACCAAGGTACTTGGAGTTTAGTTCCTCCTCAAGCTGATAAGAATGTGGTAAGCTGTAAATGGATTTTTAAGGTAAAGAAAAATTCAGATGGCTCCATTTCAAGGCAGAAAGCACGATTGGTAGCAAGGGGTTTCAGTCAAGAAGAAGGTATTGATTATGACGAGACTTTCAGTCCAGTTGTTAGGCATACAACTGTGAGACTTGTTCTTGCTATGGCAGCTCAGTTTGGGTGGAAATTACATCAACTAGATGTTAAGAATACTTTTCTTCATGGCATTTTGAAGGAAGAAGTATATATGTCCCAACCACCTGGTTTTGAGGATCCAAGGTGTCCAACTCATGTTTGCAAGTTACAAAAGTCACTTTATGGTCTTAAACAAGCTCCAAGAGCGTGGAATGAAAGATTCACCTCTTTCTTACCTCAGTTGGGTTTCAAGTGTTCTATTGCAGACCCCTCTTTATTCATTAAGAGTTCTGGTCCCTCTCAGGTATATCTACTCCTATATGTAGATGATATTATACTTACAGGCAATAGTGCTTCTGCAATTCAGTATGTTAAAGATGCTCTTAAGAAGGAATTTGAAATGAAGGATTTGGGACTATTACATTACTTCTTGGGATTACAGATTACCTATTTACTAGATGGAGGTTTATTCATTTCGCAAGCTAAGTATGCAAAGGATATTCTTGATAAAGCTGGAATGACTGATTGTAATGCCAGCATCACTCCATGCTTACCTTATTCCAAGTTGCTCAAAGATGAGGGAGCTCCATTTAAAGATGTCAAAACGTACAGGAGTATTGTAGGATGCTTACAGTATCTAACTTTCACCAGACCTGACATTGCATATAGTGTCAATTCTGTGTGCCAATTTATGCAAAATCCAACTAAAGTACATTTTCTGGCAGTCAAGCGCATTCTAAGGTACATCAAAGGTACTCTGAACTATGGTATTACTTTTAGAGCTGCACCATTGGAGTTAAGAGCCTATACAGACTCGGATTGGGCTGGTGACCCTAATGATAGAAGGAGCACAACAGGATTTGTCATATTTCTTGGTAACTGTCCAATCTCTTGGAGCTCACGCAAGCAAACTTCAGTCTCAAGGTCTTCAACAGAGGCCGAGTACAGAGCTATGGCTGACACTGCTTCAGAAGTTCTTTGGCTGCGACATTTGTTGAATGATCTTCATGTCAAGTTACCTTCTGCTCCTACATTACACTGTGACAATGTTTCAGCCTTGGCCCTTGCTTCGAATCCAATTCATAATTCCAAGGTCAAGCATGTGGAAGTGGATGTGCATTTCACTAGGGAAAAAGTGGCCAGAGGTGAGCTAGCTCTTCAGTTTGTTCCTTCCTTGCAACAGCTAGCAGATATCTTGACAAAAGGACTTGATTCTCCTCAATTCAAATATCTCTGCTCCAACCTGATGCTTGGTTCCCTGCATCAGTTTGAGGGGGGATGTAAGGATTATAGTGATTGTCAAGATGGTCAAGGTTCACAAGTCTTACGAGTAAGCAAGGCAACAGATGTGGCTACTAAGGCTGACACGTGTGATGATAAGACTGGTTATCAAGAATTAGTTACTATAACTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2 Retrotran_gag_3 Retrotrans_gag gag_pre-integrs rve

Protein Analysis

585

Amino Acids

65.41

Weight (kDa)

8.75

Isoelectric Point (pI)

37.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_2 PF07727 56 - 298 1.7e-74 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 473, 486
AcoI YGGCCR 1 cut(s) 1479
AcsI RAATTY 3 cut(s) 140, 238, 734
AcuI CTGAAG 3 cut(s) 1239, 1293, 1484
AcvI CACGTG 1 cut(s) 1710
AfaI GTAC 6 cut(s) 166, 950, 1053, 1085, 1096, 1286
AfiI CCNNNNNNNGG 2 cut(s) 258, 584
AflII CTTAAG 1 cut(s) 725
AflIII ACRYGT 2 cut(s) 1707, 1709
AgsI TTSAA 8 cut(s) 31, 258, 439, 592, 740, 1272, 1339, 1567
AhdI GACNNNNNGTC 1 cut(s) 327
AjnI CCWGG 1 cut(s) 466
AjuI GAANNNNNNNTTGG 4 cut(s) 257, 289, 1411, 1443
Alw21I GWGCWC 3 cut(s) 928, 1193, 1241
Alw26I GTCTC 3 cut(s) 314, 349, 1264
AlwI GGATC 2 cut(s) 473, 486
AoxI GGCC 4 cut(s) 99, 1278, 1401, 1479
ApeKI GCWGC 4 cut(s) 56, 374, 1124, 1324
ApoI RAATTY 3 cut(s) 140, 238, 734
AspLEI GCGC 1 cut(s) 1074
AspS9I GGNCC 2 cut(s) 635, 1402
AsuHPI GGTGA 4 cut(s) 556, 979, 1181, 1499
AsuII TTCGAA 1 cut(s) 1412
AsuNHI GCTAGC 2 cut(s) 1492, 1525
AvaII GGWCC 1 cut(s) 635
BalI TGGCCA 1 cut(s) 1481
BamHI GGATCC 1 cut(s) 478
BanII GRGCYC 2 cut(s) 928, 1241
BbrPI CACGTG 1 cut(s) 1710
BbsI GAAGAC 1 cut(s) 1260
Bbv12I GWGCWC 3 cut(s) 928, 1193, 1241
BbvI GCAGC 4 cut(s) 68, 386, 1111, 1311
BccI CCATC 3 cut(s) 239, 794, 1640
BciT130I CCWGG 1 cut(s) 468
BcoDI GTCTC 3 cut(s) 314, 349, 1264
BfaI CTAG 6 cut(s) 9, 404, 797, 1467, 1493, 1526
BfmI CTRYAG 2 cut(s) 54, 75
BfrI CTTAAG 1 cut(s) 725
BisI GCNGC 4 cut(s) 57, 375, 1125, 1325
BlsI GCNGC 4 cut(s) 58, 376, 1126, 1326
Bme1390I CCNGG 1 cut(s) 468
Bme18I GGWCC 1 cut(s) 635
BmeRI GACNNNNNGTC 1 cut(s) 327
BmgT120I GGNCC 2 cut(s) 635, 1402
BmiI GGNNCC 4 cut(s) 250, 480, 637, 1597
BmrFI CCNGG 1 cut(s) 468
BmsI GCATC 6 cut(s) 136, 709, 888, 956, 1578, 1612
BmtI GCTAGC 2 cut(s) 1496, 1529
BpiI GAAGAC 1 cut(s) 1260
Bpu14I TTCGAA 1 cut(s) 1412
BpuEI CTTGAG 2 cut(s) 171, 1246
BsaAI YACGTR 1 cut(s) 1710
BsaJI CCNNGG 5 cut(s) 102, 160, 482, 1398, 1431
Bsc4I CCNNNNNNNGG 2 cut(s) 258, 584
Bse1I ACTGG 3 cut(s) 113, 332, 1729
Bse3DI GCAATG 2 cut(s) 135, 999
BseBI CCWGG 1 cut(s) 468
BseDI CCNNGG 5 cut(s) 102, 160, 482, 1398, 1431
BseGI GGATG 3 cut(s) 971, 1459, 1625
BseLI CCNNNNNNNGG 2 cut(s) 258, 584
BseMI GCAATG 2 cut(s) 135, 999
BseMII CTCAG 3 cut(s) 392, 593, 656
BseNI ACTGG 3 cut(s) 113, 332, 1729
BseRI GAGGAG 2 cut(s) 174, 1548
BseXI GCAGC 4 cut(s) 68, 386, 1111, 1311
BsgI GTGCAG 1 cut(s) 1110
BshFI GGCC 4 cut(s) 101, 1280, 1403, 1481
BsiHKAI GWGCWC 3 cut(s) 928, 1193, 1241
BslFI GGGAC 3 cut(s) 442, 621, 768
BslI CCNNNNNNNGG 2 cut(s) 258, 584
BsmAI GTCTC 3 cut(s) 314, 349, 1264
BsmFI GGGAC 3 cut(s) 442, 621, 768
BsmI GAATGC 1 cut(s) 1074
BsnI GGCC 4 cut(s) 101, 1280, 1403, 1481
Bsp119I TTCGAA 1 cut(s) 1412
Bsp1286I GDGCHC 3 cut(s) 928, 1193, 1241
Bsp143I GATC 3 cut(s) 64, 478, 1342
Bsp19I CCATGG 1 cut(s) 102
BspANI GGCC 4 cut(s) 101, 1280, 1403, 1481
BspCNI CTCAG 3 cut(s) 391, 592, 655
BspLI GGNNCC 4 cut(s) 250, 480, 637, 1597
BspMAI CTGCAG 1 cut(s) 58
BspOI GCTAGC 2 cut(s) 1496, 1529
BspPI GGATC 2 cut(s) 473, 486
BspQI GCTCTTC 1 cut(s) 1503
BspT104I TTCGAA 1 cut(s) 1412
BspTI CTTAAG 1 cut(s) 725
BsrDI GCAATG 2 cut(s) 135, 999
BsrI ACTGG 3 cut(s) 113, 332, 1729
BssECI CCNNGG 5 cut(s) 102, 160, 482, 1398, 1431
BssMI GATC 3 cut(s) 64, 478, 1342
BssT1I CCWWGG 5 cut(s) 102, 160, 482, 1398, 1431
Bst2UI CCWGG 1 cut(s) 468
Bst4CI ACNGT 4 cut(s) 352, 975, 1223, 1382
Bst6I CTCTTC 1 cut(s) 1503
BstAFI CTTAAG 1 cut(s) 725
BstAPI GCANNNNNTGC 2 cut(s) 47, 130
BstBAI YACGTR 1 cut(s) 1710
BstBI TTCGAA 1 cut(s) 1412
BstC8I GCNNGC 6 cut(s) 126, 822, 877, 1247, 1494, 1527
BstDEI CTNAG 6 cut(s) 378, 579, 642, 825, 1079, 1698
BstDSI CCRYGG 1 cut(s) 102
BstEII GGTNACC 1 cut(s) 1169
BstF5I GGATG 3 cut(s) 971, 1459, 1625
BstHHI GCGC 1 cut(s) 1074
BstKTI GATC 3 cut(s) 67, 481, 1345
BstMAI GTCTC 3 cut(s) 314, 349, 1264
BstMBI GATC 3 cut(s) 64, 478, 1342
BstMWI GCNNNNNNNGC 8 cut(s) 47, 56, 130, 374, 545, 830, 1304, 1700
BstNI CCWGG 1 cut(s) 468
BstNSI RCATGY 1 cut(s) 1445
BstPI GGTNACC 1 cut(s) 1169
BstSCI CCNGG 1 cut(s) 466
BstSFI CTRYAG 2 cut(s) 54, 75
BstV1I GCAGC 4 cut(s) 68, 386, 1111, 1311
BstV2I GAAGAC 1 cut(s) 1260
BstX2I RGATCY 1 cut(s) 478
BstXI CCANNNNNNTGG 3 cut(s) 467, 549, 1233
BstYI RGATCY 1 cut(s) 478
BsuRI GGCC 4 cut(s) 101, 1280, 1403, 1481
BtgI CCRYGG 1 cut(s) 102
BtsCI GGATG 3 cut(s) 971, 1459, 1625
BtsI GCAGTG 1 cut(s) 1302
BtsIMutI CAGTG 2 cut(s) 1302, 1378
Cac8I GCNNGC 6 cut(s) 126, 822, 877, 1247, 1494, 1527
CfoI GCGC 1 cut(s) 1074
Cfr13I GGNCC 2 cut(s) 635, 1402
CsiI ACCWGGT 1 cut(s) 466
Csp6I GTAC 6 cut(s) 165, 949, 1052, 1084, 1095, 1285
CspCI CAANNNNNGTGG 2 cut(s) 1671, 1706
CviAII CATG 6 cut(s) 103, 428, 497, 888, 1349, 1442
CviQI GTAC 6 cut(s) 165, 949, 1052, 1084, 1095, 1285
DdeI CTNAG 6 cut(s) 378, 579, 642, 825, 1079, 1698
DpnI GATC 3 cut(s) 66, 480, 1344
DpnII GATC 3 cut(s) 64, 478, 1342
DraI TTTAAA 1 cut(s) 934
DriI GACNNNNNGTC 1 cut(s) 327
EaeI YGGCCR 1 cut(s) 1479
Eam1104I CTCTTC 1 cut(s) 1503
Eam1105I GACNNNNNGTC 1 cut(s) 327
EarI CTCTTC 1 cut(s) 1503
Ecl136II GAGCTC 2 cut(s) 926, 1239
Eco130I CCWWGG 5 cut(s) 102, 160, 482, 1398, 1431
Eco24I GRGCYC 2 cut(s) 928, 1241
Eco32I GATATC 1 cut(s) 1534
Eco47I GGWCC 1 cut(s) 635
Eco53kI GAGCTC 2 cut(s) 926, 1239
Eco57I CTGAAG 3 cut(s) 1239, 1293, 1484
Eco72I CACGTG 1 cut(s) 1710
Eco91I GGTNACC 1 cut(s) 1169
EcoICRI GAGCTC 2 cut(s) 926, 1239
EcoO65I GGTNACC 1 cut(s) 1169
EcoRII CCWGG 1 cut(s) 466
EcoRV GATATC 1 cut(s) 1534
EcoT14I CCWWGG 5 cut(s) 102, 160, 482, 1398, 1431
EcoT22I ATGCAT 1 cut(s) 151
EcoT38I GRGCYC 2 cut(s) 928, 1241
ErhI CCWWGG 5 cut(s) 102, 160, 482, 1398, 1431
FaeI CATG 6 cut(s) 106, 431, 500, 891, 1352, 1445
FalI AAGNNNNNCTT 2 cut(s) 708, 740
FaqI GGGAC 3 cut(s) 442, 621, 768
FatI CATG 6 cut(s) 102, 427, 496, 887, 1348, 1441
Fnu4HI GCNGC 4 cut(s) 57, 375, 1125, 1325
FokI GGATG 3 cut(s) 978, 1466, 1632
FriOI GRGCYC 2 cut(s) 928, 1241
Fsp4HI GCNGC 4 cut(s) 57, 375, 1125, 1325
FspBI CTAG 6 cut(s) 9, 404, 797, 1467, 1493, 1526
GlaI GCGC 1 cut(s) 1073
GluI GCNGC 4 cut(s) 57, 375, 1125, 1325
HaeIII GGCC 4 cut(s) 101, 1280, 1403, 1481
HhaI GCGC 1 cut(s) 1074
Hin1II CATG 6 cut(s) 106, 431, 500, 891, 1352, 1445
Hin6I GCGC 1 cut(s) 1072
HinP1I GCGC 1 cut(s) 1072
HinfI GANTC 4 cut(s) 561, 1154, 1414, 1553
HphI GGTGA 4 cut(s) 556, 979, 1181, 1499
Hpy166II GTNNAC 1 cut(s) 1658
Hpy188I TCNGA 4 cut(s) 244, 1101, 1159, 1312
Hpy188III TCNNGA 5 cut(s) 296, 845, 1537, 1643, 1733
Hpy8I GTNNAC 1 cut(s) 1658
HpyAV CCTTC 9 cut(s) 31, 93, 296, 433, 724, 739, 1179, 1371, 1521
HpyCH4III ACNGT 4 cut(s) 352, 975, 1223, 1382
HpyCH4IV ACGT 2 cut(s) 947, 1709
HpyF10VI GCNNNNNNNGC 8 cut(s) 47, 56, 130, 374, 545, 830, 1304, 1700
HpyF3I CTNAG 6 cut(s) 378, 579, 642, 825, 1079, 1698
HpySE526I ACGT 2 cut(s) 947, 1709
Hsp92II CATG 6 cut(s) 106, 431, 500, 891, 1352, 1445
HspAI GCGC 1 cut(s) 1072
Kzo9I GATC 3 cut(s) 64, 478, 1342
LguI GCTCTTC 1 cut(s) 1503
LmnI GCTCC 8 cut(s) 254, 544, 923, 931, 1188, 1236, 1372, 1583
Lsp1109I GCAGC 4 cut(s) 68, 386, 1111, 1311
LweI GCATC 6 cut(s) 136, 709, 888, 956, 1578, 1612
MabI ACCWGGT 1 cut(s) 466
MaeI CTAG 6 cut(s) 9, 404, 797, 1467, 1493, 1526
MaeII ACGT 2 cut(s) 947, 1709
MaeIII GTNAC 7 cut(s) 507, 516, 1169, 1217, 1356, 1382, 1741
MalI GATC 3 cut(s) 66, 480, 1344
MboI GATC 3 cut(s) 64, 478, 1342
MboII GAAGA 8 cut(s) 78, 149, 311, 416, 455, 1260, 1337, 1490
MflI RGATCY 1 cut(s) 478
MhlI GDGCHC 3 cut(s) 928, 1193, 1241
MlsI TGGCCA 1 cut(s) 1481
MluNI TGGCCA 1 cut(s) 1481
MlyI GAGTC 1 cut(s) 1148
MmeI TCCRAC 3 cut(s) 515, 1067, 1605
Mox20I TGGCCA 1 cut(s) 1481
Mph1103I ATGCAT 1 cut(s) 151
MscI TGGCCA 1 cut(s) 1481
MseI TTAA 9 cut(s) 152, 225, 411, 531, 624, 714, 726, 933, 1139
Msp20I TGGCCA 1 cut(s) 1481
MspCI CTTAAG 1 cut(s) 725
MspR9I CCNGG 1 cut(s) 468
Mva1269I GAATGC 1 cut(s) 1074
MvaI CCWGG 1 cut(s) 468
MwoI GCNNNNNNNGC 8 cut(s) 47, 56, 130, 374, 545, 830, 1304, 1700
NcoI CCATGG 1 cut(s) 102
NdeII GATC 3 cut(s) 64, 478, 1342
NheI GCTAGC 2 cut(s) 1492, 1525
NlaIII CATG 6 cut(s) 106, 431, 500, 891, 1352, 1445
NlaIV GGNNCC 4 cut(s) 250, 480, 637, 1597
NmeAIII GCCGAG 1 cut(s) 1306
NmuCI GTSAC 3 cut(s) 516, 1169, 1382
NsiI ATGCAT 1 cut(s) 151
NspI RCATGY 1 cut(s) 1445
NspV TTCGAA 1 cut(s) 1412
PciSI GCTCTTC 1 cut(s) 1503
PctI GAATGC 1 cut(s) 1074
PfeI GAWTC 3 cut(s) 561, 1414, 1553
PkrI GCNGC 4 cut(s) 58, 376, 1126, 1326
PleI GAGTC 1 cut(s) 1148
PmaCI CACGTG 1 cut(s) 1710
PmlI CACGTG 1 cut(s) 1710
PpsI GAGTC 1 cut(s) 1148
Ppu21I YACGTR 1 cut(s) 1710
Psp124BI GAGCTC 2 cut(s) 928, 1241
Psp6I CCWGG 1 cut(s) 466
PspCI CACGTG 1 cut(s) 1710
PspEI GGTNACC 1 cut(s) 1169
PspGI CCWGG 1 cut(s) 466
PspN4I GGNNCC 4 cut(s) 250, 480, 637, 1597
PspPI GGNCC 2 cut(s) 635, 1402
PstI CTGCAG 1 cut(s) 58
PsuI RGATCY 1 cut(s) 478
RsaI GTAC 6 cut(s) 166, 950, 1053, 1085, 1096, 1286
RsaNI GTAC 6 cut(s) 165, 949, 1052, 1084, 1095, 1285
SacI GAGCTC 2 cut(s) 928, 1241
SapI GCTCTTC 1 cut(s) 1503
SaqAI TTAA 9 cut(s) 152, 225, 411, 531, 624, 714, 726, 933, 1139
SatI GCNGC 4 cut(s) 57, 375, 1125, 1325
Sau3AI GATC 3 cut(s) 64, 478, 1342
Sau96I GGNCC 2 cut(s) 635, 1402
SchI GAGTC 1 cut(s) 1148
ScrFI CCNGG 1 cut(s) 468
SduI GDGCHC 3 cut(s) 928, 1193, 1241
SexAI ACCWGGT 1 cut(s) 466
SfaNI GCATC 6 cut(s) 136, 709, 888, 956, 1578, 1612
SfcI CTRYAG 2 cut(s) 54, 75
SfuI TTCGAA 1 cut(s) 1412
SinI GGWCC 1 cut(s) 635
SmlI CTYRAG 3 cut(s) 186, 725, 1261
SmoI CTYRAG 3 cut(s) 186, 725, 1261
SspI AATATT 1 cut(s) 27
SspMI CTAG 6 cut(s) 9, 404, 797, 1467, 1493, 1526
SstI GAGCTC 2 cut(s) 928, 1241
StyD4I CCNGG 1 cut(s) 466
StyI CCWWGG 5 cut(s) 102, 160, 482, 1398, 1431
TaaI ACNGT 4 cut(s) 352, 975, 1223, 1382
TaiI ACGT 2 cut(s) 950, 1712
TaqI TCGA 1 cut(s) 1412
TatI WGTACW 2 cut(s) 1051, 1284
TfiI GAWTC 3 cut(s) 561, 1414, 1553
Tru1I TTAA 9 cut(s) 152, 225, 411, 531, 624, 714, 726, 933, 1139
Tru9I TTAA 9 cut(s) 152, 225, 411, 531, 624, 714, 726, 933, 1139
TscAI CASTG 2 cut(s) 1309, 1385
TseFI GTSAC 3 cut(s) 516, 1169, 1382
TseI GCWGC 4 cut(s) 56, 374, 1124, 1324
Tsp45I GTSAC 3 cut(s) 516, 1169, 1382
TspDTI ATGAA 8 cut(s) 78, 416, 570, 610, 758, 802, 1337, 1412
TspRI CASTG 2 cut(s) 1309, 1385
Vha464I CTTAAG 1 cut(s) 725
VpaK11BI GGWCC 1 cut(s) 635
XapI RAATTY 3 cut(s) 140, 238, 734
XceI RCATGY 1 cut(s) 1445
XspI CTAG 6 cut(s) 9, 404, 797, 1467, 1493, 1526
Zsp2I ATGCAT 1 cut(s) 151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.