MD01G1047400.v1.1

Transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0000792
Physical Location & Seq
Forward (+)
14932026 .. 14933451
1426 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1047400.v1.1.491

Sequence Viewer

Length: 1053 bp
ATGGAAGATCCCACTTTTATCAATCAATACGCAATGAGCTCTCTGGACTACTCACTTGATGAGCTCATTTTCTCGCCTTTCTCATCTGAGAGTTACTCATCCTACCCAAATTTCACCTCGAAAACCGCAGCTGTTACACACAACTTCAGCAAAGCATTCATTGAAAATCCCCATCAGACCGGCAGTCAGGAAAGGCCAGCAAAACAGCCTAAGAACACCACTAGTTGGAAATCTTGCTCCACTGACCACATAATTGCTGCCAAAGCTTCTTCTTCTTCCTCGTCACACCTAATTTCGTTTGAGAACTCCGACTCATCACCTCCGACCACTTCTCAGCAGTACAATGGTCTAGATTGCAAAGTTATCAAGCCAAAGAATGAGGTGGAATATTCCAATGGAAAATTGAACCTTCCAGCTTTGATTTCCCAAGGTTCCTATGACACCCAAACTTGTTCACCCAAACATGGACGAGGGATCAAGAGGGCAGCTACGGTGACTAGAAGTCCTTTACATGCTCAAGATCATGTTCTTGCTGAGAGAAAGCGCCGAGAAAAGCTCAGCCAGCGGTTCATTGCTCTATCTGCCTTACTTCCAGGCCTAAAGAAGATGGACAAGGCTTCGGTCCTCGGAGATGCAATCAAGTACGTGAAACATCTTCAAGAACGTACGAAGGTGCTGGAGGAACAAGCAGCCAAGAAAACTGGGGAAGCAGTGGTTTTTGTGAAGAGGATGCAGTACTCAGCGGATGATGATATATCTTCATCTGACGAGAACTCCGAGAGCTGCTCTGACCAGCCACTGCCAGAAATTGAAGCAAGAGTTTCGGACAAGGAGGTTCTCATACGAATCCACTGCGAGAAAACCAAAGGATGTTTGACAAGCATACTAAGTGAAATAGAAAAGCTTGGTCTCACCATAGTTCACAGCTGTGCCTTGCCCTTTGGCAATTCAACTCTTGATATCACTGTAGTTGCTCAGATGGATGCTCAATTCAGCATGACAGTGAAAGATCTGGTAAAAAACCTAAGACATGCTTTGCTCAAGTTGGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

351

Amino Acids

38.69

Weight (kDa)

8.06

Isoelectric Point (pI)

50.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 175 - 220 9.7e-11 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 225
AciI CCGC 3 cut(s) 126, 565, 743
AclWI GGATC 2 cut(s) 2, 482
AcsI RAATTY 1 cut(s) 109
AcuI CTGAAG 1 cut(s) 130
AfaI GTAC 4 cut(s) 341, 644, 667, 737
AfiI CCNNNNNNNGG 2 cut(s) 225, 464
AgsI TTSAA 5 cut(s) 164, 406, 659, 812, 951
AhdI GACNNNNNGTC 2 cut(s) 183, 501
AhlI ACTAGT 1 cut(s) 221
AjnI CCWGG 1 cut(s) 592
AleI CACNNNNGTG 1 cut(s) 927
Alw21I GWGCWC 2 cut(s) 41, 66
Alw26I GTCTC 1 cut(s) 914
AlwI GGATC 2 cut(s) 2, 482
AlwNI CAGNNNCTG 1 cut(s) 799
AoxI GGCC 2 cut(s) 194, 595
ApeKI GCWGC 5 cut(s) 128, 257, 485, 689, 783
ApoI RAATTY 1 cut(s) 109
AspLEI GCGC 1 cut(s) 546
AspS9I GGNCC 1 cut(s) 622
AsuHPI GGTGA 5 cut(s) 106, 309, 447, 505, 904
AvaII GGWCC 1 cut(s) 622
BanII GRGCYC 2 cut(s) 41, 66
Bbv12I GWGCWC 2 cut(s) 41, 66
BbvI GCAGC 5 cut(s) 140, 244, 497, 701, 770
BccI CCATC 3 cut(s) 180, 601, 973
BcgI CGANNNNNNTGC 4 cut(s) 804, 834, 838, 868
BciT130I CCWGG 1 cut(s) 594
BcoDI GTCTC 1 cut(s) 914
BcuI ACTAGT 1 cut(s) 221
BfaI CTAG 3 cut(s) 222, 350, 498
BfmI CTRYAG 1 cut(s) 966
BfoI RGCGCY 1 cut(s) 547
BglII AGATCT 1 cut(s) 1009
BisI GCNGC 5 cut(s) 129, 258, 486, 690, 784
BlpI GCTNAGC 1 cut(s) 557
BlsI GCNGC 5 cut(s) 130, 259, 487, 691, 785
BmcAI AGTACT 1 cut(s) 737
Bme1390I CCNGG 1 cut(s) 594
Bme18I GGWCC 1 cut(s) 622
BmeRI GACNNNNNGTC 2 cut(s) 183, 501
BmgT120I GGNCC 1 cut(s) 622
BmiI GGNNCC 1 cut(s) 433
BmrFI CCNGG 1 cut(s) 594
BmrI ACTGGG 1 cut(s) 711
BmsI GCATC 3 cut(s) 622, 720, 973
BmuI ACTGGG 1 cut(s) 711
BplI GAGNNNNNCTC 6 cut(s) 80, 112, 540, 572, 770, 802
BpmI CTGGAG 1 cut(s) 698
Bpu1102I GCTNAGC 1 cut(s) 557
BpuEI CTTGAG 2 cut(s) 501, 1025
BsaAI YACGTR 1 cut(s) 646
BsaI GGTCTC 1 cut(s) 914
BsaJI CCNNGG 2 cut(s) 427, 625
BsaXI ACNNNNNCTCC 2 cut(s) 758, 788
Bsc4I CCNNNNNNNGG 2 cut(s) 225, 464
Bse118I RCCGGY 1 cut(s) 179
Bse1I ACTGG 1 cut(s) 706
Bse3DI GCAATG 2 cut(s) 39, 570
BseBI CCWGG 1 cut(s) 594
BseDI CCNNGG 2 cut(s) 427, 625
BseGI GGATG 5 cut(s) 98, 735, 751, 875, 988
BseLI CCNNNNNNNGG 2 cut(s) 225, 464
BseMI GCAATG 2 cut(s) 39, 570
BseMII CTCAG 6 cut(s) 78, 347, 525, 571, 753, 989
BseNI ACTGG 1 cut(s) 706
BseXI GCAGC 5 cut(s) 140, 244, 497, 701, 770
BshFI GGCC 2 cut(s) 196, 597
BsiHKAI GWGCWC 2 cut(s) 41, 66
BsiSI CCGG 1 cut(s) 180
BsiWI CGTACG 1 cut(s) 665
BslI CCNNNNNNNGG 2 cut(s) 225, 464
BsmAI GTCTC 1 cut(s) 914
BsmI GAATGC 1 cut(s) 155
BsnI GGCC 2 cut(s) 196, 597
Bso31I GGTCTC 1 cut(s) 914
Bsp1286I GDGCHC 2 cut(s) 41, 66
Bsp143I GATC 4 cut(s) 7, 474, 520, 1009
Bsp1720I GCTNAGC 1 cut(s) 557
BspACI CCGC 3 cut(s) 126, 565, 743
BspANI GGCC 2 cut(s) 196, 597
BspCNI CTCAG 6 cut(s) 79, 346, 526, 570, 752, 988
BspLI GGNNCC 1 cut(s) 433
BspPI GGATC 2 cut(s) 2, 482
BspTNI GGTCTC 1 cut(s) 914
BsrDI GCAATG 2 cut(s) 39, 570
BsrFI RCCGGY 1 cut(s) 179
BsrI ACTGG 1 cut(s) 706
BssAI RCCGGY 1 cut(s) 179
BssECI CCNNGG 2 cut(s) 427, 625
BssMI GATC 4 cut(s) 7, 474, 520, 1009
BssT1I CCWWGG 1 cut(s) 427
Bst2UI CCWGG 1 cut(s) 594
Bst4CI ACNGT 3 cut(s) 493, 967, 1003
Bst6I CTCTTC 1 cut(s) 719
BstBAI YACGTR 1 cut(s) 646
BstC8I GCNNGC 2 cut(s) 198, 563
BstDEI CTNAG 9 cut(s) 87, 210, 333, 534, 557, 739, 887, 975, 1025
BstF5I GGATG 5 cut(s) 98, 735, 751, 875, 988
BstH2I RGCGCY 1 cut(s) 547
BstHHI GCGC 1 cut(s) 546
BstKTI GATC 4 cut(s) 10, 477, 523, 1012
BstMAI GTCTC 1 cut(s) 914
BstMBI GATC 4 cut(s) 7, 474, 520, 1009
BstMWI GCNNNNNNNGC 3 cut(s) 263, 562, 581
BstNI CCWGG 1 cut(s) 594
BstNSI RCATGY 2 cut(s) 515, 1034
BstSCI CCNGG 1 cut(s) 592
BstSFI CTRYAG 1 cut(s) 966
BstV1I GCAGC 5 cut(s) 140, 244, 497, 701, 770
BstX2I RGATCY 2 cut(s) 7, 1009
BstYI RGATCY 2 cut(s) 7, 1009
BsuRI GGCC 2 cut(s) 196, 597
BtsCI GGATG 5 cut(s) 98, 735, 751, 875, 988
BtsI GCAGTG 3 cut(s) 717, 797, 850
BtsIMutI CAGTG 6 cut(s) 240, 717, 797, 850, 963, 1008
Cac8I GCNNGC 2 cut(s) 198, 563
CaiI CAGNNNCTG 1 cut(s) 799
CfoI GCGC 1 cut(s) 546
Cfr10I RCCGGY 1 cut(s) 179
Cfr13I GGNCC 1 cut(s) 622
Csp6I GTAC 4 cut(s) 340, 643, 666, 736
CviAII CATG 5 cut(s) 464, 512, 524, 997, 1031
CviQI GTAC 4 cut(s) 340, 643, 666, 736
DdeI CTNAG 9 cut(s) 87, 210, 333, 534, 557, 739, 887, 975, 1025
DpnI GATC 4 cut(s) 9, 476, 522, 1011
DpnII GATC 4 cut(s) 7, 474, 520, 1009
DriI GACNNNNNGTC 2 cut(s) 183, 501
Eam1104I CTCTTC 1 cut(s) 719
Eam1105I GACNNNNNGTC 2 cut(s) 183, 501
EarI CTCTTC 1 cut(s) 719
Ecl136II GAGCTC 2 cut(s) 39, 64
Eco130I CCWWGG 1 cut(s) 427
Eco147I AGGCCT 1 cut(s) 597
Eco24I GRGCYC 2 cut(s) 41, 66
Eco31I GGTCTC 1 cut(s) 914
Eco32I GATATC 1 cut(s) 961
Eco47I GGWCC 1 cut(s) 622
Eco53kI GAGCTC 2 cut(s) 39, 64
Eco57I CTGAAG 1 cut(s) 130
EcoICRI GAGCTC 2 cut(s) 39, 64
EcoRII CCWGG 1 cut(s) 592
EcoRV GATATC 1 cut(s) 961
EcoT14I CCWWGG 1 cut(s) 427
EcoT38I GRGCYC 2 cut(s) 41, 66
ErhI CCWWGG 1 cut(s) 427
FaeI CATG 5 cut(s) 467, 515, 527, 1000, 1034
FalI AAGNNNNNCTT 2 cut(s) 1018, 1050
FatI CATG 5 cut(s) 463, 511, 523, 996, 1030
Fnu4HI GCNGC 5 cut(s) 129, 258, 486, 690, 784
FokI GGATG 5 cut(s) 85, 742, 758, 882, 995
FriOI GRGCYC 2 cut(s) 41, 66
Fsp4HI GCNGC 5 cut(s) 129, 258, 486, 690, 784
FspBI CTAG 3 cut(s) 222, 350, 498
GlaI GCGC 1 cut(s) 545
GluI GCNGC 5 cut(s) 129, 258, 486, 690, 784
GsuI CTGGAG 1 cut(s) 698
HaeII RGCGCY 1 cut(s) 547
HaeIII GGCC 2 cut(s) 196, 597
HapII CCGG 1 cut(s) 180
HhaI GCGC 1 cut(s) 546
Hin1II CATG 5 cut(s) 467, 515, 527, 1000, 1034
Hin6I GCGC 1 cut(s) 544
HinP1I GCGC 1 cut(s) 544
HindIII AAGCTT 2 cut(s) 264, 902
HinfI GANTC 2 cut(s) 311, 846
HpaII CCGG 1 cut(s) 180
HphI GGTGA 5 cut(s) 106, 309, 447, 505, 904
Hpy166II GTNNAC 2 cut(s) 455, 922
Hpy188III TCNNGA 7 cut(s) 44, 188, 350, 478, 518, 659, 956
Hpy8I GTNNAC 2 cut(s) 455, 922
HpyAV CCTTC 2 cut(s) 419, 664
HpyCH4III ACNGT 3 cut(s) 493, 967, 1003
HpyCH4IV ACGT 2 cut(s) 645, 664
HpyCH4V TGCA 3 cut(s) 357, 635, 733
HpyF10VI GCNNNNNNNGC 3 cut(s) 263, 562, 581
HpyF3I CTNAG 9 cut(s) 87, 210, 333, 534, 557, 739, 887, 975, 1025
HpySE526I ACGT 2 cut(s) 645, 664
Hsp92II CATG 5 cut(s) 467, 515, 527, 1000, 1034
HspAI GCGC 1 cut(s) 544
Kzo9I GATC 4 cut(s) 7, 474, 520, 1009
LmnI GCTCC 1 cut(s) 242
Lsp1109I GCAGC 5 cut(s) 140, 244, 497, 701, 770
LweI GCATC 3 cut(s) 622, 720, 973
MaeI CTAG 3 cut(s) 222, 350, 498
MaeII ACGT 2 cut(s) 645, 664
MaeIII GTNAC 4 cut(s) 92, 133, 282, 493
MalI GATC 4 cut(s) 9, 476, 522, 1011
MboI GATC 4 cut(s) 7, 474, 520, 1009
MboII GAAGA 8 cut(s) 17, 261, 264, 267, 616, 647, 736, 750
MflI RGATCY 2 cut(s) 7, 1009
MhlI GDGCHC 2 cut(s) 41, 66
MluCI AATT 7 cut(s) 109, 252, 291, 401, 807, 946, 989
MlyI GAGTC 1 cut(s) 305
MmeI TCCRAC 3 cut(s) 206, 333, 347
MslI CAYNNNNRTG 2 cut(s) 927, 1001
MspA1I CMGCKG 4 cut(s) 131, 565, 743, 927
MspI CCGG 1 cut(s) 180
MspR9I CCNGG 1 cut(s) 594
Mva1269I GAATGC 1 cut(s) 155
MvaI CCWGG 1 cut(s) 594
MwoI GCNNNNNNNGC 3 cut(s) 263, 562, 581
NdeII GATC 4 cut(s) 7, 474, 520, 1009
NlaIII CATG 5 cut(s) 467, 515, 527, 1000, 1034
NlaIV GGNNCC 1 cut(s) 433
NmeAIII GCCGAG 1 cut(s) 572
NmuCI GTSAC 2 cut(s) 282, 493
NspI RCATGY 2 cut(s) 515, 1034
OliI CACNNNNGTG 1 cut(s) 927
PceI AGGCCT 1 cut(s) 597
PcsI WCGNNNNNNNCGW 1 cut(s) 774
PctI GAATGC 1 cut(s) 155
PfeI GAWTC 1 cut(s) 846
Pfl23II CGTACG 1 cut(s) 665
PflMI CCANNNNNTGG 1 cut(s) 225
PkrI GCNGC 5 cut(s) 130, 259, 487, 691, 785
PleI GAGTC 1 cut(s) 305
PpsI GAGTC 1 cut(s) 305
Ppu21I YACGTR 1 cut(s) 646
Psp124BI GAGCTC 2 cut(s) 41, 66
Psp6I CCWGG 1 cut(s) 592
PspGI CCWGG 1 cut(s) 592
PspLI CGTACG 1 cut(s) 665
PspN4I GGNNCC 1 cut(s) 433
PspPI GGNCC 1 cut(s) 622
PstNI CAGNNNCTG 1 cut(s) 799
PsuI RGATCY 2 cut(s) 7, 1009
PvuII CAGCTG 2 cut(s) 131, 927
RsaI GTAC 4 cut(s) 341, 644, 667, 737
RsaNI GTAC 4 cut(s) 340, 643, 666, 736
RseI CAYNNNNRTG 2 cut(s) 927, 1001
SacI GAGCTC 2 cut(s) 41, 66
SatI GCNGC 5 cut(s) 129, 258, 486, 690, 784
Sau3AI GATC 4 cut(s) 7, 474, 520, 1009
Sau96I GGNCC 1 cut(s) 622
ScaI AGTACT 1 cut(s) 737
SchI GAGTC 1 cut(s) 305
ScrFI CCNGG 1 cut(s) 594
SduI GDGCHC 2 cut(s) 41, 66
SfaNI GCATC 3 cut(s) 622, 720, 973
SfcI CTRYAG 1 cut(s) 966
SinI GGWCC 1 cut(s) 622
SmiMI CAYNNNNRTG 2 cut(s) 927, 1001
SmlI CTYRAG 2 cut(s) 516, 1040
SmoI CTYRAG 2 cut(s) 516, 1040
SpeI ACTAGT 1 cut(s) 221
Sse9I AATT 7 cut(s) 109, 252, 291, 401, 807, 946, 989
SseBI AGGCCT 1 cut(s) 597
SsiI CCGC 3 cut(s) 126, 565, 743
SspI AATATT 1 cut(s) 389
SspMI CTAG 3 cut(s) 222, 350, 498
SstI GAGCTC 2 cut(s) 41, 66
StuI AGGCCT 1 cut(s) 597
StyD4I CCNGG 1 cut(s) 592
StyI CCWWGG 1 cut(s) 427
TaaI ACNGT 3 cut(s) 493, 967, 1003
TaiI ACGT 2 cut(s) 648, 667
TaqI TCGA 1 cut(s) 119
TaqII GACCGA 1 cut(s) 610
TasI AATT 7 cut(s) 109, 252, 291, 401, 807, 946, 989
TatI WGTACW 2 cut(s) 339, 735
TfiI GAWTC 1 cut(s) 846
TscAI CASTG 6 cut(s) 247, 717, 804, 857, 970, 1008
TseFI GTSAC 2 cut(s) 282, 493
TseI GCWGC 5 cut(s) 128, 257, 485, 689, 783
Tsp45I GTSAC 2 cut(s) 282, 493
TspDTI ATGAA 3 cut(s) 148, 559, 750
TspRI CASTG 6 cut(s) 247, 717, 804, 857, 970, 1008
Van91I CCANNNNNTGG 1 cut(s) 225
VpaK11BI GGWCC 1 cut(s) 622
XapI RAATTY 1 cut(s) 109
XbaI TCTAGA 1 cut(s) 349
XceI RCATGY 2 cut(s) 515, 1034
XspI CTAG 3 cut(s) 222, 350, 498
ZrmI AGTACT 1 cut(s) 737
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.