RchiOBHm_Chr2g0119341

transposition

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0001471
Physical Location & Seq
Forward (+)
31731177 .. 31736533
5357 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ49208

Sequence Viewer

Length: 4596 bp
ATGGCGTTTCCTACTCAAGTGTGCAACACCATCTCTGTTCGTTTGGATGAGACAAATTATCCTACCTGGGTTTTTCATATGCAACATCATCTCAGAGGGCATGGTCTTCTGAAATTTGTGGATGGTTCTCATCCTTGTCCATCACAATTTCTTGTTGAAGATGATGGTTCTCTAACTAATAACTCCGCTGCTCGTGAAAAATGGATGGAGCAAGATAGTGCTCTTATCTCTATGTTGATCAATACCTTGTCACCTGAGGCACTTACCCTTGTCACTGGCTGCTCTTCATCTATGGAGGTATGGTTGATTCTCAAACAAAGATATGCAACTGTTTCAGAAGCACATGTCATGCAATTAAAATCTACACTGCAAAATATTCAGAAGGGTAGTGATTCAATTGAAAAATACCTGTTGAAATTTAAGACTGTTAGAGATCAACTTGCTGTTCTTGGTGTCAAGATGTCCGATCAAGATGTGAAAGTGTTAATTCTAGCTGGTTTACCAAGTGAATATGGCCACACTAGACAAATCATAAGGGGAAAGAACAATATTGATTTGGAAGAAGTTAGGTCATTACTACTATCTGCTGAAAGTGAGATTGAATTGGAAAACAAGTCATCACCTTTGATTCAGCTCACTGCTCTGCTAAGTCAAACTGTTGCTGCTGCATCCACTACTGTCCCTGCTCAAAATGATAAGCCAAACCCTCTTGTTCAACTTACTGCTTTACTTGCTCAATCTGTTTTGGGAAATACTAATGCTATGCAAGGAATGAATTGTAACAATGCTGCTGCATCTACTACCACAACACACATGAATGCCTATACTGGACCTGTGAATTCTGCAAGTCAAACTGGTTTTATGGTACCTCCAAGCAATGGAACACCTCAATCTGGGTTCTTGGCAAACAATGGTGGCATGCAGGGTAACAACACAACTCAGCACTCTCAGGGTTTCTCACAAAACAATGGCTACAACAATAATGGAGGGTCTTACTCTGATAATCAAGGTAGTTATCCACAGTTTCCTGCTCAGAGAAACTTTAGCAATAACAACAACAATAACCAGAGGTACTCAAGGGGCAACAACCCGAATCAGAACTACAACAGCCCACTTATATGTCAGATATGTGACAAACCTGGACATGGAGCATGGAAATGTCATCAAAGAAATCCTCAAACTCAGAATGATGGGTCAAGCTCAACCATAGAGTGTCAAATCTGCAAGAAACCTGGACACATTGCCAAAACATGCAAATACAGGATTCCTAACAACTCCAACAATCACACTGGTTCTTCAGCTATGGTTGCTTGTGCAGAATCAAATCATGAAGTCTGGCTTGTTGACTCAGGGGCAACCAACCACATGACATCAGATCTTCAAAACCTGCAGAATGTCTCTAGCTACAACTCAAATGATGGTGTGCAGATTGAATGTGACAAGGTGACGGGCAACATTTTATATGATGGACTGTGTGATCAAGGCCTTTATCCCATTCCAGTCTATGTTGCTTCTAAGCTTCTTCCAGCCTCTAAACCTTCAGCTTTTCTAGGAAACAAAGTCTCACATTCTATCTGGCACAAAAGATTGGGTCACCCTTCTAATGTCGTTGTCTCTAGTATGTTGTCTAAAGCTCATATTTCTGTAGAACCTGATAAGTGTACATCATTGTGTGAATCATGTTTGTCTGGAAAGTTTACCAAACTTCCTTTCTCGTTGTCTTCAGAAAAGTCTAGTATACCTTTTGAGATCATACACTCTGATCTATGGGGGCCTTCCCCTAGTATGTCTTTTGAAGGCTTTAAGTACTATTTTACTCTCATAGATGAATGTACCAGATACACATGGATTTTTCCATTACGAAATAAAAGAGAAGCTTTTCCATTATTCCAAGTGTTTTGTGCCTTTATTGCAAATCAGTATAGTGCATCTGTTAAAGTACTCCAAACAGATGGGGGGGGAGAGTATACCAGTCATATCTTTAAAGAGTTTCTAGCCAAAAATGGGATTTCTCACCAATTATCTTGTCCATATACTCCACAGCAAAATGGCCTTGCAGAGAGGAAAAATCGACACATTGTTGAAACTGCTCTCACTCTCTTAAACAATGCTTCACTTCCACCAAGGTTTTGGTATCATGCATGTGCCATTTCTGTGTATCTTATCAACAGAATGCCTTGTACTACCCTCTCCATGAAATCACCATTTGAACTTCTCTTCAAAAAGCTTCCTCCCATTGACCACATTAGAGTATTTGGCTGTACCTGTTATCCACTCCTCAAACCATATAACTCCAATAAGCTTCAACCGAAGACAGCTCCATGCATATTCATTGGTTTCGCTATAGGCTATAAGGGCTTTATTTGTTACAGTCCAGTTTCCCAAAAAAGTATCATATCCAGACATGTCATTTTTGATGAGTCCAAGTTCTATTTCAAATCTATCCCAACTCATTCTCAGTCCAATGTTAATCACACCAATACTCAGTCTTTGCCTTGTTCTATGTCTACACCATTGCACACTAACCTTGTCACACAGTCACCATCAGTTCAACTGCCTCACCTTGTCCTTCATCCATCTCCACCAGCAACCTGCACTACTACATCAGATACTAGTATACCAGAATCAAGTTCCTCAAGCACAAATATTGATCACATCCCTACTGACAACACCATTGATTGCACACCTGCATCCATTCCAATGCATGACACTATCGTTCCAGTGGAATTTATTGATACTCCATACATACCAATCTCAGACATTGTTCTTGATATAAATCCTCCTAGTCCAGATTCACACAACAGGATGATCAATTTTCTACCTCCATCCAACCTCACAGTGAATGATCATCCCATGATGACAAGAAGTAAAAATGGCATCTACAAGAAGAAAGTCTTCTATGCAGCAAATATTGCTGCTACTGTTTCAGCAAATGAAGAACCTACCTCTCACAAGCAAGCAATGAAGATTCCCGAATGGAAGCATGCAATGCAGGATGAGTATGATGCCTTACTAAATCAAGGGACCTGGATACTAGTCCCACCACAACAGCAAAAAAATATTGTGAGCTGCAAGTGGGTTTTTAAATTGAAAAAGAACGCTGATGGCACCATTGCACGACATAAGGCCAGGTTAGTGGCAAGGGGGTTTAGTCAAGAAGAAGGTACAGATTATGATGAAACATTCAGCCCTGTGGTTAGGCATACTACAGTTAGGTTGTTCCTTGCTCTTGCTGCTCAATATCAATGGAGACTTTACCAAATGGATGTAAAAAATGCTTTTTTACATGGCGTTCTCAAAGAAGAAATATACATGGCTCAACCACCTGGTTTTGAAAACCCTTCTTGCCCTACACATGTTTGCAAACTCATTAAATCATTATATGGTTTAAAACAGGCCCCTCGAGCATGGAATGAGCGATTCACCTCTTTTCTACCATCTATTGGATTCAAGGGCTCCTATTCTGATCCTTCCTTATTCATCAAGTCATCTGCTCAAGGTATGGTCTATTTGCTATTGTATGTGGATGACATTATCTTAACTGGAAGCAATGAAAGTGAAATCCACTCTGTCAAAATGGCTTTACAACTTGAGTTTGAAATGAAGGACCTAGGTCTTTTGCATTATTTCCTGGGTTTACAAATCGATTATTTGCCATCTGGAGGGATTTTTGTTTCTCAATCTAAGTATGCAGATGATATTCTTCTAAAAGCTGGGATGATTGGCAGCAAAGTGTGTAGTACTCCATGCCTTCCTTATGCCAAACTACTAAGAGATGAGGGTCCTCCTTATGCAGATGTCACACACTATAGAAGTTTGGTTGGTTGTTTACAATACTTGACTTTCACTAGACCTGACTTGGCTTATAGTGTAAACACTGTATGTCAGTTTATGCAAAACCCCACTGAGGCTCACTATGCTGCAGTTAAAAGAATACTCCGATATCTCCTTGGCACTACCAATTATGGTCTCACTTACAGGTCAGCTCCGTTTGAACTTAGAGCATTCTCCGATGCAGATTGGGCCGGGGACCCCAATGATAGACGATCAACAACAGGATTTGTCATCTATATTGGTAACTGTCCTATCTCTTGGTGCTCAAAGAAACAGCACTCTGTGTCTAGATCATCTACAGAAGCCGAATACAGGGCAATGGCTGATACTGCCTCAGAAATTTTTTGGTTAAGACATCTTCTTGCGGACATCTCTATCCCATTACCTTATGCACCTGTTTTGCATTGTGACAATGTGTCTGCTCTAGCCTTGGCCTCAAATCCAGTTCATAAATCCAAATGCAAGCATGTTGAAGTCGACGTTCATTTCACTAGGGAAAAAGTGATGAGAGGAGAGTTGACACTGCAGTTTGTTCCATCCCTTGATCAGTTTGCCGATATCTTTACTAAGGGACTCAGTACAGCTCAATTCAAGCACTTATGCTGCAATCTAGTGCTTGGAGCTCCCACCACTAGCTTGAGGGGGGATGATAAAGTGAAGAATGTAGCTCATCAAGGTCTGCATAATCAAGGATTAGACTACACAGTTCAAGAACAAATATCCAGTCAACCAATTGAAGGCTCAGGATCTGATCTCAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HTH_21 rve

Protein Analysis

1531

Amino Acids

169.92

Weight (kDa)

7.87

Isoelectric Point (pI)

46.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotran_gag_2 PF14223 27 - 199 3.5e-24 gag-polypeptide of LTR copia-type
gag_pre-integrs PF13976 515 - 565 2.2e-09 GAG-pre-integrase domain
rve PF00665 580 - 680 1.4e-13 Integrase core domain
SH3_retrovirus PF25597 754 - 814 2.6e-19 Retroviral polymerase SH3-like domain
RVT_2 PF07727 1009 - 1251 1.6e-71 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2695
Acc36I ACCTGC 3 cut(s) 1395, 2600, 2695
Acc65I GGTACC 1 cut(s) 865
AccB1I GGYRCC 2 cut(s) 865, 3107
AccB7I CCANNNNNTGG 2 cut(s) 878, 3443
AccI GTMKAC 5 cut(s) 1738, 1967, 2507, 2617, 4311
AciI CCGC 2 cut(s) 186, 4199
AclWI GGATC 2 cut(s) 3461, 4588
AcoI YGGCCR 1 cut(s) 514
AcsI RAATTY 5 cut(s) 113, 416, 838, 2726, 4173
AcuI CTGAAG 3 cut(s) 1281, 1524, 1707
AdeI CACNNNGTG 1 cut(s) 4117
AfiI CCNNNNNNNGG 9 cut(s) 878, 893, 1145, 2004, 3443, 3662, 3907, 4146, 4571
AflIII ACRYGT 3 cut(s) 343, 2404, 3355
AhdI GACNNNNNGTC 1 cut(s) 4249
AhlI ACTAGT 2 cut(s) 2612, 3034
AjnI CCWGG 7 cut(s) 65, 1138, 1231, 3026, 3128, 3325, 3630
AjuI GAANNNNNNNTTGG 4 cut(s) 1694, 1726, 2417, 2449
Alw21I GWGCWC 3 cut(s) 223, 4100, 4459
Alw26I GTCTC 6 cut(s) 44, 1402, 1567, 1618, 3244, 3974
AlwI GGATC 2 cut(s) 3461, 4588
AlwNI CAGNNNCTG 1 cut(s) 4583
Ama87I CYCGRG 1 cut(s) 3402
AoxI GGCC 8 cut(s) 514, 1483, 1772, 2050, 3126, 3396, 4023, 4266
ApoI RAATTY 5 cut(s) 113, 416, 838, 2726, 4173
ArsI GACNNNNNNTTYG 2 cut(s) 4543, 4575
Asp700I GAANNNNTTC 2 cut(s) 1878, 2894
Asp718I GGTACC 1 cut(s) 865
AspA2I CCTAGG 1 cut(s) 3610
AspS9I GGNCC 8 cut(s) 830, 1772, 3024, 3397, 3607, 3782, 4023, 4030
AsuC2I CCSGG 1 cut(s) 4027
AsuHPI GGTGA 9 cut(s) 243, 612, 1456, 1586, 2006, 2193, 2532, 2552, 3415
AvaI CYCGRG 1 cut(s) 3402
AvaII GGWCC 5 cut(s) 830, 3024, 3607, 3782, 4030
AvrII CCTAGG 1 cut(s) 3610
AxyI CCTNAGG 1 cut(s) 255
BalI TGGCCA 1 cut(s) 516
BanI GGYRCC 2 cut(s) 865, 3107
BanII GRGCYC 2 cut(s) 3458, 4459
BauI CACGAG 1 cut(s) 192
BbsI GAAGAC 4 cut(s) 98, 1713, 2318, 2887
Bbv12I GWGCWC 3 cut(s) 223, 4100, 4459
BciT130I CCWGG 7 cut(s) 67, 1140, 1233, 3028, 3130, 3327, 3632
BciVI GTATCC 1 cut(s) 3024
BclI TGATCA 6 cut(s) 237, 1477, 2650, 2808, 2845, 4378
BcnI CCSGG 1 cut(s) 4027
BcoDI GTCTC 6 cut(s) 44, 1402, 1567, 1618, 3244, 3974
BcuI ACTAGT 2 cut(s) 2612, 3034
BfmI CTRYAG 8 cut(s) 1388, 1644, 2343, 3207, 3808, 3921, 4131, 4358
BfuAI ACCTGC 3 cut(s) 1395, 2600, 2695
BfuI GTATCC 1 cut(s) 3024
BglII AGATCT 1 cut(s) 1375
BlnI CCTAGG 1 cut(s) 3610
BmcAI AGTACT 3 cut(s) 1808, 1941, 3742
Bme1390I CCNGG 8 cut(s) 67, 1140, 1233, 3028, 3130, 3327, 3632, 4027
Bme18I GGWCC 5 cut(s) 830, 3024, 3607, 3782, 4030
BmeRI GACNNNNNGTC 1 cut(s) 4249
BmeT110I CYCGRG 1 cut(s) 3402
BmgT120I GGNCC 8 cut(s) 830, 1772, 3024, 3397, 3607, 3782, 4023, 4030
BmiI GGNNCC 9 cut(s) 867, 1773, 3025, 3109, 3399, 3457, 3783, 4031, 4032
BmrFI CCNGG 8 cut(s) 67, 1140, 1233, 3028, 3130, 3327, 3632, 4027
BmsI GCATC 7 cut(s) 677, 803, 1937, 2699, 2886, 2995, 4003
BoxI GACNNNNGTC 1 cut(s) 3612
BpiI GAAGAC 4 cut(s) 98, 1713, 2318, 2887
BpmI CTGGAG 1 cut(s) 3681
Bpu10I CCTNAGC 1 cut(s) 4576
BpuEI CTTGAG 5 cut(s) 1060, 2620, 3480, 3611, 4492
BpuMI CCSGG 1 cut(s) 4027
Bsa29I ATCGAT 1 cut(s) 3645
BsaBI GATNNNNATC 1 cut(s) 2775
BsaI GGTCTC 1 cut(s) 3974
BsaJI CCNNGG 7 cut(s) 66, 2123, 3610, 3631, 3949, 4026, 4263
BsaXI ACNNNNNCTCC 2 cut(s) 287, 317
Bsc4I CCNNNNNNNGG 9 cut(s) 878, 893, 1145, 2004, 3443, 3662, 3907, 4146, 4571
Bse21I CCTNAGG 1 cut(s) 255
Bse3DI GCAATG 8 cut(s) 883, 1239, 2513, 2967, 2994, 3111, 3556, 4158
Bse8I GATNNNNATC 1 cut(s) 2775
BseBI CCWGG 7 cut(s) 67, 1140, 1233, 3028, 3130, 3327, 3632
BseCI ATCGAT 1 cut(s) 3645
BseDI CCNNGG 7 cut(s) 66, 2123, 3610, 3631, 3949, 4026, 4263
BseJI GATNNNNATC 1 cut(s) 2775
BseLI CCNNNNNNNGG 9 cut(s) 878, 893, 1145, 2004, 3443, 3662, 3907, 4146, 4571
BseMI GCAATG 8 cut(s) 883, 1239, 2513, 2967, 2994, 3111, 3556, 4158
BseRI GAGGAG 2 cut(s) 2267, 4359
BseYI CCCAGC 1 cut(s) 3713
BsgI GTGCAG 3 cut(s) 1335, 1445, 2578
BshFI GGCC 8 cut(s) 516, 1485, 1774, 2052, 3128, 3398, 4025, 4268
BshNI GGYRCC 2 cut(s) 865, 3107
BshVI ATCGAT 1 cut(s) 3645
BsiHKAI GWGCWC 3 cut(s) 223, 4100, 4459
BsiHKCI CYCGRG 1 cut(s) 3402
BsiSI CCGG 1 cut(s) 4026
BslFI GGGAC 5 cut(s) 665, 3023, 3037, 4043, 4419
BslI CCNNNNNNNGG 9 cut(s) 878, 893, 1145, 2004, 3443, 3662, 3907, 4146, 4571
BsmAI GTCTC 6 cut(s) 44, 1402, 1567, 1618, 3244, 3974
BsmFI GGGAC 5 cut(s) 665, 3023, 3037, 4043, 4419
BsmI GAATGC 3 cut(s) 823, 2178, 4004
BsnI GGCC 8 cut(s) 516, 1485, 1774, 2052, 3128, 3398, 4025, 4268
Bso31I GGTCTC 1 cut(s) 3974
BsoBI CYCGRG 1 cut(s) 3402
Bsp1286I GDGCHC 4 cut(s) 223, 3458, 4100, 4459
Bsp1407I TGTACA 1 cut(s) 1661
BspACI CCGC 2 cut(s) 186, 4199
BspANI GGCC 8 cut(s) 516, 1485, 1774, 2052, 3128, 3398, 4025, 4268
BspDI ATCGAT 1 cut(s) 3645
BspHI TCATGA 1 cut(s) 1327
BspLI GGNNCC 9 cut(s) 867, 1773, 3025, 3109, 3399, 3457, 3783, 4031, 4032
BspMAI CTGCAG 3 cut(s) 1392, 3925, 4362
BspMI ACCTGC 3 cut(s) 1395, 2600, 2695
BspPI GGATC 2 cut(s) 3461, 4588
BspQI GCTCTTC 1 cut(s) 289
BspT107I GGYRCC 2 cut(s) 865, 3107
BspTNI GGTCTC 1 cut(s) 3974
BsrDI GCAATG 8 cut(s) 883, 1239, 2513, 2967, 2994, 3111, 3556, 4158
BsrGI TGTACA 1 cut(s) 1661
BssECI CCNNGG 7 cut(s) 66, 2123, 3610, 3631, 3949, 4026, 4263
BssNAI GTATAC 3 cut(s) 1739, 1968, 2618
BssSI CACGAG 1 cut(s) 192
BssT1I CCWWGG 4 cut(s) 2123, 3610, 3949, 4263
Bst1107I GTATAC 3 cut(s) 1739, 1968, 2618
Bst2BI CACGAG 1 cut(s) 192
Bst2UI CCWGG 7 cut(s) 67, 1140, 1233, 3028, 3130, 3327, 3632
Bst6I CTCTTC 2 cut(s) 289, 2222
BstAPI GCANNNNNTGC 2 cut(s) 2912, 2989
BstAUI TGTACA 1 cut(s) 1661
BstC8I GCNNGC 4 cut(s) 920, 2958, 2985, 4298
BstEII GGTNACC 1 cut(s) 1592
BstMAI GTCTC 6 cut(s) 44, 1402, 1567, 1618, 3244, 3974
BstNI CCWGG 7 cut(s) 67, 1140, 1233, 3028, 3130, 3327, 3632
BstNSI RCATGY 8 cut(s) 347, 922, 1254, 2145, 2408, 2987, 3359, 4304
BstPAI GACNNNNGTC 1 cut(s) 3612
BstPI GGTNACC 1 cut(s) 1592
BstSCI CCNGG 8 cut(s) 65, 1138, 1231, 3026, 3128, 3325, 3630, 4025
BstSFI CTRYAG 8 cut(s) 1388, 1644, 2343, 3207, 3808, 3921, 4131, 4358
BstV2I GAAGAC 4 cut(s) 98, 1713, 2318, 2887
BstX2I RGATCY 2 cut(s) 1375, 4580
BstXI CCANNNNNNTGG 3 cut(s) 1952, 2130, 3136
BstYI RGATCY 2 cut(s) 1375, 4580
BstZ17I GTATAC 3 cut(s) 1739, 1968, 2618
Bsu15I ATCGAT 1 cut(s) 3645
Bsu36I CCTNAGG 1 cut(s) 255
BsuI GTATCC 1 cut(s) 3024
BsuRI GGCC 8 cut(s) 516, 1485, 1774, 2052, 3128, 3398, 4025, 4268
BsuTUI ATCGAT 1 cut(s) 3645
BtsI GCAGTG 3 cut(s) 365, 636, 4355
BtsIMutI CAGTG 9 cut(s) 273, 365, 636, 1287, 2727, 2844, 3876, 3903, 4355
BveI ACCTGC 3 cut(s) 1395, 2600, 2695
Cac8I GCNNGC 4 cut(s) 920, 2958, 2985, 4298
CaiI CAGNNNCTG 1 cut(s) 4583
CciI TCATGA 1 cut(s) 1327
Cfr13I GGNCC 8 cut(s) 830, 1772, 3024, 3397, 3607, 3782, 4023, 4030
ClaI ATCGAT 1 cut(s) 3645
CsiI ACCWGGT 1 cut(s) 3325
DraI TTTAAA 3 cut(s) 1984, 3085, 3390
DraIII CACNNNGTG 1 cut(s) 4117
DriI GACNNNNNGTC 1 cut(s) 4249
EaeI YGGCCR 1 cut(s) 514
Eam1104I CTCTTC 2 cut(s) 289, 2222
Eam1105I GACNNNNNGTC 1 cut(s) 4249
EarI CTCTTC 2 cut(s) 289, 2222
Ecl136II GAGCTC 1 cut(s) 4457
Eco130I CCWWGG 4 cut(s) 2123, 3610, 3949, 4263
Eco147I AGGCCT 1 cut(s) 1485
Eco24I GRGCYC 2 cut(s) 3458, 4459
Eco31I GGTCTC 1 cut(s) 3974
Eco32I GATATC 2 cut(s) 3944, 4393
Eco47I GGWCC 5 cut(s) 830, 3024, 3607, 3782, 4030
Eco53kI GAGCTC 1 cut(s) 4457
Eco57I CTGAAG 3 cut(s) 1281, 1524, 1707
Eco81I CCTNAGG 1 cut(s) 255
Eco88I CYCGRG 1 cut(s) 3402
Eco91I GGTNACC 1 cut(s) 1592
EcoICRI GAGCTC 1 cut(s) 4457
EcoO109I RGGNCCY 6 cut(s) 1772, 3024, 3397, 3607, 3782, 4030
EcoO65I GGTNACC 1 cut(s) 1592
EcoRI GAATTC 1 cut(s) 838
EcoRII CCWGG 7 cut(s) 65, 1138, 1231, 3026, 3128, 3325, 3630
EcoRV GATATC 2 cut(s) 3944, 4393
EcoT14I CCWWGG 4 cut(s) 2123, 3610, 3949, 4263
EcoT22I ATGCAT 3 cut(s) 2143, 2327, 2706
EcoT38I GRGCYC 2 cut(s) 3458, 4459
ErhI CCWWGG 4 cut(s) 2123, 3610, 3949, 4263
FalI AAGNNNNNCTT 6 cut(s) 1323, 1355, 1861, 1893, 2879, 2911
FaqI GGGAC 5 cut(s) 665, 3023, 3037, 4043, 4419
FauNDI CATATG 1 cut(s) 78
FbaI TGATCA 6 cut(s) 237, 1477, 2650, 2808, 2845, 4378
FblI GTMKAC 5 cut(s) 1738, 1967, 2507, 2617, 4311
FriOI GRGCYC 2 cut(s) 3458, 4459
GsaI CCCAGC 1 cut(s) 3717
GsuI CTGGAG 1 cut(s) 3681
HaeIII GGCC 8 cut(s) 516, 1485, 1774, 2052, 3128, 3398, 4025, 4268
HapII CCGG 1 cut(s) 4026
HincII GTYRAC 4 cut(s) 1345, 4312, 4353, 4562
HindII GTYRAC 4 cut(s) 1345, 4312, 4353, 4562
HindIII AAGCTT 4 cut(s) 1517, 1875, 2225, 2300
HpaII CCGG 1 cut(s) 4026
HphI GGTGA 9 cut(s) 243, 612, 1456, 1586, 2006, 2193, 2532, 2552, 3415
Hpy99I CGWCG 1 cut(s) 4316
HpyCH4IV ACGT 1 cut(s) 4314
HpySE526I ACGT 1 cut(s) 4314
KflI GGGWCCC 1 cut(s) 4030
KpnI GGTACC 1 cut(s) 869
Ksp22I TGATCA 6 cut(s) 237, 1477, 2650, 2808, 2845, 4378
LguI GCTCTTC 1 cut(s) 289
LmnI GCTCC 7 cut(s) 208, 1148, 2323, 3461, 3991, 4454, 4462
LweI GCATC 7 cut(s) 677, 803, 1937, 2699, 2886, 2995, 4003
MabI ACCWGGT 1 cut(s) 3325
MaeII ACGT 1 cut(s) 4314
MfeI CAATTG 2 cut(s) 396, 4566
MflI RGATCY 2 cut(s) 1375, 4580
MhlI GDGCHC 4 cut(s) 223, 3458, 4100, 4459
MlsI TGGCCA 1 cut(s) 516
MluNI TGGCCA 1 cut(s) 516
MlyI GAGTC 3 cut(s) 1340, 2429, 4401
MmeI TCCRAC 2 cut(s) 1302, 2853
Mox20I TGGCCA 1 cut(s) 516
Mph1103I ATGCAT 3 cut(s) 2143, 2327, 2706
MroXI GAANNNNTTC 2 cut(s) 1878, 2894
MscI TGGCCA 1 cut(s) 516
MslI CAYNNNNRTG 7 cut(s) 816, 1117, 1156, 1669, 2142, 2153, 2699
Msp20I TGGCCA 1 cut(s) 516
MspA1I CMGCKG 1 cut(s) 188
MspI CCGG 1 cut(s) 4026
MspR9I CCNGG 8 cut(s) 67, 1140, 1233, 3028, 3130, 3327, 3632, 4027
MunI CAATTG 2 cut(s) 396, 4566
Mva1269I GAATGC 3 cut(s) 823, 2178, 4004
MvaI CCWGG 7 cut(s) 67, 1140, 1233, 3028, 3130, 3327, 3632
NciI CCSGG 1 cut(s) 4027
NdeI CATATG 1 cut(s) 78
NlaIV GGNNCC 9 cut(s) 867, 1773, 3025, 3109, 3399, 3457, 3783, 4031, 4032
NsiI ATGCAT 3 cut(s) 2143, 2327, 2706
NspI RCATGY 8 cut(s) 347, 922, 1254, 2145, 2408, 2987, 3359, 4304
PaeI GCATGC 2 cut(s) 922, 2987
PaeR7I CTCGAG 1 cut(s) 3402
PagI TCATGA 1 cut(s) 1327
PaqCI CACCTGC 1 cut(s) 2695
PceI AGGCCT 1 cut(s) 1485
PciI ACATGT 3 cut(s) 343, 2404, 3355
PciSI GCTCTTC 1 cut(s) 289
PctI GAATGC 3 cut(s) 823, 2178, 4004
PdmI GAANNNNTTC 2 cut(s) 1878, 2894
PflMI CCANNNNNTGG 2 cut(s) 878, 3443
PleI GAGTC 3 cut(s) 1340, 2428, 4401
PpsI GAGTC 3 cut(s) 1340, 2428, 4401
PpuMI RGGWCCY 4 cut(s) 3024, 3607, 3782, 4030
PscI ACATGT 3 cut(s) 343, 2404, 3355
PshAI GACNNNNGTC 1 cut(s) 3612
Psp124BI GAGCTC 1 cut(s) 4459
Psp5II RGGWCCY 4 cut(s) 3024, 3607, 3782, 4030
Psp6I CCWGG 7 cut(s) 65, 1138, 1231, 3026, 3128, 3325, 3630
PspEI GGTNACC 1 cut(s) 1592
PspFI CCCAGC 1 cut(s) 3713
PspGI CCWGG 7 cut(s) 65, 1138, 1231, 3026, 3128, 3325, 3630
PspN4I GGNNCC 9 cut(s) 867, 1773, 3025, 3109, 3399, 3457, 3783, 4031, 4032
PspPI GGNCC 8 cut(s) 830, 1772, 3024, 3397, 3607, 3782, 4023, 4030
PspPPI RGGWCCY 4 cut(s) 3024, 3607, 3782, 4030
PspXI VCTCGAGB 1 cut(s) 3402
PstI CTGCAG 3 cut(s) 1392, 3925, 4362
PstNI CAGNNNCTG 1 cut(s) 4583
PsuI RGATCY 2 cut(s) 1375, 4580
RseI CAYNNNNRTG 7 cut(s) 816, 1117, 1156, 1669, 2142, 2153, 2699
SacI GAGCTC 1 cut(s) 4459
SalI GTCGAC 1 cut(s) 4310
SapI GCTCTTC 1 cut(s) 289
Sau96I GGNCC 8 cut(s) 830, 1772, 3024, 3397, 3607, 3782, 4023, 4030
ScaI AGTACT 3 cut(s) 1808, 1941, 3742
SchI GAGTC 3 cut(s) 1340, 2429, 4401
ScrFI CCNGG 8 cut(s) 67, 1140, 1233, 3028, 3130, 3327, 3632, 4027
SduI GDGCHC 4 cut(s) 223, 3458, 4100, 4459
SexAI ACCWGGT 1 cut(s) 3325
SfaNI GCATC 7 cut(s) 677, 803, 1937, 2699, 2886, 2995, 4003
SfcI CTRYAG 8 cut(s) 1388, 1644, 2343, 3207, 3808, 3921, 4131, 4358
Sfr274I CTCGAG 1 cut(s) 3402
SinI GGWCC 5 cut(s) 830, 3024, 3607, 3782, 4030
SlaI CTCGAG 1 cut(s) 3402
SmiMI CAYNNNNRTG 7 cut(s) 816, 1117, 1156, 1669, 2142, 2153, 2699
SmlI CTYRAG 7 cut(s) 15, 1075, 2635, 3402, 3495, 3590, 4471
SmoI CTYRAG 7 cut(s) 15, 1075, 2635, 3402, 3495, 3590, 4471
SpeI ACTAGT 2 cut(s) 2612, 3034
SphI GCATGC 2 cut(s) 922, 2987
SseBI AGGCCT 1 cut(s) 1485
SsiI CCGC 2 cut(s) 186, 4199
SspI AATATT 5 cut(s) 376, 550, 2647, 2911, 3061
SstI GAGCTC 1 cut(s) 4459
StuI AGGCCT 1 cut(s) 1485
StyD4I CCNGG 8 cut(s) 65, 1138, 1231, 3026, 3128, 3325, 3630, 4025
StyI CCWWGG 4 cut(s) 2123, 3610, 3949, 4263
TaiI ACGT 1 cut(s) 4317
TaqI TCGA 4 cut(s) 2071, 3403, 3645, 4311
TatI WGTACW 6 cut(s) 1661, 1806, 1939, 2180, 3740, 4412
TscAI CASTG 9 cut(s) 280, 372, 643, 1294, 2727, 2844, 3883, 3910, 4362
TspGWI ACGGA 1 cut(s) 3978
TspRI CASTG 9 cut(s) 280, 372, 643, 1294, 2727, 2844, 3883, 3910, 4362
Van91I CCANNNNNTGG 2 cut(s) 878, 3443
VpaK11BI GGWCC 5 cut(s) 830, 3024, 3607, 3782, 4030
XapI RAATTY 5 cut(s) 113, 416, 838, 2726, 4173
XbaI TCTAGA 1 cut(s) 4121
XceI RCATGY 8 cut(s) 347, 922, 1254, 2145, 2408, 2987, 3359, 4304
XcmI CCANNNNNNNNNTGG 1 cut(s) 2127
XhoI CTCGAG 1 cut(s) 3402
XmaJI CCTAGG 1 cut(s) 3610
XmiI GTMKAC 5 cut(s) 1738, 1967, 2507, 2617, 4311
XmnI GAANNNNTTC 2 cut(s) 1878, 2894
ZrmI AGTACT 3 cut(s) 1808, 1941, 3742
Zsp2I ATGCAT 3 cut(s) 2143, 2327, 2706
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.