Rw0G012990

EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00577
OG0001544
Physical Location & Seq
Forward (+)
27431 .. 29368
1938 bp
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UTR
Exon/CDS
Intron
Rw0G012990.1

Sequence Viewer

Length: 363 bp
ATGGTTCAGGCATATGCATTAAATGGAAGAGGATTAGTTGGGGGAAGTTTCCAACTTGATTACCCTAGTGTTGGTGCAACAGAAACCCTTCTAATGGCTGCATGTATGGCTGATGGAACAACTATACTGTCCAATGTTGCCAGAGAACCAGAGGTGGTTGATCTTGCTCGATTTCTGACTAAAAGTGGGGCCTGTGTGGAAGGAGCAGGAAGCTTGACATCAAGGGAAAATCTCATTTGCATGGTTGTAAATGTACTATTGCACCTGATCGTATTGAAGCAGGCACATTTATGCTTGCTGCAGCTATTACTCGCTCATTCATTTCAATTTCACCTGTCATTCCTTACCAAGTTTCCTGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPSP_synthase

Protein Analysis

120

Amino Acids

12.89

Weight (kDa)

6.38

Isoelectric Point (pI)

35.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPSP_synthase PF00275 10 - 71 2.6e-12 EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfaI GTAC 1 cut(s) 255
AfiI CCNNNNNNNGG 2 cut(s) 71, 94
AgsI TTSAA 2 cut(s) 277, 326
AluBI AGCT 2 cut(s) 213, 304
AluI AGCT 2 cut(s) 213, 304
AoxI GGCC 1 cut(s) 189
ApeKI GCWGC 3 cut(s) 98, 298, 301
Asp700I GAANNNNTTC 1 cut(s) 87
AspS9I GGNCC 1 cut(s) 189
AsuHPI GGTGA 1 cut(s) 323
BbvI GCAGC 3 cut(s) 85, 285, 313
BccI CCATC 1 cut(s) 107
BfaI CTAG 1 cut(s) 66
BfmI CTRYAG 1 cut(s) 299
BisI GCNGC 3 cut(s) 99, 299, 302
BlsI GCNGC 3 cut(s) 100, 300, 303
BmgT120I GGNCC 1 cut(s) 189
BmiI GGNNCC 1 cut(s) 190
Bsc4I CCNNNNNNNGG 2 cut(s) 71, 94
BseLI CCNNNNNNNGG 2 cut(s) 71, 94
BseXI GCAGC 3 cut(s) 85, 285, 313
BshFI GGCC 1 cut(s) 191
BslI CCNNNNNNNGG 2 cut(s) 71, 94
BsnI GGCC 1 cut(s) 191
Bsp143I GATC 2 cut(s) 160, 267
BspANI GGCC 1 cut(s) 191
BspLI GGNNCC 1 cut(s) 190
BspMAI CTGCAG 1 cut(s) 303
BssMI GATC 2 cut(s) 160, 267
Bst4CI ACNGT 1 cut(s) 129
Bst6I CTCTTC 1 cut(s) 22
BstC8I GCNNGC 2 cut(s) 282, 296
BstKTI GATC 2 cut(s) 163, 270
BstMBI GATC 2 cut(s) 160, 267
BstMWI GCNNNNNNNGC 1 cut(s) 107
BstNSI RCATGY 1 cut(s) 105
BstSFI CTRYAG 1 cut(s) 299
BstV1I GCAGC 3 cut(s) 85, 285, 313
BsuRI GGCC 1 cut(s) 191
Cac8I GCNNGC 2 cut(s) 282, 296
Cfr13I GGNCC 1 cut(s) 189
Csp6I GTAC 1 cut(s) 254
CviAII CATG 2 cut(s) 102, 241
CviJI RGCY 5 cut(s) 98, 110, 191, 213, 304
CviKI_1 RGCY 5 cut(s) 98, 110, 191, 213, 304
CviQI GTAC 1 cut(s) 254
DpnI GATC 2 cut(s) 162, 269
DpnII GATC 2 cut(s) 160, 267
Eam1104I CTCTTC 1 cut(s) 22
EarI CTCTTC 1 cut(s) 22
EcoO109I RGGNCCY 1 cut(s) 189
EcoT22I ATGCAT 1 cut(s) 19
FaeI CATG 2 cut(s) 105, 244
FaiI YATR 7 cut(s) 13, 15, 103, 107, 125, 242, 292
FatI CATG 2 cut(s) 101, 240
FauNDI CATATG 1 cut(s) 13
Fnu4HI GCNGC 3 cut(s) 99, 299, 302
Fsp4HI GCNGC 3 cut(s) 99, 299, 302
FspBI CTAG 1 cut(s) 66
GluI GCNGC 3 cut(s) 99, 299, 302
HaeIII GGCC 1 cut(s) 191
Hin1II CATG 2 cut(s) 105, 244
HindIII AAGCTT 1 cut(s) 211
HphI GGTGA 1 cut(s) 323
Hpy188I TCNGA 2 cut(s) 177, 362
HpyAV CCTTC 2 cut(s) 98, 194
HpyCH4III ACNGT 1 cut(s) 129
HpyCH4V TGCA 6 cut(s) 17, 77, 101, 240, 262, 301
HpyF10VI GCNNNNNNNGC 1 cut(s) 107
Hsp92II CATG 2 cut(s) 105, 244
Kzo9I GATC 2 cut(s) 160, 267
LmnI GCTCC 1 cut(s) 203
LpnPI CCDG 7 cut(s) 154, 162, 192, 205, 266, 278, 347
Lsp1109I GCAGC 3 cut(s) 85, 285, 313
MaeI CTAG 1 cut(s) 66
MalI GATC 2 cut(s) 162, 269
MboI GATC 2 cut(s) 160, 267
MboII GAAGA 1 cut(s) 39
MluCI AATT 1 cut(s) 326
MmeI TCCRAC 1 cut(s) 76
MnlI CCTC 2 cut(s) 23, 145
Mph1103I ATGCAT 1 cut(s) 19
MroXI GAANNNNTTC 1 cut(s) 87
MseI TTAA 1 cut(s) 20
MslI CAYNNNNRTG 2 cut(s) 239, 289
MwoI GCNNNNNNNGC 1 cut(s) 107
NdeI CATATG 1 cut(s) 13
NdeII GATC 2 cut(s) 160, 267
NlaIII CATG 2 cut(s) 105, 244
NlaIV GGNNCC 1 cut(s) 190
NsiI ATGCAT 1 cut(s) 19
NspI RCATGY 1 cut(s) 105
PdmI GAANNNNTTC 1 cut(s) 87
PkrI GCNGC 3 cut(s) 100, 300, 303
PspN4I GGNNCC 1 cut(s) 190
PspPI GGNCC 1 cut(s) 189
PstI CTGCAG 1 cut(s) 303
RsaI GTAC 1 cut(s) 255
RsaNI GTAC 1 cut(s) 254
RseI CAYNNNNRTG 2 cut(s) 239, 289
SaqAI TTAA 1 cut(s) 20
SatI GCNGC 3 cut(s) 99, 299, 302
Sau3AI GATC 2 cut(s) 160, 267
Sau96I GGNCC 1 cut(s) 189
SetI ASST 5 cut(s) 156, 215, 267, 306, 336
SfcI CTRYAG 1 cut(s) 299
SmiMI CAYNNNNRTG 2 cut(s) 239, 289
Sse9I AATT 1 cut(s) 326
SspMI CTAG 1 cut(s) 66
TaaI ACNGT 1 cut(s) 129
TaqI TCGA 1 cut(s) 169
TasI AATT 1 cut(s) 326
TatI WGTACW 1 cut(s) 253
Tru1I TTAA 1 cut(s) 20
Tru9I TTAA 1 cut(s) 20
TseI GCWGC 3 cut(s) 98, 298, 301
TspDTI ATGAA 1 cut(s) 309
XceI RCATGY 1 cut(s) 105
XmnI GAANNNNTTC 1 cut(s) 87
XspI CTAG 1 cut(s) 66
Zsp2I ATGCAT 1 cut(s) 19
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.