MD03G1241800.v1.1

EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0001544
Physical Location & Seq
Forward (+)
32674028 .. 32677853
3826 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1241800.v1.1.491

Sequence Viewer

Length: 1434 bp
ATGGCTTCCTTCTCTACCTTGAACCTTTCACCAAGTCCAAAACCCACCCATTCTCTTCTAAAACCACTGATTCCGGCACCCGAAATCGATACCCGAACCCAAATCCAAACCCCTTACCCGAAGCTCATACTCACGGGCCCTTCCCAGCTTTCCGGCCATGTACCCATCAGCGGCTCCAAGAACTCAGCTTTGCCGATTCTGGCGGCGACCCTTTGCTGCTCAGGCACTTCAAGGATTCAAAACGTGCCGGATTTGTTCGATACTCGCACAATGGCTTCGGTTTTAGTGTCTCTTGGAGCTAAAGTCGAAGTCTTGGACAACGAAATGTTGGTTAACACCGATGGGGTTGGCTCAGTTGAGCCTAATGCGGATGAAATTCAAAAGATTAGAGGTGGGTTTTTCGTCATTGGGCCGTTGGTTGCCCGATTCGGGGAGGCGGTGGTTGCATTACCCGGTGGCTGCAATATTGGGGCTCGACCAGTGGACCTATACATTCGGGGTCTTCAATCTCTTGGCGCTGTTGTTGAATTGAGGGATGGGAAAGTTCAGGCATATGCCGCAAATGGAAGAGGATTAGTTGGAGGAAGTTTCCAACTTGATTACCCGAGTGTGGGGGCAACGGAAACTCTTATGATGGCTGCATGTATGGCTGATGGAACAACTGTACTGTCCAATGTTGCCAGAGAACCAGAGGTGGTTGATCTTGCTAGGTTTCTTACCAACTGTGGGGCCTGTGTGGATGGAGCAGGCAGCAACAAGCTTGTTATCAAAGGAAAATCCCAGTTGCGTGGTTGTGAATGCGTTGTTGCACCTGATCGTATTGAAGCAGGTACATTTATGCTTGCTGCAGCAATTACTCGCTCATGCATCTCAATATCACCTGTCATACCTTGCCAAATGTCCTCTTTGATACACAAACTATCGGCCGCTGGTTGCAAAATCAAGAAATGTTCTCATGATACCTTGGAAGTTTCAGCAGTGTCTGGGCGTGGGGGTGAAAATTTGCTTAGCGTCGTGGTTAAGACAGGCCCATATCCTGGGTTTCCCACAGACCTCCAACCACAAATAATGGCTTTGCTGACTACATGCAATGGTTTAAGTTCTGTAGAGGAATCTGTATTTGACAAACGCATGAGTCATGTAAGCGAACTGCTAAAGCTTGGAGCAAAGATTCAGGTCTGTGCGAGCACTGCTCTGGTTTATGGGAAAGACAATGGAAGTGTCTTGAATGGTTCCTCGCTTGTTGCAAATGACATTAGGGGTGGGGTGTCACTGGTATTAGCTGGGTTGGCCGCAGAAGGCACTACAGAGATCAGCGGTGTTGCTCATATTGACCGTGGTTATGAGAATTTAGATATGAAACTTCGTTTTCTGGGAGCTGATGAAATCGAAGAGGCATTGGAGCGCAGTGTGAGCATTCTTGCTCTTCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPSP_synthase

Protein Analysis

478

Amino Acids

49.77

Weight (kDa)

5.88

Isoelectric Point (pI)

35.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPSP_synthase PF00275 45 - 452 2.6e-91 EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 818
AccB1I GGYRCC 1 cut(s) 76
AccB7I CCANNNNNTGG 1 cut(s) 1037
AciI CCGC 8 cut(s) 171, 203, 368, 437, 558, 927, 1293, 1317
AcoI YGGCCR 3 cut(s) 154, 924, 1290
AcsI RAATTY 3 cut(s) 375, 1000, 1348
AfaI GTAC 3 cut(s) 162, 666, 832
AfiI CCNNNNNNNGG 7 cut(s) 170, 429, 430, 610, 611, 726, 1037
AgsI TTSAA 8 cut(s) 22, 231, 239, 380, 506, 527, 824, 1228
AjnI CCWGG 1 cut(s) 1036
AluBI AGCT 8 cut(s) 124, 148, 188, 299, 760, 1159, 1283, 1379
AluI AGCT 8 cut(s) 124, 148, 188, 299, 760, 1159, 1283, 1379
Alw21I GWGCWC 1 cut(s) 1190
Alw26I GTCTC 1 cut(s) 294
AlwNI CAGNNNCTG 1 cut(s) 983
Ama87I CYCGRG 1 cut(s) 604
AoxI GGCC 7 cut(s) 136, 154, 410, 729, 924, 1027, 1290
ApaI GGGCCC 1 cut(s) 140
ApeKI GCWGC 6 cut(s) 216, 459, 638, 750, 845, 848
ApoI RAATTY 3 cut(s) 375, 1000, 1348
AspLEI GCGC 2 cut(s) 518, 1407
AspS9I GGNCC 6 cut(s) 136, 137, 410, 484, 729, 1028
AsuC2I CCSGG 1 cut(s) 453
AsuHPI GGTGA 3 cut(s) 21, 870, 1007
AvaI CYCGRG 1 cut(s) 604
AvaII GGWCC 1 cut(s) 484
BaeGI GKGCMC 1 cut(s) 140
BanI GGYRCC 1 cut(s) 76
BanII GRGCYC 2 cut(s) 140, 475
BbsI GAAGAC 1 cut(s) 494
Bbv12I GWGCWC 1 cut(s) 1190
BbvI GCAGC 6 cut(s) 203, 446, 625, 762, 832, 860
BccI CCATC 6 cut(s) 173, 335, 530, 628, 647, 734
BceAI ACGGC 1 cut(s) 397
BciT130I CCWGG 1 cut(s) 1038
BcnI CCSGG 1 cut(s) 453
BcoDI GTCTC 1 cut(s) 294
BfaI CTAG 1 cut(s) 708
BfmI CTRYAG 3 cut(s) 846, 1104, 1305
BfoI RGCGCY 1 cut(s) 519
BfuAI ACCTGC 1 cut(s) 818
BlpI GCTNAGC 1 cut(s) 1007
Bme1390I CCNGG 2 cut(s) 453, 1038
Bme18I GGWCC 1 cut(s) 484
BmeT110I CYCGRG 1 cut(s) 604
BmgT120I GGNCC 6 cut(s) 136, 137, 410, 484, 729, 1028
BmiI GGNNCC 5 cut(s) 78, 138, 175, 730, 1234
BmrFI CCNGG 2 cut(s) 453, 1038
BmrI ACTGGG 1 cut(s) 775
BmsI GCATC 1 cut(s) 876
BmuI ACTGGG 1 cut(s) 775
BpiI GAAGAC 1 cut(s) 494
BplI GAGNNNNNCTC 2 cut(s) 1177, 1209
Bpu10I CCTNAGC 1 cut(s) 220
Bpu1102I GCTNAGC 1 cut(s) 1007
BpuMI CCSGG 1 cut(s) 453
Bsa29I ATCGAT 1 cut(s) 87
BsaJI CCNNGG 3 cut(s) 963, 1037, 1336
BsaXI ACNNNNNCTCC 2 cut(s) 288, 318
Bsc4I CCNNNNNNNGG 7 cut(s) 170, 429, 430, 610, 611, 726, 1037
Bse1I ACTGG 3 cut(s) 479, 781, 1278
Bse3DI GCAATG 1 cut(s) 1096
BseBI CCWGG 1 cut(s) 1038
BseCI ATCGAT 1 cut(s) 87
BseDI CCNNGG 3 cut(s) 963, 1037, 1336
BseGI GGATG 3 cut(s) 376, 541, 745
BseLI CCNNNNNNNGG 7 cut(s) 170, 429, 430, 610, 611, 726, 1037
BseMI GCAATG 1 cut(s) 1096
BseMII CTCAG 3 cut(s) 198, 234, 366
BseNI ACTGG 3 cut(s) 479, 781, 1278
BseSI GKGCMC 1 cut(s) 140
BseX3I CGGCCG 1 cut(s) 924
BseXI GCAGC 6 cut(s) 203, 446, 625, 762, 832, 860
BseYI CCCAGC 2 cut(s) 144, 1283
Bsh1285I CGRYCG 1 cut(s) 927
BshFI GGCC 7 cut(s) 138, 156, 412, 731, 926, 1029, 1292
BshNI GGYRCC 1 cut(s) 76
BshVI ATCGAT 1 cut(s) 87
BsiEI CGRYCG 1 cut(s) 927
BsiHKAI GWGCWC 1 cut(s) 1190
BsiHKCI CYCGRG 1 cut(s) 604
BsiSI CCGG 4 cut(s) 74, 153, 248, 453
BslI CCNNNNNNNGG 7 cut(s) 170, 429, 430, 610, 611, 726, 1037
BsmAI GTCTC 1 cut(s) 294
BsmI GAATGC 2 cut(s) 803, 1416
BsnI GGCC 7 cut(s) 138, 156, 412, 731, 926, 1029, 1292
BsoBI CYCGRG 1 cut(s) 604
Bsp120I GGGCCC 1 cut(s) 136
Bsp1286I GDGCHC 3 cut(s) 140, 475, 1190
Bsp143I GATC 3 cut(s) 700, 814, 1311
Bsp1720I GCTNAGC 1 cut(s) 1007
BspACI CCGC 8 cut(s) 171, 203, 368, 437, 558, 927, 1293, 1317
BspANI GGCC 7 cut(s) 138, 156, 412, 731, 926, 1029, 1292
BspCNI CTCAG 3 cut(s) 197, 233, 365
BspDI ATCGAT 1 cut(s) 87
BspHI TCATGA 1 cut(s) 955
BspLI GGNNCC 5 cut(s) 78, 138, 175, 730, 1234
BspMAI CTGCAG 1 cut(s) 850
BspMI ACCTGC 1 cut(s) 818
BspQI GCTCTTC 1 cut(s) 1431
BspT107I GGYRCC 1 cut(s) 76
BsrDI GCAATG 1 cut(s) 1096
BsrI ACTGG 3 cut(s) 479, 781, 1278
BssECI CCNNGG 3 cut(s) 963, 1037, 1336
BssMI GATC 3 cut(s) 700, 814, 1311
BssT1I CCWWGG 1 cut(s) 963
Bst2UI CCWGG 1 cut(s) 1038
Bst4CI ACNGT 4 cut(s) 664, 669, 725, 1337
Bst6I CTCTTC 4 cut(s) 60, 562, 1386, 1431
BstC8I GCNNGC 3 cut(s) 748, 843, 1186
BstDEI CTNAG 4 cut(s) 184, 220, 352, 1007
BstDSI CCRYGG 1 cut(s) 1336
BstF5I GGATG 3 cut(s) 376, 541, 745
BstH2I RGCGCY 1 cut(s) 519
BstHHI GCGC 2 cut(s) 518, 1407
BstKTI GATC 3 cut(s) 703, 817, 1314
BstMAI GTCTC 1 cut(s) 294
BstMBI GATC 3 cut(s) 700, 814, 1311
BstMCI CGRYCG 1 cut(s) 927
BstMWI GCNNNNNNNGC 7 cut(s) 222, 443, 557, 647, 1190, 1289, 1413
BstNI CCWGG 1 cut(s) 1038
BstNSI RCATGY 2 cut(s) 645, 1089
BstSCI CCNGG 2 cut(s) 451, 1036
BstSFI CTRYAG 3 cut(s) 846, 1104, 1305
BstSLI GKGCMC 1 cut(s) 140
BstV1I GCAGC 6 cut(s) 203, 446, 625, 762, 832, 860
BstV2I GAAGAC 1 cut(s) 494
BstXI CCANNNNNNTGG 1 cut(s) 788
BstZI CGGCCG 1 cut(s) 924
Bsu15I ATCGAT 1 cut(s) 87
BsuRI GGCC 7 cut(s) 138, 156, 412, 731, 926, 1029, 1292
BsuTUI ATCGAT 1 cut(s) 87
BtgI CCRYGG 1 cut(s) 1336
BtsCI GGATG 3 cut(s) 376, 541, 745
BtsI GCAGTG 3 cut(s) 984, 1188, 1414
BtsIMutI CAGTG 6 cut(s) 65, 486, 984, 1188, 1271, 1414
BveI ACCTGC 1 cut(s) 818
Cac8I GCNNGC 3 cut(s) 748, 843, 1186
CaiI CAGNNNCTG 1 cut(s) 983
CciI TCATGA 1 cut(s) 955
CfoI GCGC 2 cut(s) 518, 1407
Cfr13I GGNCC 6 cut(s) 136, 137, 410, 484, 729, 1028
ClaI ATCGAT 1 cut(s) 87
CseI GACGC 1 cut(s) 1000
Csp6I GTAC 3 cut(s) 161, 665, 831
CviAII CATG 7 cut(s) 158, 642, 864, 956, 1086, 1132, 1139
CviQI GTAC 3 cut(s) 161, 665, 831
DdeI CTNAG 4 cut(s) 184, 220, 352, 1007
DpnI GATC 3 cut(s) 702, 816, 1313
DpnII GATC 3 cut(s) 700, 814, 1311
EaeI YGGCCR 3 cut(s) 154, 924, 1290
EagI CGGCCG 1 cut(s) 924
Eam1104I CTCTTC 4 cut(s) 60, 562, 1386, 1431
EarI CTCTTC 4 cut(s) 60, 562, 1386, 1431
EclXI CGGCCG 1 cut(s) 924
Eco130I CCWWGG 1 cut(s) 963
Eco24I GRGCYC 2 cut(s) 140, 475
Eco47I GGWCC 1 cut(s) 484
Eco52I CGGCCG 1 cut(s) 924
Eco88I CYCGRG 1 cut(s) 604
EcoO109I RGGNCCY 2 cut(s) 137, 729
EcoRII CCWGG 1 cut(s) 1036
EcoT14I CCWWGG 1 cut(s) 963
EcoT22I ATGCAT 1 cut(s) 869
EcoT38I GRGCYC 2 cut(s) 140, 475
ErhI CCWWGG 1 cut(s) 963
FaeI CATG 7 cut(s) 161, 645, 867, 959, 1089, 1135, 1142
FatI CATG 7 cut(s) 157, 641, 863, 955, 1085, 1131, 1138
FauNDI CATATG 1 cut(s) 553
FokI GGATG 3 cut(s) 383, 548, 752
FriOI GRGCYC 2 cut(s) 140, 475
FspBI CTAG 1 cut(s) 708
GlaI GCGC 2 cut(s) 517, 1406
GsaI CCCAGC 2 cut(s) 148, 1287
HaeII RGCGCY 1 cut(s) 519
HaeIII GGCC 7 cut(s) 138, 156, 412, 731, 926, 1029, 1292
HapII CCGG 4 cut(s) 74, 153, 248, 453
HgaI GACGC 1 cut(s) 1000
HhaI GCGC 2 cut(s) 518, 1407
Hin1II CATG 7 cut(s) 161, 645, 867, 959, 1089, 1135, 1142
Hin6I GCGC 2 cut(s) 516, 1405
HinP1I GCGC 2 cut(s) 516, 1405
HincII GTYRAC 1 cut(s) 334
HindII GTYRAC 1 cut(s) 334
HindIII AAGCTT 2 cut(s) 758, 1157
HinfI GANTC 7 cut(s) 70, 196, 235, 426, 1112, 1135, 1171
HpaI GTTAAC 1 cut(s) 334
HpaII CCGG 4 cut(s) 74, 153, 248, 453
HphI GGTGA 3 cut(s) 21, 870, 1007
Hpy166II GTNNAC 2 cut(s) 334, 484
Hpy188III TCNNGA 3 cut(s) 943, 956, 1225
Hpy8I GTNNAC 2 cut(s) 334, 484
Hpy99I CGWCG 1 cut(s) 1016
HpyAV CCTTC 3 cut(s) 19, 150, 1292
HpyCH4III ACNGT 4 cut(s) 664, 669, 725, 1337
HpyCH4IV ACGT 1 cut(s) 243
HpyCH4V TGCA 9 cut(s) 446, 462, 641, 809, 848, 867, 936, 1089, 1247
HpyF10VI GCNNNNNNNGC 7 cut(s) 222, 443, 557, 647, 1190, 1289, 1413
HpyF3I CTNAG 4 cut(s) 184, 220, 352, 1007
HpySE526I ACGT 1 cut(s) 243
Hsp92II CATG 7 cut(s) 161, 645, 867, 959, 1089, 1135, 1142
HspAI GCGC 2 cut(s) 516, 1405
KspAI GTTAAC 1 cut(s) 334
Kzo9I GATC 3 cut(s) 700, 814, 1311
LguI GCTCTTC 1 cut(s) 1431
LmnI GCTCC 6 cut(s) 179, 296, 743, 1163, 1376, 1402
Lsp1109I GCAGC 6 cut(s) 203, 446, 625, 762, 832, 860
LweI GCATC 1 cut(s) 876
MaeI CTAG 1 cut(s) 708
MaeII ACGT 1 cut(s) 243
MaeIII GTNAC 1 cut(s) 1269
MalI GATC 3 cut(s) 702, 816, 1313
MboI GATC 3 cut(s) 700, 814, 1311
MboII GAAGA 5 cut(s) 47, 494, 579, 1403, 1418
MhlI GDGCHC 3 cut(s) 140, 475, 1190
MluCI AATT 5 cut(s) 375, 527, 852, 1000, 1348
MlyI GAGTC 1 cut(s) 1144
MmeI TCCRAC 3 cut(s) 559, 616, 1081
Mph1103I ATGCAT 1 cut(s) 869
MseI TTAA 3 cut(s) 333, 1020, 1097
MspA1I CMGCKG 3 cut(s) 171, 929, 1317
MspI CCGG 4 cut(s) 74, 153, 248, 453
MspR9I CCNGG 2 cut(s) 453, 1038
Mva1269I GAATGC 2 cut(s) 803, 1416
MvaI CCWGG 1 cut(s) 1038
MwoI GCNNNNNNNGC 7 cut(s) 222, 443, 557, 647, 1190, 1289, 1413
NciI CCSGG 1 cut(s) 453
NdeI CATATG 1 cut(s) 553
NdeII GATC 3 cut(s) 700, 814, 1311
NlaIII CATG 7 cut(s) 161, 645, 867, 959, 1089, 1135, 1142
NlaIV GGNNCC 5 cut(s) 78, 138, 175, 730, 1234
NmuCI GTSAC 1 cut(s) 1269
NsiI ATGCAT 1 cut(s) 869
NspI RCATGY 2 cut(s) 645, 1089
PagI TCATGA 1 cut(s) 955
PciSI GCTCTTC 1 cut(s) 1431
PctI GAATGC 2 cut(s) 803, 1416
PfeI GAWTC 6 cut(s) 70, 196, 235, 426, 1112, 1171
PflMI CCANNNNNTGG 1 cut(s) 1037
PleI GAGTC 1 cut(s) 1143
PpsI GAGTC 1 cut(s) 1143
Psp6I CCWGG 1 cut(s) 1036
PspFI CCCAGC 2 cut(s) 144, 1283
PspGI CCWGG 1 cut(s) 1036
PspN4I GGNNCC 5 cut(s) 78, 138, 175, 730, 1234
PspOMI GGGCCC 1 cut(s) 136
PspPI GGNCC 6 cut(s) 136, 137, 410, 484, 729, 1028
PstI CTGCAG 1 cut(s) 850
PstNI CAGNNNCTG 1 cut(s) 983
RsaI GTAC 3 cut(s) 162, 666, 832
RsaNI GTAC 3 cut(s) 161, 665, 831
SapI GCTCTTC 1 cut(s) 1431
SaqAI TTAA 3 cut(s) 333, 1020, 1097
Sau3AI GATC 3 cut(s) 700, 814, 1311
Sau96I GGNCC 6 cut(s) 136, 137, 410, 484, 729, 1028
SchI GAGTC 1 cut(s) 1144
ScrFI CCNGG 2 cut(s) 453, 1038
SduI GDGCHC 3 cut(s) 140, 475, 1190
SfaNI GCATC 1 cut(s) 876
SfcI CTRYAG 3 cut(s) 846, 1104, 1305
SinI GGWCC 1 cut(s) 484
Sse9I AATT 5 cut(s) 375, 527, 852, 1000, 1348
SsiI CCGC 8 cut(s) 171, 203, 368, 437, 558, 927, 1293, 1317
SspI AATATT 1 cut(s) 466
SspMI CTAG 1 cut(s) 708
StyD4I CCNGG 2 cut(s) 451, 1036
StyI CCWWGG 1 cut(s) 963
TaaI ACNGT 4 cut(s) 664, 669, 725, 1337
TaiI ACGT 1 cut(s) 246
TaqI TCGA 5 cut(s) 87, 258, 306, 475, 1389
TasI AATT 5 cut(s) 375, 527, 852, 1000, 1348
TatI WGTACW 1 cut(s) 664
TauI GCSGC 5 cut(s) 174, 206, 560, 929, 1295
TfiI GAWTC 6 cut(s) 70, 196, 235, 426, 1112, 1171
Tru1I TTAA 3 cut(s) 333, 1020, 1097
Tru9I TTAA 3 cut(s) 333, 1020, 1097
TscAI CASTG 6 cut(s) 72, 486, 984, 1195, 1278, 1414
TseFI GTSAC 1 cut(s) 1269
TseI GCWGC 6 cut(s) 216, 459, 638, 750, 845, 848
Tsp45I GTSAC 1 cut(s) 1269
TspDTI ATGAA 4 cut(s) 387, 1373, 1398, 1418
TspGWI ACGGA 1 cut(s) 635
TspRI CASTG 6 cut(s) 72, 486, 984, 1195, 1278, 1414
Van91I CCANNNNNTGG 1 cut(s) 1037
VpaK11BI GGWCC 1 cut(s) 484
XapI RAATTY 3 cut(s) 375, 1000, 1348
XceI RCATGY 2 cut(s) 645, 1089
XspI CTAG 1 cut(s) 708
Zsp2I ATGCAT 1 cut(s) 869
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.