Rroxscaffold_4G00315890

EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001544
Physical Location & Seq
Reverse (-)
40448709 .. 40450636
1928 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00315890.1

Sequence Viewer

Length: 579 bp
ATGTCTGGTGAAAACAAGGCAGGGGAATGGTCTGGGGCCTGTGTGGAAGGAGCAGGAAGCAACAAGCTTGTCATCAAGGGAAAATCTCATTTGCATGGTTGTAAATGTACTATTGCACCTGATTGTATTGAAGCAGGCACATTTATGCTTGCTGCAGCTATTACTCGCTCATTCATTTCAATTTCACCTGTCATTCCTTACCAAGTTTCCTGTCTGATGCAAAAACTCTTGGCCGCTGGTTGCAAAATAAGGCAATGCTCTCATGATACCTTGGAAGTTTCAGCAGTGTCTCGATGTGGTGAGAATTTGCGAGGTTTTGAGGTTAAGACAGGGCCATTTCCCGGGTTTCCTACAGATCTCCAACCGCAAACAATGGCACTGCTAGCCACTTGTGGTTTGTCTGGTTCCTGCCTTGCTGCAACTGACCTCAGAGGTGGGATATCATTGGCATTGGCTGCAGAAGGTGCTACTGAGATCAGCGGTGTTGCTCATATTGACCGTGGTTATGAGAATGTAGATACAAAAGTTCATATGCTGGGAGCCGATATCAAAAGATTAACGGCCCCTGCTTCTACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPSP_synthase

Protein Analysis

192

Amino Acids

19.91

Weight (kDa)

6.93

Isoelectric Point (pI)

36.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPSP_synthase PF00275 12 - 128 2.5e-16 EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 234, 365, 480
AcoI YGGCCR 1 cut(s) 231
AcsI RAATTY 1 cut(s) 304
AfaI GTAC 1 cut(s) 109
AfiI CCNNNNNNNGG 1 cut(s) 341
AgsI TTSAA 2 cut(s) 131, 180
AluBI AGCT 2 cut(s) 67, 158
AluI AGCT 2 cut(s) 67, 158
Alw26I GTCTC 1 cut(s) 294
Ama87I CYCGRG 1 cut(s) 341
AoxI GGCC 4 cut(s) 36, 231, 332, 561
ApeKI GCWGC 4 cut(s) 152, 155, 416, 455
ApoI RAATTY 1 cut(s) 304
AspS9I GGNCC 3 cut(s) 36, 332, 562
AsuC2I CCSGG 2 cut(s) 342, 343
AsuHPI GGTGA 3 cut(s) 20, 177, 311
AsuNHI GCTAGC 1 cut(s) 382
AvaI CYCGRG 1 cut(s) 341
BaeI ACNNNNGTAYC 2 cut(s) 510, 543
BbvI GCAGC 4 cut(s) 139, 167, 403, 442
BceAI ACGGC 1 cut(s) 576
BcnI CCSGG 2 cut(s) 342, 343
BcoDI GTCTC 1 cut(s) 294
BfaI CTAG 2 cut(s) 383, 577
BfmI CTRYAG 3 cut(s) 153, 351, 456
BglII AGATCT 1 cut(s) 355
BisI GCNGC 5 cut(s) 153, 156, 234, 417, 456
BlsI GCNGC 5 cut(s) 154, 157, 235, 418, 457
Bme1390I CCNGG 2 cut(s) 342, 343
BmeT110I CYCGRG 1 cut(s) 341
BmgT120I GGNCC 3 cut(s) 36, 332, 562
BmiI GGNNCC 4 cut(s) 37, 406, 541, 564
BmrFI CCNGG 2 cut(s) 342, 343
BmsI GCATC 1 cut(s) 207
BmtI GCTAGC 1 cut(s) 386
BpuMI CCSGG 2 cut(s) 342, 343
BsaJI CCNNGG 3 cut(s) 270, 341, 499
Bsc4I CCNNNNNNNGG 1 cut(s) 341
Bse3DI GCAATG 1 cut(s) 260
BseDI CCNNGG 3 cut(s) 270, 341, 499
BseLI CCNNNNNNNGG 1 cut(s) 341
BseMI GCAATG 1 cut(s) 260
BseMII CTCAG 2 cut(s) 442, 462
BseXI GCAGC 4 cut(s) 139, 167, 403, 442
BseYI CCCAGC 1 cut(s) 535
BshFI GGCC 4 cut(s) 38, 233, 334, 563
BsiHKCI CYCGRG 1 cut(s) 341
BsiSI CCGG 1 cut(s) 342
BslI CCNNNNNNNGG 1 cut(s) 341
BsmAI GTCTC 1 cut(s) 294
BsnI GGCC 4 cut(s) 38, 233, 334, 563
BsoBI CYCGRG 1 cut(s) 341
Bsp143I GATC 2 cut(s) 355, 474
BspACI CCGC 3 cut(s) 234, 365, 480
BspANI GGCC 4 cut(s) 38, 233, 334, 563
BspCNI CTCAG 2 cut(s) 441, 463
BspHI TCATGA 1 cut(s) 262
BspLI GGNNCC 4 cut(s) 37, 406, 541, 564
BspMAI CTGCAG 2 cut(s) 157, 460
BspOI GCTAGC 1 cut(s) 386
BsrDI GCAATG 1 cut(s) 260
BssECI CCNNGG 3 cut(s) 270, 341, 499
BssMI GATC 2 cut(s) 355, 474
BssT1I CCWWGG 1 cut(s) 270
Bst4CI ACNGT 1 cut(s) 500
BstAPI GCANNNNNTGC 2 cut(s) 455, 464
BstC8I GCNNGC 3 cut(s) 136, 150, 384
BstDEI CTNAG 2 cut(s) 428, 471
BstDSI CCRYGG 1 cut(s) 499
BstKTI GATC 2 cut(s) 358, 477
BstMAI GTCTC 1 cut(s) 294
BstMBI GATC 2 cut(s) 355, 474
BstMWI GCNNNNNNNGC 3 cut(s) 383, 455, 464
BstSCI CCNGG 2 cut(s) 340, 341
BstSFI CTRYAG 3 cut(s) 153, 351, 456
BstV1I GCAGC 4 cut(s) 139, 167, 403, 442
BstX2I RGATCY 1 cut(s) 355
BstYI RGATCY 1 cut(s) 355
BsuRI GGCC 4 cut(s) 38, 233, 334, 563
BtgI CCRYGG 1 cut(s) 499
BtsI GCAGTG 2 cut(s) 291, 377
BtsIMutI CAGTG 2 cut(s) 291, 377
Cac8I GCNNGC 3 cut(s) 136, 150, 384
CciI TCATGA 1 cut(s) 262
Cfr13I GGNCC 3 cut(s) 36, 332, 562
Cfr9I CCCGGG 1 cut(s) 341
Csp6I GTAC 1 cut(s) 108
CviAII CATG 2 cut(s) 95, 263
CviJI RGCY 9 cut(s) 38, 67, 158, 233, 334, 386, 455, 542, 563
CviKI_1 RGCY 9 cut(s) 38, 67, 158, 233, 334, 386, 455, 542, 563
CviQI GTAC 1 cut(s) 108
DdeI CTNAG 2 cut(s) 428, 471
DpnI GATC 2 cut(s) 357, 476
DpnII GATC 2 cut(s) 355, 474
EaeI YGGCCR 1 cut(s) 231
Eco130I CCWWGG 1 cut(s) 270
Eco32I GATATC 2 cut(s) 441, 547
Eco88I CYCGRG 1 cut(s) 341
EcoO109I RGGNCCY 1 cut(s) 36
EcoRV GATATC 2 cut(s) 441, 547
EcoT14I CCWWGG 1 cut(s) 270
ErhI CCWWGG 1 cut(s) 270
FaeI CATG 2 cut(s) 98, 266
FaiI YATR 7 cut(s) 96, 146, 264, 492, 507, 531, 533
FatI CATG 2 cut(s) 94, 262
FauNDI CATATG 1 cut(s) 531
Fnu4HI GCNGC 5 cut(s) 153, 156, 234, 417, 456
Fsp4HI GCNGC 5 cut(s) 153, 156, 234, 417, 456
FspBI CTAG 2 cut(s) 383, 577
GluI GCNGC 5 cut(s) 153, 156, 234, 417, 456
GsaI CCCAGC 1 cut(s) 539
HaeIII GGCC 4 cut(s) 38, 233, 334, 563
HapII CCGG 1 cut(s) 342
Hin1II CATG 2 cut(s) 98, 266
HindIII AAGCTT 1 cut(s) 65
HpaII CCGG 1 cut(s) 342
HphI GGTGA 3 cut(s) 20, 177, 311
Hpy188I TCNGA 2 cut(s) 216, 431
Hpy188III TCNNGA 2 cut(s) 263, 291
HpyAV CCTTC 2 cut(s) 41, 455
HpyCH4III ACNGT 1 cut(s) 500
HpyCH4V TGCA 7 cut(s) 94, 116, 155, 220, 243, 419, 458
HpyF10VI GCNNNNNNNGC 3 cut(s) 383, 455, 464
HpyF3I CTNAG 2 cut(s) 428, 471
Hsp92II CATG 2 cut(s) 98, 266
Kzo9I GATC 2 cut(s) 355, 474
LmnI GCTCC 2 cut(s) 50, 539
Lsp1109I GCAGC 4 cut(s) 139, 167, 403, 442
LweI GCATC 1 cut(s) 207
MaeI CTAG 2 cut(s) 383, 577
MalI GATC 2 cut(s) 357, 476
MboI GATC 2 cut(s) 355, 474
MflI RGATCY 1 cut(s) 355
MluCI AATT 2 cut(s) 180, 304
MmeI TCCRAC 1 cut(s) 385
MnlI CCTC 4 cut(s) 305, 313, 425, 437
MseI TTAA 2 cut(s) 324, 557
MslI CAYNNNNRTG 2 cut(s) 93, 143
MspA1I CMGCKG 2 cut(s) 236, 480
MspI CCGG 1 cut(s) 342
MspR9I CCNGG 2 cut(s) 342, 343
MwoI GCNNNNNNNGC 3 cut(s) 383, 455, 464
NciI CCSGG 2 cut(s) 342, 343
NdeI CATATG 1 cut(s) 531
NdeII GATC 2 cut(s) 355, 474
NheI GCTAGC 1 cut(s) 382
NlaIII CATG 2 cut(s) 98, 266
NlaIV GGNNCC 4 cut(s) 37, 406, 541, 564
PagI TCATGA 1 cut(s) 262
PkrI GCNGC 5 cut(s) 154, 157, 235, 418, 457
PspFI CCCAGC 1 cut(s) 535
PspN4I GGNNCC 4 cut(s) 37, 406, 541, 564
PspPI GGNCC 3 cut(s) 36, 332, 562
PstI CTGCAG 2 cut(s) 157, 460
PsuI RGATCY 1 cut(s) 355
RsaI GTAC 1 cut(s) 109
RsaNI GTAC 1 cut(s) 108
RseI CAYNNNNRTG 2 cut(s) 93, 143
SaqAI TTAA 2 cut(s) 324, 557
SatI GCNGC 5 cut(s) 153, 156, 234, 417, 456
Sau3AI GATC 2 cut(s) 355, 474
Sau96I GGNCC 3 cut(s) 36, 332, 562
ScrFI CCNGG 2 cut(s) 342, 343
SfaNI GCATC 1 cut(s) 207
SfcI CTRYAG 3 cut(s) 153, 351, 456
SmaI CCCGGG 1 cut(s) 343
SmiMI CAYNNNNRTG 2 cut(s) 93, 143
Sse9I AATT 2 cut(s) 180, 304
SsiI CCGC 3 cut(s) 234, 365, 480
SspMI CTAG 2 cut(s) 383, 577
StyD4I CCNGG 2 cut(s) 340, 341
StyI CCWWGG 1 cut(s) 270
TaaI ACNGT 1 cut(s) 500
TaqI TCGA 1 cut(s) 292
TasI AATT 2 cut(s) 180, 304
TatI WGTACW 1 cut(s) 107
TauI GCSGC 1 cut(s) 236
Tru1I TTAA 2 cut(s) 324, 557
Tru9I TTAA 2 cut(s) 324, 557
TscAI CASTG 2 cut(s) 291, 384
TseI GCWGC 4 cut(s) 152, 155, 416, 455
TspDTI ATGAA 2 cut(s) 163, 518
TspMI CCCGGG 1 cut(s) 341
TspRI CASTG 2 cut(s) 291, 384
XapI RAATTY 1 cut(s) 304
XmaI CCCGGG 1 cut(s) 341
XspI CTAG 2 cut(s) 383, 577
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.