RchiOBHm_Chr5g0031211

EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001544
Physical Location & Seq
Reverse (-)
24799863 .. 24803367
3505 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ31048

Sequence Viewer

Length: 921 bp
ATGGTTAACACTGTGGGATTACATCTGTTGAGCCTAATTCGGAAGATCCGAGGTGGGTTTTTCGTTCTTGGGCCGCTGGTAGCCCGGTTCGGTGAGGCTGTGGTTGCATTACTCGGTGGTTGTGACATTAGGGCTAGATCGGTGGACCTTTACATTCGAGGTCTTCAAGCTCTGGGTGCTGTTGTGGAGATGAAGGATGGCAAAGTTCAGGCATATGCATTAAATGGAAGAGGATTGGTTGGGGGAAGTTTCCAACTTGATTACCCTAGCATTGGGGCAACAGAAACCCTTCTGATGGCTGCATGTATGGCTGATGGAACAACTATACTGTCCAGTGTTGCCAGACAGGAAGCAACAAAGCTTGTCATCAAGGGAAAATCTTATTTTCATGGTTGTAAATGTACTATTGCACCTGATCGTATTGAAGCAGGCACATTTTTGCTTGCTGCAGCTATTGCTCGCTCATTCATTTCAATTTCACCAGTCATTCCTTCCCAAGTTTCATGTCTGATGCAAAAACTCTTGGCCGCTGGTAGCAAAATAAGGCAATGCTCTCATGATACCTTGGAAGTATTTTCAGCAGTGTCTCAATGTGGTGAGAATTTGCGAGGTTTTGAGGTTAAGACAGGGCCATTTCCCGGGTTTCCTGAAGATCTCCAACCGCAAACAATGGCCCTGCTAATCACGCGCAATGGTTTAAGTCCTGTAGAAGAGTCTGTATTTGAAAAGCGCATGGCAAATGTGGTTTGTCTGGTTCCTGCCTTGCTGCAACTGACCTCATTGGTATTAGCTGCATTGGCTGCAGAAGGTACTACTGAGATCAGCAGTGTTGCTCATATTGACCGTGGTTATGAGAATGTAGATACACTTCATATGCTGGGAGCCGATATCAAAAGATTAATGGCCCCTGCTTCTTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPSP_synthase

Protein Analysis

306

Amino Acids

32.45

Weight (kDa)

8.1

Isoelectric Point (pI)

41.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPSP_synthase PF00275 13 - 248 5.5e-29 EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 688
AciI CCGC 3 cut(s) 74, 528, 662
AclWI GGATC 1 cut(s) 40
AcoI YGGCCR 1 cut(s) 525
AcsI RAATTY 1 cut(s) 601
AcuI CTGAAG 1 cut(s) 669
AfaI GTAC 2 cut(s) 403, 811
AfiI CCNNNNNNNGG 3 cut(s) 272, 295, 638
AgsI TTSAA 4 cut(s) 167, 425, 474, 725
AluBI AGCT 4 cut(s) 170, 361, 452, 791
AluI AGCT 4 cut(s) 170, 361, 452, 791
Alw26I GTCTC 1 cut(s) 591
AlwI GGATC 1 cut(s) 40
Ama87I CYCGRG 1 cut(s) 638
AoxI GGCC 5 cut(s) 71, 525, 629, 672, 903
ApeKI GCWGC 6 cut(s) 299, 446, 449, 766, 791, 800
ApoI RAATTY 1 cut(s) 601
AseI ATTAAT 1 cut(s) 899
Asp700I GAANNNNTTC 1 cut(s) 288
AspLEI GCGC 2 cut(s) 690, 732
AspS9I GGNCC 5 cut(s) 71, 145, 629, 673, 904
AsuC2I CCSGG 3 cut(s) 85, 639, 640
AsuHPI GGTGA 3 cut(s) 104, 471, 608
AvaI CYCGRG 1 cut(s) 638
AvaII GGWCC 1 cut(s) 145
BarI GAAGNNNNNNTAC 2 cut(s) 852, 884
BbsI GAAGAC 1 cut(s) 155
BbvI GCAGC 6 cut(s) 286, 433, 461, 753, 778, 787
BccI CCATC 3 cut(s) 191, 289, 308
BcnI CCSGG 3 cut(s) 85, 639, 640
BcoDI GTCTC 1 cut(s) 591
BfaI CTAG 3 cut(s) 135, 267, 919
BfmI CTRYAG 3 cut(s) 447, 705, 801
BglII AGATCT 1 cut(s) 652
BisI GCNGC 8 cut(s) 74, 300, 447, 450, 528, 767, 792, 801
BlsI GCNGC 8 cut(s) 75, 301, 448, 451, 529, 768, 793, 802
Bme1390I CCNGG 3 cut(s) 85, 639, 640
Bme18I GGWCC 1 cut(s) 145
BmeT110I CYCGRG 1 cut(s) 638
BmgT120I GGNCC 5 cut(s) 71, 145, 629, 673, 904
BmiI GGNNCC 3 cut(s) 756, 883, 906
BmrFI CCNGG 3 cut(s) 85, 639, 640
BmsI GCATC 1 cut(s) 501
BpiI GAAGAC 1 cut(s) 155
BpuMI CCSGG 3 cut(s) 85, 639, 640
BsaJI CCNNGG 4 cut(s) 49, 564, 638, 844
Bsc4I CCNNNNNNNGG 3 cut(s) 272, 295, 638
Bse1I ACTGG 2 cut(s) 333, 482
Bse3DI GCAATG 2 cut(s) 554, 697
BseDI CCNNGG 4 cut(s) 49, 564, 638, 844
BseGI GGATG 1 cut(s) 202
BseLI CCNNNNNNNGG 3 cut(s) 272, 295, 638
BseMI GCAATG 2 cut(s) 554, 697
BseMII CTCAG 1 cut(s) 807
BseNI ACTGG 2 cut(s) 333, 482
BseXI GCAGC 6 cut(s) 286, 433, 461, 753, 778, 787
BseYI CCCAGC 1 cut(s) 877
Bsh1236I CGCG 1 cut(s) 688
BshFI GGCC 5 cut(s) 73, 527, 631, 674, 905
BsiHKCI CYCGRG 1 cut(s) 638
BsiSI CCGG 2 cut(s) 85, 639
BslI CCNNNNNNNGG 3 cut(s) 272, 295, 638
BsmAI GTCTC 1 cut(s) 591
BsnI GGCC 5 cut(s) 73, 527, 631, 674, 905
BsoBI CYCGRG 1 cut(s) 638
Bsp143I GATC 5 cut(s) 45, 137, 415, 652, 819
BspACI CCGC 3 cut(s) 74, 528, 662
BspANI GGCC 5 cut(s) 73, 527, 631, 674, 905
BspCNI CTCAG 1 cut(s) 808
BspFNI CGCG 1 cut(s) 688
BspHI TCATGA 1 cut(s) 556
BspLI GGNNCC 3 cut(s) 756, 883, 906
BspMAI CTGCAG 2 cut(s) 451, 805
BspPI GGATC 1 cut(s) 40
BsrDI GCAATG 2 cut(s) 554, 697
BsrI ACTGG 2 cut(s) 333, 482
BssECI CCNNGG 4 cut(s) 49, 564, 638, 844
BssMI GATC 5 cut(s) 45, 137, 415, 652, 819
BssT1I CCWWGG 1 cut(s) 564
Bst4CI ACNGT 3 cut(s) 13, 330, 845
Bst6I CTCTTC 2 cut(s) 223, 705
BstAPI GCANNNNNTGC 2 cut(s) 455, 800
BstC8I GCNNGC 3 cut(s) 430, 444, 460
BstDEI CTNAG 1 cut(s) 816
BstDSI CCRYGG 1 cut(s) 844
BstF5I GGATG 1 cut(s) 202
BstFNI CGCG 1 cut(s) 688
BstHHI GCGC 2 cut(s) 690, 732
BstKTI GATC 5 cut(s) 48, 140, 418, 655, 822
BstMAI GTCTC 1 cut(s) 591
BstMBI GATC 5 cut(s) 45, 137, 415, 652, 819
BstMWI GCNNNNNNNGC 7 cut(s) 104, 176, 308, 455, 685, 797, 800
BstNSI RCATGY 1 cut(s) 306
BstSCI CCNGG 3 cut(s) 83, 637, 638
BstSFI CTRYAG 3 cut(s) 447, 705, 801
BstUI CGCG 1 cut(s) 688
BstV1I GCAGC 6 cut(s) 286, 433, 461, 753, 778, 787
BstV2I GAAGAC 1 cut(s) 155
BstX2I RGATCY 2 cut(s) 45, 652
BstYI RGATCY 2 cut(s) 45, 652
BsuRI GGCC 5 cut(s) 73, 527, 631, 674, 905
BtgI CCRYGG 1 cut(s) 844
BtsCI GGATG 1 cut(s) 202
BtsI GCAGTG 2 cut(s) 588, 832
BtsIMutI CAGTG 4 cut(s) 9, 340, 588, 832
Cac8I GCNNGC 3 cut(s) 430, 444, 460
CciI TCATGA 1 cut(s) 556
CfoI GCGC 2 cut(s) 690, 732
Cfr13I GGNCC 5 cut(s) 71, 145, 629, 673, 904
Cfr9I CCCGGG 1 cut(s) 638
Csp6I GTAC 2 cut(s) 402, 810
CviAII CATG 5 cut(s) 303, 389, 504, 557, 733
CviQI GTAC 2 cut(s) 402, 810
DdeI CTNAG 1 cut(s) 816
DpnI GATC 5 cut(s) 47, 139, 417, 654, 821
DpnII GATC 5 cut(s) 45, 137, 415, 652, 819
EaeI YGGCCR 1 cut(s) 525
Eam1104I CTCTTC 2 cut(s) 223, 705
EarI CTCTTC 2 cut(s) 223, 705
Eco130I CCWWGG 1 cut(s) 564
Eco32I GATATC 1 cut(s) 889
Eco47I GGWCC 1 cut(s) 145
Eco57I CTGAAG 1 cut(s) 669
Eco88I CYCGRG 1 cut(s) 638
EcoRV GATATC 1 cut(s) 889
EcoT14I CCWWGG 1 cut(s) 564
EcoT22I ATGCAT 1 cut(s) 220
ErhI CCWWGG 1 cut(s) 564
FaeI CATG 5 cut(s) 306, 392, 507, 560, 736
FatI CATG 5 cut(s) 302, 388, 503, 556, 732
FauNDI CATATG 2 cut(s) 214, 873
Fnu4HI GCNGC 8 cut(s) 74, 300, 447, 450, 528, 767, 792, 801
FokI GGATG 1 cut(s) 209
Fsp4HI GCNGC 8 cut(s) 74, 300, 447, 450, 528, 767, 792, 801
FspBI CTAG 3 cut(s) 135, 267, 919
GlaI GCGC 2 cut(s) 689, 731
GluI GCNGC 8 cut(s) 74, 300, 447, 450, 528, 767, 792, 801
GsaI CCCAGC 1 cut(s) 881
HaeIII GGCC 5 cut(s) 73, 527, 631, 674, 905
HapII CCGG 2 cut(s) 85, 639
HhaI GCGC 2 cut(s) 690, 732
Hin1II CATG 5 cut(s) 306, 392, 507, 560, 736
Hin6I GCGC 2 cut(s) 688, 730
HinP1I GCGC 2 cut(s) 688, 730
HincII GTYRAC 1 cut(s) 7
HindII GTYRAC 1 cut(s) 7
HindIII AAGCTT 1 cut(s) 359
HinfI GANTC 1 cut(s) 713
HpaI GTTAAC 1 cut(s) 7
HpaII CCGG 2 cut(s) 85, 639
HphI GGTGA 3 cut(s) 104, 471, 608
Hpy166II GTNNAC 2 cut(s) 7, 145
Hpy188I TCNGA 4 cut(s) 42, 50, 294, 510
Hpy188III TCNNGA 2 cut(s) 557, 647
Hpy8I GTNNAC 2 cut(s) 7, 145
HpyAV CCTTC 4 cut(s) 187, 299, 501, 800
HpyCH4III ACNGT 3 cut(s) 13, 330, 845
HpyCH4V TGCA 9 cut(s) 107, 218, 302, 410, 449, 514, 769, 794, 803
HpyF10VI GCNNNNNNNGC 7 cut(s) 104, 176, 308, 455, 685, 797, 800
HpyF3I CTNAG 1 cut(s) 816
Hsp92II CATG 5 cut(s) 306, 392, 507, 560, 736
HspAI GCGC 2 cut(s) 688, 730
KspAI GTTAAC 1 cut(s) 7
Kzo9I GATC 5 cut(s) 45, 137, 415, 652, 819
LmnI GCTCC 1 cut(s) 881
Lsp1109I GCAGC 6 cut(s) 286, 433, 461, 753, 778, 787
LweI GCATC 1 cut(s) 501
MaeI CTAG 3 cut(s) 135, 267, 919
MaeIII GTNAC 1 cut(s) 122
MalI GATC 5 cut(s) 47, 139, 417, 654, 821
MboI GATC 5 cut(s) 45, 137, 415, 652, 819
MboII GAAGA 6 cut(s) 55, 155, 240, 662, 722, 906
MflI RGATCY 2 cut(s) 45, 652
MluCI AATT 3 cut(s) 36, 474, 601
MlyI GAGTC 1 cut(s) 722
MmeI TCCRAC 2 cut(s) 277, 682
MnlI CCTC 7 cut(s) 44, 88, 152, 224, 602, 610, 787
Mph1103I ATGCAT 1 cut(s) 220
MroXI GAANNNNTTC 1 cut(s) 288
MseI TTAA 5 cut(s) 6, 221, 621, 698, 899
MspA1I CMGCKG 2 cut(s) 76, 530
MspI CCGG 2 cut(s) 85, 639
MspR9I CCNGG 3 cut(s) 85, 639, 640
MvnI CGCG 1 cut(s) 688
MwoI GCNNNNNNNGC 7 cut(s) 104, 176, 308, 455, 685, 797, 800
NciI CCSGG 3 cut(s) 85, 639, 640
NdeI CATATG 2 cut(s) 214, 873
NdeII GATC 5 cut(s) 45, 137, 415, 652, 819
NlaIII CATG 5 cut(s) 306, 392, 507, 560, 736
NlaIV GGNNCC 3 cut(s) 756, 883, 906
NmuCI GTSAC 1 cut(s) 122
NsiI ATGCAT 1 cut(s) 220
NspI RCATGY 1 cut(s) 306
PagI TCATGA 1 cut(s) 556
PcsI WCGNNNNNNNCGW 1 cut(s) 46
PdmI GAANNNNTTC 1 cut(s) 288
PkrI GCNGC 8 cut(s) 75, 301, 448, 451, 529, 768, 793, 802
PleI GAGTC 1 cut(s) 721
PpsI GAGTC 1 cut(s) 721
PshBI ATTAAT 1 cut(s) 899
PspFI CCCAGC 1 cut(s) 877
PspN4I GGNNCC 3 cut(s) 756, 883, 906
PspPI GGNCC 5 cut(s) 71, 145, 629, 673, 904
PstI CTGCAG 2 cut(s) 451, 805
PsuI RGATCY 2 cut(s) 45, 652
RsaI GTAC 2 cut(s) 403, 811
RsaNI GTAC 2 cut(s) 402, 810
SaqAI TTAA 5 cut(s) 6, 221, 621, 698, 899
SatI GCNGC 8 cut(s) 74, 300, 447, 450, 528, 767, 792, 801
Sau3AI GATC 5 cut(s) 45, 137, 415, 652, 819
Sau96I GGNCC 5 cut(s) 71, 145, 629, 673, 904
SchI GAGTC 1 cut(s) 722
ScrFI CCNGG 3 cut(s) 85, 639, 640
SfaNI GCATC 1 cut(s) 501
SfcI CTRYAG 3 cut(s) 447, 705, 801
SinI GGWCC 1 cut(s) 145
SmaI CCCGGG 1 cut(s) 640
Sse9I AATT 3 cut(s) 36, 474, 601
SsiI CCGC 3 cut(s) 74, 528, 662
SspMI CTAG 3 cut(s) 135, 267, 919
StyD4I CCNGG 3 cut(s) 83, 637, 638
StyI CCWWGG 1 cut(s) 564
TaaI ACNGT 3 cut(s) 13, 330, 845
TaqI TCGA 1 cut(s) 157
TasI AATT 3 cut(s) 36, 474, 601
TatI WGTACW 1 cut(s) 401
TauI GCSGC 2 cut(s) 76, 530
Tru1I TTAA 5 cut(s) 6, 221, 621, 698, 899
Tru9I TTAA 5 cut(s) 6, 221, 621, 698, 899
TscAI CASTG 4 cut(s) 16, 340, 588, 832
TseFI GTSAC 1 cut(s) 122
TseI GCWGC 6 cut(s) 299, 446, 449, 766, 791, 800
Tsp45I GTSAC 1 cut(s) 122
TspDTI ATGAA 5 cut(s) 206, 377, 457, 492, 860
TspMI CCCGGG 1 cut(s) 638
TspRI CASTG 4 cut(s) 16, 340, 588, 832
VpaK11BI GGWCC 1 cut(s) 145
VspI ATTAAT 1 cut(s) 899
XapI RAATTY 1 cut(s) 601
XceI RCATGY 1 cut(s) 306
XmaI CCCGGG 1 cut(s) 638
XmnI GAANNNNTTC 1 cut(s) 288
XspI CTAG 3 cut(s) 135, 267, 919
Zsp2I ATGCAT 1 cut(s) 220
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.