Rh5CG239700

EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0001544
Physical Location & Seq
Reverse (-)
24847732 .. 24848619
888 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG239700.1

Sequence Viewer

Length: 348 bp
ATGGCCCTGCTAACCACGTGCAATGGTTTAAGTCCCGTAGAAGAGTCTGTATTTGAAAAGCGCATGGCAAATGTAAACGAATTGCGGAAGCTTGGAGCAAAAATTCAAGTCTGTGCAAGCTCTACTCTGGTTTTCGGGAAAGATAATAGAAGTGGTTTGTCTGGTTCCTGCCTTGCTGCAACTGACCTCAGAGGTGGGATATCATTGGTATTAGCTGCATTGGCTGCAGAAGGTACTACTGAGATCAGCAGTGTTGCTCATATTGACCGTGGTTATGAGAATGTAGATACACAACTTCATATGCTGGGAGCCGATATCAAAAGATTAACGGCCCCTGCTTCTTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPSP_synthase

Protein Analysis

115

Amino Acids

12.01

Weight (kDa)

6.07

Isoelectric Point (pI)

39.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPSP_synthase PF00275 2 - 95 3.5e-15 EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 85
AcsI RAATTY 1 cut(s) 102
AcvI CACGTG 1 cut(s) 18
AfaI GTAC 1 cut(s) 235
AgsI TTSAA 2 cut(s) 56, 107
AluBI AGCT 3 cut(s) 91, 120, 215
AluI AGCT 3 cut(s) 91, 120, 215
AoxI GGCC 2 cut(s) 3, 330
ApeKI GCWGC 3 cut(s) 176, 215, 224
ApoI RAATTY 1 cut(s) 102
AspLEI GCGC 1 cut(s) 63
AspS9I GGNCC 2 cut(s) 4, 331
BbrPI CACGTG 1 cut(s) 18
BbvI GCAGC 3 cut(s) 163, 202, 211
BceAI ACGGC 1 cut(s) 345
BfaI CTAG 1 cut(s) 346
BfmI CTRYAG 1 cut(s) 225
BisI GCNGC 3 cut(s) 177, 216, 225
BlsI GCNGC 3 cut(s) 178, 217, 226
BmgT120I GGNCC 2 cut(s) 4, 331
BmiI GGNNCC 3 cut(s) 166, 310, 333
BsaAI YACGTR 1 cut(s) 18
BsaJI CCNNGG 1 cut(s) 268
Bse3DI GCAATG 1 cut(s) 28
BseDI CCNNGG 1 cut(s) 268
BseMI GCAATG 1 cut(s) 28
BseMII CTCAG 2 cut(s) 202, 231
BseXI GCAGC 3 cut(s) 163, 202, 211
BseYI CCCAGC 1 cut(s) 304
BshFI GGCC 2 cut(s) 5, 332
BslFI GGGAC 1 cut(s) 18
BsmFI GGGAC 1 cut(s) 18
BsnI GGCC 2 cut(s) 5, 332
Bsp143I GATC 1 cut(s) 243
BspACI CCGC 1 cut(s) 85
BspANI GGCC 2 cut(s) 5, 332
BspCNI CTCAG 2 cut(s) 201, 232
BspLI GGNNCC 3 cut(s) 166, 310, 333
BspMAI CTGCAG 1 cut(s) 229
BsrDI GCAATG 1 cut(s) 28
BssECI CCNNGG 1 cut(s) 268
BssMI GATC 1 cut(s) 243
Bst4CI ACNGT 1 cut(s) 269
Bst6I CTCTTC 1 cut(s) 36
BstAPI GCANNNNNTGC 1 cut(s) 224
BstBAI YACGTR 1 cut(s) 18
BstC8I GCNNGC 1 cut(s) 118
BstDEI CTNAG 2 cut(s) 188, 240
BstDSI CCRYGG 1 cut(s) 268
BstHHI GCGC 1 cut(s) 63
BstKTI GATC 1 cut(s) 246
BstMBI GATC 1 cut(s) 243
BstMWI GCNNNNNNNGC 2 cut(s) 221, 224
BstSFI CTRYAG 1 cut(s) 225
BstV1I GCAGC 3 cut(s) 163, 202, 211
BsuRI GGCC 2 cut(s) 5, 332
BtgI CCRYGG 1 cut(s) 268
BtsI GCAGTG 1 cut(s) 256
BtsIMutI CAGTG 1 cut(s) 256
Cac8I GCNNGC 1 cut(s) 118
CfoI GCGC 1 cut(s) 63
Cfr13I GGNCC 2 cut(s) 4, 331
Csp6I GTAC 1 cut(s) 234
CviAII CATG 1 cut(s) 64
CviJI RGCY 7 cut(s) 5, 91, 120, 215, 224, 311, 332
CviKI_1 RGCY 7 cut(s) 5, 91, 120, 215, 224, 311, 332
CviQI GTAC 1 cut(s) 234
DdeI CTNAG 2 cut(s) 188, 240
DpnI GATC 1 cut(s) 245
DpnII GATC 1 cut(s) 243
Eam1104I CTCTTC 1 cut(s) 36
EarI CTCTTC 1 cut(s) 36
Eco32I GATATC 2 cut(s) 201, 316
Eco72I CACGTG 1 cut(s) 18
EcoRV GATATC 2 cut(s) 201, 316
FaeI CATG 1 cut(s) 67
FaiI YATR 5 cut(s) 65, 261, 276, 300, 302
FaqI GGGAC 1 cut(s) 18
FatI CATG 1 cut(s) 63
FauNDI CATATG 1 cut(s) 300
Fnu4HI GCNGC 3 cut(s) 177, 216, 225
Fsp4HI GCNGC 3 cut(s) 177, 216, 225
FspBI CTAG 1 cut(s) 346
GlaI GCGC 1 cut(s) 62
GluI GCNGC 3 cut(s) 177, 216, 225
GsaI CCCAGC 1 cut(s) 308
HaeIII GGCC 2 cut(s) 5, 332
HhaI GCGC 1 cut(s) 63
Hin1II CATG 1 cut(s) 67
Hin6I GCGC 1 cut(s) 61
HinP1I GCGC 1 cut(s) 61
HindIII AAGCTT 1 cut(s) 89
HinfI GANTC 1 cut(s) 44
Hpy166II GTNNAC 1 cut(s) 76
Hpy188I TCNGA 1 cut(s) 191
Hpy188III TCNNGA 1 cut(s) 136
Hpy8I GTNNAC 1 cut(s) 76
HpyAV CCTTC 1 cut(s) 224
HpyCH4III ACNGT 1 cut(s) 269
HpyCH4IV ACGT 1 cut(s) 17
HpyCH4V TGCA 5 cut(s) 21, 116, 179, 218, 227
HpyF10VI GCNNNNNNNGC 2 cut(s) 221, 224
HpyF3I CTNAG 2 cut(s) 188, 240
HpySE526I ACGT 1 cut(s) 17
Hsp92II CATG 1 cut(s) 67
HspAI GCGC 1 cut(s) 61
Kzo9I GATC 1 cut(s) 243
LmnI GCTCC 2 cut(s) 95, 308
LpnPI CCDG 5 cut(s) 20, 113, 147, 181, 290
Lsp1109I GCAGC 3 cut(s) 163, 202, 211
MaeI CTAG 1 cut(s) 346
MaeII ACGT 1 cut(s) 17
MalI GATC 1 cut(s) 245
MboI GATC 1 cut(s) 243
MboII GAAGA 2 cut(s) 53, 333
MluCI AATT 2 cut(s) 80, 102
MlyI GAGTC 1 cut(s) 53
MnlI CCTC 2 cut(s) 185, 197
MseI TTAA 2 cut(s) 29, 326
MwoI GCNNNNNNNGC 2 cut(s) 221, 224
NdeI CATATG 1 cut(s) 300
NdeII GATC 1 cut(s) 243
NlaIII CATG 1 cut(s) 67
NlaIV GGNNCC 3 cut(s) 166, 310, 333
PkrI GCNGC 3 cut(s) 178, 217, 226
PleI GAGTC 1 cut(s) 52
PmaCI CACGTG 1 cut(s) 18
PmlI CACGTG 1 cut(s) 18
PpsI GAGTC 1 cut(s) 52
Ppu21I YACGTR 1 cut(s) 18
PspCI CACGTG 1 cut(s) 18
PspFI CCCAGC 1 cut(s) 304
PspN4I GGNNCC 3 cut(s) 166, 310, 333
PspPI GGNCC 2 cut(s) 4, 331
PstI CTGCAG 1 cut(s) 229
RsaI GTAC 1 cut(s) 235
RsaNI GTAC 1 cut(s) 234
SaqAI TTAA 2 cut(s) 29, 326
SatI GCNGC 3 cut(s) 177, 216, 225
Sau3AI GATC 1 cut(s) 243
Sau96I GGNCC 2 cut(s) 4, 331
SchI GAGTC 1 cut(s) 53
SetI ASST 7 cut(s) 20, 93, 122, 189, 196, 217, 235
SfcI CTRYAG 1 cut(s) 225
Sse9I AATT 2 cut(s) 80, 102
SsiI CCGC 1 cut(s) 85
SspMI CTAG 1 cut(s) 346
TaaI ACNGT 1 cut(s) 269
TaiI ACGT 1 cut(s) 20
TasI AATT 2 cut(s) 80, 102
Tru1I TTAA 2 cut(s) 29, 326
Tru9I TTAA 2 cut(s) 29, 326
TscAI CASTG 1 cut(s) 256
TseI GCWGC 3 cut(s) 176, 215, 224
TspDTI ATGAA 1 cut(s) 287
TspRI CASTG 1 cut(s) 256
XapI RAATTY 1 cut(s) 102
XspI CTAG 1 cut(s) 346
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.