FvH4_5g16511

Domain of unknown function (DUF4283)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001839
Physical Location & Seq
Forward (+)
9385966 .. 9389158
3193 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g16511.t1

Sequence Viewer

Length: 1968 bp
ATGGTGGAGACATTAAGACCATTACTACCCGGAAAGGCTTATGTAAAAGGTGAAGTACTTTTGTTTAGAAATCTTGATTTAGAAAAGGCGAGTTTGGTTGTGTTCCATGAAATTGCAAAGCATTTGGGTTATAAGCGAAGCTCTAGATTCTGGTACAAGCAACCTGATGGAGAATTTTCAGAAGTTCGAGATGACGTTCGTCTAAGTGAAATTGTTAAGAATGAACTATCAGTTGACCGTGAGTTGAATATTTATCTCGTTGATGGAGAAAGAGAAGAAACACAAGTGGAACAAGCTCCCGCCATAATGTTTTGGAACTGTCATGGTCTCAATGACGACAAAGTATATGAACTGCAAGCTCTTAGTCTTATTTACCAACCAAATATTCTTTTCGTCATCGAGACCCGGTTGCCATCCACGTCTTCCAGTAATATCTGTTGCACGCTAGGTTTCAAAGAGTTTAGGGATTCTCCGGCAACCAAACATATGGAAGGTTTAAAAGGCTGCGTGGGGTTAGCCATGTTTTGGACAAAAGATGTAAACCTTGAGTTTCACGATGGTTCAGATAATTATCTGGTGTTTATGGTTATAGTATCTAATTCTGAAAGGCACAGGTTGATAGGCTTCTACGGCGACCCAAAAGATAAGAATCTCTCATGGGAGTTGCTCGTGCAATTATCAAGTAAATCGGCTGAAGACCGATGGGTATCGACTGAAGACCGATGGGTAGTTGGTGGAGATTTTAATGAAATCTTGAAACATAAAGAGAAAAGTGGCGGAGACCTTGCCATGACCCCTCCTATTGACTGGAAGGAAGGCCTTATTAAGGTGGCGCAAAAACTACAAGAAGCTATTATGAGTTTGGAACATCTAGACGTTACAACGGTTCCTCGTTTGCAAAGGAAGCTGGACAAAATGCCGGATTTATATTATGAAGCTGTCAAGCTCGTTGCTCCTCATCTCATTCAACTAGAGGATATTGCAGTGCGCGAAAATGATAAATTTCCATATTGTGGAAGAGACGAGAAAATTCCGCTTAATCTTTCAGAATATCTAGCGCAAACATGGTTGCTCATAAAGTTCATTCCAGCAGCAGATTGTGAAGAACCTCAACATCAGATTGTGAAGAAAATAATAGAGAAGCAAGTGAAAAATTTAATAATATGGTTTGAAAAGTTAAGTACAAGATATGGTACTGGTGATGATATAAGTACGAAATTAAAGGAGAAGGCATCAATGATTAGTCGTGCTATCTTCGTAAGTAAGAAATTAAAGGAGAAGGCATCAATGATTGGTCGTGCTATCTACGTGTACGACATCGTCAGCTCTCTACTTCAGAAAGAGAGGATCAGACATGAAGGCACAATAAAAGAACTTGAGAAAGAAGTTTGTCATGTTTGCGAGTATTACTATGGAGAAGCATCGATTGCGGTTAGTTCAAGAGAAATGATATGTGAGCCTCTTCAGTTTTTACTAGAGAAGTTTCAATGGCGCCATCCCGATACTTCTCTTAAATACAGGATTACTGGTAATTCTGAAGAGCAATTTGCTGAACTTACTGAACAAATACTTGAAGTAGAGACAGAAGCAGCCGTGATAAACAATCCATGGATAAATTCGATTATAGAGGGTATTGAAAAAGAGAAGCCTGAAAATCTCGTTGGAACGGGTACAATTCAACGCCAGCTTGAGGTGTTAATAAAAGATATTGGTTGCATTGCATGTTCAGAAAATGGTAAAATTGCTTTATCCAAAGATGATGAAAACGGCGATTCAATAATTCTGCGACTTCAGATGGCAGGGAGTGAAGCTTTGGAAACGCTCTCGAGAAGTAAAAAGACTACGACTTATGAAGCGTACATGCGCAAGCTTATAGAGGCATACAACAGAAGAAGTGATCAGAGCAAAAAGAAGCGGGAGGTGGTGGGAGATGGTGGGAAACAAGGAGAGAAAAAGGCTAGAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 zf-CCHC_4

Protein Analysis

656

Amino Acids

75.15

Weight (kDa)

5.75

Isoelectric Point (pI)

40.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1-like PF26130 13 - 86 7.7e-07 PB1-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 132
Acc16I TGCGCA 1 cut(s) 1865
AccB1I GGYRCC 1 cut(s) 1491
AccB7I CCANNNNNTGG 2 cut(s) 525, 1013
AccII CGCG 1 cut(s) 990
AciI CCGC 5 cut(s) 300, 777, 1034, 1430, 1915
AclWI GGATC 1 cut(s) 1355
AcsI RAATTY 5 cut(s) 173, 1001, 1029, 1153, 1615
AcuI CTGAAG 6 cut(s) 714, 735, 1319, 1448, 1557, 1775
AcyI GRCGYC 1 cut(s) 1492
AfaI GTAC 8 cut(s) 57, 155, 1183, 1195, 1213, 1313, 1672, 1859
AfiI CCNNNNNNNGG 4 cut(s) 525, 826, 1013, 1690
AflIII ACRYGT 1 cut(s) 1308
AjiI CACGTC 1 cut(s) 420
AjuI GAANNNNNNNTTGG 4 cut(s) 369, 401, 1644, 1676
Alw26I GTCTC 6 cut(s) 2, 332, 395, 774, 1014, 1574
AlwI GGATC 1 cut(s) 1355
Ama87I CYCGRG 1 cut(s) 1825
AoxI GGCC 1 cut(s) 817
ApeKI GCWGC 3 cut(s) 504, 1091, 1589
ApoI RAATTY 5 cut(s) 173, 1001, 1029, 1153, 1615
AspLEI GCGC 5 cut(s) 835, 990, 1060, 1494, 1866
AsuC2I CCSGG 2 cut(s) 30, 406
AsuHPI GGTGA 2 cut(s) 62, 1211
AvaI CYCGRG 1 cut(s) 1825
BanI GGYRCC 1 cut(s) 1491
BauI CACGAG 1 cut(s) 668
BbsI GAAGAC 3 cut(s) 414, 702, 723
BbvI GCAGC 3 cut(s) 491, 1103, 1601
BccI CCATC 9 cut(s) 161, 257, 421, 551, 696, 717, 1503, 1789, 1925
BceAI ACGGC 3 cut(s) 646, 1577, 1783
BclI TGATCA 1 cut(s) 1897
BcnI CCSGG 2 cut(s) 30, 406
BcoDI GTCTC 6 cut(s) 2, 332, 395, 774, 1014, 1574
BfaI CTAG 7 cut(s) 144, 446, 872, 971, 1055, 1475, 1959
BfoI RGCGCY 1 cut(s) 1495
BisI GCNGC 3 cut(s) 505, 1092, 1590
BlsI GCNGC 3 cut(s) 506, 1093, 1591
BmcAI AGTACT 1 cut(s) 57
Bme1390I CCNGG 2 cut(s) 30, 406
BmeT110I CYCGRG 1 cut(s) 1825
BmgBI CACGTC 1 cut(s) 420
BmiI GGNNCC 2 cut(s) 888, 1493
BmrFI CCNGG 2 cut(s) 30, 406
BmsI GCATC 3 cut(s) 1241, 1292, 1430
BoxI GACNNNNGTC 1 cut(s) 198
BpiI GAAGAC 3 cut(s) 414, 702, 723
BpuEI CTTGAG 3 cut(s) 566, 1397, 1709
BpuMI CCSGG 2 cut(s) 30, 406
Bsa29I ATCGAT 1 cut(s) 1424
BsaAI YACGTR 1 cut(s) 1309
BsaBI GATNNNNATC 3 cut(s) 570, 648, 706
BsaHI GRCGYC 1 cut(s) 1492
BsaI GGTCTC 3 cut(s) 332, 395, 774
BsaJI CCNNGG 1 cut(s) 1607
Bsc4I CCNNNNNNNGG 4 cut(s) 525, 826, 1013, 1690
Bse1I ACTGG 4 cut(s) 426, 812, 1201, 1531
Bse3DI GCAATG 1 cut(s) 1716
Bse8I GATNNNNATC 3 cut(s) 570, 648, 706
BseCI ATCGAT 1 cut(s) 1424
BseDI CCNNGG 1 cut(s) 1607
BseGI GGATG 2 cut(s) 413, 1495
BseJI GATNNNNATC 3 cut(s) 570, 648, 706
BseLI CCNNNNNNNGG 4 cut(s) 525, 826, 1013, 1690
BseMI GCAATG 1 cut(s) 1716
BseNI ACTGG 4 cut(s) 426, 812, 1201, 1531
BseRI GAGGAG 1 cut(s) 945
BseXI GCAGC 3 cut(s) 491, 1103, 1601
Bsh1236I CGCG 1 cut(s) 990
BshFI GGCC 1 cut(s) 819
BshNI GGYRCC 1 cut(s) 1491
BshVI ATCGAT 1 cut(s) 1424
BsiHKCI CYCGRG 1 cut(s) 1825
BsiSI CCGG 4 cut(s) 30, 406, 473, 920
BslI CCNNNNNNNGG 4 cut(s) 525, 826, 1013, 1690
BsmAI GTCTC 6 cut(s) 2, 332, 395, 774, 1014, 1574
BsmBI CGTCTC 1 cut(s) 1014
BsnI GGCC 1 cut(s) 819
Bso31I GGTCTC 3 cut(s) 332, 395, 774
BsoBI CYCGRG 1 cut(s) 1825
Bsp143I GATC 2 cut(s) 1347, 1897
Bsp19I CCATGG 1 cut(s) 1607
BspACI CCGC 5 cut(s) 300, 777, 1034, 1430, 1915
BspANI GGCC 1 cut(s) 819
BspDI ATCGAT 1 cut(s) 1424
BspFNI CGCG 1 cut(s) 990
BspLI GGNNCC 2 cut(s) 888, 1493
BspPI GGATC 1 cut(s) 1355
BspQI GCTCTTC 1 cut(s) 1533
BspT107I GGYRCC 1 cut(s) 1491
BspTNI GGTCTC 3 cut(s) 332, 395, 774
BsrDI GCAATG 1 cut(s) 1716
BsrI ACTGG 4 cut(s) 426, 812, 1201, 1531
BssECI CCNNGG 1 cut(s) 1607
BssMI GATC 2 cut(s) 1347, 1897
BssNI GRCGYC 1 cut(s) 1492
BssSI CACGAG 1 cut(s) 668
BssT1I CCWWGG 1 cut(s) 1607
Bst2BI CACGAG 1 cut(s) 668
Bst4CI ACNGT 3 cut(s) 239, 320, 886
Bst6I CTCTTC 3 cut(s) 1012, 1467, 1533
BstACI GRCGYC 1 cut(s) 1492
BstAPI GCANNNNNTGC 1 cut(s) 1427
BstBAI YACGTR 1 cut(s) 1309
BstC8I GCNNGC 4 cut(s) 357, 443, 1685, 1868
BstDEI CTNAG 2 cut(s) 203, 362
BstDSI CCRYGG 1 cut(s) 1607
BstENI CCTNNNNNAGG 1 cut(s) 824
BstF5I GGATG 2 cut(s) 413, 1495
BstFNI CGCG 1 cut(s) 990
BstH2I RGCGCY 1 cut(s) 1495
BstHHI GCGC 5 cut(s) 835, 990, 1060, 1494, 1866
BstKTI GATC 2 cut(s) 1350, 1900
BstMAI GTCTC 6 cut(s) 2, 332, 395, 774, 1014, 1574
BstMBI GATC 2 cut(s) 1347, 1897
BstMWI GCNNNNNNNGC 3 cut(s) 630, 904, 1427
BstNSI RCATGY 2 cut(s) 1725, 1864
BstPAI GACNNNNGTC 1 cut(s) 198
BstSCI CCNGG 2 cut(s) 28, 404
BstUI CGCG 1 cut(s) 990
BstV1I GCAGC 3 cut(s) 491, 1103, 1601
BstV2I GAAGAC 3 cut(s) 414, 702, 723
BstXI CCANNNNNNTGG 1 cut(s) 487
Bsu15I ATCGAT 1 cut(s) 1424
BsuRI GGCC 1 cut(s) 819
BsuTUI ATCGAT 1 cut(s) 1424
BtgI CCRYGG 1 cut(s) 1607
BtrI CACGTC 1 cut(s) 420
BtsCI GGATG 2 cut(s) 413, 1495
BtsI GCAGTG 1 cut(s) 990
BtsIMutI CAGTG 1 cut(s) 990
Cac8I GCNNGC 4 cut(s) 357, 443, 1685, 1868
CfoI GCGC 5 cut(s) 835, 990, 1060, 1494, 1866
ClaI ATCGAT 1 cut(s) 1424
Csp6I GTAC 8 cut(s) 56, 154, 1182, 1194, 1212, 1312, 1671, 1858
CviQI GTAC 8 cut(s) 56, 154, 1182, 1194, 1212, 1312, 1671, 1858
DdeI CTNAG 2 cut(s) 203, 362
DinI GGCGCC 1 cut(s) 1493
DpnI GATC 2 cut(s) 1349, 1899
DpnII GATC 2 cut(s) 1347, 1897
DraI TTTAAA 1 cut(s) 498
Eam1104I CTCTTC 3 cut(s) 1012, 1467, 1533
EarI CTCTTC 3 cut(s) 1012, 1467, 1533
EciI GGCGGA 1 cut(s) 792
Eco130I CCWWGG 1 cut(s) 1607
Eco147I AGGCCT 1 cut(s) 819
Eco31I GGTCTC 3 cut(s) 332, 395, 774
Eco57I CTGAAG 6 cut(s) 714, 735, 1319, 1448, 1557, 1775
Eco88I CYCGRG 1 cut(s) 1825
EcoNI CCTNNNNNAGG 1 cut(s) 824
EcoT14I CCWWGG 1 cut(s) 1607
EgeI GGCGCC 1 cut(s) 1493
EheI GGCGCC 1 cut(s) 1493
ErhI CCWWGG 1 cut(s) 1607
Esp3I CGTCTC 1 cut(s) 1014
FauI CCCGC 2 cut(s) 307, 1908
FauNDI CATATG 1 cut(s) 486
FbaI TGATCA 1 cut(s) 1897
Fnu4HI GCNGC 3 cut(s) 505, 1092, 1590
FokI GGATG 2 cut(s) 400, 1482
Fsp4HI GCNGC 3 cut(s) 505, 1092, 1590
FspBI CTAG 7 cut(s) 144, 446, 872, 971, 1055, 1475, 1959
FspI TGCGCA 1 cut(s) 1865
GlaI GCGC 5 cut(s) 834, 989, 1059, 1493, 1865
GluI GCNGC 3 cut(s) 505, 1092, 1590
HaeII RGCGCY 1 cut(s) 1495
HaeIII GGCC 1 cut(s) 819
HapII CCGG 4 cut(s) 30, 406, 473, 920
HhaI GCGC 5 cut(s) 835, 990, 1060, 1494, 1866
Hin1I GRCGYC 1 cut(s) 1492
Hin6I GCGC 5 cut(s) 833, 988, 1058, 1492, 1864
HinP1I GCGC 5 cut(s) 833, 988, 1058, 1492, 1864
HincII GTYRAC 1 cut(s) 235
HindII GTYRAC 1 cut(s) 235
HindIII AAGCTT 2 cut(s) 1809, 1868
HinfI GANTC 4 cut(s) 147, 467, 649, 1772
HpaII CCGG 4 cut(s) 30, 406, 473, 920
HphI GGTGA 2 cut(s) 62, 1211
Hpy166II GTNNAC 3 cut(s) 235, 541, 1312
Hpy8I GTNNAC 3 cut(s) 235, 541, 1312
HpyAV CCTTC 6 cut(s) 485, 805, 809, 1222, 1273, 1352
HpyCH4III ACNGT 3 cut(s) 239, 320, 886
HpyCH4IV ACGT 4 cut(s) 195, 419, 876, 1308
HpyCH4V TGCA 8 cut(s) 116, 355, 441, 673, 898, 983, 1716, 1721
HpyF10VI GCNNNNNNNGC 3 cut(s) 630, 904, 1427
HpyF3I CTNAG 2 cut(s) 203, 362
HpySE526I ACGT 4 cut(s) 195, 419, 876, 1308
Hsp92I GRCGYC 1 cut(s) 1492
HspAI GCGC 5 cut(s) 833, 988, 1058, 1492, 1864
KasI GGCGCC 1 cut(s) 1491
Ksp22I TGATCA 1 cut(s) 1897
Kzo9I GATC 2 cut(s) 1347, 1897
LguI GCTCTTC 1 cut(s) 1533
LmnI GCTCC 2 cut(s) 301, 958
Lsp1109I GCAGC 3 cut(s) 491, 1103, 1601
LweI GCATC 3 cut(s) 1241, 1292, 1430
MaeI CTAG 7 cut(s) 144, 446, 872, 971, 1055, 1475, 1959
MaeII ACGT 4 cut(s) 195, 419, 876, 1308
MaeIII GTNAC 1 cut(s) 877
MalI GATC 2 cut(s) 1349, 1899
MboI GATC 2 cut(s) 1347, 1897
Mly113I GGCGCC 1 cut(s) 1492
MmeI TCCRAC 1 cut(s) 1642
MspI CCGG 4 cut(s) 30, 406, 473, 920
MspR9I CCNGG 2 cut(s) 30, 406
MvnI CGCG 1 cut(s) 990
MwoI GCNNNNNNNGC 3 cut(s) 630, 904, 1427
NarI GGCGCC 1 cut(s) 1492
NciI CCSGG 2 cut(s) 30, 406
NcoI CCATGG 1 cut(s) 1607
NdeI CATATG 1 cut(s) 486
NdeII GATC 2 cut(s) 1347, 1897
NlaIV GGNNCC 2 cut(s) 888, 1493
NsbI TGCGCA 1 cut(s) 1865
NspI RCATGY 2 cut(s) 1725, 1864
PaeR7I CTCGAG 1 cut(s) 1825
PceI AGGCCT 1 cut(s) 819
PciSI GCTCTTC 1 cut(s) 1533
PfeI GAWTC 4 cut(s) 147, 467, 649, 1772
PflFI GACNNNGTC 1 cut(s) 1319
PflMI CCANNNNNTGG 2 cut(s) 525, 1013
PkrI GCNGC 3 cut(s) 506, 1093, 1591
PluTI GGCGCC 1 cut(s) 1495
Ppu21I YACGTR 1 cut(s) 1309
PshAI GACNNNNGTC 1 cut(s) 198
PsiI TTATAA 1 cut(s) 132
PspN4I GGNNCC 2 cut(s) 888, 1493
PsyI GACNNNGTC 1 cut(s) 1319
RsaI GTAC 8 cut(s) 57, 155, 1183, 1195, 1213, 1313, 1672, 1859
RsaNI GTAC 8 cut(s) 56, 154, 1182, 1194, 1212, 1312, 1671, 1858
SapI GCTCTTC 1 cut(s) 1533
SatI GCNGC 3 cut(s) 505, 1092, 1590
Sau3AI GATC 2 cut(s) 1347, 1897
ScaI AGTACT 1 cut(s) 57
ScrFI CCNGG 2 cut(s) 30, 406
SfaNI GCATC 3 cut(s) 1241, 1292, 1430
SfoI GGCGCC 1 cut(s) 1493
Sfr274I CTCGAG 1 cut(s) 1825
SlaI CTCGAG 1 cut(s) 1825
SmlI CTYRAG 4 cut(s) 545, 1376, 1688, 1825
SmoI CTYRAG 4 cut(s) 545, 1376, 1688, 1825
SseBI AGGCCT 1 cut(s) 819
SsiI CCGC 5 cut(s) 300, 777, 1034, 1430, 1915
SspDI GGCGCC 1 cut(s) 1491
SspI AATATT 2 cut(s) 250, 385
SspMI CTAG 7 cut(s) 144, 446, 872, 971, 1055, 1475, 1959
StuI AGGCCT 1 cut(s) 819
StyD4I CCNGG 2 cut(s) 28, 404
StyI CCWWGG 1 cut(s) 1607
TaaI ACNGT 3 cut(s) 239, 320, 886
TaiI ACGT 4 cut(s) 198, 422, 879, 1311
TaqI TCGA 6 cut(s) 187, 399, 710, 1424, 1619, 1826
TaqII GACCGA 2 cut(s) 714, 735
TatI WGTACW 2 cut(s) 55, 1181
TfiI GAWTC 4 cut(s) 147, 467, 649, 1772
TscAI CASTG 1 cut(s) 990
TseI GCWGC 3 cut(s) 504, 1091, 1589
TspDTI ATGAA 9 cut(s) 123, 237, 363, 762, 948, 1072, 1371, 1776, 1866
TspRI CASTG 1 cut(s) 990
Tth111I GACNNNGTC 1 cut(s) 1319
Van91I CCANNNNNTGG 2 cut(s) 525, 1013
XagI CCTNNNNNAGG 1 cut(s) 824
XapI RAATTY 5 cut(s) 173, 1001, 1029, 1153, 1615
XbaI TCTAGA 2 cut(s) 143, 871
XceI RCATGY 2 cut(s) 1725, 1864
XhoI CTCGAG 1 cut(s) 1825
XspI CTAG 7 cut(s) 144, 446, 872, 971, 1055, 1475, 1959
ZrmI AGTACT 1 cut(s) 57
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.