Rh6BG212100

EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0001544
Physical Location & Seq
Reverse (-)
40017738 .. 40021242
3505 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG212100.1

Sequence Viewer

Length: 681 bp
ATGTTTGACTCGACGAGCAGGCAAGGTGACGATGGCAATAAGCTATTGTGGCTGGACAGGGTGACCGGAAGGGTGTGCACGTCGATTTCTGCAAAGGCCATGGCTATAACAGGGATTGAGGATCGGCGATACTGGAAGAATTGGGGCACATTTATGCTTGCTGCAGCTATTACTCGCTCATTCATTTCAATTTCACCTGTCATTCCTTCCCAAGTTTCCTGTCTGATGCAAAAACTCTTGGCCGCTGGTTGCAAAATAAGGCAATGCTCTCATGATACCTTGGAAGTTTCAGCAGTGTCTCGATGTGGTGAGAATTTGCGAGGTTTTGAGGTTAAGACGGGGCCATTTCCCGGGTTTCCTACAGATCTCCAACCGCAAACAATGGCACTGCTAGCCACTTGGTTTAAGTCCTGTAGAAGTCTGTATTTGAAAAGCGCATGGCTCATGAAGCTTGGAGCAAAAATTCAAGTCTGTGCAAGCTCTGATAATAGAAATGGTTTGTCTGGTTCCTGCCTTGCTGTAACTGACCTCAGAGGTGGGATATCATTGGCATTGGCCGCAGAAGGTGCTACTGAGATCAGCGGTGTTGCTCATATTGACCGTGGTTATGAGAATGTAGATACAAAAGTTCATATGCTGGGAGCCGATATCAAAAGATTAACGGCCCCTGCTTCTACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPSP_synthase

Protein Analysis

226

Amino Acids

24.41

Weight (kDa)

9.08

Isoelectric Point (pI)

38.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPSP_synthase PF00275 49 - 206 6.9e-13 EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 243, 374, 558, 582
AclWI GGATC 1 cut(s) 129
AcoI YGGCCR 2 cut(s) 240, 555
AcsI RAATTY 2 cut(s) 313, 462
AfiI CCNNNNNNNGG 1 cut(s) 350
AgsI TTSAA 3 cut(s) 189, 430, 467
AjiI CACGTC 1 cut(s) 81
AluBI AGCT 4 cut(s) 43, 167, 451, 480
AluI AGCT 4 cut(s) 43, 167, 451, 480
Alw21I GWGCWC 1 cut(s) 80
Alw26I GTCTC 1 cut(s) 303
Alw44I GTGCAC 1 cut(s) 76
AlwI GGATC 1 cut(s) 129
Ama87I CYCGRG 1 cut(s) 350
AoxI GGCC 5 cut(s) 96, 240, 341, 555, 663
ApaLI GTGCAC 1 cut(s) 76
ApeKI GCWGC 2 cut(s) 161, 164
ApoI RAATTY 2 cut(s) 313, 462
AspLEI GCGC 1 cut(s) 437
AspS9I GGNCC 2 cut(s) 341, 664
AsuC2I CCSGG 2 cut(s) 351, 352
AsuHPI GGTGA 4 cut(s) 38, 73, 186, 320
AsuNHI GCTAGC 1 cut(s) 391
AvaI CYCGRG 1 cut(s) 350
BaeGI GKGCMC 2 cut(s) 80, 149
BaeI ACNNNNGTAYC 2 cut(s) 612, 645
Bbv12I GWGCWC 1 cut(s) 80
BbvI GCAGC 2 cut(s) 148, 176
BccI CCATC 1 cut(s) 26
BceAI ACGGC 1 cut(s) 678
BcnI CCSGG 2 cut(s) 351, 352
BcoDI GTCTC 1 cut(s) 303
BfaI CTAG 2 cut(s) 392, 679
BfmI CTRYAG 3 cut(s) 162, 360, 412
BglII AGATCT 1 cut(s) 364
BisI GCNGC 4 cut(s) 162, 165, 243, 558
BlsI GCNGC 4 cut(s) 163, 166, 244, 559
Bme1390I CCNGG 2 cut(s) 351, 352
BmeT110I CYCGRG 1 cut(s) 350
BmgBI CACGTC 1 cut(s) 81
BmgT120I GGNCC 2 cut(s) 341, 664
BmiI GGNNCC 4 cut(s) 342, 508, 643, 666
BmrFI CCNGG 2 cut(s) 351, 352
BmsI GCATC 1 cut(s) 216
BmtI GCTAGC 1 cut(s) 395
BpuMI CCSGG 2 cut(s) 351, 352
BsaJI CCNNGG 4 cut(s) 99, 279, 350, 601
BsaWI WCCGGW 1 cut(s) 65
Bsc4I CCNNNNNNNGG 1 cut(s) 350
Bse1I ACTGG 1 cut(s) 137
Bse3DI GCAATG 1 cut(s) 269
BseDI CCNNGG 4 cut(s) 99, 279, 350, 601
BseLI CCNNNNNNNGG 1 cut(s) 350
BseMI GCAATG 1 cut(s) 269
BseMII CTCAG 2 cut(s) 544, 564
BseNI ACTGG 1 cut(s) 137
BseSI GKGCMC 2 cut(s) 80, 149
BseXI GCAGC 2 cut(s) 148, 176
BseYI CCCAGC 1 cut(s) 637
BshFI GGCC 5 cut(s) 98, 242, 343, 557, 665
BsiHKAI GWGCWC 1 cut(s) 80
BsiHKCI CYCGRG 1 cut(s) 350
BsiSI CCGG 2 cut(s) 66, 351
BslI CCNNNNNNNGG 1 cut(s) 350
BsmAI GTCTC 1 cut(s) 303
BsnI GGCC 5 cut(s) 98, 242, 343, 557, 665
BsoBI CYCGRG 1 cut(s) 350
Bsp1286I GDGCHC 2 cut(s) 80, 149
Bsp143I GATC 3 cut(s) 121, 364, 576
Bsp19I CCATGG 1 cut(s) 99
BspACI CCGC 4 cut(s) 243, 374, 558, 582
BspANI GGCC 5 cut(s) 98, 242, 343, 557, 665
BspCNI CTCAG 2 cut(s) 543, 565
BspHI TCATGA 2 cut(s) 271, 444
BspLI GGNNCC 4 cut(s) 342, 508, 643, 666
BspMAI CTGCAG 1 cut(s) 166
BspOI GCTAGC 1 cut(s) 395
BspPI GGATC 1 cut(s) 129
BsrDI GCAATG 1 cut(s) 269
BsrI ACTGG 1 cut(s) 137
BssECI CCNNGG 4 cut(s) 99, 279, 350, 601
BssMI GATC 3 cut(s) 121, 364, 576
BssT1I CCWWGG 2 cut(s) 99, 279
Bst4CI ACNGT 1 cut(s) 602
BstAPI GCANNNNNTGC 1 cut(s) 566
BstC8I GCNNGC 4 cut(s) 20, 159, 393, 478
BstDEI CTNAG 2 cut(s) 530, 573
BstDSI CCRYGG 2 cut(s) 99, 601
BstEII GGTNACC 1 cut(s) 61
BstHHI GCGC 1 cut(s) 437
BstKTI GATC 3 cut(s) 124, 367, 579
BstMAI GTCTC 1 cut(s) 303
BstMBI GATC 3 cut(s) 121, 364, 576
BstMWI GCNNNNNNNGC 5 cut(s) 49, 392, 448, 557, 566
BstPI GGTNACC 1 cut(s) 61
BstSCI CCNGG 2 cut(s) 349, 350
BstSFI CTRYAG 3 cut(s) 162, 360, 412
BstSLI GKGCMC 2 cut(s) 80, 149
BstV1I GCAGC 2 cut(s) 148, 176
BstX2I RGATCY 1 cut(s) 364
BstYI RGATCY 1 cut(s) 364
BsuRI GGCC 5 cut(s) 98, 242, 343, 557, 665
BtgI CCRYGG 2 cut(s) 99, 601
BtrI CACGTC 1 cut(s) 81
BtsI GCAGTG 2 cut(s) 300, 386
BtsIMutI CAGTG 2 cut(s) 300, 386
Cac8I GCNNGC 4 cut(s) 20, 159, 393, 478
CciI TCATGA 2 cut(s) 271, 444
CfoI GCGC 1 cut(s) 437
Cfr13I GGNCC 2 cut(s) 341, 664
Cfr9I CCCGGG 1 cut(s) 350
CviAII CATG 4 cut(s) 100, 272, 438, 445
DdeI CTNAG 2 cut(s) 530, 573
DpnI GATC 3 cut(s) 123, 366, 578
DpnII GATC 3 cut(s) 121, 364, 576
EaeI YGGCCR 2 cut(s) 240, 555
Eco130I CCWWGG 2 cut(s) 99, 279
Eco32I GATATC 2 cut(s) 543, 649
Eco88I CYCGRG 1 cut(s) 350
Eco91I GGTNACC 1 cut(s) 61
EcoO65I GGTNACC 1 cut(s) 61
EcoRV GATATC 2 cut(s) 543, 649
EcoT14I CCWWGG 2 cut(s) 99, 279
ErhI CCWWGG 2 cut(s) 99, 279
FaeI CATG 4 cut(s) 103, 275, 441, 448
FatI CATG 4 cut(s) 99, 271, 437, 444
FauNDI CATATG 1 cut(s) 633
Fnu4HI GCNGC 4 cut(s) 162, 165, 243, 558
Fsp4HI GCNGC 4 cut(s) 162, 165, 243, 558
FspBI CTAG 2 cut(s) 392, 679
GlaI GCGC 1 cut(s) 436
GluI GCNGC 4 cut(s) 162, 165, 243, 558
GsaI CCCAGC 1 cut(s) 641
HaeIII GGCC 5 cut(s) 98, 242, 343, 557, 665
HapII CCGG 2 cut(s) 66, 351
HhaI GCGC 1 cut(s) 437
Hin1II CATG 4 cut(s) 103, 275, 441, 448
Hin6I GCGC 1 cut(s) 435
HinP1I GCGC 1 cut(s) 435
HindIII AAGCTT 1 cut(s) 449
HinfI GANTC 1 cut(s) 8
HpaII CCGG 2 cut(s) 66, 351
HphI GGTGA 4 cut(s) 38, 73, 186, 320
Hpy166II GTNNAC 1 cut(s) 78
Hpy188I TCNGA 3 cut(s) 225, 484, 533
Hpy188III TCNNGA 3 cut(s) 272, 300, 445
Hpy8I GTNNAC 1 cut(s) 78
Hpy99I CGWCG 2 cut(s) 16, 85
HpyAV CCTTC 3 cut(s) 63, 216, 557
HpyCH4III ACNGT 1 cut(s) 602
HpyCH4IV ACGT 1 cut(s) 80
HpyCH4V TGCA 6 cut(s) 78, 92, 164, 229, 252, 476
HpyF10VI GCNNNNNNNGC 5 cut(s) 49, 392, 448, 557, 566
HpyF3I CTNAG 2 cut(s) 530, 573
HpySE526I ACGT 1 cut(s) 80
Hsp92II CATG 4 cut(s) 103, 275, 441, 448
HspAI GCGC 1 cut(s) 435
Kzo9I GATC 3 cut(s) 121, 364, 576
LmnI GCTCC 2 cut(s) 455, 641
Lsp1109I GCAGC 2 cut(s) 148, 176
LweI GCATC 1 cut(s) 216
MaeI CTAG 2 cut(s) 392, 679
MaeII ACGT 1 cut(s) 80
MaeIII GTNAC 3 cut(s) 26, 61, 520
MalI GATC 3 cut(s) 123, 366, 578
MboI GATC 3 cut(s) 121, 364, 576
MboII GAAGA 1 cut(s) 148
MflI RGATCY 1 cut(s) 364
MhlI GDGCHC 2 cut(s) 80, 149
MluCI AATT 4 cut(s) 139, 189, 313, 462
MlyI GAGTC 1 cut(s) 2
MmeI TCCRAC 1 cut(s) 394
MnlI CCTC 5 cut(s) 112, 314, 322, 527, 539
MseI TTAA 3 cut(s) 333, 405, 659
MslI CAYNNNNRTG 1 cut(s) 152
MspA1I CMGCKG 2 cut(s) 245, 582
MspI CCGG 2 cut(s) 66, 351
MspR9I CCNGG 2 cut(s) 351, 352
MwoI GCNNNNNNNGC 5 cut(s) 49, 392, 448, 557, 566
NciI CCSGG 2 cut(s) 351, 352
NcoI CCATGG 1 cut(s) 99
NdeI CATATG 1 cut(s) 633
NdeII GATC 3 cut(s) 121, 364, 576
NheI GCTAGC 1 cut(s) 391
NlaIII CATG 4 cut(s) 103, 275, 441, 448
NlaIV GGNNCC 4 cut(s) 342, 508, 643, 666
NmuCI GTSAC 2 cut(s) 26, 61
PagI TCATGA 2 cut(s) 271, 444
PkrI GCNGC 4 cut(s) 163, 166, 244, 559
PleI GAGTC 1 cut(s) 2
PpsI GAGTC 1 cut(s) 2
PspEI GGTNACC 1 cut(s) 61
PspFI CCCAGC 1 cut(s) 637
PspN4I GGNNCC 4 cut(s) 342, 508, 643, 666
PspPI GGNCC 2 cut(s) 341, 664
PstI CTGCAG 1 cut(s) 166
PsuI RGATCY 1 cut(s) 364
RseI CAYNNNNRTG 1 cut(s) 152
SaqAI TTAA 3 cut(s) 333, 405, 659
SatI GCNGC 4 cut(s) 162, 165, 243, 558
Sau3AI GATC 3 cut(s) 121, 364, 576
Sau96I GGNCC 2 cut(s) 341, 664
SchI GAGTC 1 cut(s) 2
ScrFI CCNGG 2 cut(s) 351, 352
SduI GDGCHC 2 cut(s) 80, 149
SfaNI GCATC 1 cut(s) 216
SfcI CTRYAG 3 cut(s) 162, 360, 412
SmaI CCCGGG 1 cut(s) 352
SmiMI CAYNNNNRTG 1 cut(s) 152
Sse9I AATT 4 cut(s) 139, 189, 313, 462
SsiI CCGC 4 cut(s) 243, 374, 558, 582
SspMI CTAG 2 cut(s) 392, 679
StyD4I CCNGG 2 cut(s) 349, 350
StyI CCWWGG 2 cut(s) 99, 279
TaaI ACNGT 1 cut(s) 602
TaiI ACGT 1 cut(s) 83
TaqI TCGA 3 cut(s) 11, 83, 301
TasI AATT 4 cut(s) 139, 189, 313, 462
TauI GCSGC 2 cut(s) 245, 560
Tru1I TTAA 3 cut(s) 333, 405, 659
Tru9I TTAA 3 cut(s) 333, 405, 659
TscAI CASTG 2 cut(s) 300, 393
TseFI GTSAC 2 cut(s) 26, 61
TseI GCWGC 2 cut(s) 161, 164
Tsp45I GTSAC 2 cut(s) 26, 61
TspDTI ATGAA 3 cut(s) 172, 461, 620
TspMI CCCGGG 1 cut(s) 350
TspRI CASTG 2 cut(s) 300, 393
VneI GTGCAC 1 cut(s) 76
XapI RAATTY 2 cut(s) 313, 462
XmaI CCCGGG 1 cut(s) 350
XspI CTAG 2 cut(s) 392, 679
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.