Rw5G019900

EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0001544
Physical Location & Seq
Reverse (-)
25968707 .. 25969582
876 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G019900.1

Sequence Viewer

Length: 348 bp
ATGGCCCTGCTAACCACATGCAATGGTTTAAGTCCTGTAGAAGAGTCTGTATTTGAAAAGCGCATGGCAAAAGTAAACGAACTGCGGAAGCTTGGAGCAAAAATTCAAGTCTGTGCAAGCTCTACTCTGGTTTTTGGGAAAGATAATAGAAGTGGTTTGTCTGGTTCCTGCCTTGCTGCAACTGACCTTAGAGGTGGGATATCATTGGTATTAGCTGCATTGGCTGCAGAAGGTACTACTGAGATCAGCAGTGTTGCCCATATTGACCGTGGTTATGAGAATGTAGATACACAACTTCATATGCTGGGAGCCGATATCAAAAGATTAACAGCCCCTGCTTCTTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPSP_synthase

Protein Analysis

115

Amino Acids

12.02

Weight (kDa)

6.71

Isoelectric Point (pI)

39.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPSP_synthase PF00275 2 - 95 3.9e-15 EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 85
AcsI RAATTY 1 cut(s) 102
AfaI GTAC 1 cut(s) 235
AgsI TTSAA 2 cut(s) 56, 107
AluBI AGCT 3 cut(s) 91, 120, 215
AluI AGCT 3 cut(s) 91, 120, 215
AlwNI CAGNNNCTG 1 cut(s) 335
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 3 cut(s) 176, 215, 224
ApoI RAATTY 1 cut(s) 102
AspLEI GCGC 1 cut(s) 63
AspS9I GGNCC 1 cut(s) 4
BbvI GCAGC 3 cut(s) 163, 202, 211
BfaI CTAG 1 cut(s) 346
BfmI CTRYAG 2 cut(s) 36, 225
BisI GCNGC 3 cut(s) 177, 216, 225
BlsI GCNGC 3 cut(s) 178, 217, 226
BmgT120I GGNCC 1 cut(s) 4
BmiI GGNNCC 2 cut(s) 166, 310
BsaJI CCNNGG 1 cut(s) 268
Bse3DI GCAATG 1 cut(s) 28
BseDI CCNNGG 1 cut(s) 268
BseMI GCAATG 1 cut(s) 28
BseMII CTCAG 1 cut(s) 231
BseXI GCAGC 3 cut(s) 163, 202, 211
BseYI CCCAGC 1 cut(s) 304
BshFI GGCC 1 cut(s) 5
BsnI GGCC 1 cut(s) 5
Bsp143I GATC 1 cut(s) 243
BspACI CCGC 1 cut(s) 85
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 1 cut(s) 232
BspLI GGNNCC 2 cut(s) 166, 310
BspMAI CTGCAG 1 cut(s) 229
BsrDI GCAATG 1 cut(s) 28
BssECI CCNNGG 1 cut(s) 268
BssMI GATC 1 cut(s) 243
Bst4CI ACNGT 1 cut(s) 269
Bst6I CTCTTC 1 cut(s) 36
BstAPI GCANNNNNTGC 1 cut(s) 224
BstC8I GCNNGC 1 cut(s) 118
BstDEI CTNAG 2 cut(s) 188, 240
BstDSI CCRYGG 1 cut(s) 268
BstHHI GCGC 1 cut(s) 63
BstKTI GATC 1 cut(s) 246
BstMBI GATC 1 cut(s) 243
BstMWI GCNNNNNNNGC 2 cut(s) 221, 224
BstNSI RCATGY 1 cut(s) 21
BstSFI CTRYAG 2 cut(s) 36, 225
BstV1I GCAGC 3 cut(s) 163, 202, 211
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 268
BtsI GCAGTG 1 cut(s) 256
BtsIMutI CAGTG 1 cut(s) 256
Cac8I GCNNGC 1 cut(s) 118
CaiI CAGNNNCTG 1 cut(s) 335
CfoI GCGC 1 cut(s) 63
Cfr13I GGNCC 1 cut(s) 4
Csp6I GTAC 1 cut(s) 234
CviAII CATG 2 cut(s) 18, 64
CviJI RGCY 7 cut(s) 5, 91, 120, 215, 224, 311, 332
CviKI_1 RGCY 7 cut(s) 5, 91, 120, 215, 224, 311, 332
CviQI GTAC 1 cut(s) 234
DdeI CTNAG 2 cut(s) 188, 240
DpnI GATC 1 cut(s) 245
DpnII GATC 1 cut(s) 243
Eam1104I CTCTTC 1 cut(s) 36
EarI CTCTTC 1 cut(s) 36
Eco32I GATATC 2 cut(s) 201, 316
EcoRV GATATC 2 cut(s) 201, 316
FaeI CATG 2 cut(s) 21, 67
FaiI YATR 6 cut(s) 19, 65, 261, 276, 300, 302
FatI CATG 2 cut(s) 17, 63
FauNDI CATATG 1 cut(s) 300
Fnu4HI GCNGC 3 cut(s) 177, 216, 225
Fsp4HI GCNGC 3 cut(s) 177, 216, 225
FspBI CTAG 1 cut(s) 346
GlaI GCGC 1 cut(s) 62
GluI GCNGC 3 cut(s) 177, 216, 225
GsaI CCCAGC 1 cut(s) 308
HaeIII GGCC 1 cut(s) 5
HhaI GCGC 1 cut(s) 63
Hin1II CATG 2 cut(s) 21, 67
Hin6I GCGC 1 cut(s) 61
HinP1I GCGC 1 cut(s) 61
HindIII AAGCTT 1 cut(s) 89
HinfI GANTC 1 cut(s) 44
Hpy166II GTNNAC 1 cut(s) 76
Hpy8I GTNNAC 1 cut(s) 76
HpyAV CCTTC 1 cut(s) 224
HpyCH4III ACNGT 1 cut(s) 269
HpyCH4V TGCA 5 cut(s) 21, 116, 179, 218, 227
HpyF10VI GCNNNNNNNGC 2 cut(s) 221, 224
HpyF3I CTNAG 2 cut(s) 188, 240
Hsp92II CATG 2 cut(s) 21, 67
HspAI GCGC 1 cut(s) 61
Kzo9I GATC 1 cut(s) 243
LmnI GCTCC 2 cut(s) 95, 308
LpnPI CCDG 6 cut(s) 20, 48, 113, 147, 181, 290
Lsp1109I GCAGC 3 cut(s) 163, 202, 211
MaeI CTAG 1 cut(s) 346
MalI GATC 1 cut(s) 245
MboI GATC 1 cut(s) 243
MboII GAAGA 2 cut(s) 53, 333
MluCI AATT 1 cut(s) 102
MlyI GAGTC 1 cut(s) 53
MnlI CCTC 1 cut(s) 185
MseI TTAA 2 cut(s) 29, 326
MwoI GCNNNNNNNGC 2 cut(s) 221, 224
NdeI CATATG 1 cut(s) 300
NdeII GATC 1 cut(s) 243
NlaIII CATG 2 cut(s) 21, 67
NlaIV GGNNCC 2 cut(s) 166, 310
NspI RCATGY 1 cut(s) 21
PkrI GCNGC 3 cut(s) 178, 217, 226
PleI GAGTC 1 cut(s) 52
PpsI GAGTC 1 cut(s) 52
PspFI CCCAGC 1 cut(s) 304
PspN4I GGNNCC 2 cut(s) 166, 310
PspPI GGNCC 1 cut(s) 4
PstI CTGCAG 1 cut(s) 229
PstNI CAGNNNCTG 1 cut(s) 335
RsaI GTAC 1 cut(s) 235
RsaNI GTAC 1 cut(s) 234
SaqAI TTAA 2 cut(s) 29, 326
SatI GCNGC 3 cut(s) 177, 216, 225
Sau3AI GATC 1 cut(s) 243
Sau96I GGNCC 1 cut(s) 4
SchI GAGTC 1 cut(s) 53
SetI ASST 6 cut(s) 93, 122, 189, 196, 217, 235
SfcI CTRYAG 2 cut(s) 36, 225
Sse9I AATT 1 cut(s) 102
SsiI CCGC 1 cut(s) 85
SspMI CTAG 1 cut(s) 346
TaaI ACNGT 1 cut(s) 269
TasI AATT 1 cut(s) 102
Tru1I TTAA 2 cut(s) 29, 326
Tru9I TTAA 2 cut(s) 29, 326
TscAI CASTG 1 cut(s) 256
TseI GCWGC 3 cut(s) 176, 215, 224
TspDTI ATGAA 1 cut(s) 287
TspRI CASTG 1 cut(s) 256
XapI RAATTY 1 cut(s) 102
XceI RCATGY 1 cut(s) 21
XcmI CCANNNNNNNNNTGG 1 cut(s) 266
XspI CTAG 1 cut(s) 346
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.