Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0001471

Physical Location & Seq

Genomic Coordinates Reverse (-)

12582084 .. 12586685

Gene Structure 4602 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G011150.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2337 bp

ATGGCTCTCATGAGTTTGCTTATGGCTACTTTAGATGAGGATATTGACAAGGTATCAAAGATAATTCTCTATCAAGGAAAGAGCAGTGAAGATGGCTTGTTTCTCTTCAAGACACCACAACTTTCAGCAATAAACCAACTATCTCATACTGCTTTGGTTGGATCAGCTCCTACTTCTTCTAAGGAAAAATGCATAGATTGTCATTTCCTGAATCTGCATCACATTGTACTGTACCTTTCCACAAAATACATTCTGATGATGCCCTGCAAGTCTCTTCACATGTCTTCTCCATATGAACTTCTATTTCACAAGCCTCCTGAGGTGTCTCAGCTTAAGGTGTTTGGTTCTTCTTGCTATCCATTTCTTAGACCCTATACTCATGATAAGCTTGATCCAAGAACAACTCAGTGTGTGTTCTTAGGTTATGCTCTCGGATATAAAGGAGTCTTTTGCTATAGTATTTCTCAAAACAAACTGTGGATGTCAAGACATGTTGTTCATGATGAAACCATATTCCCATATCTGTTACCTTCTCAACCTTCAACCTCACGGCCTGATCCTGTGACTTACTTTACTAGTTCTTCTTGGTCACCTGCATCTGTGCAGTCTTCTTCTATATCCTGTGCTCCGACTCCTGTTGCTCCTTCCACTGAGGTCCAAGGTGAATCTATTCATGAAGTGTTGTTGAATGGTGATCATAGCACTGATAATCTTCATGCCAATCATCAATCACCAGACCACCCTGCAAGTGACTCACAACCTCAAGTAGTTGATTCAATGGGTGGTACCATTGAAACTGTTCTTCCTCTGAATAGTGTTTCTGATGATATGCAAGCTATGCAAGTTGGCCATGAACACACATCTTCTGCTTGTGTTCCTGATGCTATGCAAGTTGATCAAGAACACACATCTTCTGTTGCACCTGATCATCCTAGTATTGCACCTTTTCAGGGCCTCCATACAAATGAGCTCAATACCTTACAATTTGGAGGACTGAATAATACTGCTGCAGATGCTTTACCTCAAGACCTCACCTTCATCATGGATCAAAACTACAATGATCACTCTATGATGACTCGGGCCAAGAATGGCATCATCAAATCTAAAACATTTGAAGACTATTTTTGCTTCTCTGCCATTGTTCAACAGAAACTCTATGATGAATATAAGGCCAGGTTGGTTGCACAAGGCTACAGTCAATCAAAAGGCCTGGATTATGATGAGACCTTCAGTCCTGTTGTGAGGCACAGCACTGTCAGAGTGATCATTGCACTGGCTGCAATGAATAATTGGGAACTGCGACAGTTAGATGTGAAAAATGCATTTCTGCATGGAGATTTGAAAGAAGAAGTTTATATGGCTCAACCTCCTGGTTTTGTTGATCCAGTACACAGGGATTATGTATGTCTTTTAAAGAAATCTCTGTATGGATTAAAGCAAGCTCCACGTGCATGGAATGACAAATTCACAAGCTTTCTTCCAAATGATATTGTGATTACTGGTTCTGATACCACTGGAATCCATGAGGTGATATCGGAGCTCAGTATGGTCTTTGATATGAAAGATCTTGGTCCTTTGACTTATTTTCTTGGTCTGGAAGTTACCAAGGTGAAACAAGGAATATTTCTGGGTCAAACTAAGTATGCTAAAGATCTCCTTGTCAAGGCAGGAATGGATACAGTTCGTCCGTGTAGTTCTCCTTGTCTCCCTCATTGTCATATGACTAAAGATCAAGTCCCTACTGATGTTCATTTTAGTGCTGTCAAGCGAATACTTAGATATCTTCAGGGCACTACTCATAAAGGCTTGTTTTATAGCTCAGCTCATGCTTTGTCTAATGCAGTACATCTCCAGGCATTCTGTGATGCAGACTGGGCAGGTGAAGTTATTCAACGTCGATCAACCACTGGATTTGTAGTTTCTCTTGGTCTATGTCCTATTTCATGGCAGTCAAAGAAACAAGGCACCGTTTCCCGAAGTTCCACTGAAGCTGAATATAGAAGCCTGGCTAATACTGCAGCTGAAATAAGTTGGATTAGGCATCTTCTTTGTGACCTGAGGATTTCTATTCCTACGGCTCCCTTGATTAAGTGTGATAACTTGTCTGCAATAGCTCTTGCCTCCAATCCAGTGTTTCACTCTAAGATCAAACACTTGGATAATGATTTCCATTTTGTTCGCGAACGTGTTCAGCGCAACGATCTGTGTCTTGCTTATGTACCTACAACTGATCAAACTGCAGACATATTGACCAAAGGGTTACCTTCTCCTCTCTTTCGCACTCATTGTATCAATCTCAGGCTAGTTACCCTAGCTGAGCTTGAGGAGGGATGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RVT_2 Retrotran_gag_2 Retrotran_gag_3 Retrotrans_gag gag_pre-integrs rve

Protein Analysis

778

Amino Acids

86.57

Weight (kDa)

6.19

Isoelectric Point (pI)

44.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SH3_retrovirus	PF25597	116 - 176	6.4e-16	Retroviral polymerase SH3-like domain
RVT_2	PF07727	385 - 493	1.4e-39	Reverse transcriptase (RNA-dependent DNA polymerase)
RVT_2	PF07727	495 - 569	1e-10	Reverse transcriptase (RNA-dependent DNA polymerase)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001471)

Species	Orthologous Gene IDs
arabidopsis_thaliana	ATMG00240
fragaria_vesca	FvH4_1g25543 FvH4_3g07841 FvH4_3g14871 FvH4_4g13042 FvH4_4g19532 FvH4_4g27904 FvH4_5g11311 FvH4_5g24711 FvH4_6g16681
malus_domestica	MD01G1044800.v1.1 MD05G1032500.v1.1 MD14G1067200.v1.1
pyrus_communis	pycom02g08540 pycom02g19080 pycom03g19430 pycom05g02910 pycom15g04590 pycom16g02160 pycom16g25210
rosa_chinensis	RchiOBHm_Chr1g0313691 RchiOBHm_Chr1g0339121 RchiOBHm_Chr2g0119341 RchiOBHm_Chr2g0141981 RchiOBHm_Chr2g0155191 RchiOBHm_Chr2g0175291 RchiOBHm_Chr3g0475621 RchiOBHm_Chr3g0482011 RchiOBHm_Chr4g0401001 RchiOBHm_Chr5g0038401 RchiOBHm_Chr6g0253961 RchiOBHm_Chr6g0266711 RchiOBHm_Chr7g0198221 RchiOBHm_Chr7g0200081
rosa_laevigata	RLG00000035859
rosa_roxburghii	Rroxscaffold_4G00281750
rosa_wichuraiana	Rw1G008180 Rw1G040520 Rw2G014210 Rw2G020850 Rw2G043190 Rw2G049320 Rw4G005820 Rw4G021520 Rw5G011150 Rw5G016420 Rw6G010970 Rw7G015120 Rw7G015940 Rw7G019550 Rw7G023080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	601, 1868
Acc36I	ACCTGC	2 cut(s)	601, 1868
Acc65I	GGTACC	1 cut(s)	785
AccB1I	GGYRCC	2 cut(s)	785, 1964
AccII	CGCG	1 cut(s)	2181
AclWI	GGATC	5 cut(s)	169, 386, 551, 1053, 1376
AcoI	YGGCCR	1 cut(s)	847
AcsI	RAATTY	1 cut(s)	1463
AcuI	CTGAAG	3 cut(s)	1213, 1769, 2007
AcvI	CACGTG	1 cut(s)	1448
AfaI	GTAC	6 cut(s)	228, 233, 787, 1389, 1845, 2220
AfiI	CCNNNNNNNGG	2 cut(s)	950, 1663
AflII	CTTAAG	1 cut(s)	332
AflIII	ACRYGT	3 cut(s)	279, 490, 2185
AgsI	TTSAA	9 cut(s)	109, 543, 688, 777, 794, 1115, 1145, 1342, 1892
AhdI	GACNNNNNGTC	1 cut(s)	1230
AhlI	ACTAGT	1 cut(s)	575
AjnI	CCWGG	5 cut(s)	1172, 1209, 1369, 1851, 2004
Alw21I	GWGCWC	3 cut(s)	628, 972, 1542
Alw26I	GTCTC	4 cut(s)	276, 330, 1217, 1709
AlwI	GGATC	5 cut(s)	169, 386, 551, 1053, 1376
Ama87I	CYCGRG	1 cut(s)	1077
AoxI	GGCC	6 cut(s)	551, 847, 952, 1080, 1170, 1207
ApeKI	GCWGC	3 cut(s)	1007, 1277, 2018
ApoI	RAATTY	1 cut(s)	1463
Asp700I	GAANNNNTTC	4 cut(s)	669, 798, 1887, 2187
Asp718I	GGTACC	1 cut(s)	785
AspLEI	GCGC	1 cut(s)	2196
AspS9I	GGNCC	4 cut(s)	655, 952, 1080, 1571
AsuHPI	GGTGA	8 cut(s)	582, 674, 704, 723, 1024, 1540, 1621, 1892
AvaI	CYCGRG	1 cut(s)	1077
AvaII	GGWCC	2 cut(s)	655, 1571
AxyI	CCTNAGG	2 cut(s)	318, 2057
BaeGI	GKGCMC	1 cut(s)	1793
BalI	TGGCCA	1 cut(s)	849
BanI	GGYRCC	2 cut(s)	785, 1964
BanII	GRGCYC	2 cut(s)	972, 1542
BbrPI	CACGTG	1 cut(s)	1448
BbsI	GAAGAC	3 cut(s)	276, 600, 1122
Bbv12I	GWGCWC	3 cut(s)	628, 972, 1542
BbvI	GCAGC	3 cut(s)	994, 1264, 2030
BccI	CCATC	1 cut(s)	86
BceAI	ACGGC	2 cut(s)	566, 2091
BciT130I	CCWGG	5 cut(s)	1174, 1211, 1371, 1853, 2006
BciVI	GTATCC	1 cut(s)	1669
BclI	TGATCA	6 cut(s)	694, 895, 925, 1060, 1263, 2230
BcoDI	GTCTC	4 cut(s)	276, 330, 1217, 1709
BcuI	ACTAGT	1 cut(s)	575
BfaI	CTAG	4 cut(s)	576, 933, 2303, 2312
BfmI	CTRYAG	5 cut(s)	454, 1008, 1192, 2016, 2238
BfrI	CTTAAG	1 cut(s)	332
BfuAI	ACCTGC	2 cut(s)	601, 1868
BfuI	GTATCC	1 cut(s)	1669
BglII	AGATCT	2 cut(s)	1564, 1651
BisI	GCNGC	3 cut(s)	1008, 1278, 2019
BlpI	GCTNAGC	2 cut(s)	1819, 2316
BlsI	GCNGC	3 cut(s)	1009, 1279, 2020
Bme1390I	CCNGG	5 cut(s)	1174, 1211, 1371, 1853, 2006
Bme18I	GGWCC	2 cut(s)	655, 1571
BmeRI	GACNNNNNGTC	1 cut(s)	1230
BmeT110I	CYCGRG	1 cut(s)	1077
BmgT120I	GGNCC	4 cut(s)	655, 952, 1080, 1571
BmiI	GGNNCC	3 cut(s)	787, 1966, 2079
BmrFI	CCNGG	5 cut(s)	1174, 1211, 1371, 1853, 2006
BmrI	ACTGGG	1 cut(s)	1882
BmsI	GCATC	8 cut(s)	226, 249, 605, 871, 1003, 1101, 1855, 2050
BmuI	ACTGGG	1 cut(s)	1882
BpiI	GAAGAC	3 cut(s)	276, 600, 1122
BpmI	CTGGAG	1 cut(s)	1835
Bpu1102I	GCTNAGC	2 cut(s)	1819, 2316
BpuEI	CTTGAG	2 cut(s)	747, 1008
BsaAI	YACGTR	1 cut(s)	1448
BsaI	GGTCTC	1 cut(s)	1217
BsaJI	CCNNGG	2 cut(s)	658, 1605
Bsc4I	CCNNNNNNNGG	2 cut(s)	950, 1663
Bse1I	ACTGG	7 cut(s)	1278, 1385, 1504, 1519, 1877, 1912, 2129
Bse21I	CCTNAGG	2 cut(s)	318, 2057
Bse3DI	GCAATG	2 cut(s)	1266, 1287
BseBI	CCWGG	5 cut(s)	1174, 1211, 1371, 1853, 2006
BseDI	CCNNGG	2 cut(s)	658, 1605
BseGI	GGATG	3 cut(s)	486, 928, 2336
BseLI	CCNNNNNNNGG	2 cut(s)	950, 1663
BseMI	GCAATG	2 cut(s)	1266, 1287
BseMII	CTCAG	9 cut(s)	309, 341, 419, 642, 1555, 1833, 2048, 2307, 2311
BseNI	ACTGG	7 cut(s)	1278, 1385, 1504, 1519, 1877, 1912, 2129
BseRI	GAGGAG	1 cut(s)	2259
BseSI	GKGCMC	1 cut(s)	1793
BseXI	GCAGC	3 cut(s)	994, 1264, 2030
BsgI	GTGCAG	1 cut(s)	623
Bsh1236I	CGCG	1 cut(s)	2181
BshFI	GGCC	6 cut(s)	553, 849, 954, 1082, 1172, 1209
BshNI	GGYRCC	2 cut(s)	785, 1964
BsiHKAI	GWGCWC	3 cut(s)	628, 972, 1542
BsiHKCI	CYCGRG	1 cut(s)	1077
BslFI	GGGAC	1 cut(s)	1721
BslI	CCNNNNNNNGG	2 cut(s)	950, 1663
BsmAI	GTCTC	4 cut(s)	276, 330, 1217, 1709
BsmFI	GGGAC	1 cut(s)	1721
BsmI	GAATGC	1 cut(s)	1856
BsnI	GGCC	6 cut(s)	553, 849, 954, 1082, 1172, 1209
Bso31I	GGTCTC	1 cut(s)	1217
BsoBI	CYCGRG	1 cut(s)	1077
Bsp1286I	GDGCHC	4 cut(s)	628, 972, 1542, 1793
Bsp1720I	GCTNAGC	2 cut(s)	1819, 2316
Bsp68I	TCGCGA	1 cut(s)	2181
BspANI	GGCC	6 cut(s)	553, 849, 954, 1082, 1172, 1209
BspCNI	CTCAG	9 cut(s)	310, 340, 418, 643, 1554, 1832, 2049, 2308, 2310
BspFNI	CGCG	1 cut(s)	2181
BspHI	TCATGA	4 cut(s)	9, 379, 499, 673
BspLI	GGNNCC	3 cut(s)	787, 1966, 2079
BspMAI	CTGCAG	3 cut(s)	1012, 2020, 2242
BspMI	ACCTGC	2 cut(s)	601, 1868
BspPI	GGATC	5 cut(s)	169, 386, 551, 1053, 1376
BspT107I	GGYRCC	2 cut(s)	785, 1964
BspTI	CTTAAG	1 cut(s)	332
BspTNI	GGTCTC	1 cut(s)	1217
BsrDI	GCAATG	2 cut(s)	1266, 1287
BsrI	ACTGG	7 cut(s)	1278, 1385, 1504, 1519, 1877, 1912, 2129
BssECI	CCNNGG	2 cut(s)	658, 1605
BssT1I	CCWWGG	2 cut(s)	658, 1605
Bst2UI	CCWGG	5 cut(s)	1174, 1211, 1371, 1853, 2006
Bst4CI	ACNGT	8 cut(s)	231, 477, 799, 1196, 1255, 1305, 1681, 1969
Bst6I	CTCTTC	2 cut(s)	110, 279
BstAFI	CTTAAG	1 cut(s)	332
BstAPI	GCANNNNNTGC	2 cut(s)	838, 1277
BstBAI	YACGTR	1 cut(s)	1448
BstC8I	GCNNGC	2 cut(s)	834, 1440
BstEII	GGTNACC	2 cut(s)	588, 2259
BstENI	CCTNNNNNAGG	1 cut(s)	1661
BstF5I	GGATG	3 cut(s)	486, 928, 2336
BstFNI	CGCG	1 cut(s)	2181
BstHHI	GCGC	1 cut(s)	2196
BstMAI	GTCTC	4 cut(s)	276, 330, 1217, 1709
BstMWI	GCNNNNNNNGC	6 cut(s)	838, 1013, 1277, 1448, 1874, 2015
BstNI	CCWGG	5 cut(s)	1174, 1211, 1371, 1853, 2006
BstNSI	RCATGY	2 cut(s)	283, 494
BstPI	GGTNACC	2 cut(s)	588, 2259
BstSCI	CCNGG	5 cut(s)	1172, 1209, 1369, 1851, 2004
BstSFI	CTRYAG	5 cut(s)	454, 1008, 1192, 2016, 2238
BstSLI	GKGCMC	1 cut(s)	1793
BstUI	CGCG	1 cut(s)	2181
BstV1I	GCAGC	3 cut(s)	994, 1264, 2030
BstV2I	GAAGAC	3 cut(s)	276, 600, 1122
BstX2I	RGATCY	2 cut(s)	1564, 1651
BstXI	CCANNNNNNTGG	1 cut(s)	1452
BstYI	RGATCY	2 cut(s)	1564, 1651
Bsu36I	CCTNAGG	2 cut(s)	318, 2057
BsuI	GTATCC	1 cut(s)	1669
BsuRI	GGCC	6 cut(s)	553, 849, 954, 1082, 1172, 1209
BtsCI	GGATG	3 cut(s)	486, 928, 2336
BtsI	GCAGTG	1 cut(s)	91
BtuMI	TCGCGA	1 cut(s)	2181
BveI	ACCTGC	2 cut(s)	601, 1868
Cac8I	GCNNGC	2 cut(s)	834, 1440
CciI	TCATGA	4 cut(s)	9, 379, 499, 673
CfoI	GCGC	1 cut(s)	2196
Cfr13I	GGNCC	4 cut(s)	655, 952, 1080, 1571
Csp6I	GTAC	6 cut(s)	227, 232, 786, 1388, 1844, 2219
CspCI	CAANNNNNGTGG	2 cut(s)	1894, 1929
CviQI	GTAC	6 cut(s)	227, 232, 786, 1388, 1844, 2219
DraI	TTTAAA	1 cut(s)	1413
DriI	GACNNNNNGTC	1 cut(s)	1230
EaeI	YGGCCR	1 cut(s)	847
Eam1104I	CTCTTC	2 cut(s)	110, 279
Eam1105I	GACNNNNNGTC	1 cut(s)	1230
EarI	CTCTTC	2 cut(s)	110, 279
Ecl136II	GAGCTC	2 cut(s)	970, 1540
Eco130I	CCWWGG	2 cut(s)	658, 1605
Eco147I	AGGCCT	1 cut(s)	1209
Eco24I	GRGCYC	2 cut(s)	972, 1542
Eco31I	GGTCTC	1 cut(s)	1217
Eco32I	GATATC	2 cut(s)	1533, 1781
Eco47I	GGWCC	2 cut(s)	655, 1571
Eco53kI	GAGCTC	2 cut(s)	970, 1540
Eco57I	CTGAAG	3 cut(s)	1213, 1769, 2007
Eco72I	CACGTG	1 cut(s)	1448
Eco81I	CCTNAGG	2 cut(s)	318, 2057
Eco88I	CYCGRG	1 cut(s)	1077
Eco91I	GGTNACC	2 cut(s)	588, 2259
EcoICRI	GAGCTC	2 cut(s)	970, 1540
EcoNI	CCTNNNNNAGG	1 cut(s)	1661
EcoO109I	RGGNCCY	1 cut(s)	952
EcoO65I	GGTNACC	2 cut(s)	588, 2259
EcoRII	CCWGG	5 cut(s)	1172, 1209, 1369, 1851, 2004
EcoRV	GATATC	2 cut(s)	1533, 1781
EcoT14I	CCWWGG	2 cut(s)	658, 1605
EcoT22I	ATGCAT	2 cut(s)	194, 1324
EcoT38I	GRGCYC	2 cut(s)	972, 1542
ErhI	CCWWGG	2 cut(s)	658, 1605
FalI	AAGNNNNNCTT	2 cut(s)	1641, 1673
FaqI	GGGAC	1 cut(s)	1721
FauNDI	CATATG	2 cut(s)	292, 1719
FbaI	TGATCA	6 cut(s)	694, 895, 925, 1060, 1263, 2230
Fnu4HI	GCNGC	3 cut(s)	1008, 1278, 2019
FokI	GGATG	2 cut(s)	493, 915
FriOI	GRGCYC	2 cut(s)	972, 1542
Fsp4HI	GCNGC	3 cut(s)	1008, 1278, 2019
FspBI	CTAG	4 cut(s)	576, 933, 2303, 2312
GlaI	GCGC	1 cut(s)	2195
GluI	GCNGC	3 cut(s)	1008, 1278, 2019
GsuI	CTGGAG	1 cut(s)	1835
HaeIII	GGCC	6 cut(s)	553, 849, 954, 1082, 1172, 1209
HhaI	GCGC	1 cut(s)	2196
Hin6I	GCGC	1 cut(s)	2194
HinP1I	GCGC	1 cut(s)	2194
HindIII	AAGCTT	2 cut(s)	386, 1471
HinfI	GANTC	8 cut(s)	211, 444, 631, 665, 752, 773, 1075, 1518
HphI	GGTGA	8 cut(s)	582, 674, 704, 723, 1024, 1540, 1621, 1892
Hpy166II	GTNNAC	1 cut(s)	1390
Hpy188I	TCNGA	8 cut(s)	255, 434, 630, 810, 823, 1259, 1507, 1537
Hpy8I	GTNNAC	1 cut(s)	1390
Hpy99I	CGWCG	1 cut(s)	1899
HpyAV	CCTTC	6 cut(s)	540, 549, 654, 1045, 1237, 2274
HpyCH4III	ACNGT	8 cut(s)	231, 477, 799, 1196, 1255, 1305, 1681, 1969
HpyCH4IV	ACGT	3 cut(s)	1447, 1894, 2185
HpyF10VI	GCNNNNNNNGC	6 cut(s)	838, 1013, 1277, 1448, 1874, 2015
HpySE526I	ACGT	3 cut(s)	1447, 1894, 2185
HspAI	GCGC	1 cut(s)	2194
KpnI	GGTACC	1 cut(s)	789
Ksp22I	TGATCA	6 cut(s)	694, 895, 925, 1060, 1263, 2230
LmnI	GCTCC	6 cut(s)	172, 631, 646, 1447, 1537, 2083
Lsp1109I	GCAGC	3 cut(s)	994, 1264, 2030
LweI	GCATC	8 cut(s)	226, 249, 605, 871, 1003, 1101, 1855, 2050
MaeI	CTAG	4 cut(s)	576, 933, 2303, 2312
MaeII	ACGT	3 cut(s)	1447, 1894, 2185
MaeIII	GTNAC	8 cut(s)	525, 562, 588, 749, 1600, 2051, 2259, 2305
MflI	RGATCY	2 cut(s)	1564, 1651
MhlI	GDGCHC	4 cut(s)	628, 972, 1542, 1793
MlsI	TGGCCA	1 cut(s)	849
MluCI	AATT	4 cut(s)	63, 983, 1288, 1463
MluNI	TGGCCA	1 cut(s)	849
MlyI	GAGTC	4 cut(s)	453, 625, 746, 1069
MmeI	TCCRAC	3 cut(s)	139, 653, 2012
Mox20I	TGGCCA	1 cut(s)	849
Mph1103I	ATGCAT	2 cut(s)	194, 1324
MroXI	GAANNNNTTC	4 cut(s)	669, 798, 1887, 2187
MscI	TGGCCA	1 cut(s)	849
MseI	TTAA	5 cut(s)	333, 1412, 1433, 2088, 2335
MslI	CAYNNNNRTG	4 cut(s)	254, 963, 1450, 1755
Msp20I	TGGCCA	1 cut(s)	849
MspA1I	CMGCKG	1 cut(s)	2021
MspCI	CTTAAG	1 cut(s)	332
MspR9I	CCNGG	5 cut(s)	1174, 1211, 1371, 1853, 2006
Mva1269I	GAATGC	1 cut(s)	1856
MvaI	CCWGG	5 cut(s)	1174, 1211, 1371, 1853, 2006
MvnI	CGCG	1 cut(s)	2181
MwoI	GCNNNNNNNGC	6 cut(s)	838, 1013, 1277, 1448, 1874, 2015
NdeI	CATATG	2 cut(s)	292, 1719
NlaIV	GGNNCC	3 cut(s)	787, 1966, 2079
NmuCI	GTSAC	4 cut(s)	562, 588, 749, 2051
NruI	TCGCGA	1 cut(s)	2181
NsiI	ATGCAT	2 cut(s)	194, 1324
NspI	RCATGY	2 cut(s)	283, 494
PagI	TCATGA	4 cut(s)	9, 379, 499, 673
PaqCI	CACCTGC	2 cut(s)	601, 1868
PceI	AGGCCT	1 cut(s)	1209
PciI	ACATGT	2 cut(s)	279, 490
PctI	GAATGC	1 cut(s)	1856
PdmI	GAANNNNTTC	4 cut(s)	669, 798, 1887, 2187
PfeI	GAWTC	4 cut(s)	211, 665, 773, 1518
PkrI	GCNGC	3 cut(s)	1009, 1279, 2020
PleI	GAGTC	4 cut(s)	452, 625, 746, 1069
PmaCI	CACGTG	1 cut(s)	1448
PmlI	CACGTG	1 cut(s)	1448
PpsI	GAGTC	4 cut(s)	452, 625, 746, 1069
Ppu21I	YACGTR	1 cut(s)	1448
PscI	ACATGT	2 cut(s)	279, 490
Psp124BI	GAGCTC	2 cut(s)	972, 1542
Psp6I	CCWGG	5 cut(s)	1172, 1209, 1369, 1851, 2004
PspCI	CACGTG	1 cut(s)	1448
PspEI	GGTNACC	2 cut(s)	588, 2259
PspGI	CCWGG	5 cut(s)	1172, 1209, 1369, 1851, 2004
PspN4I	GGNNCC	3 cut(s)	787, 1966, 2079
PspPI	GGNCC	4 cut(s)	655, 952, 1080, 1571
PstI	CTGCAG	3 cut(s)	1012, 2020, 2242
PsuI	RGATCY	2 cut(s)	1564, 1651
PvuII	CAGCTG	1 cut(s)	2021
RruI	TCGCGA	1 cut(s)	2181
RsaI	GTAC	6 cut(s)	228, 233, 787, 1389, 1845, 2220
RsaNI	GTAC	6 cut(s)	227, 232, 786, 1388, 1844, 2219
RseI	CAYNNNNRTG	4 cut(s)	254, 963, 1450, 1755
SacI	GAGCTC	2 cut(s)	972, 1542
SaqAI	TTAA	5 cut(s)	333, 1412, 1433, 2088, 2335
SatI	GCNGC	3 cut(s)	1008, 1278, 2019
Sau96I	GGNCC	4 cut(s)	655, 952, 1080, 1571
SchI	GAGTC	4 cut(s)	453, 625, 746, 1069
ScrFI	CCNGG	5 cut(s)	1174, 1211, 1371, 1853, 2006
SduI	GDGCHC	4 cut(s)	628, 972, 1542, 1793
SfaNI	GCATC	8 cut(s)	226, 249, 605, 871, 1003, 1101, 1855, 2050
SfcI	CTRYAG	5 cut(s)	454, 1008, 1192, 2016, 2238
SinI	GGWCC	2 cut(s)	655, 1571
SmiMI	CAYNNNNRTG	4 cut(s)	254, 963, 1450, 1755
SmlI	CTYRAG	4 cut(s)	332, 762, 1023, 2321
SmoI	CTYRAG	4 cut(s)	332, 762, 1023, 2321
SpeI	ACTAGT	1 cut(s)	575
Sse9I	AATT	4 cut(s)	63, 983, 1288, 1463
SseBI	AGGCCT	1 cut(s)	1209
SspI	AATATT	1 cut(s)	1623
SspMI	CTAG	4 cut(s)	576, 933, 2303, 2312
SstI	GAGCTC	2 cut(s)	972, 1542
StuI	AGGCCT	1 cut(s)	1209
StyD4I	CCNGG	5 cut(s)	1172, 1209, 1369, 1851, 2004
StyI	CCWWGG	2 cut(s)	658, 1605
TaaI	ACNGT	8 cut(s)	231, 477, 799, 1196, 1255, 1305, 1681, 1969
TaiI	ACGT	3 cut(s)	1450, 1897, 2188
TaqI	TCGA	1 cut(s)	1897
TasI	AATT	4 cut(s)	63, 983, 1288, 1463
TatI	WGTACW	3 cut(s)	226, 1387, 1843
TfiI	GAWTC	4 cut(s)	211, 665, 773, 1518
Tru1I	TTAA	5 cut(s)	333, 1412, 1433, 2088, 2335
Tru9I	TTAA	5 cut(s)	333, 1412, 1433, 2088, 2335
TseFI	GTSAC	4 cut(s)	562, 588, 749, 2051
TseI	GCWGC	3 cut(s)	1007, 1277, 2018
Tsp45I	GTSAC	4 cut(s)	562, 588, 749, 2051
TspGWI	ACGGA	1 cut(s)	1677
Vha464I	CTTAAG	1 cut(s)	332
VpaK11BI	GGWCC	2 cut(s)	655, 1571
XagI	CCTNNNNNAGG	1 cut(s)	1661
XapI	RAATTY	1 cut(s)	1463
XceI	RCATGY	2 cut(s)	283, 494
XmnI	GAANNNNTTC	4 cut(s)	669, 798, 1887, 2187
XspI	CTAG	4 cut(s)	576, 933, 2303, 2312
Zsp2I	ATGCAT	2 cut(s)	194, 1324

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.