MD15G1305200.v1.1

Transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000792
Physical Location & Seq
Reverse (-)
29929181 .. 29930577
1397 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1305200.v1.1.491

Sequence Viewer

Length: 1026 bp
ATGAGCTCCCGGGACTACTCACTTGATGAGCTCAATTTCCTATCTTTCTCATCTGAGAGCTACTCATCCTATCCAAATTTCACCCCAAAAGCCGACAACTTCAGCAAAGCATCCATCGAAAATCTTCATCAGATGTACGGGACTCATGAAAGGCCAGCAAAACAGCCTAAGAACAACACTAATAGTTGGAACTCTTGCTCCACTGACCACATAATTACTGCCAATGCTTCTTCTTCCTCCTCTTCACACCTAATTTCTTTTGAAAGCTCCAACTCATCACCCCCAACCACTTCTCAGCAGTACTATGGTCTAGATTGCAGAGTTATCAACCCAAAGAATGAAGTGGAATATTCCAATGGGAAATTGAACCCTCGAGCTTTGATTTCCCAAGGTATCTATGACCCCCAAACATGCTCACCGAAACATGGACAGGGGATCAAGAGGGCGGCTACGGTGACTAAAAGTCCTTTAGATGCTCAAGATCATGTTCTTGCTGAGAGAAAGCGCCGAGAAAATCTCAGCCAGCGGTTCATTGCTCTATCTGCCTTACTTCCAGGCCTGAAGAAGACGGACAAGGCTTCGGTCCTCGGAGATGCGATCAAGTACGTGAAACATCTTCAAGAACTTACGAAAATGCTGGAGAAACAAGCAGCCAAGAAAACTGTGGAAGCGGTGGTGTTTGTGAAGAGGACTCAGTACTCAGCGGATGATGATATATCCTCATCCGATGAGAACTCCGAGAGCTGCTCTAACCAGCCACTACCAGAAATCGAAGCAAGAGTTTCGGACAAGGAGGTCCTCATACGAATCCACAGCGAGAAAACTAAAGGATCTTTGGCAAGCATACTGAGTGAAATAGAAAAGCTTGATCTCACCATTGTTCACAGCTGTGCCTTGCCCCTTGGCAATTCAACTCTAGATATCACTGTAGTTGCTCAGATGGATGTAGAATTCAGAATGACAGTGAAAGATCTTGTAAAAAATCTAAGACAGGCTTTGCTCAAGTTGGTGGGACCAGAAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

342

Amino Acids

37.78

Weight (kDa)

7.12

Isoelectric Point (pI)

48.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 162 - 207 6.5e-11 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 794
AciI CCGC 4 cut(s) 446, 526, 671, 704
AclWI GGATC 2 cut(s) 443, 838
AcsI RAATTY 2 cut(s) 76, 950
AcuI CTGAAG 2 cut(s) 85, 581
AfaI GTAC 4 cut(s) 137, 302, 605, 698
AfiI CCNNNNNNNGG 1 cut(s) 425
AgsI TTSAA 4 cut(s) 263, 367, 620, 912
AhdI GACNNNNNGTC 1 cut(s) 462
AjnI CCWGG 1 cut(s) 553
AleI CACNNNNGTG 1 cut(s) 888
AloI GAACNNNNNNTCC 2 cut(s) 182, 214
AluBI AGCT 8 cut(s) 6, 31, 60, 267, 377, 744, 865, 888
AluI AGCT 8 cut(s) 6, 31, 60, 267, 377, 744, 865, 888
Alw21I GWGCWC 2 cut(s) 8, 33
AlwI GGATC 2 cut(s) 443, 838
Ama87I CYCGRG 2 cut(s) 9, 372
AoxI GGCC 2 cut(s) 152, 556
ApeKI GCWGC 2 cut(s) 650, 744
ApoI RAATTY 2 cut(s) 76, 950
Asp700I GAANNNNTTC 1 cut(s) 123
AspLEI GCGC 1 cut(s) 507
AspS9I GGNCC 3 cut(s) 583, 796, 1013
AsuC2I CCSGG 2 cut(s) 10, 11
AsuHPI GGTGA 5 cut(s) 73, 270, 408, 466, 865
AvaI CYCGRG 2 cut(s) 9, 372
AvaII GGWCC 3 cut(s) 583, 796, 1013
BanII GRGCYC 2 cut(s) 8, 33
BbsI GAAGAC 1 cut(s) 572
Bbv12I GWGCWC 2 cut(s) 8, 33
BbvI GCAGC 2 cut(s) 662, 731
BccI CCATC 2 cut(s) 122, 934
BcgI CGANNNNNNTGC 2 cut(s) 765, 799
BciT130I CCWGG 1 cut(s) 555
BcnI CCSGG 2 cut(s) 10, 11
BfaI CTAG 2 cut(s) 311, 917
BfmI CTRYAG 1 cut(s) 927
BfoI RGCGCY 1 cut(s) 508
BglII AGATCT 1 cut(s) 970
BisI GCNGC 3 cut(s) 447, 651, 745
BlsI GCNGC 3 cut(s) 448, 652, 746
BmcAI AGTACT 2 cut(s) 302, 698
Bme1390I CCNGG 3 cut(s) 10, 11, 555
Bme18I GGWCC 3 cut(s) 583, 796, 1013
BmeRI GACNNNNNGTC 1 cut(s) 462
BmeT110I CYCGRG 2 cut(s) 9, 372
BmgT120I GGNCC 3 cut(s) 583, 796, 1013
BmiI GGNNCC 1 cut(s) 1014
BmrFI CCNGG 3 cut(s) 10, 11, 555
BmsI GCATC 3 cut(s) 119, 463, 583
BpiI GAAGAC 1 cut(s) 572
BplI GAGNNNNNCTC 6 cut(s) 47, 79, 501, 533, 731, 763
BpmI CTGGAG 1 cut(s) 659
BpuEI CTTGAG 2 cut(s) 462, 986
BpuMI CCSGG 2 cut(s) 10, 11
BsaAI YACGTR 1 cut(s) 607
BsaJI CCNNGG 4 cut(s) 9, 388, 586, 901
BsaXI ACNNNNNCTCC 2 cut(s) 182, 212
Bsc4I CCNNNNNNNGG 1 cut(s) 425
Bse3DI GCAATG 1 cut(s) 531
BseBI CCWGG 1 cut(s) 555
BseDI CCNNGG 4 cut(s) 9, 388, 586, 901
BseGI GGATG 5 cut(s) 65, 110, 712, 722, 949
BseLI CCNNNNNNNGG 1 cut(s) 425
BseMI GCAATG 1 cut(s) 531
BseMII CTCAG 8 cut(s) 45, 308, 486, 532, 707, 714, 839, 950
BseRI GAGGAG 1 cut(s) 229
BseXI GCAGC 2 cut(s) 662, 731
BshFI GGCC 2 cut(s) 154, 558
BsiHKAI GWGCWC 2 cut(s) 8, 33
BsiHKCI CYCGRG 2 cut(s) 9, 372
BsiSI CCGG 1 cut(s) 10
BslFI GGGAC 2 cut(s) 26, 154
BslI CCNNNNNNNGG 1 cut(s) 425
BsmFI GGGAC 2 cut(s) 26, 154
BsnI GGCC 2 cut(s) 154, 558
BsoBI CYCGRG 2 cut(s) 9, 372
Bsp1286I GDGCHC 2 cut(s) 8, 33
Bsp143I GATC 6 cut(s) 435, 481, 597, 830, 868, 970
BspACI CCGC 4 cut(s) 446, 526, 671, 704
BspANI GGCC 2 cut(s) 154, 558
BspCNI CTCAG 8 cut(s) 46, 307, 487, 531, 706, 713, 840, 949
BspHI TCATGA 1 cut(s) 145
BspLI GGNNCC 1 cut(s) 1014
BspPI GGATC 2 cut(s) 443, 838
BsrDI GCAATG 1 cut(s) 531
BssECI CCNNGG 4 cut(s) 9, 388, 586, 901
BssMI GATC 6 cut(s) 435, 481, 597, 830, 868, 970
BssT1I CCWWGG 2 cut(s) 388, 901
Bst2UI CCWGG 1 cut(s) 555
Bst4CI ACNGT 4 cut(s) 454, 664, 928, 964
Bst6I CTCTTC 2 cut(s) 247, 680
BstBAI YACGTR 1 cut(s) 607
BstC8I GCNNGC 3 cut(s) 156, 524, 841
BstF5I GGATG 5 cut(s) 65, 110, 712, 722, 949
BstH2I RGCGCY 1 cut(s) 508
BstHHI GCGC 1 cut(s) 507
BstKTI GATC 6 cut(s) 438, 484, 600, 833, 871, 973
BstMBI GATC 6 cut(s) 435, 481, 597, 830, 868, 970
BstMWI GCNNNNNNNGC 1 cut(s) 542
BstNI CCWGG 1 cut(s) 555
BstNSI RCATGY 1 cut(s) 414
BstSCI CCNGG 3 cut(s) 8, 9, 553
BstSFI CTRYAG 1 cut(s) 927
BstV1I GCAGC 2 cut(s) 662, 731
BstV2I GAAGAC 1 cut(s) 572
BstX2I RGATCY 2 cut(s) 830, 970
BstYI RGATCY 2 cut(s) 830, 970
BsuRI GGCC 2 cut(s) 154, 558
BtsCI GGATG 5 cut(s) 65, 110, 712, 722, 949
BtsIMutI CAGTG 3 cut(s) 201, 924, 969
Cac8I GCNNGC 3 cut(s) 156, 524, 841
CciI TCATGA 1 cut(s) 145
CfoI GCGC 1 cut(s) 507
Cfr13I GGNCC 3 cut(s) 583, 796, 1013
Cfr9I CCCGGG 1 cut(s) 9
Csp6I GTAC 4 cut(s) 136, 301, 604, 697
CviAII CATG 4 cut(s) 146, 411, 425, 485
CviQI GTAC 4 cut(s) 136, 301, 604, 697
DpnI GATC 6 cut(s) 437, 483, 599, 832, 870, 972
DpnII GATC 6 cut(s) 435, 481, 597, 830, 868, 970
DrdI GACNNNNNNGTC 1 cut(s) 794
DriI GACNNNNNGTC 1 cut(s) 462
DseDI GACNNNNNNGTC 1 cut(s) 794
Eam1104I CTCTTC 2 cut(s) 247, 680
Eam1105I GACNNNNNGTC 1 cut(s) 462
EarI CTCTTC 2 cut(s) 247, 680
Ecl136II GAGCTC 2 cut(s) 6, 31
Eco130I CCWWGG 2 cut(s) 388, 901
Eco147I AGGCCT 1 cut(s) 558
Eco24I GRGCYC 2 cut(s) 8, 33
Eco32I GATATC 1 cut(s) 922
Eco47I GGWCC 3 cut(s) 583, 796, 1013
Eco53kI GAGCTC 2 cut(s) 6, 31
Eco57I CTGAAG 2 cut(s) 85, 581
Eco88I CYCGRG 2 cut(s) 9, 372
EcoICRI GAGCTC 2 cut(s) 6, 31
EcoO109I RGGNCCY 1 cut(s) 796
EcoRI GAATTC 1 cut(s) 950
EcoRII CCWGG 1 cut(s) 553
EcoRV GATATC 1 cut(s) 922
EcoT14I CCWWGG 2 cut(s) 388, 901
EcoT38I GRGCYC 2 cut(s) 8, 33
ErhI CCWWGG 2 cut(s) 388, 901
FaeI CATG 4 cut(s) 149, 414, 428, 488
FalI AAGNNNNNCTT 2 cut(s) 979, 1011
FaqI GGGAC 2 cut(s) 26, 154
FatI CATG 4 cut(s) 145, 410, 424, 484
Fnu4HI GCNGC 3 cut(s) 447, 651, 745
FokI GGATG 5 cut(s) 52, 97, 709, 719, 956
FriOI GRGCYC 2 cut(s) 8, 33
Fsp4HI GCNGC 3 cut(s) 447, 651, 745
FspBI CTAG 2 cut(s) 311, 917
GlaI GCGC 1 cut(s) 506
GluI GCNGC 3 cut(s) 447, 651, 745
GsuI CTGGAG 1 cut(s) 659
HaeII RGCGCY 1 cut(s) 508
HaeIII GGCC 2 cut(s) 154, 558
HapII CCGG 1 cut(s) 10
HhaI GCGC 1 cut(s) 507
Hin1II CATG 4 cut(s) 149, 414, 428, 488
Hin6I GCGC 1 cut(s) 505
HinP1I GCGC 1 cut(s) 505
HindIII AAGCTT 1 cut(s) 863
HinfI GANTC 3 cut(s) 142, 691, 807
HpaII CCGG 1 cut(s) 10
HphI GGTGA 5 cut(s) 73, 270, 408, 466, 865
Hpy166II GTNNAC 1 cut(s) 883
Hpy188I TCNGA 8 cut(s) 55, 132, 590, 727, 739, 787, 939, 956
Hpy188III TCNNGA 6 cut(s) 146, 311, 439, 479, 620, 917
Hpy8I GTNNAC 1 cut(s) 883
HpyCH4III ACNGT 4 cut(s) 454, 664, 928, 964
HpyCH4IV ACGT 1 cut(s) 606
HpyCH4V TGCA 1 cut(s) 318
HpyF10VI GCNNNNNNNGC 1 cut(s) 542
HpySE526I ACGT 1 cut(s) 606
Hsp92II CATG 4 cut(s) 149, 414, 428, 488
HspAI GCGC 1 cut(s) 505
Kzo9I GATC 6 cut(s) 435, 481, 597, 830, 868, 970
LmnI GCTCC 3 cut(s) 11, 203, 272
Lsp1109I GCAGC 2 cut(s) 662, 731
LweI GCATC 3 cut(s) 119, 463, 583
MaeI CTAG 2 cut(s) 311, 917
MaeII ACGT 1 cut(s) 606
MaeIII GTNAC 1 cut(s) 454
MalI GATC 6 cut(s) 437, 483, 599, 832, 870, 972
MboI GATC 6 cut(s) 435, 481, 597, 830, 868, 970
MboII GAAGA 8 cut(s) 116, 222, 225, 234, 574, 577, 608, 697
MflI RGATCY 2 cut(s) 830, 970
MhlI GDGCHC 2 cut(s) 8, 33
MluCI AATT 7 cut(s) 34, 76, 213, 252, 362, 907, 950
MlyI GAGTC 2 cut(s) 136, 685
MmeI TCCRAC 2 cut(s) 167, 294
MnlI CCTC 9 cut(s) 247, 250, 381, 435, 596, 681, 730, 787, 809
MroXI GAANNNNTTC 1 cut(s) 123
MslI CAYNNNNRTG 1 cut(s) 888
MspA1I CMGCKG 3 cut(s) 526, 704, 888
MspI CCGG 1 cut(s) 10
MspR9I CCNGG 3 cut(s) 10, 11, 555
MvaI CCWGG 1 cut(s) 555
MwoI GCNNNNNNNGC 1 cut(s) 542
NciI CCSGG 2 cut(s) 10, 11
NdeII GATC 6 cut(s) 435, 481, 597, 830, 868, 970
NlaIII CATG 4 cut(s) 149, 414, 428, 488
NlaIV GGNNCC 1 cut(s) 1014
NmeAIII GCCGAG 1 cut(s) 533
NmuCI GTSAC 1 cut(s) 454
NspI RCATGY 1 cut(s) 414
OliI CACNNNNGTG 1 cut(s) 888
PaeR7I CTCGAG 1 cut(s) 372
PagI TCATGA 1 cut(s) 145
PceI AGGCCT 1 cut(s) 558
PdmI GAANNNNTTC 1 cut(s) 123
PfeI GAWTC 1 cut(s) 807
PkrI GCNGC 3 cut(s) 448, 652, 746
PleI GAGTC 2 cut(s) 136, 685
PpsI GAGTC 2 cut(s) 136, 685
Ppu21I YACGTR 1 cut(s) 607
PpuMI RGGWCCY 1 cut(s) 796
Psp124BI GAGCTC 2 cut(s) 8, 33
Psp5II RGGWCCY 1 cut(s) 796
Psp6I CCWGG 1 cut(s) 553
PspGI CCWGG 1 cut(s) 553
PspN4I GGNNCC 1 cut(s) 1014
PspPI GGNCC 3 cut(s) 583, 796, 1013
PspPPI RGGWCCY 1 cut(s) 796
PspXI VCTCGAGB 1 cut(s) 372
PsuI RGATCY 2 cut(s) 830, 970
PvuII CAGCTG 1 cut(s) 888
RsaI GTAC 4 cut(s) 137, 302, 605, 698
RsaNI GTAC 4 cut(s) 136, 301, 604, 697
RseI CAYNNNNRTG 1 cut(s) 888
SacI GAGCTC 2 cut(s) 8, 33
SatI GCNGC 3 cut(s) 447, 651, 745
Sau3AI GATC 6 cut(s) 435, 481, 597, 830, 868, 970
Sau96I GGNCC 3 cut(s) 583, 796, 1013
ScaI AGTACT 2 cut(s) 302, 698
SchI GAGTC 2 cut(s) 136, 685
ScrFI CCNGG 3 cut(s) 10, 11, 555
SduI GDGCHC 2 cut(s) 8, 33
SfaNI GCATC 3 cut(s) 119, 463, 583
SfcI CTRYAG 1 cut(s) 927
Sfr274I CTCGAG 1 cut(s) 372
SinI GGWCC 3 cut(s) 583, 796, 1013
SlaI CTCGAG 1 cut(s) 372
SmaI CCCGGG 1 cut(s) 11
SmiMI CAYNNNNRTG 1 cut(s) 888
SmlI CTYRAG 3 cut(s) 372, 477, 1001
SmoI CTYRAG 3 cut(s) 372, 477, 1001
Sse9I AATT 7 cut(s) 34, 76, 213, 252, 362, 907, 950
SseBI AGGCCT 1 cut(s) 558
SsiI CCGC 4 cut(s) 446, 526, 671, 704
SspI AATATT 1 cut(s) 350
SspMI CTAG 2 cut(s) 311, 917
SstI GAGCTC 2 cut(s) 8, 33
StuI AGGCCT 1 cut(s) 558
StyD4I CCNGG 3 cut(s) 8, 9, 553
StyI CCWWGG 2 cut(s) 388, 901
TaaI ACNGT 4 cut(s) 454, 664, 928, 964
TaiI ACGT 1 cut(s) 609
TaqI TCGA 3 cut(s) 117, 373, 771
TaqII GACCGA 1 cut(s) 571
TasI AATT 7 cut(s) 34, 76, 213, 252, 362, 907, 950
TatI WGTACW 2 cut(s) 300, 696
TauI GCSGC 1 cut(s) 449
TfiI GAWTC 1 cut(s) 807
TscAI CASTG 3 cut(s) 208, 931, 969
TseFI GTSAC 1 cut(s) 454
TseI GCWGC 2 cut(s) 650, 744
Tsp45I GTSAC 1 cut(s) 454
TspDTI ATGAA 4 cut(s) 116, 162, 354, 520
TspGWI ACGGA 1 cut(s) 584
TspMI CCCGGG 1 cut(s) 9
TspRI CASTG 3 cut(s) 208, 931, 969
VpaK11BI GGWCC 3 cut(s) 583, 796, 1013
XapI RAATTY 2 cut(s) 76, 950
XbaI TCTAGA 2 cut(s) 310, 916
XceI RCATGY 1 cut(s) 414
XcmI CCANNNNNNNNNTGG 1 cut(s) 661
XhoI CTCGAG 1 cut(s) 372
XmaI CCCGGG 1 cut(s) 9
XmnI GAANNNNTTC 1 cut(s) 123
XspI CTAG 2 cut(s) 311, 917
ZrmI AGTACT 2 cut(s) 302, 698
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.