FvH4_4g32780

MLP-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000362
Physical Location & Seq
Forward (+)
31290809 .. 31293306
2498 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g32780.t1

Sequence Viewer

Length: 459 bp
ATGGCTCTGACTGGTAAGATAGAGACAGATGTAGATATCAAGGCTTCTGCTACAAAGTTCCACGAAATGTTCACCCACAAACCACACCACATTTCCAATGCCAGCTCCAGCAACATTCAAGGCTGTGAATTACATGAAGGTGACTGGGGAACAGTCGGTACTGTCATCTATTGGAATTACGTCCACGATGGGAAAGCTTGTGTTGCCAAGGAAATAGTTGAAGCCATAGACGCGGAAAAAAACTTGGTGACTTTCAGAGTCATTGAGGGAGACCTTATGGAGCATTACAAGAGCTTCTTGCTCACCATTCAAGCCAATCCGAAAGACGAGGGCTGCACTGTGCACTGGACTTTAGAGTATGAGAAGCATCACGGCGATATTACCGATCCGCATACGCTGCTCCAGTTTTGCGCCGAGGTTTCCAAAGACATCGATGCTCACCTTACTGCCGAAGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bet_v_1

Protein Analysis

153

Amino Acids

17.03

Weight (kDa)

5.22

Isoelectric Point (pI)

25.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bet_v_1 PF00407 2 - 150 2.7e-54 Pathogenesis-related protein Bet v 1 family
Polyketide_cyc2 PF10604 6 - 131 2.3e-07 Polyketide cyclase / dehydrase and lipid transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000362)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G23130 AT1G35260 AT1G35310 AT1G70830 AT1G70830 AT1G70830 AT1G70830 AT1G70830 AT1G70840 AT1G70850 AT1G70850 AT1G70850 AT1G70860 AT1G70860 AT1G70880 AT1G70890 AT5G28000 AT5G28010 AT5G28010
fragaria_vesca FvH4_4g32750 FvH4_4g32760 FvH4_4g32770 FvH4_4g32770 FvH4_4g32780
malus_domestica MD00G1063600.v1.1 MD13G1022700.v1.1 MD13G1022900.v1.1 MD13G1023000.v1.1 MD16G1024000.v1.1
prunus_persica Prupe.1G328500_v2.0.a1 Prupe.1G328600_v2.0.a1 Prupe.1G328800_v2.0.a1 Prupe.1G329000_v2.0.a1
pyrus_communis pycom16g02080
rosa_chinensis RchiOBHm_Chr1g0324501 RchiOBHm_Chr1g0324511 RchiOBHm_Chr4g0392511 RchiOBHm_Chr4g0392581 RchiOBHm_Chr4g0392611 RchiOBHm_Chr4g0441741 RchiOBHm_Chr4g0441751 RchiOBHm_Chr4g0441771
rosa_laevigata RLG00000006068 RLG00000006069 RLG00000006070 RLG00000009801 RLG00000030196 RLG00000030197 RLG00000034286
rosa_multiflora Rmu_co8369709.1_g000001 Rmu_co8434885.1_g000001 Rmu_sc0000353.1_g000003 Rmu_sc0000353.1_g000005 Rmu_sc0000498.1_g000002 Rmu_sc0000498.1_g000016 Rmu_sc0001866.1_g000004 Rmu_sc0003124.1_g000016 Rmu_sc0008279.1_g000010 Rmu_ssc0000486.1_g000033
rosa_roxburghii Rroxscaffold_159G00432880 Rroxscaffold_159G00432890 Rroxscaffold_4G00325460 Rroxscaffold_4G00325470 Rroxscaffold_5G00337770 Rroxscaffold_5G00382550 Rroxscaffold_5G00382570 Rroxscaffold_5G00382580
rosa_rugosa Rorug01G0046500 Rorug01G0046600 Rorug03G0339400 Rorug03G0339500 Rorug04G0335600 Rorug04G0335900.1 Rorug04G0336000 Rorug04G0336100
rosa_samantha Rh1AG062200 Rh1AG062300 Rh1BG052500 Rh1BG052600 Rh1CG064200 Rh1CG064400 Rh1DG068300 Rh1DG068400 Rh4AG043500 Rh4AG387200 Rh4AG387300 Rh4BG038400 Rh4BG038600 Rh4BG038800 Rh4BG400900 Rh4BG401000 Rh4CG046300 Rh4CG046600 Rh4CG416000 Rh4CG416100 Rh4DG040500 Rh4DG394300 Rh4DG394400 Rh5AG299400
rosa_wichuraiana Rw0G012190 Rw1G005260 Rw1G005280 Rw4G003410 Rw4G033400 Rw4G033410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 233
AciI CCGC 2 cut(s) 233, 389
AclWI GGATC 1 cut(s) 380
AfaI GTAC 1 cut(s) 160
AgsI TTSAA 3 cut(s) 119, 221, 311
AluBI AGCT 3 cut(s) 105, 197, 294
AluI AGCT 3 cut(s) 105, 197, 294
Alw21I GWGCWC 1 cut(s) 345
Alw26I GTCTC 2 cut(s) 17, 264
Alw44I GTGCAC 1 cut(s) 341
AlwI GGATC 1 cut(s) 380
ApaLI GTGCAC 1 cut(s) 341
ApeKI GCWGC 2 cut(s) 333, 397
AspLEI GCGC 1 cut(s) 413
AsuHPI GGTGA 5 cut(s) 64, 152, 259, 295, 431
BaeGI GKGCMC 1 cut(s) 345
Bbv12I GWGCWC 1 cut(s) 345
BbvI GCAGC 2 cut(s) 320, 384
BccI CCATC 1 cut(s) 182
BceAI ACGGC 1 cut(s) 388
BcoDI GTCTC 2 cut(s) 17, 264
BisI GCNGC 2 cut(s) 334, 398
BlsI GCNGC 2 cut(s) 335, 399
BmrI ACTGGG 1 cut(s) 154
BmsI GCATC 2 cut(s) 376, 424
BmuI ACTGGG 1 cut(s) 154
BpmI CTGGAG 2 cut(s) 91, 386
Bsa29I ATCGAT 1 cut(s) 432
BsaI GGTCTC 1 cut(s) 264
BsaJI CCNNGG 2 cut(s) 207, 414
Bse1I ACTGG 4 cut(s) 16, 149, 350, 403
BseCI ATCGAT 1 cut(s) 432
BseDI CCNNGG 2 cut(s) 207, 414
BseNI ACTGG 4 cut(s) 16, 149, 350, 403
BseSI GKGCMC 1 cut(s) 345
BseXI GCAGC 2 cut(s) 320, 384
BsgI GTGCAG 1 cut(s) 319
Bsh1236I CGCG 1 cut(s) 233
BshVI ATCGAT 1 cut(s) 432
BsiHKAI GWGCWC 1 cut(s) 345
BsmAI GTCTC 2 cut(s) 17, 264
Bso31I GGTCTC 1 cut(s) 264
Bsp1286I GDGCHC 1 cut(s) 345
Bsp143I GATC 1 cut(s) 385
BspACI CCGC 2 cut(s) 233, 389
BspDI ATCGAT 1 cut(s) 432
BspFNI CGCG 1 cut(s) 233
BspPI GGATC 1 cut(s) 380
BspTNI GGTCTC 1 cut(s) 264
BsrI ACTGG 4 cut(s) 16, 149, 350, 403
BssECI CCNNGG 2 cut(s) 207, 414
BssMI GATC 1 cut(s) 385
BssT1I CCWWGG 1 cut(s) 207
Bst4CI ACNGT 3 cut(s) 154, 163, 340
BstAPI GCANNNNNTGC 1 cut(s) 397
BstC8I GCNNGC 1 cut(s) 103
BstFNI CGCG 1 cut(s) 233
BstHHI GCGC 1 cut(s) 413
BstKTI GATC 1 cut(s) 388
BstMAI GTCTC 2 cut(s) 17, 264
BstMBI GATC 1 cut(s) 385
BstMWI GCNNNNNNNGC 3 cut(s) 203, 230, 397
BstSLI GKGCMC 1 cut(s) 345
BstUI CGCG 1 cut(s) 233
BstV1I GCAGC 2 cut(s) 320, 384
Bsu15I ATCGAT 1 cut(s) 432
BsuTUI ATCGAT 1 cut(s) 432
BtsIMutI CAGTG 2 cut(s) 336, 343
Cac8I GCNNGC 1 cut(s) 103
CfoI GCGC 1 cut(s) 413
ClaI ATCGAT 1 cut(s) 432
CseI GACGC 1 cut(s) 239
Csp6I GTAC 1 cut(s) 159
CviAII CATG 1 cut(s) 134
CviJI RGCY 9 cut(s) 5, 44, 105, 123, 197, 224, 294, 314, 333
CviKI_1 RGCY 9 cut(s) 5, 44, 105, 123, 197, 224, 294, 314, 333
CviQI GTAC 1 cut(s) 159
DpnI GATC 1 cut(s) 387
DpnII GATC 1 cut(s) 385
Eco130I CCWWGG 1 cut(s) 207
Eco31I GGTCTC 1 cut(s) 264
Eco32I GATATC 1 cut(s) 37
EcoRV GATATC 1 cut(s) 37
EcoT14I CCWWGG 1 cut(s) 207
ErhI CCWWGG 1 cut(s) 207
FaeI CATG 1 cut(s) 137
FaiI YATR 6 cut(s) 135, 227, 278, 360, 393, 457
FalI AAGNNNNNCTT 2 cut(s) 281, 313
FatI CATG 1 cut(s) 133
Fnu4HI GCNGC 2 cut(s) 334, 398
Fsp4HI GCNGC 2 cut(s) 334, 398
GlaI GCGC 1 cut(s) 412
GluI GCNGC 2 cut(s) 334, 398
GsuI CTGGAG 2 cut(s) 91, 386
HgaI GACGC 1 cut(s) 239
HhaI GCGC 1 cut(s) 413
Hin1II CATG 1 cut(s) 137
Hin6I GCGC 1 cut(s) 411
HinP1I GCGC 1 cut(s) 411
HindIII AAGCTT 1 cut(s) 195
HinfI GANTC 1 cut(s) 258
HphI GGTGA 5 cut(s) 64, 152, 259, 295, 431
Hpy166II GTNNAC 3 cut(s) 72, 184, 343
Hpy188I TCNGA 3 cut(s) 9, 257, 321
Hpy8I GTNNAC 3 cut(s) 72, 184, 343
HpyAV CCTTC 1 cut(s) 131
HpyCH4III ACNGT 3 cut(s) 154, 163, 340
HpyCH4IV ACGT 1 cut(s) 180
HpyCH4V TGCA 2 cut(s) 336, 343
HpyF10VI GCNNNNNNNGC 3 cut(s) 203, 230, 397
HpySE526I ACGT 1 cut(s) 180
Hsp92II CATG 1 cut(s) 137
HspAI GCGC 1 cut(s) 411
Kzo9I GATC 1 cut(s) 385
LmnI GCTCC 3 cut(s) 110, 280, 405
LpnPI CCDG 5 cut(s) 115, 121, 130, 331, 416
Lsp1109I GCAGC 2 cut(s) 320, 384
LweI GCATC 2 cut(s) 376, 424
MaeII ACGT 1 cut(s) 180
MaeIII GTNAC 2 cut(s) 140, 247
MalI GATC 1 cut(s) 387
MboI GATC 1 cut(s) 385
MhlI GDGCHC 1 cut(s) 345
MluCI AATT 2 cut(s) 128, 175
MlyI GAGTC 1 cut(s) 267
MnlI CCTC 3 cut(s) 259, 322, 409
MslI CAYNNNNRTG 1 cut(s) 138
MvnI CGCG 1 cut(s) 233
MwoI GCNNNNNNNGC 3 cut(s) 203, 230, 397
NdeII GATC 1 cut(s) 385
NlaIII CATG 1 cut(s) 137
NmeAIII GCCGAG 1 cut(s) 439
NmuCI GTSAC 2 cut(s) 140, 247
PkrI GCNGC 2 cut(s) 335, 399
PleI GAGTC 1 cut(s) 266
PpsI GAGTC 1 cut(s) 266
PsrI GAACNNNNNNTAC 2 cut(s) 142, 174
RsaI GTAC 1 cut(s) 160
RsaNI GTAC 1 cut(s) 159
RseI CAYNNNNRTG 1 cut(s) 138
SatI GCNGC 2 cut(s) 334, 398
Sau3AI GATC 1 cut(s) 385
SchI GAGTC 1 cut(s) 267
SduI GDGCHC 1 cut(s) 345
SetI ASST 8 cut(s) 107, 142, 183, 199, 276, 296, 420, 444
SfaNI GCATC 2 cut(s) 376, 424
SmiMI CAYNNNNRTG 1 cut(s) 138
Sse9I AATT 2 cut(s) 128, 175
SsiI CCGC 2 cut(s) 233, 389
StyI CCWWGG 1 cut(s) 207
TaaI ACNGT 3 cut(s) 154, 163, 340
TaiI ACGT 1 cut(s) 183
TaqI TCGA 1 cut(s) 432
TasI AATT 2 cut(s) 128, 175
TscAI CASTG 2 cut(s) 343, 350
TseFI GTSAC 2 cut(s) 140, 247
TseI GCWGC 2 cut(s) 333, 397
Tsp45I GTSAC 2 cut(s) 140, 247
TspDTI ATGAA 1 cut(s) 150
TspRI CASTG 2 cut(s) 343, 350
VneI GTGCAC 1 cut(s) 341
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.