RLG00000033292

EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0001544
Physical Location & Seq
Reverse (-)
25359561 .. 25363234
3674 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033292

Sequence Viewer

Length: 1014 bp
ATGTACCCATCAGTGGCTCCAAGAACTCAGCCTTGCCCATTCTCGCAGCTACCCTTTGCTGATCAGGCACTTCAGTCATTCACATGTGCCCAATTTGTCAGATACTCGCACAATGGCTTCAGTCTTGGCCTCTCTGGACCGGTGGACCTTTACATTCGGGGTCTTCAAGCTCTTGGTGCTGTTGTGGAGATGAAGGATGGCAAAGTTCAGGCATATGCATTAAATGGAAGAGGATTGGTTGGCGGGAGTTTCCAACTTGATTACCCTAGCGTTGGGGCAACAGAAACCCTTCTGATGGCTGCATGTATGGCTGATGGAACAACTATACTTGGGGCCTGTGTGGAAGGAGCAGGAAGCAACAAGCTTGTCATCAAGGGAAAATCTCATTTGCATGGTTGTAAATGTACTATTGCACCTGATCGTATTGAAGCAGGCACGTTTATGCTTGCTGCAGCTATTACTCGCTCATTCATTTCAATTTCACCTGTCATTCCTTCCCAAGTTTCCTGTCTGATGCAAAAACTCTTGGCCGCTGGTTGCAAAATAAGGCAATGCTCTCATGATACCTTGGAAATATCAGTGTCTCAATGTGGTGAGAATTTGCGAGATTTTGAGGTTAAGACAGGGCCATTTCCCAGGTTTCCTACAGATCTCCAACCGCAAACAATGGCCCTGCTAACCACGTGCAATGGTTTACGTCCTGTAGAAGAGTCTGTATTTGAAAAGCCCATGGCTCATGTAAACGAACTGCGGAAGCTTGGAGCAAAAATTCAAGTCTGCGCAAGCTCTGCTCTGGTTTTTGGGAAAGATAATAGAAGTGGTTTCTCTGGTTCCTGCCTTGCTGCAACTGACCTCGGAGGTGGGATATCATTGGTATTAGCTGCATTGGCTGCAGAAGGTACTACTGAGATCAGCGGTGTTGCTCATATTGACCGTGGTTATGAGAATGTAGATACAAAAATTCAAATGCTGGGAGCCGATATCAAAAGATTAACGGCCCCTGCTTCTTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPSP_synthase

Protein Analysis

338

Amino Acids

35.51

Weight (kDa)

7.48

Isoelectric Point (pI)

40.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPSP_synthase PF00275 47 - 317 2.6e-42 EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 781
AciI CCGC 5 cut(s) 243, 531, 659, 751, 915
AcoI YGGCCR 1 cut(s) 528
AcsI RAATTY 3 cut(s) 598, 768, 960
AcuI CTGAAG 2 cut(s) 56, 103
AcvI CACGTG 1 cut(s) 684
AfaI GTAC 3 cut(s) 5, 406, 901
AfiI CCNNNNNNNGG 3 cut(s) 13, 272, 295
AflIII ACRYGT 1 cut(s) 83
AgeI ACCGGT 1 cut(s) 139
AgsI TTSAA 6 cut(s) 167, 428, 477, 722, 773, 965
AjnI CCWGG 1 cut(s) 635
AluBI AGCT 7 cut(s) 49, 170, 364, 455, 757, 786, 881
AluI AGCT 7 cut(s) 49, 170, 364, 455, 757, 786, 881
Alw26I GTCTC 1 cut(s) 588
AoxI GGCC 6 cut(s) 127, 333, 528, 626, 669, 996
ApeKI GCWGC 7 cut(s) 46, 299, 449, 452, 842, 881, 890
ApoI RAATTY 3 cut(s) 598, 768, 960
AsiGI ACCGGT 1 cut(s) 139
Asp700I GAANNNNTTC 1 cut(s) 288
AspLEI GCGC 1 cut(s) 782
AspS9I GGNCC 6 cut(s) 137, 145, 333, 626, 670, 997
AsuHPI GGTGA 2 cut(s) 474, 605
AvaII GGWCC 2 cut(s) 137, 145
BaeGI GKGCMC 1 cut(s) 91
BbrPI CACGTG 1 cut(s) 684
BbsI GAAGAC 1 cut(s) 155
BbvI GCAGC 7 cut(s) 58, 286, 436, 464, 829, 868, 877
BccI CCATC 4 cut(s) 16, 191, 289, 308
BceAI ACGGC 1 cut(s) 1011
BciT130I CCWGG 1 cut(s) 637
BclI TGATCA 1 cut(s) 61
BcoDI GTCTC 1 cut(s) 588
BfaI CTAG 2 cut(s) 267, 1012
BfmI CTRYAG 4 cut(s) 450, 645, 702, 891
BglII AGATCT 1 cut(s) 649
BisI GCNGC 8 cut(s) 47, 300, 450, 453, 531, 843, 882, 891
BlsI GCNGC 8 cut(s) 48, 301, 451, 454, 532, 844, 883, 892
Bme1390I CCNGG 1 cut(s) 637
Bme18I GGWCC 2 cut(s) 137, 145
BmgT120I GGNCC 6 cut(s) 137, 145, 333, 626, 670, 997
BmiI GGNNCC 5 cut(s) 18, 334, 832, 976, 999
BmrFI CCNGG 1 cut(s) 637
BmsI GCATC 1 cut(s) 504
BpiI GAAGAC 1 cut(s) 155
BsaAI YACGTR 1 cut(s) 684
BsaJI CCNNGG 5 cut(s) 567, 635, 729, 853, 934
BsaWI WCCGGW 1 cut(s) 139
Bsc4I CCNNNNNNNGG 3 cut(s) 13, 272, 295
Bse118I RCCGGY 1 cut(s) 139
Bse3DI GCAATG 2 cut(s) 557, 694
BseBI CCWGG 1 cut(s) 637
BseDI CCNNGG 5 cut(s) 567, 635, 729, 853, 934
BseGI GGATG 1 cut(s) 202
BseLI CCNNNNNNNGG 3 cut(s) 13, 272, 295
BseMI GCAATG 2 cut(s) 557, 694
BseMII CTCAG 2 cut(s) 41, 897
BseSI GKGCMC 1 cut(s) 91
BseXI GCAGC 7 cut(s) 58, 286, 436, 464, 829, 868, 877
BseYI CCCAGC 1 cut(s) 970
BshFI GGCC 6 cut(s) 129, 335, 530, 628, 671, 998
BshTI ACCGGT 1 cut(s) 139
BsiSI CCGG 1 cut(s) 140
BslI CCNNNNNNNGG 3 cut(s) 13, 272, 295
BsmAI GTCTC 1 cut(s) 588
BsnI GGCC 6 cut(s) 129, 335, 530, 628, 671, 998
Bsp1286I GDGCHC 1 cut(s) 91
Bsp143I GATC 4 cut(s) 61, 418, 649, 909
Bsp19I CCATGG 1 cut(s) 729
BspACI CCGC 5 cut(s) 243, 531, 659, 751, 915
BspANI GGCC 6 cut(s) 129, 335, 530, 628, 671, 998
BspCNI CTCAG 2 cut(s) 40, 898
BspHI TCATGA 1 cut(s) 559
BspLI GGNNCC 5 cut(s) 18, 334, 832, 976, 999
BspMAI CTGCAG 2 cut(s) 454, 895
BsrDI GCAATG 2 cut(s) 557, 694
BsrFI RCCGGY 1 cut(s) 139
BssAI RCCGGY 1 cut(s) 139
BssECI CCNNGG 5 cut(s) 567, 635, 729, 853, 934
BssMI GATC 4 cut(s) 61, 418, 649, 909
BssT1I CCWWGG 2 cut(s) 567, 729
Bst2UI CCWGG 1 cut(s) 637
Bst4CI ACNGT 1 cut(s) 935
Bst6I CTCTTC 2 cut(s) 223, 702
BstAPI GCANNNNNTGC 2 cut(s) 788, 890
BstBAI YACGTR 1 cut(s) 684
BstC8I GCNNGC 3 cut(s) 433, 447, 784
BstDEI CTNAG 2 cut(s) 27, 906
BstDSI CCRYGG 2 cut(s) 729, 934
BstF5I GGATG 1 cut(s) 202
BstHHI GCGC 1 cut(s) 782
BstKTI GATC 4 cut(s) 64, 421, 652, 912
BstMAI GTCTC 1 cut(s) 588
BstMBI GATC 4 cut(s) 61, 418, 649, 909
BstMWI GCNNNNNNNGC 6 cut(s) 65, 176, 308, 788, 887, 890
BstNI CCWGG 1 cut(s) 637
BstNSI RCATGY 2 cut(s) 87, 306
BstSCI CCNGG 1 cut(s) 635
BstSFI CTRYAG 4 cut(s) 450, 645, 702, 891
BstSLI GKGCMC 1 cut(s) 91
BstV1I GCAGC 7 cut(s) 58, 286, 436, 464, 829, 868, 877
BstV2I GAAGAC 1 cut(s) 155
BstX2I RGATCY 1 cut(s) 649
BstYI RGATCY 1 cut(s) 649
BsuRI GGCC 6 cut(s) 129, 335, 530, 628, 671, 998
BtgI CCRYGG 2 cut(s) 729, 934
BtsCI GGATG 1 cut(s) 202
BtsIMutI CAGTG 2 cut(s) 18, 585
Cac8I GCNNGC 3 cut(s) 433, 447, 784
CciI TCATGA 1 cut(s) 559
CfoI GCGC 1 cut(s) 782
Cfr10I RCCGGY 1 cut(s) 139
Cfr13I GGNCC 6 cut(s) 137, 145, 333, 626, 670, 997
Csp6I GTAC 3 cut(s) 4, 405, 900
CspAI ACCGGT 1 cut(s) 139
CviAII CATG 6 cut(s) 84, 303, 392, 560, 730, 737
CviQI GTAC 3 cut(s) 4, 405, 900
DdeI CTNAG 2 cut(s) 27, 906
DpnI GATC 4 cut(s) 63, 420, 651, 911
DpnII GATC 4 cut(s) 61, 418, 649, 909
EaeI YGGCCR 1 cut(s) 528
Eam1104I CTCTTC 2 cut(s) 223, 702
EarI CTCTTC 2 cut(s) 223, 702
Eco130I CCWWGG 2 cut(s) 567, 729
Eco32I GATATC 2 cut(s) 867, 982
Eco47I GGWCC 2 cut(s) 137, 145
Eco57I CTGAAG 2 cut(s) 56, 103
Eco72I CACGTG 1 cut(s) 684
EcoO109I RGGNCCY 1 cut(s) 333
EcoRII CCWGG 1 cut(s) 635
EcoRV GATATC 2 cut(s) 867, 982
EcoT14I CCWWGG 2 cut(s) 567, 729
EcoT22I ATGCAT 1 cut(s) 220
ErhI CCWWGG 2 cut(s) 567, 729
FaeI CATG 6 cut(s) 87, 306, 395, 563, 733, 740
FatI CATG 6 cut(s) 83, 302, 391, 559, 729, 736
FauI CCCGC 1 cut(s) 236
FauNDI CATATG 1 cut(s) 214
FbaI TGATCA 1 cut(s) 61
Fnu4HI GCNGC 8 cut(s) 47, 300, 450, 453, 531, 843, 882, 891
FokI GGATG 1 cut(s) 209
Fsp4HI GCNGC 8 cut(s) 47, 300, 450, 453, 531, 843, 882, 891
FspBI CTAG 2 cut(s) 267, 1012
FspI TGCGCA 1 cut(s) 781
GlaI GCGC 1 cut(s) 781
GluI GCNGC 8 cut(s) 47, 300, 450, 453, 531, 843, 882, 891
GsaI CCCAGC 1 cut(s) 974
HaeIII GGCC 6 cut(s) 129, 335, 530, 628, 671, 998
HapII CCGG 1 cut(s) 140
HhaI GCGC 1 cut(s) 782
Hin1II CATG 6 cut(s) 87, 306, 395, 563, 733, 740
Hin6I GCGC 1 cut(s) 780
HinP1I GCGC 1 cut(s) 780
HindIII AAGCTT 2 cut(s) 362, 755
HinfI GANTC 1 cut(s) 710
HpaII CCGG 1 cut(s) 140
HphI GGTGA 2 cut(s) 474, 605
Hpy166II GTNNAC 3 cut(s) 145, 695, 742
Hpy188I TCNGA 4 cut(s) 101, 294, 513, 857
Hpy188III TCNNGA 2 cut(s) 135, 560
Hpy8I GTNNAC 3 cut(s) 145, 695, 742
HpyAV CCTTC 5 cut(s) 187, 299, 338, 504, 890
HpyCH4III ACNGT 1 cut(s) 935
HpyCH4IV ACGT 3 cut(s) 437, 683, 697
HpyF10VI GCNNNNNNNGC 6 cut(s) 65, 176, 308, 788, 887, 890
HpyF3I CTNAG 2 cut(s) 27, 906
HpySE526I ACGT 3 cut(s) 437, 683, 697
Hsp92II CATG 6 cut(s) 87, 306, 395, 563, 733, 740
HspAI GCGC 1 cut(s) 780
Ksp22I TGATCA 1 cut(s) 61
Kzo9I GATC 4 cut(s) 61, 418, 649, 909
LmnI GCTCC 4 cut(s) 22, 347, 761, 974
Lsp1109I GCAGC 7 cut(s) 58, 286, 436, 464, 829, 868, 877
LweI GCATC 1 cut(s) 504
MaeI CTAG 2 cut(s) 267, 1012
MaeII ACGT 3 cut(s) 437, 683, 697
MalI GATC 4 cut(s) 63, 420, 651, 911
MboI GATC 4 cut(s) 61, 418, 649, 909
MboII GAAGA 4 cut(s) 155, 240, 719, 999
MflI RGATCY 1 cut(s) 649
MhlI GDGCHC 1 cut(s) 91
MluCI AATT 5 cut(s) 92, 477, 598, 768, 960
MlyI GAGTC 1 cut(s) 719
MmeI TCCRAC 2 cut(s) 277, 679
MnlI CCTC 5 cut(s) 140, 224, 607, 851, 863
Mph1103I ATGCAT 1 cut(s) 220
MroXI GAANNNNTTC 1 cut(s) 288
MseI TTAA 3 cut(s) 221, 618, 992
MslI CAYNNNNRTG 3 cut(s) 82, 390, 440
MspA1I CMGCKG 2 cut(s) 533, 915
MspI CCGG 1 cut(s) 140
MspR9I CCNGG 1 cut(s) 637
MvaI CCWGG 1 cut(s) 637
MwoI GCNNNNNNNGC 6 cut(s) 65, 176, 308, 788, 887, 890
NcoI CCATGG 1 cut(s) 729
NdeI CATATG 1 cut(s) 214
NdeII GATC 4 cut(s) 61, 418, 649, 909
NlaIII CATG 6 cut(s) 87, 306, 395, 563, 733, 740
NlaIV GGNNCC 5 cut(s) 18, 334, 832, 976, 999
NsbI TGCGCA 1 cut(s) 781
NsiI ATGCAT 1 cut(s) 220
NspI RCATGY 2 cut(s) 87, 306
PagI TCATGA 1 cut(s) 559
PciI ACATGT 1 cut(s) 83
PdmI GAANNNNTTC 1 cut(s) 288
PinAI ACCGGT 1 cut(s) 139
PkrI GCNGC 8 cut(s) 48, 301, 451, 454, 532, 844, 883, 892
PleI GAGTC 1 cut(s) 718
PmaCI CACGTG 1 cut(s) 684
PmlI CACGTG 1 cut(s) 684
PpsI GAGTC 1 cut(s) 718
Ppu21I YACGTR 1 cut(s) 684
PscI ACATGT 1 cut(s) 83
Psp6I CCWGG 1 cut(s) 635
PspCI CACGTG 1 cut(s) 684
PspFI CCCAGC 1 cut(s) 970
PspGI CCWGG 1 cut(s) 635
PspN4I GGNNCC 5 cut(s) 18, 334, 832, 976, 999
PspPI GGNCC 6 cut(s) 137, 145, 333, 626, 670, 997
PstI CTGCAG 2 cut(s) 454, 895
PsuI RGATCY 1 cut(s) 649
RsaI GTAC 3 cut(s) 5, 406, 901
RsaNI GTAC 3 cut(s) 4, 405, 900
RseI CAYNNNNRTG 3 cut(s) 82, 390, 440
SaqAI TTAA 3 cut(s) 221, 618, 992
SatI GCNGC 8 cut(s) 47, 300, 450, 453, 531, 843, 882, 891
Sau3AI GATC 4 cut(s) 61, 418, 649, 909
Sau96I GGNCC 6 cut(s) 137, 145, 333, 626, 670, 997
SchI GAGTC 1 cut(s) 719
ScrFI CCNGG 1 cut(s) 637
SduI GDGCHC 1 cut(s) 91
SfaNI GCATC 1 cut(s) 504
SfcI CTRYAG 4 cut(s) 450, 645, 702, 891
SinI GGWCC 2 cut(s) 137, 145
SmiMI CAYNNNNRTG 3 cut(s) 82, 390, 440
Sse9I AATT 5 cut(s) 92, 477, 598, 768, 960
SsiI CCGC 5 cut(s) 243, 531, 659, 751, 915
SspMI CTAG 2 cut(s) 267, 1012
StyD4I CCNGG 1 cut(s) 635
StyI CCWWGG 2 cut(s) 567, 729
TaaI ACNGT 1 cut(s) 935
TaiI ACGT 3 cut(s) 440, 686, 700
TasI AATT 5 cut(s) 92, 477, 598, 768, 960
TatI WGTACW 1 cut(s) 404
TauI GCSGC 1 cut(s) 533
Tru1I TTAA 3 cut(s) 221, 618, 992
Tru9I TTAA 3 cut(s) 221, 618, 992
TscAI CASTG 2 cut(s) 18, 585
TseI GCWGC 7 cut(s) 46, 299, 449, 452, 842, 881, 890
TspDTI ATGAA 2 cut(s) 206, 460
TspRI CASTG 2 cut(s) 18, 585
VpaK11BI GGWCC 2 cut(s) 137, 145
XapI RAATTY 3 cut(s) 598, 768, 960
XceI RCATGY 2 cut(s) 87, 306
XmnI GAANNNNTTC 1 cut(s) 288
XspI CTAG 2 cut(s) 267, 1012
Zsp2I ATGCAT 1 cut(s) 220
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.