Rw2G020850

Mitochondrial protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001471
Physical Location & Seq
Forward (+)
28576817 .. 28577853
1037 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G020850.1

Sequence Viewer

Length: 747 bp
ATGAATGAAGAGATTGATGCTTTGAAGAAACAAGGTACATGGCAACTTGTTCCATTCCCTGAAGGTAAAAATGTTGTTGGAAGTAAATGGGTGTATAAAATAAAAAAGAATCCAGATGGGAGTGTTTCAAGATATAAAGCAAGATTGGTTGCTCAGGGATACAGTCAAGAAAAAGGCTTGGATTATGATGAAACTTTTAGTCCAGTTGTAAGGCATAGCACAGTGAGAATTATTCTTTCTCTAGCTGCAATGTATAGCTGGGAATTATGGCAACTTGATGTCAAAAATGCTTTTCTGCATGGTGATCTTAAAGAAGAAGTATATATGCATCAGCCTCATGGTTTTGTTGATCCAATTCATTCCAATCATTACAAAGACAAGGGTATCTTTTTATCACAGGAGACATATGCAAAGGAGTTGATAGCCAAAGCTGGTTTAGAAACTTGCAAAGAGTGTAATACTCCATGTTTGCCTCATTTTCAGCTTCTCAAAAATGAAGGTACACCTCTGTCAAATCCTACTCTCTATAGGAGCATTGTTGGTGCATTACAATACTTGACATTTACCCGGCCTGATATAGCTTATGCTGTAAACACTGTATGTCAATTCATGTCCACTCCAACTGATGTACAATTTGCATATGTTGAGAGAATCTTGAGATATTTGCAAGGTACTATGTCTAGAGGGCTGTTTTACAAGTTTGGTAATAGTGTTGCTTACATCAATGCTTTCTGTGATGCTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2

Protein Analysis

248

Amino Acids

28.31

Weight (kDa)

7.64

Isoelectric Point (pI)

39.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_2 PF07727 13 - 117 2.3e-36 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 344
AcuI CTGAAG 1 cut(s) 81
AfaI GTAC 4 cut(s) 37, 502, 630, 673
AgsI TTSAA 2 cut(s) 25, 129
AluBI AGCT 5 cut(s) 245, 258, 431, 484, 581
AluI AGCT 5 cut(s) 245, 258, 431, 484, 581
Alw26I GTCTC 1 cut(s) 395
AlwI GGATC 1 cut(s) 344
AoxI GGCC 1 cut(s) 569
ApeKI GCWGC 1 cut(s) 245
AsuC2I CCSGG 1 cut(s) 568
AsuHPI GGTGA 1 cut(s) 314
BaeI ACNNNNGTAYC 2 cut(s) 367, 400
BbvI GCAGC 1 cut(s) 232
BccI CCATC 1 cut(s) 110
BciVI GTATCC 1 cut(s) 152
BcnI CCSGG 1 cut(s) 568
BcoDI GTCTC 1 cut(s) 395
BfaI CTAG 2 cut(s) 242, 681
BfmI CTRYAG 1 cut(s) 526
BfuI GTATCC 1 cut(s) 152
BisI GCNGC 1 cut(s) 246
BlsI GCNGC 1 cut(s) 247
Bme1390I CCNGG 1 cut(s) 568
BmrFI CCNGG 1 cut(s) 568
BmsI GCATC 3 cut(s) 7, 337, 727
Bpu10I CCTNAGC 1 cut(s) 153
BpuEI CTTGAG 1 cut(s) 676
BpuMI CCSGG 1 cut(s) 568
Bse1I ACTGG 1 cut(s) 203
Bse3DI GCAATG 1 cut(s) 255
BseMI GCAATG 1 cut(s) 255
BseMII CTCAG 1 cut(s) 167
BseNI ACTGG 1 cut(s) 203
BseXI GCAGC 1 cut(s) 232
BseYI CCCAGC 1 cut(s) 258
BshFI GGCC 1 cut(s) 571
BsiSI CCGG 1 cut(s) 568
BsmAI GTCTC 1 cut(s) 395
BsnI GGCC 1 cut(s) 571
Bsp1407I TGTACA 1 cut(s) 628
Bsp143I GATC 2 cut(s) 304, 349
BspANI GGCC 1 cut(s) 571
BspCNI CTCAG 1 cut(s) 166
BspPI GGATC 1 cut(s) 344
BsrDI GCAATG 1 cut(s) 255
BsrGI TGTACA 1 cut(s) 628
BsrI ACTGG 1 cut(s) 203
BssMI GATC 2 cut(s) 304, 349
Bst4CI ACNGT 3 cut(s) 164, 223, 598
Bst6I CTCTTC 1 cut(s) 3
BstAUI TGTACA 1 cut(s) 628
BstDEI CTNAG 1 cut(s) 153
BstKTI GATC 2 cut(s) 307, 352
BstMAI GTCTC 1 cut(s) 395
BstMBI GATC 2 cut(s) 304, 349
BstSCI CCNGG 1 cut(s) 566
BstSFI CTRYAG 1 cut(s) 526
BstV1I GCAGC 1 cut(s) 232
BsuI GTATCC 1 cut(s) 152
BsuRI GGCC 1 cut(s) 571
BtsIMutI CAGTG 2 cut(s) 228, 594
Csp6I GTAC 4 cut(s) 36, 501, 629, 672
CviAII CATG 5 cut(s) 39, 299, 338, 465, 610
CviQI GTAC 4 cut(s) 36, 501, 629, 672
DdeI CTNAG 1 cut(s) 153
DpnI GATC 2 cut(s) 306, 351
DpnII GATC 2 cut(s) 304, 349
Eam1104I CTCTTC 1 cut(s) 3
EarI CTCTTC 1 cut(s) 3
Eco57I CTGAAG 1 cut(s) 81
EcoT22I ATGCAT 1 cut(s) 330
FaeI CATG 5 cut(s) 42, 302, 341, 468, 613
FalI AAGNNNNNCTT 2 cut(s) 371, 403
FatI CATG 5 cut(s) 38, 298, 337, 464, 609
FauNDI CATATG 2 cut(s) 406, 640
Fnu4HI GCNGC 1 cut(s) 246
Fsp4HI GCNGC 1 cut(s) 246
FspBI CTAG 2 cut(s) 242, 681
GluI GCNGC 1 cut(s) 246
GsaI CCCAGC 1 cut(s) 262
HaeIII GGCC 1 cut(s) 571
HapII CCGG 1 cut(s) 568
Hin1II CATG 5 cut(s) 42, 302, 341, 468, 613
HinfI GANTC 2 cut(s) 109, 651
HpaII CCGG 1 cut(s) 568
HphI GGTGA 1 cut(s) 314
Hpy166II GTNNAC 3 cut(s) 503, 592, 615
Hpy188III TCNNGA 5 cut(s) 113, 129, 167, 655, 681
Hpy8I GTNNAC 3 cut(s) 503, 592, 615
HpyAV CCTTC 2 cut(s) 56, 491
HpyCH4III ACNGT 3 cut(s) 164, 223, 598
HpyCH4V TGCA 8 cut(s) 248, 298, 328, 410, 447, 545, 638, 667
HpyF3I CTNAG 1 cut(s) 153
Hsp92II CATG 5 cut(s) 42, 302, 341, 468, 613
Kzo9I GATC 2 cut(s) 304, 349
LmnI GCTCC 1 cut(s) 531
LpnPI CCDG 9 cut(s) 72, 126, 140, 216, 244, 383, 417, 581, 585
Lsp1109I GCAGC 1 cut(s) 232
LweI GCATC 3 cut(s) 7, 337, 727
MaeI CTAG 2 cut(s) 242, 681
MalI GATC 2 cut(s) 306, 351
MboI GATC 2 cut(s) 304, 349
MboII GAAGA 3 cut(s) 20, 37, 326
MluCI AATT 5 cut(s) 228, 263, 354, 605, 632
MmeI TCCRAC 2 cut(s) 58, 644
MnlI CCTC 4 cut(s) 345, 483, 516, 677
Mph1103I ATGCAT 1 cut(s) 330
MseI TTAA 1 cut(s) 309
MspI CCGG 1 cut(s) 568
MspR9I CCNGG 1 cut(s) 568
NciI CCSGG 1 cut(s) 568
NdeI CATATG 2 cut(s) 406, 640
NdeII GATC 2 cut(s) 304, 349
NlaIII CATG 5 cut(s) 42, 302, 341, 468, 613
NsiI ATGCAT 1 cut(s) 330
PfeI GAWTC 2 cut(s) 109, 651
PkrI GCNGC 1 cut(s) 247
PspFI CCCAGC 1 cut(s) 258
RsaI GTAC 4 cut(s) 37, 502, 630, 673
RsaNI GTAC 4 cut(s) 36, 501, 629, 672
SaqAI TTAA 1 cut(s) 309
SatI GCNGC 1 cut(s) 246
Sau3AI GATC 2 cut(s) 304, 349
ScrFI CCNGG 1 cut(s) 568
SfaNI GCATC 3 cut(s) 7, 337, 727
SfcI CTRYAG 1 cut(s) 526
SmlI CTYRAG 1 cut(s) 655
SmoI CTYRAG 1 cut(s) 655
Sse9I AATT 5 cut(s) 228, 263, 354, 605, 632
SspMI CTAG 2 cut(s) 242, 681
StyD4I CCNGG 1 cut(s) 566
TaaI ACNGT 3 cut(s) 164, 223, 598
TasI AATT 5 cut(s) 228, 263, 354, 605, 632
TatI WGTACW 1 cut(s) 628
TfiI GAWTC 2 cut(s) 109, 651
Tru1I TTAA 1 cut(s) 309
Tru9I TTAA 1 cut(s) 309
TscAI CASTG 2 cut(s) 228, 601
TseI GCWGC 1 cut(s) 245
TspDTI ATGAA 6 cut(s) 17, 21, 204, 347, 510, 598
TspRI CASTG 2 cut(s) 228, 601
XbaI TCTAGA 1 cut(s) 680
XspI CTAG 2 cut(s) 242, 681
Zsp2I ATGCAT 1 cut(s) 330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.