MD00G1068800.v1.1

Transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0000792
Physical Location & Seq
Reverse (-)
13493469 .. 13495817
2349 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1068800.v1.1.491

Sequence Viewer

Length: 1083 bp
ATGGACATGATCTCATCAGCAGAATGGGAAATGGAAGATCCCACTTTTATCAATCAATACGAAATGAGCTCTCTGGACTACTCATTTGAAGAGCTCATATTCCCGTCTCTCTCATCTGATAGCTACTGTTCCAATCCGAATTTCACATCGAAAGCCACAGCTGCTACACACAACTTCAGCAAAGCATTCATTGAAAATCCCCATCAGACCGGCACTCAGGGAAGGTCAGCAAAACAGCCTAAGAACACTAATAGTTGGAAATCTTGCTCCACTGACCACATAATTGCTGCCAAAGCTTCTTCTTCTTCCTCGTCACACCTAATTTCATTTGAGAACTCCGACTCATCACCTCCAACCACTTCTCAGCAGTACTATGGTCTAGATTGCAAAGTTATCAAGCCAAAGAATGAGGTGGAATATTCTAATGGAAAATTGAACCCTTCAGCTTTGGCTTCCCAATGTTCCTATGACACCCAAATTTGTTCACCCAAACATGGACAAGGGATCAAGAGGGCAGCTACGGTGACTAGAAGTCCTTTACATGCTCAAGATCATGTTCTTGCTGAGAGAAAGCGCCGAGAAAAGCTCAGCCAGCGGTTCATTGCTCTATCTGCCTTAATTCCAGGACTAAAGAAGATGGACAAGGCTTCGGTCCTCGGAGATGCGATCAAGTACGTGAAACATCTTCAAGAACGTACGAAGGTGCTGGAGGAACAAGCAGCCAAGAAAACTGGGGAAGCAGTGGTTTTTGTGAAGAGGATGCAGTACTCAGCGGATGATGATATATCTTCATCCGACGAGAACTCCGAGAGCTGCTCTGACCAGCCACTGCCAGAAATTGAAGCAAGAGTTTCGGACAAGGAGGTTCTCATACGAATCCACTGCGAGAAAACCAAAGGATGTTTGACAAGCATACTAAGTGAAATAGAAAAGCTTGGTCTCACCATAGTTCACAGCTGTGCCTTGCCCTTTGGCAATTCAACTCTTGATATCACTGTAGTTGCTCAGATGGATGTTGAATTCAGAGTGGCAGGGAAACATCTGATAAAAAACCTAAGACATGCTTTGTTCAAGTTGGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

361

Amino Acids

39.89

Weight (kDa)

6.86

Isoelectric Point (pI)

45.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 185 - 230 7.5e-11 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 595, 773
AclWI GGATC 2 cut(s) 32, 512
AcsI RAATTY 3 cut(s) 139, 477, 1019
AcuI CTGAAG 2 cut(s) 160, 426
AfaI GTAC 4 cut(s) 371, 674, 697, 767
AfiI CCNNNNNNNGG 1 cut(s) 494
AgsI TTSAA 8 cut(s) 89, 194, 436, 689, 842, 981, 1019, 1072
AhdI GACNNNNNGTC 1 cut(s) 531
AjnI CCWGG 1 cut(s) 622
AjuI GAANNNNNNNTTGG 2 cut(s) 125, 157
AleI CACNNNNGTG 1 cut(s) 957
Alw21I GWGCWC 2 cut(s) 71, 96
Alw26I GTCTC 2 cut(s) 111, 944
AlwI GGATC 2 cut(s) 32, 512
AlwNI CAGNNNCTG 1 cut(s) 829
ApeKI GCWGC 5 cut(s) 161, 287, 515, 719, 813
ApoI RAATTY 3 cut(s) 139, 477, 1019
ArsI GACNNNNNNTTYG 2 cut(s) 68, 100
AspLEI GCGC 1 cut(s) 576
AspS9I GGNCC 1 cut(s) 652
AsuHPI GGTGA 4 cut(s) 339, 477, 535, 934
AvaII GGWCC 1 cut(s) 652
BanII GRGCYC 2 cut(s) 71, 96
Bbv12I GWGCWC 2 cut(s) 71, 96
BbvI GCAGC 5 cut(s) 148, 274, 527, 731, 800
BccI CCATC 3 cut(s) 210, 631, 1003
BcgI CGANNNNNNTGC 4 cut(s) 834, 864, 868, 898
BciT130I CCWGG 1 cut(s) 624
BcoDI GTCTC 2 cut(s) 111, 944
BfaI CTAG 2 cut(s) 380, 528
BfmI CTRYAG 1 cut(s) 996
BfoI RGCGCY 1 cut(s) 577
BisI GCNGC 5 cut(s) 162, 288, 516, 720, 814
BlpI GCTNAGC 1 cut(s) 587
BlsI GCNGC 5 cut(s) 163, 289, 517, 721, 815
BmcAI AGTACT 2 cut(s) 371, 767
Bme1390I CCNGG 1 cut(s) 624
Bme18I GGWCC 1 cut(s) 652
BmeRI GACNNNNNGTC 1 cut(s) 531
BmgT120I GGNCC 1 cut(s) 652
BmrFI CCNGG 1 cut(s) 624
BmrI ACTGGG 1 cut(s) 741
BmsI GCATC 2 cut(s) 652, 750
BmuI ACTGGG 1 cut(s) 741
BplI GAGNNNNNCTC 4 cut(s) 570, 602, 800, 832
BpmI CTGGAG 1 cut(s) 728
Bpu1102I GCTNAGC 1 cut(s) 587
BpuEI CTTGAG 1 cut(s) 531
BsaAI YACGTR 1 cut(s) 676
BsaI GGTCTC 1 cut(s) 944
BsaJI CCNNGG 1 cut(s) 655
BsaXI ACNNNNNCTCC 2 cut(s) 788, 818
Bsc4I CCNNNNNNNGG 1 cut(s) 494
Bse118I RCCGGY 1 cut(s) 209
Bse1I ACTGG 1 cut(s) 736
Bse3DI GCAATG 1 cut(s) 600
BseBI CCWGG 1 cut(s) 624
BseDI CCNNGG 1 cut(s) 655
BseGI GGATG 5 cut(s) 765, 781, 791, 905, 1018
BseLI CCNNNNNNNGG 1 cut(s) 494
BseMI GCAATG 1 cut(s) 600
BseMII CTCAG 6 cut(s) 230, 377, 555, 601, 783, 1019
BseNI ACTGG 1 cut(s) 736
BseXI GCAGC 5 cut(s) 148, 274, 527, 731, 800
BsiHKAI GWGCWC 2 cut(s) 71, 96
BsiSI CCGG 1 cut(s) 210
BsiWI CGTACG 1 cut(s) 695
BslI CCNNNNNNNGG 1 cut(s) 494
BsmAI GTCTC 2 cut(s) 111, 944
BsmBI CGTCTC 1 cut(s) 111
BsmI GAATGC 1 cut(s) 185
Bso31I GGTCTC 1 cut(s) 944
Bsp1286I GDGCHC 2 cut(s) 71, 96
Bsp143I GATC 5 cut(s) 9, 37, 504, 550, 666
Bsp1720I GCTNAGC 1 cut(s) 587
BspACI CCGC 2 cut(s) 595, 773
BspCNI CTCAG 6 cut(s) 229, 376, 556, 600, 782, 1018
BspPI GGATC 2 cut(s) 32, 512
BspQI GCTCTTC 1 cut(s) 84
BspTNI GGTCTC 1 cut(s) 944
BsrDI GCAATG 1 cut(s) 600
BsrFI RCCGGY 1 cut(s) 209
BsrI ACTGG 1 cut(s) 736
BssAI RCCGGY 1 cut(s) 209
BssECI CCNNGG 1 cut(s) 655
BssMI GATC 5 cut(s) 9, 37, 504, 550, 666
Bst2UI CCWGG 1 cut(s) 624
Bst4CI ACNGT 3 cut(s) 128, 523, 997
Bst6I CTCTTC 2 cut(s) 84, 749
BstBAI YACGTR 1 cut(s) 676
BstC8I GCNNGC 1 cut(s) 593
BstDEI CTNAG 9 cut(s) 216, 240, 363, 564, 587, 769, 917, 1005, 1055
BstF5I GGATG 5 cut(s) 765, 781, 791, 905, 1018
BstH2I RGCGCY 1 cut(s) 577
BstHHI GCGC 1 cut(s) 576
BstKTI GATC 5 cut(s) 12, 40, 507, 553, 669
BstMAI GTCTC 2 cut(s) 111, 944
BstMBI GATC 5 cut(s) 9, 37, 504, 550, 666
BstMWI GCNNNNNNNGC 4 cut(s) 161, 293, 592, 611
BstNI CCWGG 1 cut(s) 624
BstNSI RCATGY 2 cut(s) 545, 1064
BstSCI CCNGG 1 cut(s) 622
BstSFI CTRYAG 1 cut(s) 996
BstV1I GCAGC 5 cut(s) 148, 274, 527, 731, 800
BstX2I RGATCY 1 cut(s) 37
BstYI RGATCY 1 cut(s) 37
BtsCI GGATG 5 cut(s) 765, 781, 791, 905, 1018
BtsI GCAGTG 3 cut(s) 747, 827, 880
BtsIMutI CAGTG 5 cut(s) 270, 747, 827, 880, 993
Cac8I GCNNGC 1 cut(s) 593
CaiI CAGNNNCTG 1 cut(s) 829
CfoI GCGC 1 cut(s) 576
Cfr10I RCCGGY 1 cut(s) 209
Cfr13I GGNCC 1 cut(s) 652
Csp6I GTAC 4 cut(s) 370, 673, 696, 766
CviAII CATG 5 cut(s) 7, 494, 542, 554, 1061
CviQI GTAC 4 cut(s) 370, 673, 696, 766
DdeI CTNAG 9 cut(s) 216, 240, 363, 564, 587, 769, 917, 1005, 1055
DpnI GATC 5 cut(s) 11, 39, 506, 552, 668
DpnII GATC 5 cut(s) 9, 37, 504, 550, 666
DriI GACNNNNNGTC 1 cut(s) 531
Eam1104I CTCTTC 2 cut(s) 84, 749
Eam1105I GACNNNNNGTC 1 cut(s) 531
EarI CTCTTC 2 cut(s) 84, 749
Ecl136II GAGCTC 2 cut(s) 69, 94
Eco24I GRGCYC 2 cut(s) 71, 96
Eco31I GGTCTC 1 cut(s) 944
Eco32I GATATC 1 cut(s) 991
Eco47I GGWCC 1 cut(s) 652
Eco53kI GAGCTC 2 cut(s) 69, 94
Eco57I CTGAAG 2 cut(s) 160, 426
EcoICRI GAGCTC 2 cut(s) 69, 94
EcoRI GAATTC 1 cut(s) 1019
EcoRII CCWGG 1 cut(s) 622
EcoRV GATATC 1 cut(s) 991
EcoT38I GRGCYC 2 cut(s) 71, 96
Esp3I CGTCTC 1 cut(s) 111
FaeI CATG 5 cut(s) 10, 497, 545, 557, 1064
FalI AAGNNNNNCTT 2 cut(s) 1048, 1080
FatI CATG 5 cut(s) 6, 493, 541, 553, 1060
Fnu4HI GCNGC 5 cut(s) 162, 288, 516, 720, 814
FokI GGATG 5 cut(s) 772, 778, 788, 912, 1025
FriOI GRGCYC 2 cut(s) 71, 96
Fsp4HI GCNGC 5 cut(s) 162, 288, 516, 720, 814
FspBI CTAG 2 cut(s) 380, 528
GlaI GCGC 1 cut(s) 575
GluI GCNGC 5 cut(s) 162, 288, 516, 720, 814
GsuI CTGGAG 1 cut(s) 728
HaeII RGCGCY 1 cut(s) 577
HapII CCGG 1 cut(s) 210
HhaI GCGC 1 cut(s) 576
Hin1II CATG 5 cut(s) 10, 497, 545, 557, 1064
Hin6I GCGC 1 cut(s) 574
HinP1I GCGC 1 cut(s) 574
HindIII AAGCTT 2 cut(s) 294, 932
HinfI GANTC 2 cut(s) 341, 876
HpaII CCGG 1 cut(s) 210
HphI GGTGA 4 cut(s) 339, 477, 535, 934
Hpy166II GTNNAC 2 cut(s) 485, 952
Hpy188III TCNNGA 6 cut(s) 74, 380, 508, 548, 689, 986
Hpy8I GTNNAC 2 cut(s) 485, 952
Hpy99I CGWCG 1 cut(s) 800
HpyAV CCTTC 3 cut(s) 216, 450, 694
HpyCH4III ACNGT 3 cut(s) 128, 523, 997
HpyCH4IV ACGT 2 cut(s) 675, 694
HpyCH4V TGCA 2 cut(s) 387, 763
HpyF10VI GCNNNNNNNGC 4 cut(s) 161, 293, 592, 611
HpyF3I CTNAG 9 cut(s) 216, 240, 363, 564, 587, 769, 917, 1005, 1055
HpySE526I ACGT 2 cut(s) 675, 694
Hsp92II CATG 5 cut(s) 10, 497, 545, 557, 1064
HspAI GCGC 1 cut(s) 574
Kzo9I GATC 5 cut(s) 9, 37, 504, 550, 666
LguI GCTCTTC 1 cut(s) 84
LmnI GCTCC 1 cut(s) 272
Lsp1109I GCAGC 5 cut(s) 148, 274, 527, 731, 800
LweI GCATC 2 cut(s) 652, 750
MaeI CTAG 2 cut(s) 380, 528
MaeII ACGT 2 cut(s) 675, 694
MaeIII GTNAC 2 cut(s) 312, 523
MalI GATC 5 cut(s) 11, 39, 506, 552, 668
MboI GATC 5 cut(s) 9, 37, 504, 550, 666
MboII GAAGA 9 cut(s) 47, 101, 291, 294, 297, 646, 677, 766, 780
MflI RGATCY 1 cut(s) 37
MhlI GDGCHC 2 cut(s) 71, 96
MluCI AATT 9 cut(s) 139, 282, 321, 431, 477, 618, 837, 976, 1019
MlyI GAGTC 1 cut(s) 335
MmeI TCCRAC 4 cut(s) 236, 363, 377, 819
MnlI CCTC 8 cut(s) 319, 360, 403, 504, 665, 703, 750, 856
MseI TTAA 1 cut(s) 617
MslI CAYNNNNRTG 1 cut(s) 957
MspA1I CMGCKG 4 cut(s) 161, 595, 773, 957
MspI CCGG 1 cut(s) 210
MspR9I CCNGG 1 cut(s) 624
Mva1269I GAATGC 1 cut(s) 185
MvaI CCWGG 1 cut(s) 624
MwoI GCNNNNNNNGC 4 cut(s) 161, 293, 592, 611
NdeII GATC 5 cut(s) 9, 37, 504, 550, 666
NlaIII CATG 5 cut(s) 10, 497, 545, 557, 1064
NmeAIII GCCGAG 1 cut(s) 602
NmuCI GTSAC 2 cut(s) 312, 523
NspI RCATGY 2 cut(s) 545, 1064
OliI CACNNNNGTG 1 cut(s) 957
PciSI GCTCTTC 1 cut(s) 84
PcsI WCGNNNNNNNCGW 1 cut(s) 804
PctI GAATGC 1 cut(s) 185
PfeI GAWTC 1 cut(s) 876
Pfl23II CGTACG 1 cut(s) 695
PfoI TCCNGGA 1 cut(s) 622
PkrI GCNGC 5 cut(s) 163, 289, 517, 721, 815
PleI GAGTC 1 cut(s) 335
PpsI GAGTC 1 cut(s) 335
Ppu21I YACGTR 1 cut(s) 676
Psp124BI GAGCTC 2 cut(s) 71, 96
Psp6I CCWGG 1 cut(s) 622
PspGI CCWGG 1 cut(s) 622
PspLI CGTACG 1 cut(s) 695
PspPI GGNCC 1 cut(s) 652
PstNI CAGNNNCTG 1 cut(s) 829
PsuI RGATCY 1 cut(s) 37
PvuII CAGCTG 2 cut(s) 161, 957
RsaI GTAC 4 cut(s) 371, 674, 697, 767
RsaNI GTAC 4 cut(s) 370, 673, 696, 766
RseI CAYNNNNRTG 1 cut(s) 957
SacI GAGCTC 2 cut(s) 71, 96
SapI GCTCTTC 1 cut(s) 84
SaqAI TTAA 1 cut(s) 617
SatI GCNGC 5 cut(s) 162, 288, 516, 720, 814
Sau3AI GATC 5 cut(s) 9, 37, 504, 550, 666
Sau96I GGNCC 1 cut(s) 652
ScaI AGTACT 2 cut(s) 371, 767
SchI GAGTC 1 cut(s) 335
ScrFI CCNGG 1 cut(s) 624
SduI GDGCHC 2 cut(s) 71, 96
SfaNI GCATC 2 cut(s) 652, 750
SfcI CTRYAG 1 cut(s) 996
SinI GGWCC 1 cut(s) 652
SmiMI CAYNNNNRTG 1 cut(s) 957
SmlI CTYRAG 1 cut(s) 546
SmoI CTYRAG 1 cut(s) 546
Sse9I AATT 9 cut(s) 139, 282, 321, 431, 477, 618, 837, 976, 1019
SsiI CCGC 2 cut(s) 595, 773
SspI AATATT 1 cut(s) 419
SspMI CTAG 2 cut(s) 380, 528
SstI GAGCTC 2 cut(s) 71, 96
StyD4I CCNGG 1 cut(s) 622
TaaI ACNGT 3 cut(s) 128, 523, 997
TaiI ACGT 2 cut(s) 678, 697
TaqI TCGA 1 cut(s) 149
TaqII GACCGA 1 cut(s) 640
TasI AATT 9 cut(s) 139, 282, 321, 431, 477, 618, 837, 976, 1019
TatI WGTACW 2 cut(s) 369, 765
TfiI GAWTC 1 cut(s) 876
Tru1I TTAA 1 cut(s) 617
Tru9I TTAA 1 cut(s) 617
TscAI CASTG 5 cut(s) 277, 747, 834, 887, 1000
TseFI GTSAC 2 cut(s) 312, 523
TseI GCWGC 5 cut(s) 161, 287, 515, 719, 813
Tsp45I GTSAC 2 cut(s) 312, 523
TspDTI ATGAA 4 cut(s) 178, 315, 589, 780
TspRI CASTG 5 cut(s) 277, 747, 834, 887, 1000
VpaK11BI GGWCC 1 cut(s) 652
XapI RAATTY 3 cut(s) 139, 477, 1019
XbaI TCTAGA 1 cut(s) 379
XceI RCATGY 2 cut(s) 545, 1064
XspI CTAG 2 cut(s) 380, 528
ZrmI AGTACT 2 cut(s) 371, 767
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.