RLG00000010063

EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0001544
Physical Location & Seq
Forward (+)
62582377 .. 62582950
574 bp
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UTR
Exon/CDS
Intron
RLM00000010063

Sequence Viewer

Length: 465 bp
ATGTTCAAGTGTTGTGGTCTGCTGTCTAATGGATTTTGTAAAGTGACCTGTTTTCGCCAACATTTTCCCACAGGAACCGAGGTGGTTGATCTTGCTCGATTTCTGACTAAAAGTGGGGCCTTTGTGGAAGGAGCAGGAAGCAACAAGCTTGTCATCAAGGGAAAATCTCATTTGCATGGTTGTAAATGTACTATTGCACCTGATCGTATTGAAGCAGGCACATTTATGCTTGCTGCAGCTATTACTCGCTCATTCATTTCAATTTCACCTGTCATTCCTTCCCAAGTTTCCTGTCTGATGCAAAAACTCTTAGCCGCGGGTTGCAAAATAAGGCAATGCTGTCATGATACCGTGGAAGTGTCAGCAGTGTCTCGATGTGTGAGAATTTGCGAGGTTTTGAGGTTAAGACAGGGCCATTTCCCGGGTTTCCTACAGATCTTCAACCGCAAACAATGGCACTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPSP_synthase

Protein Analysis

155

Amino Acids

16.96

Weight (kDa)

9.37

Isoelectric Point (pI)

33.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPSP_synthase PF00275 20 - 122 9.9e-17 EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 317
AciI CCGC 3 cut(s) 315, 317, 445
AcsI RAATTY 1 cut(s) 384
AfaI GTAC 1 cut(s) 190
AfiI CCNNNNNNNGG 1 cut(s) 421
AgsI TTSAA 4 cut(s) 7, 212, 261, 442
AluBI AGCT 2 cut(s) 148, 239
AluI AGCT 2 cut(s) 148, 239
Alw26I GTCTC 1 cut(s) 375
Ama87I CYCGRG 1 cut(s) 421
AoxI GGCC 2 cut(s) 117, 412
ApeKI GCWGC 2 cut(s) 233, 236
ApoI RAATTY 1 cut(s) 384
AspS9I GGNCC 2 cut(s) 117, 412
AsuC2I CCSGG 2 cut(s) 422, 423
AsuHPI GGTGA 1 cut(s) 258
AvaI CYCGRG 1 cut(s) 421
BbvI GCAGC 2 cut(s) 220, 248
BcnI CCSGG 2 cut(s) 422, 423
BcoDI GTCTC 1 cut(s) 375
BfmI CTRYAG 2 cut(s) 234, 431
BglII AGATCT 1 cut(s) 435
BisI GCNGC 3 cut(s) 234, 237, 315
BlsI GCNGC 3 cut(s) 235, 238, 316
Bme1390I CCNGG 2 cut(s) 422, 423
BmeT110I CYCGRG 1 cut(s) 421
BmgT120I GGNCC 2 cut(s) 117, 412
BmiI GGNNCC 2 cut(s) 76, 118
BmrFI CCNGG 2 cut(s) 422, 423
BmsI GCATC 1 cut(s) 288
BpuMI CCSGG 2 cut(s) 422, 423
BsaJI CCNNGG 4 cut(s) 78, 315, 351, 421
Bsc4I CCNNNNNNNGG 1 cut(s) 421
Bse3DI GCAATG 1 cut(s) 341
BseDI CCNNGG 4 cut(s) 78, 315, 351, 421
BseLI CCNNNNNNNGG 1 cut(s) 421
BseMI GCAATG 1 cut(s) 341
BseXI GCAGC 2 cut(s) 220, 248
Bsh1236I CGCG 1 cut(s) 317
BshFI GGCC 2 cut(s) 119, 414
BsiHKCI CYCGRG 1 cut(s) 421
BsiSI CCGG 1 cut(s) 422
BslI CCNNNNNNNGG 1 cut(s) 421
BsmAI GTCTC 1 cut(s) 375
BsnI GGCC 2 cut(s) 119, 414
BsoBI CYCGRG 1 cut(s) 421
Bsp143I GATC 3 cut(s) 88, 202, 435
BspACI CCGC 3 cut(s) 315, 317, 445
BspANI GGCC 2 cut(s) 119, 414
BspFNI CGCG 1 cut(s) 317
BspHI TCATGA 1 cut(s) 343
BspLI GGNNCC 2 cut(s) 76, 118
BspMAI CTGCAG 1 cut(s) 238
BsrDI GCAATG 1 cut(s) 341
BssECI CCNNGG 4 cut(s) 78, 315, 351, 421
BssMI GATC 3 cut(s) 88, 202, 435
Bst4CI ACNGT 1 cut(s) 352
BstC8I GCNNGC 2 cut(s) 217, 231
BstDEI CTNAG 1 cut(s) 310
BstDSI CCRYGG 2 cut(s) 315, 351
BstFNI CGCG 1 cut(s) 317
BstKTI GATC 3 cut(s) 91, 205, 438
BstMAI GTCTC 1 cut(s) 375
BstMBI GATC 3 cut(s) 88, 202, 435
BstSCI CCNGG 2 cut(s) 420, 421
BstSFI CTRYAG 2 cut(s) 234, 431
BstUI CGCG 1 cut(s) 317
BstV1I GCAGC 2 cut(s) 220, 248
BstX2I RGATCY 1 cut(s) 435
BstYI RGATCY 1 cut(s) 435
BsuRI GGCC 2 cut(s) 119, 414
BtgI CCRYGG 2 cut(s) 315, 351
BtsI GCAGTG 2 cut(s) 372, 457
BtsIMutI CAGTG 2 cut(s) 372, 457
Cac8I GCNNGC 2 cut(s) 217, 231
CciI TCATGA 1 cut(s) 343
Cfr13I GGNCC 2 cut(s) 117, 412
Cfr42I CCGCGG 1 cut(s) 318
Cfr9I CCCGGG 1 cut(s) 421
Csp6I GTAC 1 cut(s) 189
CspCI CAANNNNNGTGG 1 cut(s) 30
CviAII CATG 2 cut(s) 176, 344
CviJI RGCY 5 cut(s) 119, 148, 239, 314, 414
CviKI_1 RGCY 5 cut(s) 119, 148, 239, 314, 414
CviQI GTAC 1 cut(s) 189
DdeI CTNAG 1 cut(s) 310
DpnI GATC 3 cut(s) 90, 204, 437
DpnII GATC 3 cut(s) 88, 202, 435
Eco88I CYCGRG 1 cut(s) 421
EcoO109I RGGNCCY 1 cut(s) 117
FaeI CATG 2 cut(s) 179, 347
FaiI YATR 3 cut(s) 177, 227, 345
FatI CATG 2 cut(s) 175, 343
FauI CCCGC 1 cut(s) 310
Fnu4HI GCNGC 3 cut(s) 234, 237, 315
Fsp4HI GCNGC 3 cut(s) 234, 237, 315
GluI GCNGC 3 cut(s) 234, 237, 315
HaeIII GGCC 2 cut(s) 119, 414
HapII CCGG 1 cut(s) 422
Hin1II CATG 2 cut(s) 179, 347
HindIII AAGCTT 1 cut(s) 146
HpaII CCGG 1 cut(s) 422
HphI GGTGA 1 cut(s) 258
Hpy188I TCNGA 2 cut(s) 105, 297
Hpy188III TCNNGA 2 cut(s) 344, 372
HpyAV CCTTC 2 cut(s) 122, 288
HpyCH4III ACNGT 1 cut(s) 352
HpyCH4V TGCA 5 cut(s) 175, 197, 236, 301, 324
HpyF3I CTNAG 1 cut(s) 310
Hsp92II CATG 2 cut(s) 179, 347
KspI CCGCGG 1 cut(s) 318
Kzo9I GATC 3 cut(s) 88, 202, 435
LmnI GCTCC 1 cut(s) 131
LpnPI CCDG 9 cut(s) 57, 61, 120, 201, 213, 282, 304, 395, 435
Lsp1109I GCAGC 2 cut(s) 220, 248
LweI GCATC 1 cut(s) 288
MaeIII GTNAC 1 cut(s) 43
MalI GATC 3 cut(s) 90, 204, 437
MboI GATC 3 cut(s) 88, 202, 435
MboII GAAGA 1 cut(s) 430
MflI RGATCY 1 cut(s) 435
MluCI AATT 2 cut(s) 261, 384
MnlI CCTC 3 cut(s) 73, 385, 393
MseI TTAA 1 cut(s) 404
MslI CAYNNNNRTG 2 cut(s) 174, 224
MspA1I CMGCKG 1 cut(s) 317
MspI CCGG 1 cut(s) 422
MspR9I CCNGG 2 cut(s) 422, 423
MvnI CGCG 1 cut(s) 317
NciI CCSGG 2 cut(s) 422, 423
NdeII GATC 3 cut(s) 88, 202, 435
NlaIII CATG 2 cut(s) 179, 347
NlaIV GGNNCC 2 cut(s) 76, 118
NmuCI GTSAC 1 cut(s) 43
PagI TCATGA 1 cut(s) 343
PkrI GCNGC 3 cut(s) 235, 238, 316
PspN4I GGNNCC 2 cut(s) 76, 118
PspPI GGNCC 2 cut(s) 117, 412
PstI CTGCAG 1 cut(s) 238
PsuI RGATCY 1 cut(s) 435
RsaI GTAC 1 cut(s) 190
RsaNI GTAC 1 cut(s) 189
RseI CAYNNNNRTG 2 cut(s) 174, 224
SacII CCGCGG 1 cut(s) 318
SaqAI TTAA 1 cut(s) 404
SatI GCNGC 3 cut(s) 234, 237, 315
Sau3AI GATC 3 cut(s) 88, 202, 435
Sau96I GGNCC 2 cut(s) 117, 412
ScrFI CCNGG 2 cut(s) 422, 423
SetI ASST 8 cut(s) 50, 84, 150, 202, 241, 271, 396, 404
SfaNI GCATC 1 cut(s) 288
SfcI CTRYAG 2 cut(s) 234, 431
Sfr303I CCGCGG 1 cut(s) 318
SgrBI CCGCGG 1 cut(s) 318
SmaI CCCGGG 1 cut(s) 423
SmiMI CAYNNNNRTG 2 cut(s) 174, 224
Sse9I AATT 2 cut(s) 261, 384
SsiI CCGC 3 cut(s) 315, 317, 445
StyD4I CCNGG 2 cut(s) 420, 421
TaaI ACNGT 1 cut(s) 352
TaqI TCGA 2 cut(s) 97, 373
TasI AATT 2 cut(s) 261, 384
TatI WGTACW 1 cut(s) 188
TauI GCSGC 1 cut(s) 317
Tru1I TTAA 1 cut(s) 404
Tru9I TTAA 1 cut(s) 404
TscAI CASTG 2 cut(s) 372, 464
TseFI GTSAC 1 cut(s) 43
TseI GCWGC 2 cut(s) 233, 236
Tsp45I GTSAC 1 cut(s) 43
TspDTI ATGAA 1 cut(s) 244
TspMI CCCGGG 1 cut(s) 421
TspRI CASTG 2 cut(s) 372, 464
XapI RAATTY 1 cut(s) 384
XmaI CCCGGG 1 cut(s) 421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.