Rh5DG222900

EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0001544
Physical Location & Seq
Reverse (-)
25057439 .. 25058201
763 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG222900.1

Sequence Viewer

Length: 231 bp
ATGGCCCTGCTAACCACGTGCAATGGTTTAAGTCCTGTAGAAGAGTCTGTATTTGAAAAGCGCATGGCAAATGTAAACGAACTGCGGAAGCTTGGAGCAAAAATTCAAGTCTGTGCAAGCTCTGCTCTGGTTTTCGGGAAAGATAATAGAAGTGGTTTGTCTGGTTCCTGCCTTGCTGCAACTGACCTCAGAGGTGGGATATCATTGGTATTAGCTGCATTGGCTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPSP_synthase

Protein Analysis

76

Amino Acids

7.81

Weight (kDa)

8.61

Isoelectric Point (pI)

44.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPSP_synthase PF00275 2 - 75 1.6e-08 EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 85
AcsI RAATTY 1 cut(s) 102
AcvI CACGTG 1 cut(s) 18
AgsI TTSAA 2 cut(s) 56, 107
AluBI AGCT 3 cut(s) 91, 120, 215
AluI AGCT 3 cut(s) 91, 120, 215
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 3 cut(s) 176, 215, 224
ApoI RAATTY 1 cut(s) 102
AspLEI GCGC 1 cut(s) 63
AspS9I GGNCC 1 cut(s) 4
BbrPI CACGTG 1 cut(s) 18
BbvI GCAGC 3 cut(s) 163, 202, 211
BfmI CTRYAG 1 cut(s) 36
BisI GCNGC 3 cut(s) 177, 216, 225
BlsI GCNGC 3 cut(s) 178, 217, 226
BmgT120I GGNCC 1 cut(s) 4
BmiI GGNNCC 1 cut(s) 166
BsaAI YACGTR 1 cut(s) 18
Bse3DI GCAATG 1 cut(s) 28
BseMI GCAATG 1 cut(s) 28
BseMII CTCAG 1 cut(s) 202
BseXI GCAGC 3 cut(s) 163, 202, 211
BshFI GGCC 1 cut(s) 5
BsnI GGCC 1 cut(s) 5
BspACI CCGC 1 cut(s) 85
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 1 cut(s) 201
BspLI GGNNCC 1 cut(s) 166
BsrDI GCAATG 1 cut(s) 28
Bst6I CTCTTC 1 cut(s) 36
BstAPI GCANNNNNTGC 2 cut(s) 122, 224
BstBAI YACGTR 1 cut(s) 18
BstC8I GCNNGC 1 cut(s) 118
BstDEI CTNAG 1 cut(s) 188
BstHHI GCGC 1 cut(s) 63
BstMWI GCNNNNNNNGC 3 cut(s) 122, 221, 224
BstSFI CTRYAG 1 cut(s) 36
BstV1I GCAGC 3 cut(s) 163, 202, 211
BsuRI GGCC 1 cut(s) 5
Cac8I GCNNGC 1 cut(s) 118
CfoI GCGC 1 cut(s) 63
Cfr13I GGNCC 1 cut(s) 4
CviAII CATG 1 cut(s) 64
CviJI RGCY 5 cut(s) 5, 91, 120, 215, 224
CviKI_1 RGCY 5 cut(s) 5, 91, 120, 215, 224
DdeI CTNAG 1 cut(s) 188
Eam1104I CTCTTC 1 cut(s) 36
EarI CTCTTC 1 cut(s) 36
Eco32I GATATC 1 cut(s) 201
Eco72I CACGTG 1 cut(s) 18
EcoRV GATATC 1 cut(s) 201
FaeI CATG 1 cut(s) 67
FaiI YATR 2 cut(s) 65, 229
FatI CATG 1 cut(s) 63
Fnu4HI GCNGC 3 cut(s) 177, 216, 225
Fsp4HI GCNGC 3 cut(s) 177, 216, 225
GlaI GCGC 1 cut(s) 62
GluI GCNGC 3 cut(s) 177, 216, 225
HaeIII GGCC 1 cut(s) 5
HhaI GCGC 1 cut(s) 63
Hin1II CATG 1 cut(s) 67
Hin6I GCGC 1 cut(s) 61
HinP1I GCGC 1 cut(s) 61
HindIII AAGCTT 1 cut(s) 89
HinfI GANTC 1 cut(s) 44
Hpy166II GTNNAC 1 cut(s) 76
Hpy188I TCNGA 1 cut(s) 191
Hpy188III TCNNGA 1 cut(s) 136
Hpy8I GTNNAC 1 cut(s) 76
HpyCH4IV ACGT 1 cut(s) 17
HpyCH4V TGCA 5 cut(s) 21, 116, 179, 218, 227
HpyF10VI GCNNNNNNNGC 3 cut(s) 122, 221, 224
HpyF3I CTNAG 1 cut(s) 188
HpySE526I ACGT 1 cut(s) 17
Hsp92II CATG 1 cut(s) 67
HspAI GCGC 1 cut(s) 61
LmnI GCTCC 1 cut(s) 95
LpnPI CCDG 5 cut(s) 20, 48, 113, 147, 181
Lsp1109I GCAGC 3 cut(s) 163, 202, 211
MaeII ACGT 1 cut(s) 17
MboII GAAGA 1 cut(s) 53
MluCI AATT 1 cut(s) 102
MlyI GAGTC 1 cut(s) 53
MnlI CCTC 2 cut(s) 185, 197
MseI TTAA 1 cut(s) 29
MwoI GCNNNNNNNGC 3 cut(s) 122, 221, 224
NlaIII CATG 1 cut(s) 67
NlaIV GGNNCC 1 cut(s) 166
PkrI GCNGC 3 cut(s) 178, 217, 226
PleI GAGTC 1 cut(s) 52
PmaCI CACGTG 1 cut(s) 18
PmlI CACGTG 1 cut(s) 18
PpsI GAGTC 1 cut(s) 52
Ppu21I YACGTR 1 cut(s) 18
PspCI CACGTG 1 cut(s) 18
PspN4I GGNNCC 1 cut(s) 166
PspPI GGNCC 1 cut(s) 4
SaqAI TTAA 1 cut(s) 29
SatI GCNGC 3 cut(s) 177, 216, 225
Sau96I GGNCC 1 cut(s) 4
SchI GAGTC 1 cut(s) 53
SetI ASST 6 cut(s) 20, 93, 122, 189, 196, 217
SfcI CTRYAG 1 cut(s) 36
Sse9I AATT 1 cut(s) 102
SsiI CCGC 1 cut(s) 85
TaiI ACGT 1 cut(s) 20
TasI AATT 1 cut(s) 102
Tru1I TTAA 1 cut(s) 29
Tru9I TTAA 1 cut(s) 29
TseI GCWGC 3 cut(s) 176, 215, 224
XapI RAATTY 1 cut(s) 102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.