Rh5BG213100

EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0001544
Physical Location & Seq
Forward (+)
24665287 .. 24666751
1465 bp
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UTR
Exon/CDS
Intron
Rh5BG213100.1

Sequence Viewer

Length: 354 bp
ATGGCCCTGCTAACCACGTGCAATGGTTTTAGACCTGTAGAAGAGTCTGTATTTGAAAAGCGCATGGCAAATGTAAACGAACTGCGGAAGCTTGGAGCAAAAATTCAAGTCTGTGCAAGCTCTGCTCTGGTTTTCGGGAAAGATAATAGAAGTGGTTTGTCTGGTTCCTGCCTTGCTGCAACTGACCTCAGAGGTGGGATATCATTGGTATTAGGTGCATTGGCTGCAGAAGCTATTGACAAAGCTGTATTTTTGGGAGATAGAGGAGGAATTTGGTTGGGCTTTAGAGTGAAATTGCTCTCCTACAAATTCTTCTGGCTGACTTCAACCTCCCATAGAAGACTCTTCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPSP_synthase

Protein Analysis

117

Amino Acids

12.73

Weight (kDa)

9.85

Isoelectric Point (pI)

33.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPSP_synthase PF00275 2 - 78 3.7e-07 EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 85
AcsI RAATTY 3 cut(s) 102, 270, 308
AcvI CACGTG 1 cut(s) 18
AgsI TTSAA 3 cut(s) 56, 107, 327
AluBI AGCT 4 cut(s) 91, 120, 233, 245
AluI AGCT 4 cut(s) 91, 120, 233, 245
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 2 cut(s) 176, 224
ApoI RAATTY 3 cut(s) 102, 270, 308
ArsI GACNNNNNNTTYG 1 cut(s) 333
AspLEI GCGC 1 cut(s) 63
AspS9I GGNCC 1 cut(s) 4
BbrPI CACGTG 1 cut(s) 18
BbsI GAAGAC 1 cut(s) 346
BbvI GCAGC 2 cut(s) 163, 211
BfmI CTRYAG 2 cut(s) 36, 225
BisI GCNGC 2 cut(s) 177, 225
BlsI GCNGC 2 cut(s) 178, 226
BmgT120I GGNCC 1 cut(s) 4
BmiI GGNNCC 1 cut(s) 166
BpiI GAAGAC 1 cut(s) 346
BsaAI YACGTR 1 cut(s) 18
Bse3DI GCAATG 1 cut(s) 28
BseMI GCAATG 1 cut(s) 28
BseMII CTCAG 1 cut(s) 202
BseRI GAGGAG 1 cut(s) 279
BseXI GCAGC 2 cut(s) 163, 211
BshFI GGCC 1 cut(s) 5
BsnI GGCC 1 cut(s) 5
BspACI CCGC 1 cut(s) 85
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 1 cut(s) 201
BspLI GGNNCC 1 cut(s) 166
BspMAI CTGCAG 1 cut(s) 229
BsrDI GCAATG 1 cut(s) 28
Bst6I CTCTTC 2 cut(s) 36, 350
BstAPI GCANNNNNTGC 2 cut(s) 122, 224
BstBAI YACGTR 1 cut(s) 18
BstC8I GCNNGC 1 cut(s) 118
BstDEI CTNAG 1 cut(s) 188
BstHHI GCGC 1 cut(s) 63
BstMWI GCNNNNNNNGC 3 cut(s) 122, 224, 230
BstSFI CTRYAG 2 cut(s) 36, 225
BstV1I GCAGC 2 cut(s) 163, 211
BstV2I GAAGAC 1 cut(s) 346
BsuRI GGCC 1 cut(s) 5
Cac8I GCNNGC 1 cut(s) 118
CfoI GCGC 1 cut(s) 63
Cfr13I GGNCC 1 cut(s) 4
CviAII CATG 1 cut(s) 64
CviJI RGCY 8 cut(s) 5, 91, 120, 224, 233, 245, 282, 319
CviKI_1 RGCY 8 cut(s) 5, 91, 120, 224, 233, 245, 282, 319
DdeI CTNAG 1 cut(s) 188
Eam1104I CTCTTC 2 cut(s) 36, 350
EarI CTCTTC 2 cut(s) 36, 350
Eco32I GATATC 1 cut(s) 201
Eco72I CACGTG 1 cut(s) 18
EcoRV GATATC 1 cut(s) 201
FaeI CATG 1 cut(s) 67
FaiI YATR 2 cut(s) 65, 336
FatI CATG 1 cut(s) 63
Fnu4HI GCNGC 2 cut(s) 177, 225
Fsp4HI GCNGC 2 cut(s) 177, 225
GlaI GCGC 1 cut(s) 62
GluI GCNGC 2 cut(s) 177, 225
HaeIII GGCC 1 cut(s) 5
HhaI GCGC 1 cut(s) 63
Hin1II CATG 1 cut(s) 67
Hin6I GCGC 1 cut(s) 61
HinP1I GCGC 1 cut(s) 61
HindIII AAGCTT 1 cut(s) 89
HinfI GANTC 2 cut(s) 44, 342
Hpy166II GTNNAC 1 cut(s) 76
Hpy188I TCNGA 1 cut(s) 191
Hpy188III TCNNGA 1 cut(s) 136
Hpy8I GTNNAC 1 cut(s) 76
HpyCH4IV ACGT 1 cut(s) 17
HpyCH4V TGCA 5 cut(s) 21, 116, 179, 218, 227
HpyF10VI GCNNNNNNNGC 3 cut(s) 122, 224, 230
HpyF3I CTNAG 1 cut(s) 188
HpySE526I ACGT 1 cut(s) 17
Hsp92II CATG 1 cut(s) 67
HspAI GCGC 1 cut(s) 61
LmnI GCTCC 1 cut(s) 95
LpnPI CCDG 6 cut(s) 20, 48, 113, 147, 181, 301
Lsp1109I GCAGC 2 cut(s) 163, 211
MaeII ACGT 1 cut(s) 17
MboII GAAGA 4 cut(s) 53, 304, 337, 351
MluCI AATT 4 cut(s) 102, 270, 293, 308
MlyI GAGTC 2 cut(s) 53, 336
MnlI CCTC 5 cut(s) 185, 197, 257, 260, 340
MwoI GCNNNNNNNGC 3 cut(s) 122, 224, 230
NlaIII CATG 1 cut(s) 67
NlaIV GGNNCC 1 cut(s) 166
PkrI GCNGC 2 cut(s) 178, 226
PleI GAGTC 2 cut(s) 52, 336
PmaCI CACGTG 1 cut(s) 18
PmlI CACGTG 1 cut(s) 18
PpsI GAGTC 2 cut(s) 52, 336
Ppu21I YACGTR 1 cut(s) 18
PspCI CACGTG 1 cut(s) 18
PspN4I GGNNCC 1 cut(s) 166
PspPI GGNCC 1 cut(s) 4
PstI CTGCAG 1 cut(s) 229
SatI GCNGC 2 cut(s) 177, 225
Sau96I GGNCC 1 cut(s) 4
SchI GAGTC 2 cut(s) 53, 336
SfcI CTRYAG 2 cut(s) 36, 225
Sse9I AATT 4 cut(s) 102, 270, 293, 308
SsiI CCGC 1 cut(s) 85
TaiI ACGT 1 cut(s) 20
TasI AATT 4 cut(s) 102, 270, 293, 308
TseI GCWGC 2 cut(s) 176, 224
TspDTI ATGAA 1 cut(s) 337
XapI RAATTY 3 cut(s) 102, 270, 308
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.