Rh7AG059300

EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0001544
Physical Location & Seq
Reverse (-)
4096795 .. 4100980
4186 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG059300.1

Sequence Viewer

Length: 420 bp
ATGGCTGCATGTATGGCTGATGGAACAACTATACTGTCCAATGTTGCCAGAGAACCAGAGGTGGTTGATCTTGCTCGATTTCTGACTAAAAATGGGGCCTGTGTGGAAGGAGCAGGAAGCAACAAGCTTGTCATCAAGGGAAAATCTTATTTGCATGGTTGTAAATGTACTATTGCACCTGATCGTATTGAAGCAGGCACATTTATGCTTGCTGCAGCTATTACTCGCTCATTCATTTCAATTTCACCTGTCATTCCTTCCCAAGTTTCCTGTCTGATGCAAAAACTCTTGGCCGCTGGTTGCAAAATAAGGTTTCAGCAGTGTCTCAATGTGGTGAAAATTTGCGAGGTTTTGAGGTTAAGACAGGGCCATTTCCCGGGTTTCCTACAGATCTTCAACTGCAAACAATGGCACTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPSP_synthase

Protein Analysis

139

Amino Acids

15.13

Weight (kDa)

9.14

Isoelectric Point (pI)

47.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPSP_synthase PF00275 1 - 105 1.7e-21 EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 294
AcoI YGGCCR 1 cut(s) 291
AcsI RAATTY 1 cut(s) 339
AfaI GTAC 1 cut(s) 169
AfiI CCNNNNNNNGG 1 cut(s) 376
AgsI TTSAA 3 cut(s) 191, 240, 397
AluBI AGCT 2 cut(s) 127, 218
AluI AGCT 2 cut(s) 127, 218
Alw26I GTCTC 1 cut(s) 329
Ama87I CYCGRG 1 cut(s) 376
AoxI GGCC 3 cut(s) 96, 291, 367
ApeKI GCWGC 3 cut(s) 5, 212, 215
ApoI RAATTY 1 cut(s) 339
AspS9I GGNCC 2 cut(s) 96, 367
AsuC2I CCSGG 2 cut(s) 377, 378
AsuHPI GGTGA 2 cut(s) 237, 346
AvaI CYCGRG 1 cut(s) 376
BbvI GCAGC 2 cut(s) 199, 227
BccI CCATC 1 cut(s) 14
BcnI CCSGG 2 cut(s) 377, 378
BcoDI GTCTC 1 cut(s) 329
BfmI CTRYAG 2 cut(s) 213, 386
BglII AGATCT 1 cut(s) 390
BisI GCNGC 4 cut(s) 6, 213, 216, 294
BlsI GCNGC 4 cut(s) 7, 214, 217, 295
Bme1390I CCNGG 2 cut(s) 377, 378
BmeT110I CYCGRG 1 cut(s) 376
BmgT120I GGNCC 2 cut(s) 96, 367
BmiI GGNNCC 1 cut(s) 97
BmrFI CCNGG 2 cut(s) 377, 378
BmsI GCATC 1 cut(s) 267
BpuMI CCSGG 2 cut(s) 377, 378
BsaJI CCNNGG 1 cut(s) 376
Bsc4I CCNNNNNNNGG 1 cut(s) 376
BseDI CCNNGG 1 cut(s) 376
BseLI CCNNNNNNNGG 1 cut(s) 376
BseXI GCAGC 2 cut(s) 199, 227
BshFI GGCC 3 cut(s) 98, 293, 369
BsiHKCI CYCGRG 1 cut(s) 376
BsiSI CCGG 1 cut(s) 377
BslI CCNNNNNNNGG 1 cut(s) 376
BsmAI GTCTC 1 cut(s) 329
BsnI GGCC 3 cut(s) 98, 293, 369
BsoBI CYCGRG 1 cut(s) 376
Bsp143I GATC 3 cut(s) 67, 181, 390
BspACI CCGC 1 cut(s) 294
BspANI GGCC 3 cut(s) 98, 293, 369
BspLI GGNNCC 1 cut(s) 97
BspMAI CTGCAG 1 cut(s) 217
BssECI CCNNGG 1 cut(s) 376
BssMI GATC 3 cut(s) 67, 181, 390
Bst4CI ACNGT 1 cut(s) 36
BstC8I GCNNGC 2 cut(s) 196, 210
BstKTI GATC 3 cut(s) 70, 184, 393
BstMAI GTCTC 1 cut(s) 329
BstMBI GATC 3 cut(s) 67, 181, 390
BstMWI GCNNNNNNNGC 1 cut(s) 14
BstNSI RCATGY 1 cut(s) 12
BstSCI CCNGG 2 cut(s) 375, 376
BstSFI CTRYAG 2 cut(s) 213, 386
BstV1I GCAGC 2 cut(s) 199, 227
BstX2I RGATCY 1 cut(s) 390
BstYI RGATCY 1 cut(s) 390
BsuRI GGCC 3 cut(s) 98, 293, 369
BtsI GCAGTG 2 cut(s) 326, 412
BtsIMutI CAGTG 2 cut(s) 326, 412
Cac8I GCNNGC 2 cut(s) 196, 210
Cfr13I GGNCC 2 cut(s) 96, 367
Cfr9I CCCGGG 1 cut(s) 376
Csp6I GTAC 1 cut(s) 168
CviAII CATG 2 cut(s) 9, 155
CviJI RGCY 7 cut(s) 5, 17, 98, 127, 218, 293, 369
CviKI_1 RGCY 7 cut(s) 5, 17, 98, 127, 218, 293, 369
CviQI GTAC 1 cut(s) 168
DpnI GATC 3 cut(s) 69, 183, 392
DpnII GATC 3 cut(s) 67, 181, 390
EaeI YGGCCR 1 cut(s) 291
Eco88I CYCGRG 1 cut(s) 376
EcoO109I RGGNCCY 1 cut(s) 96
FaeI CATG 2 cut(s) 12, 158
FaiI YATR 5 cut(s) 10, 14, 32, 156, 206
FatI CATG 2 cut(s) 8, 154
Fnu4HI GCNGC 4 cut(s) 6, 213, 216, 294
Fsp4HI GCNGC 4 cut(s) 6, 213, 216, 294
GluI GCNGC 4 cut(s) 6, 213, 216, 294
HaeIII GGCC 3 cut(s) 98, 293, 369
HapII CCGG 1 cut(s) 377
Hin1II CATG 2 cut(s) 12, 158
HindIII AAGCTT 1 cut(s) 125
HpaII CCGG 1 cut(s) 377
HphI GGTGA 2 cut(s) 237, 346
Hpy188I TCNGA 2 cut(s) 84, 276
HpyAV CCTTC 2 cut(s) 101, 267
HpyCH4III ACNGT 1 cut(s) 36
HpyCH4V TGCA 7 cut(s) 8, 154, 176, 215, 280, 303, 402
HpyF10VI GCNNNNNNNGC 1 cut(s) 14
Hsp92II CATG 2 cut(s) 12, 158
Kzo9I GATC 3 cut(s) 67, 181, 390
LmnI GCTCC 1 cut(s) 110
Lsp1109I GCAGC 2 cut(s) 199, 227
LweI GCATC 1 cut(s) 267
MalI GATC 3 cut(s) 69, 183, 392
MboI GATC 3 cut(s) 67, 181, 390
MboII GAAGA 1 cut(s) 385
MflI RGATCY 1 cut(s) 390
MluCI AATT 2 cut(s) 240, 339
MnlI CCTC 3 cut(s) 52, 340, 348
MseI TTAA 1 cut(s) 359
MslI CAYNNNNRTG 1 cut(s) 203
MspA1I CMGCKG 1 cut(s) 296
MspI CCGG 1 cut(s) 377
MspR9I CCNGG 2 cut(s) 377, 378
MwoI GCNNNNNNNGC 1 cut(s) 14
NciI CCSGG 2 cut(s) 377, 378
NdeII GATC 3 cut(s) 67, 181, 390
NlaIII CATG 2 cut(s) 12, 158
NlaIV GGNNCC 1 cut(s) 97
NspI RCATGY 1 cut(s) 12
PkrI GCNGC 4 cut(s) 7, 214, 217, 295
PspN4I GGNNCC 1 cut(s) 97
PspPI GGNCC 2 cut(s) 96, 367
PstI CTGCAG 1 cut(s) 217
PsuI RGATCY 1 cut(s) 390
RsaI GTAC 1 cut(s) 169
RsaNI GTAC 1 cut(s) 168
RseI CAYNNNNRTG 1 cut(s) 203
SaqAI TTAA 1 cut(s) 359
SatI GCNGC 4 cut(s) 6, 213, 216, 294
Sau3AI GATC 3 cut(s) 67, 181, 390
Sau96I GGNCC 2 cut(s) 96, 367
ScrFI CCNGG 2 cut(s) 377, 378
SetI ASST 8 cut(s) 63, 129, 181, 220, 250, 314, 351, 359
SfaNI GCATC 1 cut(s) 267
SfcI CTRYAG 2 cut(s) 213, 386
SmaI CCCGGG 1 cut(s) 378
SmiMI CAYNNNNRTG 1 cut(s) 203
Sse9I AATT 2 cut(s) 240, 339
SsiI CCGC 1 cut(s) 294
StyD4I CCNGG 2 cut(s) 375, 376
TaaI ACNGT 1 cut(s) 36
TaqI TCGA 1 cut(s) 76
TasI AATT 2 cut(s) 240, 339
TatI WGTACW 1 cut(s) 167
TauI GCSGC 1 cut(s) 296
Tru1I TTAA 1 cut(s) 359
Tru9I TTAA 1 cut(s) 359
TscAI CASTG 2 cut(s) 326, 419
TseI GCWGC 3 cut(s) 5, 212, 215
TspDTI ATGAA 1 cut(s) 223
TspMI CCCGGG 1 cut(s) 376
TspRI CASTG 2 cut(s) 326, 419
XapI RAATTY 1 cut(s) 339
XceI RCATGY 1 cut(s) 12
XmaI CCCGGG 1 cut(s) 376
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.