RLG00000009452

EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0001544
Physical Location & Seq
Reverse (-)
53050468 .. 53052177
1710 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000009452

Sequence Viewer

Length: 753 bp
ATGGCAGCTTTGGCAAATTCGGATGGCAAAGTTCAGGCATATGCATTAAATGGAAGAGGACTAGTTGGGGGAAGTTTCCAACTTGATTACCCTAGCATTGGGGCAACAAAAACCCTTCTAATGGCTGCATGTATGGCTGATGGAACAACTATACAATCCAATGTTGCCAGAGAACCAGAGGTGGTTGATCTTGCTCGATTTCTGACTAACAGTGGGGCCTTTGTGGAAGGAGCAGGAAGCAACAAGCTTGTCATCAAGGGAAAATCTCATTTGCATGGTTGTAAATGTACTATTGCACCTGATCGTATTGAAGCAGGCACATTTATGCTTGCTGCAGCTATTACTCGCTCATTCATTTCAATTTCACCTGTCATTCCTTCCCAAATTTCCTGTCTGAAACAAAAACTCTTGGCCGCGGGTTGCAAAATAAGGCAATGCTGTCATGATACCGTGGAAGTTTCAGCAGTGTCTCGCTGTGGTGAGAATTTGCGAGGTTTTGAGGTTAAGACAGGGCCATTTCCCGGGTTTCCTACGGATCTTCAACCGCAAACAATGGCACTGCTAAGCACTTGCTTTATACTAATAAACGAACTGCGGAAGCTTGGAGCAAAAATTCTAGTCTCTGCAAGCTCTGCTCTGGTTTTCGGGAAAGATAATAGAAATGGTTTGTCTGGTTCCTGCCTTGCTGCAACTGACCTCAGAGGTGGGGATATCATTGGTATTAGCTGCATTGGCTGCAGAAGGTACTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPSP_synthase

Protein Analysis

251

Amino Acids

26.27

Weight (kDa)

8.95

Isoelectric Point (pI)

41.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPSP_synthase PF00275 17 - 190 4.7e-33 EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 416
AciI CCGC 4 cut(s) 414, 416, 545, 595
AclWI GGATC 1 cut(s) 543
AcoI YGGCCR 1 cut(s) 411
AcsI RAATTY 4 cut(s) 16, 384, 484, 612
AfaI GTAC 2 cut(s) 289, 746
AfiI CCNNNNNNNGG 3 cut(s) 98, 121, 521
AgsI TTSAA 3 cut(s) 311, 360, 542
AhlI ACTAGT 1 cut(s) 61
AluBI AGCT 6 cut(s) 8, 247, 338, 601, 630, 726
AluI AGCT 6 cut(s) 8, 247, 338, 601, 630, 726
Alw26I GTCTC 2 cut(s) 474, 625
AlwI GGATC 1 cut(s) 543
Ama87I CYCGRG 1 cut(s) 521
AoxI GGCC 3 cut(s) 216, 411, 512
ApeKI GCWGC 7 cut(s) 5, 125, 332, 335, 686, 726, 735
ApoI RAATTY 4 cut(s) 16, 384, 484, 612
ArsI GACNNNNNNTTYG 2 cut(s) 376, 408
AspS9I GGNCC 2 cut(s) 216, 512
AsuC2I CCSGG 2 cut(s) 522, 523
AsuHPI GGTGA 2 cut(s) 357, 491
AvaI CYCGRG 1 cut(s) 521
BbvI GCAGC 7 cut(s) 17, 112, 319, 347, 673, 713, 722
BccI CCATC 2 cut(s) 17, 134
BcnI CCSGG 2 cut(s) 522, 523
BcoDI GTCTC 2 cut(s) 474, 625
BcuI ACTAGT 1 cut(s) 61
BfaI CTAG 3 cut(s) 62, 93, 617
BfmI CTRYAG 2 cut(s) 333, 736
BisI GCNGC 8 cut(s) 6, 126, 333, 336, 414, 687, 727, 736
BlpI GCTNAGC 1 cut(s) 563
BlsI GCNGC 8 cut(s) 7, 127, 334, 337, 415, 688, 728, 737
Bme1390I CCNGG 2 cut(s) 522, 523
BmeT110I CYCGRG 1 cut(s) 521
BmgT120I GGNCC 2 cut(s) 216, 512
BmiI GGNNCC 2 cut(s) 217, 676
BmrFI CCNGG 2 cut(s) 522, 523
Bpu1102I GCTNAGC 1 cut(s) 563
BpuMI CCSGG 2 cut(s) 522, 523
BsaJI CCNNGG 3 cut(s) 414, 450, 521
Bsc4I CCNNNNNNNGG 3 cut(s) 98, 121, 521
Bse3DI GCAATG 1 cut(s) 440
BseDI CCNNGG 3 cut(s) 414, 450, 521
BseGI GGATG 1 cut(s) 28
BseLI CCNNNNNNNGG 3 cut(s) 98, 121, 521
BseMI GCAATG 1 cut(s) 440
BseMII CTCAG 1 cut(s) 712
BseXI GCAGC 7 cut(s) 17, 112, 319, 347, 673, 713, 722
Bsh1236I CGCG 1 cut(s) 416
BshFI GGCC 3 cut(s) 218, 413, 514
BsiHKCI CYCGRG 1 cut(s) 521
BsiSI CCGG 1 cut(s) 522
BslI CCNNNNNNNGG 3 cut(s) 98, 121, 521
BsmAI GTCTC 2 cut(s) 474, 625
BsnI GGCC 3 cut(s) 218, 413, 514
BsoBI CYCGRG 1 cut(s) 521
Bsp143I GATC 3 cut(s) 187, 301, 535
Bsp1720I GCTNAGC 1 cut(s) 563
BspACI CCGC 4 cut(s) 414, 416, 545, 595
BspANI GGCC 3 cut(s) 218, 413, 514
BspCNI CTCAG 1 cut(s) 711
BspFNI CGCG 1 cut(s) 416
BspHI TCATGA 1 cut(s) 442
BspLI GGNNCC 2 cut(s) 217, 676
BspMAI CTGCAG 2 cut(s) 337, 740
BspPI GGATC 1 cut(s) 543
BsrDI GCAATG 1 cut(s) 440
BssECI CCNNGG 3 cut(s) 414, 450, 521
BssMI GATC 3 cut(s) 187, 301, 535
Bst4CI ACNGT 2 cut(s) 212, 451
Bst6I CTCTTC 1 cut(s) 49
BstAPI GCANNNNNTGC 2 cut(s) 632, 735
BstC8I GCNNGC 3 cut(s) 316, 330, 628
BstDEI CTNAG 2 cut(s) 563, 698
BstDSI CCRYGG 2 cut(s) 414, 450
BstF5I GGATG 1 cut(s) 28
BstFNI CGCG 1 cut(s) 416
BstKTI GATC 3 cut(s) 190, 304, 538
BstMAI GTCTC 2 cut(s) 474, 625
BstMBI GATC 3 cut(s) 187, 301, 535
BstMWI GCNNNNNNNGC 5 cut(s) 11, 134, 632, 732, 735
BstNSI RCATGY 1 cut(s) 132
BstSCI CCNGG 2 cut(s) 520, 521
BstSFI CTRYAG 2 cut(s) 333, 736
BstUI CGCG 1 cut(s) 416
BstV1I GCAGC 7 cut(s) 17, 112, 319, 347, 673, 713, 722
BstX2I RGATCY 1 cut(s) 535
BstYI RGATCY 1 cut(s) 535
BsuRI GGCC 3 cut(s) 218, 413, 514
BtgI CCRYGG 2 cut(s) 414, 450
BtsCI GGATG 1 cut(s) 28
BtsI GCAGTG 2 cut(s) 471, 557
BtsIMutI CAGTG 3 cut(s) 217, 471, 557
Cac8I GCNNGC 3 cut(s) 316, 330, 628
CciI TCATGA 1 cut(s) 442
Cfr13I GGNCC 2 cut(s) 216, 512
Cfr42I CCGCGG 1 cut(s) 417
Cfr9I CCCGGG 1 cut(s) 521
Csp6I GTAC 2 cut(s) 288, 745
CviAII CATG 3 cut(s) 129, 275, 443
CviQI GTAC 2 cut(s) 288, 745
DdeI CTNAG 2 cut(s) 563, 698
DpnI GATC 3 cut(s) 189, 303, 537
DpnII GATC 3 cut(s) 187, 301, 535
EaeI YGGCCR 1 cut(s) 411
Eam1104I CTCTTC 1 cut(s) 49
EarI CTCTTC 1 cut(s) 49
Eco32I GATATC 1 cut(s) 712
Eco88I CYCGRG 1 cut(s) 521
EcoO109I RGGNCCY 1 cut(s) 216
EcoRV GATATC 1 cut(s) 712
EcoT22I ATGCAT 1 cut(s) 46
FaeI CATG 3 cut(s) 132, 278, 446
FaiI YATR 9 cut(s) 40, 42, 130, 134, 152, 276, 326, 444, 578
FatI CATG 3 cut(s) 128, 274, 442
FauI CCCGC 1 cut(s) 409
FauNDI CATATG 1 cut(s) 40
Fnu4HI GCNGC 8 cut(s) 6, 126, 333, 336, 414, 687, 727, 736
FokI GGATG 1 cut(s) 35
Fsp4HI GCNGC 8 cut(s) 6, 126, 333, 336, 414, 687, 727, 736
FspBI CTAG 3 cut(s) 62, 93, 617
GluI GCNGC 8 cut(s) 6, 126, 333, 336, 414, 687, 727, 736
HaeIII GGCC 3 cut(s) 218, 413, 514
HapII CCGG 1 cut(s) 522
Hin1II CATG 3 cut(s) 132, 278, 446
HindIII AAGCTT 2 cut(s) 245, 599
HpaII CCGG 1 cut(s) 522
HphI GGTGA 2 cut(s) 357, 491
Hpy188I TCNGA 4 cut(s) 22, 204, 396, 701
Hpy188III TCNNGA 2 cut(s) 443, 646
HpyAV CCTTC 4 cut(s) 125, 221, 387, 735
HpyCH4III ACNGT 2 cut(s) 212, 451
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 134, 632, 732, 735
HpyF3I CTNAG 2 cut(s) 563, 698
Hsp92II CATG 3 cut(s) 132, 278, 446
KspI CCGCGG 1 cut(s) 417
Kzo9I GATC 3 cut(s) 187, 301, 535
LmnI GCTCC 2 cut(s) 230, 605
Lsp1109I GCAGC 7 cut(s) 17, 112, 319, 347, 673, 713, 722
MaeI CTAG 3 cut(s) 62, 93, 617
MalI GATC 3 cut(s) 189, 303, 537
MboI GATC 3 cut(s) 187, 301, 535
MboII GAAGA 2 cut(s) 66, 530
MflI RGATCY 1 cut(s) 535
MluCI AATT 5 cut(s) 16, 360, 384, 484, 612
MmeI TCCRAC 1 cut(s) 103
MnlI CCTC 6 cut(s) 50, 172, 485, 493, 695, 707
Mph1103I ATGCAT 1 cut(s) 46
MseI TTAA 2 cut(s) 47, 504
MslI CAYNNNNRTG 2 cut(s) 273, 323
MspA1I CMGCKG 1 cut(s) 416
MspI CCGG 1 cut(s) 522
MspR9I CCNGG 2 cut(s) 522, 523
MvnI CGCG 1 cut(s) 416
MwoI GCNNNNNNNGC 5 cut(s) 11, 134, 632, 732, 735
NciI CCSGG 2 cut(s) 522, 523
NdeI CATATG 1 cut(s) 40
NdeII GATC 3 cut(s) 187, 301, 535
NlaIII CATG 3 cut(s) 132, 278, 446
NlaIV GGNNCC 2 cut(s) 217, 676
NsiI ATGCAT 1 cut(s) 46
NspI RCATGY 1 cut(s) 132
PagI TCATGA 1 cut(s) 442
PkrI GCNGC 8 cut(s) 7, 127, 334, 337, 415, 688, 728, 737
PspN4I GGNNCC 2 cut(s) 217, 676
PspPI GGNCC 2 cut(s) 216, 512
PstI CTGCAG 2 cut(s) 337, 740
PsuI RGATCY 1 cut(s) 535
RsaI GTAC 2 cut(s) 289, 746
RsaNI GTAC 2 cut(s) 288, 745
RseI CAYNNNNRTG 2 cut(s) 273, 323
SacII CCGCGG 1 cut(s) 417
SaqAI TTAA 2 cut(s) 47, 504
SatI GCNGC 8 cut(s) 6, 126, 333, 336, 414, 687, 727, 736
Sau3AI GATC 3 cut(s) 187, 301, 535
Sau96I GGNCC 2 cut(s) 216, 512
ScrFI CCNGG 2 cut(s) 522, 523
SfcI CTRYAG 2 cut(s) 333, 736
Sfr303I CCGCGG 1 cut(s) 417
SgrBI CCGCGG 1 cut(s) 417
SmaI CCCGGG 1 cut(s) 523
SmiMI CAYNNNNRTG 2 cut(s) 273, 323
SpeI ACTAGT 1 cut(s) 61
Sse9I AATT 5 cut(s) 16, 360, 384, 484, 612
SsiI CCGC 4 cut(s) 414, 416, 545, 595
SspMI CTAG 3 cut(s) 62, 93, 617
StyD4I CCNGG 2 cut(s) 520, 521
TaaI ACNGT 2 cut(s) 212, 451
TaqI TCGA 1 cut(s) 196
TasI AATT 5 cut(s) 16, 360, 384, 484, 612
TatI WGTACW 1 cut(s) 287
TauI GCSGC 1 cut(s) 416
Tru1I TTAA 2 cut(s) 47, 504
Tru9I TTAA 2 cut(s) 47, 504
TscAI CASTG 3 cut(s) 217, 471, 564
TseI GCWGC 7 cut(s) 5, 125, 332, 335, 686, 726, 735
TspDTI ATGAA 1 cut(s) 343
TspGWI ACGGA 1 cut(s) 548
TspMI CCCGGG 1 cut(s) 521
TspRI CASTG 3 cut(s) 217, 471, 564
XapI RAATTY 4 cut(s) 16, 384, 484, 612
XceI RCATGY 1 cut(s) 132
XmaI CCCGGG 1 cut(s) 521
XspI CTAG 3 cut(s) 62, 93, 617
Zsp2I ATGCAT 1 cut(s) 46
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.