Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0001544

Physical Location & Seq

Genomic Coordinates Reverse (-)

70375515 .. 70377327

Gene Structure 1813 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000020938

Sequence Viewer

CDS Protein Promoter (2k)

Length: 474 bp

ATGCCATCTAAGGTTGAAGGTATAATGGATGGCAAAGTTCAGGCATATGCATTAAATGGAAGAGGACTAGTTGGGGGAAGTTTCCAACTTGATTACCCTAGCATTGGGGCAACAAAAACCCTTCTAATGGCTGCATGTATGGCTGATGGAACAACTATACAATCCAATGTTGCCAGAGAACCAGAGGTGGTTGATCTTCTTGTCATCAAGGGAAAATCTCATTTGCATGGTTGTAAATGTACTATTGCACCTGATCGTATTGAAGCAGGCACATTTATGCTTGCTGCAGCTATTACTCGCTCATTCATTTCAATTTCACCTGTCATTCCTTCCCAAGTTTCCTGTCTGAAACAAAAACTCTTGGCCGCGGGTTGCAAAATAAGTGGTTTGTCTGGTTCCTGCCTTGCTGCAACTGACCTCAGAGGTGGGGATATCATTGGTATTAGCTGCATTGGCTGCAGAAGGTACTACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EPSP_synthase

Protein Analysis

158

Amino Acids

16.37

Weight (kDa)

8.82

Isoelectric Point (pI)

48.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EPSP_synthase	PF00275	18 - 67	2.5e-06	EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)
EPSP_synthase	PF00275	67 - 128	1.1e-09	EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001544)

Species	Orthologous Gene IDs
malus_domestica	MD03G1241800.v1.1
prunus_persica	Prupe.4G167100_v2.0.a1 Prupe.4G167100_v2.0.a1
pyrus_communis	pycom03g18890
rosa_chinensis	RchiOBHm_Chr5g0030831 RchiOBHm_Chr5g0031211 RchiOBHm_Chr5g0031411 RchiOBHm_Chr7g0194341
rosa_laevigata	RLG00000002889 RLG00000009452 RLG00000010063 RLG00000020938 RLG00000025330 RLG00000029419 RLG00000033292
rosa_multiflora	Rmu_sc0002426.1_g000013 Rmu_sc0002505.1_g000024 Rmu_sc0004790.1_g000012 Rmu_sc0005543.1_g000001 Rmu_sc0014478.1_g000005 Rmu_sc0014478.1_g000006
rosa_roxburghii	Rroxscaffold_1G00039380 Rroxscaffold_1G00049410 Rroxscaffold_4G00315890
rosa_rugosa	Rorug05G0122800 Rorug05G0125100
rosa_samantha	Rh1BG113100 Rh1CG264100 Rh2BG469500 Rh2DG478900 Rh5AG213800 Rh5AG217800 Rh5BG213000 Rh5BG213100 Rh5BG217500 Rh5CG236700 Rh5CG239700 Rh5CG241300 Rh5DG217500 Rh5DG221000 Rh5DG222900 Rh5DG281900 Rh5DG502600 Rh6BG212100 Rh7AG059300 Rh7CG251400
rosa_wichuraiana	Rw0G012990 Rw0G015960 Rw5G019540 Rw5G019900 Rw5G027790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	1 cut(s)	368
AciI	CCGC	2 cut(s)	366, 368
AcoI	YGGCCR	1 cut(s)	363
AfaI	GTAC	2 cut(s)	241, 467
AfiI	CCNNNNNNNGG	2 cut(s)	104, 127
AgsI	TTSAA	3 cut(s)	17, 263, 312
AhlI	ACTAGT	1 cut(s)	67
AluBI	AGCT	2 cut(s)	290, 447
AluI	AGCT	2 cut(s)	290, 447
AoxI	GGCC	1 cut(s)	363
ApeKI	GCWGC	6 cut(s)	131, 284, 287, 407, 447, 456
AsuHPI	GGTGA	1 cut(s)	309
BbvI	GCAGC	6 cut(s)	118, 271, 299, 394, 434, 443
BccI	CCATC	3 cut(s)	13, 23, 140
BcuI	ACTAGT	1 cut(s)	67
BfaI	CTAG	2 cut(s)	68, 99
BfmI	CTRYAG	2 cut(s)	285, 457
BisI	GCNGC	7 cut(s)	132, 285, 288, 366, 408, 448, 457
BlsI	GCNGC	7 cut(s)	133, 286, 289, 367, 409, 449, 458
BmiI	GGNNCC	1 cut(s)	397
BsaJI	CCNNGG	1 cut(s)	366
Bsc4I	CCNNNNNNNGG	2 cut(s)	104, 127
BseDI	CCNNGG	1 cut(s)	366
BseGI	GGATG	1 cut(s)	34
BseLI	CCNNNNNNNGG	2 cut(s)	104, 127
BseMII	CTCAG	1 cut(s)	433
BseXI	GCAGC	6 cut(s)	118, 271, 299, 394, 434, 443
Bsh1236I	CGCG	1 cut(s)	368
BshFI	GGCC	1 cut(s)	365
BslI	CCNNNNNNNGG	2 cut(s)	104, 127
BsnI	GGCC	1 cut(s)	365
Bsp143I	GATC	2 cut(s)	193, 253
BspACI	CCGC	2 cut(s)	366, 368
BspANI	GGCC	1 cut(s)	365
BspCNI	CTCAG	1 cut(s)	432
BspFNI	CGCG	1 cut(s)	368
BspLI	GGNNCC	1 cut(s)	397
BspMAI	CTGCAG	2 cut(s)	289, 461
BssECI	CCNNGG	1 cut(s)	366
BssMI	GATC	2 cut(s)	193, 253
Bst6I	CTCTTC	1 cut(s)	55
BstAPI	GCANNNNNTGC	1 cut(s)	456
BstC8I	GCNNGC	2 cut(s)	268, 282
BstDEI	CTNAG	2 cut(s)	9, 419
BstDSI	CCRYGG	1 cut(s)	366
BstF5I	GGATG	1 cut(s)	34
BstFNI	CGCG	1 cut(s)	368
BstKTI	GATC	2 cut(s)	196, 256
BstMBI	GATC	2 cut(s)	193, 253
BstMWI	GCNNNNNNNGC	3 cut(s)	140, 453, 456
BstNSI	RCATGY	1 cut(s)	138
BstSFI	CTRYAG	2 cut(s)	285, 457
BstUI	CGCG	1 cut(s)	368
BstV1I	GCAGC	6 cut(s)	118, 271, 299, 394, 434, 443
BsuRI	GGCC	1 cut(s)	365
BtgI	CCRYGG	1 cut(s)	366
BtsCI	GGATG	1 cut(s)	34
Cac8I	GCNNGC	2 cut(s)	268, 282
Cfr42I	CCGCGG	1 cut(s)	369
Csp6I	GTAC	2 cut(s)	240, 466
CspCI	CAANNNNNGTGG	2 cut(s)	364, 399
CviAII	CATG	2 cut(s)	135, 227
CviJI	RGCY	6 cut(s)	131, 143, 290, 365, 447, 456
CviKI_1	RGCY	6 cut(s)	131, 143, 290, 365, 447, 456
CviQI	GTAC	2 cut(s)	240, 466
DdeI	CTNAG	2 cut(s)	9, 419
DpnI	GATC	2 cut(s)	195, 255
DpnII	GATC	2 cut(s)	193, 253
EaeI	YGGCCR	1 cut(s)	363
Eam1104I	CTCTTC	1 cut(s)	55
EarI	CTCTTC	1 cut(s)	55
Eco32I	GATATC	1 cut(s)	433
EcoRV	GATATC	1 cut(s)	433
EcoT22I	ATGCAT	1 cut(s)	52
FaeI	CATG	2 cut(s)	138, 230
FaiI	YATR	8 cut(s)	23, 46, 48, 136, 140, 158, 228, 278
FatI	CATG	2 cut(s)	134, 226
FauI	CCCGC	1 cut(s)	361
FauNDI	CATATG	1 cut(s)	46
Fnu4HI	GCNGC	7 cut(s)	132, 285, 288, 366, 408, 448, 457
FokI	GGATG	1 cut(s)	41
Fsp4HI	GCNGC	7 cut(s)	132, 285, 288, 366, 408, 448, 457
FspBI	CTAG	2 cut(s)	68, 99
GluI	GCNGC	7 cut(s)	132, 285, 288, 366, 408, 448, 457
HaeIII	GGCC	1 cut(s)	365
Hin1II	CATG	2 cut(s)	138, 230
HphI	GGTGA	1 cut(s)	309
Hpy188I	TCNGA	2 cut(s)	348, 422
HpyAV	CCTTC	4 cut(s)	11, 131, 339, 456
HpyCH4V	TGCA	9 cut(s)	50, 134, 226, 248, 287, 375, 410, 450, 459
HpyF10VI	GCNNNNNNNGC	3 cut(s)	140, 453, 456
HpyF3I	CTNAG	2 cut(s)	9, 419
Hsp92II	CATG	2 cut(s)	138, 230
KspI	CCGCGG	1 cut(s)	369
Kzo9I	GATC	2 cut(s)	193, 253
LpnPI	CCDG	9 cut(s)	26, 187, 195, 252, 264, 333, 355, 378, 412
Lsp1109I	GCAGC	6 cut(s)	118, 271, 299, 394, 434, 443
MaeI	CTAG	2 cut(s)	68, 99
MalI	GATC	2 cut(s)	195, 255
MboI	GATC	2 cut(s)	193, 253
MboII	GAAGA	2 cut(s)	72, 188
MluCI	AATT	1 cut(s)	312
MmeI	TCCRAC	1 cut(s)	109
MnlI	CCTC	4 cut(s)	56, 178, 416, 428
Mph1103I	ATGCAT	1 cut(s)	52
MseI	TTAA	1 cut(s)	53
MslI	CAYNNNNRTG	2 cut(s)	225, 275
MspA1I	CMGCKG	1 cut(s)	368
MvnI	CGCG	1 cut(s)	368
MwoI	GCNNNNNNNGC	3 cut(s)	140, 453, 456
NdeI	CATATG	1 cut(s)	46
NdeII	GATC	2 cut(s)	193, 253
NlaIII	CATG	2 cut(s)	138, 230
NlaIV	GGNNCC	1 cut(s)	397
NsiI	ATGCAT	1 cut(s)	52
NspI	RCATGY	1 cut(s)	138
PkrI	GCNGC	7 cut(s)	133, 286, 289, 367, 409, 449, 458
PspN4I	GGNNCC	1 cut(s)	397
PstI	CTGCAG	2 cut(s)	289, 461
RsaI	GTAC	2 cut(s)	241, 467
RsaNI	GTAC	2 cut(s)	240, 466
RseI	CAYNNNNRTG	2 cut(s)	225, 275
SacII	CCGCGG	1 cut(s)	369
SaqAI	TTAA	1 cut(s)	53
SatI	GCNGC	7 cut(s)	132, 285, 288, 366, 408, 448, 457
Sau3AI	GATC	2 cut(s)	193, 253
SfcI	CTRYAG	2 cut(s)	285, 457
Sfr303I	CCGCGG	1 cut(s)	369
SgrBI	CCGCGG	1 cut(s)	369
SmiMI	CAYNNNNRTG	2 cut(s)	225, 275
SpeI	ACTAGT	1 cut(s)	67
Sse9I	AATT	1 cut(s)	312
SsiI	CCGC	2 cut(s)	366, 368
SspMI	CTAG	2 cut(s)	68, 99
TasI	AATT	1 cut(s)	312
TatI	WGTACW	1 cut(s)	239
TauI	GCSGC	1 cut(s)	368
Tru1I	TTAA	1 cut(s)	53
Tru9I	TTAA	1 cut(s)	53
TseI	GCWGC	6 cut(s)	131, 284, 287, 407, 447, 456
TspDTI	ATGAA	1 cut(s)	295
XceI	RCATGY	1 cut(s)	138
XspI	CTAG	2 cut(s)	68, 99
Zsp2I	ATGCAT	1 cut(s)	52

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.