Rroxscaffold_155G00443930

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000155
OG0001839
Physical Location & Seq
Forward (+)
90377 .. 97320
6944 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_155G00443930.1

Sequence Viewer

Length: 2802 bp
ATGACATACTCATTAAGAAACCCTCCTGCAAAGAGAAAATATACCCTTCAAATCCGCTATGGTGGACACTTTGAAACTGAGAGTGATCCTACTTACCAAAAATACCAAAGTTATACAGGAGAAAGAGCAATTTGGGTTGAAAATCTTGATGATTATTCGGGAAAGTCTTGTTGGAGTGATGTCAATGATCTTGCAAGTAAGCTGGGCTACCGAAATACTCGAGAAATATATAGAAGGCATAAAGGACGTCTGTTGGAAATTACTTCTCACGATGACGTAACCGAAATGATTAGTCAACTTGGAGCTAATCGAGAGTTGGTACTATATATTGTTGGACAGAGAGAAGAGGAAGCACAGGCACAAAATCCTACAATTCTATCTTGGAACTGTCGTGGACTTAACAGACCATCAACCAGGGCTATGTTGAGAGTTCTTATTTTGATTTATGATCCCACGATTATTATTCTTATAGAGACTCGCTTGTCATCCTCGGCTATTGGGAAAATCTGTTACTTTATGGGGTTCCAAAATTTTAGGGCTTCCTCTGCCAAAAGTAATGTGGCTTATCTTAAGAATTTCACTGCCAACACAAATGAGGATGAGGACCAGGATCAGGATGATTCAAATAATTGTGTTGGATTGGCAGTGTTATGGAAAGCAAATTTAAAGTTGGAGTTGTCTGATAACTCAGACAACTATCTTGATTTTGAGGTTGAGGTTCCTTGTACTGATAAAATTTGGAAGTTTACAGCATTTTATGGACACCCGACAAAAAAGGGACTCTCACGGATGTGGCTCGAGAAATTGAAATTATCAAATGATCGATCACAAAAATCTGAATGGTTAGTTGTTGGGGATCTTAATGAAATTTCCAATCAAAAAGAGAAAATGGGTGGAAACACGAAGAATCCGGCAGATATAACAAGGTTCACGGAGTGCTTGGAAAAATGTCGTCTTCAAGATTTGTCTTTTGAGGGCAATAACTATACGTGGACAGGCCCTAGAGGAGTCAAAGAGAGACTTGATAGGGGAGTGGCGTCATCGACTTGGAAAAAATTATTTCCTCATCATGCCAAGGTATTACATCTTACTGATAAAACAATCTCAGATCACATTCCTATATTGGTAGAAACTGATAGTCGAAAACAAAAGCCATTGAAGACAAAAACTCTAATGGTGAAGATGGAGACATTGCTTTCTTTTGTTGCTAAGGTGGAAAATGCATATCAGGAAACTTACCTTGCGACAGTGTCGCAAATGAAAAGTCAAATGAAGGAAATTCCAGATTTCTACTTTGACATTGCACAAAATGTGGCACCTCATATTTTTCAAGTGAAGTATATTGCAGCACAAGTACAACACAAGGATAAGGATATAGTGAAGTATGAACGGCTGGTTACTCTTACTATATGCAGTAATCTTCGTGCATTATTGTTGAATTGCATGGCCATCCCCATACCAAGCCGAGAGTATGACCAGGGCCGGCCCTCACAATCATCTCTCTATTTAGGGGGGGGAATGATTAAACCTCAAGGCATAGTAACAGAGCTTCACGGAAAAATTATTGATGAATACGAGTGTTACTTTCCTCAAAACCACGACTTTAGAAGACATTGTGAAGAAACAATTGAGTTTTTGCTGGACACTTTGGGATGGCGGCATTCGGAGAATGTTTCTGAATATGATATGACTGAAGAGCAATTAATTCAAGGACTAAATGACCTTGAAAAAAGAATGTTTATAACAAATAGAGACATCATGCTGACTGAAAGATTGGGACCATGGATAGAGTTGATAGACGACGGCACTGAAAAGGAGAAGCTTGCAGAGTTCGCTGACACGAGTATAATTGAAAGCCAAGTTGAGTTGCTAAAATATATCGGTTGCATTTCAATTTCGAAAAATAAGGAAATTGCTTTAGAAGCTGGCGAAGATCCTAAACCAATAGTCAATCGGCTTGAAAAGGCAGCAGATGAAGCCTTCAAAACGCTTAAAAATAAGTCCACAAAAACAAAGTCTATGCCTTACTCAGAATACATCTACAGGCTTAAAGAGGCATACAACTTAAGTGGGGCAGAAAAGGCTATCACTGCTACCGACTCCTTCTCCAAAACATTCATCGTTTGGCGTCAGCCTAGGATAGTCGGTTCGATCGCCTTTGCAATCCTGAATGAACTCCTTAAATCTAAGGAGATAAACGAGGAGATTATTGAAAAAGTTATTCAGTTCTACCCGAACGTCCCGGGCGCGGCGGAGAAGAACAAGGAGGATGAAGACGAGAAGTTGACGGCGATGTTAGGGCCGGAGGCCGAGGAGGAGGAGGAGACAGAGGAGATGGAGGAGGTTGAGGATCTTCCAGATCCCGAGTGTGATGATTTAGATTTTCATGTAAAGTACCTGAAGAAGTGGGCCATGGAGAGGGCCATGCAGAAGGTAAAGGAGTTAGAACCCACAGAACAAGACAGGATGGAGTTGTTGAAACATTGCAGGGAAATCGGATGCAGTTTGAAACAAGACCAAGCCCAGAAGATCCTCAATATGATGATTTCTTTCTTAAGCAATTCTGAGTTTTTTACACGATTCTCACCTTATAAAACTGATAGGAGAATCTGCATCAAAATTTTCATGGAAAAGGTCCGAGCCTATCTCGAGCGTTCTTGCATCCTGAACGACTTGGGGACGGTGTACCGCGCCATTGAGACTGTCAAGTCAATAGAAGAAGAAGAAGAATTGAAAATGGAGCGGCAGCGTGTCAGCACAATGCTAAGGCTTCAACGACGTTTTGAGTCTAGTAAAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko05134 map03013 map05134
Pfam Domains
Protein Families
GTP_EFTU GTP_EFTU_D2 GTP_EFTU_D3

Protein Analysis

933

Amino Acids

108.18

Weight (kDa)

5.64

Isoelectric Point (pI)

42.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 126 - 371 5.2e-06 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1742, 2592
AasI GACNNNNNNGTC 2 cut(s) 481, 2707
AatII GACGTC 1 cut(s) 250
AccB1I GGYRCC 1 cut(s) 1315
AccBSI CCGCTC 1 cut(s) 2743
AccII CGCG 2 cut(s) 2249, 2691
AciI CCGC 6 cut(s) 55, 1657, 2249, 2252, 2689, 2743
AclWI GGATC 8 cut(s) 80, 443, 618, 864, 1928, 2354, 2358, 2524
AcoI YGGCCR 1 cut(s) 1446
AcsI RAATTY 7 cut(s) 529, 574, 661, 735, 867, 1278, 2619
AcuI CTGAAG 2 cut(s) 1713, 2420
AcyI GRCGYC 3 cut(s) 247, 1037, 2128
AdeI CACNNNGTG 1 cut(s) 936
AfaI GTAC 5 cut(s) 321, 727, 1356, 2396, 2687
AfiI CCNNNNNNNGG 3 cut(s) 613, 2248, 2418
AflII CTTAAG 3 cut(s) 569, 2065, 2554
AjnI CCWGG 3 cut(s) 413, 606, 1476
AjuI GAANNNNNNNTTGG 2 cut(s) 154, 186
AleI CACNNNNGTG 1 cut(s) 790
AloI GAACNNNNNNTCC 2 cut(s) 2131, 2163
AluBI AGCT 5 cut(s) 202, 305, 1549, 1822, 1925
AluI AGCT 5 cut(s) 202, 305, 1549, 1822, 1925
Alw26I GTCTC 6 cut(s) 467, 1012, 1181, 1746, 2318, 2693
AlwI GGATC 8 cut(s) 80, 443, 618, 864, 1928, 2354, 2358, 2524
Ama87I CYCGRG 5 cut(s) 219, 797, 2242, 2363, 2648
AoxI GGCC 8 cut(s) 997, 1446, 1480, 1484, 2300, 2307, 2409, 2421
ApeKI GCWGC 3 cut(s) 1346, 1967, 2746
ApoI RAATTY 7 cut(s) 529, 574, 661, 735, 867, 1278, 2619
ArsI GACNNNNNNTTYG 4 cut(s) 1249, 1281, 2000, 2032
AseI ATTAAT 1 cut(s) 1703
AspA2I CCTAGG 1 cut(s) 2135
AspLEI GCGC 2 cut(s) 2249, 2693
AspS9I GGNCC 9 cut(s) 604, 998, 1480, 1485, 1778, 2300, 2409, 2421, 2635
AsuC2I CCSGG 2 cut(s) 2243, 2244
AsuHPI GGTGA 2 cut(s) 1189, 2577
AsuII TTCGAA 1 cut(s) 1898
AvaI CYCGRG 5 cut(s) 219, 797, 2242, 2363, 2648
AvaII GGWCC 3 cut(s) 604, 1778, 2635
AvrII CCTAGG 1 cut(s) 2135
BalI TGGCCA 1 cut(s) 1448
BanI GGYRCC 1 cut(s) 1315
BarI GAAGNNNNNNTAC 2 cut(s) 1533, 1565
BauI CACGAG 1 cut(s) 1840
BbsI GAAGAC 4 cut(s) 947, 1166, 1615, 2280
BbvI GCAGC 3 cut(s) 1358, 1979, 2758
BccI CCATC 6 cut(s) 415, 1177, 1457, 1647, 2329, 2461
BceAI ACGGC 3 cut(s) 1406, 1819, 2304
BcgI CGANNNNNNTGC 2 cut(s) 2476, 2510
BciT130I CCWGG 3 cut(s) 415, 608, 1478
BcnI CCSGG 2 cut(s) 2243, 2244
BcoDI GTCTC 6 cut(s) 467, 1012, 1181, 1746, 2318, 2693
BfaI CTAG 3 cut(s) 1002, 2136, 2790
BfmI CTRYAG 1 cut(s) 2041
BfrI CTTAAG 3 cut(s) 569, 2065, 2554
BisI GCNGC 6 cut(s) 1347, 1658, 1968, 2250, 2744, 2747
BlnI CCTAGG 1 cut(s) 2135
BlsI GCNGC 6 cut(s) 1348, 1659, 1969, 2251, 2745, 2748
Bme1390I CCNGG 5 cut(s) 415, 608, 1478, 2243, 2244
Bme18I GGWCC 3 cut(s) 604, 1778, 2635
BmeT110I CYCGRG 5 cut(s) 219, 797, 2242, 2363, 2648
BmgT120I GGNCC 9 cut(s) 604, 998, 1480, 1485, 1778, 2300, 2409, 2421, 2635
BmiI GGNNCC 4 cut(s) 524, 720, 1317, 1779
BmrFI CCNGG 5 cut(s) 415, 608, 1478, 2243, 2244
BmsI GCATC 3 cut(s) 2489, 2622, 2670
BpiI GAAGAC 4 cut(s) 947, 1166, 1615, 2280
BplI GAGNNNNNCTC 2 cut(s) 2631, 2663
Bpu10I CCTNAGC 2 cut(s) 1209, 2765
Bpu14I TTCGAA 1 cut(s) 1898
BpuEI CTTGAG 1 cut(s) 1515
BpuMI CCSGG 2 cut(s) 2243, 2244
Bsa29I ATCGAT 1 cut(s) 823
BsaAI YACGTR 1 cut(s) 990
BsaHI GRCGYC 3 cut(s) 247, 1037, 2128
BsaJI CCNNGG 9 cut(s) 414, 489, 1074, 1477, 1781, 2135, 2242, 2310, 2412
BsaXI ACNNNNNCTCC 2 cut(s) 2090, 2120
Bsc4I CCNNNNNNNGG 3 cut(s) 613, 2248, 2418
Bse118I RCCGGY 1 cut(s) 1482
Bse3DI GCAATG 3 cut(s) 1190, 1299, 2482
BseBI CCWGG 3 cut(s) 415, 608, 1478
BseCI ATCGAT 1 cut(s) 823
BseDI CCNNGG 9 cut(s) 414, 489, 1074, 1477, 1781, 2135, 2242, 2310, 2412
BseLI CCNNNNNNNGG 3 cut(s) 613, 2248, 2418
BseMI GCAATG 3 cut(s) 1190, 1299, 2482
BseMII CTCAG 5 cut(s) 69, 702, 1119, 2043, 2556
BseRI GAGGAG 8 cut(s) 1020, 2216, 2327, 2330, 2333, 2336, 2345, 2354
BseXI GCAGC 3 cut(s) 1358, 1979, 2758
BseYI CCCAGC 1 cut(s) 202
Bsh1236I CGCG 2 cut(s) 2249, 2691
Bsh1285I CGRYCG 1 cut(s) 2154
BshFI GGCC 8 cut(s) 999, 1448, 1482, 1486, 2302, 2309, 2411, 2423
BshNI GGYRCC 1 cut(s) 1315
BshVI ATCGAT 1 cut(s) 823
BsiEI CGRYCG 1 cut(s) 2154
BsiHKCI CYCGRG 5 cut(s) 219, 797, 2242, 2363, 2648
BsiSI CCGG 4 cut(s) 911, 1483, 2243, 2303
BslFI GGGAC 4 cut(s) 792, 1791, 2225, 2692
BslI CCNNNNNNNGG 3 cut(s) 613, 2248, 2418
BsmAI GTCTC 6 cut(s) 467, 1012, 1181, 1746, 2318, 2693
BsmFI GGGAC 4 cut(s) 792, 1791, 2225, 2692
BsmI GAATGC 1 cut(s) 1660
BsnI GGCC 8 cut(s) 999, 1448, 1482, 1486, 2302, 2309, 2411, 2423
BsoBI CYCGRG 5 cut(s) 219, 797, 2242, 2363, 2648
Bsp119I TTCGAA 1 cut(s) 1898
Bsp19I CCATGG 2 cut(s) 1781, 2412
BspACI CCGC 6 cut(s) 55, 1657, 2249, 2252, 2689, 2743
BspANI GGCC 8 cut(s) 999, 1448, 1482, 1486, 2302, 2309, 2411, 2423
BspCNI CTCAG 5 cut(s) 70, 701, 1118, 2042, 2557
BspDI ATCGAT 1 cut(s) 823
BspFNI CGCG 2 cut(s) 2249, 2691
BspLI GGNNCC 4 cut(s) 524, 720, 1317, 1779
BspPI GGATC 8 cut(s) 80, 443, 618, 864, 1928, 2354, 2358, 2524
BspQI GCTCTTC 1 cut(s) 1689
BspT104I TTCGAA 1 cut(s) 1898
BspT107I GGYRCC 1 cut(s) 1315
BspTI CTTAAG 3 cut(s) 569, 2065, 2554
BsrBI CCGCTC 1 cut(s) 2743
BsrDI GCAATG 3 cut(s) 1190, 1299, 2482
BsrFI RCCGGY 1 cut(s) 1482
BssAI RCCGGY 1 cut(s) 1482
BssECI CCNNGG 9 cut(s) 414, 489, 1074, 1477, 1781, 2135, 2242, 2310, 2412
BssNI GRCGYC 3 cut(s) 247, 1037, 2128
BssSI CACGAG 1 cut(s) 1840
BssT1I CCWWGG 4 cut(s) 1074, 1781, 2135, 2412
Bst2BI CACGAG 1 cut(s) 1840
Bst2UI CCWGG 3 cut(s) 415, 608, 1478
Bst4CI ACNGT 4 cut(s) 389, 1249, 2683, 2704
Bst6I CTCTTC 2 cut(s) 339, 1689
BstACI GRCGYC 3 cut(s) 247, 1037, 2128
BstAFI CTTAAG 3 cut(s) 569, 2065, 2554
BstBAI YACGTR 1 cut(s) 990
BstBI TTCGAA 1 cut(s) 1898
BstC8I GCNNGC 3 cut(s) 1484, 1824, 1927
BstDEI CTNAG 8 cut(s) 78, 688, 1105, 1209, 2029, 2187, 2565, 2765
BstDSI CCRYGG 2 cut(s) 1781, 2412
BstFNI CGCG 2 cut(s) 2249, 2691
BstHHI GCGC 2 cut(s) 2249, 2693
BstMAI GTCTC 6 cut(s) 467, 1012, 1181, 1746, 2318, 2693
BstMCI CGRYCG 1 cut(s) 2154
BstMWI GCNNNNNNNGC 6 cut(s) 545, 1832, 1922, 1976, 2081, 2090
BstNI CCWGG 3 cut(s) 415, 608, 1478
BstSCI CCNGG 5 cut(s) 413, 606, 1476, 2241, 2242
BstSFI CTRYAG 1 cut(s) 2041
BstUI CGCG 2 cut(s) 2249, 2691
BstV1I GCAGC 3 cut(s) 1358, 1979, 2758
BstV2I GAAGAC 4 cut(s) 947, 1166, 1615, 2280
BstX2I RGATCY 5 cut(s) 856, 1933, 2350, 2359, 2529
BstYI RGATCY 5 cut(s) 856, 1933, 2350, 2359, 2529
Bsu15I ATCGAT 1 cut(s) 823
BsuRI GGCC 8 cut(s) 999, 1448, 1482, 1486, 2302, 2309, 2411, 2423
BsuTUI ATCGAT 1 cut(s) 823
BtgI CCRYGG 2 cut(s) 1781, 2412
BtgZI GCGATG 1 cut(s) 2306
BtsI GCAGTG 3 cut(s) 579, 651, 2088
BtsIMutI CAGTG 5 cut(s) 579, 651, 1254, 1806, 2088
Cac8I GCNNGC 3 cut(s) 1484, 1824, 1927
CfoI GCGC 2 cut(s) 2249, 2693
Cfr10I RCCGGY 1 cut(s) 1482
Cfr13I GGNCC 9 cut(s) 604, 998, 1480, 1485, 1778, 2300, 2409, 2421, 2635
Cfr9I CCCGGG 1 cut(s) 2242
ClaI ATCGAT 1 cut(s) 823
CseI GACGC 2 cut(s) 1026, 2117
Csp6I GTAC 5 cut(s) 320, 726, 1355, 2395, 2686
CspCI CAANNNNNGTGG 2 cut(s) 2050, 2085
CviAII CATG 8 cut(s) 1070, 1444, 1759, 1782, 2387, 2413, 2425, 2626
CviQI GTAC 5 cut(s) 320, 726, 1355, 2395, 2686
DdeI CTNAG 8 cut(s) 78, 688, 1105, 1209, 2029, 2187, 2565, 2765
DraI TTTAAA 1 cut(s) 666
DraIII CACNNNGTG 1 cut(s) 936
DrdI GACNNNNNNGTC 2 cut(s) 481, 2707
DseDI GACNNNNNNGTC 2 cut(s) 481, 2707
EaeI YGGCCR 1 cut(s) 1446
Eam1104I CTCTTC 2 cut(s) 339, 1689
EarI CTCTTC 2 cut(s) 339, 1689
EciI GGCGGA 1 cut(s) 2267
Eco130I CCWWGG 4 cut(s) 1074, 1781, 2135, 2412
Eco47I GGWCC 3 cut(s) 604, 1778, 2635
Eco57I CTGAAG 2 cut(s) 1713, 2420
Eco88I CYCGRG 5 cut(s) 219, 797, 2242, 2363, 2648
EcoO109I RGGNCCY 1 cut(s) 998
EcoRII CCWGG 3 cut(s) 413, 606, 1476
EcoT14I CCWWGG 4 cut(s) 1074, 1781, 2135, 2412
EcoT22I ATGCAT 1 cut(s) 1225
ErhI CCWWGG 4 cut(s) 1074, 1781, 2135, 2412
FaeI CATG 8 cut(s) 1073, 1447, 1762, 1785, 2390, 2416, 2428, 2629
FalI AAGNNNNNCTT 2 cut(s) 1005, 1037
FaqI GGGAC 4 cut(s) 792, 1791, 2225, 2692
FatI CATG 8 cut(s) 1069, 1443, 1758, 1781, 2386, 2412, 2424, 2625
Fnu4HI GCNGC 6 cut(s) 1347, 1658, 1968, 2250, 2744, 2747
FseI GGCCGGCC 1 cut(s) 1486
Fsp4HI GCNGC 6 cut(s) 1347, 1658, 1968, 2250, 2744, 2747
FspBI CTAG 3 cut(s) 1002, 2136, 2790
GlaI GCGC 2 cut(s) 2248, 2692
GluI GCNGC 6 cut(s) 1347, 1658, 1968, 2250, 2744, 2747
GsaI CCCAGC 1 cut(s) 206
HaeIII GGCC 8 cut(s) 999, 1448, 1482, 1486, 2302, 2309, 2411, 2423
HapII CCGG 4 cut(s) 911, 1483, 2243, 2303
HgaI GACGC 2 cut(s) 1026, 2117
HhaI GCGC 2 cut(s) 2249, 2693
Hin1I GRCGYC 3 cut(s) 247, 1037, 2128
Hin1II CATG 8 cut(s) 1073, 1447, 1762, 1785, 2390, 2416, 2428, 2629
Hin6I GCGC 2 cut(s) 2247, 2691
HinP1I GCGC 2 cut(s) 2247, 2691
HincII GTYRAC 2 cut(s) 296, 2286
HindII GTYRAC 2 cut(s) 296, 2286
HindIII AAGCTT 1 cut(s) 1820
HinfI GANTC 9 cut(s) 475, 620, 780, 907, 1008, 2099, 2580, 2607, 2786
HpaII CCGG 4 cut(s) 911, 1483, 2243, 2303
HphI GGTGA 2 cut(s) 1189, 2577
Hpy166II GTNNAC 9 cut(s) 65, 296, 395, 747, 930, 993, 2004, 2286, 2686
Hpy8I GTNNAC 9 cut(s) 65, 296, 395, 747, 930, 993, 2004, 2286, 2686
Hpy99I CGWCG 2 cut(s) 1805, 2781
HpyAV CCTTC 6 cut(s) 56, 228, 1267, 1990, 2113, 2425
HpyCH4III ACNGT 4 cut(s) 389, 1249, 2683, 2704
HpyCH4IV ACGT 5 cut(s) 247, 276, 989, 2238, 2779
HpyF10VI GCNNNNNNNGC 6 cut(s) 545, 1832, 1922, 1976, 2081, 2090
HpyF3I CTNAG 8 cut(s) 78, 688, 1105, 1209, 2029, 2187, 2565, 2765
HpySE526I ACGT 5 cut(s) 247, 276, 989, 2238, 2779
Hsp92I GRCGYC 3 cut(s) 247, 1037, 2128
Hsp92II CATG 8 cut(s) 1073, 1447, 1762, 1785, 2390, 2416, 2428, 2629
HspAI GCGC 2 cut(s) 2247, 2691
KroI GCCGGC 1 cut(s) 1482
KroNI GCCGGC 1 cut(s) 1484
LguI GCTCTTC 1 cut(s) 1689
LmnI GCTCC 2 cut(s) 302, 2740
Lsp1109I GCAGC 3 cut(s) 1358, 1979, 2758
LweI GCATC 3 cut(s) 2489, 2622, 2670
MaeI CTAG 3 cut(s) 1002, 2136, 2790
MaeII ACGT 5 cut(s) 247, 276, 989, 2238, 2779
MaeIII GTNAC 5 cut(s) 277, 509, 1396, 1540, 1580
MbiI CCGCTC 1 cut(s) 2743
MfeI CAATTG 1 cut(s) 1626
MflI RGATCY 5 cut(s) 856, 1933, 2350, 2359, 2529
MlsI TGGCCA 1 cut(s) 1448
MluNI TGGCCA 1 cut(s) 1448
MlyI GAGTC 5 cut(s) 469, 774, 1017, 2093, 2795
MmeI TCCRAC 5 cut(s) 152, 234, 313, 616, 651
Mox20I TGGCCA 1 cut(s) 1448
Mph1103I ATGCAT 1 cut(s) 1225
MroNI GCCGGC 1 cut(s) 1482
MscI TGGCCA 1 cut(s) 1448
MslI CAYNNNNRTG 1 cut(s) 790
Msp20I TGGCCA 1 cut(s) 1448
MspCI CTTAAG 3 cut(s) 569, 2065, 2554
MspI CCGG 4 cut(s) 911, 1483, 2243, 2303
MspR9I CCNGG 5 cut(s) 415, 608, 1478, 2243, 2244
MunI CAATTG 1 cut(s) 1626
Mva1269I GAATGC 1 cut(s) 1660
MvaI CCWGG 3 cut(s) 415, 608, 1478
MvnI CGCG 2 cut(s) 2249, 2691
MwoI GCNNNNNNNGC 6 cut(s) 545, 1832, 1922, 1976, 2081, 2090
NaeI GCCGGC 1 cut(s) 1484
NciI CCSGG 2 cut(s) 2243, 2244
NcoI CCATGG 2 cut(s) 1781, 2412
NgoMIV GCCGGC 1 cut(s) 1482
NlaIII CATG 8 cut(s) 1073, 1447, 1762, 1785, 2390, 2416, 2428, 2629
NlaIV GGNNCC 4 cut(s) 524, 720, 1317, 1779
NmeAIII GCCGAG 3 cut(s) 470, 1490, 2335
NsiI ATGCAT 1 cut(s) 1225
NspV TTCGAA 1 cut(s) 1898
OliI CACNNNNGTG 1 cut(s) 790
PaeR7I CTCGAG 3 cut(s) 219, 797, 2648
PciSI GCTCTTC 1 cut(s) 1689
PctI GAATGC 1 cut(s) 1660
PdiI GCCGGC 1 cut(s) 1484
PfeI GAWTC 4 cut(s) 620, 907, 2580, 2607
PflFI GACNNNGTC 1 cut(s) 1249
PkrI GCNGC 6 cut(s) 1348, 1659, 1969, 2251, 2745, 2748
Ple19I CGATCG 1 cut(s) 2154
PleI GAGTC 5 cut(s) 469, 774, 1016, 2093, 2794
PpsI GAGTC 5 cut(s) 469, 774, 1016, 2093, 2794
Ppu21I YACGTR 1 cut(s) 990
PshBI ATTAAT 1 cut(s) 1703
PsiI TTATAA 2 cut(s) 1742, 2592
Psp6I CCWGG 3 cut(s) 413, 606, 1476
PspFI CCCAGC 1 cut(s) 202
PspGI CCWGG 3 cut(s) 413, 606, 1476
PspN4I GGNNCC 4 cut(s) 524, 720, 1317, 1779
PspPI GGNCC 9 cut(s) 604, 998, 1480, 1485, 1778, 2300, 2409, 2421, 2635
PsuI RGATCY 5 cut(s) 856, 1933, 2350, 2359, 2529
PsyI GACNNNGTC 1 cut(s) 1249
PvuI CGATCG 1 cut(s) 2154
RigI GGCCGGCC 1 cut(s) 1486
RsaI GTAC 5 cut(s) 321, 727, 1356, 2396, 2687
RsaNI GTAC 5 cut(s) 320, 726, 1355, 2395, 2686
RseI CAYNNNNRTG 1 cut(s) 790
SapI GCTCTTC 1 cut(s) 1689
SatI GCNGC 6 cut(s) 1347, 1658, 1968, 2250, 2744, 2747
Sau96I GGNCC 9 cut(s) 604, 998, 1480, 1485, 1778, 2300, 2409, 2421, 2635
SchI GAGTC 5 cut(s) 469, 774, 1017, 2093, 2795
ScrFI CCNGG 5 cut(s) 415, 608, 1478, 2243, 2244
SfaNI GCATC 3 cut(s) 2489, 2622, 2670
SfcI CTRYAG 1 cut(s) 2041
Sfr274I CTCGAG 3 cut(s) 219, 797, 2648
SfuI TTCGAA 1 cut(s) 1898
SinI GGWCC 3 cut(s) 604, 1778, 2635
SlaI CTCGAG 3 cut(s) 219, 797, 2648
SmaI CCCGGG 1 cut(s) 2244
SmiMI CAYNNNNRTG 1 cut(s) 790
SmlI CTYRAG 7 cut(s) 219, 569, 797, 1530, 2065, 2554, 2648
SmoI CTYRAG 7 cut(s) 219, 569, 797, 1530, 2065, 2554, 2648
SsiI CCGC 6 cut(s) 55, 1657, 2249, 2252, 2689, 2743
SspMI CTAG 3 cut(s) 1002, 2136, 2790
StyD4I CCNGG 5 cut(s) 413, 606, 1476, 2241, 2242
StyI CCWWGG 4 cut(s) 1074, 1781, 2135, 2412
TaaI ACNGT 4 cut(s) 389, 1249, 2683, 2704
TaiI ACGT 5 cut(s) 250, 279, 992, 2241, 2782
TaqI TCGA 9 cut(s) 220, 310, 798, 823, 1043, 1141, 1898, 2150, 2649
TatI WGTACW 2 cut(s) 725, 1354
TauI GCSGC 3 cut(s) 1660, 2252, 2746
TfiI GAWTC 4 cut(s) 620, 907, 2580, 2607
TscAI CASTG 5 cut(s) 586, 651, 1254, 1813, 2095
TseI GCWGC 3 cut(s) 1346, 1967, 2746
TspGWI ACGGA 3 cut(s) 802, 947, 1569
TspMI CCCGGG 1 cut(s) 2242
TspRI CASTG 5 cut(s) 586, 651, 1254, 1813, 2095
Tth111I GACNNNGTC 1 cut(s) 1249
Vha464I CTTAAG 3 cut(s) 569, 2065, 2554
VpaK11BI GGWCC 3 cut(s) 604, 1778, 2635
VspI ATTAAT 1 cut(s) 1703
XapI RAATTY 7 cut(s) 529, 574, 661, 735, 867, 1278, 2619
XcmI CCANNNNNNNNNTGG 1 cut(s) 556
XhoI CTCGAG 3 cut(s) 219, 797, 2648
XmaI CCCGGG 1 cut(s) 2242
XmaJI CCTAGG 1 cut(s) 2135
XspI CTAG 3 cut(s) 1002, 2136, 2790
ZraI GACGTC 1 cut(s) 248
Zsp2I ATGCAT 1 cut(s) 1225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.