Rroxscaffold_3G00274960

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0001839
Physical Location & Seq
Reverse (-)
66559529 .. 66562496
2968 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00274960.1

Sequence Viewer

Length: 1275 bp
ATGATCAAGGAACTGCCAGTTACTCGAGAGTTGGAAATATATTTCGATGGAGTTAGAAAAGAAGAAAAACCGGAAATGTATCCTACTATTTTGTTCTGGAACTGCTGTGGACTTGATGACTCAGCCATATATATGCTAAAAGTTCTTATACAGATCTATGACCCTTCAATTATTTTCCTTATAGAGACTCGCTTGTCGTCTGAGGTTATGAAAGAAGTCTCTTTCTCACTAGGGTCTCATAATGTTTGGGATTCTCCTACAAGCAGAGGGGCAGCCGGTAAAGATAACTGTGTTGGCTTATCCATGTTATTGAGAACAAATGTAAATGTCGACTTTTTAAATAAGACATGCAATTATCTTGATTTCGTGATTAACGTAAGTCCTACTGGTCAGAAGTGGAGGTTGACAGGATTCTATGGGGACCCAAAATTGAAGTACCGCTCATGGGAGTGGCTCCGAAGCTTATCAAAAATTGAGAAGGATCTCCCGTGGATAATTTTTGGTGACTTTAATGAAATTTTATATCAGCATGAGAAAAATGGTGGACCGGAAAAGAGCAAATCAAAGATAAAGGCATTCAAGAATGTCGTCAAAGAATGTGGTCTTTGTGATGACATGCCTTTTGAGGGTGGTAAATTTACTTGGAGGAGAGATAACATTAAGCAGAGACTTGATAGAGTATTGGTCTCAGCGAAATGGAAAACAATATTTTCTTCTCCTAGGGTATTGCATCTGAAGGATGGAGATATATCAGATCACATTCCGATAGTGATACAAACTGATGGTCGAAAACAAATAGTAGAAAATTCACGAACTCTATATGATGAGATGGCTGATGTGTTTTTATTTGCTGAACAGTTGGCAGAGTTGTATCGTAATCCGAAAGTTAGGCTTATGACGGTCTCCAATATTGTAAACGAGATACAGCTGGAACGCTCGGATTTCTATTTCGAAGCAGTAAAGACGATTGCGCCTGAAGTTATTCATGCAATGTATATTGCAGTACATGATAAGAAAACTGATACGGTTAAATATGAACGTCTGGTTACTTTGAGGACTTGTTATAGCCTTGATCTCATATTATGGAATTGCATGGTCCTCCCCAAGTCAAGTCAAGATGACGACCTTGAAATTCCACCAGAATGTCCAAATGCTTCTTGTAAAAGAATAATCACAAATGAGGTTAATCTTTCGGAGTATCTTGCGCAAACATGGAAACTTGTATGGTTGATTCCAATTGGTGGTAAAATAACCCGGAAAGAGATTATTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

424

Amino Acids

49.16

Weight (kDa)

6.37

Isoelectric Point (pI)

44.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 32 - 253 1.4e-07 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 193
Acc16I TGCGCA 1 cut(s) 1206
AccB7I CCANNNNNTGG 1 cut(s) 1241
AccBSI CCGCTC 1 cut(s) 441
AccI GTMKAC 1 cut(s) 330
AciI CCGC 1 cut(s) 439
AclWI GGATC 1 cut(s) 489
AcsI RAATTY 4 cut(s) 516, 635, 805, 1131
AcuI CTGAAG 2 cut(s) 755, 996
AfaI GTAC 2 cut(s) 437, 1005
AfiI CCNNNNNNNGG 3 cut(s) 445, 626, 1241
AgsI TTSAA 4 cut(s) 168, 433, 580, 1130
AluBI AGCT 2 cut(s) 462, 928
AluI AGCT 2 cut(s) 462, 928
Alw26I GTCTC 6 cut(s) 179, 223, 240, 661, 691, 907
AlwI GGATC 1 cut(s) 489
Ama87I CYCGRG 1 cut(s) 24
ApeKI GCWGC 1 cut(s) 272
ApoI RAATTY 4 cut(s) 516, 635, 805, 1131
ArsI GACNNNNNNTTYG 4 cut(s) 605, 637, 769, 801
Asp700I GAANNNNTTC 1 cut(s) 981
AspA2I CCTAGG 1 cut(s) 719
AspLEI GCGC 2 cut(s) 973, 1207
AspS9I GGNCC 3 cut(s) 421, 545, 1096
AsuC2I CCSGG 1 cut(s) 1255
AsuHPI GGTGA 1 cut(s) 515
AsuII TTCGAA 1 cut(s) 951
AvaI CYCGRG 1 cut(s) 24
AvaII GGWCC 3 cut(s) 421, 545, 1096
AvrII CCTAGG 1 cut(s) 719
BbvI GCAGC 1 cut(s) 284
BccI CCATC 4 cut(s) 41, 734, 776, 823
BciVI GTATCC 1 cut(s) 90
BclI TGATCA 1 cut(s) 3
BcnI CCSGG 1 cut(s) 1255
BcoDI GTCTC 6 cut(s) 179, 223, 240, 661, 691, 907
BfaI CTAG 2 cut(s) 230, 720
BfuI GTATCC 1 cut(s) 90
BglII AGATCT 1 cut(s) 153
BisI GCNGC 1 cut(s) 273
BlnI CCTAGG 1 cut(s) 719
BlsI GCNGC 1 cut(s) 274
Bme1390I CCNGG 1 cut(s) 1255
Bme18I GGWCC 3 cut(s) 421, 545, 1096
BmeT110I CYCGRG 1 cut(s) 24
BmgT120I GGNCC 3 cut(s) 421, 545, 1096
BmiI GGNNCC 3 cut(s) 422, 423, 455
BmrFI CCNGG 1 cut(s) 1255
BmsI GCATC 1 cut(s) 739
Bpu14I TTCGAA 1 cut(s) 951
BpuMI CCSGG 1 cut(s) 1255
BsaI GGTCTC 3 cut(s) 240, 691, 907
BsaJI CCNNGG 2 cut(s) 488, 719
BsaWI WCCGGW 2 cut(s) 70, 547
Bsc4I CCNNNNNNNGG 3 cut(s) 445, 626, 1241
Bse118I RCCGGY 1 cut(s) 275
Bse1I ACTGG 2 cut(s) 17, 391
Bse3DI GCAATG 1 cut(s) 996
BseDI CCNNGG 2 cut(s) 488, 719
BseGI GGATG 1 cut(s) 745
BseLI CCNNNNNNNGG 3 cut(s) 445, 626, 1241
BseMI GCAATG 1 cut(s) 996
BseMII CTCAG 3 cut(s) 135, 192, 702
BseNI ACTGG 2 cut(s) 17, 391
BseRI GAGGAG 1 cut(s) 661
BseXI GCAGC 1 cut(s) 284
BsiHKCI CYCGRG 1 cut(s) 24
BsiSI CCGG 4 cut(s) 71, 276, 548, 1255
BslFI GGGAC 1 cut(s) 434
BslI CCNNNNNNNGG 3 cut(s) 445, 626, 1241
BsmAI GTCTC 6 cut(s) 179, 223, 240, 661, 691, 907
BsmFI GGGAC 1 cut(s) 434
BsmI GAATGC 1 cut(s) 575
Bso31I GGTCTC 3 cut(s) 240, 691, 907
BsoBI CYCGRG 1 cut(s) 24
Bsp119I TTCGAA 1 cut(s) 951
Bsp143I GATC 5 cut(s) 3, 153, 481, 754, 1072
BspACI CCGC 1 cut(s) 439
BspCNI CTCAG 3 cut(s) 134, 193, 701
BspLI GGNNCC 3 cut(s) 422, 423, 455
BspPI GGATC 1 cut(s) 489
BspT104I TTCGAA 1 cut(s) 951
BspTNI GGTCTC 3 cut(s) 240, 691, 907
BsrBI CCGCTC 1 cut(s) 441
BsrDI GCAATG 1 cut(s) 996
BsrFI RCCGGY 1 cut(s) 275
BsrI ACTGG 2 cut(s) 17, 391
BssAI RCCGGY 1 cut(s) 275
BssECI CCNNGG 2 cut(s) 488, 719
BssMI GATC 5 cut(s) 3, 153, 481, 754, 1072
BssT1I CCWWGG 1 cut(s) 719
Bst4CI ACNGT 4 cut(s) 290, 858, 901, 1027
BstBI TTCGAA 1 cut(s) 951
BstDEI CTNAG 3 cut(s) 121, 201, 688
BstDSI CCRYGG 1 cut(s) 488
BstF5I GGATG 1 cut(s) 745
BstHHI GCGC 2 cut(s) 973, 1207
BstKTI GATC 5 cut(s) 6, 156, 484, 757, 1075
BstMAI GTCTC 6 cut(s) 179, 223, 240, 661, 691, 907
BstMBI GATC 5 cut(s) 3, 153, 481, 754, 1072
BstNSI RCATGY 2 cut(s) 351, 619
BstSCI CCNGG 1 cut(s) 1253
BstV1I GCAGC 1 cut(s) 284
BstX2I RGATCY 2 cut(s) 153, 481
BstYI RGATCY 2 cut(s) 153, 481
BsuI GTATCC 1 cut(s) 90
BtgI CCRYGG 1 cut(s) 488
BtsCI GGATG 1 cut(s) 745
CfoI GCGC 2 cut(s) 973, 1207
Cfr10I RCCGGY 1 cut(s) 275
Cfr13I GGNCC 3 cut(s) 421, 545, 1096
Csp6I GTAC 2 cut(s) 436, 1004
CspCI CAANNNNNGTGG 2 cut(s) 580, 615
CviAII CATG 9 cut(s) 304, 348, 444, 530, 616, 986, 1007, 1093, 1212
CviJI RGCY 9 cut(s) 125, 275, 297, 454, 462, 833, 892, 928, 1068
CviKI_1 RGCY 9 cut(s) 125, 275, 297, 454, 462, 833, 892, 928, 1068
CviQI GTAC 2 cut(s) 436, 1004
DdeI CTNAG 3 cut(s) 121, 201, 688
DpnI GATC 5 cut(s) 5, 155, 483, 756, 1074
DpnII GATC 5 cut(s) 3, 153, 481, 754, 1072
DraI TTTAAA 1 cut(s) 339
DrdI GACNNNNNNGTC 1 cut(s) 193
DseDI GACNNNNNNGTC 1 cut(s) 193
Eco130I CCWWGG 1 cut(s) 719
Eco31I GGTCTC 3 cut(s) 240, 691, 907
Eco47I GGWCC 3 cut(s) 421, 545, 1096
Eco57I CTGAAG 2 cut(s) 755, 996
Eco88I CYCGRG 1 cut(s) 24
EcoO109I RGGNCCY 1 cut(s) 421
EcoT14I CCWWGG 1 cut(s) 719
ErhI CCWWGG 1 cut(s) 719
FaeI CATG 9 cut(s) 307, 351, 447, 533, 619, 989, 1010, 1096, 1215
FalI AAGNNNNNCTT 2 cut(s) 876, 908
FaqI GGGAC 1 cut(s) 434
FatI CATG 9 cut(s) 303, 347, 443, 529, 615, 985, 1006, 1092, 1211
FbaI TGATCA 1 cut(s) 3
FblI GTMKAC 1 cut(s) 330
Fnu4HI GCNGC 1 cut(s) 273
FokI GGATG 1 cut(s) 752
Fsp4HI GCNGC 1 cut(s) 273
FspBI CTAG 2 cut(s) 230, 720
FspI TGCGCA 1 cut(s) 1206
GlaI GCGC 2 cut(s) 972, 1206
GluI GCNGC 1 cut(s) 273
HapII CCGG 4 cut(s) 71, 276, 548, 1255
HhaI GCGC 2 cut(s) 973, 1207
Hin1II CATG 9 cut(s) 307, 351, 447, 533, 619, 989, 1010, 1096, 1215
Hin6I GCGC 2 cut(s) 971, 1205
HinP1I GCGC 2 cut(s) 971, 1205
HincII GTYRAC 2 cut(s) 331, 405
HindII GTYRAC 2 cut(s) 331, 405
HindIII AAGCTT 1 cut(s) 460
HinfI GANTC 5 cut(s) 119, 187, 251, 411, 1231
HpaII CCGG 4 cut(s) 71, 276, 548, 1255
HphI GGTGA 1 cut(s) 515
Hpy166II GTNNAC 5 cut(s) 110, 331, 405, 545, 916
Hpy188I TCNGA 9 cut(s) 202, 393, 458, 735, 754, 765, 882, 940, 1195
Hpy188III TCNNGA 7 cut(s) 26, 97, 359, 367, 580, 810, 1115
Hpy8I GTNNAC 5 cut(s) 110, 331, 405, 545, 916
HpyAV CCTTC 3 cut(s) 174, 472, 730
HpyCH4III ACNGT 4 cut(s) 290, 858, 901, 1027
HpyCH4IV ACGT 2 cut(s) 375, 1039
HpyCH4V TGCA 5 cut(s) 351, 730, 989, 1001, 1092
HpyF3I CTNAG 3 cut(s) 121, 201, 688
HpySE526I ACGT 2 cut(s) 375, 1039
Hsp92II CATG 9 cut(s) 307, 351, 447, 533, 619, 989, 1010, 1096, 1215
HspAI GCGC 2 cut(s) 971, 1205
KflI GGGWCCC 1 cut(s) 421
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 5 cut(s) 3, 153, 481, 754, 1072
LmnI GCTCC 1 cut(s) 459
Lsp1109I GCAGC 1 cut(s) 284
LweI GCATC 1 cut(s) 739
MaeI CTAG 2 cut(s) 230, 720
MaeII ACGT 2 cut(s) 375, 1039
MaeIII GTNAC 3 cut(s) 19, 503, 1045
MalI GATC 5 cut(s) 5, 155, 483, 756, 1074
MbiI CCGCTC 1 cut(s) 441
MboI GATC 5 cut(s) 3, 153, 481, 754, 1072
MboII GAAGA 2 cut(s) 74, 705
MfeI CAATTG 1 cut(s) 1236
MflI RGATCY 2 cut(s) 153, 481
MlyI GAGTC 2 cut(s) 113, 181
MmeI TCCRAC 1 cut(s) 12
MnlI CCTC 8 cut(s) 196, 260, 393, 619, 639, 1047, 1109, 1174
MroXI GAANNNNTTC 1 cut(s) 981
MseI TTAA 6 cut(s) 338, 372, 510, 660, 1029, 1185
MslI CAYNNNNRTG 3 cut(s) 131, 448, 1141
MspA1I CMGCKG 1 cut(s) 928
MspI CCGG 4 cut(s) 71, 276, 548, 1255
MspR9I CCNGG 1 cut(s) 1255
MunI CAATTG 1 cut(s) 1236
Mva1269I GAATGC 1 cut(s) 575
NciI CCSGG 1 cut(s) 1255
NdeII GATC 5 cut(s) 3, 153, 481, 754, 1072
NlaIII CATG 9 cut(s) 307, 351, 447, 533, 619, 989, 1010, 1096, 1215
NlaIV GGNNCC 3 cut(s) 422, 423, 455
NmuCI GTSAC 1 cut(s) 503
NsbI TGCGCA 1 cut(s) 1206
NspI RCATGY 2 cut(s) 351, 619
NspV TTCGAA 1 cut(s) 951
PaeR7I CTCGAG 1 cut(s) 24
PcsI WCGNNNNNNNCGW 1 cut(s) 372
PctI GAATGC 1 cut(s) 575
PdmI GAANNNNTTC 1 cut(s) 981
PfeI GAWTC 3 cut(s) 251, 411, 1231
PflMI CCANNNNNTGG 1 cut(s) 1241
PkrI GCNGC 1 cut(s) 274
PleI GAGTC 2 cut(s) 113, 181
PpsI GAGTC 2 cut(s) 113, 181
PpuMI RGGWCCY 1 cut(s) 421
Psp5II RGGWCCY 1 cut(s) 421
PspN4I GGNNCC 3 cut(s) 422, 423, 455
PspPI GGNCC 3 cut(s) 421, 545, 1096
PspPPI RGGWCCY 1 cut(s) 421
PsuI RGATCY 2 cut(s) 153, 481
PvuII CAGCTG 1 cut(s) 928
RsaI GTAC 2 cut(s) 437, 1005
RsaNI GTAC 2 cut(s) 436, 1004
RseI CAYNNNNRTG 3 cut(s) 131, 448, 1141
SalI GTCGAC 1 cut(s) 329
SaqAI TTAA 6 cut(s) 338, 372, 510, 660, 1029, 1185
SatI GCNGC 1 cut(s) 273
Sau3AI GATC 5 cut(s) 3, 153, 481, 754, 1072
Sau96I GGNCC 3 cut(s) 421, 545, 1096
SchI GAGTC 2 cut(s) 113, 181
ScrFI CCNGG 1 cut(s) 1255
SetI ASST 8 cut(s) 207, 378, 404, 464, 930, 1042, 1128, 1185
SfaNI GCATC 1 cut(s) 739
Sfr274I CTCGAG 1 cut(s) 24
SfuI TTCGAA 1 cut(s) 951
SinI GGWCC 3 cut(s) 421, 545, 1096
SlaI CTCGAG 1 cut(s) 24
SmiMI CAYNNNNRTG 3 cut(s) 131, 448, 1141
SmlI CTYRAG 1 cut(s) 24
SmoI CTYRAG 1 cut(s) 24
SsiI CCGC 1 cut(s) 439
SspI AATATT 2 cut(s) 708, 910
SspMI CTAG 2 cut(s) 230, 720
StyD4I CCNGG 1 cut(s) 1253
StyI CCWWGG 1 cut(s) 719
TaaI ACNGT 4 cut(s) 290, 858, 901, 1027
TaiI ACGT 2 cut(s) 378, 1042
TaqI TCGA 5 cut(s) 25, 45, 330, 787, 951
TatI WGTACW 1 cut(s) 1003
TfiI GAWTC 3 cut(s) 251, 411, 1231
Tru1I TTAA 6 cut(s) 338, 372, 510, 660, 1029, 1185
Tru9I TTAA 6 cut(s) 338, 372, 510, 660, 1029, 1185
TseFI GTSAC 1 cut(s) 503
TseI GCWGC 1 cut(s) 272
Tsp45I GTSAC 1 cut(s) 503
TspDTI ATGAA 4 cut(s) 224, 528, 974, 1050
Van91I CCANNNNNTGG 1 cut(s) 1241
VpaK11BI GGWCC 3 cut(s) 421, 545, 1096
XapI RAATTY 4 cut(s) 516, 635, 805, 1131
XceI RCATGY 2 cut(s) 351, 619
XhoI CTCGAG 1 cut(s) 24
XmaJI CCTAGG 1 cut(s) 719
XmiI GTMKAC 1 cut(s) 330
XmnI GAANNNNTTC 1 cut(s) 981
XspI CTAG 2 cut(s) 230, 720
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.