MD13G1023000.v1.1

MLP-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0000362
Physical Location & Seq
Reverse (-)
1657247 .. 1658331
1085 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1023000.v1.1.491

Sequence Viewer

Length: 468 bp
ATGTCTAGCGATTGTGGTAAGGTGGAGGCCGATGTTGAAATCAAGGCTTCTGCTGCCAAGTTCCATGAAATGTTCACACACAAGCCACACCACATCTCCAATGTCAGCAATAGCAACATTCAGGGCTGTGATCTACATGAAGGTGATTGGGGAACCGTCGGATCTGTGGTCTACTGGAATTATTTCCATGATGGAAAAGCCAAAGTTGCTAAGGAGTTGATTGAAGCCATAGACGCTGAAAATAATCTGATCACTTTCAAAGTGATTGAAGGAGATCTTATGGAGCATTACAAGAGCTTCAAGATCACCATCCACGCAACTCCGAAAGGCGAGGGATGCAACGTGCACTGGACTATGGAGTATGAGAAGCACCATGACGACATTGAAGATCCACATACATTGCTCCAGTTTGCAGTTGAGGTCTCCAAAGATATTGATGCTCACCTTACAAGCACCGATGCAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bet_v_1

Protein Analysis

156

Amino Acids

17.45

Weight (kDa)

5.32

Isoelectric Point (pI)

26.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bet_v_1 PF00407 6 - 151 6.9e-56 Pathogenesis-related protein Bet v 1 family
Polyketide_cyc2 PF10604 7 - 131 1.6e-07 Polyketide cyclase / dehydrase and lipid transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000362)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G23130 AT1G35260 AT1G35310 AT1G70830 AT1G70830 AT1G70830 AT1G70830 AT1G70830 AT1G70840 AT1G70850 AT1G70850 AT1G70850 AT1G70860 AT1G70860 AT1G70880 AT1G70890 AT5G28000 AT5G28010 AT5G28010
fragaria_vesca FvH4_4g32750 FvH4_4g32760 FvH4_4g32770 FvH4_4g32770 FvH4_4g32780
malus_domestica MD00G1063600.v1.1 MD13G1022700.v1.1 MD13G1022900.v1.1 MD13G1023000.v1.1 MD16G1024000.v1.1
prunus_persica Prupe.1G328500_v2.0.a1 Prupe.1G328600_v2.0.a1 Prupe.1G328800_v2.0.a1 Prupe.1G329000_v2.0.a1
pyrus_communis pycom16g02080
rosa_chinensis RchiOBHm_Chr1g0324501 RchiOBHm_Chr1g0324511 RchiOBHm_Chr4g0392511 RchiOBHm_Chr4g0392581 RchiOBHm_Chr4g0392611 RchiOBHm_Chr4g0441741 RchiOBHm_Chr4g0441751 RchiOBHm_Chr4g0441771
rosa_laevigata RLG00000006068 RLG00000006069 RLG00000006070 RLG00000009801 RLG00000030196 RLG00000030197 RLG00000034286
rosa_multiflora Rmu_co8369709.1_g000001 Rmu_co8434885.1_g000001 Rmu_sc0000353.1_g000003 Rmu_sc0000353.1_g000005 Rmu_sc0000498.1_g000002 Rmu_sc0000498.1_g000016 Rmu_sc0001866.1_g000004 Rmu_sc0003124.1_g000016 Rmu_sc0008279.1_g000010 Rmu_ssc0000486.1_g000033
rosa_roxburghii Rroxscaffold_159G00432880 Rroxscaffold_159G00432890 Rroxscaffold_4G00325460 Rroxscaffold_4G00325470 Rroxscaffold_5G00337770 Rroxscaffold_5G00382550 Rroxscaffold_5G00382570 Rroxscaffold_5G00382580
rosa_rugosa Rorug01G0046500 Rorug01G0046600 Rorug03G0339400 Rorug03G0339500 Rorug04G0335600 Rorug04G0335900.1 Rorug04G0336000 Rorug04G0336100
rosa_samantha Rh1AG062200 Rh1AG062300 Rh1BG052500 Rh1BG052600 Rh1CG064200 Rh1CG064400 Rh1DG068300 Rh1DG068400 Rh4AG043500 Rh4AG387200 Rh4AG387300 Rh4BG038400 Rh4BG038600 Rh4BG038800 Rh4BG400900 Rh4BG401000 Rh4CG046300 Rh4CG046600 Rh4CG416000 Rh4CG416100 Rh4DG040500 Rh4DG394300 Rh4DG394400 Rh5AG299400
rosa_wichuraiana Rw0G012190 Rw1G005260 Rw1G005280 Rw4G003410 Rw4G033400 Rw4G033410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 171
AclWI GGATC 2 cut(s) 169, 383
AgsI TTSAA 6 cut(s) 38, 224, 259, 269, 301, 386
AluBI AGCT 1 cut(s) 297
AluI AGCT 1 cut(s) 297
Alw21I GWGCWC 1 cut(s) 348
Alw26I GTCTC 1 cut(s) 427
Alw44I GTGCAC 1 cut(s) 344
AlwI GGATC 2 cut(s) 169, 383
AoxI GGCC 1 cut(s) 27
ApaLI GTGCAC 1 cut(s) 344
ApeKI GCWGC 1 cut(s) 53
Asp700I GAANNNNTTC 1 cut(s) 182
AsuHPI GGTGA 3 cut(s) 155, 298, 434
BaeGI GKGCMC 1 cut(s) 348
Bbv12I GWGCWC 1 cut(s) 348
BbvI GCAGC 1 cut(s) 40
BccI CCATC 2 cut(s) 185, 317
BclI TGATCA 1 cut(s) 249
BcoDI GTCTC 1 cut(s) 427
BfaI CTAG 1 cut(s) 6
BglII AGATCT 1 cut(s) 274
BisI GCNGC 1 cut(s) 54
BlsI GCNGC 1 cut(s) 55
BmiI GGNNCC 1 cut(s) 154
BmsI GCATC 3 cut(s) 326, 427, 448
BpmI CTGGAG 1 cut(s) 389
Bpu10I CCTNAGC 1 cut(s) 210
BsaBI GATNNNNATC 1 cut(s) 308
BsaI GGTCTC 1 cut(s) 427
BsaXI ACNNNNNCTCC 2 cut(s) 80, 110
Bse1I ACTGG 3 cut(s) 179, 353, 406
Bse3DI GCAATG 1 cut(s) 398
Bse8I GATNNNNATC 1 cut(s) 308
BseGI GGATG 2 cut(s) 309, 341
BseJI GATNNNNATC 1 cut(s) 308
BseMI GCAATG 1 cut(s) 398
BseNI ACTGG 3 cut(s) 179, 353, 406
BseSI GKGCMC 1 cut(s) 348
BseXI GCAGC 1 cut(s) 40
BshFI GGCC 1 cut(s) 29
BsiHKAI GWGCWC 1 cut(s) 348
BsmAI GTCTC 1 cut(s) 427
BsnI GGCC 1 cut(s) 29
Bso31I GGTCTC 1 cut(s) 427
Bsp1286I GDGCHC 1 cut(s) 348
Bsp143I GATC 6 cut(s) 130, 161, 249, 274, 303, 388
BspANI GGCC 1 cut(s) 29
BspLI GGNNCC 1 cut(s) 154
BspPI GGATC 2 cut(s) 169, 383
BspTNI GGTCTC 1 cut(s) 427
BsrDI GCAATG 1 cut(s) 398
BsrI ACTGG 3 cut(s) 179, 353, 406
BssMI GATC 6 cut(s) 130, 161, 249, 274, 303, 388
Bst4CI ACNGT 1 cut(s) 157
BstDEI CTNAG 1 cut(s) 210
BstF5I GGATG 2 cut(s) 309, 341
BstKTI GATC 6 cut(s) 133, 164, 252, 277, 306, 391
BstMAI GTCTC 1 cut(s) 427
BstMBI GATC 6 cut(s) 130, 161, 249, 274, 303, 388
BstMWI GCNNNNNNNGC 4 cut(s) 53, 206, 233, 336
BstSLI GKGCMC 1 cut(s) 348
BstV1I GCAGC 1 cut(s) 40
BstX2I RGATCY 3 cut(s) 161, 274, 388
BstYI RGATCY 3 cut(s) 161, 274, 388
BsuRI GGCC 1 cut(s) 29
BtsCI GGATG 2 cut(s) 309, 341
BtsIMutI CAGTG 1 cut(s) 346
CseI GACGC 1 cut(s) 242
CspCI CAANNNNNGTGG 2 cut(s) 381, 416
CviAII CATG 4 cut(s) 65, 137, 188, 374
CviJI RGCY 7 cut(s) 29, 47, 85, 126, 200, 227, 297
CviKI_1 RGCY 7 cut(s) 29, 47, 85, 126, 200, 227, 297
DdeI CTNAG 1 cut(s) 210
DpnI GATC 6 cut(s) 132, 163, 251, 276, 305, 390
DpnII GATC 6 cut(s) 130, 161, 249, 274, 303, 388
Eco31I GGTCTC 1 cut(s) 427
FaeI CATG 4 cut(s) 68, 140, 191, 377
FaiI YATR 9 cut(s) 66, 138, 189, 230, 281, 356, 363, 375, 396
FalI AAGNNNNNCTT 2 cut(s) 261, 293
FatI CATG 4 cut(s) 64, 136, 187, 373
FbaI TGATCA 1 cut(s) 249
FblI GTMKAC 1 cut(s) 171
Fnu4HI GCNGC 1 cut(s) 54
FokI GGATG 2 cut(s) 296, 348
Fsp4HI GCNGC 1 cut(s) 54
FspBI CTAG 1 cut(s) 6
GluI GCNGC 1 cut(s) 54
GsuI CTGGAG 1 cut(s) 389
HaeIII GGCC 1 cut(s) 29
HgaI GACGC 1 cut(s) 242
Hin1II CATG 4 cut(s) 68, 140, 191, 377
HphI GGTGA 3 cut(s) 155, 298, 434
Hpy166II GTNNAC 3 cut(s) 75, 172, 346
Hpy188I TCNGA 3 cut(s) 161, 249, 324
Hpy188III TCNNGA 1 cut(s) 301
Hpy8I GTNNAC 3 cut(s) 75, 172, 346
Hpy99I CGWCG 1 cut(s) 161
HpyAV CCTTC 2 cut(s) 134, 263
HpyCH4III ACNGT 1 cut(s) 157
HpyCH4IV ACGT 1 cut(s) 342
HpyCH4V TGCA 4 cut(s) 339, 346, 413, 461
HpyF10VI GCNNNNNNNGC 4 cut(s) 53, 206, 233, 336
HpyF3I CTNAG 1 cut(s) 210
HpySE526I ACGT 1 cut(s) 342
Hsp92II CATG 4 cut(s) 68, 140, 191, 377
Ksp22I TGATCA 1 cut(s) 249
Kzo9I GATC 6 cut(s) 130, 161, 249, 274, 303, 388
LmnI GCTCC 2 cut(s) 283, 408
LpnPI CCDG 4 cut(s) 107, 160, 334, 419
Lsp1109I GCAGC 1 cut(s) 40
LweI GCATC 3 cut(s) 326, 427, 448
MaeI CTAG 1 cut(s) 6
MaeII ACGT 1 cut(s) 342
MalI GATC 6 cut(s) 132, 163, 251, 276, 305, 390
MboI GATC 6 cut(s) 130, 161, 249, 274, 303, 388
MboII GAAGA 1 cut(s) 398
MflI RGATCY 3 cut(s) 161, 274, 388
MhlI GDGCHC 1 cut(s) 348
MluCI AATT 1 cut(s) 178
MmeI TCCRAC 1 cut(s) 139
MnlI CCTC 3 cut(s) 19, 325, 412
MroXI GAANNNNTTC 1 cut(s) 182
MslI CAYNNNNRTG 1 cut(s) 141
MwoI GCNNNNNNNGC 4 cut(s) 53, 206, 233, 336
NdeII GATC 6 cut(s) 130, 161, 249, 274, 303, 388
NlaIII CATG 4 cut(s) 68, 140, 191, 377
NlaIV GGNNCC 1 cut(s) 154
PdmI GAANNNNTTC 1 cut(s) 182
PkrI GCNGC 1 cut(s) 55
PspN4I GGNNCC 1 cut(s) 154
PsuI RGATCY 3 cut(s) 161, 274, 388
RseI CAYNNNNRTG 1 cut(s) 141
SatI GCNGC 1 cut(s) 54
Sau3AI GATC 6 cut(s) 130, 161, 249, 274, 303, 388
SduI GDGCHC 1 cut(s) 348
SetI ASST 6 cut(s) 24, 145, 299, 345, 423, 447
SfaNI GCATC 3 cut(s) 326, 427, 448
SmiMI CAYNNNNRTG 1 cut(s) 141
Sse9I AATT 1 cut(s) 178
SspMI CTAG 1 cut(s) 6
TaaI ACNGT 1 cut(s) 157
TaiI ACGT 1 cut(s) 345
TasI AATT 1 cut(s) 178
TscAI CASTG 1 cut(s) 353
TseI GCWGC 1 cut(s) 53
TspDTI ATGAA 2 cut(s) 81, 153
TspRI CASTG 1 cut(s) 353
VneI GTGCAC 1 cut(s) 344
XmiI GTMKAC 1 cut(s) 171
XmnI GAANNNNTTC 1 cut(s) 182
XspI CTAG 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.