Rh5CG241300

EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0001544
Physical Location & Seq
Reverse (-)
25071185 .. 25075178
3994 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG241300.1

Sequence Viewer

Length: 732 bp
ATGAAGGATGGAAAAGTTCAGGCATATGCATTAAATGGAAGAGGATTGGTTGGGGGAAGTTTCCAACTTAATTACCCTAGCGTTGGGGCAACAGAAACCCTTCTGATGGCTGCATGTATGGCTGATGGAACAACTATACTGTCCAGTGTTGCCAGACAGGAAGCAACAAAGCTTGTCATAAAGGGAAAATCTCATTTTCATGGTTGTAAATGTACTATTGCACCTGATTGTATTGAAGCAGGCACGTTTTTGCTTGCTGCAGCTATTGCTTGCTCATTCATTTCAATTTCACCTGTCATTCCTTCCCAAGTTTCCTGTCTGATGCAAAAACTCTTGGCCACTTGTTGCAAAATAAGGCAATGCTCTCATGATACCTTGGAAGTATTTTCAGAAGTGTCTCAATGTGGTGAGAATTTGCGAGGTTTTGAGGTTAAGACAGGGCCATTTCCCGGGTTTCCTACAAATCTCCAACCTCGAACAATGGCCCTGCTAACCACATGCAATGGTTTAAGTCCGGTAGAAGAGTCTGTATTTGAAAAGCGCATGGCAAATGTGGTTTGTCTGGTTCCTGCCTTGCTGCAACTGACCTCATTGGTATTAGCTGCATTGGCTGCAGAAGGTACTACTGAGATCAGCAGTGTTGCTCATATTGACCGTGGTTATGAGAATGTAGATACACTTCATATGCTGGGAGCCGATATCAAAAGATTAATGGCCCCTGCTTCTTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPSP_synthase

Protein Analysis

243

Amino Acids

25.72

Weight (kDa)

6.51

Isoelectric Point (pI)

44.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPSP_synthase PF00275 53 - 185 7.6e-14 EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 336
AcsI RAATTY 1 cut(s) 412
AfaI GTAC 2 cut(s) 214, 622
AfiI CCNNNNNNNGG 3 cut(s) 83, 106, 449
AgsI TTSAA 3 cut(s) 236, 285, 536
AluBI AGCT 3 cut(s) 172, 263, 602
AluI AGCT 3 cut(s) 172, 263, 602
Alw26I GTCTC 1 cut(s) 402
Ama87I CYCGRG 1 cut(s) 449
AoxI GGCC 4 cut(s) 336, 440, 483, 714
ApeKI GCWGC 6 cut(s) 110, 257, 260, 577, 602, 611
ApoI RAATTY 1 cut(s) 412
AseI ATTAAT 1 cut(s) 710
Asp700I GAANNNNTTC 1 cut(s) 99
AspLEI GCGC 1 cut(s) 543
AspS9I GGNCC 3 cut(s) 440, 484, 715
AsuC2I CCSGG 2 cut(s) 450, 451
AsuHPI GGTGA 2 cut(s) 282, 419
AvaI CYCGRG 1 cut(s) 449
BalI TGGCCA 1 cut(s) 338
BarI GAAGNNNNNNTAC 2 cut(s) 663, 695
BbvI GCAGC 6 cut(s) 97, 244, 272, 564, 589, 598
BccI CCATC 3 cut(s) 2, 100, 119
BcnI CCSGG 2 cut(s) 450, 451
BcoDI GTCTC 1 cut(s) 402
BfaI CTAG 2 cut(s) 78, 730
BfmI CTRYAG 2 cut(s) 258, 612
BisI GCNGC 6 cut(s) 111, 258, 261, 578, 603, 612
BlsI GCNGC 6 cut(s) 112, 259, 262, 579, 604, 613
Bme1390I CCNGG 2 cut(s) 450, 451
BmeT110I CYCGRG 1 cut(s) 449
BmgT120I GGNCC 3 cut(s) 440, 484, 715
BmiI GGNNCC 3 cut(s) 567, 694, 717
BmrFI CCNGG 2 cut(s) 450, 451
BmsI GCATC 1 cut(s) 312
BpuMI CCSGG 2 cut(s) 450, 451
BsaJI CCNNGG 3 cut(s) 375, 449, 655
BsaWI WCCGGW 1 cut(s) 514
Bsc4I CCNNNNNNNGG 3 cut(s) 83, 106, 449
Bse1I ACTGG 1 cut(s) 144
Bse3DI GCAATG 2 cut(s) 365, 508
BseDI CCNNGG 3 cut(s) 375, 449, 655
BseGI GGATG 1 cut(s) 13
BseLI CCNNNNNNNGG 3 cut(s) 83, 106, 449
BseMI GCAATG 2 cut(s) 365, 508
BseMII CTCAG 1 cut(s) 618
BseNI ACTGG 1 cut(s) 144
BseXI GCAGC 6 cut(s) 97, 244, 272, 564, 589, 598
BseYI CCCAGC 1 cut(s) 688
BshFI GGCC 4 cut(s) 338, 442, 485, 716
BsiHKCI CYCGRG 1 cut(s) 449
BsiSI CCGG 2 cut(s) 450, 515
BslI CCNNNNNNNGG 3 cut(s) 83, 106, 449
BsmAI GTCTC 1 cut(s) 402
BsnI GGCC 4 cut(s) 338, 442, 485, 716
BsoBI CYCGRG 1 cut(s) 449
Bsp143I GATC 1 cut(s) 630
BspANI GGCC 4 cut(s) 338, 442, 485, 716
BspCNI CTCAG 1 cut(s) 619
BspHI TCATGA 1 cut(s) 367
BspLI GGNNCC 3 cut(s) 567, 694, 717
BspMAI CTGCAG 2 cut(s) 262, 616
BsrDI GCAATG 2 cut(s) 365, 508
BsrI ACTGG 1 cut(s) 144
BssECI CCNNGG 3 cut(s) 375, 449, 655
BssMI GATC 1 cut(s) 630
BssT1I CCWWGG 1 cut(s) 375
Bst4CI ACNGT 2 cut(s) 141, 656
Bst6I CTCTTC 2 cut(s) 34, 516
BstAPI GCANNNNNTGC 2 cut(s) 266, 611
BstC8I GCNNGC 3 cut(s) 241, 255, 271
BstDEI CTNAG 1 cut(s) 627
BstDSI CCRYGG 1 cut(s) 655
BstF5I GGATG 1 cut(s) 13
BstHHI GCGC 1 cut(s) 543
BstKTI GATC 1 cut(s) 633
BstMAI GTCTC 1 cut(s) 402
BstMBI GATC 1 cut(s) 630
BstMWI GCNNNNNNNGC 4 cut(s) 119, 266, 608, 611
BstNSI RCATGY 2 cut(s) 117, 501
BstSCI CCNGG 2 cut(s) 448, 449
BstSFI CTRYAG 2 cut(s) 258, 612
BstV1I GCAGC 6 cut(s) 97, 244, 272, 564, 589, 598
BsuRI GGCC 4 cut(s) 338, 442, 485, 716
BtgI CCRYGG 1 cut(s) 655
BtsCI GGATG 1 cut(s) 13
BtsI GCAGTG 1 cut(s) 643
BtsIMutI CAGTG 2 cut(s) 151, 643
Cac8I GCNNGC 3 cut(s) 241, 255, 271
CciI TCATGA 1 cut(s) 367
CfoI GCGC 1 cut(s) 543
Cfr13I GGNCC 3 cut(s) 440, 484, 715
Cfr9I CCCGGG 1 cut(s) 449
Csp6I GTAC 2 cut(s) 213, 621
CviAII CATG 5 cut(s) 114, 200, 368, 498, 544
CviQI GTAC 2 cut(s) 213, 621
DdeI CTNAG 1 cut(s) 627
DpnI GATC 1 cut(s) 632
DpnII GATC 1 cut(s) 630
EaeI YGGCCR 1 cut(s) 336
Eam1104I CTCTTC 2 cut(s) 34, 516
EarI CTCTTC 2 cut(s) 34, 516
Eco130I CCWWGG 1 cut(s) 375
Eco32I GATATC 1 cut(s) 700
Eco88I CYCGRG 1 cut(s) 449
EcoRV GATATC 1 cut(s) 700
EcoT14I CCWWGG 1 cut(s) 375
EcoT22I ATGCAT 1 cut(s) 31
ErhI CCWWGG 1 cut(s) 375
FaeI CATG 5 cut(s) 117, 203, 371, 501, 547
FatI CATG 5 cut(s) 113, 199, 367, 497, 543
FauNDI CATATG 2 cut(s) 25, 684
Fnu4HI GCNGC 6 cut(s) 111, 258, 261, 578, 603, 612
FokI GGATG 1 cut(s) 20
Fsp4HI GCNGC 6 cut(s) 111, 258, 261, 578, 603, 612
FspBI CTAG 2 cut(s) 78, 730
GlaI GCGC 1 cut(s) 542
GluI GCNGC 6 cut(s) 111, 258, 261, 578, 603, 612
GsaI CCCAGC 1 cut(s) 692
HaeIII GGCC 4 cut(s) 338, 442, 485, 716
HapII CCGG 2 cut(s) 450, 515
HhaI GCGC 1 cut(s) 543
Hin1II CATG 5 cut(s) 117, 203, 371, 501, 547
Hin6I GCGC 1 cut(s) 541
HinP1I GCGC 1 cut(s) 541
HindIII AAGCTT 1 cut(s) 170
HinfI GANTC 1 cut(s) 524
HpaII CCGG 2 cut(s) 450, 515
HphI GGTGA 2 cut(s) 282, 419
Hpy188I TCNGA 3 cut(s) 105, 321, 391
Hpy188III TCNNGA 1 cut(s) 368
HpyAV CCTTC 3 cut(s) 110, 312, 611
HpyCH4III ACNGT 2 cut(s) 141, 656
HpyCH4IV ACGT 1 cut(s) 245
HpyF10VI GCNNNNNNNGC 4 cut(s) 119, 266, 608, 611
HpyF3I CTNAG 1 cut(s) 627
HpySE526I ACGT 1 cut(s) 245
Hsp92II CATG 5 cut(s) 117, 203, 371, 501, 547
HspAI GCGC 1 cut(s) 541
Kzo9I GATC 1 cut(s) 630
LmnI GCTCC 1 cut(s) 692
Lsp1109I GCAGC 6 cut(s) 97, 244, 272, 564, 589, 598
LweI GCATC 1 cut(s) 312
MaeI CTAG 2 cut(s) 78, 730
MaeII ACGT 1 cut(s) 245
MalI GATC 1 cut(s) 632
MboI GATC 1 cut(s) 630
MboII GAAGA 3 cut(s) 51, 533, 717
MlsI TGGCCA 1 cut(s) 338
MluCI AATT 3 cut(s) 70, 285, 412
MluNI TGGCCA 1 cut(s) 338
MlyI GAGTC 1 cut(s) 533
MmeI TCCRAC 2 cut(s) 88, 493
MnlI CCTC 5 cut(s) 35, 413, 421, 483, 598
Mox20I TGGCCA 1 cut(s) 338
Mph1103I ATGCAT 1 cut(s) 31
MroXI GAANNNNTTC 1 cut(s) 99
MscI TGGCCA 1 cut(s) 338
MseI TTAA 5 cut(s) 32, 69, 432, 509, 710
MslI CAYNNNNRTG 1 cut(s) 198
Msp20I TGGCCA 1 cut(s) 338
MspI CCGG 2 cut(s) 450, 515
MspR9I CCNGG 2 cut(s) 450, 451
MwoI GCNNNNNNNGC 4 cut(s) 119, 266, 608, 611
NciI CCSGG 2 cut(s) 450, 451
NdeI CATATG 2 cut(s) 25, 684
NdeII GATC 1 cut(s) 630
NlaIII CATG 5 cut(s) 117, 203, 371, 501, 547
NlaIV GGNNCC 3 cut(s) 567, 694, 717
NsiI ATGCAT 1 cut(s) 31
NspI RCATGY 2 cut(s) 117, 501
PagI TCATGA 1 cut(s) 367
PdmI GAANNNNTTC 1 cut(s) 99
PkrI GCNGC 6 cut(s) 112, 259, 262, 579, 604, 613
PleI GAGTC 1 cut(s) 532
PpsI GAGTC 1 cut(s) 532
PshBI ATTAAT 1 cut(s) 710
PspFI CCCAGC 1 cut(s) 688
PspN4I GGNNCC 3 cut(s) 567, 694, 717
PspPI GGNCC 3 cut(s) 440, 484, 715
PstI CTGCAG 2 cut(s) 262, 616
RsaI GTAC 2 cut(s) 214, 622
RsaNI GTAC 2 cut(s) 213, 621
RseI CAYNNNNRTG 1 cut(s) 198
SaqAI TTAA 5 cut(s) 32, 69, 432, 509, 710
SatI GCNGC 6 cut(s) 111, 258, 261, 578, 603, 612
Sau3AI GATC 1 cut(s) 630
Sau96I GGNCC 3 cut(s) 440, 484, 715
SchI GAGTC 1 cut(s) 533
ScrFI CCNGG 2 cut(s) 450, 451
SfaNI GCATC 1 cut(s) 312
SfcI CTRYAG 2 cut(s) 258, 612
SmaI CCCGGG 1 cut(s) 451
SmiMI CAYNNNNRTG 1 cut(s) 198
Sse9I AATT 3 cut(s) 70, 285, 412
SspMI CTAG 2 cut(s) 78, 730
StyD4I CCNGG 2 cut(s) 448, 449
StyI CCWWGG 1 cut(s) 375
TaaI ACNGT 2 cut(s) 141, 656
TaiI ACGT 1 cut(s) 248
TaqI TCGA 1 cut(s) 475
TasI AATT 3 cut(s) 70, 285, 412
TatI WGTACW 1 cut(s) 212
Tru1I TTAA 5 cut(s) 32, 69, 432, 509, 710
Tru9I TTAA 5 cut(s) 32, 69, 432, 509, 710
TscAI CASTG 2 cut(s) 151, 643
TseI GCWGC 6 cut(s) 110, 257, 260, 577, 602, 611
TspDTI ATGAA 4 cut(s) 17, 188, 268, 671
TspMI CCCGGG 1 cut(s) 449
TspRI CASTG 2 cut(s) 151, 643
VspI ATTAAT 1 cut(s) 710
XapI RAATTY 1 cut(s) 412
XceI RCATGY 2 cut(s) 117, 501
XmaI CCCGGG 1 cut(s) 449
XmnI GAANNNNTTC 1 cut(s) 99
XspI CTAG 2 cut(s) 78, 730
Zsp2I ATGCAT 1 cut(s) 31
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.