Rh5BG213000

EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0001544
Physical Location & Seq
Forward (+)
24662667 .. 24664913
2247 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG213000.1

Sequence Viewer

Length: 432 bp
ATGTTGAGCCTAATTCGGAAGATCCGAGGTGGGTTTTTCGTTCTTGGGCCGCCGGTAGCCCGGTTCGGTGAGGCTGTGGTTGCATTGCCCGGTGGATGTGACATTGGGGCTAGACCGGTGGACCTTTACATTCGGGGTCTTCAAGCTCTTGGTGCTGTTGTGGAGATGAAGGATGGCAAAGTTCAGGCATATGCATTAAATGGAAGAGGATTGGTTGGGGGAAGTTTCCAACTTGATTACCCTAGCGTTAGGGCAACAGAAACCCTTCTGATGGCTGCATGTATGGCTGATGGAACAACTATACTGTCCATTGTTGCCAGAGAACCAGTGGTTGATCTTGCTCGATTTCTGACTAAAAGTGGGGCCTGTGTGGAAGGAGCAGAAAGCAACAAAGCTTGTCATCAAGGGAAAATCTCATTTTCATGGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPSP_synthase

Protein Analysis

143

Amino Acids

15.04

Weight (kDa)

8.78

Isoelectric Point (pI)

37.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPSP_synthase PF00275 5 - 131 1.6e-22 EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 50
AclWI GGATC 1 cut(s) 16
AfiI CCNNNNNNNGG 1 cut(s) 271
AgeI ACCGGT 1 cut(s) 115
AgsI TTSAA 1 cut(s) 143
AluBI AGCT 2 cut(s) 146, 395
AluI AGCT 2 cut(s) 146, 395
AlwI GGATC 1 cut(s) 16
AoxI GGCC 2 cut(s) 47, 363
ApeKI GCWGC 1 cut(s) 275
AsiGI ACCGGT 1 cut(s) 115
Asp700I GAANNNNTTC 1 cut(s) 264
AspS9I GGNCC 3 cut(s) 47, 121, 363
AsuC2I CCSGG 2 cut(s) 61, 90
AsuHPI GGTGA 1 cut(s) 80
AvaII GGWCC 1 cut(s) 121
BbsI GAAGAC 1 cut(s) 131
BbvI GCAGC 1 cut(s) 262
BccI CCATC 3 cut(s) 167, 265, 284
BcnI CCSGG 2 cut(s) 61, 90
BfaI CTAG 2 cut(s) 111, 243
BisI GCNGC 2 cut(s) 50, 276
BlsI GCNGC 2 cut(s) 51, 277
Bme1390I CCNGG 2 cut(s) 61, 90
Bme18I GGWCC 1 cut(s) 121
BmgT120I GGNCC 3 cut(s) 47, 121, 363
BmiI GGNNCC 1 cut(s) 364
BmrFI CCNGG 2 cut(s) 61, 90
BpiI GAAGAC 1 cut(s) 131
BpuMI CCSGG 2 cut(s) 61, 90
BsaJI CCNNGG 1 cut(s) 25
BsaWI WCCGGW 1 cut(s) 115
Bsc4I CCNNNNNNNGG 1 cut(s) 271
Bse118I RCCGGY 2 cut(s) 52, 115
Bse1I ACTGG 1 cut(s) 326
Bse3DI GCAATG 1 cut(s) 83
BseDI CCNNGG 1 cut(s) 25
BseGI GGATG 2 cut(s) 101, 178
BseLI CCNNNNNNNGG 1 cut(s) 271
BseMI GCAATG 1 cut(s) 83
BseNI ACTGG 1 cut(s) 326
BseXI GCAGC 1 cut(s) 262
BshFI GGCC 2 cut(s) 49, 365
BshTI ACCGGT 1 cut(s) 115
BsiSI CCGG 4 cut(s) 53, 61, 90, 116
BslI CCNNNNNNNGG 1 cut(s) 271
BsnI GGCC 2 cut(s) 49, 365
Bsp143I GATC 2 cut(s) 21, 334
BspACI CCGC 1 cut(s) 50
BspANI GGCC 2 cut(s) 49, 365
BspLI GGNNCC 1 cut(s) 364
BspPI GGATC 1 cut(s) 16
BsrDI GCAATG 1 cut(s) 83
BsrFI RCCGGY 2 cut(s) 52, 115
BsrI ACTGG 1 cut(s) 326
BssAI RCCGGY 2 cut(s) 52, 115
BssECI CCNNGG 1 cut(s) 25
BssMI GATC 2 cut(s) 21, 334
Bst4CI ACNGT 1 cut(s) 306
Bst6I CTCTTC 1 cut(s) 199
BstF5I GGATG 2 cut(s) 101, 178
BstKTI GATC 2 cut(s) 24, 337
BstMBI GATC 2 cut(s) 21, 334
BstMWI GCNNNNNNNGC 3 cut(s) 80, 152, 284
BstNSI RCATGY 1 cut(s) 282
BstSCI CCNGG 2 cut(s) 59, 88
BstV1I GCAGC 1 cut(s) 262
BstV2I GAAGAC 1 cut(s) 131
BstX2I RGATCY 1 cut(s) 21
BstYI RGATCY 1 cut(s) 21
BsuRI GGCC 2 cut(s) 49, 365
BtsCI GGATG 2 cut(s) 101, 178
BtsIMutI CAGTG 1 cut(s) 333
Cfr10I RCCGGY 2 cut(s) 52, 115
Cfr13I GGNCC 3 cut(s) 47, 121, 363
CspAI ACCGGT 1 cut(s) 115
CviAII CATG 2 cut(s) 279, 423
DpnI GATC 2 cut(s) 23, 336
DpnII GATC 2 cut(s) 21, 334
Eam1104I CTCTTC 1 cut(s) 199
EarI CTCTTC 1 cut(s) 199
Eco47I GGWCC 1 cut(s) 121
EcoO109I RGGNCCY 1 cut(s) 363
EcoT22I ATGCAT 1 cut(s) 196
FaeI CATG 2 cut(s) 282, 426
FaiI YATR 6 cut(s) 190, 192, 280, 284, 302, 424
FatI CATG 2 cut(s) 278, 422
FauNDI CATATG 1 cut(s) 190
Fnu4HI GCNGC 2 cut(s) 50, 276
FokI GGATG 2 cut(s) 108, 185
Fsp4HI GCNGC 2 cut(s) 50, 276
FspBI CTAG 2 cut(s) 111, 243
GluI GCNGC 2 cut(s) 50, 276
HaeIII GGCC 2 cut(s) 49, 365
HapII CCGG 4 cut(s) 53, 61, 90, 116
Hin1II CATG 2 cut(s) 282, 426
HindIII AAGCTT 1 cut(s) 393
HpaII CCGG 4 cut(s) 53, 61, 90, 116
HphI GGTGA 1 cut(s) 80
Hpy166II GTNNAC 1 cut(s) 121
Hpy188I TCNGA 4 cut(s) 18, 26, 270, 351
Hpy8I GTNNAC 1 cut(s) 121
HpyAV CCTTC 3 cut(s) 163, 275, 368
HpyCH4III ACNGT 1 cut(s) 306
HpyCH4V TGCA 3 cut(s) 83, 194, 278
HpyF10VI GCNNNNNNNGC 3 cut(s) 80, 152, 284
Hsp92II CATG 2 cut(s) 282, 426
Kzo9I GATC 2 cut(s) 21, 334
LmnI GCTCC 1 cut(s) 377
LpnPI CCDG 8 cut(s) 66, 74, 103, 129, 170, 331, 339, 379
Lsp1109I GCAGC 1 cut(s) 262
MaeI CTAG 2 cut(s) 111, 243
MaeIII GTNAC 1 cut(s) 98
MalI GATC 2 cut(s) 23, 336
MboI GATC 2 cut(s) 21, 334
MboII GAAGA 3 cut(s) 31, 131, 216
MflI RGATCY 1 cut(s) 21
MluCI AATT 1 cut(s) 12
MmeI TCCRAC 1 cut(s) 253
MnlI CCTC 3 cut(s) 20, 64, 200
Mph1103I ATGCAT 1 cut(s) 196
MroXI GAANNNNTTC 1 cut(s) 264
MseI TTAA 1 cut(s) 197
MslI CAYNNNNRTG 1 cut(s) 421
MspI CCGG 4 cut(s) 53, 61, 90, 116
MspR9I CCNGG 2 cut(s) 61, 90
MwoI GCNNNNNNNGC 3 cut(s) 80, 152, 284
NciI CCSGG 2 cut(s) 61, 90
NdeI CATATG 1 cut(s) 190
NdeII GATC 2 cut(s) 21, 334
NlaIII CATG 2 cut(s) 282, 426
NlaIV GGNNCC 1 cut(s) 364
NmuCI GTSAC 1 cut(s) 98
NsiI ATGCAT 1 cut(s) 196
NspI RCATGY 1 cut(s) 282
PcsI WCGNNNNNNNCGW 1 cut(s) 22
PdmI GAANNNNTTC 1 cut(s) 264
PinAI ACCGGT 1 cut(s) 115
PkrI GCNGC 2 cut(s) 51, 277
PspN4I GGNNCC 1 cut(s) 364
PspPI GGNCC 3 cut(s) 47, 121, 363
PsuI RGATCY 1 cut(s) 21
RseI CAYNNNNRTG 1 cut(s) 421
SaqAI TTAA 1 cut(s) 197
SatI GCNGC 2 cut(s) 50, 276
Sau3AI GATC 2 cut(s) 21, 334
Sau96I GGNCC 3 cut(s) 47, 121, 363
ScrFI CCNGG 2 cut(s) 61, 90
SetI ASST 4 cut(s) 31, 126, 148, 397
SinI GGWCC 1 cut(s) 121
SmiMI CAYNNNNRTG 1 cut(s) 421
Sse9I AATT 1 cut(s) 12
SsiI CCGC 1 cut(s) 50
SspMI CTAG 2 cut(s) 111, 243
StyD4I CCNGG 2 cut(s) 59, 88
TaaI ACNGT 1 cut(s) 306
TaqI TCGA 1 cut(s) 343
TasI AATT 1 cut(s) 12
TauI GCSGC 1 cut(s) 52
Tru1I TTAA 1 cut(s) 197
Tru9I TTAA 1 cut(s) 197
TscAI CASTG 1 cut(s) 333
TseFI GTSAC 1 cut(s) 98
TseI GCWGC 1 cut(s) 275
Tsp45I GTSAC 1 cut(s) 98
TspDTI ATGAA 2 cut(s) 182, 411
TspRI CASTG 1 cut(s) 333
VpaK11BI GGWCC 1 cut(s) 121
XceI RCATGY 1 cut(s) 282
XcmI CCANNNNNNNNNTGG 1 cut(s) 325
XmnI GAANNNNTTC 1 cut(s) 264
XspI CTAG 2 cut(s) 111, 243
Zsp2I ATGCAT 1 cut(s) 196
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.