RchiOBHm_Chr7g0194341

EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0001544
Physical Location & Seq
Forward (+)
12445219 .. 12449305
4087 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ17379

Sequence Viewer

Length: 708 bp
ATGGCTGCATGTATGGCTGATGGAACAACTATACTGTCCAATGTTGCCAGAGAACCAGAGGTGGTTGATCTTGCTCGATTTCTGACTAAAAATGGGGCCTGTGTGGAAGGAGCAGGAAGCAACAAGCTTGTCATCAAGGGAAAATCTTATTTGCATGGTTGTAAATGTACTATTGCACCTGATCGTATTGAAGCAGGCACATTTATGCTTGCTGCAGCTATTACTCGCTCATTCATTTCAATTTCACCAGTCATTCCTTCCCAAGTTTCCGGTCTGATGCAAAAACTCTTGGCCGCTGGTTGCAAAATAAGGCAATGCTCTCATGATACCTTGGAAGTTTCAGCAGTGTCTCAATGTGGTGAAAATTTGCGAGGTTTTGAGGTTAAGACAGGGCCATTTCCCGGGTTTCCTACAGATCTTCAACTGCAAACAATGGCACTGCTAACCACTTGGTTTAAGTCCTGTAGAAGTCTATTTGAAAAGCGCATGGCTCATGGTATTAAACGAACTGCAGAAGCTTGGAGCAAAAATTCAAGTCTGTGCAAGCTCTGCTCTGGTTTCAGGAAAGATAATAGAAATGGTTTGTCTGGTTCCTGCCTTGCTGCAACTGACCTCAGAGGTGGGATATCATTGGTATTAGCTGCATTGGCTGCAGAAGGTACTACTGAGATCAGCGGTGTTGCTCATATTGTGGTTATGAGAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPSP_synthase

Protein Analysis

235

Amino Acids

24.98

Weight (kDa)

9.12

Isoelectric Point (pI)

37.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPSP_synthase PF00275 1 - 230 2e-33 EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 294, 675
AcoI YGGCCR 1 cut(s) 291
AcsI RAATTY 2 cut(s) 364, 529
AfaI GTAC 2 cut(s) 169, 661
AfiI CCNNNNNNNGG 1 cut(s) 401
AgsI TTSAA 5 cut(s) 191, 240, 422, 479, 534
AluBI AGCT 5 cut(s) 127, 218, 518, 547, 641
AluI AGCT 5 cut(s) 127, 218, 518, 547, 641
Alw26I GTCTC 1 cut(s) 354
Ama87I CYCGRG 1 cut(s) 401
AoxI GGCC 3 cut(s) 96, 291, 392
ApeKI GCWGC 6 cut(s) 5, 212, 215, 602, 641, 650
ApoI RAATTY 2 cut(s) 364, 529
AspLEI GCGC 1 cut(s) 486
AspS9I GGNCC 2 cut(s) 96, 392
AsuC2I CCSGG 2 cut(s) 402, 403
AsuHPI GGTGA 2 cut(s) 237, 371
AvaI CYCGRG 1 cut(s) 401
BbvI GCAGC 5 cut(s) 199, 227, 589, 628, 637
BccI CCATC 1 cut(s) 14
BcnI CCSGG 2 cut(s) 402, 403
BcoDI GTCTC 1 cut(s) 354
BfmI CTRYAG 5 cut(s) 213, 411, 463, 510, 651
BglII AGATCT 1 cut(s) 415
BisI GCNGC 7 cut(s) 6, 213, 216, 294, 603, 642, 651
BlsI GCNGC 7 cut(s) 7, 214, 217, 295, 604, 643, 652
Bme1390I CCNGG 2 cut(s) 402, 403
BmeT110I CYCGRG 1 cut(s) 401
BmgT120I GGNCC 2 cut(s) 96, 392
BmiI GGNNCC 2 cut(s) 97, 592
BmrFI CCNGG 2 cut(s) 402, 403
BmsI GCATC 1 cut(s) 267
BpuMI CCSGG 2 cut(s) 402, 403
BsaJI CCNNGG 2 cut(s) 330, 401
BsaWI WCCGGW 1 cut(s) 269
Bsc4I CCNNNNNNNGG 1 cut(s) 401
Bse1I ACTGG 1 cut(s) 248
Bse3DI GCAATG 1 cut(s) 320
BseDI CCNNGG 2 cut(s) 330, 401
BseLI CCNNNNNNNGG 1 cut(s) 401
BseMI GCAATG 1 cut(s) 320
BseMII CTCAG 2 cut(s) 628, 657
BseNI ACTGG 1 cut(s) 248
BseXI GCAGC 5 cut(s) 199, 227, 589, 628, 637
BshFI GGCC 3 cut(s) 98, 293, 394
BsiHKCI CYCGRG 1 cut(s) 401
BsiSI CCGG 2 cut(s) 270, 402
BslI CCNNNNNNNGG 1 cut(s) 401
BsmAI GTCTC 1 cut(s) 354
BsnI GGCC 3 cut(s) 98, 293, 394
BsoBI CYCGRG 1 cut(s) 401
Bsp143I GATC 4 cut(s) 67, 181, 415, 669
BspACI CCGC 2 cut(s) 294, 675
BspANI GGCC 3 cut(s) 98, 293, 394
BspCNI CTCAG 2 cut(s) 627, 658
BspHI TCATGA 1 cut(s) 322
BspLI GGNNCC 2 cut(s) 97, 592
BspMAI CTGCAG 3 cut(s) 217, 514, 655
BsrDI GCAATG 1 cut(s) 320
BsrI ACTGG 1 cut(s) 248
BssECI CCNNGG 2 cut(s) 330, 401
BssMI GATC 4 cut(s) 67, 181, 415, 669
BssT1I CCWWGG 1 cut(s) 330
Bst4CI ACNGT 1 cut(s) 36
BstAPI GCANNNNNTGC 2 cut(s) 549, 650
BstC8I GCNNGC 3 cut(s) 196, 210, 545
BstDEI CTNAG 2 cut(s) 614, 666
BstHHI GCGC 1 cut(s) 486
BstKTI GATC 4 cut(s) 70, 184, 418, 672
BstMAI GTCTC 1 cut(s) 354
BstMBI GATC 4 cut(s) 67, 181, 415, 669
BstMWI GCNNNNNNNGC 4 cut(s) 14, 549, 647, 650
BstNSI RCATGY 1 cut(s) 12
BstSCI CCNGG 2 cut(s) 400, 401
BstSFI CTRYAG 5 cut(s) 213, 411, 463, 510, 651
BstV1I GCAGC 5 cut(s) 199, 227, 589, 628, 637
BstX2I RGATCY 1 cut(s) 415
BstYI RGATCY 1 cut(s) 415
BsuRI GGCC 3 cut(s) 98, 293, 394
BtsI GCAGTG 2 cut(s) 351, 437
BtsIMutI CAGTG 2 cut(s) 351, 437
Cac8I GCNNGC 3 cut(s) 196, 210, 545
CciI TCATGA 1 cut(s) 322
CfoI GCGC 1 cut(s) 486
Cfr13I GGNCC 2 cut(s) 96, 392
Cfr9I CCCGGG 1 cut(s) 401
Csp6I GTAC 2 cut(s) 168, 660
CviAII CATG 5 cut(s) 9, 155, 323, 487, 494
CviQI GTAC 2 cut(s) 168, 660
DdeI CTNAG 2 cut(s) 614, 666
DpnI GATC 4 cut(s) 69, 183, 417, 671
DpnII GATC 4 cut(s) 67, 181, 415, 669
EaeI YGGCCR 1 cut(s) 291
Eco130I CCWWGG 1 cut(s) 330
Eco32I GATATC 1 cut(s) 627
Eco88I CYCGRG 1 cut(s) 401
EcoO109I RGGNCCY 1 cut(s) 96
EcoRV GATATC 1 cut(s) 627
EcoT14I CCWWGG 1 cut(s) 330
ErhI CCWWGG 1 cut(s) 330
FaeI CATG 5 cut(s) 12, 158, 326, 490, 497
FatI CATG 5 cut(s) 8, 154, 322, 486, 493
Fnu4HI GCNGC 7 cut(s) 6, 213, 216, 294, 603, 642, 651
Fsp4HI GCNGC 7 cut(s) 6, 213, 216, 294, 603, 642, 651
GlaI GCGC 1 cut(s) 485
GluI GCNGC 7 cut(s) 6, 213, 216, 294, 603, 642, 651
HaeIII GGCC 3 cut(s) 98, 293, 394
HapII CCGG 2 cut(s) 270, 402
HhaI GCGC 1 cut(s) 486
Hin1II CATG 5 cut(s) 12, 158, 326, 490, 497
Hin6I GCGC 1 cut(s) 484
HinP1I GCGC 1 cut(s) 484
HindIII AAGCTT 2 cut(s) 125, 516
HpaII CCGG 2 cut(s) 270, 402
HphI GGTGA 2 cut(s) 237, 371
Hpy188I TCNGA 3 cut(s) 84, 276, 617
Hpy188III TCNNGA 2 cut(s) 323, 562
HpyAV CCTTC 3 cut(s) 101, 267, 650
HpyCH4III ACNGT 1 cut(s) 36
HpyF10VI GCNNNNNNNGC 4 cut(s) 14, 549, 647, 650
HpyF3I CTNAG 2 cut(s) 614, 666
Hsp92II CATG 5 cut(s) 12, 158, 326, 490, 497
HspAI GCGC 1 cut(s) 484
Kzo9I GATC 4 cut(s) 67, 181, 415, 669
LmnI GCTCC 2 cut(s) 110, 522
Lsp1109I GCAGC 5 cut(s) 199, 227, 589, 628, 637
LweI GCATC 1 cut(s) 267
MalI GATC 4 cut(s) 69, 183, 417, 671
MboI GATC 4 cut(s) 67, 181, 415, 669
MboII GAAGA 1 cut(s) 410
MflI RGATCY 1 cut(s) 415
MluCI AATT 3 cut(s) 240, 364, 529
MnlI CCTC 5 cut(s) 52, 365, 373, 611, 623
MseI TTAA 3 cut(s) 384, 456, 501
MslI CAYNNNNRTG 1 cut(s) 203
MspA1I CMGCKG 2 cut(s) 296, 675
MspI CCGG 2 cut(s) 270, 402
MspR9I CCNGG 2 cut(s) 402, 403
MwoI GCNNNNNNNGC 4 cut(s) 14, 549, 647, 650
NciI CCSGG 2 cut(s) 402, 403
NdeII GATC 4 cut(s) 67, 181, 415, 669
NlaIII CATG 5 cut(s) 12, 158, 326, 490, 497
NlaIV GGNNCC 2 cut(s) 97, 592
NspI RCATGY 1 cut(s) 12
PagI TCATGA 1 cut(s) 322
PkrI GCNGC 7 cut(s) 7, 214, 217, 295, 604, 643, 652
PspN4I GGNNCC 2 cut(s) 97, 592
PspPI GGNCC 2 cut(s) 96, 392
PstI CTGCAG 3 cut(s) 217, 514, 655
PsuI RGATCY 1 cut(s) 415
RsaI GTAC 2 cut(s) 169, 661
RsaNI GTAC 2 cut(s) 168, 660
RseI CAYNNNNRTG 1 cut(s) 203
SaqAI TTAA 3 cut(s) 384, 456, 501
SatI GCNGC 7 cut(s) 6, 213, 216, 294, 603, 642, 651
Sau3AI GATC 4 cut(s) 67, 181, 415, 669
Sau96I GGNCC 2 cut(s) 96, 392
ScrFI CCNGG 2 cut(s) 402, 403
SfaNI GCATC 1 cut(s) 267
SfcI CTRYAG 5 cut(s) 213, 411, 463, 510, 651
SmaI CCCGGG 1 cut(s) 403
SmiMI CAYNNNNRTG 1 cut(s) 203
Sse9I AATT 3 cut(s) 240, 364, 529
SsiI CCGC 2 cut(s) 294, 675
StyD4I CCNGG 2 cut(s) 400, 401
StyI CCWWGG 1 cut(s) 330
TaaI ACNGT 1 cut(s) 36
TaqI TCGA 1 cut(s) 76
TasI AATT 3 cut(s) 240, 364, 529
TatI WGTACW 1 cut(s) 167
TauI GCSGC 1 cut(s) 296
Tru1I TTAA 3 cut(s) 384, 456, 501
Tru9I TTAA 3 cut(s) 384, 456, 501
TscAI CASTG 2 cut(s) 351, 444
TseI GCWGC 6 cut(s) 5, 212, 215, 602, 641, 650
TspDTI ATGAA 1 cut(s) 223
TspMI CCCGGG 1 cut(s) 401
TspRI CASTG 2 cut(s) 351, 444
XapI RAATTY 2 cut(s) 364, 529
XceI RCATGY 1 cut(s) 12
XmaI CCCGGG 1 cut(s) 401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.