RLG00000029419

EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0001544
Physical Location & Seq
Forward (+)
38477388 .. 38478082
695 bp
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UTR
Exon/CDS
Intron
RLM00000029419

Sequence Viewer

Length: 456 bp
ATGGCTGCATGTATGGCTGATGGAACAACTATACTGTCCAATGCTGCCAGAGAACCAGAGGTGGTTGATCTTGCTCGATTTCTGACTAAAAGTGGGGCATGTGTGGAGGGAGCAGGAAGCAACAAGCTTGTCATCAAGGGAAAATCTCATTTCCATGGTTGTAAGTGTACTATTGCACCTGATCGTATTGAAGCAGGCACATTTATGCTTGCTGCAGCTATTACTCGCTCATTCATTTCAATTTCGCCTGTCATTCCTTTCCAAGTTTCCTGTCTGATGCAAAAACTCTTGGCCGCTGGTTGCAAAATAAGGCAATGCTCTCATGATACCTTGGAAGTTTCGGCAGTGTCTCGATGTGGTGAGAATTTGCGAGGTTTTGAGGTTAAGACGGCCATTTCCGGGGTTTCCTACAGATCTCCAACCGCAAACAATGGCACTGCTAACCACTTGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPSP_synthase

Protein Analysis

152

Amino Acids

15.89

Weight (kDa)

8.87

Isoelectric Point (pI)

36.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPSP_synthase PF00275 1 - 132 3e-23 EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 294, 423
AcoI YGGCCR 2 cut(s) 291, 390
AcsI RAATTY 1 cut(s) 364
AfaI GTAC 1 cut(s) 169
AfiI CCNNNNNNNGG 1 cut(s) 399
AgsI TTSAA 2 cut(s) 191, 240
AluBI AGCT 2 cut(s) 127, 218
AluI AGCT 2 cut(s) 127, 218
Alw26I GTCTC 1 cut(s) 354
AoxI GGCC 2 cut(s) 291, 390
ApeKI GCWGC 4 cut(s) 5, 44, 212, 215
ApoI RAATTY 1 cut(s) 364
AsuC2I CCSGG 1 cut(s) 400
AsuHPI GGTGA 1 cut(s) 371
BbvI GCAGC 3 cut(s) 31, 199, 227
BccI CCATC 1 cut(s) 14
BceAI ACGGC 1 cut(s) 405
BcnI CCSGG 1 cut(s) 400
BcoDI GTCTC 1 cut(s) 354
BfmI CTRYAG 2 cut(s) 213, 409
BglII AGATCT 1 cut(s) 413
BisI GCNGC 5 cut(s) 6, 45, 213, 216, 294
BlsI GCNGC 5 cut(s) 7, 46, 214, 217, 295
Bme1390I CCNGG 1 cut(s) 400
BmrFI CCNGG 1 cut(s) 400
BmsI GCATC 1 cut(s) 267
BpuMI CCSGG 1 cut(s) 400
BsaJI CCNNGG 3 cut(s) 154, 330, 399
Bsc4I CCNNNNNNNGG 1 cut(s) 399
Bse3DI GCAATG 1 cut(s) 320
BseDI CCNNGG 3 cut(s) 154, 330, 399
BseLI CCNNNNNNNGG 1 cut(s) 399
BseMI GCAATG 1 cut(s) 320
BseXI GCAGC 3 cut(s) 31, 199, 227
BshFI GGCC 2 cut(s) 293, 392
BsiSI CCGG 1 cut(s) 399
BslI CCNNNNNNNGG 1 cut(s) 399
BsmAI GTCTC 1 cut(s) 354
BsnI GGCC 2 cut(s) 293, 392
Bsp143I GATC 3 cut(s) 67, 181, 413
Bsp19I CCATGG 1 cut(s) 154
BspACI CCGC 2 cut(s) 294, 423
BspANI GGCC 2 cut(s) 293, 392
BspHI TCATGA 1 cut(s) 322
BspMAI CTGCAG 1 cut(s) 217
BsrDI GCAATG 1 cut(s) 320
BssECI CCNNGG 3 cut(s) 154, 330, 399
BssMI GATC 3 cut(s) 67, 181, 413
BssT1I CCWWGG 2 cut(s) 154, 330
Bst4CI ACNGT 1 cut(s) 36
BstC8I GCNNGC 2 cut(s) 196, 210
BstDSI CCRYGG 1 cut(s) 154
BstKTI GATC 3 cut(s) 70, 184, 416
BstMAI GTCTC 1 cut(s) 354
BstMBI GATC 3 cut(s) 67, 181, 413
BstMWI GCNNNNNNNGC 1 cut(s) 14
BstNSI RCATGY 2 cut(s) 12, 102
BstSCI CCNGG 1 cut(s) 398
BstSFI CTRYAG 2 cut(s) 213, 409
BstV1I GCAGC 3 cut(s) 31, 199, 227
BstX2I RGATCY 1 cut(s) 413
BstYI RGATCY 1 cut(s) 413
BsuRI GGCC 2 cut(s) 293, 392
BtgI CCRYGG 1 cut(s) 154
BtsI GCAGTG 2 cut(s) 351, 435
BtsIMutI CAGTG 2 cut(s) 351, 435
Cac8I GCNNGC 2 cut(s) 196, 210
CciI TCATGA 1 cut(s) 322
Csp6I GTAC 1 cut(s) 168
CviAII CATG 4 cut(s) 9, 99, 155, 323
CviJI RGCY 6 cut(s) 5, 17, 127, 218, 293, 392
CviKI_1 RGCY 6 cut(s) 5, 17, 127, 218, 293, 392
CviQI GTAC 1 cut(s) 168
DpnI GATC 3 cut(s) 69, 183, 415
DpnII GATC 3 cut(s) 67, 181, 413
EaeI YGGCCR 2 cut(s) 291, 390
Eco130I CCWWGG 2 cut(s) 154, 330
EcoT14I CCWWGG 2 cut(s) 154, 330
ErhI CCWWGG 2 cut(s) 154, 330
FaeI CATG 4 cut(s) 12, 102, 158, 326
FaiI YATR 7 cut(s) 10, 14, 32, 100, 156, 206, 324
FatI CATG 4 cut(s) 8, 98, 154, 322
Fnu4HI GCNGC 5 cut(s) 6, 45, 213, 216, 294
Fsp4HI GCNGC 5 cut(s) 6, 45, 213, 216, 294
GluI GCNGC 5 cut(s) 6, 45, 213, 216, 294
HaeIII GGCC 2 cut(s) 293, 392
HapII CCGG 1 cut(s) 399
Hin1II CATG 4 cut(s) 12, 102, 158, 326
HindIII AAGCTT 1 cut(s) 125
HpaII CCGG 1 cut(s) 399
HphI GGTGA 1 cut(s) 371
Hpy166II GTNNAC 1 cut(s) 168
Hpy188I TCNGA 2 cut(s) 84, 276
Hpy188III TCNNGA 2 cut(s) 323, 351
Hpy8I GTNNAC 1 cut(s) 168
HpyCH4III ACNGT 1 cut(s) 36
HpyCH4V TGCA 5 cut(s) 8, 176, 215, 280, 303
HpyF10VI GCNNNNNNNGC 1 cut(s) 14
Hsp92II CATG 4 cut(s) 12, 102, 158, 326
Kzo9I GATC 3 cut(s) 67, 181, 413
LmnI GCTCC 1 cut(s) 110
LpnPI CCDG 9 cut(s) 61, 69, 99, 180, 192, 261, 282, 283, 412
Lsp1109I GCAGC 3 cut(s) 31, 199, 227
LweI GCATC 1 cut(s) 267
MalI GATC 3 cut(s) 69, 183, 415
MboI GATC 3 cut(s) 67, 181, 413
MflI RGATCY 1 cut(s) 413
MluCI AATT 2 cut(s) 240, 364
MmeI TCCRAC 1 cut(s) 443
MnlI CCTC 4 cut(s) 52, 100, 365, 373
MseI TTAA 2 cut(s) 384, 454
MslI CAYNNNNRTG 2 cut(s) 153, 203
MspA1I CMGCKG 1 cut(s) 296
MspI CCGG 1 cut(s) 399
MspR9I CCNGG 1 cut(s) 400
MwoI GCNNNNNNNGC 1 cut(s) 14
NciI CCSGG 1 cut(s) 400
NcoI CCATGG 1 cut(s) 154
NdeII GATC 3 cut(s) 67, 181, 413
NlaIII CATG 4 cut(s) 12, 102, 158, 326
NspI RCATGY 2 cut(s) 12, 102
PagI TCATGA 1 cut(s) 322
PkrI GCNGC 5 cut(s) 7, 46, 214, 217, 295
PstI CTGCAG 1 cut(s) 217
PsuI RGATCY 1 cut(s) 413
RsaI GTAC 1 cut(s) 169
RsaNI GTAC 1 cut(s) 168
RseI CAYNNNNRTG 2 cut(s) 153, 203
SaqAI TTAA 2 cut(s) 384, 454
SatI GCNGC 5 cut(s) 6, 45, 213, 216, 294
Sau3AI GATC 3 cut(s) 67, 181, 413
ScrFI CCNGG 1 cut(s) 400
SetI ASST 7 cut(s) 63, 129, 181, 220, 332, 376, 384
SfaNI GCATC 1 cut(s) 267
SfcI CTRYAG 2 cut(s) 213, 409
SmiMI CAYNNNNRTG 2 cut(s) 153, 203
Sse9I AATT 2 cut(s) 240, 364
SsiI CCGC 2 cut(s) 294, 423
StyD4I CCNGG 1 cut(s) 398
StyI CCWWGG 2 cut(s) 154, 330
TaaI ACNGT 1 cut(s) 36
TaqI TCGA 2 cut(s) 76, 352
TasI AATT 2 cut(s) 240, 364
TatI WGTACW 1 cut(s) 167
TauI GCSGC 1 cut(s) 296
Tru1I TTAA 2 cut(s) 384, 454
Tru9I TTAA 2 cut(s) 384, 454
TscAI CASTG 2 cut(s) 351, 442
TseI GCWGC 4 cut(s) 5, 44, 212, 215
TspDTI ATGAA 1 cut(s) 223
TspRI CASTG 2 cut(s) 351, 442
XapI RAATTY 1 cut(s) 364
XceI RCATGY 2 cut(s) 12, 102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.