Rroxscaffold_2G00134100

Transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000792
Physical Location & Seq
Forward (+)
71562647 .. 71565070
2424 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00134100.1

Sequence Viewer

Length: 1056 bp
ATGGAGATGATCTCATCAGCAAAATGGGTATCTGAATTGGAAATGGAAGATCCCACTTTCCTGAATCAATATGAAATGAATTCTCATCTGGACTACTCACTTGAAGATCTAAATTTCCAGTCTTTTTCTGCTGAGAGCTACTCATCTTACCCAGATTTTACCCCTCATCAAAATGATGTTGATCAAAGGCCAGCAAAACAGCCCAAGAATAACCACAGTAACTGGAACTCTTGCACTACCACTCATGATCACATAATTACAGTCCCAATGGCTTCCTCTTCTGCCTCCTCACACCTAATTTCTTTTGACAACTCCAACTCATCAGCATATTGTTCTCTTGATTGCACTACTACTGTGAAGCCAAAGAATGAGGTCCTGGGTTCTGGTGAAAAATTGAATTCAATTTCAACTATGATTTCACAAGGTAATTCCTATGACCCCCAAACTTGTTCACCAAACCGTGCATATGGCCAAGGGATCAAGAGGGCTGCTACTGTCACTAGAAGTCCCTTACATGCTCAAGATCACGTTCTAGCTGAGCGAAAGCGCCGAGAAAAGCTCAGCCAGCGGTTCATTGCTCTATCAGCCCTAGTACCAGGTCTAAAGAAGATGGACAAGGCTTCAGTGCTTGGAGATGCCATAAAATATGTGAAACAGCTGCAAGAACGTATGAAGATACTAGAGGAACAAGCGGCAAAGAAAACCGTGGAGTCAGTGGTGTTTGTGAAGAGAACTCAGTACTCTGTGGATGATGATATCTCCTCATCCGATGAGAACTTCGACGGCTGCTCCGATCAACCACTTCCTGAAATCGAAGCTAGGGTTTCAGACAAAGAGGTTCTCATACGAATCCACTGTGAGAAAAAGAAAGGGTGTCTGGCAAACATACTAAGCGAAATAGAGAAGCTTGATCTCACCATTCTTAATAGCAGCGTCTTGCCCTTTGGCAATTCAACTCTTGATATCACTGTCGTTTCTCAGATGGACGTTGAGTACAGCATGACAGTGAAGGAGCTTGTAAGAAAGCTAAGACAAGCTTTGCTCAAGTTGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

351

Amino Acids

39.34

Weight (kDa)

5.77

Isoelectric Point (pI)

47.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 176 - 221 1.1e-10 Helix-loop-helix DNA-binding domain
bHLH-TF_ACT-like_plant PF22754 275 - 346 1.5e-06 Plant bHLH transcription factor, ACT-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 568, 692
AclWI GGATC 2 cut(s) 44, 485
AcoI YGGCCR 1 cut(s) 469
AcsI RAATTY 3 cut(s) 79, 112, 397
AcuI CTGAAG 1 cut(s) 606
AfaI GTAC 3 cut(s) 594, 740, 995
AgsI TTSAA 5 cut(s) 104, 397, 402, 408, 954
AjnI CCWGG 2 cut(s) 375, 595
AluBI AGCT 9 cut(s) 138, 536, 559, 658, 818, 907, 1015, 1027, 1037
AluI AGCT 9 cut(s) 138, 536, 559, 658, 818, 907, 1015, 1027, 1037
AlwI GGATC 2 cut(s) 44, 485
AlwNI CAGNNNCTG 1 cut(s) 222
AoxI GGCC 2 cut(s) 188, 469
ApeKI GCWGC 4 cut(s) 488, 658, 786, 930
ApoI RAATTY 3 cut(s) 79, 112, 397
AspLEI GCGC 1 cut(s) 549
AspS9I GGNCC 1 cut(s) 373
AsuHPI GGTGA 3 cut(s) 398, 444, 907
AvaII GGWCC 1 cut(s) 373
BalI TGGCCA 1 cut(s) 471
BbvI GCAGC 4 cut(s) 475, 645, 773, 942
BccI CCATC 2 cut(s) 604, 976
BceAI ACGGC 1 cut(s) 799
BciT130I CCWGG 2 cut(s) 377, 597
BclI TGATCA 2 cut(s) 181, 247
BfaI CTAG 5 cut(s) 501, 533, 590, 680, 819
BfoI RGCGCY 1 cut(s) 550
BglII AGATCT 1 cut(s) 106
BisI GCNGC 5 cut(s) 489, 659, 693, 787, 931
BlpI GCTNAGC 2 cut(s) 537, 560
BlsI GCNGC 5 cut(s) 490, 660, 694, 788, 932
BmcAI AGTACT 1 cut(s) 740
Bme1390I CCNGG 2 cut(s) 377, 597
Bme18I GGWCC 1 cut(s) 373
BmgT120I GGNCC 1 cut(s) 373
BmrFI CCNGG 2 cut(s) 377, 597
BmsI GCATC 1 cut(s) 625
BplI GAGNNNNNCTC 5 cut(s) 28, 125, 157, 543, 575
Bpu1102I GCTNAGC 2 cut(s) 537, 560
BpuEI CTTGAG 2 cut(s) 504, 1028
BsaBI GATNNNNATC 1 cut(s) 180
BsaJI CCNNGG 3 cut(s) 376, 472, 705
BsaXI ACNNNNNCTCC 2 cut(s) 773, 803
Bse1I ACTGG 2 cut(s) 118, 227
Bse3DI GCAATG 1 cut(s) 573
Bse8I GATNNNNATC 1 cut(s) 180
BseBI CCWGG 2 cut(s) 377, 597
BseDI CCNNGG 3 cut(s) 376, 472, 705
BseGI GGATG 2 cut(s) 754, 764
BseJI GATNNNNATC 1 cut(s) 180
BseMI GCAATG 1 cut(s) 573
BseMII CTCAG 5 cut(s) 123, 528, 574, 749, 992
BseNI ACTGG 2 cut(s) 118, 227
BseRI GAGGAG 2 cut(s) 277, 751
BseXI GCAGC 4 cut(s) 475, 645, 773, 942
BshFI GGCC 2 cut(s) 190, 471
BslFI GGGAC 2 cut(s) 248, 492
BsmFI GGGAC 2 cut(s) 248, 492
BsnI GGCC 2 cut(s) 190, 471
Bsp143I GATC 9 cut(s) 9, 49, 106, 181, 247, 477, 523, 793, 910
Bsp1720I GCTNAGC 2 cut(s) 537, 560
BspACI CCGC 2 cut(s) 568, 692
BspANI GGCC 2 cut(s) 190, 471
BspCNI CTCAG 5 cut(s) 124, 529, 573, 748, 991
BspHI TCATGA 1 cut(s) 244
BspPI GGATC 2 cut(s) 44, 485
BsrDI GCAATG 1 cut(s) 573
BsrI ACTGG 2 cut(s) 118, 227
BssECI CCNNGG 3 cut(s) 376, 472, 705
BssMI GATC 9 cut(s) 9, 49, 106, 181, 247, 477, 523, 793, 910
BssT1I CCWWGG 1 cut(s) 472
Bst2UI CCWGG 2 cut(s) 377, 597
Bst4CI ACNGT 9 cut(s) 218, 262, 355, 461, 496, 706, 857, 970, 1006
Bst6I CTCTTC 2 cut(s) 283, 722
BstC8I GCNNGC 2 cut(s) 192, 566
BstDEI CTNAG 7 cut(s) 132, 537, 560, 735, 890, 978, 1028
BstDSI CCRYGG 1 cut(s) 705
BstF5I GGATG 2 cut(s) 754, 764
BstH2I RGCGCY 1 cut(s) 550
BstHHI GCGC 1 cut(s) 549
BstKTI GATC 9 cut(s) 12, 52, 109, 184, 250, 480, 526, 796, 913
BstMBI GATC 9 cut(s) 9, 49, 106, 181, 247, 477, 523, 793, 910
BstMWI GCNNNNNNNGC 2 cut(s) 565, 584
BstNI CCWGG 2 cut(s) 377, 597
BstNSI RCATGY 1 cut(s) 518
BstSCI CCNGG 2 cut(s) 375, 595
BstV1I GCAGC 4 cut(s) 475, 645, 773, 942
BstX2I RGATCY 2 cut(s) 49, 106
BstYI RGATCY 2 cut(s) 49, 106
BsuRI GGCC 2 cut(s) 190, 471
BtgI CCRYGG 1 cut(s) 705
BtsCI GGATG 2 cut(s) 754, 764
BtsIMutI CAGTG 5 cut(s) 630, 720, 853, 966, 1011
Cac8I GCNNGC 2 cut(s) 192, 566
CaiI CAGNNNCTG 1 cut(s) 222
CciI TCATGA 1 cut(s) 244
CfoI GCGC 1 cut(s) 549
Cfr13I GGNCC 1 cut(s) 373
CseI GACGC 1 cut(s) 922
CsiI ACCWGGT 1 cut(s) 595
Csp6I GTAC 3 cut(s) 593, 739, 994
CviAII CATG 3 cut(s) 245, 515, 1000
CviQI GTAC 3 cut(s) 593, 739, 994
DdeI CTNAG 7 cut(s) 132, 537, 560, 735, 890, 978, 1028
DpnI GATC 9 cut(s) 11, 51, 108, 183, 249, 479, 525, 795, 912
DpnII GATC 9 cut(s) 9, 49, 106, 181, 247, 477, 523, 793, 910
EaeI YGGCCR 1 cut(s) 469
Eam1104I CTCTTC 2 cut(s) 283, 722
EarI CTCTTC 2 cut(s) 283, 722
Eco130I CCWWGG 1 cut(s) 472
Eco32I GATATC 2 cut(s) 757, 964
Eco47I GGWCC 1 cut(s) 373
Eco57I CTGAAG 1 cut(s) 606
EcoO109I RGGNCCY 1 cut(s) 373
EcoRI GAATTC 2 cut(s) 79, 397
EcoRII CCWGG 2 cut(s) 375, 595
EcoRV GATATC 2 cut(s) 757, 964
EcoT14I CCWWGG 1 cut(s) 472
ErhI CCWWGG 1 cut(s) 472
FaeI CATG 3 cut(s) 248, 518, 1003
FalI AAGNNNNNCTT 2 cut(s) 1021, 1053
FaqI GGGAC 2 cut(s) 248, 492
FatI CATG 3 cut(s) 244, 514, 999
FauNDI CATATG 1 cut(s) 466
FbaI TGATCA 2 cut(s) 181, 247
Fnu4HI GCNGC 5 cut(s) 489, 659, 693, 787, 931
FokI GGATG 2 cut(s) 751, 761
Fsp4HI GCNGC 5 cut(s) 489, 659, 693, 787, 931
FspBI CTAG 5 cut(s) 501, 533, 590, 680, 819
GlaI GCGC 1 cut(s) 548
GluI GCNGC 5 cut(s) 489, 659, 693, 787, 931
HaeII RGCGCY 1 cut(s) 550
HaeIII GGCC 2 cut(s) 190, 471
HgaI GACGC 1 cut(s) 922
HhaI GCGC 1 cut(s) 549
Hin1II CATG 3 cut(s) 248, 518, 1003
Hin6I GCGC 1 cut(s) 547
HinP1I GCGC 1 cut(s) 547
HindIII AAGCTT 2 cut(s) 905, 1035
HinfI GANTC 3 cut(s) 64, 710, 849
HphI GGTGA 3 cut(s) 398, 444, 907
Hpy166II GTNNAC 1 cut(s) 452
Hpy188I TCNGA 5 cut(s) 34, 769, 793, 829, 981
Hpy188III TCNNGA 8 cut(s) 61, 89, 245, 338, 481, 521, 806, 959
Hpy8I GTNNAC 1 cut(s) 452
Hpy99I CGWCG 1 cut(s) 785
HpyAV CCTTC 1 cut(s) 1003
HpyCH4III ACNGT 9 cut(s) 218, 262, 355, 461, 496, 706, 857, 970, 1006
HpyCH4IV ACGT 3 cut(s) 528, 667, 987
HpyCH4V TGCA 4 cut(s) 234, 345, 464, 661
HpyF10VI GCNNNNNNNGC 2 cut(s) 565, 584
HpyF3I CTNAG 7 cut(s) 132, 537, 560, 735, 890, 978, 1028
HpySE526I ACGT 3 cut(s) 528, 667, 987
Hsp92II CATG 3 cut(s) 248, 518, 1003
HspAI GCGC 1 cut(s) 547
Ksp22I TGATCA 2 cut(s) 181, 247
Kzo9I GATC 9 cut(s) 9, 49, 106, 181, 247, 477, 523, 793, 910
LmnI GCTCC 2 cut(s) 794, 1012
Lsp1109I GCAGC 4 cut(s) 475, 645, 773, 942
LweI GCATC 1 cut(s) 625
MabI ACCWGGT 1 cut(s) 595
MaeI CTAG 5 cut(s) 501, 533, 590, 680, 819
MaeII ACGT 3 cut(s) 528, 667, 987
MaeIII GTNAC 2 cut(s) 218, 496
MalI GATC 9 cut(s) 11, 51, 108, 183, 249, 479, 525, 795, 912
MboI GATC 9 cut(s) 9, 49, 106, 181, 247, 477, 523, 793, 910
MboII GAAGA 6 cut(s) 59, 116, 270, 619, 685, 739
MflI RGATCY 2 cut(s) 49, 106
MlsI TGGCCA 1 cut(s) 471
MluNI TGGCCA 1 cut(s) 471
MlyI GAGTC 1 cut(s) 719
MmeI TCCRAC 1 cut(s) 339
MnlI CCTC 9 cut(s) 174, 286, 295, 298, 364, 477, 676, 772, 829
Mox20I TGGCCA 1 cut(s) 471
MscI TGGCCA 1 cut(s) 471
MseI TTAA 1 cut(s) 924
MslI CAYNNNNRTG 2 cut(s) 171, 1004
Msp20I TGGCCA 1 cut(s) 471
MspA1I CMGCKG 2 cut(s) 568, 658
MspR9I CCNGG 2 cut(s) 377, 597
MvaI CCWGG 2 cut(s) 377, 597
MwoI GCNNNNNNNGC 2 cut(s) 565, 584
NdeI CATATG 1 cut(s) 466
NdeII GATC 9 cut(s) 9, 49, 106, 181, 247, 477, 523, 793, 910
NlaIII CATG 3 cut(s) 248, 518, 1003
NmeAIII GCCGAG 1 cut(s) 575
NmuCI GTSAC 1 cut(s) 496
NspI RCATGY 1 cut(s) 518
PagI TCATGA 1 cut(s) 244
PcsI WCGNNNNNNNCGW 1 cut(s) 789
PfeI GAWTC 2 cut(s) 64, 849
PkrI GCNGC 5 cut(s) 490, 660, 694, 788, 932
PleI GAGTC 1 cut(s) 718
PpsI GAGTC 1 cut(s) 718
PpuMI RGGWCCY 1 cut(s) 373
Psp5II RGGWCCY 1 cut(s) 373
Psp6I CCWGG 2 cut(s) 375, 595
PspGI CCWGG 2 cut(s) 375, 595
PspPI GGNCC 1 cut(s) 373
PspPPI RGGWCCY 1 cut(s) 373
PstNI CAGNNNCTG 1 cut(s) 222
PsuI RGATCY 2 cut(s) 49, 106
PvuII CAGCTG 1 cut(s) 658
RsaI GTAC 3 cut(s) 594, 740, 995
RsaNI GTAC 3 cut(s) 593, 739, 994
RseI CAYNNNNRTG 2 cut(s) 171, 1004
SaqAI TTAA 1 cut(s) 924
SatI GCNGC 5 cut(s) 489, 659, 693, 787, 931
Sau3AI GATC 9 cut(s) 9, 49, 106, 181, 247, 477, 523, 793, 910
Sau96I GGNCC 1 cut(s) 373
ScaI AGTACT 1 cut(s) 740
SchI GAGTC 1 cut(s) 719
ScrFI CCNGG 2 cut(s) 377, 597
SexAI ACCWGGT 1 cut(s) 595
SfaNI GCATC 1 cut(s) 625
SinI GGWCC 1 cut(s) 373
SmiMI CAYNNNNRTG 2 cut(s) 171, 1004
SmlI CTYRAG 2 cut(s) 519, 1043
SmoI CTYRAG 2 cut(s) 519, 1043
SsiI CCGC 2 cut(s) 568, 692
SspMI CTAG 5 cut(s) 501, 533, 590, 680, 819
StyD4I CCNGG 2 cut(s) 375, 595
StyI CCWWGG 1 cut(s) 472
TaaI ACNGT 9 cut(s) 218, 262, 355, 461, 496, 706, 857, 970, 1006
TaiI ACGT 3 cut(s) 531, 670, 990
TaqI TCGA 2 cut(s) 780, 813
TatI WGTACW 2 cut(s) 738, 993
TauI GCSGC 1 cut(s) 695
TfiI GAWTC 2 cut(s) 64, 849
Tru1I TTAA 1 cut(s) 924
Tru9I TTAA 1 cut(s) 924
TscAI CASTG 5 cut(s) 630, 720, 860, 973, 1011
TseFI GTSAC 1 cut(s) 496
TseI GCWGC 4 cut(s) 488, 658, 786, 930
Tsp45I GTSAC 1 cut(s) 496
TspDTI ATGAA 4 cut(s) 87, 92, 562, 686
TspRI CASTG 5 cut(s) 630, 720, 860, 973, 1011
VpaK11BI GGWCC 1 cut(s) 373
XapI RAATTY 3 cut(s) 79, 112, 397
XceI RCATGY 1 cut(s) 518
XspI CTAG 5 cut(s) 501, 533, 590, 680, 819
ZrmI AGTACT 1 cut(s) 740
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.